André Dias De Azevedo Neto (original) (raw)

Papers by André Dias De Azevedo Neto

Trans-form-acao, 2010

Five biochemical descriptors were assessed in cotton plants infected with the ramulosis-causing f... more Five biochemical descriptors were assessed in cotton plants infected with the ramulosis-causing fungus in order to detect differences associated with the infection. Seeds from four cotton cultivars with different levels of resistance were sown in plastic containers in greenhouse conditions. At 20 days after emergence, plants were inoculated with a suspension at 1 x 106 conidia/mL of Colletotrichum gossypii var. cephalosporioides. Leaves were collected at 3, 15 and 30 days after inoculation and free proline, catalase, peroxidase, soluble carbohydrates and total proteins were determined. Proline, peroxidase and catalase levels discriminated the reaction of infected plants. These descriptors changed soon after infection and the change was more evident in the resistant BRS Antares variety. Free proline and catalase descriptors can be used in the identification of ramulosis-resistant cultivars in cotton breeding programs.

Journal of Plant Interactions, 2010

The effects of drought on water relations, gas exchanges, solutes accumulation, and catalase (CAT... more The effects of drought on water relations, gas exchanges, solutes accumulation, and catalase (CAT), ascorbato peroxidase (APX), and guaiacol peroxidase (GPX) activities were studied in five Arachis genotypes, grown under control or withholding water conditions. Drought stress reduced plant growth of all genotypes; the genotypes A. duranensis 7988 and A. stenosperma SV2411 being characterized as the most drought-sensitive and A. ipaensis as the most drought-tolerant. Data of transpiration and stomatal conductance confirmed the findings that A. ipaensis was more tolerant to drought conditions. Water deficit increased organic solutes content and reduced leaf water potential in all genotypes. The data suggest that solutes accumulation in roots may, at least in part, explain the greater tolerance of A. ipaensis to drought stress. CAT activity showed a significant increase in stressed leaves of sensitive genotypes. APX and GPX activities either increased or were not affected by drought in leaves of all genotypes.

Acta Botanica Brasilica, 2003

The aim of this work was to evaluate the estomatal behavior and the leaf water potential in Mimos... more The aim of this work was to evaluate the estomatal behavior and the leaf water potential in Mimosa caesalpiniifolia, Enterolobium contortisiliquum and Tabebuia aurea young plants cultivated under water stress. This work was performed in greenhouse of the Laboratório de Fisiologia Vegetal of the Universidade Federal Rural de Pernambuco, Brazil. Seedlings were submitted to two water treatment (100% and 50% pot capacity). On the 5th, 7th, 13th and 22th days of water treatments, the transpiration (E), diffusive resistance (Rs), leaf temperature (Tfol), air temperature (Tar), relative air humidity (UR), photossynthetically active radiation (PAR) and vapour pressure deficit (DPV) were evaluated at 7, 9, 12, 15h. After 30th days the leaf water potential (Yw) was measured, between 9-10h. The highest values of E were registered between 9-12h for all the species, although the stressed plants presented inferior values compared to the control plants. The opposite was verified for the Rs, however the differences in the magnitude and values among species and hours of evaluated were observed. The stomatal behavior of the stressed plants of E. contortisiliquum was influenced by the Tar and the M. caesalpiniifolia was influenced by the UR and PAR. The Yw decreased with the placing of stress, with reduction as much as 155%. The leaf temperature did not show a good indicator on the effects of water stress on the species studied.

Environmental and Experimental Botany, 2008

Soil salinity affects plant growth and development due to harmful ion effects and water stress ca... more Soil salinity affects plant growth and development due to harmful ion effects and water stress caused by reduced osmotic potential in the soil solution. In order to evaluate the effects of salt stress in young umbu plants, research was performed in green house conditions at the Laboratory of Plant Physiology at Federal Rural University of Pernambuco, Brazil. Growth, stomatal behaviour, water relations, and both inorganic and organic solutes were studied aiming for a better understanding of the responses of umbu plants to increasing salinity. Plants were grown in washed sand with Hoagland and Arnon nutrient solution with 0, 25, 50, 75, and 100 mM NaCl. Growth, leaf water potential, transpiration, and diffusive resistance were evaluated. Na+, K+, Cl−, soluble carbohydrates, and free amino acid contents were measured in several plant organs. Most variables were affected with salinity above 50 mM NaCl showing decreases in: number of leaves, plant height, stems diameter, and dry masses, and increases in root-to-shoot ratio. Reductions in ψpd were observed in plants grown under 75 and 100 mM NaCl. All salt levels above zero increased Na+ and Cl− contents in leaves. However, K+ content was not affected. Na+ and Cl− in stems and roots reached saturation in treatments above 50 mM NaCl. Organic solute accumulation in response to salt stress was not observed in umbu plants. These results suggest that umbu plants tolerate salt levels up to 50 mM NaCl without showing significant physio-morphological alterations.

