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Papers by Anders Brunsvik

Zenodo (CERN European Organization for Nuclear Research), Feb 24, 2023

PLOS ONE

The blockages of pipelines caused by agglomeration of gas hydrates is a major flow assurance issu... more The blockages of pipelines caused by agglomeration of gas hydrates is a major flow assurance issue in the oil and gas industry. Some crude oils form gas hydrates that remain as transportable particles in a slurry. It is commonly believed that naturally occurring components in those crude oils alter the surface properties of gas hydrate particles when formed. The exact structure of the crude oil components responsible for this surface modification remains unknown. In this study, a successive accumulation and spiking of hydrate-active crude oil fractions was performed to increase the concentration of hydrate related compounds. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) was then utilised to analyse extracted oil samples for each spiking generation. Machine learning-based variable selection was used on the FT-ICR MS spectra to identify the components related to hydrate formation. Among six different methods, Partial Least Squares Discriminant Analysis (PLS-D...

• Average mass list and logarithm scaling is preferred for PCA to separate oils in scoring plot. ... more • Average mass list and logarithm scaling is preferred for PCA to separate oils in scoring plot. Fig. 1: a) Zoom-in of LDI spectra of three different crude oil samples. b) Zoom-in of LDI spectra of three repetitive measurements showing the good reproducibility of the measurement. Fig. 2: Class distribution plot of different crude oils detected as radical cations and protonated species [H] by LDI-FTICR mass spectrometry; oil 8 and oil 8a are identical oils from different bottles; oil 9a is oil 9 one day in ion source at 3 mbar. observed with logarithm scaling than with normal scaling. However, the statistical analysis also indicated that extremely high resolution of the mass spectra is needed for the separation of the oils by PCA (Figure 4). Using bucketing with mass resolution of 2 mDa for PCA calculation instead of average mass lists resulted already in efficient separation of all crude oils. Separation of the crude oils was impossible with a mass resolution of 20 mDa bucketing for PCA simulating mass spectrum with resolving power of only 20.000. Standard deviation was low for repetitive LDI-FTICR mass measurements.

Energies

This work evaluated the effects of condensable syngas impurities on the cell viability and produc... more This work evaluated the effects of condensable syngas impurities on the cell viability and product distribution of Butyribacterium methylotrophicum in syngas fermentation. The condensates were collected during the gasification of two technical lignins derived from wheat straw (WST) and softwood (SW) at different temperatures and in the presence or absence of catalysts. The cleanest syngas with 169 and 3020 ppmv of H2S and NH3, respectively, was obtained at 800 °C using dolomite as catalyst. Pyridines were the prevalent compounds in most condensates and the highest variety of aromatics with cyanide substituents were originated during WST lignin gasification at 800 °C without catalyst. In contrast with SW lignin-based condensates, the fermentation media supplemented with WST lignin-derived condensates at 1:100 vol. only supported residual growth of B. methylotrophicum. By decreasing the condensate concentration in the medium, growth inhibition ceased and a trend toward butyrate produc...

European Journal of Pharmaceutical Sciences, 1997

Reduction of ferricytochrome C and oxidation of haemoglobin was used to examine redox properties ... more Reduction of ferricytochrome C and oxidation of haemoglobin was used to examine redox properties of primaquine, metabolites and photodegradation products of the drug. The influence of oxygen radicals (O2·− and OH·) were studied by the addition of oxygen radical scavengers. Photodecomposition of primaquine (80 mW/cm2, xenon lamp, 290–800 nm) prior to dark-incubation resulted in a substantial accelerated drug-induced O2·− formation and haemoglobin oxidation. Formation of OH· (dark reaction) could be detected after photochemical degradation of primaquine. In the presence of erythrocytes the formation of oxygen radicals induced by the photodecomposition products was even more pronounced. A high oxygen content in the medium during irradiation accelerated the photodecomposition-rate of primaquine. The metabolite 6-desmethyl primaquine was a more potent O2·− producer and haemoglobin oxidizer than primaquine (dark reactions). During irradiation (80 mW/cm2, 290–800 nm) primaquine formed more O2⋅− and produced a detectable level of OH· compared to the dark reactions.

Social Science Research Network, 2021

A novel concept for online monitoring of nitrosamines, solvent amines and their degradation produ... more A novel concept for online monitoring of nitrosamines, solvent amines and their degradation products in amine absorber emissions to air was demonstrated at the Tiller CO2-lab pilot facility. The monitoring concept is based on SINTEF patent No. PCT/EP2011/073557. The measurement method applies gas sampling by capture of analytes in a condensate stream from a single stage condensate collector unit. An improved design for the SINTEF prototype for online gas and liquid measurements has been established as part of the CCUS-ALIGN project. The new design has low complexity and improved robustness, making it applicable as integrated part of industrial monitoring systems. The established prototype was successfully tested for monitoring of trace gas emission from a CO2 capture pilot plant, demonstrating measurements of nitrosamines at levels far below the lower detection limits of commercial industrial online analyzers. Results from testing of a condensate collector prototype indicate high ca...

