Ana Velasco-Ramírez - Academia.edu (original) (raw)

Papers by Ana Velasco-Ramírez

Research paper thumbnail of Evaluación del efecto rizogénico de productos orgánicos en dos tipos de sustrato en esquejes de hortensias (Hydrangea macrophylla)

E-Cucba, Jun 29, 2023

vegetales ornamentales, la hortensia (Hydrangea macrophylla) es una de las más llamativas y popul... more vegetales ornamentales, la hortensia (Hydrangea macrophylla) es una de las más llamativas y populares, debido a su belleza y el tamaño de sus inflorescencias. Su producción comienza regularmente a partir de esquejes terminales que deben enraizarse. En el presente trabajo, se probó la capacidad rizogénica de cuatro productos comerciales a base de reguladores de crecimiento y dos productos orgánicos: parénquima medular de sábila y extracto de algas aplicados en dos sustratos. Los resultados encontrados, mostraron la eficiencia de la sábila para producir callo en los esquejes y mantener la turgencia de los mismos, lo cual implica que ésta pueda ser usada a pequeña y mediana escala en el enraizamiento de esquejes herbáceos como los de la Hydrangea macrophylla.

Research paper thumbnail of Uso en medicina tradicional de Verbesina sphaerocephala A. Gray (Asteraceae) en la comunidad de San Martin de las Flores, Jalisco, México

Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants, Mar 30, 2019

The community of San Martin de las Flores, Jalisco, México has a varied flora, part of which is u... more The community of San Martin de las Flores, Jalisco, México has a varied flora, part of which is used by its residents to treat their diseases. One of these wild species that grows in this community is Verbesina sphaerocephala A.Gray (Asteraceae). The objective of this research was to provide an overview of local knowledge about the traditional uses of this plant. A quantitative ethnobotanical method to analyse the value of traditional use of this species among the community, as well as, bibliographic information on the existing phytochemical and pharmacological properties of species of the genus Verbesina has been used. When examining the general knowledge of V. sphaerocephala among the community, it was concluded that the plant is used mainly in traditional medicine to treat eight different types of diseases, highlighting its use as a wound healing and anti-inflammatory.

Research paper thumbnail of Genetic variability of sargassum liebmannii on the coast of jalisco in the central mexican pacific revealed by molecular markers and morphological traits

Electronic Journal of Biotechnology, Aug 1, 2021

Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwellin... more Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwelling areas in the tropical Mexican Pacific. This brown algae is of great environmental and industrial importance. However, no information is available that documents the genetic or phenotypic variability of the species, which is needed to determine how it may react to environmental variation related to climate change. In this study, S. liebmannii specimens were collected from the coast of Jalisco, Mexico, and a molecular and morphological characterization was conducted. Intraspecific variability was estimated according to the study areas. Results The inter-simple sequence repeat (ISSR) markers indicated a polymorphism percentage of 95%. The Shannon index and Nei index showed relatively low values among the populations (0.3569 and 0.081, respectively). On the other hand, the genetic differentiation coefficient indicated inter- and intrapopulation values of 36.69% and 63.31%, respectively. The Jaccard similarity coefficient was used to determine the degree of similarity among individuals by geographical area. The morphological characteristics and environmental variables that were used to correlate phenotypes and genotypes indicated that S. liebmannii showed low genetic flow due to the presence of geographical barriers due to substrate that was not optimal for algal development. Conclusions The ISSR markers were useful for detecting genetic differences among S. liebmannii individuals. The results indicate that a coupled genotypic-phenotypic study is beneficial for documenting the variation present in little-studied algal species. These studies may be used in future research to clarify taxonomic controversies while generating additional genomic information.

Research paper thumbnail of Application of Seaweed Generates Changes in the Substrate and Stimulates the Growth of Tomato Plants

Plants, Mar 31, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of EFFECT OF LIQUID SEAWEED EXTRACT ON POTTED GROWTH OF Eustoma grandiflorum (Raf.) Shinners)

Tropical and Subtropical Agroecosystems, Jun 10, 2020

Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, im... more Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, improves crop development and consequently yield, since by stimulating natural processes they benefit from the use of nutrients and increases resistance to conditions of biotic and / or abiotic stress. Objective. This investigation evaluated the response of two varieties of Eustoma grandiflorum to foliar and root application of algae extracts, grown in pots under nursery conditions. Methodology. Macroalgae extract was applied in a foliar way (20 mL spray) and directly to the substrate (50 mL), while drinking water was applied as the control. Results. The parameters obtained indicate that the foliar application has noticeably effects on var. Rosie for the variables root length, number of shoots, and number of leaves; while for var. Florida, the variables total plant length and number of shoots obtained greater significance. The method of application via substrate generates positive effects on the total length and stem length for var. Rosie, although the variables with the greatest noticeably were obtained from var. Florida for root length, number of leaves, and flower diameter. Implications. The low doses of the algae extracts, application form and the handling of cultural tasks during the growth of E. grandiflorum, contribute to a better absorption and/or efficiency of the fertilizers in the crop. Conclusion. The application of algae extracts favors the production of E. grandiflorum under nursery conditions, making their use a viable option to minimize the application of conventional fertilizers, thereby attenuating the effects on the environment.

