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Papers by Anamaria Hosu

Research paper thumbnail of Phytochemicals content, screening and antioxidant/pro-oxidant activities of Carapa procera (barks) (Meliaceae)

South African Journal of Botany

Total phenolic, flavonoid and tannin contents together with antioxidant and pro-oxidant activitie... more Total phenolic, flavonoid and tannin contents together with antioxidant and pro-oxidant activities of Carapa procera were investigated. The antioxidant activity of Carapa procera was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing/ antioxidant power (FRAP) method and 2,2 0-azino bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay. The study of pro-oxidant activity was based on the power producing of iron ion in the Fenton reaction. The total phenolic content (TPC) of the extract was exhibiting high value of 8.2 mg gallic acid equivalents (GAE)/ g of dried Carapa. The study revealed a low percentage of flavonoids 0.04% in the extract and 0.24 mg of tannic acid equivalents (TAE)/g of dried Carapa for the tannin content (TC). Proanthocyanidins were less represented among the tannins 6.80% of the extract. The chromatographic fingerprints showed the presence of tannins and acid, like (+)-catechin, epicatechin, trans-4-hyroxicinnamic, 3,4-dihydroxicinnamic and acid chlorogenic. Carapa procera exhibited high antioxidant activity in the both DPPH and ABTS assays, being 10.45 mg Vitamin C equivalents/g of dried Carapa and 500 mmol Trolox/g of dried Carapa, respectively. The FRAP gave a good result 2.45 mg Vitamin C equivalents /g of dried Carapa. The pro-oxidant activity was 24.25 mg/ml of Carapa extract. The phytochemical screening was the subject of intensive investigations in order to identify the chemical constituents present in medicinal plants, such as Carapa procera and to evaluate their potential biological activities.

Research paper thumbnail of Influence of Intermittent Heating during Maceration on the Antioxidant Capacity of Some Grape Seeds and Skins

Notulae Botanicae Horti Agrobotanici Cluj-napoca, 2010

Ethanolic extracts from seeds and skins of three red grape varieties, namely, 'Cabernet Sauvi... more Ethanolic extracts from seeds and skins of three red grape varieties, namely, 'Cabernet Sauvignon', 'Merlot' and 'Burgund' from a Romanian winery, were prepared by maceration using different temperature conditions. The stable free radicals DPPH (2, 2-diphenyl- 1-picrylhydrazyl) and Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidin-N-oxyl) were used in order to evaluate the antioxidant capacity of the extracts. The variation in time of free radical concentration was followed by double integration of the EPR spectra of the samples obtained after maceration under different conditions (room temperature and intermittent heating). Results showed that the antioxidant capacity depends on the nature of analysed samples (either being seeds or skins) and grape variety. The results also show that the intermittent heating during maceration leads to a decrease of the antioxidant capacity of samples.

Research paper thumbnail of Study Concerning Pesticide Residues in Twenty-seven Romanian White Wines

MIHAI INCEU1, ADELA MEGHESAN1, ION SANDU2,3, ANAMARIA HOSU1, LUOANA FLORENTINA PASCU4, CLAUDIA CI... more MIHAI INCEU1, ADELA MEGHESAN1, ION SANDU2,3, ANAMARIA HOSU1, LUOANA FLORENTINA PASCU4, CLAUDIA CIMPOIU1,* 1 “Babes-Bolyai” University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos, 400028, Cluj-Napoca, Romania 2 Alexandru Ioan Cuza University, ARHEOINVEST Interdisciplinary Platform, Carol I, no. 11, Corp G demisol, 700506, Iasi, Romania 3 Romanian Inventors Forum, 3 Sf. P.Movila Str., L11, III/3, 700089, Iasi, Romania 4 ECOIND Bucharest, 71-73 Drumul Podu Dambovitei Str., 060652, Bucharest, Romania

Research paper thumbnail of High-performance thin-layer chromatography evaluation of the counterfeiting of vanilla flavoring

JPC – Journal of Planar Chromatography – Modern TLC

Research paper thumbnail of Antioxidant content in romanian traditional distilled alcoholic beverages

Reported here is a first detailed analysis of the antioxidant activity in traditional fruit-deriv... more Reported here is a first detailed analysis of the antioxidant activity in traditional fruit-derived distilled alcoholic beverages from Romania (" țuica "). A distinctly highest activity, paralleled by Folin-Ciocalteu data and UV-vis absorbances, was seen in the most common type of such brandy, made of plums; other samples included brandies made of apples, pears, fruit mixtures, wine, and cereals. In fact, the values seen for the plum brandy were, even before maturation with wood, similar to those of commercially-available wood-maturated whisky. Increases of up to one order of magnitude in antioxidant activity were seen in all brandies upon maturation with various types of wood – with mulberry tree by far the most efficient, followed by oak and cherry – and with lowest values seen for acacia. Attempts to identify anticancer activity in concentrated extracts prepared from plum brandy, failed. Copper electron paramagnetic (EPR) signals are shown for the first time in such brandy samples.

