Andini Sundowo - Academia.edu (original) (raw)
Papers by Andini Sundowo
A crude peroxidase biocatalyst extracted from sawi hijau ( Brassica juncea ) was used to produce ... more A crude peroxidase biocatalyst extracted from sawi hijau ( Brassica juncea ) was used to produce dimerization phenolic synthesized from eugenol. The result from MS and NMR analysis showed that the reaction between eugenol substrate and H 2 O 2 with crude extract enzyme produced a new chemical compound. The chemical structure of new compound showed coupled result in ortho-ortho position with C-C coupled derivative, dehydrodieugenol. It was obtained as brownish white crystal needles with yield 10%. This compound displayed higher cytotoxicity effect on T47D breast cancer cell compared to the eugenol monomer.
Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and s... more Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and sodium sulfite has been studied. The process involves two steps of reactions, the first was esterification of ethanolamine (H 2 N-CH 2-CH 2-OH) with sulfuric acid to produce the intermediate product of 2-aminoethyl ester (H 2 N-CH 2-CH 2-OSO 3 H) which then was extended to the second step by sulfonation with sodium sulfite to produce 2-aminoethylsulfonic acid (H 2 N-CH 2-CH 2-SO 3 H). These two process conditions were observed by varying mole ratio of reactants, temperature and time period of reactions. Taurine product was qualitatively analyzed using 1 H-NMR and LC-MS. Physical-chemical analysis were done by observing its melting point and determining its water, chloride and sulfate contents. Its melting point, water content, and sulfate content were 290 o C, 0.303%, and 3 ppm, respectively, while its chloride content was undetected. After purification, the yield of process was 25.57%.
The Natural Products Journal
Background: Marine sponges provided a great source of natural products with promising biological ... more Background: Marine sponges provided a great source of natural products with promising biological activity. This study was aimed to investigate the chemical constituents of methanol extracts of selected Indonesian marine sponges (Callyspongia sp., Clathria sp., Melophlus sarasinorum, and Xestospongia sp.), collected from the Saponda Islands, Southeast Sulawesi, Indonesia as well as to evaluate their antimicrobial and antioxidant activities. Methods: LCMS/MS analysis used to identify the compounds. Agar well diffusion and DPPH assays were used to evaluate the antimicrobial and antioxidant activities. Results: Chemical screening reported alkaloids, terpenoids, steroids, and saponins from all investigated sponges. The LC-MS/MS analysis identified various compounds which mainly contained steroids. Antimicrobial activity (against Bacillus subtilis, Escherichia coli, Salmonella enterica, and Candida albicans) was only shown by the Xestospongia sp. extract. Meanwhile, extracts of M. sarasin...
Current Agriculture Research Journal
Rubus fraxinifolius is a genus that belongs to the Rosaceae family, which grows in the mountainou... more Rubus fraxinifolius is a genus that belongs to the Rosaceae family, which grows in the mountainous forests in Indonesia. The study aimed to determine the effects of air-drying (AD) and oven drying (OD) of the young R. fraxinifolius leaves and compare them to the fresh leaves (FL) to observe the phytochemical content and bioactivities. Phytochemical contents of the samples were determined based on qualitative assay, total phenolics, and total flavonoid, whereas bioactivities were evaluated on antioxidant DPPH free radical scavenging effect, α-glucosidase inhibitory activity, and antibacterial assays. LCMS-MS was used for the quantitative investigation of naturally occurring phytochemicals. The methanol exttract of FL had the highest free radical scavenging activity against DPPH with IC50of 45.51± 5.03µg/mL, total phenolic content (25.35 ± 1.06 mg GAE/100 mg extract), and total flavonoid content (28.71 ± 2.07 equivalent quercetin mg/ 100 mg extract) compared to those of AD and OD. How...