Research paper thumbnail of Evaluación del efecto rizogénico de productos orgánicos en dos tipos de sustrato en esquejes de hortensias (Hydrangea macrophylla)

E-CUCBA

Within the industry for the production of ornamental plant species, the hydrangea (Hydrangea macr... more Within the industry for the production of ornamental plant species, the hydrangea (Hydrangea macrophylla) is one of the moststriking and popular, due to its beauty and the size of its inflorescences. Its production starts regularly from terminal cuttings thatneed to be rooted. In the present work, the rhizogenic capacity of four commercial products based on growth regulators and twoorganic products was tested: aloe medullary parenchyma and algae extract. The results found showed the efficiency of aloe vera toproduce callus in the cuttings and maintain their turgidity, which implies that it can be used on a small and medium scale in therooting of herbaceous cuttings such as those of Hydrangea macrophylla.

Research paper thumbnail of EFFECT OF LIQUID SEAWEED EXTRACT ON POTTED GROWTH OF Eustoma grandiflorum (Raf.) Shinners)

Tropical and Subtropical Agroecosystems

Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, im... more Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, improves crop development and consequently yield, since by stimulating natural processes they benefit from the use of nutrients and increases resistance to conditions of biotic and / or abiotic stress. Objective. This investigation evaluated the response of two varieties of Eustoma grandiflorum to foliar and root application of algae extracts, grown in pots under nursery conditions. Methodology. Macroalgae extract was applied in a foliar way (20 mL spray) and directly to the substrate (50 mL), while drinking water was applied as the control. Results. The parameters obtained indicate that the foliar application has noticeably effects on var. Rosie for the variables root length, number of shoots, and number of leaves; while for var. Florida, the variables total plant length and number of shoots obtained greater significance. The method of application via substrate generates positive effects ...

Research paper thumbnail of Application of Seaweed Generates Changes in the Substrate and Stimulates the Growth of Tomato Plants

Plants

Ulva ohnoi is a cosmopolitan green seaweed with commercial potential given the biomass that may b... more Ulva ohnoi is a cosmopolitan green seaweed with commercial potential given the biomass that may be generated. We evaluated the effects of substrate changes induced by U. ohnoi application on the vegetative response of tomato plants under greenhouse conditions. First, the decomposition dynamics and N release of the dry seaweed biomass were studied using the litterbag method. Subsequently, we evaluated the effect of seaweed powder (SP) or seaweed extract (SE) applications on substrate and plant growth. Additionally, the growth parameters responses evaluated were related to the changes in substrate properties associated with each treatment. The results showed that the dry seaweed biomass has a rapid rate of degradation (k = 0.07 day−1) and N release (k = 0.024 day−1). The SP application improved the physicochemical and biological characteristics of the substrate by increasing the availability of minerals, the fungi:bacteria ratio, and the growth morphophysiological parameters (length, ...

Research paper thumbnail of Verbesina sphaerocephala andVerbesina fastigiata extracts promote the pot development of Fragaria ananassa

Agroindustrial science, Apr 5, 2022

Esta obra está publicada bajo la licencia CC BY-NC-4.0 Extractos de Verbesina sphaerocephala y Ve... more Esta obra está publicada bajo la licencia CC BY-NC-4.0 Extractos de Verbesina sphaerocephala y Verbesina fastigiata promueven el desarrollo en maceta de Fragaria ananassa Verbesina sphaerocephala and Verbesina fastigiata extracts promote the pot development of Fragaria ananassa

Research paper thumbnail of Presence of arsenic and potentially toxic metals (Cd, Cr, Pb) in water and soil of the NE shore of Chapala Lake, Mexico, and its genotoxic effect in the edible chayote fruit (Sechium edule (Jacq.) Sw.)

European Journal of Horticultural Science, 2020

The presence of potentially toxic elements in waters used for agricultural irrigation is a latent... more The presence of potentially toxic elements in waters used for agricultural irrigation is a latent problem in Lake Chapala, Mexico. Four sites in the polluted area of Chapala lake were studied in this research. Samples of irrigation water and soil were collected from each site in both the dry (DS) and rainy (RS) seasons. Arsenic (As), cadmium (Cd), chromium (Cr), and lead (Pb) were measured in all the samples. Comet assay was used to measure the migration of DNA from the nucleus in order to detect genotoxicity in fruits. ISSR molecular markers were used to assess the detection power of this marker in correspondence with amounts of metals in the samples. The results show a variation of metal concentrations in the DS and RS soil and water samples. The metalloid As in DS water samples exceeded the permissible limits in three of the sampling areas. Cd and Pb exceeded the prescribed limits from collected water in three of the sampling areas in RS. Cd exceeded the maximum permissible limits in soil collected during both DS and RS in the four sampling sites. Data on the concentration of metals shows significant differences between the sampling areas and the control site. Genetic damage (DNA migration from the nuclei) was found in the chayote fruit and the major migration of DNA was associated principally with the presence of As in DS water samples as well as Pb in soil in RS.

