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Papers by Anders Malmstrom

Biochemical Journal, Feb 1, 1983

Frontiers in immunology, 2018

For full activation of naïve adaptive lymphocytes in skin-draining lymph nodes (LNs), presentatio... more For full activation of naïve adaptive lymphocytes in skin-draining lymph nodes (LNs), presentation of peptide:MHC complexes by LN-resident and skin-derived dendritic cells (DCs) that encountered antigens (Ags) is an absolute prerequisite. To get to the nearest draining LN upon intradermal immunization, DCs need to migrate from the infection site to the afferent lymphatics, which can only be reached by traversing a collagen-dense network located in the dermis of the skin through the activity of proteolytic enzymes. Here, we show that mice with altered collagen fibrillogenesis resulting in thicker collagen fibers in the skin display a reduced DC migration to the draining LN upon immune challenge. Consequently, the initiation of the cellular and humoral immune response was diminished. Ag-specific CD8+ and CD4+ T cells as well as Ag-specific germinal center B cells and serum immunoglobulin levels were significantly decreased. Hence, we postulate that alterations to the production of ext...

Journal of proteomics, 2018

Remodeling of the extracellular matrix (ECM) is a common feature in lung diseases such as chronic... more Remodeling of the extracellular matrix (ECM) is a common feature in lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF). Here, we applied a sequential tissue extraction strategy to describe disease-specific remodeling of human lung tissue in disease, using end-stages of COPD and IPF. Our strategy was based on quantitative comparison of the disease proteomes, with specific focus on the matrisome, using data-independent acquisition and targeted data analysis (SWATH-MS). Our work provides an in-depth proteomic characterization of human lung tissue during impaired tissue remodeling. In addition, we show important quantitative and qualitative effects of the solubility of matrisome proteins. COPD was characterized by a disease-specific increase in ECM regulators, metalloproteinase inhibitor 3 (TIMP3) and matrix metalloproteinase 28 (MMP-28), whereas for IPF, impairment in cell adhesion proteins, such as collagen VI and laminins, was m...

Xiiith International Symposium on Glyco Conjugates 20 26 Augusti Seattle Usa Abstract, 1995

Defructosylated capsular polysaccharide from Escherichia coli strain K4 can be converted into der... more Defructosylated capsular polysaccharide from Escherichia coli strain K4 can be converted into dermatan by a glucuronyl-C5-epimerase.

Histochemistry Cell Biol, 2009

Journal of Biological Chemistry, 2016

Disease models & mechanisms, Jan 21, 2016

Of all live births with congenital anomalies, approximately one-third exhibit deformities of the ... more Of all live births with congenital anomalies, approximately one-third exhibit deformities of the head and face. Most craniofacial disorders are associated with defects in a migratory stem and progenitor cell population, which is designated the neural crest (NC). Musculocontractural Ehlers-Danlos syndrome (MCEDS) is a heritable connective tissue disorder with distinct craniofacial features; this syndrome comprises multiple congenital malformations that are caused by dysfunction of dermatan sulfate (DS) biosynthetic enzymes, including DS epimerase-1 (DS-epi1). Studies in mice have extended our understanding of DS-epi1 in connective tissue maintenance; however, its role in fetal development is not understood. We demonstrate that DS-epi1 is important for the generation of isolated iduronic acid residues in chondroitin sulfate (CS)/DS proteoglycans in early ITALIC! Xenopusembryos. The knockdown of DS-epi1 does not affect the formation of early NC progenitors; however, it impairs the corr...

The Journal of Immunology, Apr 1, 2011

Chem. Sci., 2016

DS-epi1 is a processive enzyme that sequentially epimerizes polysaccharide substrate towards the ... more DS-epi1 is a processive enzyme that sequentially epimerizes polysaccharide substrate towards the non-reducing end.

Journal of Biological Chemistry

Biochemical Journal, Feb 1, 1983

Frontiers in immunology, 2018

For full activation of naïve adaptive lymphocytes in skin-draining lymph nodes (LNs), presentatio... more For full activation of naïve adaptive lymphocytes in skin-draining lymph nodes (LNs), presentation of peptide:MHC complexes by LN-resident and skin-derived dendritic cells (DCs) that encountered antigens (Ags) is an absolute prerequisite. To get to the nearest draining LN upon intradermal immunization, DCs need to migrate from the infection site to the afferent lymphatics, which can only be reached by traversing a collagen-dense network located in the dermis of the skin through the activity of proteolytic enzymes. Here, we show that mice with altered collagen fibrillogenesis resulting in thicker collagen fibers in the skin display a reduced DC migration to the draining LN upon immune challenge. Consequently, the initiation of the cellular and humoral immune response was diminished. Ag-specific CD8+ and CD4+ T cells as well as Ag-specific germinal center B cells and serum immunoglobulin levels were significantly decreased. Hence, we postulate that alterations to the production of ext...

Journal of proteomics, 2018

Remodeling of the extracellular matrix (ECM) is a common feature in lung diseases such as chronic... more Remodeling of the extracellular matrix (ECM) is a common feature in lung diseases such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF). Here, we applied a sequential tissue extraction strategy to describe disease-specific remodeling of human lung tissue in disease, using end-stages of COPD and IPF. Our strategy was based on quantitative comparison of the disease proteomes, with specific focus on the matrisome, using data-independent acquisition and targeted data analysis (SWATH-MS). Our work provides an in-depth proteomic characterization of human lung tissue during impaired tissue remodeling. In addition, we show important quantitative and qualitative effects of the solubility of matrisome proteins. COPD was characterized by a disease-specific increase in ECM regulators, metalloproteinase inhibitor 3 (TIMP3) and matrix metalloproteinase 28 (MMP-28), whereas for IPF, impairment in cell adhesion proteins, such as collagen VI and laminins, was m...

Xiiith International Symposium on Glyco Conjugates 20 26 Augusti Seattle Usa Abstract, 1995

Defructosylated capsular polysaccharide from Escherichia coli strain K4 can be converted into der... more Defructosylated capsular polysaccharide from Escherichia coli strain K4 can be converted into dermatan by a glucuronyl-C5-epimerase.

Histochemistry Cell Biol, 2009

Journal of Biological Chemistry, 2016

Disease models & mechanisms, Jan 21, 2016

Of all live births with congenital anomalies, approximately one-third exhibit deformities of the ... more Of all live births with congenital anomalies, approximately one-third exhibit deformities of the head and face. Most craniofacial disorders are associated with defects in a migratory stem and progenitor cell population, which is designated the neural crest (NC). Musculocontractural Ehlers-Danlos syndrome (MCEDS) is a heritable connective tissue disorder with distinct craniofacial features; this syndrome comprises multiple congenital malformations that are caused by dysfunction of dermatan sulfate (DS) biosynthetic enzymes, including DS epimerase-1 (DS-epi1). Studies in mice have extended our understanding of DS-epi1 in connective tissue maintenance; however, its role in fetal development is not understood. We demonstrate that DS-epi1 is important for the generation of isolated iduronic acid residues in chondroitin sulfate (CS)/DS proteoglycans in early ITALIC! Xenopusembryos. The knockdown of DS-epi1 does not affect the formation of early NC progenitors; however, it impairs the corr...

The Journal of Immunology, Apr 1, 2011

Chem. Sci., 2016

DS-epi1 is a processive enzyme that sequentially epimerizes polysaccharide substrate towards the ... more DS-epi1 is a processive enzyme that sequentially epimerizes polysaccharide substrate towards the non-reducing end.

Journal of Biological Chemistry

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