PROCEEDINGS OF THE 5TH INTERNATIONAL SYMPOSIUM ON APPLIED CHEMISTRY 2019
PROCEEDINGS OF THE 5TH INTERNATIONAL SYMPOSIUM ON APPLIED CHEMISTRY 2019
Jurnal Teknologi Pangan dan Gizi
Pada pembuatan yogurt terjadi konversi gula susu (laktosa) menjadi asam laktat melalui proses fer... more Pada pembuatan yogurt terjadi konversi gula susu (laktosa) menjadi asam laktat melalui proses fermentasi oleh bakteri asam laktat (BAL). Konsentrasi stater dan waktu fermentasi yang digunakan akan mempengaruhi kecepatan perombakan laktosa menjadi asam laktat. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan stater dengan variasi sebesar : 1, 5, 10, 15 dan 20% dan waktu fermentasi pada 24 jam dan 48 jam terhadap asam laktat. Parameter yang diuji diantaranya adalah pH, kadar laktosa, kadar protein, kadar asam lakat, dan aktivitas antioksidan. Parameter yang diuji dari hasil proses fermentasi adalah pH, laktosa, asam laktat, protein terlarut. Pengujian laktosa dan asam laktat dilakukan dengan menggunakan HPLC, pengujian konsentrasi protein terlarut dengan menggunakan metode Lowry. Asam laktosa dan asam laktat tertinggi diperoleh dari fermentasi dengan variasi konsentrasi BAL starter 15% dalam waktu fermentasi selama 24 jam. Hasil analisis % inhibisi pada yogurt konsentrasi...
![Research paper thumbnail of Computer-aided Structure-based Design of 3,3'-Diallyl-[1,1'-biphenyl]- 4,4'- diol Analogs of Eugenol as Potential Ligands for Estrogen Receptor Alpha](https://attachments.academia-assets.com/69101018/thumbnails/1.jpg)
](Proceedings of the 3rd International Conference on Computation for Science and Technology, 2015
The SBVS protocols to identify ligands for estrogen receptor alpha (ERα) were retrospectively val... more The SBVS protocols to identify ligands for estrogen receptor alpha (ERα) were retrospectively validated and could serve as a virtual tool to screen potential ERα ligands. Subsequently, prospective virtual screen campaigns on eugenol analogs and their dimers were performed and have discovered 3,3'-diallyl-[1,1'-biphenyl]-4,4'-diol analogs of eugenol as the most potential ERα fragment to be further developed. This article presents the design of 3,3'-diallyl-[1,1'-biphenyl]-4,4'-diol analogs of eugenol based on the comparison between the docked poses of ZINC01914469 as a reference compound and 3,3'-diallyl-[1,1'-biphenyl]-4,4'-diol analogs of eugenol. The designed compounds were selected based on the docking score using the validated SBVS protocols. The docking scores of the selected compounds revealed that these compounds were statistically similar or better than ZINC01914469.
MAKARA of Science Series, 2010
Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and s... more Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and sodium sulfite has been studied. The process involves two steps of reactions, the first was esterification of ethanolamine (H 2 N-CH 2-CH 2-OH) with sulfuric acid to produce the intermediate product of 2-aminoethyl ester (H 2 N-CH 2-CH 2-OSO 3 H) which then was extended to the second step by sulfonation with sodium sulfite to produce 2-aminoethylsulfonic acid (H 2 N-CH 2-CH 2-SO 3 H). These two process conditions were observed by varying mole ratio of reactants, temperature and time period of reactions. Taurine product was qualitatively analyzed using 1 H-NMR and LC-MS. Physical-chemical analysis were done by observing its melting point and determining its water, chloride and sulfate contents. Its melting point, water content, and sulfate content were 290 o C, 0.303%, and 3 ppm, respectively, while its chloride content was undetected. After purification, the yield of process was 25.57%.
Indonesian Journal of Chemistry, Jun 24, 2010
The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Art... more The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Artocarpus communis leaves using column chromatography techniques. The structure of the flavanone compound was elucidated on the basic of spectroscopic evidence and comparison to published values. This compound, 8-geranyl-4,5,7-trihydroxyflavone, showed strong anti diabetic activity on α-glucosidase inhibition assay with IC 50 18.120 µg mL-1 .
Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attribut... more Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attributed it to the presence of polyphenols such as catechin. The objective of this study is to investigate the potential of various gambiers available in Indonesian market as a scavenger of reactive free radicals and evaluate its anti-hyperglycemic activity as αglucosidase inhibitor. Isolation of catechin was done by extraction method with technical grade of ethyl acetate as solvent. Analysis of catechin in the dried gambier extract was carried out with TLC method. The molecular weight and content of catechin of dried gambier extract was determined by analyzing its mass spectra and spectrophotometer, respectively. The free radical scavenging activity of catechin of the resultant extracts was measured by using 1,1diphenyl-2-picrylhydrazyl (DPPH) as stable free radical compound. The anti-hyperglycemic activity of catechin of ethyl acetate extracts was analyzed as α-glucosidase inhibitor. The result showed that various gambiers available in the market are very active as antioxidant, indicated by IC 50 of catechin of ethyl acetate extracts which were 4.6 to 18.2 μg/mL for DPPH inhibition. The IC 50 for α-glucosidase inhibition is ranged from 40.45 to 52.43 μg/mL, so they can be classified as anti-diabetic.
Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attribut... more Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attributed it to the presence of polyphenols such as catechin. The objective of this study is to investigate the potential of various gambiers available in Indonesian market as a scavenger of reactive free radicals and evaluate its anti-hyperglycemic activity as αglucosidase inhibitor. Isolation of catechin was done by extraction method with technical grade of ethyl acetate as solvent. Analysis of catechin in the dried gambier extract was carried out with TLC method. The molecular weight and content of catechin of dried gambier extract was determined by analyzing its mass spectra and spectrophotometer, respectively. The free radical scavenging activity of catechin of the resultant extracts was measured by using 1,1diphenyl-2-picrylhydrazyl (DPPH) as stable free radical compound. The anti-hyperglycemic activity of catechin of ethyl acetate extracts was analyzed as α-glucosidase inhibitor. The result showed that various gambiers available in the market are very active as antioxidant, indicated by IC 50 of catechin of ethyl acetate extracts which were 4.6 to 18.2 μg/mL for DPPH inhibition. The IC 50 for α-glucosidase inhibition is ranged from 40.45 to 52.43 μg/mL, so they can be classified as anti-diabetic.
A crude peroxidase biocatalyst extracted from sawi hijau ( Brassica juncea ) was used to produce ... more A crude peroxidase biocatalyst extracted from sawi hijau ( Brassica juncea ) was used to produce dimerization phenolic synthesized from eugenol. The result from MS and NMR analysis showed that the reaction between eugenol substrate and H 2 O 2 with crude extract enzyme produced a new chemical compound. The chemical structure of new compound showed coupled result in ortho-ortho position with C-C coupled derivative, dehydrodieugenol. It was obtained as brownish white crystal needles with yield 10%. This compound displayed higher cytotoxicity effect on T47D breast cancer cell compared to the eugenol monomer.
Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and s... more Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and sodium sulfite has been studied. The process involves two steps of reactions, the first was esterification of ethanolamine (H 2 N-CH 2-CH 2-OH) with sulfuric acid to produce the intermediate product of 2-aminoethyl ester (H 2 N-CH 2-CH 2-OSO 3 H) which then was extended to the second step by sulfonation with sodium sulfite to produce 2-aminoethylsulfonic acid (H 2 N-CH 2-CH 2-SO 3 H). These two process conditions were observed by varying mole ratio of reactants, temperature and time period of reactions. Taurine product was qualitatively analyzed using 1 H-NMR and LC-MS. Physical-chemical analysis were done by observing its melting point and determining its water, chloride and sulfate contents. Its melting point, water content, and sulfate content were 290 o C, 0.303%, and 3 ppm, respectively, while its chloride content was undetected. After purification, the yield of process was 25.57%.