Research paper thumbnail of Effects of Nanomaterials/Nanocomposites on Trace Element Uptake and Phytotoxicity

Nanomaterials and Nanocomposites Exposures to Plants

Research paper thumbnail of Estudio preliminar de identificación a nivel molecular, de ecotipos de chile piquín

e-CUCBA

The piquín chili is a plant genetic resource with great potential for culinary nutrition and vita... more The piquín chili is a plant genetic resource with great potential for culinary nutrition and vitamin supply for consumer countriessuch as Mexico. Its study at the molecular level represents a possibility of differentiating the existing ecotypes in each region whereit is produced or where it is consumed. Due to the great morphological variability that it presents, the molecular characterization ofchili as a plant genetic resource represents obtaining the characteristics from the genetic point of view. This is necessary for theidentification of promising genotypes for genetic improvement in addition to documentation of the gene pool. In the present work, apreliminary study was carried out to identify piquín chili ecotypes from nine states of the republic, using the molecular markerISSR (Inter Simple Sequence Repeats). The results obtained provide information on the ability of molecular markers to detectdifferences in ecotypes, which correspond to the different collection regions.

Research paper thumbnail of EFFECT OF DAMINOZIDE ON THE GROWTH AND FLOWERING OF Eustoma grandiflorum PROPAGATED IN POTTED

Acta Scientiarum Polonorum Hortorum Cultus

Daminozide (B-Nine) it is a plant growth regulator, is translocated from the leaves to the stem t... more Daminozide (B-Nine) it is a plant growth regulator, is translocated from the leaves to the stem to avoid elongation and produce more compact plants and uniform growth and vigor. For the cultivation of Lisiathus the information of the damonizide application is scarce despite of great marketing potential, so the objective of this study was to evaluate the effect of daminozide on the vegetative growth and flowering of Lisianthus grown in pot. Granulated Lisianthus seeds were germinated and transplanted into pots under nursery conditions. Three foliar applications of daminozide were realized; two applications in vegetative stage and one in flowering, adding three concentrations in each of them (0.5, 1.0 and 1.5 ml L–1) were used and water was applied as a control during the vegetative and flowering phases. The applications of daminozide at 1.0 and 1.5 ml L–1 were optimal for the vegetative development in Lisianthus (plant height – 15 cm, size of the internodes – 2.5 cm, number of branch...

Research paper thumbnail of The Impact of Aqueous Extracts of Verbesina sphaerocephala and Verbesina fastigiata on Germination and Growth in Solanum lycopersicum and Cucumis sativus Seedlings

Horticulturae

The use of extracts derived from different plants has gained popularity as an alternative option ... more The use of extracts derived from different plants has gained popularity as an alternative option to manage weeds and support phenological development in plants. One of the main problems facing agricultural production is the intensive application of chemical fertilizers that harm the environment. This study investigated the biostimulant effects of the aqueous extracts of Verbesinasphaerocephala and Verbesinafastigiata on the germination and growth of tomato (Solanum lycopersicum) and American cucumber (Cucumis sativus) seedlings. The aqueous extracts of both species of Verbesina were tested at concentrations of 0.5 and 1%. Seed germination and seedling development under nursery conditions were evaluated. The seed germination percentage was reduced in all treatments with the extracts of both Verbesina species when compared to that of the control treatment, which was considered an allelopathic effect. However, seedling growth in pots showed different behavior, with the extracts benefic...

Research paper thumbnail of Uso en Medicina Tradicional De Verbesina Sphaerocephala a Gray Asteraceae en La Comunidad De San Martin De Las Flores Jalisco Mexico

Universidad de Santiago de Chile, Mar 30, 2019

Research paper thumbnail of Genetic variability of Sargassum liebmannii on the coast of Jalisco in the central Mexican Pacific revealed by molecular markers and morphological traits