Applied Sciences, 2021

Most liquid food flavours such as essential oils are volatile and prone to degradation in the pre... more Most liquid food flavours such as essential oils are volatile and prone to degradation in the presence of oxygen, light, moisture and high temperatures. Microencapsulation of volatile ingredients prior to use in food or beverages is a commonly used process to limit loss and degradation of flavours and aromas during processing and storage. Here, peppermint essential oil was microencapsulated via complex coacervation using a combination of bovine serum albumin and gum Acacia as wall materials. The resulting core-shell microcapsules were chemically crosslinked with a modified food-grade starch, and subsequently spray dried, resulting in dry microcapsules which could be easily redispersed in aqueous solutions. Microcapsule formation and stability, as well as microencapsulation yield of peppermint oil, were investigated as a function of polymer concentration, core material load/wall thickness and crosslinker concentration. The crosslinked peppermint oil microcapsules were spherical and m...

Procedure Pneumocandin B0 detection and / or Pneumocandin C0 in a sample, wherein (n) is detected... more Procedure Pneumocandin B0 detection and / or Pneumocandin C0 in a sample, wherein (n) is detected a specific fragment Pneumocandin B0 of and / or a specific fragment of Pneumocandin C0 using tandem mass spectrometry in negative mode.

Journal of Porphyrins and Phthalocyanines, 2019

Aminolevulinic acid and hexyl-aminolevulinate serve as biological precursors to produce photosens... more Aminolevulinic acid and hexyl-aminolevulinate serve as biological precursors to produce photosensitive porphyrins in cells via the heme biosynthetic pathway. This pathway is integral to porphyrin-based photodynamic diagnosis and therapy. By adding exogenous hexyl-aminolevulinate to rat bladder cancer cells (AY27, in vitro) and an animal bladder cancer model (in vivo), fluorescent endogenous porphyrin production was stimulated. Lipophilic protoporphyrin IX was identified as the dominant species by reverse high-pressure liquid chromatography. Subcellular porphyrin localization in the AY27 cells was evaluated by confocal laser scanning microscopy and showed almost quantitative bleaching after 20 s. From this study, we ascertained that the protocol described herein is suitable for hexyl-aminolevulinate-mediated photodynamic therapy and diagnosis when protoporphyrin IX is the active agent.

Chemosphere, 2018

While biodegradation of chemically dispersed oil has been well documented, only a few studies hav... more While biodegradation of chemically dispersed oil has been well documented, only a few studies have focused on the degradation of the dispersant compounds themselves. The objective of this study was to determine the biodegradation of dispersant surfactants in cold seawater, relevant for deep sea or Arctic conditions. Biotransformation of the surfactants dioctyl-sodium sulfosuccinate (DOSS), Tween 80, Tween 85, and α/β-ethylhexylsulfosuccinate (EHSS, expected DOSS hydrolysis product) in the commercial dispersants Corexit 9500, Dasic Slickgone NS and Finasol OSR52 were determined. The biotransformation studies of the surfactants were performed in natural seawater at 5 °C over a period of 54 days without oil present. The surfactants were tested at concentrations of 1, 5, and 50 mg/L, the lower concentration being as close as possible to expected field concentrations. Experiments with dispersants concentrations of 1 mg/L resulted in rapid biotransformation of Tween 80 and Tween 85, with ...

Scientific reports, Jan 28, 2017

There is growing evidence from experimental and human epidemiological studies that many pollutant... more There is growing evidence from experimental and human epidemiological studies that many pollutants can disrupt lipid metabolism. In Arctic wildlife, the occurrence of such compounds could have serious consequences for seasonal feeders. We set out to study whether organohalogenated compounds (OHCs) could cause disruption of energy metabolism in female polar bears (Ursus maritimus) from Svalbard, Norway (n = 112). We analyzed biomarkers of energy metabolism including the abundance profiles of nine lipid-related genes, fatty acid (FA) synthesis and elongation indices in adipose tissue, and concentrations of lipid-related variables in plasma (cholesterol, high-density lipoprotein, triglycerides). Furthermore, the plasma metabolome and lipidome were characterized by low molecular weight metabolites and lipid fingerprinting, respectively. Polychlorinated biphenyls, chlordanes, brominated diphenyl ethers and perfluoroalkyl substances were significantly related to biomarkers involved in lip...

