Ana-Maria Ene - Academia.edu (original) (raw)

Papers by Ana-Maria Ene

Research paper thumbnail of Histopathological diagnosis of cutaneous vascular sarcomas

Romanian Journal of Morphology and Embryology Revue Roumaine De Morphologie Et Embryologie, 2010

Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigate... more Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigates the histopathological and immunohistochemical features in different cases of angiosarcoma and Kaposi's sarcoma (cutaneous vascular sarcomas), which are representative for medical practice. The clinical-histopathological-immunohistochemical correlations render possible the differential diagnosis and a proper treatment can be applied to obtain a favorable prognosis.

Research paper thumbnail of Apoptosis in cutaneous melanomas

Romanian Journal of Morphology and Embryology Revue Roumaine De Morphologie Et Embryologie, Feb 1, 2007

Cutaneous melanomas has become one of the most discussed and studied tumor because its particular... more Cutaneous melanomas has become one of the most discussed and studied tumor because its particular immunologic development but also its increasing rate worldwide in the last decades. Even thought many patients are diagnosed at an early stage, the death rate continues to rise due to the increasing incidence of more advanced lesions. The aim of this study is to detect apoptosis in 30 cases of cutaneous melanomas using the in situ end-labeling technique (TUNEL) who quantify apoptotic cell death at single cell level and tissues.

Research paper thumbnail of Thin coatings based on ZnO@C18-usnic acid nanoparticles prepared by MAPLE inhibit the development of Salmonela enterica early biofilm growth

Applied Surface Science, 2015

Research paper thumbnail of Neuroprotective and Free Radicals Scavenging Potentials of Some Common Leafy Vegetables Consumed in South - Eastern Nigeria

Annual Research & Review in Biology, 2014

Antioxidant potential, lipid peroxidation inhibition ability in brain homogenate and total phenol... more Antioxidant potential, lipid peroxidation inhibition ability in brain homogenate and total phenolic and flavonoid contents of ethanol extracts of some leafy vegetables (Vernonia amygdalina, Gongronema latifolium and Ocimum gratissimum) consumed in south-eastern Nigeria were determined. The antioxidant and neuroprotective potentials of the ethanol extracts were assessed by 2, 2-diphenyl-1-picrylhydrazil (DPPH) assay and through determination of thiobarbituric acid (TBA) reactive substances in brain homogenates. Total phenolic and flavonoid contents were quantitatively determined in the extracts spectrophotometrically. Results obtained from the present study showed that G. latifolium and V. amygdalina extracts were effective against hydrogen peroxide-induced lipid peroxidation with threshold inhibitory concentrations (IC 50 ) values of 357.36±15.6µg/ml and 629.5±22.72µg/ml respectively while O. gratissimum achieved only 20% inhibition (IC 20 ) at 785.73±40.23µg/ml. In the DPPH free radical scavenging assay, 50% of DPPH radicals were scavenged at181.5(µg/ml), 153.33(µg/ml), and 189.67(µg/ml) of V. amygdalina, G. latifolium and O. gratissimum extracts concentrations respectively. Free radical scavenging ability of the extracts was in the order G. Latifolium >V. Amygdalina > O. gratissimum. Total phenolic content was 432.79±42.2mg/100g,274.1±37.3mg/100g, and 490.49±58.1mg/100g catechin equivalent, while flavonoid content was 843.09±21.6 mg/100g, 746.34±13.7mg/100g and 1160.98±24.5mg/100g catechin equivalent in V. amygdalina, G. latifoliumand O. gratissimum respectively. The inhibition of lipid peroxidation and DPPH radical scavenging followed a logistic dose response pattern.These results indicatethat these vegetables possess neuro-protective potentials and free radical scavenging ability. The present data scientifically corroborate with the consumption of these plants as an exogenous sources of antioxidants that could improve nutrition and boost antioxidant defense.

Research paper thumbnail of Histopathological diagnosis of cutaneous vascular sarcomas

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2010

Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigate... more Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigates the histopathological and immunohistochemical features in different cases of angiosarcoma and Kaposi's sarcoma (cutaneous vascular sarcomas), which are representative for medical practice. The clinical-histopathological-immunohistochemical correlations render possible the differential diagnosis and a proper treatment can be applied to obtain a favorable prognosis.

Research paper thumbnail of Apoptosis in cutaneous melanomas

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2007

Cutaneous melanomas has become one of the most discussed and studied tumor because its particular... more Cutaneous melanomas has become one of the most discussed and studied tumor because its particular immunologic development but also its increasing rate worldwide in the last decades. Even thought many patients are diagnosed at an early stage, the death rate continues to rise due to the increasing incidence of more advanced lesions. The aim of this study is to detect apoptosis in 30 cases of cutaneous melanomas using the in situ end-labeling technique (TUNEL) who quantify apoptotic cell death at single cell level and tissues.