Electronic Journal of Biotechnology, 2021

Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwellin... more Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwelling areas in the tropical Mexican Pacific. This brown algae is of great environmental and industrial importance. However, no information is available that documents the genetic or phenotypic variability of the species, which is needed to determine how it may react to environmental variation related to climate change. In this study, S. liebmannii specimens were collected from the coast of Jalisco, Mexico, and a molecular and morphological characterization was conducted. Intraspecific variability was estimated according to the study areas. Results The inter-simple sequence repeat (ISSR) markers indicated a polymorphism percentage of 95%. The Shannon index and Nei index showed relatively low values among the populations (0.3569 and 0.081, respectively). On the other hand, the genetic differentiation coefficient indicated inter- and intrapopulation values of 36.69% and 63.31%, respectively. The Jaccard similarity coefficient was used to determine the degree of similarity among individuals by geographical area. The morphological characteristics and environmental variables that were used to correlate phenotypes and genotypes indicated that S. liebmannii showed low genetic flow due to the presence of geographical barriers due to substrate that was not optimal for algal development. Conclusions The ISSR markers were useful for detecting genetic differences among S. liebmannii individuals. The results indicate that a coupled genotypic-phenotypic study is beneficial for documenting the variation present in little-studied algal species. These studies may be used in future research to clarify taxonomic controversies while generating additional genomic information.

Research paper thumbnail of Verbesina sphaerocephala extracts promote the development of Cucumis sativus and reduce the damage caused by Meloidogyne incognita

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology, 2021

Uno de los principales problemas fitosanitarios son los nematodos agalladores del género Meloidog... more Uno de los principales problemas fitosanitarios son los nematodos agalladores del género Meloidogyne. El uso de Verbesina sphaerocephala podrían sustituir el uso de nematicidas químicos. El objetivo de esta investigación fue probar si extractos acuosos de V. sphaerocephala promueven el desarrollo y reducen los daños ocasionados por M. incognita en Cucumis sativus. Se evaluaron siete tratamientos en plantas de C. sativus crecidas en macetas en condiciones de invernadero: plantas testigo sin nematodos, plantas con nematodos, con nematodos + carbofuran, con nematodos + Trichoderma, con nematodos + extracto de V. sphaerocephala (concentración al 10, 15 y 20%). Se realizó tres evaluaciones para estimar altura de planta, diámetro de tallo, concentración de clorofila, número de hojas y flores, longitud y peso de raíces, índice de agallamiento, huevos en raíz y larvas en el suelo. Los resultaron mostraron que los extractos de V. sphaerocephala al 10 y 15% promovieron el crecimiento de las p...

Research paper thumbnail of Genetic structure of Zephyranthes fosteri, specie with ornamental and medicinal potential in Mexico

Biotecnia, 2019

Zephyranthes fosteri is a wild species distributed in Mexico, it is popular for its ornamental qu... more Zephyranthes fosteri is a wild species distributed in Mexico, it is popular for its ornamental quality. Anthropogenic activities perturbed its natural habitats and threaten its survival. At present time, there is not enough information about the genetic relationships and structure among Z. fosteri distributed in Mexico. The objective of this investigation was to elucidate the genetic relationships and structure among accessions of Z. fosteri collected in western and southeastern Mexico. ISSR markers were used to establish the genetic variability and genetic structure among 60 accessions randomly collected in western Mexico and one population found in southeastern Mexico. Jaccard’s coefficient and AMOVA analysis determined the similarity and variability among and within accessions, and Bayesian model was used to assess the genetic structure. A mean heterozygosity of 0.49 was found indicated a moderate variability. The AMOVA analysis showed that 75 % of this variability was within acc...

Research paper thumbnail of Assessment of genetic fidelity among Agave tequilana plants propagated asexually via rhizomes versus in vitro culture

Plant Cell, Tissue and Organ Culture (PCTOC), 2010

Research paper thumbnail of Efficiency of RAPD, ISSR, AFLP and ISTR markers for the detection of polymorphisms and genetic relationships in camote de cerro (Dioscorea spp.)

Electronic Journal of Biotechnology, 2014

Background: At present, species known as camote de cerro (Dioscorea spp.) are found only in the w... more Background: At present, species known as camote de cerro (Dioscorea spp.) are found only in the wilderness in Mexico, but their populations are extremely depleted because they are indiscriminately collected, it is urgent to evaluate the conservation status of these plants in order to design conservation genetics programs. In this study, genetic diversity parameters along with cluster analysis based on Jaccard's coefficient were estimated with the objective to assess the efficiency of Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR), Amplified Fragment Length Polymorphism (AFLP) and Inverse Sequence Tagged Repeat (ISTR) molecular DNA markers in the Dioscorea genus. Results: The polymorphic information contents were quite similar for all markers (≈0.48). Genetic variation of Dioscorea spp., in terms of average heterozygosity was lower with ISTR (0.36), and higher when other markers were used (RAPD = 0.43; ISSR = 0.45 and AFLP = 0.47). Conclusion: This indicates an important level of genetic differences despite the fact that the plant is asexually propagated. Based on the diversity statistics, any marker tested in present work can be recommended for use in large-scale genetic studies of populations. However, the low correlations among different molecular marker systems show the importance of the complementarity of the information that is generated by different markers for genetic studies involving estimation of polymorphism and relationships.

