Anas Haruna - Academia.edu (original) (raw)

Papers by Anas Haruna

Research paper thumbnail of The use of Traditional Medicines for Sexual Enhancement in Northern Nigeria

Journal of Community Health

Research paper thumbnail of Factors associated with acceptance of COVID-19 vaccine among University health sciences students in Northwest Nigeria

PLOS ONE, 2021

Students of the health sciences are the future frontliners to fight pandemics. The students’ part... more Students of the health sciences are the future frontliners to fight pandemics. The students’ participation in COVID-19 response varies across countries and are mostly for educational purposes. Understanding the determinants of COVID-19 vaccine acceptability is necessary for a successful vaccination program. This study aimed to investigate the factors associated with COVID-19 vaccine acceptance among health sciences students in Northwest Nigeria. The study was an online self-administered cross-sectional study involving a survey among students of health sciences in some selected universities in Northwest Nigeria. The survey collected pertinent data from the students, including socio-demographic characteristics, risk perception for COVID-19, and willingness to accept the COVID-19 vaccine. Multiple logistic regression was used to determine the predictors of COVID-19 vaccine acceptance. A total of 440 responses with a median (interquartile range) age of 23 (4.0) years were included in th...

Research paper thumbnail of Betulin, a Newly Characterized Compound in Acacia Auriculiformis Bark, is a Multi-target Protein Kinase Inhibitor

The purpose of this work is to investigate the protein kinase inhibitory activity of constituents... more The purpose of this work is to investigate the protein kinase inhibitory activity of constituents from ethyl acetate soluble fraction of Acacia auriculiformis stem bark. Column chromatography, gel filtration and NMR spectroscopy were used to purified and characterized betulin from the extract. Betulin which is a known inducer of apoptosis was screened against a panel of 16 disease-related protein kinases. Betulin was shown to inhibit Abelson murine leukemia viral oncogene homolog 1 (ABL1) kinase, casein kinase 1epsilon (CK1epsilon), glycogen synthase kinase 3alpha/β (GSK-3alpha/β), Janus kinase 3 (JAK3), NIMA Related Kinase 6 (NEK6) and vascular endothelial growth factor receptor 2 kinase (VEGFR2) and with activity in µM range. The effect of betulin on the cell viability of doxorubicin-resistant K562R chronic myelogenous leukemia cells was then verified to underline its putative use as anti-cancer compound. Betulin was shown to modulate the mitogen-activated protein (MAP) kinase pat...

Research paper thumbnail of Participatory Evaluation of Drought Tolerant Maize Varieties using Mother-Baby Trial Model: A Case Study in the Forest-Savannah Transition Zone of Ghana

Journal of Agronomy, 2011

Research paper thumbnail of COVID-19 vaccine acceptability among health sciences students in Northwest Nigeria

Anonymized survey dataset of COVID-19 vaccine acceptability among health sciences students in Nor... more Anonymized survey dataset of COVID-19 vaccine acceptability among health sciences students in Northwest Nigeria

Research paper thumbnail of Farmers Perception for Effective Extension Teaching Methods in Katcha and Bida LGAs of Niger State, Nigeria

The study assessed farmers’ perception for effective extension teaching methods in Niger State. T... more The study assessed farmers’ perception for effective extension teaching methods in Niger State. The specific objectives were to describe the socioeconomic characteristics of farmers in the study area, identify the extension teaching methods used by extension agents in and examine the farmers’ perception of effectiveness of the extension teaching methods. Multi-stage sampling technique was used for this study. A total of 114 respondents were randomly selected from Badeggi, and Katcha in Katcha LGA, Dangana and Bida in Bida LGA. Data was collected with the aid of structured questionnaire. Descriptive statistics such as frequency and percentages and Mean scores was used to analyze objectives. The majority of the farmers (39.5%) were between ages of 20-29. The mean age was 31 years. The findings also showed that the majority (60.5%) of the respondents were male. The majority (50%) of the farmers were married, most (42.1%) had farming experience of 1-10 with a mean of 12 years. Results ...