Research paper thumbnail of Antioxidant activity prediction and classification of some teas using artificial neural networks

Food Chemistry, 2011

In order to characterise and to classify some teas a simple, rapid and economical method based on... more In order to characterise and to classify some teas a simple, rapid and economical method based on composition, antioxidant activity and artificial neural networks (ANNs) is proposed. For these purpose two types of ANN based applications have been developed: one for predicting the antioxidant activity and a second one for establishing the class of the teas. The complex relationship between the total antioxidant activity (AA) depending on the total flavonoids content (F), total catechins content (C) and total methyl-xanthines content (MX) of commercial teas was revealed by the first designed feed-forward ANN. Secondly, using a probabilistic ANN, successful tea classification in various classes (green tea, black tea and express black tea) was also performed.

Research paper thumbnail of ICP-MS characterization of some Roumanian white wines by their mineral content

Research paper thumbnail of Multiresidue analysis of pesticides and metabolites from fruits and vegetables by gas chromatography-time-of-flight mass spectrometry

Acta Chromatographica, 2015

This work describes the comparison of five sample extraction methods for the determination of pes... more This work describes the comparison of five sample extraction methods for the determination of pesticide residues in grapes and vegetables by using gas chromatography—time-of-flight mass spectrometry (GC—TOF-MS). These methods were based on original methods QuEChERS, mini-Luke, ethyl acetate, and DIN EN 15637, and some of these were slightly modified to increase the number of identified and quantified pesticides, to improve their quantification limits, and to be fast and less expensive in terms of material cost. The acceptable performance parameters combined with the properties of easy and quick handling and cost-effectiveness have made mini-Luke modified extraction method as the most favorable in the pesticide residues analysis from grapes and tomatoes by GC—TOF-MS. The efficiency of the chosen extraction method was also verified for lettuce; for this matrix, a cleanup step with graphitized black carbon (GBC) was added. Analysis of extracts was carried out by GC—TOF-MS within 29.2 min run time. The GC met...

Research paper thumbnail of Evaluation of various biological activities of natural compounds by TLC/HPTLC

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of Thin-layer chromatography in spices analysis

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of The Influence of in Vitro Gastrointestinal Digestion of Brassica oleracea Florets on the Antioxidant Activity and Chlorophyll, Carotenoid and Phenolic Content

Antioxidants

Brassica oleracea L. var. Italica is known to contain a wide variety of antioxidants and due to t... more Brassica oleracea L. var. Italica is known to contain a wide variety of antioxidants and due to the protection against various diseases its consumption has been increasing over the years. Thus, knowledge of the changes that occur during the digestion process is of great interest. The aim of this study was to investigate the influence of in vitro gastrointestinal digestion of broccoli on antioxidant activity and on the chlorophyll, carotenoid and phenolic content. First, the ultrasound-assisted extraction of bioactive compounds was optimized and the kinetic model was evaluated. Then, the broccoli was subjected to a static simulated digestion. The antioxidant activity was monitored by ABTS [2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate)] assay and the contents of target compounds were investigated by UV-Vis spectrophotometry and thin-layer chromatography. The optimum conditions were: solvent—ethanol; time—20 min and temperature—30 °C, and a second order kinetic model was found to ...