Energy Procedia, 2017

In the SOLVit project, degradation studies combined with chemical analysis have been an important... more In the SOLVit project, degradation studies combined with chemical analysis have been an important part of the work. New stateof-art methodology and competence have been developed and been utilized for formula identification of unknown compounds (degradation products and impurities) in solvents. This have been achieved through understanding of reaction mechanism combined with advance chemical analysis such as FT-ICR-MS (Fourier transform ion cyclotron resonance mass spectrometry). New possibilities to study the complexity of amine chemistry related to degradation and reclaiming have resulted from the program. Within the SOLVit programme, it has been possible to optimize and qualify different solvent systems based on their degradation properties and chemistry. The SOLVit programme has created important generic information and competence on degradation chemistry and chemical analysis that can be of great value in the future.

The present invention concerns a method of detecting the antifungal cyclic hexapeptides Pneumocan... more The present invention concerns a method of detecting the antifungal cyclic hexapeptides Pneumocandin B.sub.0 and/or Pneumocandin C.sub.0 specific fragment is/are detected using MS in negative mode.

Journal of the Serbian Chemical Society, 2016

The activation of PLA2 by means of pro-inflammatory cytokines results in the subsequent release o... more The activation of PLA2 by means of pro-inflammatory cytokines results in the subsequent release of arachidonic acid (AA) and generates eicosanoids, which further propagate inflammation. By 6the cyclooxygenases (COX1/2) responsible for the enzymatic conversion of AA to eicosanoids, the non-steroidal anti-inflammatory drugs are effective in relieving the pain and discomfort of inflammation. By using AA release assay as a guide for biological and anti-inflammatory activity, novel compound 1-O-(3-O-linolenoyl-6-deoxy-6-sulfo-?-D-glucopyranosyl)-glycerol (1) together with five known compounds isovitexin, byzantionoside B, tricin 4?-O-(erythro-?-guaiacylglyceryl) ether 7-O-?-glucopyranoside, 1-O-feruloyl glycerol and tricin 7-glucoside were isolated from the methanol extract of the aerial parts of Sclerochloa dura using LC techniques (Sephadex LH-20 column chromatography, preparative HPLC and semi-preparative HPLC). All isolated compounds were identified using spectroscopic NMR spectrosco...

Zenodo (CERN European Organization for Nuclear Research), Feb 24, 2023

PLOS ONE

The blockages of pipelines caused by agglomeration of gas hydrates is a major flow assurance issu... more The blockages of pipelines caused by agglomeration of gas hydrates is a major flow assurance issue in the oil and gas industry. Some crude oils form gas hydrates that remain as transportable particles in a slurry. It is commonly believed that naturally occurring components in those crude oils alter the surface properties of gas hydrate particles when formed. The exact structure of the crude oil components responsible for this surface modification remains unknown. In this study, a successive accumulation and spiking of hydrate-active crude oil fractions was performed to increase the concentration of hydrate related compounds. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) was then utilised to analyse extracted oil samples for each spiking generation. Machine learning-based variable selection was used on the FT-ICR MS spectra to identify the components related to hydrate formation. Among six different methods, Partial Least Squares Discriminant Analysis (PLS-D...

• Average mass list and logarithm scaling is preferred for PCA to separate oils in scoring plot. ... more • Average mass list and logarithm scaling is preferred for PCA to separate oils in scoring plot. Fig. 1: a) Zoom-in of LDI spectra of three different crude oil samples. b) Zoom-in of LDI spectra of three repetitive measurements showing the good reproducibility of the measurement. Fig. 2: Class distribution plot of different crude oils detected as radical cations and protonated species [H] by LDI-FTICR mass spectrometry; oil 8 and oil 8a are identical oils from different bottles; oil 9a is oil 9 one day in ion source at 3 mbar. observed with logarithm scaling than with normal scaling. However, the statistical analysis also indicated that extremely high resolution of the mass spectra is needed for the separation of the oils by PCA (Figure 4). Using bucketing with mass resolution of 2 mDa for PCA calculation instead of average mass lists resulted already in efficient separation of all crude oils. Separation of the crude oils was impossible with a mass resolution of 20 mDa bucketing for PCA simulating mass spectrum with resolving power of only 20.000. Standard deviation was low for repetitive LDI-FTICR mass measurements.

