Andre Berville - Academia.edu (original) (raw)

Papers by Andre Berville

Theoretical and Applied Genetics, 1996

Cloning and analysis of a 6.8-kb rDNA intergenic spacer region of the European ash (Fraxinus exce... more Cloning and analysis of a 6.8-kb rDNA intergenic spacer region of the European ash (Fraxinus excelsiorL)

Theoretical and Applied Genetics, 1995

The ribosomal DNA repeat units of two closely related species of the genus Fraxinus, F. excelsior... more The ribosomal DNA repeat units of two closely related species of the genus Fraxinus, F. excelsior and F. oxyphylla, were characterized. The physical maps were constructed from DNA digested with BamHI, EcoRI, EcoRV and SacI, and hybridized with three heterologous probes. The presence or the absence of an EcoRV restriction site in the 18s RNA gene characterizes two ribosomal DNA unit types found in both species and which coexist in all individuals. A third unit type appeared unique to all individuals of F, oxyphylla. It carries an EcoRI site in the intergenic spacer. Each type of unit displayed length variations. The rDNA unit length of F. excelsior and F. oxyphylla was determined with EcoRV restriction. It varied between 11 kb and 14.5 kb in F. excelsior and between 11.8 kb to 13.8 kb in F. oxyphylla. Using SacI restriction, at least ten spacer length variants were observed in F. excelsior, for which a detailed analysis was conducted. Each individual carries 2-4 length variants which vary by a 0.3-kb step multiple. This length variation was assigned to the intergenic spacer. By using the entire rDNA unit of flax as probe in combination with EcoRI restriction, each species can be unambiguously discriminated. The species-specific banding pattern was used to compare trees from a zone of sympatry between the two species. In some cases, a conflicting classification was obtained from morphological analysis and the use of the species-specific Communicated by P. M. A. Tigerstedt

Theoretical and Applied Genetics, 1992

Two highly repeated EcoRI (0.45 × 10(6)) and BamHI (0.17 × 10(6)) fragments per haploid genome we... more Two highly repeated EcoRI (0.45 × 10(6)) and BamHI (0.17 × 10(6)) fragments per haploid genome were found in sugar beet genomic DNA. Both fragments were located by 6% acrylamide-gel electrophoresis, purified and cloned in pUC18. Four of the inserts corresponding to each family were chosen for further study. Both fragment families display the main characteristics of the satellite DNA of animals and plants. The EcoRI and BamHI fragment families are arranged in long tandem arrays. Fragments of the EcoRI family (pBVE) were analyzed. They vary both in sequence and in length (158-160 nt) in comparison with the consensus sequence of 159nt. Both families are A-T rich; pBVE is 59% rich while pBVB is 69% rich. The BVESAT family is present in all the members of the section Vulgares. It is conserved in the section Procumbentes with 80% homology and the same length, but is not detectable in the Corollinae. The sequence variation rate and the variation in length (330±5 nt) are of the same order in comparison with those of the BVESAT family. However, the BVBSAT family is present in species of the section Vulgares only. As regards other plant satellite DNAs, the BVESAT family shares homology with Allium cepa satellite DNA, with three of the yeast centromeric sequences, and with three Arabidopsis thaliana sequences. The BVBSAT family is unique to the Vulgares and does not share any homology with other plant or animal satellite DNAs sequences so far.

Plant Molecular Biology, 1992

Molecular and Cellular Probes, 1991

Genome, 1992

The nuclear rDNA physical maps of Populus nigra, P. deltoides, P. trichocarpa, P. maximowiczii, a... more The nuclear rDNA physical maps of Populus nigra, P. deltoides, P. trichocarpa, P. maximowiczii, and P. alba have been built up for the restriction enzymes EcoRI, EcoRV, BamHI, PstI, and SacI. There is no HindIII site. A large variability appeared between the species in the intergenic spacer mainly for EcoRI and PstI. For several clones of each species two to three major unit types coexist in the genome, while several minor units as well as length variant units or new unit types have been found. The variability between the species is due to different major unit types, while the variability between clones in one species is due to the minor unit types. Every species carries several rDNA unit types either different in size (variable length unit) or variable in restriction sites (variable unit types). Four thousands copies of rDNA units were found in P. nigra, P. deltoides, and P. maximowiczii. The clones belonging to the same species carry the same major unit types but are different in ...