Research paper thumbnail of Identification and quantification of some pesticide metabolites from vegetables by GC-TOF-MS and LC-MS-QQQ

Studia Universitatis Babeș-Bolyai Chemia

Considering the international situation regarding the residues of pesticides in vegetables and fr... more Considering the international situation regarding the residues of pesticides in vegetables and fruits and the requirement for sensitive and reliable analytical methods that are able to ensure the compliance of marketed food commodities with the law on food safety, the aim of this paper was the identification and determination of some degradation products and metabolites of ten most often used pesticides. Two analytical approaches are proposed and used for the development of reliable screening, quantification and confirmation of pesticides metabolites in different vegetables. The proposed methods have different approaches than the other multi-residue methods for vegetables allowing the extensive investigation of previously undetectable or unknown pesticide metabolites in vegetables. These methods were tested on cherry tomato, cucumber, and chili pepper, some of the most widely consumed vegetables. The results suggests that spraying pesticides in high doses lead to high levels of pesticide residues in the case of some studied vegetables and the concentration of metabolites together concentration of parent pesticide must be taken into account in order to establish the MRL's in vegetables and fruits.

Research paper thumbnail of Trends in analysis of vegetables by high performance TLC

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of Chemo-mapping and biochemical-modulatory and antioxidant/prooxidant effect of Galium verum extract during acute restraint and dark stress in female rats

PloS one, 2018

Galium verum is a well-known medicinal plant which is used in various pathologies. G. verum extra... more Galium verum is a well-known medicinal plant which is used in various pathologies. G. verum extracts are characterized here using chromatography, where among the rich pool of phenolic acids of flavonoids two known anti-stress modulators, chlorogenic acid and rutin are identified in high quantities. Additionally, the extracts are characterized using a series of in vitro assays (EPR, DPPH, TPC and TEAC). Considering the chemical findings, the potential beneficial effects of the G. verum extract are explored here in a living organism exposed to stress induced oxidative damages. Thus, the biochemical-modulatory and antioxidant roles of two doses of G. verum extract are examined in animals exposed to acute restraint and dark stress (S). The animals were divided in groups [control, S, SG1 (exposed to 25 mg G. verum extract), SG2 (50 mg extract)]. Increased levels of lipid peroxidation (TBARS from 4.43 to 8.06 nmol/mL), corticosterone from 0.43 to 1.96 μg/dL and epinephrine from 44.43 to 1...

Research paper thumbnail of Thin-layer chromatography applied in quality assessment of beverages derived from fruits

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of Study of the Antioxidant Property Variation of Cornelian Cherry Fruits during Storage Using HPTLC and Spectrophotometric Assays

Journal of Analytical Methods in Chemistry, 2016

Cornus species fruits are well known as a rich source of antioxidant compounds responsible for th... more Cornus species fruits are well known as a rich source of antioxidant compounds responsible for their diverse health benefits. The present study aims to investigate the variation of the total antioxidant capacity of Cornelian cherry (Cornus masL.) fruits during storage, using high-performance thin-layer chromatography (HPTLC) and two spectrophotometric assays based on different mechanisms: the 2,2-azinobis(3-ethylbenzothiazolyne-6-sulphonic acid) radical cation (ABTS+∙) assay and the ferric reducing antioxidant power (FRAP) assay. The fruit extract was stored at room temperature (22°C) for 19 days. No major differences in the total antioxidant capacity were observed during this period, indicating that storage does not have any deleterious effect on the antioxidant properties of the investigated fruits extract. The antioxidant capacity varied between 12.91 and 12.83 µmol Trolox/g fruit as determined by the HPTLC method and from 36.13 to 33.93 µmol Trolox/g fruit as determined by the A...

Research paper thumbnail of The Influence of the Variety, Vineyard, and Vintage on the Romanian White Wines Quality

Journal of Analytical Methods in Chemistry, 2016

The wine is one of the most consumed drinks over the world, being subjected to falsification or a... more The wine is one of the most consumed drinks over the world, being subjected to falsification or adulteration regarding the variety, vintage, and geographical region. In this study, the influence of different characteristics of wines (type, production year, and origin) on the total phenolic content, total flavonoids content, antioxidant activity, total sugars content, pH, and18O/16O isotopic ratio was investigated. The differentiation of selected wines on the basis of tested parameters was investigated using chemometric techniques, such as analysis of variance, cluster analysis, and principal component analysis. The experimental results are in agreement with other outcomes and allow concluding that variety and vineyard have the major influence on the studied parameters, but, also, statistical interaction effect between year and vineyard and year and variety is observed in some cases. The obtained results have demonstrated that these parameters together with chemometric techniques sho...