Research paper thumbnail of Helminth Parasites of Goats and Sheep at Slaughter House in Lafia, Nasarawa State, Nigeria

FUDMA JOURNAL OF SCIENCES

Helminth parasites have been recognized as one of the major constraints to both goat and sheep pr... more Helminth parasites have been recognized as one of the major constraints to both goat and sheep production in Nigeria and other developing countries. A study aimed to determine helminth parasites of goats and sheep at slaughter in Lafia from June to July 2019. Two hundred fecal samples were collected and analyzed from 100 goats and 100 sheep using the direct smear method. The fecal sample examined revealed an overall prevalence of 155(77.5%), with goat having 80(80.0%), sheep 75(75.0%). Thirteen helminth parasites were found with nematode the most prevalent 103(66.5%), trematode had 35(22.6%), and cestode the least with 17(10.1%) and there was a statistically significant difference among the parasites (P<0.001). According to sex, females 59(43.7%) were more infected than males 25(38.5%) and there was a statistically significant difference (P<0.001). Age of sampled animals (Goat and Sheep) shows that <1 year had 28(18.1%), younger animals within the ages of <2 years 65(41....

Research paper thumbnail of Participatory Evaluation of Drought Tolerant Maize Varieties in the Guinea Savanna of Ghana Using Mother and Baby Trial Design

Maize (Zea mays) is a major food crop in Ghana but grain yields are low as a result of drought an... more Maize (Zea mays) is a major food crop in Ghana but grain yields are low as a result of drought and low soil fertility. This study evaluated drought tolerant maize varieties in 2008 and 2009 in the Guinea savanna of Ghana using researcher-managed mother and farmer-managed baby trial design. Mean grain yields ranged between 2574 and 3462 kg/ha for the mother trials and 1460 and 2328 kg/ha for baby trials. Several improved varieties performed better than the best local varieties, but two preferred varieties, TZEE Y POP STR QPM C0 and EVDT W 99 STR QPM CO which produced 35-52% more grain than the best local varieties of similar maturity rating were released in 2010. Farmers have multiple criteria for evaluating maize varieties apart from yield, though yield, larger cob and grain size were the three key criteria used by farmers to select and rank varieties. Researchers should incorporate farmers' preferences in selecting varieties in the breeding process in order to increase likelihood of adoption of the varieties. the productivity of maize-based cropping systems could increase and stabilize rural incomes, alleviate poverty and reduce food insecurity in this region (Kamara et al., 2006). Through collaborative efforts between the International Institute of Tropical Agriculture (IITA) and national maize improvement programmes in WCA, several maize varieties that are either drought tolerant (DT) or mature earlier to escape drought have been developed and evaluated under researcher-managed conditions

Research paper thumbnail of Tetrahydroxy Flavone from Acacia auriculiformis A. Cunn Ex Benth. (Fabaceae) with Novel Kinase Activity

Pharmacognosy Journal

Background: The decoctions of the bark of Acacia auriculiformis are used in folkloric medicine to... more Background: The decoctions of the bark of Acacia auriculiformis are used in folkloric medicine to relieve pain and inflammation and as remedy for cancer. Objective: The aim of this work is to screen the extract and fractions of Acacia auriculiformis for protein kinase inhibitory activity and also to isolate and characterize chemical entities from this plant and evaluate their protein kinase inhibitory activity. Materials and Methods: Kinase inhibitory activity were assayed in appropriate buffer, with either protein or peptide as substrate in the presence of 15µM (33-P) ATP (3,000Ci/mmol; 10mCi/ml) in a final volume of 30µL. Controls were performed with appropriate dilutions of dimethyl sulphoxide. A portion of the Chloroform extract, ethylacetate and n-butanol soluble fractions of the stem bark of Acacia auriculiformis were screened against a panel of disease-related protein kinases and the active fractions was tested over a wide range of concentrations from 0.016 to 50µg/ml and the IC 50 values were determined from the dose response curve. The most active fraction was subjected to chromatographic separation using Silica gel G column chromatography and sephadex LH-20 to give compound I. The structure of the isolated compound was elucidated using NMR and LC-MS. Results: The Primary screening of the extract and fractions showed that the chloroform extract was inactive against all the protein kinases investigated, while the ethylacetate and n-butanol soluble fractions inhibited all the protein kinases tested. Compound I also inhibited all the kinases tested. The IC 50 of the active fractions and compound were also evaluated. Ethylacetate fraction inhibited all the kinases tested with the highest activity against Haspine kinase with IC 50 of 1.0 µg/ml, while n-butanol also gave the highest activity against Haspine kinase with 1C 50 of 1.3 µg/ml. From the active ethylacetate fractions 3, 4', 7, 8-tetrahydroxy flavone was isolated. The Compound exhibited the maximal activity against DYRK1A kinase with an IC 50 of 2.05 µg/ml followed by CDK9 with an IC 50 of 2.28 µg/ml. Conclusion: 3, 4',7, 8-tetrahydroxy flavone was isolated was found to be a DYRK1A and CDK9 inhibitor which might justify the anticancer potential of this plant.

