Andrea A D Egito - Academia.edu (original) (raw)
Papers by Andrea A D Egito
Animals, 2019
This study aimed to identify bovine breeds through trichological morphology and morphometry and t... more This study aimed to identify bovine breeds through trichological morphology and morphometry and to validate this technique by comparing it with genetic characterization. Animals from Caracu, Curraleiro Pé-Duro, Nelore, and Bovino Pantaneiro breeds were studied. Morphological and morphometric analyses of the guard hairs were performed. The cuticular pattern was observed on the shaft and the medulla pattern on the shield of the samples. The cattle genetic characterization was accomplished using microsatellite markers. Statistical analyses were performed using R version 3.2.4 software. Pearson’s correlation test showed a high positive and significant correlation between the matrices generated by trichological and genetic analyses (r = 0,996 and p < 0.001). Trichological analysis is a useful method for cattle breed identification. Its potential for identifying other species of interest for animal production should be studied since it is a simple, low-cost, and non-invasive method.
Mitochondrial DNA Part A, 2016
The objective of this study was to identify, through the DNA barcode, fishable Siluriformes which... more The objective of this study was to identify, through the DNA barcode, fishable Siluriformes which were collected from the Paraguay River basin in Pantanal. It was analyzed for genetic distance calculation using the Kimura-two-model parameters and the dendrogram was builtusing the Neighbour-Joining algorithm. The average genetic distance within species, genus and families were 0.2%, 1.6% and 4.2%, respectively. These values were lower than those reported in studies from other continents, probably due to the recent radiation process undergone by Neotropical fish. The dendrogram revealed two possible cases of hybridization, one individual Pseudoplatystoma corruscans, it was not possible to identify whether it was a natural event or commercial production exhaust and other of Pimelodus cf. argenteus leading to the assumption that the aspects of reproductive isolation cannot be clearly defined. Besides, the populations of the species Hemisorubim platyrhynchos and e Platydora armatulus may be undergoing a substructuring process, with genetic differences 3% and 4%, respectively.
Raças bovinas nativas. Estrutura de populações. Conservação genética.
AFRICAN JOURNAL OF BIOTECHNOLOGY
The aim of this study was to assess genetic variation between a population of Pantaneira sheep in... more The aim of this study was to assess genetic variation between a population of Pantaneira sheep in the Brazilian state of Mato Grosso do Sul, and Creole sheep from the south of the country by molecular analysis of the ND5 gene in mitochondrial DNA. The analysis revealed the presence of 16 haplotypes with all Pantaneira sheep grouped together carrying a single haplotype, and there was no grouping with any of the Creole sheep. The F ST value was 0.44, indicating that there is a genetic difference between the two breeds, which may indicate that both breeds underwent differentiation.
ABSTRACT Ex situ conservation include the cryopreservation of genetic material: semen, oocytes, e... more ABSTRACT Ex situ conservation include the cryopreservation of genetic material: semen, oocytes, embryos, somatic cells and DNA (FAO, 1998). Although, it is not possible to regenerate whole animals from isolated DNA, it has been proved that DNA banking was useful in population genetic studies and epidemiological investigations. The Animal Genetics Laboratory (AGL) at Embrapa Genetic Resources and Biotechnology - Cenargen, Brasília - DF, Brazil, works with genetic characterization of animal populations that belong to the Brazilian conservation program. At the same time, a DNA Bank is being set up, which already has samples from several animals species. The DNA was extracted from cryopreserved leukocyte pellets or buffy coats. After extraction it was quantified and checked for its integrity and quality. Each specimen extracted was divided in at least two samples. One of them was used for characterization studies (-20°C) while the other was stored at -80°C at the DNA bank. All animals that were collected received a code number and were catalogued in a data bank where all the available information was registered. The refinement of molecular biology techniques increase the usefulness of banked material that will provide a ready reservoir of valuable scientific information. At this time, the AGL DNA bank has specimens of several breeds of six domestic species. Most of these breeds are in risk of extinction and are involved in the Conservation Program of Embrapa/Cenargen. DNA banking is proving to be useful for characterization of domestic animal populations that are in Brazilian conservation program. In near future, it might be the method of choice when many representatives of a breed are to be stored.
Livestock Science, 2009
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately. This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.
Pesquisa Agropecuária Brasileira
Resumo -O objetivo deste trabalho foi a caracterização genética da raça bovina Crioulo Lageano po... more Resumo -O objetivo deste trabalho foi a caracterização genética da raça bovina Crioulo Lageano por marcadores RAPD em comparação com as raças Holandesa e Nelore. Foram selecionados 43 primers, que geraram 77 bandas polimórficas. Os animais foram distribuídos em cinco subgrupos de Crioulo Lageano (I a V), e um subgrupo em cada uma das raças Holandesa (VI) e Nelore (VII). A maior parte da variância genética total (65,05%) foi causada pela diferença de indivíduos dentro dos grupos, e o restante pelas diferenças entre grupos. A análise conjunta dos grupos I a V apresentou variabilidade genética entre grupos de 25,28% e dentro dos grupos de 74,72%. A diversidade gênica vem se mantendo ao longo das gerações no núcleo de conservação do Crioulo Lageano. A raça Holandesa apresentou a menor diversidade gênica (0,1204), e a Crioulo Lageano a maior (0,3154). A maior distância genética (0,3747) foi entre as raças Nelore e Holandesa. Os grupos de Crioulo Lageano apresentaram diferenças entre si e apenas alguns indivíduos de cada grupo posicionaram-se junto a outros grupos. A técnica RAPD é capaz de estimar a distância genética entre raças ou populações e de auxiliar na escolha de indivíduos, visando aos trabalhos de conservação de recursos genéticos.