Research paper thumbnail of Comparative evaluation of antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) methods

JPC - Journal of Planar Chromatography - Modern TLC, 2016

Research paper thumbnail of Total phenolics, anthocyanins, antioxidant and pro-oxidant activity of some red fruits teas

Acta Chimica Slovenica, 2016

Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable ... more Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable health benefits, many functional foods of fruit origin, including fruit teas, are present on the market and there is an increased interest regarding the investigation of their nutritional parameters and quality. The aims of our study were: 1) to determine the total phenolic content (TPC), total anthocyanins content (TAC), antioxidant activity (AA), the scavenging capacity (IC50), the pro-oxidant activity (Pro-ox) and Pro-Antidex of 12 commercially available red fruit teas, 2) to classify the analysed teas and 3) to evaluate the similarities between samples. The TPC was between 12.5 and 29.3 mg gallic acid equivalents (GAE)/g tea, the TAC varied between 2.6 and 5.6mg cyanidin-3-glucoside (Cy-3-glu)/g tea and AA was in the range of 10.9-19.1 mg ascorbic acid equivalents (AAE)/g tea. The Pro-ox activity varied between 3.9 and 10.0 mg/mL tea extract and Pro-Antidex was between 3.3 and 7.3.

Research paper thumbnail of Total Phenolics, Total Anthocyanins, Antioxidant and Pro-oxidant Activity of Some Red Fruits Teas

Acta chimica Slovenica, 2016

Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable ... more Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable health benefits, many functional foods of fruit origin, including fruit teas, are present on the market and there is an increased interest regarding the investigation of their nutritional parameters and quality. The aims of our study were: 1) to determine the total phenolic content (TPC), total anthocyanins content (TAC), antioxidant activity (AA), the scavenging capacity (IC50), the pro-oxidant activity (Pro-ox) and Pro-Antidex of 12 commercially available red fruit teas, 2) to classify the analysed teas and 3) to evaluate the similarities between samples. The TPC was between 12.5 and 29.3 mg gallic acid equivalents (GAE)/g tea, the TAC varied between 2.6 and 5.6 mg cyanidin-3-glucoside (Cy-3-glu)/g tea and AA was in the range of 10.9-19.1 mg ascorbic acid equivalents (AAE)/g tea. The Pro-ox activity varied between 3.9 and 10.0 mg/mL tea extract and Pro-Antidex was between 3.3 and 7.3.

Research paper thumbnail of Phytochemicals content, screening and antioxidant/pro-oxidant activities of Carapa procera (barks) (Meliaceae)

South African Journal of Botany

Total phenolic, flavonoid and tannin contents together with antioxidant and pro-oxidant activitie... more Total phenolic, flavonoid and tannin contents together with antioxidant and pro-oxidant activities of Carapa procera were investigated. The antioxidant activity of Carapa procera was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing/ antioxidant power (FRAP) method and 2,2 0-azino bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay. The study of pro-oxidant activity was based on the power producing of iron ion in the Fenton reaction. The total phenolic content (TPC) of the extract was exhibiting high value of 8.2 mg gallic acid equivalents (GAE)/ g of dried Carapa. The study revealed a low percentage of flavonoids 0.04% in the extract and 0.24 mg of tannic acid equivalents (TAE)/g of dried Carapa for the tannin content (TC). Proanthocyanidins were less represented among the tannins 6.80% of the extract. The chromatographic fingerprints showed the presence of tannins and acid, like (+)-catechin, epicatechin, trans-4-hyroxicinnamic, 3,4-dihydroxicinnamic and acid chlorogenic. Carapa procera exhibited high antioxidant activity in the both DPPH and ABTS assays, being 10.45 mg Vitamin C equivalents/g of dried Carapa and 500 mmol Trolox/g of dried Carapa, respectively. The FRAP gave a good result 2.45 mg Vitamin C equivalents /g of dried Carapa. The pro-oxidant activity was 24.25 mg/ml of Carapa extract. The phytochemical screening was the subject of intensive investigations in order to identify the chemical constituents present in medicinal plants, such as Carapa procera and to evaluate their potential biological activities.

Research paper thumbnail of Influence of Intermittent Heating during Maceration on the Antioxidant Capacity of Some Grape Seeds and Skins

Notulae Botanicae Horti Agrobotanici Cluj-napoca, 2010

Ethanolic extracts from seeds and skins of three red grape varieties, namely, 'Cabernet Sauvi... more Ethanolic extracts from seeds and skins of three red grape varieties, namely, 'Cabernet Sauvignon', 'Merlot' and 'Burgund' from a Romanian winery, were prepared by maceration using different temperature conditions. The stable free radicals DPPH (2, 2-diphenyl- 1-picrylhydrazyl) and Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidin-N-oxyl) were used in order to evaluate the antioxidant capacity of the extracts. The variation in time of free radical concentration was followed by double integration of the EPR spectra of the samples obtained after maceration under different conditions (room temperature and intermittent heating). Results showed that the antioxidant capacity depends on the nature of analysed samples (either being seeds or skins) and grape variety. The results also show that the intermittent heating during maceration leads to a decrease of the antioxidant capacity of samples.