Research paper thumbnail of The use of Traditional Medicines for Sexual Enhancement in Northern Nigeria

Journal of Community Health

Research paper thumbnail of Factors associated with acceptance of COVID-19 vaccine among University health sciences students in Northwest Nigeria

PLOS ONE, 2021

Students of the health sciences are the future frontliners to fight pandemics. The students’ part... more Students of the health sciences are the future frontliners to fight pandemics. The students’ participation in COVID-19 response varies across countries and are mostly for educational purposes. Understanding the determinants of COVID-19 vaccine acceptability is necessary for a successful vaccination program. This study aimed to investigate the factors associated with COVID-19 vaccine acceptance among health sciences students in Northwest Nigeria. The study was an online self-administered cross-sectional study involving a survey among students of health sciences in some selected universities in Northwest Nigeria. The survey collected pertinent data from the students, including socio-demographic characteristics, risk perception for COVID-19, and willingness to accept the COVID-19 vaccine. Multiple logistic regression was used to determine the predictors of COVID-19 vaccine acceptance. A total of 440 responses with a median (interquartile range) age of 23 (4.0) years were included in th...

Research paper thumbnail of Betulin, a Newly Characterized Compound in Acacia Auriculiformis Bark, is a Multi-target Protein Kinase Inhibitor

The purpose of this work is to investigate the protein kinase inhibitory activity of constituents... more The purpose of this work is to investigate the protein kinase inhibitory activity of constituents from ethyl acetate soluble fraction of Acacia auriculiformis stem bark. Column chromatography, gel filtration and NMR spectroscopy were used to purified and characterized betulin from the extract. Betulin which is a known inducer of apoptosis was screened against a panel of 16 disease-related protein kinases. Betulin was shown to inhibit Abelson murine leukemia viral oncogene homolog 1 (ABL1) kinase, casein kinase 1epsilon (CK1epsilon), glycogen synthase kinase 3alpha/β (GSK-3alpha/β), Janus kinase 3 (JAK3), NIMA Related Kinase 6 (NEK6) and vascular endothelial growth factor receptor 2 kinase (VEGFR2) and with activity in µM range. The effect of betulin on the cell viability of doxorubicin-resistant K562R chronic myelogenous leukemia cells was then verified to underline its putative use as anti-cancer compound. Betulin was shown to modulate the mitogen-activated protein (MAP) kinase pat...

Research paper thumbnail of Participatory Evaluation of Drought Tolerant Maize Varieties using Mother-Baby Trial Model: A Case Study in the Forest-Savannah Transition Zone of Ghana

Journal of Agronomy, 2011

Research paper thumbnail of COVID-19 vaccine acceptability among health sciences students in Northwest Nigeria

Anonymized survey dataset of COVID-19 vaccine acceptability among health sciences students in Nor... more Anonymized survey dataset of COVID-19 vaccine acceptability among health sciences students in Northwest Nigeria

Research paper thumbnail of Farmers Perception for Effective Extension Teaching Methods in Katcha and Bida LGAs of Niger State, Nigeria

The study assessed farmers’ perception for effective extension teaching methods in Niger State. T... more The study assessed farmers’ perception for effective extension teaching methods in Niger State. The specific objectives were to describe the socioeconomic characteristics of farmers in the study area, identify the extension teaching methods used by extension agents in and examine the farmers’ perception of effectiveness of the extension teaching methods. Multi-stage sampling technique was used for this study. A total of 114 respondents were randomly selected from Badeggi, and Katcha in Katcha LGA, Dangana and Bida in Bida LGA. Data was collected with the aid of structured questionnaire. Descriptive statistics such as frequency and percentages and Mean scores was used to analyze objectives. The majority of the farmers (39.5%) were between ages of 20-29. The mean age was 31 years. The findings also showed that the majority (60.5%) of the respondents were male. The majority (50%) of the farmers were married, most (42.1%) had farming experience of 1-10 with a mean of 12 years. Results ...

