Ana Uetanabaro - Academia.edu (original) (raw)

Papers by Ana Uetanabaro

Research paper thumbnail of Structure of the sting apparatus and associated exocrine glands in Dinoponera quadriceps (Santschi, 1921) (Hymenoptera: Formicidae)

Boletin De La Sea, 2014

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Research paper thumbnail of BIOSSEGURANcCA NA PRODUcCÃO DA CACHAcCA DE ALAMBIQUE

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Research paper thumbnail of Leveduras killer: biologia, ecologia e aplicações biotecnológicas

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Research paper thumbnail of Comparative study in vivo between the zinc phosphate cement and resin cement

The cementation of the fi xed partial prosthesis to the prepared tooth is an important step, beca... more The cementation of the fi xed partial prosthesis to the prepared tooth is an important step, because through this way the small area between the artifi cial element and the teeth structure is closed, protecting it from the chemical, physical and bacterial nature of irritable elements. The study aims to anylise the superfi cial bacterial colonization Qualitatively of both zinc

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Research paper thumbnail of Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides

Pharmaceutical Biology, 2015

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Research paper thumbnail of Chemical composition and antibacterial activity of the essential oil from Mentha requienii Bentham

Natural product research, 2013

The chemical composition of essential oil obtained by hydrodistillation of the fresh aerial parts... more The chemical composition of essential oil obtained by hydrodistillation of the fresh aerial parts of Mentha requienii Bentham (Lamiaceae) collected on the Gennargentu Mountains (Sardinia, Italy) has been investigated by gas chromatography and gas chromatography-mass spectrometry. The main constituents that resulted were pulegone (78%), menthone (0.5%), isomenthone (18%), isopulegone (1.3%) and limonene (1.76%). In vitro antifungal activity is evaluated in order to identify new means that could be helpful in the prevention of contamination in indoor environments.

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Research paper thumbnail of Pharmacological Basis for Traditional Use of the Lippia thymoides

Evidence-Based Complementary and Alternative Medicine, 2015

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Research paper thumbnail of Influence of bacteria from the duodenal microbiota of patients with symptomatic giardiasis on the pathogenicity of Giardia duodenalis in gnotoxenic mice

Journal of medical microbiology, 2000

Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but n... more Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were isolated from the dominant duodenal microbiota of five patients with symptomatic giardiasis. The bacterial combinations from each patient were associated with groups (GN) of germ-free mice. Five days after the association, when their faecal populations ranged from 10(7) to 10(9) cfu/g, all groups were inoculated intragastrically with 10(5) viable trophozoites of G. duodenalis strain BT6. Two groups of germ-free (GF) and conventional (CV1) mice were also infected. Gnotobiotic animals were killed 10 days after infection and GF and CV1 animals were killed 10, 20 and 30 days after infection. More marked pathological alterations were detected in CV1 mice when compared with GF ...

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Research paper thumbnail of Chemical composition and antibacterial activity of essential oils from Myrcia alagoensis (Myrtaceae)

Natural product communications, 2013

The chemical composition and antibacterial activity of essential oils obtained from fresh and dri... more The chemical composition and antibacterial activity of essential oils obtained from fresh and dried leaves of Myrcia alagoensis O. Berg, collected in a secondary forest remnant in north-eastern Brazil, was compared. The essential oils were obtained by hydrodistillation from fresh and dried leaves, and analysed by GC/FID and GC/MS. The antimicrobial properties of the oils were investigated against five bacteria by determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The essential oils were rich in cyclic sesquiterpenes, such as germacrene B, with antibiotic action against Gram-positive and Gram-negative bacteria. The drying process after collection interfered with the chemical composition and antibacterial activity of the assessed samples.

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Research paper thumbnail of In vitro Antifungal Activity of Irlbachia purpurascens, Lantana macrophylla and Kielmeyera neglecta Extracts Against Candida Isolates Collected from Patients with Vulvovaginal Candidiasis

Research Journal of Medicinal Plant, 2013

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Research paper thumbnail of Isolation and identification of endophytic fungi in the medicinal plant Mikania laevigata (Asteraceae)

