Ana Uetanabaro - Profile on Academia.edu (original) (raw)

Papers by Ana Uetanabaro

Structure of the sting apparatus and associated exocrine glands in Dinoponera quadriceps (Santschi, 1921) (Hymenoptera: Formicidae)

Boletin De La Sea, 2014

BIOSSEGURANcCA NA PRODUcCÃO DA CACHAcCA DE ALAMBIQUE

Leveduras killer: biologia, ecologia e aplicações biotecnológicas) -A atividade killer de levedur... more Leveduras killer: biologia, ecologia e aplicações biotecnológicas) -A atividade killer de leveduras tem sido observada em mais de 90 espécies e diversas toxinas killer (micocinas) produzidas por linhagens pertencentes a diversos gêneros têm sido extensivamente estudadas. Muitos estudos têm destacado as aplicações biotecnológicas do fenômeno killer: (i) controle de leveduras contaminantes de processos fermentativos; (ii) taxonomia e biotipagem de leveduras de interesse clínico e industrial; e (iii) aplicações biomédicas como novos agentes antimicóticos. Este trabalho de revisão aborda as potenciais aplicações do sistema killer de leveduras com base em estudos até o momento realizados, demonstrando o potencial biotecnológico dessas leveduras.

Comparative study in vivo between the zinc phosphate cement and resin cement

The cementation of the fi xed partial prosthesis to the prepared tooth is an important step, beca... more The cementation of the fi xed partial prosthesis to the prepared tooth is an important step, because through this way the small area between the artifi cial element and the teeth structure is closed, protecting it from the chemical, physical and bacterial nature of irritable elements. The study aims to anylise the superfi cial bacterial colonization Qualitatively of both zinc

Pharmaceutical Biology, 2015

Context: Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds,... more Context: Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds, fever, bronchitis, rheumatism, headaches, and weakness. Objective: This study determinates the chemical composition of essential oils from L. thymoides, obtained at during each of the four seasons and correlates with pharmacological properties. Materials and methods: Essential oils were obtained by hydrodistillation and analyzed by gas chromatography coupled to mass spectroscopy (GC-MS). Antioxidant activity was determined by DPPH free radical scavenging and b-carotene bleaching methods. The antimicrobial assays were performed by minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) methods. Isolated rat aorta and uterus, and guinea-pig trachea were utilized to evaluate relaxant potential in pre-contracted smooth muscle. Results and discussion: Essential oils from leaves of L. thymoides had the sesquiterpene b-caryophyllene (17.22-26.27%) as the major constituent followed by borneol (4.45-7.36%), camphor (3.22-8.61%), camphene (2.64-5.66%), and germacrene D (4.72-6.18%). In vitro assays showed that these essential oils do not have antioxidant activity, have antimicrobial selectivity to Gram-positive bacteria Staphylococcus aureus (MIC ¼ 0.004 mg/mL and MMC ¼ 0.26-10.19 mg/mL) and Micrococcus luteus (MIC ¼ 0.03 mg/mL and MMC ¼ 8.43 mg/mL), relax isolated rat aorta (EC 50 ¼ 305-544 mg/mL, with endothelium; and EC 50 ¼ 150-283 mg/mL, without endothelium), and uterus (EC 50 ¼ 74-257 mg/mL), and minor potency, isolated guinea-pig trachea. Conclusions: Lippia thymoides is a source of natural products of pharmaceutical interest, being necessary additional studies to determine the substances involved in the biological activities.

Chemical composition and antibacterial activity of the essential oil from Mentha requienii Bentham

Natural product research, 2013

The chemical composition of essential oil obtained by hydrodistillation of the fresh aerial parts... more The chemical composition of essential oil obtained by hydrodistillation of the fresh aerial parts of Mentha requienii Bentham (Lamiaceae) collected on the Gennargentu Mountains (Sardinia, Italy) has been investigated by gas chromatography and gas chromatography-mass spectrometry. The main constituents that resulted were pulegone (78%), menthone (0.5%), isomenthone (18%), isopulegone (1.3%) and limonene (1.76%). In vitro antifungal activity is evaluated in order to identify new means that could be helpful in the prevention of contamination in indoor environments.

Evidence-Based Complementary and Alternative Medicine, 2015

Journal of medical microbiology, 2000

Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but n... more Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were isolated from the dominant duodenal microbiota of five patients with symptomatic giardiasis. The bacterial combinations from each patient were associated with groups (GN) of germ-free mice. Five days after the association, when their faecal populations ranged from 10(7) to 10(9) cfu/g, all groups were inoculated intragastrically with 10(5) viable trophozoites of G. duodenalis strain BT6. Two groups of germ-free (GF) and conventional (CV1) mice were also infected. Gnotobiotic animals were killed 10 days after infection and GF and CV1 animals were killed 10, 20 and 30 days after infection. More marked pathological alterations were detected in CV1 mice when compared with GF ...