Research paper thumbnail of Role of Notch signaling into cutaneous melanoma development and progression

Research paper thumbnail of The His1069Gln mutation in the ATP7B gene in Romanian patients with Wilson's disease referred to a tertiary gastroenterology center

Journal of gastrointestinal and liver diseases : JGLD, 2012

Wilson's disease (WD) is a rare autosomal recessive disease. More than 500 mutations have bee... more Wilson's disease (WD) is a rare autosomal recessive disease. More than 500 mutations have been described so far, out of which 29 in exon 14. H1069Q mutation in the exon 14 of ATP7B gene is the most frequently encountered in Europe. The aim of the present study was to evaluate the incidence of mutations occurring in exon 14 of ATP7B gene in Romanian patients referred to a tertiary gastroenterology center, with known or suspected WD and in asymptomatic first degree relatives of index cases. 93 patients were included in the study. Exon 14 of ATP7B gene has been amplified by PCR from genomic DNA and mutations identified by sequencing. Only H1069Q missense mutation was detected in our study group. In patients with an established diagnosis of WD (38 cases), 34.2% were heterozygous for H1069Q and 21.1% were homozygous, with an allelic frequency of 38.1%. In paediatric WD patients (12 cases) 25% were heterozygous and 16.7% were homozygous (not significant versus adult population). Among...

Research paper thumbnail of MicroRNA Expression Profiles in Kaposi’s Sarcoma

Pathology & Oncology Research, 2013

Kaposi's sarcoma (KS) is a mesenchymal tumor, caused by Human herpesvirus 8 (HHV8) with molecular... more Kaposi's sarcoma (KS) is a mesenchymal tumor, caused by Human herpesvirus 8 (HHV8) with molecular and cytogenetic changes poorly understood. To gain further insight on the underlying molecular changes in KS, we performed microRNA (miRNA) microarray analysis of 17 Kaposi's sarcoma specimens. Three normal skin specimens were used as controls. The most significant differentially expressed miRNA were confirmed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR). We detected in KS versus normal skin 185 differentially expressed miRNAs, 76 were upregulated and 109 were downregulated. The most significantly downregulated miRNAs were miR-99a, miR-200 family, miR-199b-5p, miR-100 and miR-335, whereas kshv-miR-K12-4-3p, kshv-miR-K12-1, kshv-miR-K12-2, kshv-miR-K12-4-5p and kshv-miR-K12-8 were significantly upregulated. High expression levels of kshv-miR-K12-1 (p=0.004) and kshv-miR-K12-4-3p (p=0.001) was confirmed by RT-PCR. The predicted target genes for differentially expressed miRNAs included genes which are involved in a variety of cellular processes such as angiogenesis (i.e. THBS1) and apoptosis (i.e. CASP3, MCL1), suggesting a role for these miRNAs in Kaposi's sarcoma pathogenesis.

Research paper thumbnail of Histopathological diagnosis of cutaneous vascular sarcomas

Romanian Journal of Morphology and Embryology Revue Roumaine De Morphologie Et Embryologie, 2010

Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigate... more Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigates the histopathological and immunohistochemical features in different cases of angiosarcoma and Kaposi's sarcoma (cutaneous vascular sarcomas), which are representative for medical practice. The clinical-histopathological-immunohistochemical correlations render possible the differential diagnosis and a proper treatment can be applied to obtain a favorable prognosis.

Research paper thumbnail of Apoptosis in cutaneous melanomas

Romanian Journal of Morphology and Embryology Revue Roumaine De Morphologie Et Embryologie, Feb 1, 2007

Cutaneous melanomas has become one of the most discussed and studied tumor because its particular... more Cutaneous melanomas has become one of the most discussed and studied tumor because its particular immunologic development but also its increasing rate worldwide in the last decades. Even thought many patients are diagnosed at an early stage, the death rate continues to rise due to the increasing incidence of more advanced lesions. The aim of this study is to detect apoptosis in 30 cases of cutaneous melanomas using the in situ end-labeling technique (TUNEL) who quantify apoptotic cell death at single cell level and tissues.

Research paper thumbnail of Thin coatings based on ZnO@C18-usnic acid nanoparticles prepared by MAPLE inhibit the development of Salmonela enterica early biofilm growth

Applied Surface Science, 2015

Research paper thumbnail of Neuroprotective and Free Radicals Scavenging Potentials of Some Common Leafy Vegetables Consumed in South - Eastern Nigeria