Energies

This work evaluated the effects of condensable syngas impurities on the cell viability and produc... more This work evaluated the effects of condensable syngas impurities on the cell viability and product distribution of Butyribacterium methylotrophicum in syngas fermentation. The condensates were collected during the gasification of two technical lignins derived from wheat straw (WST) and softwood (SW) at different temperatures and in the presence or absence of catalysts. The cleanest syngas with 169 and 3020 ppmv of H2S and NH3, respectively, was obtained at 800 °C using dolomite as catalyst. Pyridines were the prevalent compounds in most condensates and the highest variety of aromatics with cyanide substituents were originated during WST lignin gasification at 800 °C without catalyst. In contrast with SW lignin-based condensates, the fermentation media supplemented with WST lignin-derived condensates at 1:100 vol. only supported residual growth of B. methylotrophicum. By decreasing the condensate concentration in the medium, growth inhibition ceased and a trend toward butyrate produc...

European Journal of Pharmaceutical Sciences, 1997

Reduction of ferricytochrome C and oxidation of haemoglobin was used to examine redox properties ... more Reduction of ferricytochrome C and oxidation of haemoglobin was used to examine redox properties of primaquine, metabolites and photodegradation products of the drug. The influence of oxygen radicals (O2·− and OH·) were studied by the addition of oxygen radical scavengers. Photodecomposition of primaquine (80 mW/cm2, xenon lamp, 290–800 nm) prior to dark-incubation resulted in a substantial accelerated drug-induced O2·− formation and haemoglobin oxidation. Formation of OH· (dark reaction) could be detected after photochemical degradation of primaquine. In the presence of erythrocytes the formation of oxygen radicals induced by the photodecomposition products was even more pronounced. A high oxygen content in the medium during irradiation accelerated the photodecomposition-rate of primaquine. The metabolite 6-desmethyl primaquine was a more potent O2·− producer and haemoglobin oxidizer than primaquine (dark reactions). During irradiation (80 mW/cm2, 290–800 nm) primaquine formed more O2⋅− and produced a detectable level of OH· compared to the dark reactions.

Social Science Research Network, 2021

A novel concept for online monitoring of nitrosamines, solvent amines and their degradation produ... more A novel concept for online monitoring of nitrosamines, solvent amines and their degradation products in amine absorber emissions to air was demonstrated at the Tiller CO2-lab pilot facility. The monitoring concept is based on SINTEF patent No. PCT/EP2011/073557. The measurement method applies gas sampling by capture of analytes in a condensate stream from a single stage condensate collector unit. An improved design for the SINTEF prototype for online gas and liquid measurements has been established as part of the CCUS-ALIGN project. The new design has low complexity and improved robustness, making it applicable as integrated part of industrial monitoring systems. The established prototype was successfully tested for monitoring of trace gas emission from a CO2 capture pilot plant, demonstrating measurements of nitrosamines at levels far below the lower detection limits of commercial industrial online analyzers. Results from testing of a condensate collector prototype indicate high ca...

Applied Sciences, 2021

Most liquid food flavours such as essential oils are volatile and prone to degradation in the pre... more Most liquid food flavours such as essential oils are volatile and prone to degradation in the presence of oxygen, light, moisture and high temperatures. Microencapsulation of volatile ingredients prior to use in food or beverages is a commonly used process to limit loss and degradation of flavours and aromas during processing and storage. Here, peppermint essential oil was microencapsulated via complex coacervation using a combination of bovine serum albumin and gum Acacia as wall materials. The resulting core-shell microcapsules were chemically crosslinked with a modified food-grade starch, and subsequently spray dried, resulting in dry microcapsules which could be easily redispersed in aqueous solutions. Microcapsule formation and stability, as well as microencapsulation yield of peppermint oil, were investigated as a function of polymer concentration, core material load/wall thickness and crosslinker concentration. The crosslinked peppermint oil microcapsules were spherical and m...

Procedure Pneumocandin B0 detection and / or Pneumocandin C0 in a sample, wherein (n) is detected... more Procedure Pneumocandin B0 detection and / or Pneumocandin C0 in a sample, wherein (n) is detected a specific fragment Pneumocandin B0 of and / or a specific fragment of Pneumocandin C0 using tandem mass spectrometry in negative mode.

Journal of Porphyrins and Phthalocyanines, 2019

Aminolevulinic acid and hexyl-aminolevulinate serve as biological precursors to produce photosens... more Aminolevulinic acid and hexyl-aminolevulinate serve as biological precursors to produce photosensitive porphyrins in cells via the heme biosynthetic pathway. This pathway is integral to porphyrin-based photodynamic diagnosis and therapy. By adding exogenous hexyl-aminolevulinate to rat bladder cancer cells (AY27, in vitro) and an animal bladder cancer model (in vivo), fluorescent endogenous porphyrin production was stimulated. Lipophilic protoporphyrin IX was identified as the dominant species by reverse high-pressure liquid chromatography. Subcellular porphyrin localization in the AY27 cells was evaluated by confocal laser scanning microscopy and showed almost quantitative bleaching after 20 s. From this study, we ascertained that the protocol described herein is suitable for hexyl-aminolevulinate-mediated photodynamic therapy and diagnosis when protoporphyrin IX is the active agent.