Current Genetics, 1985

Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Hel... more Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ultracentrifugation. 2. In polyacrylamide gel it is found that mitochondria from F cytoplasm contain a Low-Molecular-Weight (LMW) mt-DNA molecule of 1.45 Kilobase-pairs (kb) in addition to the major mt-DNA. On the contrary, mitochondria from S cytoplasm contain no LMW molecule. Treatments with DNAse, RNAse and Nuclease S1 show that this LMW mt-DNA molecule consists of a supercoiled circular DNA. The length and form of the LMW molecule are confirmed by electron microscopy observations. 3. The mt-DNAs from S and F cytoplasms have also been investigated using restriction endonuclease digestions (Sa11, Xho1, Bg11). The electrophoresis of the resulting fragments reveals several differences between the S and F cytoplasm. It is not known whether the differences observed between F and S mt-DNA are correlated with the cytoplasmic male sterility phenotype.

Correspondance : dosba@supagro.inra.frNational audienceLes olives et l'huile d'olive sont... more Correspondance : dosba@supagro.inra.frNational audienceLes olives et l'huile d'olive sont des produits bien connus de l'olivier. L'huile d'olive est un aliment emblématiqe dans toute la Méditerranée. Des recherches génétiques concernent sa traçabilité. Les signes de qualité se développent, notamment les AOC. Dans les temps anciens, elle est source d'éclairage. De tous temps, elle entre dans la fabrication de parfums, de savons renommés de Marseille, d'Alep... Elle est aussi riche en composants: acide oléique et polyphénols, qui diminuent les risques cardio-vasculaires ou l'ostéoporose. Des substances provenant de l'olivier et de l'huile d'olive entrent dans la composition de produits pharmaceutiques. La valorisation d'autres sous-produits est plus inattendue: dans les pays du sud de la Méditerranée, les grignons dont on a retiré les coques ainsi que des feuilles et des brindilles de l'arbre sont donnés comme aliments au bétail; les...

Correspondance : Berville@supagro.inra.frNational audienceL'olivier occupe une place ancestra... more Correspondance : Berville@supagro.inra.frNational audienceL'olivier occupe une place ancestrale dans les civilisation méditerranéennes. Bien au-delà, il accompagne les textes fondateurs de notre culture: bible, torah, talmud, coran, grands textes classiques grecs... Arbre des dieux, symbole de force, de longévité et de paix, il tend toujours vers la lumière. Il incarne une manière de vivr

National audienceAu sud du maroc existent des populations d'olea dont la morphologie est inte... more National audienceAu sud du maroc existent des populations d'olea dont la morphologie est intermédiaire entre la sous-espèce laperrinei et la sous-espèce europaea. Longtemps ignoré ou négligé, ce taxon d'olea a reçu des noms divers, dont celui de O. maroccana. Ce n'est que récemment, en 2001, que la morphologie et le marquage moléculaire ont permis de considérer cet olivier comme sous-espèce de l'olea europaea. La phénologie est introduite ici comme un critère nouveau. Les principales caractéristiques de cet olivier endémique du Maroc sont exposés. Les deux sous-espèces europaea et maroccana existent en sympatrie, ce qui est une situation originale et interroge sur leur évolution

Introduction : Data on olive cross and selfing studies were numerous... more Introduction : Data on olive cross and selfing studies were numerous and dispersed in literature. Varieties were list in lines, and each line correspond to one data – bag, pollen test, paternity tests, ...To compare data in bags standardization was achieved based on 100 hermaphroditic flowers. Materials and methods : Data from publications were recorded keeping the name of origin for each variety. Varieties in pair wise combinations in crosses enabled to decipher the S-allele pair (Breton and Bervillé 2012, Farinelli et al. 2014) leading to attribute the PASI pair. The G group (Saumitou-Laprade et al. 2017) was recorded from Mariotti et al. 2021. Results : The DSI pair and the PASI pair were introduced and sorting data by screening G1xG2 (1 for fruit) and G2xG1 (1) whereas by screening G1xG1 (0) and G2XG2 (0) show that all crosses display fruit. The DSSM reconciles DSI and PASI to explain Self-incompatibility. Selfing was shown appearing when crosses were 1-0 or 0-1, but not when 1-1, whereas some expected 0-0 combinations lead to fruit. Moreover, paternity tests (column embryo) revealed most of the time a father compatible in DSI , but incompatible in PASI, this is due to DS-D, that shows compatible pollen is insufficient. The database is useful to check whether the variety has already been studied for SI. It enables to check the homogeneity of cross data in literature.