Research paper thumbnail of Evaluación del efecto rizogénico de productos orgánicos en dos tipos de sustrato en esquejes de hortensias (Hydrangea macrophylla)

E-Cucba, Jun 29, 2023

vegetales ornamentales, la hortensia (Hydrangea macrophylla) es una de las más llamativas y popul... more vegetales ornamentales, la hortensia (Hydrangea macrophylla) es una de las más llamativas y populares, debido a su belleza y el tamaño de sus inflorescencias. Su producción comienza regularmente a partir de esquejes terminales que deben enraizarse. En el presente trabajo, se probó la capacidad rizogénica de cuatro productos comerciales a base de reguladores de crecimiento y dos productos orgánicos: parénquima medular de sábila y extracto de algas aplicados en dos sustratos. Los resultados encontrados, mostraron la eficiencia de la sábila para producir callo en los esquejes y mantener la turgencia de los mismos, lo cual implica que ésta pueda ser usada a pequeña y mediana escala en el enraizamiento de esquejes herbáceos como los de la Hydrangea macrophylla.

Research paper thumbnail of Uso en medicina tradicional de Verbesina sphaerocephala A. Gray (Asteraceae) en la comunidad de San Martin de las Flores, Jalisco, México

Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants, Mar 30, 2019

The community of San Martin de las Flores, Jalisco, México has a varied flora, part of which is u... more The community of San Martin de las Flores, Jalisco, México has a varied flora, part of which is used by its residents to treat their diseases. One of these wild species that grows in this community is Verbesina sphaerocephala A.Gray (Asteraceae). The objective of this research was to provide an overview of local knowledge about the traditional uses of this plant. A quantitative ethnobotanical method to analyse the value of traditional use of this species among the community, as well as, bibliographic information on the existing phytochemical and pharmacological properties of species of the genus Verbesina has been used. When examining the general knowledge of V. sphaerocephala among the community, it was concluded that the plant is used mainly in traditional medicine to treat eight different types of diseases, highlighting its use as a wound healing and anti-inflammatory.

Research paper thumbnail of Genetic variability of sargassum liebmannii on the coast of jalisco in the central mexican pacific revealed by molecular markers and morphological traits

Electronic Journal of Biotechnology, Aug 1, 2021

Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwellin... more Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwelling areas in the tropical Mexican Pacific. This brown algae is of great environmental and industrial importance. However, no information is available that documents the genetic or phenotypic variability of the species, which is needed to determine how it may react to environmental variation related to climate change. In this study, S. liebmannii specimens were collected from the coast of Jalisco, Mexico, and a molecular and morphological characterization was conducted. Intraspecific variability was estimated according to the study areas. Results The inter-simple sequence repeat (ISSR) markers indicated a polymorphism percentage of 95%. The Shannon index and Nei index showed relatively low values among the populations (0.3569 and 0.081, respectively). On the other hand, the genetic differentiation coefficient indicated inter- and intrapopulation values of 36.69% and 63.31%, respectively. The Jaccard similarity coefficient was used to determine the degree of similarity among individuals by geographical area. The morphological characteristics and environmental variables that were used to correlate phenotypes and genotypes indicated that S. liebmannii showed low genetic flow due to the presence of geographical barriers due to substrate that was not optimal for algal development. Conclusions The ISSR markers were useful for detecting genetic differences among S. liebmannii individuals. The results indicate that a coupled genotypic-phenotypic study is beneficial for documenting the variation present in little-studied algal species. These studies may be used in future research to clarify taxonomic controversies while generating additional genomic information.

Research paper thumbnail of Application of Seaweed Generates Changes in the Substrate and Stimulates the Growth of Tomato Plants

Plants, Mar 31, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of EFFECT OF LIQUID SEAWEED EXTRACT ON POTTED GROWTH OF Eustoma grandiflorum (Raf.) Shinners)

Tropical and Subtropical Agroecosystems, Jun 10, 2020

Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, im... more Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, improves crop development and consequently yield, since by stimulating natural processes they benefit from the use of nutrients and increases resistance to conditions of biotic and / or abiotic stress. Objective. This investigation evaluated the response of two varieties of Eustoma grandiflorum to foliar and root application of algae extracts, grown in pots under nursery conditions. Methodology. Macroalgae extract was applied in a foliar way (20 mL spray) and directly to the substrate (50 mL), while drinking water was applied as the control. Results. The parameters obtained indicate that the foliar application has noticeably effects on var. Rosie for the variables root length, number of shoots, and number of leaves; while for var. Florida, the variables total plant length and number of shoots obtained greater significance. The method of application via substrate generates positive effects on the total length and stem length for var. Rosie, although the variables with the greatest noticeably were obtained from var. Florida for root length, number of leaves, and flower diameter. Implications. The low doses of the algae extracts, application form and the handling of cultural tasks during the growth of E. grandiflorum, contribute to a better absorption and/or efficiency of the fertilizers in the crop. Conclusion. The application of algae extracts favors the production of E. grandiflorum under nursery conditions, making their use a viable option to minimize the application of conventional fertilizers, thereby attenuating the effects on the environment.