Pharmacognosy Journal, 2014

Holcoglossum is one of the smaller genera of Orchidaceae, mainly distributed in southwest China. ... more Holcoglossum is one of the smaller genera of Orchidaceae, mainly distributed in southwest China. Some members of this genus as well as H. rupestre and H. flavescens are endemic and rare Chinese orchids. As far as we know, little work has been done concerning the relationships between the Holcoglossum plants and endophytic microorganisms. In this study, 46 culturable fungal endophytes were isolated and identified from roots of nine Holcoglossum plants collected from Yunnan, Guangxi, and Hainan provinces of China based on molecular techniques. The results showed that all strains belonged to four classes, i.e., Sordariomycetes (41.30%), Dothideomycetes (36.96%), Agaricomycetes (17.39%), Leotiomycetes (4.35%). Thirty-six strains were identified at the genus level, including Alternaria, Cladosporium, Clonostachys, Colletotrichum, Cosmospora, Cryptosporiopsis, Cylindrocarpon, Didymella, Epulorhiza (Anamorphic Tulasnella), Fusarium, Myrmecridium, Leptosphaeria, Paraconiothyrium, Phomopsis, Pyrenochaeta, and Stephanonectria. Fusarium and Epulorhiza (Anamorphic Tulasnella) were the dominant fungal endophytes. Some orchids mycorrhizal fungi as well as Tulasnella calospora and Epulorhiza sp. were found in roots. This is the first report concerning endophytic fungi from Holcoglossum plants (Orchidaceae), suggesting that endophytic fungi in Holcoglossum plants are very abundant.

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Research paper thumbnail of Essential oils and isolated compounds from Lippia alba leaves and flowers: Antimicrobial activity and osteoclast apoptosis

International Journal of Molecular Medicine, 2014

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Research paper thumbnail of Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches' broom disease of cacao

Journal of microbiology (Seoul, Korea), 2009

Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin,... more Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transc...

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Research paper thumbnail of The Benefits of Probiotics in Human and Animal Nutrition

New Advances in the Basic and Clinical Gastroenterology, 2012

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Research paper thumbnail of Atividade antimicrobiana de méis de cinco espécies de abelhas brasileiras sem ferrão

Ciência Rural, 2013

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Research paper thumbnail of Xylariaceae Endophytic Fungi Metabolites Against Salmonella

Salmonella - A Diversified Superbug, 2012

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Research paper thumbnail of 91. Proteomic analyses of the Venom from the Giant Ant Dinoponera quadriceps: A Comparative Study and Characterization of the Major Components of the Venom Derived from 4 Different Areas of Brazil

Toxicon, 2012

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Research paper thumbnail of Volatile and non-volatile compounds and antimicrobial activity of Mansoa difficilis (Cham.) Bureau & K. Schum: (Bignoniaceae)

Química Nova, 2012

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Research paper thumbnail of Antimicrobial activity of Marcetia DC species (Melastomataceae) and analysis of its flavonoids by reverse phase-high performance liquid chromatography coupled-diode array detector

Pharmacognosy Magazine, 2012

Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil),... more Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil), and most are endemic to the highlands of the Chapada Diamantina (Bahia). Among the species, only M. taxifolia (A.St.-Hil.) DC. populates Brazil (state of Roraima to Paraná) and also Venezuela, Colombia, and Guyana. This work evaluated the antimicrobial activity of hexane, ethyl acetate, and methanol extracts of three species of Marcetia (Marcetia canescens Naud., M. macrophylla Wurdack, and M. taxifolia A.StHil) against several microorganism. In addition, the flavonoids were analyzed in extracts by HPLC-DAD. The tests were made using Gram-positive (three strains of Staphylococcus aureus) and Gram-negative (two strains of Escherichia coli, a strain of Pseudomonas aeruginosa and another of Salmonella choleraesius) bacteria resistant and nonresistant to antibiotics and yeasts (two strains of Candida albicans and one of C. parapsilosis) by the disk diffusion method. Solid-phase extraction (SPE) was performed on the above extracts to isolate flavonoids, which were subsequently analyzed by high performance liquid chromatography coupled diode array detector (HPLC-DAD). Results showed that extracts inhibited the Gram-positive bacteria and yeast. The hexane extracts possessed the lowest activity, while the ethyl acetate and methanolic extracts were more active. Marcetia taxifolia was more effective (active against 10 microorganisms studied), and only its methanol extract inhibited Gram-negative bacteria (P. aeruginosa and S. choleraesius). SPE and HPLC-DAD analysis showed that M. canescens and M. macrophylla contain glycosylated flavonoids, while the majority of extracts from M. taxifolia were aglycone flavonoids.

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Research paper thumbnail of Volatiles, A Glutarimide Alkaloid and Antimicrobial Effects of Croton pullei (Euphorbiaceae)

Molecules, 2013

Chemical investigation of Croton pullei (Euphorbiaceae) collected in the Brazilian Amazon region ... more Chemical investigation of Croton pullei (Euphorbiaceae) collected in the Brazilian Amazon region was revisited. The chemical composition of the essential oils of leaves and stems was analyzed by GC/MS. It was found that both the oils comprise mainly terpenes, among which linalool was the major one (24.90 and 39.72%, respectively). Phytochemical investigation of the stem methanol extract led to the isolation of a new natural product from the glutarimide alkaloid group named N-[2,6-dioxo-1-(2-phenylethyl)-3-piperidinyl]-acetamide, confirming that C. pullei is a rich source of this class of alkaloids. The hexane and methanol extracts of the stems of C. pullei showed moderate antibacterial and antifungal activity and the highest inhibition was observed when the methanol extract was tested against Staphylococcus aureus CCMB 262 and CCMB 263.