Research paper thumbnail of Study Concerning Pesticide Residues in Twenty-seven Romanian White Wines

MIHAI INCEU1, ADELA MEGHESAN1, ION SANDU2,3, ANAMARIA HOSU1, LUOANA FLORENTINA PASCU4, CLAUDIA CI... more MIHAI INCEU1, ADELA MEGHESAN1, ION SANDU2,3, ANAMARIA HOSU1, LUOANA FLORENTINA PASCU4, CLAUDIA CIMPOIU1,* 1 “Babes-Bolyai” University, Faculty of Chemistry and Chemical Engineering, 11 Arany Janos, 400028, Cluj-Napoca, Romania 2 Alexandru Ioan Cuza University, ARHEOINVEST Interdisciplinary Platform, Carol I, no. 11, Corp G demisol, 700506, Iasi, Romania 3 Romanian Inventors Forum, 3 Sf. P.Movila Str., L11, III/3, 700089, Iasi, Romania 4 ECOIND Bucharest, 71-73 Drumul Podu Dambovitei Str., 060652, Bucharest, Romania

Research paper thumbnail of High-performance thin-layer chromatography evaluation of the counterfeiting of vanilla flavoring

JPC – Journal of Planar Chromatography – Modern TLC

Research paper thumbnail of Antioxidant content in romanian traditional distilled alcoholic beverages

Reported here is a first detailed analysis of the antioxidant activity in traditional fruit-deriv... more Reported here is a first detailed analysis of the antioxidant activity in traditional fruit-derived distilled alcoholic beverages from Romania (" țuica "). A distinctly highest activity, paralleled by Folin-Ciocalteu data and UV-vis absorbances, was seen in the most common type of such brandy, made of plums; other samples included brandies made of apples, pears, fruit mixtures, wine, and cereals. In fact, the values seen for the plum brandy were, even before maturation with wood, similar to those of commercially-available wood-maturated whisky. Increases of up to one order of magnitude in antioxidant activity were seen in all brandies upon maturation with various types of wood – with mulberry tree by far the most efficient, followed by oak and cherry – and with lowest values seen for acacia. Attempts to identify anticancer activity in concentrated extracts prepared from plum brandy, failed. Copper electron paramagnetic (EPR) signals are shown for the first time in such brandy samples.

Research paper thumbnail of Antioxidant activity prediction and classification of some teas using artificial neural networks

Food Chemistry, 2011

In order to characterise and to classify some teas a simple, rapid and economical method based on... more In order to characterise and to classify some teas a simple, rapid and economical method based on composition, antioxidant activity and artificial neural networks (ANNs) is proposed. For these purpose two types of ANN based applications have been developed: one for predicting the antioxidant activity and a second one for establishing the class of the teas. The complex relationship between the total antioxidant activity (AA) depending on the total flavonoids content (F), total catechins content (C) and total methyl-xanthines content (MX) of commercial teas was revealed by the first designed feed-forward ANN. Secondly, using a probabilistic ANN, successful tea classification in various classes (green tea, black tea and express black tea) was also performed.

Research paper thumbnail of ICP-MS characterization of some Roumanian white wines by their mineral content

Research paper thumbnail of Multiresidue analysis of pesticides and metabolites from fruits and vegetables by gas chromatography-time-of-flight mass spectrometry

Acta Chromatographica, 2015

This work describes the comparison of five sample extraction methods for the determination of pes... more This work describes the comparison of five sample extraction methods for the determination of pesticide residues in grapes and vegetables by using gas chromatography—time-of-flight mass spectrometry (GC—TOF-MS). These methods were based on original methods QuEChERS, mini-Luke, ethyl acetate, and DIN EN 15637, and some of these were slightly modified to increase the number of identified and quantified pesticides, to improve their quantification limits, and to be fast and less expensive in terms of material cost. The acceptable performance parameters combined with the properties of easy and quick handling and cost-effectiveness have made mini-Luke modified extraction method as the most favorable in the pesticide residues analysis from grapes and tomatoes by GC—TOF-MS. The efficiency of the chosen extraction method was also verified for lettuce; for this matrix, a cleanup step with graphitized black carbon (GBC) was added. Analysis of extracts was carried out by GC—TOF-MS within 29.2 min run time. The GC met...