La resistance du tournesol aux maladies fait l'objet de recherches depuis de longues annees. ... more La resistance du tournesol aux maladies fait l'objet de recherches depuis de longues annees. Les acquis de ces travaux et la complexite des questions a resoudre ont motive la mise en place du programme Cartisol dont on peut maintenant tirer un bilan. Ce programme a reuni des partenaires de la recherche publique, le CETIOM et des semenciers prives. Dans une premiere phase, une carte genetique du tournesol a ete etablie par marqueurs RFLP. Durant la deuxieme partie du programme, le partenariat mis en place a permis de mettre en evidence des QTL de resistance a Sclerotinia, et d'identifier des regions de la carte particulierement interessantes pour le marquage de QTL ou de genes de resistance a des pathogenes. Ce travail a abouti au depot d'un brevet et a la publication de plusieurs articles. Les acquis du programme constituent une base tres utile pour poursuivre la mise au point de nouveaux outils moleculaires d'aide a la selection.

Evolutionary Applications, 2018

This study was carried out to examine the validity of previous studies on the intercompatibility ... more This study was carried out to examine the validity of previous studies on the intercompatibility of olive and to compare the approach and techniques used for proposing the diallelic self-incompatibility system and the sporophytic self-incompatibility system. Analysis of the literature indicates that the mating system of the olive tree is a controversial issue and requires further studies to clearly and fully comprehend it. All possible approaches should be used to maximize reliability of the final conclusions on the olive mating system. 1 | INTRODUC TI ON Most of the studies on the intercompatibility of olive varieties carried out in recent decades have been reported to be debat

Theoretical and Applied Genetics, 2003

A genetic map was constructed with specific PCRs, DALPs and AFLPs using F8-generation sunflower r... more A genetic map was constructed with specific PCRs, DALPs and AFLPs using F8-generation sunflower recombinant inbred lines. RI lines generated from a F2 population of one cross between the two cultivated inbred lines HA89 (maintainer for Pet1 CMS) and LR4 (restorer for Pet1 CMS) were used. A total of 305 markers were located using seven sPCR, 64 DALP and 301 AFLP loci. They were generated with one, seven and 14 primer pairs, respectively. The map construction consisted of a two-step strategy using 6 and 3.1 LOD scores revealed by a simulation file. Mapped markers were assembled into 18 linkage groups covering 2,168.6 cM with an average of 6.1 cM. The distribution of DALPs and AFLPs revealed that both markers tagged different regions to enable covering most of the sunflower genome. This leads to the longest map published so far for sunflower.

Euphytica, 2016

Self-fertility is largely decreased and even prevented by various mechanisms because, broadly, it... more Self-fertility is largely decreased and even prevented by various mechanisms because, broadly, it causes inbreeding depression, although some species have retained self-reproduction regimes. Species of plants that display the self-incompatible sporophytic type of self-incompatibility may rarely self-pollinate. It is only possible in the absence of foreign compatible pollen. In the olive tree with a sporophytic mechanism, we will show that three co-dominant S-alleles R1, R3 and R5 do not lead to the same level of self-fertility. All varieties that carry R1 are less self-fertile than those that carry R5, whatever the other S-alleles, while those carrying R3 are intermediate. S-allele pair-wise combinations that differ by two or three levels of dominance, and not the other combinations allow selffertility, and moreover each S-allele R1, R3 and R5 decreases, maintains and enhances the self-fertility rate, respectively.

Oleoscope, 2003

Depuis plus de dix ans, l'INRA demonte les mecanismes d'action des facteurs genetiques im... more Depuis plus de dix ans, l'INRA demonte les mecanismes d'action des facteurs genetiques impliques dans la teneur en acide oleique des varietes de tournesol. Une longue recherche, a laquelle s'est associee la societe Monsanto en 1997, dont beneficient largement les programmes de selection.