The Natural Products Journal
Background: Marine sponges provided a great source of natural products with promising biological ... more Background: Marine sponges provided a great source of natural products with promising biological activity. This study was aimed to investigate the chemical constituents of methanol extracts of selected Indonesian marine sponges (Callyspongia sp., Clathria sp., Melophlus sarasinorum, and Xestospongia sp.), collected from the Saponda Islands, Southeast Sulawesi, Indonesia as well as to evaluate their antimicrobial and antioxidant activities. Methods: LCMS/MS analysis used to identify the compounds. Agar well diffusion and DPPH assays were used to evaluate the antimicrobial and antioxidant activities. Results: Chemical screening reported alkaloids, terpenoids, steroids, and saponins from all investigated sponges. The LC-MS/MS analysis identified various compounds which mainly contained steroids. Antimicrobial activity (against Bacillus subtilis, Escherichia coli, Salmonella enterica, and Candida albicans) was only shown by the Xestospongia sp. extract. Meanwhile, extracts of M. sarasin...
Current Agriculture Research Journal
Rubus fraxinifolius is a genus that belongs to the Rosaceae family, which grows in the mountainou... more Rubus fraxinifolius is a genus that belongs to the Rosaceae family, which grows in the mountainous forests in Indonesia. The study aimed to determine the effects of air-drying (AD) and oven drying (OD) of the young R. fraxinifolius leaves and compare them to the fresh leaves (FL) to observe the phytochemical content and bioactivities. Phytochemical contents of the samples were determined based on qualitative assay, total phenolics, and total flavonoid, whereas bioactivities were evaluated on antioxidant DPPH free radical scavenging effect, α-glucosidase inhibitory activity, and antibacterial assays. LCMS-MS was used for the quantitative investigation of naturally occurring phytochemicals. The methanol exttract of FL had the highest free radical scavenging activity against DPPH with IC50of 45.51± 5.03µg/mL, total phenolic content (25.35 ± 1.06 mg GAE/100 mg extract), and total flavonoid content (28.71 ± 2.07 equivalent quercetin mg/ 100 mg extract) compared to those of AD and OD. How...
PROCEEDINGS OF THE 5TH INTERNATIONAL SYMPOSIUM ON APPLIED CHEMISTRY 2019
PROCEEDINGS OF THE 5TH INTERNATIONAL SYMPOSIUM ON APPLIED CHEMISTRY 2019
Jurnal Teknologi Pangan dan Gizi
Pada pembuatan yogurt terjadi konversi gula susu (laktosa) menjadi asam laktat melalui proses fer... more Pada pembuatan yogurt terjadi konversi gula susu (laktosa) menjadi asam laktat melalui proses fermentasi oleh bakteri asam laktat (BAL). Konsentrasi stater dan waktu fermentasi yang digunakan akan mempengaruhi kecepatan perombakan laktosa menjadi asam laktat. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan stater dengan variasi sebesar : 1, 5, 10, 15 dan 20% dan waktu fermentasi pada 24 jam dan 48 jam terhadap asam laktat. Parameter yang diuji diantaranya adalah pH, kadar laktosa, kadar protein, kadar asam lakat, dan aktivitas antioksidan. Parameter yang diuji dari hasil proses fermentasi adalah pH, laktosa, asam laktat, protein terlarut. Pengujian laktosa dan asam laktat dilakukan dengan menggunakan HPLC, pengujian konsentrasi protein terlarut dengan menggunakan metode Lowry. Asam laktosa dan asam laktat tertinggi diperoleh dari fermentasi dengan variasi konsentrasi BAL starter 15% dalam waktu fermentasi selama 24 jam. Hasil analisis % inhibisi pada yogurt konsentrasi...
Proceedings of the 3rd International Conference on Computation for Science and Technology, 2015
The SBVS protocols to identify ligands for estrogen receptor alpha (ERα) were retrospectively val... more The SBVS protocols to identify ligands for estrogen receptor alpha (ERα) were retrospectively validated and could serve as a virtual tool to screen potential ERα ligands. Subsequently, prospective virtual screen campaigns on eugenol analogs and their dimers were performed and have discovered 3,3'-diallyl-[1,1'-biphenyl]-4,4'-diol analogs of eugenol as the most potential ERα fragment to be further developed. This article presents the design of 3,3'-diallyl-[1,1'-biphenyl]-4,4'-diol analogs of eugenol based on the comparison between the docked poses of ZINC01914469 as a reference compound and 3,3'-diallyl-[1,1'-biphenyl]-4,4'-diol analogs of eugenol. The designed compounds were selected based on the docking score using the validated SBVS protocols. The docking scores of the selected compounds revealed that these compounds were statistically similar or better than ZINC01914469.