Research paper thumbnail of Evaluación del efecto rizogénico de productos orgánicos en dos tipos de sustrato en esquejes de hortensias (Hydrangea macrophylla)

E-CUCBA

Within the industry for the production of ornamental plant species, the hydrangea (Hydrangea macr... more Within the industry for the production of ornamental plant species, the hydrangea (Hydrangea macrophylla) is one of the moststriking and popular, due to its beauty and the size of its inflorescences. Its production starts regularly from terminal cuttings thatneed to be rooted. In the present work, the rhizogenic capacity of four commercial products based on growth regulators and twoorganic products was tested: aloe medullary parenchyma and algae extract. The results found showed the efficiency of aloe vera toproduce callus in the cuttings and maintain their turgidity, which implies that it can be used on a small and medium scale in therooting of herbaceous cuttings such as those of Hydrangea macrophylla.

Research paper thumbnail of EFFECT OF LIQUID SEAWEED EXTRACT ON POTTED GROWTH OF Eustoma grandiflorum (Raf.) Shinners)

Tropical and Subtropical Agroecosystems

Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, im... more Background. The use of liquid algae extract as a biostimulant when applied to leaves or roots, improves crop development and consequently yield, since by stimulating natural processes they benefit from the use of nutrients and increases resistance to conditions of biotic and / or abiotic stress. Objective. This investigation evaluated the response of two varieties of Eustoma grandiflorum to foliar and root application of algae extracts, grown in pots under nursery conditions. Methodology. Macroalgae extract was applied in a foliar way (20 mL spray) and directly to the substrate (50 mL), while drinking water was applied as the control. Results. The parameters obtained indicate that the foliar application has noticeably effects on var. Rosie for the variables root length, number of shoots, and number of leaves; while for var. Florida, the variables total plant length and number of shoots obtained greater significance. The method of application via substrate generates positive effects ...

Research paper thumbnail of Application of Seaweed Generates Changes in the Substrate and Stimulates the Growth of Tomato Plants

Plants

Ulva ohnoi is a cosmopolitan green seaweed with commercial potential given the biomass that may b... more Ulva ohnoi is a cosmopolitan green seaweed with commercial potential given the biomass that may be generated. We evaluated the effects of substrate changes induced by U. ohnoi application on the vegetative response of tomato plants under greenhouse conditions. First, the decomposition dynamics and N release of the dry seaweed biomass were studied using the litterbag method. Subsequently, we evaluated the effect of seaweed powder (SP) or seaweed extract (SE) applications on substrate and plant growth. Additionally, the growth parameters responses evaluated were related to the changes in substrate properties associated with each treatment. The results showed that the dry seaweed biomass has a rapid rate of degradation (k = 0.07 day−1) and N release (k = 0.024 day−1). The SP application improved the physicochemical and biological characteristics of the substrate by increasing the availability of minerals, the fungi:bacteria ratio, and the growth morphophysiological parameters (length, ...

Research paper thumbnail of Verbesina sphaerocephala andVerbesina fastigiata extracts promote the pot development of Fragaria ananassa

Agroindustrial science, Apr 5, 2022

Esta obra está publicada bajo la licencia CC BY-NC-4.0 Extractos de Verbesina sphaerocephala y Ve... more Esta obra está publicada bajo la licencia CC BY-NC-4.0 Extractos de Verbesina sphaerocephala y Verbesina fastigiata promueven el desarrollo en maceta de Fragaria ananassa Verbesina sphaerocephala and Verbesina fastigiata extracts promote the pot development of Fragaria ananassa

Research paper thumbnail of Presence of arsenic and potentially toxic metals (Cd, Cr, Pb) in water and soil of the NE shore of Chapala Lake, Mexico, and its genotoxic effect in the edible chayote fruit (Sechium edule (Jacq.) Sw.)

European Journal of Horticultural Science, 2020

The presence of potentially toxic elements in waters used for agricultural irrigation is a latent... more The presence of potentially toxic elements in waters used for agricultural irrigation is a latent problem in Lake Chapala, Mexico. Four sites in the polluted area of Chapala lake were studied in this research. Samples of irrigation water and soil were collected from each site in both the dry (DS) and rainy (RS) seasons. Arsenic (As), cadmium (Cd), chromium (Cr), and lead (Pb) were measured in all the samples. Comet assay was used to measure the migration of DNA from the nucleus in order to detect genotoxicity in fruits. ISSR molecular markers were used to assess the detection power of this marker in correspondence with amounts of metals in the samples. The results show a variation of metal concentrations in the DS and RS soil and water samples. The metalloid As in DS water samples exceeded the permissible limits in three of the sampling areas. Cd and Pb exceeded the prescribed limits from collected water in three of the sampling areas in RS. Cd exceeded the maximum permissible limits in soil collected during both DS and RS in the four sampling sites. Data on the concentration of metals shows significant differences between the sampling areas and the control site. Genetic damage (DNA migration from the nuclei) was found in the chayote fruit and the major migration of DNA was associated principally with the presence of As in DS water samples as well as Pb in soil in RS.