Brazilian Journal of Plant Physiology, 2005

Two forage sorghum genotypes were studied: CSF18 (salt-sensitive) and CSF20 (salt-tolerant). Shoo... more Two forage sorghum genotypes were studied: CSF18 (salt-sensitive) and CSF20 (salt-tolerant). Shoot growth reduction as a result of salt stress was stronger in the salt sensitive genotype compared to the salt tolerant one. When the two genotypes were subjected to salt stress (75 mM NaCl) no significant change in lipid peroxidation was observed. However, salt stress induced increases in superoxide dismutase and catalase activities in both genotypes. These salt-induced increases were higher in the salt-tolerant genotype. Peroxidase activity was differentially affected by salt stress in the two genotypes. The activities of these peroxidases were decreased by salt stress in the salt-sensitive genotype and increased in the salt-tolerant genotype. In addition, the activity ratio between the superoxide dismutase and the H2O2-scavenging enzymes was higher in the salt-sensitive genotype. The results obtained support the hypothesis that the higher efficiency of the antioxidant-enzymatic system of the CSF20 genotype could be considered as one of the factors responsible for its tolerance to salt stress. Therefore, it is suggested that the ratio between superoxide dismutase and H2O2-scavenging enzyme activities could be used as a working hypothesis for a biochemical marker for salt tolerance in sorghum.

Journal of Plant Physiology, 2005

The effect of exogenously applied H2O2 on salt stress acclimation was studied with regard to plan... more The effect of exogenously applied H2O2 on salt stress acclimation was studied with regard to plant growth, lipid peroxidation, and activity of antioxidative enzymes in leaves and roots of a salt-sensitive maize genotype. Pre-treatment by addition of 1 μM H2O2 to the hydroponic solution for 2 days induced an increase in salt tolerance during subsequent exposure to salt stress. This was evidenced by plant growth, lipid peroxidation and antioxidative enzymes measurements. In both leaves and roots the variations in lipid peroxidation and antioxidative enzymes (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase, and catalase) activities of both acclimated and unacclimated plants, suggest that differences in the antioxidative enzyme activities may, at least in part, explain the increased tolerance of acclimated plants to salt stress, and that H2O2 metabolism is involved as signal in the processes of maize salt acclimation.

Journal of Plant Interactions, 2009

The effects of salt stress on the contents of organic solutes and on the pattern of free amino ac... more The effects of salt stress on the contents of organic solutes and on the pattern of free amino acids were studied in leaves and roots of two maize genotypes, BR5033 (salt-tolerant) and BR5011 (salt-sensitive). In leaves and roots of salt-stressed plants, soluble amino-N increased with time when compared to the controls. Salt stress increased the soluble protein content only in leaves of BR5011. Salinity increased the content of the majority of the free amino acids in leaves and roots of genotypes studied. Results suggest the hypothesis of disturbances in translocation of N-containing compounds from shoot to root in the salt-sensitive genotype. Results also suggest that the accumulation of organic solutes, mainly in roots of BR5033, may have an important role in the tolerance of this genotype to salt stress.

Brazilian Journal of Plant Physiology, 2004

Seeds from eight different maize genotypes (BR3123, BR5004, BR5011, BR5026, BR5033, CMS50, D766 a... more Seeds from eight different maize genotypes (BR3123, BR5004, BR5011, BR5026, BR5033, CMS50, D766 and ICI8447) were sown in vermiculite, and after germination they were transplanted into nutrient solution or nutrient solution containing 100 mmol.L-1 of NaCl and placed in a greenhouse. During the experimental period plant growth (dry matter, shoot to root dry mass ratio, leaf area, relative growth rate, and net assimilation rate), leaf temperature, stomatal conductance, transpiration, predawn water potential, sodium, potassium, soluble amino acids and soluble carbohydrate contents were determined in both control and salt stressed plants of all genotypes studied. Salt stress reduced plant growth of all genotypes but the genotypes BR5033 and BR5011 were characterized as the most salt-tolerant and salt-sensitive, respectively. Stomatal response of the salt-tolerant genotype was not affected by salinity. Among the studied parameters, shoot to root dry mass ratio, leaf sodium content and leaf soluble organic solute content showed no relation with salt tolerance, i.e., they could not be considered as good morpho-physiological markers for maize salt tolerance. In contrast, sodium and soluble organic solutes accumulation in the roots as a result of salt stress appeared to play an important role in the acclimation to salt stress of the maize genotypes studied, suggesting that they could be used as physiological markers during the screening for salt tolerance.

Environmental and Experimental Botany, 2006

The effects of salt stress on the activity of antioxidative enzymes and lipid peroxidation were s... more The effects of salt stress on the activity of antioxidative enzymes and lipid peroxidation were studied in leaves and roots of two maize genotypes, BR5033 (salt-tolerant) and BR5011 (salt-sensitive), grown under control (nutrient solution) or salt stress (nutrient solution containing 100 mM NaCl) conditions. Leaves and roots of control and salt-stressed plants were harvested at various times starting 1 day prior to initiating the salt treatment. In leaves of salt-stressed plants, superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and glutathione reductase (GR) activities increased with time when compared to the controls. The increase in enzyme activities was more pronounced in the salt-tolerant than in the salt-sensitive genotype. Salt stress had no significant effect on catalase (CAT) activity in the salt-tolerant, but it was reduced significantly in the salt-sensitive genotype. In salt-stressed roots of the salt-tolerant genotype, SOD and CAT activities decreased and APX, GPX and GR activities remained unchanged in comparison with the control. In roots of the salt-sensitive genotype, salinity reduced the activity of all studied enzymes. The data show that CAT and GPX enzymes had the greatest H2O2 scavenger activity in both leaves and roots. Moreover, CAT, APX and GPX activities in conjunction with SOD seem to play an essential protective role in the scavenging processes. Lipid peroxidation was enhanced only in salt-stressed leaves of the salt-sensitive genotype. These results indicate that oxidative stress may play an important role in salt-stressed maize plants and that the greater protection of BR5033 leaves and roots from salt-induced oxidative damage results, at least in part, through the maintenance and/or increase of the activity of antioxidant enzymes.