Research paper thumbnail of Evaluation of various biological activities of natural compounds by TLC/HPTLC

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of Thin-layer chromatography in spices analysis

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of The Influence of in Vitro Gastrointestinal Digestion of Brassica oleracea Florets on the Antioxidant Activity and Chlorophyll, Carotenoid and Phenolic Content

Antioxidants

Brassica oleracea L. var. Italica is known to contain a wide variety of antioxidants and due to t... more Brassica oleracea L. var. Italica is known to contain a wide variety of antioxidants and due to the protection against various diseases its consumption has been increasing over the years. Thus, knowledge of the changes that occur during the digestion process is of great interest. The aim of this study was to investigate the influence of in vitro gastrointestinal digestion of broccoli on antioxidant activity and on the chlorophyll, carotenoid and phenolic content. First, the ultrasound-assisted extraction of bioactive compounds was optimized and the kinetic model was evaluated. Then, the broccoli was subjected to a static simulated digestion. The antioxidant activity was monitored by ABTS [2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate)] assay and the contents of target compounds were investigated by UV-Vis spectrophotometry and thin-layer chromatography. The optimum conditions were: solvent—ethanol; time—20 min and temperature—30 °C, and a second order kinetic model was found to ...

Research paper thumbnail of Identification and quantification of some pesticide metabolites from vegetables by GC-TOF-MS and LC-MS-QQQ

Studia Universitatis Babeș-Bolyai Chemia

Considering the international situation regarding the residues of pesticides in vegetables and fr... more Considering the international situation regarding the residues of pesticides in vegetables and fruits and the requirement for sensitive and reliable analytical methods that are able to ensure the compliance of marketed food commodities with the law on food safety, the aim of this paper was the identification and determination of some degradation products and metabolites of ten most often used pesticides. Two analytical approaches are proposed and used for the development of reliable screening, quantification and confirmation of pesticides metabolites in different vegetables. The proposed methods have different approaches than the other multi-residue methods for vegetables allowing the extensive investigation of previously undetectable or unknown pesticide metabolites in vegetables. These methods were tested on cherry tomato, cucumber, and chili pepper, some of the most widely consumed vegetables. The results suggests that spraying pesticides in high doses lead to high levels of pesticide residues in the case of some studied vegetables and the concentration of metabolites together concentration of parent pesticide must be taken into account in order to establish the MRL's in vegetables and fruits.

Research paper thumbnail of Trends in analysis of vegetables by high performance TLC

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of Chemo-mapping and biochemical-modulatory and antioxidant/prooxidant effect of Galium verum extract during acute restraint and dark stress in female rats

PloS one, 2018

Galium verum is a well-known medicinal plant which is used in various pathologies. G. verum extra... more Galium verum is a well-known medicinal plant which is used in various pathologies. G. verum extracts are characterized here using chromatography, where among the rich pool of phenolic acids of flavonoids two known anti-stress modulators, chlorogenic acid and rutin are identified in high quantities. Additionally, the extracts are characterized using a series of in vitro assays (EPR, DPPH, TPC and TEAC). Considering the chemical findings, the potential beneficial effects of the G. verum extract are explored here in a living organism exposed to stress induced oxidative damages. Thus, the biochemical-modulatory and antioxidant roles of two doses of G. verum extract are examined in animals exposed to acute restraint and dark stress (S). The animals were divided in groups [control, S, SG1 (exposed to 25 mg G. verum extract), SG2 (50 mg extract)]. Increased levels of lipid peroxidation (TBARS from 4.43 to 8.06 nmol/mL), corticosterone from 0.43 to 1.96 μg/dL and epinephrine from 44.43 to 1...