Research paper thumbnail of Effects of Nanomaterials/Nanocomposites on Trace Element Uptake and Phytotoxicity

Nanomaterials and Nanocomposites Exposures to Plants

Research paper thumbnail of Estudio preliminar de identificación a nivel molecular, de ecotipos de chile piquín

e-CUCBA

The piquín chili is a plant genetic resource with great potential for culinary nutrition and vita... more The piquín chili is a plant genetic resource with great potential for culinary nutrition and vitamin supply for consumer countriessuch as Mexico. Its study at the molecular level represents a possibility of differentiating the existing ecotypes in each region whereit is produced or where it is consumed. Due to the great morphological variability that it presents, the molecular characterization ofchili as a plant genetic resource represents obtaining the characteristics from the genetic point of view. This is necessary for theidentification of promising genotypes for genetic improvement in addition to documentation of the gene pool. In the present work, apreliminary study was carried out to identify piquín chili ecotypes from nine states of the republic, using the molecular markerISSR (Inter Simple Sequence Repeats). The results obtained provide information on the ability of molecular markers to detectdifferences in ecotypes, which correspond to the different collection regions.

Research paper thumbnail of EFFECT OF DAMINOZIDE ON THE GROWTH AND FLOWERING OF Eustoma grandiflorum PROPAGATED IN POTTED

Acta Scientiarum Polonorum Hortorum Cultus

Daminozide (B-Nine) it is a plant growth regulator, is translocated from the leaves to the stem t... more Daminozide (B-Nine) it is a plant growth regulator, is translocated from the leaves to the stem to avoid elongation and produce more compact plants and uniform growth and vigor. For the cultivation of Lisiathus the information of the damonizide application is scarce despite of great marketing potential, so the objective of this study was to evaluate the effect of daminozide on the vegetative growth and flowering of Lisianthus grown in pot. Granulated Lisianthus seeds were germinated and transplanted into pots under nursery conditions. Three foliar applications of daminozide were realized; two applications in vegetative stage and one in flowering, adding three concentrations in each of them (0.5, 1.0 and 1.5 ml L–1) were used and water was applied as a control during the vegetative and flowering phases. The applications of daminozide at 1.0 and 1.5 ml L–1 were optimal for the vegetative development in Lisianthus (plant height – 15 cm, size of the internodes – 2.5 cm, number of branch...

Research paper thumbnail of The Impact of Aqueous Extracts of Verbesina sphaerocephala and Verbesina fastigiata on Germination and Growth in Solanum lycopersicum and Cucumis sativus Seedlings

Horticulturae

The use of extracts derived from different plants has gained popularity as an alternative option ... more The use of extracts derived from different plants has gained popularity as an alternative option to manage weeds and support phenological development in plants. One of the main problems facing agricultural production is the intensive application of chemical fertilizers that harm the environment. This study investigated the biostimulant effects of the aqueous extracts of Verbesinasphaerocephala and Verbesinafastigiata on the germination and growth of tomato (Solanum lycopersicum) and American cucumber (Cucumis sativus) seedlings. The aqueous extracts of both species of Verbesina were tested at concentrations of 0.5 and 1%. Seed germination and seedling development under nursery conditions were evaluated. The seed germination percentage was reduced in all treatments with the extracts of both Verbesina species when compared to that of the control treatment, which was considered an allelopathic effect. However, seedling growth in pots showed different behavior, with the extracts benefic...

Research paper thumbnail of Uso en Medicina Tradicional De Verbesina Sphaerocephala a Gray Asteraceae en La Comunidad De San Martin De Las Flores Jalisco Mexico

Universidad de Santiago de Chile, Mar 30, 2019

Research paper thumbnail of Genetic variability of Sargassum liebmannii on the coast of Jalisco in the central Mexican Pacific revealed by molecular markers and morphological traits