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Research paper thumbnail of Structure of the sting apparatus and associated exocrine glands in Dinoponera quadriceps (Santschi, 1921) (Hymenoptera: Formicidae)

Boletin De La Sea, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of BIOSSEGURANcCA NA PRODUcCÃO DA CACHAcCA DE ALAMBIQUE

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Leveduras killer: biologia, ecologia e aplicações biotecnológicas

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Comparative study in vivo between the zinc phosphate cement and resin cement

The cementation of the fi xed partial prosthesis to the prepared tooth is an important step, beca... more The cementation of the fi xed partial prosthesis to the prepared tooth is an important step, because through this way the small area between the artifi cial element and the teeth structure is closed, protecting it from the chemical, physical and bacterial nature of irritable elements. The study aims to anylise the superfi cial bacterial colonization Qualitatively of both zinc

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides

Pharmaceutical Biology, 2015

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Chemical composition and antibacterial activity of the essential oil from Mentha requienii Bentham

Natural product research, 2013

The chemical composition of essential oil obtained by hydrodistillation of the fresh aerial parts... more The chemical composition of essential oil obtained by hydrodistillation of the fresh aerial parts of Mentha requienii Bentham (Lamiaceae) collected on the Gennargentu Mountains (Sardinia, Italy) has been investigated by gas chromatography and gas chromatography-mass spectrometry. The main constituents that resulted were pulegone (78%), menthone (0.5%), isomenthone (18%), isopulegone (1.3%) and limonene (1.76%). In vitro antifungal activity is evaluated in order to identify new means that could be helpful in the prevention of contamination in indoor environments.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Pharmacological Basis for Traditional Use of the Lippia thymoides

Evidence-Based Complementary and Alternative Medicine, 2015

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Research paper thumbnail of Influence of bacteria from the duodenal microbiota of patients with symptomatic giardiasis on the pathogenicity of Giardia duodenalis in gnotoxenic mice

Journal of medical microbiology, 2000

Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but n... more Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were isolated from the dominant duodenal microbiota of five patients with symptomatic giardiasis. The bacterial combinations from each patient were associated with groups (GN) of germ-free mice. Five days after the association, when their faecal populations ranged from 10(7) to 10(9) cfu/g, all groups were inoculated intragastrically with 10(5) viable trophozoites of G. duodenalis strain BT6. Two groups of germ-free (GF) and conventional (CV1) mice were also infected. Gnotobiotic animals were killed 10 days after infection and GF and CV1 animals were killed 10, 20 and 30 days after infection. More marked pathological alterations were detected in CV1 mice when compared with GF ...

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Research paper thumbnail of Chemical composition and antibacterial activity of essential oils from Myrcia alagoensis (Myrtaceae)

Natural product communications, 2013

The chemical composition and antibacterial activity of essential oils obtained from fresh and dri... more The chemical composition and antibacterial activity of essential oils obtained from fresh and dried leaves of Myrcia alagoensis O. Berg, collected in a secondary forest remnant in north-eastern Brazil, was compared. The essential oils were obtained by hydrodistillation from fresh and dried leaves, and analysed by GC/FID and GC/MS. The antimicrobial properties of the oils were investigated against five bacteria by determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The essential oils were rich in cyclic sesquiterpenes, such as germacrene B, with antibiotic action against Gram-positive and Gram-negative bacteria. The drying process after collection interfered with the chemical composition and antibacterial activity of the assessed samples.

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Research paper thumbnail of In vitro Antifungal Activity of Irlbachia purpurascens, Lantana macrophylla and Kielmeyera neglecta Extracts Against Candida Isolates Collected from Patients with Vulvovaginal Candidiasis

Research Journal of Medicinal Plant, 2013

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Research paper thumbnail of Isolation and identification of endophytic fungi in the medicinal plant Mikania laevigata (Asteraceae)