Research paper thumbnail of Thin-layer chromatography applied in quality assessment of beverages derived from fruits

Journal of Liquid Chromatography & Related Technologies

Research paper thumbnail of Study of the Antioxidant Property Variation of Cornelian Cherry Fruits during Storage Using HPTLC and Spectrophotometric Assays

Journal of Analytical Methods in Chemistry, 2016

Cornus species fruits are well known as a rich source of antioxidant compounds responsible for th... more Cornus species fruits are well known as a rich source of antioxidant compounds responsible for their diverse health benefits. The present study aims to investigate the variation of the total antioxidant capacity of Cornelian cherry (Cornus masL.) fruits during storage, using high-performance thin-layer chromatography (HPTLC) and two spectrophotometric assays based on different mechanisms: the 2,2-azinobis(3-ethylbenzothiazolyne-6-sulphonic acid) radical cation (ABTS+∙) assay and the ferric reducing antioxidant power (FRAP) assay. The fruit extract was stored at room temperature (22°C) for 19 days. No major differences in the total antioxidant capacity were observed during this period, indicating that storage does not have any deleterious effect on the antioxidant properties of the investigated fruits extract. The antioxidant capacity varied between 12.91 and 12.83 µmol Trolox/g fruit as determined by the HPTLC method and from 36.13 to 33.93 µmol Trolox/g fruit as determined by the A...

Research paper thumbnail of The Influence of the Variety, Vineyard, and Vintage on the Romanian White Wines Quality

Journal of Analytical Methods in Chemistry, 2016

The wine is one of the most consumed drinks over the world, being subjected to falsification or a... more The wine is one of the most consumed drinks over the world, being subjected to falsification or adulteration regarding the variety, vintage, and geographical region. In this study, the influence of different characteristics of wines (type, production year, and origin) on the total phenolic content, total flavonoids content, antioxidant activity, total sugars content, pH, and18O/16O isotopic ratio was investigated. The differentiation of selected wines on the basis of tested parameters was investigated using chemometric techniques, such as analysis of variance, cluster analysis, and principal component analysis. The experimental results are in agreement with other outcomes and allow concluding that variety and vineyard have the major influence on the studied parameters, but, also, statistical interaction effect between year and vineyard and year and variety is observed in some cases. The obtained results have demonstrated that these parameters together with chemometric techniques sho...

Research paper thumbnail of Comparative evaluation of antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) methods

JPC - Journal of Planar Chromatography - Modern TLC, 2016

Research paper thumbnail of Total phenolics, anthocyanins, antioxidant and pro-oxidant activity of some red fruits teas

Acta Chimica Slovenica, 2016

Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable ... more Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable health benefits, many functional foods of fruit origin, including fruit teas, are present on the market and there is an increased interest regarding the investigation of their nutritional parameters and quality. The aims of our study were: 1) to determine the total phenolic content (TPC), total anthocyanins content (TAC), antioxidant activity (AA), the scavenging capacity (IC50), the pro-oxidant activity (Pro-ox) and Pro-Antidex of 12 commercially available red fruit teas, 2) to classify the analysed teas and 3) to evaluate the similarities between samples. The TPC was between 12.5 and 29.3 mg gallic acid equivalents (GAE)/g tea, the TAC varied between 2.6 and 5.6mg cyanidin-3-glucoside (Cy-3-glu)/g tea and AA was in the range of 10.9-19.1 mg ascorbic acid equivalents (AAE)/g tea. The Pro-ox activity varied between 3.9 and 10.0 mg/mL tea extract and Pro-Antidex was between 3.3 and 7.3.

Research paper thumbnail of Total Phenolics, Total Anthocyanins, Antioxidant and Pro-oxidant Activity of Some Red Fruits Teas

Acta chimica Slovenica, 2016

Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable ... more Fruits represent one of the main dietary sources of bioactive compounds. Due to their remarkable health benefits, many functional foods of fruit origin, including fruit teas, are present on the market and there is an increased interest regarding the investigation of their nutritional parameters and quality. The aims of our study were: 1) to determine the total phenolic content (TPC), total anthocyanins content (TAC), antioxidant activity (AA), the scavenging capacity (IC50), the pro-oxidant activity (Pro-ox) and Pro-Antidex of 12 commercially available red fruit teas, 2) to classify the analysed teas and 3) to evaluate the similarities between samples. The TPC was between 12.5 and 29.3 mg gallic acid equivalents (GAE)/g tea, the TAC varied between 2.6 and 5.6 mg cyanidin-3-glucoside (Cy-3-glu)/g tea and AA was in the range of 10.9-19.1 mg ascorbic acid equivalents (AAE)/g tea. The Pro-ox activity varied between 3.9 and 10.0 mg/mL tea extract and Pro-Antidex was between 3.3 and 7.3.