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Papers by Brunelle Andrade

Journal of Apicultural Research, 2020

Melipona scutellaris Latreille, 1811 is of economic importance for local beekeepers, besides its ... more Melipona scutellaris Latreille, 1811 is of economic importance for local beekeepers, besides its relevance in the pollination of native plant species of the Brazilian Atlantic forest. Currently, data on the use of floral resources by Meliponini colonies are scarce, particularly in urban environments. We evaluated the botanical origin, the microbiological and physicochemical characteristics of pollen stored by M. scutellaris in colonies in an urban environment. The samples (n = 44) were obtained from the metropolitan region of Salvador, Bahia, Brazil, a region of intense urban and industrial activities. We identified 52 pollen types belonging to 21 botanical families. The botanical families Fabaceae, Myrtaceae, and Anacardiaceae represented most pollen types. Aerobic psychrotrophic bacteria, Bacillus spp., molds and yeasts, fecal coliforms, Escherichia coli, Staphylococcus aureus, sulfite-reducing Clostridium, and Salmonella spp. were not found in the samples. We identified and quant...

This study aims to morphologically characterize pollen grains of the Passifloraceae family, occur... more This study aims to morphologically characterize pollen grains of the Passifloraceae family, occurring in the Serra Branca/Raso da Catarina Environmental Protection Area (EPA), Jeremoabo, Bahia, Brazil, in order to complement the palynological and taxonomic survey carried out in the area, as well as to provide subsidies for developing the EPA management plan. Flower buds were collected from herborized material deposited in the HUNEB Herbarium – Paulo Afonso Collection. The pollen material was acetolyzed, measured and described under optical and scanning electron microscopy (SEM). In the Raso da Catarina Ecoregion, the Passifloraceae family is comprised by four species from the genus Passiflora: P. cincinnata Mast., P. foetida L., P. luetzelburgii Harms, and P. setacea DC. All species had large size, 6-syncolpate pollen grains; paired apertures, with three pontopercula and three pseudopercula, reticulate exine with bacula or granules within the large sinuous reticula. The obtained res...

Melipona scutellaris (Apidae, Meliponini) is one of the main species of stingless bees used in be... more Melipona scutellaris (Apidae, Meliponini) is one of the main species of stingless bees used in beekeeping in the Northeast of Brazil. We examined the honey from M. scutellaris as an indicator to evaluate the levels of metals at sampling sites subject to a broad spectrum of environmental pollutants. The collections were carried out in the urban-industrial area of Salvador, Bahia and the metropolitan region. Samples (n= 58) were submitted to the nitroperchloric digestion procedure. We used the inductively coupled plasma optical emission spectrometry technique (ICP OES) to determine the concentration of metals (Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb and Zn) in the samples. The studied metals were detected among the samples, which presented tolerable levels according to current Brazilian legislation and recommendations from the World Health Organization (WHO), except for Cr, which presented mean values higher than the threshold for all sampling sites. The detection of the analyzed metals in...

Grana

Abstract Pollen in honey reflects its botanical origin and melissopalynology is used to identify ... more Abstract Pollen in honey reflects its botanical origin and melissopalynology is used to identify origin, type, and quantities of pollen grains of the botanical species visited by bees. This study aimed to identify the pollen spectrum of honeys from Apis mellifera and stingless bees produced in the semi-arid region of Bahia, Brazil. We analysed 78 honey samples, which were submitted to the acetolysis process for identification and quantification of pollen types. Fabaceae, Asteraceae and Euphorbiaceae were the most predominant families in pollen types. For Fabaceae, the most representative pollen types were Chamaecrista 1, Mimosa caesalpiniifolia, Mimosa pudica, Mimosa tenuiflora, Prosopis and Senna. The results indicate that the flora explored by the bees to collect nectar is diverse in the semi-arid region of Bahia and the honeys analysed were classified as multifloral.

Sociobiology

The floristic composition of an environment is important to ensure the trophic niche of bee speci... more The floristic composition of an environment is important to ensure the trophic niche of bee species. Melipona scutellaris Latreille, is a typical stingless bee of Atlantic rainforest sites in northeastern Brazil, a region widely established in meliponaries for honey and pollen production. M. scutellaris is reared (meliponiculture) in rural and urban areas, where the species depends on the availability of different plants for nectar and pollen collection. In this study, we estimated food niche width, equitativity, and similarity between different colonies of M. scutellaris in highly urbanized and industrialized sites of the Metropolitan Region of Salvador, Bahia State, Brazil. We analyzed pollen spectrum of 58 honey samples from six meliponaries, during 12 months. We identified 111 pollen types distributed in 28 plant families. The Fabaceae family showed the highest diversity in pollen types (33.33% of the total) and Mimosa caesalpiniifolia was the most frequent pollen type, found in...

Sociobiology, 2020

Transportation to long distances and handling of colonies can affect development and survival con... more Transportation to long distances and handling of colonies can affect development and survival conditions of bees. Our study investigated the stress intensity of individuals of Melipona scutellaris Latreille, 1811, due to transportation of colonies to long distances, within the natural range of the species. We used 746 bee workers. The right and left forewings were removed and measured using 15 landmarks in vein insertions. Individuals were divided into four groups: (1) workers collected at the origin site, (2) workers emerged at the place of destination in pupal stage during transportation, (3) workers emerged at the destination site in the 3rd instar of larval stage during transportation, and (4) workers collected after three months of colony establishment at the destination site. The Procrustes ANOVA showed significant results as well as the presence of Fluctuating Asymmetry (FA) in all treatments for the shape of wings (P<0.01). However, in the comparison of groups using the O...