Pharmacognosy Journal, 2014

Holcoglossum is one of the smaller genera of Orchidaceae, mainly distributed in southwest China. ... more Holcoglossum is one of the smaller genera of Orchidaceae, mainly distributed in southwest China. Some members of this genus as well as H. rupestre and H. flavescens are endemic and rare Chinese orchids. As far as we know, little work has been done concerning the relationships between the Holcoglossum plants and endophytic microorganisms. In this study, 46 culturable fungal endophytes were isolated and identified from roots of nine Holcoglossum plants collected from Yunnan, Guangxi, and Hainan provinces of China based on molecular techniques. The results showed that all strains belonged to four classes, i.e., Sordariomycetes (41.30%), Dothideomycetes (36.96%), Agaricomycetes (17.39%), Leotiomycetes (4.35%). Thirty-six strains were identified at the genus level, including Alternaria, Cladosporium, Clonostachys, Colletotrichum, Cosmospora, Cryptosporiopsis, Cylindrocarpon, Didymella, Epulorhiza (Anamorphic Tulasnella), Fusarium, Myrmecridium, Leptosphaeria, Paraconiothyrium, Phomopsis, Pyrenochaeta, and Stephanonectria. Fusarium and Epulorhiza (Anamorphic Tulasnella) were the dominant fungal endophytes. Some orchids mycorrhizal fungi as well as Tulasnella calospora and Epulorhiza sp. were found in roots. This is the first report concerning endophytic fungi from Holcoglossum plants (Orchidaceae), suggesting that endophytic fungi in Holcoglossum plants are very abundant.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Essential oils and isolated compounds from Lippia alba leaves and flowers: Antimicrobial activity and osteoclast apoptosis

International Journal of Molecular Medicine, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches' broom disease of cacao

Journal of microbiology (Seoul, Korea), 2009

Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin,... more Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transc...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The Benefits of Probiotics in Human and Animal Nutrition

New Advances in the Basic and Clinical Gastroenterology, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Atividade antimicrobiana de méis de cinco espécies de abelhas brasileiras sem ferrão

Ciência Rural, 2013

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Xylariaceae Endophytic Fungi Metabolites Against Salmonella

Salmonella - A Diversified Superbug, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 91. Proteomic analyses of the Venom from the Giant Ant Dinoponera quadriceps: A Comparative Study and Characterization of the Major Components of the Venom Derived from 4 Different Areas of Brazil

Toxicon, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Volatile and non-volatile compounds and antimicrobial activity of Mansoa difficilis (Cham.) Bureau & K. Schum: (Bignoniaceae)

Química Nova, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Antimicrobial activity of Marcetia DC species (Melastomataceae) and analysis of its flavonoids by reverse phase-high performance liquid chromatography coupled-diode array detector

Pharmacognosy Magazine, 2012

Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil),... more Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil), and most are endemic to the highlands of the Chapada Diamantina (Bahia). Among the species, only M. taxifolia (A.St.-Hil.) DC. populates Brazil (state of Roraima to Paraná) and also Venezuela, Colombia, and Guyana. This work evaluated the antimicrobial activity of hexane, ethyl acetate, and methanol extracts of three species of Marcetia (Marcetia canescens Naud., M. macrophylla Wurdack, and M. taxifolia A.StHil) against several microorganism. In addition, the flavonoids were analyzed in extracts by HPLC-DAD. The tests were made using Gram-positive (three strains of Staphylococcus aureus) and Gram-negative (two strains of Escherichia coli, a strain of Pseudomonas aeruginosa and another of Salmonella choleraesius) bacteria resistant and nonresistant to antibiotics and yeasts (two strains of Candida albicans and one of C. parapsilosis) by the disk diffusion method. Solid-phase extraction (SPE) was performed on the above extracts to isolate flavonoids, which were subsequently analyzed by high performance liquid chromatography coupled diode array detector (HPLC-DAD). Results showed that extracts inhibited the Gram-positive bacteria and yeast. The hexane extracts possessed the lowest activity, while the ethyl acetate and methanolic extracts were more active. Marcetia taxifolia was more effective (active against 10 microorganisms studied), and only its methanol extract inhibited Gram-negative bacteria (P. aeruginosa and S. choleraesius). SPE and HPLC-DAD analysis showed that M. canescens and M. macrophylla contain glycosylated flavonoids, while the majority of extracts from M. taxifolia were aglycone flavonoids.

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Research paper thumbnail of Volatiles, A Glutarimide Alkaloid and Antimicrobial Effects of Croton pullei (Euphorbiaceae)

Molecules, 2013

Chemical investigation of Croton pullei (Euphorbiaceae) collected in the Brazilian Amazon region ... more Chemical investigation of Croton pullei (Euphorbiaceae) collected in the Brazilian Amazon region was revisited. The chemical composition of the essential oils of leaves and stems was analyzed by GC/MS. It was found that both the oils comprise mainly terpenes, among which linalool was the major one (24.90 and 39.72%, respectively). Phytochemical investigation of the stem methanol extract led to the isolation of a new natural product from the glutarimide alkaloid group named N-[2,6-dioxo-1-(2-phenylethyl)-3-piperidinyl]-acetamide, confirming that C. pullei is a rich source of this class of alkaloids. The hexane and methanol extracts of the stems of C. pullei showed moderate antibacterial and antifungal activity and the highest inhibition was observed when the methanol extract was tested against Staphylococcus aureus CCMB 262 and CCMB 263.

Bookmarks Related papers MentionsView impact