Chemical composition and antibacterial activity of essential oils from Myrcia alagoensis (Myrtaceae)

Natural product communications, 2013

The chemical composition and antibacterial activity of essential oils obtained from fresh and dri... more The chemical composition and antibacterial activity of essential oils obtained from fresh and dried leaves of Myrcia alagoensis O. Berg, collected in a secondary forest remnant in north-eastern Brazil, was compared. The essential oils were obtained by hydrodistillation from fresh and dried leaves, and analysed by GC/FID and GC/MS. The antimicrobial properties of the oils were investigated against five bacteria by determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The essential oils were rich in cyclic sesquiterpenes, such as germacrene B, with antibiotic action against Gram-positive and Gram-negative bacteria. The drying process after collection interfered with the chemical composition and antibacterial activity of the assessed samples.

Research Journal of Medicinal Plant, 2013

Isolation and identification of endophytic fungi in the medicinal plant Mikania laevigata (Asteraceae)

Pharmacognosy Journal, 2014

Holcoglossum is one of the smaller genera of Orchidaceae, mainly distributed in southwest China. ... more Holcoglossum is one of the smaller genera of Orchidaceae, mainly distributed in southwest China. Some members of this genus as well as H. rupestre and H. flavescens are endemic and rare Chinese orchids. As far as we know, little work has been done concerning the relationships between the Holcoglossum plants and endophytic microorganisms. In this study, 46 culturable fungal endophytes were isolated and identified from roots of nine Holcoglossum plants collected from Yunnan, Guangxi, and Hainan provinces of China based on molecular techniques. The results showed that all strains belonged to four classes, i.e., Sordariomycetes (41.30%), Dothideomycetes (36.96%), Agaricomycetes (17.39%), Leotiomycetes (4.35%). Thirty-six strains were identified at the genus level, including Alternaria, Cladosporium, Clonostachys, Colletotrichum, Cosmospora, Cryptosporiopsis, Cylindrocarpon, Didymella, Epulorhiza (Anamorphic Tulasnella), Fusarium, Myrmecridium, Leptosphaeria, Paraconiothyrium, Phomopsis, Pyrenochaeta, and Stephanonectria. Fusarium and Epulorhiza (Anamorphic Tulasnella) were the dominant fungal endophytes. Some orchids mycorrhizal fungi as well as Tulasnella calospora and Epulorhiza sp. were found in roots. This is the first report concerning endophytic fungi from Holcoglossum plants (Orchidaceae), suggesting that endophytic fungi in Holcoglossum plants are very abundant.

International Journal of Molecular Medicine, 2014

In the present study, essential oils extracted from the leaves and lowers of Lippia alba (Mill.) ... more In the present study, essential oils extracted from the leaves and lowers of Lippia alba (Mill.) N.E.Br. (L. alba) were analyzed for their antimicrobial activity and their effects on osteoclasts. The periodontal pathogens, Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans; ATCC 43717), Fusobacterium nucleatum (F. nucleatum; ATCC 25586) and Porphyromonas gingivalis (P. gingivalis); ATCC 33277) were used in antimicrobial activity assays for determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), whereas Bacteroides fragilis (B. fragilis; ATCC 25285) was used as the control microorganism. Osteoclast (OC) apoptosis was assessed by TUNEL assay and Fas receptor expression was detected by immunocytochemistry. The analysis of antimicrobial activity revealed that P. gingivalis had the lowest MIC values, whereas A. actinomycetemcomitans had the highest. L. alba essential oils were found to be toxic to human cells, although the compounds, carvone, limonene and citral, were non-toxic and induced apoptosis in the OCs. This study demonstrates that L. alba has potential biotechnological application in dentistry. In fact periodontal disease has a multifactorial etiology, and the immune response to microbial challenge leads to osteoclast activation and the resorption of the alveolar bone, resulting in tooth loss.

Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches' broom disease of cacao

Journal of microbiology (Seoul, Korea), 2009

Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin,... more Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transc...

New Advances in the Basic and Clinical Gastroenterology, 2012

Ciência Rural, 2013

A atividade antimicrobiana de méis produzidos por Melipona asilvai, Melipona quadrifasciata anthi... more A atividade antimicrobiana de méis produzidos por Melipona asilvai, Melipona quadrifasciata anthidioides, Friseomelita doederleinei, Tetragonisca angustula e Plebeia sp. foi investigada. O teste de difusão em poço demonstrou que todos os méis tem ação antibacteriana frente a Staphylococcus aureus, mas somente as amostras produzidas por M. quadrifasciata anthidioides e F. doederleinei inibiram o crescimento de Escherichia coli. No ensaio de determinação da concentração inibitória mínima, os méis de M. asilvai, M. quadrifasciata anthidioides, F. doederleinei e T. angustula foram mais ativos que os de Plebeia sp. frente a S. aureus e E. coli. Os microorganismos Pseudomonas aeruginosa e Candida albicans foram resistentes a todos os méis em ambos ensaios. Os méis foram mais efetivos contra as bactérias do que frente a uma solução de açúcar, sugerindo que o mecanismo de inibição do crescimento bacteriano não está somente relacionado ao efeito osmótico. Os resultados obtidos podem explicar o uso medicinal desses méis em doenças bacterianas. Palavras-chave: abelha sem ferrão, atividade antibacteriana, efeito osmótico, mel. ABSTRACT The antimicrobial activity of honey produced by Melipona asilvai, Melipona quadrifasciata anthidioides, Friseomelita doederleinei, Tetragonisca angustula and Plebeia sp. were investigated. The agar well diffusion assay demonstrated that all honeys had antibacterial activity against Staphylococcus aureus, but only the samples from M. quadrifasciata anthidioides and F. doederleinei inhibited the growth of Escherichia coli. In the Minimum Inhibitory Concentration determination assay, M. asilvai, M. quadrifasciata anthidioides, F. doederleinei and T. angustula honeys were more active than that from Plebeia sp. for S. aureus and E. coli. The microorganisms Pseudomonas aeruginosa and Candida albicans were resistant to the all native stingless bee honeys in both assays. Honeys were more effective against bacteria than a sugar solution, suggesting that the mechanism for bacterial growth inhibition is not only related to the osmotic effect. The results of antimicrobial activity may explain the popular medicinal use of these honeys in bacterial diseases.