Annual Research & Review in Biology, 2014

Antioxidant potential, lipid peroxidation inhibition ability in brain homogenate and total phenol... more Antioxidant potential, lipid peroxidation inhibition ability in brain homogenate and total phenolic and flavonoid contents of ethanol extracts of some leafy vegetables (Vernonia amygdalina, Gongronema latifolium and Ocimum gratissimum) consumed in south-eastern Nigeria were determined. The antioxidant and neuroprotective potentials of the ethanol extracts were assessed by 2, 2-diphenyl-1-picrylhydrazil (DPPH) assay and through determination of thiobarbituric acid (TBA) reactive substances in brain homogenates. Total phenolic and flavonoid contents were quantitatively determined in the extracts spectrophotometrically. Results obtained from the present study showed that G. latifolium and V. amygdalina extracts were effective against hydrogen peroxide-induced lipid peroxidation with threshold inhibitory concentrations (IC 50 ) values of 357.36±15.6µg/ml and 629.5±22.72µg/ml respectively while O. gratissimum achieved only 20% inhibition (IC 20 ) at 785.73±40.23µg/ml. In the DPPH free radical scavenging assay, 50% of DPPH radicals were scavenged at181.5(µg/ml), 153.33(µg/ml), and 189.67(µg/ml) of V. amygdalina, G. latifolium and O. gratissimum extracts concentrations respectively. Free radical scavenging ability of the extracts was in the order G. Latifolium >V. Amygdalina > O. gratissimum. Total phenolic content was 432.79±42.2mg/100g,274.1±37.3mg/100g, and 490.49±58.1mg/100g catechin equivalent, while flavonoid content was 843.09±21.6 mg/100g, 746.34±13.7mg/100g and 1160.98±24.5mg/100g catechin equivalent in V. amygdalina, G. latifoliumand O. gratissimum respectively. The inhibition of lipid peroxidation and DPPH radical scavenging followed a logistic dose response pattern.These results indicatethat these vegetables possess neuro-protective potentials and free radical scavenging ability. The present data scientifically corroborate with the consumption of these plants as an exogenous sources of antioxidants that could improve nutrition and boost antioxidant defense.

Research paper thumbnail of Histopathological diagnosis of cutaneous vascular sarcomas

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2010

Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigate... more Cutaneous sarcomas represent a heterogeneous group of mesenchymal lesions. This study investigates the histopathological and immunohistochemical features in different cases of angiosarcoma and Kaposi's sarcoma (cutaneous vascular sarcomas), which are representative for medical practice. The clinical-histopathological-immunohistochemical correlations render possible the differential diagnosis and a proper treatment can be applied to obtain a favorable prognosis.

Research paper thumbnail of Apoptosis in cutaneous melanomas

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2007

Cutaneous melanomas has become one of the most discussed and studied tumor because its particular... more Cutaneous melanomas has become one of the most discussed and studied tumor because its particular immunologic development but also its increasing rate worldwide in the last decades. Even thought many patients are diagnosed at an early stage, the death rate continues to rise due to the increasing incidence of more advanced lesions. The aim of this study is to detect apoptosis in 30 cases of cutaneous melanomas using the in situ end-labeling technique (TUNEL) who quantify apoptotic cell death at single cell level and tissues.

Research paper thumbnail of Role of Notch signaling into cutaneous melanoma development and progression

Research paper thumbnail of The His1069Gln mutation in the ATP7B gene in Romanian patients with Wilson's disease referred to a tertiary gastroenterology center

Journal of gastrointestinal and liver diseases : JGLD, 2012

Wilson's disease (WD) is a rare autosomal recessive disease. More than 500 mutations have bee... more Wilson's disease (WD) is a rare autosomal recessive disease. More than 500 mutations have been described so far, out of which 29 in exon 14. H1069Q mutation in the exon 14 of ATP7B gene is the most frequently encountered in Europe. The aim of the present study was to evaluate the incidence of mutations occurring in exon 14 of ATP7B gene in Romanian patients referred to a tertiary gastroenterology center, with known or suspected WD and in asymptomatic first degree relatives of index cases. 93 patients were included in the study. Exon 14 of ATP7B gene has been amplified by PCR from genomic DNA and mutations identified by sequencing. Only H1069Q missense mutation was detected in our study group. In patients with an established diagnosis of WD (38 cases), 34.2% were heterozygous for H1069Q and 21.1% were homozygous, with an allelic frequency of 38.1%. In paediatric WD patients (12 cases) 25% were heterozygous and 16.7% were homozygous (not significant versus adult population). Among...

Research paper thumbnail of MicroRNA Expression Profiles in Kaposi’s Sarcoma

Pathology & Oncology Research, 2013

Kaposi's sarcoma (KS) is a mesenchymal tumor, caused by Human herpesvirus 8 (HHV8) with molecular... more Kaposi's sarcoma (KS) is a mesenchymal tumor, caused by Human herpesvirus 8 (HHV8) with molecular and cytogenetic changes poorly understood. To gain further insight on the underlying molecular changes in KS, we performed microRNA (miRNA) microarray analysis of 17 Kaposi's sarcoma specimens. Three normal skin specimens were used as controls. The most significant differentially expressed miRNA were confirmed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR). We detected in KS versus normal skin 185 differentially expressed miRNAs, 76 were upregulated and 109 were downregulated. The most significantly downregulated miRNAs were miR-99a, miR-200 family, miR-199b-5p, miR-100 and miR-335, whereas kshv-miR-K12-4-3p, kshv-miR-K12-1, kshv-miR-K12-2, kshv-miR-K12-4-5p and kshv-miR-K12-8 were significantly upregulated. High expression levels of kshv-miR-K12-1 (p=0.004) and kshv-miR-K12-4-3p (p=0.001) was confirmed by RT-PCR. The predicted target genes for differentially expressed miRNAs included genes which are involved in a variety of cellular processes such as angiogenesis (i.e. THBS1) and apoptosis (i.e. CASP3, MCL1), suggesting a role for these miRNAs in Kaposi's sarcoma pathogenesis.