Electronic Journal of Biotechnology, 2021

Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwellin... more Abstract Background Sargassum liebmannii is widely distributed throughout rocky, coastal upwelling areas in the tropical Mexican Pacific. This brown algae is of great environmental and industrial importance. However, no information is available that documents the genetic or phenotypic variability of the species, which is needed to determine how it may react to environmental variation related to climate change. In this study, S. liebmannii specimens were collected from the coast of Jalisco, Mexico, and a molecular and morphological characterization was conducted. Intraspecific variability was estimated according to the study areas. Results The inter-simple sequence repeat (ISSR) markers indicated a polymorphism percentage of 95%. The Shannon index and Nei index showed relatively low values among the populations (0.3569 and 0.081, respectively). On the other hand, the genetic differentiation coefficient indicated inter- and intrapopulation values of 36.69% and 63.31%, respectively. The Jaccard similarity coefficient was used to determine the degree of similarity among individuals by geographical area. The morphological characteristics and environmental variables that were used to correlate phenotypes and genotypes indicated that S. liebmannii showed low genetic flow due to the presence of geographical barriers due to substrate that was not optimal for algal development. Conclusions The ISSR markers were useful for detecting genetic differences among S. liebmannii individuals. The results indicate that a coupled genotypic-phenotypic study is beneficial for documenting the variation present in little-studied algal species. These studies may be used in future research to clarify taxonomic controversies while generating additional genomic information.

Research paper thumbnail of Verbesina sphaerocephala extracts promote the development of Cucumis sativus and reduce the damage caused by Meloidogyne incognita

Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology, 2021

Uno de los principales problemas fitosanitarios son los nematodos agalladores del género Meloidog... more Uno de los principales problemas fitosanitarios son los nematodos agalladores del género Meloidogyne. El uso de Verbesina sphaerocephala podrían sustituir el uso de nematicidas químicos. El objetivo de esta investigación fue probar si extractos acuosos de V. sphaerocephala promueven el desarrollo y reducen los daños ocasionados por M. incognita en Cucumis sativus. Se evaluaron siete tratamientos en plantas de C. sativus crecidas en macetas en condiciones de invernadero: plantas testigo sin nematodos, plantas con nematodos, con nematodos + carbofuran, con nematodos + Trichoderma, con nematodos + extracto de V. sphaerocephala (concentración al 10, 15 y 20%). Se realizó tres evaluaciones para estimar altura de planta, diámetro de tallo, concentración de clorofila, número de hojas y flores, longitud y peso de raíces, índice de agallamiento, huevos en raíz y larvas en el suelo. Los resultaron mostraron que los extractos de V. sphaerocephala al 10 y 15% promovieron el crecimiento de las p...

Research paper thumbnail of Genetic structure of Zephyranthes fosteri, specie with ornamental and medicinal potential in Mexico

Biotecnia, 2019

Zephyranthes fosteri is a wild species distributed in Mexico, it is popular for its ornamental qu... more Zephyranthes fosteri is a wild species distributed in Mexico, it is popular for its ornamental quality. Anthropogenic activities perturbed its natural habitats and threaten its survival. At present time, there is not enough information about the genetic relationships and structure among Z. fosteri distributed in Mexico. The objective of this investigation was to elucidate the genetic relationships and structure among accessions of Z. fosteri collected in western and southeastern Mexico. ISSR markers were used to establish the genetic variability and genetic structure among 60 accessions randomly collected in western Mexico and one population found in southeastern Mexico. Jaccard’s coefficient and AMOVA analysis determined the similarity and variability among and within accessions, and Bayesian model was used to assess the genetic structure. A mean heterozygosity of 0.49 was found indicated a moderate variability. The AMOVA analysis showed that 75 % of this variability was within acc...

Research paper thumbnail of Assessment of genetic fidelity among Agave tequilana plants propagated asexually via rhizomes versus in vitro culture

Plant Cell, Tissue and Organ Culture (PCTOC), 2010

Research paper thumbnail of Efficiency of RAPD, ISSR, AFLP and ISTR markers for the detection of polymorphisms and genetic relationships in camote de cerro (Dioscorea spp.)

Electronic Journal of Biotechnology, 2014

Background: At present, species known as camote de cerro (Dioscorea spp.) are found only in the w... more Background: At present, species known as camote de cerro (Dioscorea spp.) are found only in the wilderness in Mexico, but their populations are extremely depleted because they are indiscriminately collected, it is urgent to evaluate the conservation status of these plants in order to design conservation genetics programs. In this study, genetic diversity parameters along with cluster analysis based on Jaccard's coefficient were estimated with the objective to assess the efficiency of Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR), Amplified Fragment Length Polymorphism (AFLP) and Inverse Sequence Tagged Repeat (ISTR) molecular DNA markers in the Dioscorea genus. Results: The polymorphic information contents were quite similar for all markers (≈0.48). Genetic variation of Dioscorea spp., in terms of average heterozygosity was lower with ISTR (0.36), and higher when other markers were used (RAPD = 0.43; ISSR = 0.45 and AFLP = 0.47). Conclusion: This indicates an important level of genetic differences despite the fact that the plant is asexually propagated. Based on the diversity statistics, any marker tested in present work can be recommended for use in large-scale genetic studies of populations. However, the low correlations among different molecular marker systems show the importance of the complementarity of the information that is generated by different markers for genetic studies involving estimation of polymorphism and relationships.