Salmonella - A Diversified Superbug, 2012

91. Proteomic analyses of the Venom from the Giant Ant Dinoponera quadriceps: A Comparative Study and Characterization of the Major Components of the Venom Derived from 4 Different Areas of Brazil

Toxicon, 2012

Química Nova, 2012

Recebido em 30/4/12; aceito em 1/10/12; publicado na web em 9/11/12 Essential oil from the leaves... more Recebido em 30/4/12; aceito em 1/10/12; publicado na web em 9/11/12 Essential oil from the leaves of Mansoa difficilis was analyzed by GC/MS. Oct-1-en-3-ol (49.65%) was the major compound, but diallyl di-and trisulfide were also present (0.85 and 0.37%, respectively), justifying the garlic-like odor of the crushed leaves. The hexane and methanol extracts of the leaves and stems afforded as main constituents a mixture of linear hydrocarbons, spinasterol, stigmasterol, ursolic and oleanolic acids, two apigenin derivatives and verbascoside. The hexane and methanol extracts of leaves were tested for antimicrobial activity against ten microorganisms. The hexane extract was active against both Psedomonas aeruginosa and Staphylococcus aureus.

Antimicrobial activity of Marcetia DC species (Melastomataceae) and analysis of its flavonoids by reverse phase-high performance liquid chromatography coupled-diode array detector

Pharmacognosy Magazine, 2012

Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil),... more Marcetia genera currently comprises 29 species, with approximately 90% inhabiting Bahia (Brazil), and most are endemic to the highlands of the Chapada Diamantina (Bahia). Among the species, only M. taxifolia (A.St.-Hil.) DC. populates Brazil (state of Roraima to Paraná) and also Venezuela, Colombia, and Guyana. This work evaluated the antimicrobial activity of hexane, ethyl acetate, and methanol extracts of three species of Marcetia (Marcetia canescens Naud., M. macrophylla Wurdack, and M. taxifolia A.StHil) against several microorganism. In addition, the flavonoids were analyzed in extracts by HPLC-DAD. The tests were made using Gram-positive (three strains of Staphylococcus aureus) and Gram-negative (two strains of Escherichia coli, a strain of Pseudomonas aeruginosa and another of Salmonella choleraesius) bacteria resistant and nonresistant to antibiotics and yeasts (two strains of Candida albicans and one of C. parapsilosis) by the disk diffusion method. Solid-phase extraction (SPE) was performed on the above extracts to isolate flavonoids, which were subsequently analyzed by high performance liquid chromatography coupled diode array detector (HPLC-DAD). Results showed that extracts inhibited the Gram-positive bacteria and yeast. The hexane extracts possessed the lowest activity, while the ethyl acetate and methanolic extracts were more active. Marcetia taxifolia was more effective (active against 10 microorganisms studied), and only its methanol extract inhibited Gram-negative bacteria (P. aeruginosa and S. choleraesius). SPE and HPLC-DAD analysis showed that M. canescens and M. macrophylla contain glycosylated flavonoids, while the majority of extracts from M. taxifolia were aglycone flavonoids.

Volatiles, A Glutarimide Alkaloid and Antimicrobial Effects of Croton pullei (Euphorbiaceae)

Molecules, 2013

Chemical investigation of Croton pullei (Euphorbiaceae) collected in the Brazilian Amazon region ... more Chemical investigation of Croton pullei (Euphorbiaceae) collected in the Brazilian Amazon region was revisited. The chemical composition of the essential oils of leaves and stems was analyzed by GC/MS. It was found that both the oils comprise mainly terpenes, among which linalool was the major one (24.90 and 39.72%, respectively). Phytochemical investigation of the stem methanol extract led to the isolation of a new natural product from the glutarimide alkaloid group named N-[2,6-dioxo-1-(2-phenylethyl)-3-piperidinyl]-acetamide, confirming that C. pullei is a rich source of this class of alkaloids. The hexane and methanol extracts of the stems of C. pullei showed moderate antibacterial and antifungal activity and the highest inhibition was observed when the methanol extract was tested against Staphylococcus aureus CCMB 262 and CCMB 263.