MAKARA of Science Series, 2010
Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and s... more Preparation process of 2-aminoethylsulfonic acid (taurine) from ethanolamine, sulfuric acid and sodium sulfite has been studied. The process involves two steps of reactions, the first was esterification of ethanolamine (H 2 N-CH 2-CH 2-OH) with sulfuric acid to produce the intermediate product of 2-aminoethyl ester (H 2 N-CH 2-CH 2-OSO 3 H) which then was extended to the second step by sulfonation with sodium sulfite to produce 2-aminoethylsulfonic acid (H 2 N-CH 2-CH 2-SO 3 H). These two process conditions were observed by varying mole ratio of reactants, temperature and time period of reactions. Taurine product was qualitatively analyzed using 1 H-NMR and LC-MS. Physical-chemical analysis were done by observing its melting point and determining its water, chloride and sulfate contents. Its melting point, water content, and sulfate content were 290 o C, 0.303%, and 3 ppm, respectively, while its chloride content was undetected. After purification, the yield of process was 25.57%.
Indonesian Journal of Chemistry, Jun 24, 2010
The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Art... more The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Artocarpus communis leaves using column chromatography techniques. The structure of the flavanone compound was elucidated on the basic of spectroscopic evidence and comparison to published values. This compound, 8-geranyl-4,5,7-trihydroxyflavone, showed strong anti diabetic activity on α-glucosidase inhibition assay with IC 50 18.120 µg mL-1 .
Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attribut... more Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attributed it to the presence of polyphenols such as catechin. The objective of this study is to investigate the potential of various gambiers available in Indonesian market as a scavenger of reactive free radicals and evaluate its anti-hyperglycemic activity as αglucosidase inhibitor. Isolation of catechin was done by extraction method with technical grade of ethyl acetate as solvent. Analysis of catechin in the dried gambier extract was carried out with TLC method. The molecular weight and content of catechin of dried gambier extract was determined by analyzing its mass spectra and spectrophotometer, respectively. The free radical scavenging activity of catechin of the resultant extracts was measured by using 1,1diphenyl-2-picrylhydrazyl (DPPH) as stable free radical compound. The anti-hyperglycemic activity of catechin of ethyl acetate extracts was analyzed as α-glucosidase inhibitor. The result showed that various gambiers available in the market are very active as antioxidant, indicated by IC 50 of catechin of ethyl acetate extracts which were 4.6 to 18.2 μg/mL for DPPH inhibition. The IC 50 for α-glucosidase inhibition is ranged from 40.45 to 52.43 μg/mL, so they can be classified as anti-diabetic.
Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attribut... more Gambier (Uncaria gambier) is known to have antioxidant properties, and some studies have attributed it to the presence of polyphenols such as catechin. The objective of this study is to investigate the potential of various gambiers available in Indonesian market as a scavenger of reactive free radicals and evaluate its anti-hyperglycemic activity as αglucosidase inhibitor. Isolation of catechin was done by extraction method with technical grade of ethyl acetate as solvent. Analysis of catechin in the dried gambier extract was carried out with TLC method. The molecular weight and content of catechin of dried gambier extract was determined by analyzing its mass spectra and spectrophotometer, respectively. The free radical scavenging activity of catechin of the resultant extracts was measured by using 1,1diphenyl-2-picrylhydrazyl (DPPH) as stable free radical compound. The anti-hyperglycemic activity of catechin of ethyl acetate extracts was analyzed as α-glucosidase inhibitor. The result showed that various gambiers available in the market are very active as antioxidant, indicated by IC 50 of catechin of ethyl acetate extracts which were 4.6 to 18.2 μg/mL for DPPH inhibition. The IC 50 for α-glucosidase inhibition is ranged from 40.45 to 52.43 μg/mL, so they can be classified as anti-diabetic.