Journal of Apicultural Research, 2020

Melipona scutellaris Latreille, 1811 is of economic importance for local beekeepers, besides its ... more Melipona scutellaris Latreille, 1811 is of economic importance for local beekeepers, besides its relevance in the pollination of native plant species of the Brazilian Atlantic forest. Currently, data on the use of floral resources by Meliponini colonies are scarce, particularly in urban environments. We evaluated the botanical origin, the microbiological and physicochemical characteristics of pollen stored by M. scutellaris in colonies in an urban environment. The samples (n = 44) were obtained from the metropolitan region of Salvador, Bahia, Brazil, a region of intense urban and industrial activities. We identified 52 pollen types belonging to 21 botanical families. The botanical families Fabaceae, Myrtaceae, and Anacardiaceae represented most pollen types. Aerobic psychrotrophic bacteria, Bacillus spp., molds and yeasts, fecal coliforms, Escherichia coli, Staphylococcus aureus, sulfite-reducing Clostridium, and Salmonella spp. were not found in the samples. We identified and quant...

This study aims to morphologically characterize pollen grains of the Passifloraceae family, occur... more This study aims to morphologically characterize pollen grains of the Passifloraceae family, occurring in the Serra Branca/Raso da Catarina Environmental Protection Area (EPA), Jeremoabo, Bahia, Brazil, in order to complement the palynological and taxonomic survey carried out in the area, as well as to provide subsidies for developing the EPA management plan. Flower buds were collected from herborized material deposited in the HUNEB Herbarium – Paulo Afonso Collection. The pollen material was acetolyzed, measured and described under optical and scanning electron microscopy (SEM). In the Raso da Catarina Ecoregion, the Passifloraceae family is comprised by four species from the genus Passiflora: P. cincinnata Mast., P. foetida L., P. luetzelburgii Harms, and P. setacea DC. All species had large size, 6-syncolpate pollen grains; paired apertures, with three pontopercula and three pseudopercula, reticulate exine with bacula or granules within the large sinuous reticula. The obtained res...

Melipona scutellaris (Apidae, Meliponini) is one of the main species of stingless bees used in be... more Melipona scutellaris (Apidae, Meliponini) is one of the main species of stingless bees used in beekeeping in the Northeast of Brazil. We examined the honey from M. scutellaris as an indicator to evaluate the levels of metals at sampling sites subject to a broad spectrum of environmental pollutants. The collections were carried out in the urban-industrial area of Salvador, Bahia and the metropolitan region. Samples (n= 58) were submitted to the nitroperchloric digestion procedure. We used the inductively coupled plasma optical emission spectrometry technique (ICP OES) to determine the concentration of metals (Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb and Zn) in the samples. The studied metals were detected among the samples, which presented tolerable levels according to current Brazilian legislation and recommendations from the World Health Organization (WHO), except for Cr, which presented mean values higher than the threshold for all sampling sites. The detection of the analyzed metals in...

Grana

Abstract Pollen in honey reflects its botanical origin and melissopalynology is used to identify ... more Abstract Pollen in honey reflects its botanical origin and melissopalynology is used to identify origin, type, and quantities of pollen grains of the botanical species visited by bees. This study aimed to identify the pollen spectrum of honeys from Apis mellifera and stingless bees produced in the semi-arid region of Bahia, Brazil. We analysed 78 honey samples, which were submitted to the acetolysis process for identification and quantification of pollen types. Fabaceae, Asteraceae and Euphorbiaceae were the most predominant families in pollen types. For Fabaceae, the most representative pollen types were Chamaecrista 1, Mimosa caesalpiniifolia, Mimosa pudica, Mimosa tenuiflora, Prosopis and Senna. The results indicate that the flora explored by the bees to collect nectar is diverse in the semi-arid region of Bahia and the honeys analysed were classified as multifloral.

Sociobiology

The floristic composition of an environment is important to ensure the trophic niche of bee speci... more The floristic composition of an environment is important to ensure the trophic niche of bee species. Melipona scutellaris Latreille, is a typical stingless bee of Atlantic rainforest sites in northeastern Brazil, a region widely established in meliponaries for honey and pollen production. M. scutellaris is reared (meliponiculture) in rural and urban areas, where the species depends on the availability of different plants for nectar and pollen collection. In this study, we estimated food niche width, equitativity, and similarity between different colonies of M. scutellaris in highly urbanized and industrialized sites of the Metropolitan Region of Salvador, Bahia State, Brazil. We analyzed pollen spectrum of 58 honey samples from six meliponaries, during 12 months. We identified 111 pollen types distributed in 28 plant families. The Fabaceae family showed the highest diversity in pollen types (33.33% of the total) and Mimosa caesalpiniifolia was the most frequent pollen type, found in...

Sociobiology, 2020

Transportation to long distances and handling of colonies can affect development and survival con... more Transportation to long distances and handling of colonies can affect development and survival conditions of bees. Our study investigated the stress intensity of individuals of Melipona scutellaris Latreille, 1811, due to transportation of colonies to long distances, within the natural range of the species. We used 746 bee workers. The right and left forewings were removed and measured using 15 landmarks in vein insertions. Individuals were divided into four groups: (1) workers collected at the origin site, (2) workers emerged at the place of destination in pupal stage during transportation, (3) workers emerged at the destination site in the 3rd instar of larval stage during transportation, and (4) workers collected after three months of colony establishment at the destination site. The Procrustes ANOVA showed significant results as well as the presence of Fluctuating Asymmetry (FA) in all treatments for the shape of wings (P<0.01). However, in the comparison of groups using the O...