Chemosphere, 2018

While biodegradation of chemically dispersed oil has been well documented, only a few studies hav... more While biodegradation of chemically dispersed oil has been well documented, only a few studies have focused on the degradation of the dispersant compounds themselves. The objective of this study was to determine the biodegradation of dispersant surfactants in cold seawater, relevant for deep sea or Arctic conditions. Biotransformation of the surfactants dioctyl-sodium sulfosuccinate (DOSS), Tween 80, Tween 85, and α/β-ethylhexylsulfosuccinate (EHSS, expected DOSS hydrolysis product) in the commercial dispersants Corexit 9500, Dasic Slickgone NS and Finasol OSR52 were determined. The biotransformation studies of the surfactants were performed in natural seawater at 5 °C over a period of 54 days without oil present. The surfactants were tested at concentrations of 1, 5, and 50 mg/L, the lower concentration being as close as possible to expected field concentrations. Experiments with dispersants concentrations of 1 mg/L resulted in rapid biotransformation of Tween 80 and Tween 85, with ...

Scientific reports, Jan 28, 2017

There is growing evidence from experimental and human epidemiological studies that many pollutant... more There is growing evidence from experimental and human epidemiological studies that many pollutants can disrupt lipid metabolism. In Arctic wildlife, the occurrence of such compounds could have serious consequences for seasonal feeders. We set out to study whether organohalogenated compounds (OHCs) could cause disruption of energy metabolism in female polar bears (Ursus maritimus) from Svalbard, Norway (n = 112). We analyzed biomarkers of energy metabolism including the abundance profiles of nine lipid-related genes, fatty acid (FA) synthesis and elongation indices in adipose tissue, and concentrations of lipid-related variables in plasma (cholesterol, high-density lipoprotein, triglycerides). Furthermore, the plasma metabolome and lipidome were characterized by low molecular weight metabolites and lipid fingerprinting, respectively. Polychlorinated biphenyls, chlordanes, brominated diphenyl ethers and perfluoroalkyl substances were significantly related to biomarkers involved in lip...

Energy Procedia, 2017

In the SOLVit project, degradation studies combined with chemical analysis have been an important... more In the SOLVit project, degradation studies combined with chemical analysis have been an important part of the work. New stateof-art methodology and competence have been developed and been utilized for formula identification of unknown compounds (degradation products and impurities) in solvents. This have been achieved through understanding of reaction mechanism combined with advance chemical analysis such as FT-ICR-MS (Fourier transform ion cyclotron resonance mass spectrometry). New possibilities to study the complexity of amine chemistry related to degradation and reclaiming have resulted from the program. Within the SOLVit programme, it has been possible to optimize and qualify different solvent systems based on their degradation properties and chemistry. The SOLVit programme has created important generic information and competence on degradation chemistry and chemical analysis that can be of great value in the future.

The present invention concerns a method of detecting the antifungal cyclic hexapeptides Pneumocan... more The present invention concerns a method of detecting the antifungal cyclic hexapeptides Pneumocandin B.sub.0 and/or Pneumocandin C.sub.0 specific fragment is/are detected using MS in negative mode.

Journal of the Serbian Chemical Society, 2016

The activation of PLA2 by means of pro-inflammatory cytokines results in the subsequent release o... more The activation of PLA2 by means of pro-inflammatory cytokines results in the subsequent release of arachidonic acid (AA) and generates eicosanoids, which further propagate inflammation. By 6the cyclooxygenases (COX1/2) responsible for the enzymatic conversion of AA to eicosanoids, the non-steroidal anti-inflammatory drugs are effective in relieving the pain and discomfort of inflammation. By using AA release assay as a guide for biological and anti-inflammatory activity, novel compound 1-O-(3-O-linolenoyl-6-deoxy-6-sulfo-?-D-glucopyranosyl)-glycerol (1) together with five known compounds isovitexin, byzantionoside B, tricin 4?-O-(erythro-?-guaiacylglyceryl) ether 7-O-?-glucopyranoside, 1-O-feruloyl glycerol and tricin 7-glucoside were isolated from the methanol extract of the aerial parts of Sclerochloa dura using LC techniques (Sephadex LH-20 column chromatography, preparative HPLC and semi-preparative HPLC). All isolated compounds were identified using spectroscopic NMR spectrosco...