Research paper thumbnail of Helminth Parasites of Goats and Sheep at Slaughter House in Lafia, Nasarawa State, Nigeria

FUDMA JOURNAL OF SCIENCES

Helminth parasites have been recognized as one of the major constraints to both goat and sheep pr... more Helminth parasites have been recognized as one of the major constraints to both goat and sheep production in Nigeria and other developing countries. A study aimed to determine helminth parasites of goats and sheep at slaughter in Lafia from June to July 2019. Two hundred fecal samples were collected and analyzed from 100 goats and 100 sheep using the direct smear method. The fecal sample examined revealed an overall prevalence of 155(77.5%), with goat having 80(80.0%), sheep 75(75.0%). Thirteen helminth parasites were found with nematode the most prevalent 103(66.5%), trematode had 35(22.6%), and cestode the least with 17(10.1%) and there was a statistically significant difference among the parasites (P<0.001). According to sex, females 59(43.7%) were more infected than males 25(38.5%) and there was a statistically significant difference (P<0.001). Age of sampled animals (Goat and Sheep) shows that <1 year had 28(18.1%), younger animals within the ages of <2 years 65(41....

Research paper thumbnail of Participatory Evaluation of Drought Tolerant Maize Varieties in the Guinea Savanna of Ghana Using Mother and Baby Trial Design

Maize (Zea mays) is a major food crop in Ghana but grain yields are low as a result of drought an... more Maize (Zea mays) is a major food crop in Ghana but grain yields are low as a result of drought and low soil fertility. This study evaluated drought tolerant maize varieties in 2008 and 2009 in the Guinea savanna of Ghana using researcher-managed mother and farmer-managed baby trial design. Mean grain yields ranged between 2574 and 3462 kg/ha for the mother trials and 1460 and 2328 kg/ha for baby trials. Several improved varieties performed better than the best local varieties, but two preferred varieties, TZEE Y POP STR QPM C0 and EVDT W 99 STR QPM CO which produced 35-52% more grain than the best local varieties of similar maturity rating were released in 2010. Farmers have multiple criteria for evaluating maize varieties apart from yield, though yield, larger cob and grain size were the three key criteria used by farmers to select and rank varieties. Researchers should incorporate farmers' preferences in selecting varieties in the breeding process in order to increase likelihood of adoption of the varieties. the productivity of maize-based cropping systems could increase and stabilize rural incomes, alleviate poverty and reduce food insecurity in this region (Kamara et al., 2006). Through collaborative efforts between the International Institute of Tropical Agriculture (IITA) and national maize improvement programmes in WCA, several maize varieties that are either drought tolerant (DT) or mature earlier to escape drought have been developed and evaluated under researcher-managed conditions

Research paper thumbnail of Tetrahydroxy Flavone from Acacia auriculiformis A. Cunn Ex Benth. (Fabaceae) with Novel Kinase Activity

Pharmacognosy Journal

Background: The decoctions of the bark of Acacia auriculiformis are used in folkloric medicine to... more Background: The decoctions of the bark of Acacia auriculiformis are used in folkloric medicine to relieve pain and inflammation and as remedy for cancer. Objective: The aim of this work is to screen the extract and fractions of Acacia auriculiformis for protein kinase inhibitory activity and also to isolate and characterize chemical entities from this plant and evaluate their protein kinase inhibitory activity. Materials and Methods: Kinase inhibitory activity were assayed in appropriate buffer, with either protein or peptide as substrate in the presence of 15µM (33-P) ATP (3,000Ci/mmol; 10mCi/ml) in a final volume of 30µL. Controls were performed with appropriate dilutions of dimethyl sulphoxide. A portion of the Chloroform extract, ethylacetate and n-butanol soluble fractions of the stem bark of Acacia auriculiformis were screened against a panel of disease-related protein kinases and the active fractions was tested over a wide range of concentrations from 0.016 to 50µg/ml and the IC 50 values were determined from the dose response curve. The most active fraction was subjected to chromatographic separation using Silica gel G column chromatography and sephadex LH-20 to give compound I. The structure of the isolated compound was elucidated using NMR and LC-MS. Results: The Primary screening of the extract and fractions showed that the chloroform extract was inactive against all the protein kinases investigated, while the ethylacetate and n-butanol soluble fractions inhibited all the protein kinases tested. Compound I also inhibited all the kinases tested. The IC 50 of the active fractions and compound were also evaluated. Ethylacetate fraction inhibited all the kinases tested with the highest activity against Haspine kinase with IC 50 of 1.0 µg/ml, while n-butanol also gave the highest activity against Haspine kinase with 1C 50 of 1.3 µg/ml. From the active ethylacetate fractions 3, 4', 7, 8-tetrahydroxy flavone was isolated. The Compound exhibited the maximal activity against DYRK1A kinase with an IC 50 of 2.05 µg/ml followed by CDK9 with an IC 50 of 2.28 µg/ml. Conclusion: 3, 4',7, 8-tetrahydroxy flavone was isolated was found to be a DYRK1A and CDK9 inhibitor which might justify the anticancer potential of this plant.