Pesquisa Agropecuaria Brasileira
Conservation and improvement strategies should be based on the association between genetic and ph... more Conservation and improvement strategies should be based on the association between genetic and phenotypic characteristics. The objective of this work was to characterize five native Brazilian cattle breeds (Caracu, Crioulo Lageano, Curraleiro, National Polled and Pantaneiro) and two commercial breeds (Holstein and Nellore) using RAPD technique to estimate genetic distances and variability between and within breeds. Genetic relationships were investigated using 22 primers which generated 122 polymorphic bands. Analysis of molecular variance indicated that most of the genetic variation lay among individuals within populations. The genetic variabilities between pairs of breeds were statistically significant. The smallest genetic divergence was between Crioulo Lageano and Curraleiro. The National Polled, although historically considered to be of Bos taurus aquitanicus origin, similar to the Caracu, was grouped together with the other breeds of Bos taurus ibericus origin. Generally, the individual breeds formed distinct clusters except the National Polled. The RAPD technique was capable to distinguish genetically between the breeds studied; the Caracu, Crioulo Lageano, Curraleiro and Pantaneiro may be considered distinct genetic entities thereby proving the uniqueness of the populations; the National Polled has not been able to re-establish itself after its decline in the 1950s, thereby losing its genetic identity.
Resumo -Com o intuito de testar a origem da raça Santa Inês, bem como avaliar as unicidades das p... more Resumo -Com o intuito de testar a origem da raça Santa Inês, bem como avaliar as unicidades das populações das mais importantes raças naturalizadas brasileiras de ovinos deslanados foi feito um estudo dos padrões de semelhança molecular obtidos a partir de marcadores RAPD-PCR. Foram utilizados 238 indivíduos provenientes dos Estados de Goiás, Sergipe, Bahia, Distrito Federal, e Ceará distribuídos entre as raças Santa Inês, Bergamácia, Rabo Largo, Morada Nova e Somalis. Ao final da triagem de 140 primers foram selecionados 54 loci a partir de 19 primers. As análises intra-raciais mostraram a presença de marcadores específicos entre as raças analisadas. Nas análises inter-raciais, todas as raças foram significativamente diferentes entre si (Teste Exato de Fisher p<0,01). Paralelamente, foi observado pela análise de variância molecular (AMOVA), que apenas 14,92% da variação total (p<0,01) foi em razão de diferenças inter-raciais. A análise de agrupamento mostrou que a Raça Santa Inês ficou mais próxima da Bergamácia (97% bootstrapping) e que a raça Rabo Largo teria maior similaridade a este grupo do que a raça Morada Nova e Somalis (81% bootstrapping). Os resultados sugerem que medidas para conservação e manejo já podem ser planejadas e que novos estudos abrangendo tanto loci de microssatélites e mitocondriais como a inclusão de novas raças lanadas poderão fornecer uma melhor base para confirmar a origem da raça Santa Inês.
Pesquisa Agropecuaria Brasileira
The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep... more The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep breeds and to determine the origin of the Santa Inês breed. Molecular similarity was determined using Randomly Amplified Polymorphic DNA -Polymerase Chain Reaction markers in 238 individuals from five naturalized sheep breeds: Santa Inês (48 animals), Rabo Largo , Somali (48), Morada Nova and Bergamasca , collected in Goiás, Sergipe, Bahia, and Ceará States as well as in the Federal District. Fifty-four loci were selected from 19 primers, after a pilot test using 140 primers. Qualitative analyses indicate diagnostic markers for all breeds. All breeds were significantly different from each other. Interbreed differences were explained by 14.92% of the total variation. Santa Inês clustered with Bergamasca (97% bootstrap) and with Rabo Largo, composing the third member of the group (81% bootstrap) while Morada Nova and Somali breeds clustered separately. Each breed should be considered as a separate management and conservation unit, and special care should be taken with Rabo Largo, Morada Nova and Somali breeds, represented by small herds in Brazil.
Pesquisa Agropecuária Brasileira
The objective of this study was to verify the genetic diversity between and within seven populati... more The objective of this study was to verify the genetic diversity between and within seven populations of Moxoto goat (n = 264) from the States of Pernambuco, Paraiba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxoto, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, white 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Flor...
investigación y otra almacenada en el Banco de ADN, mantenida en congelador a -80 o C. El Banco c... more investigación y otra almacenada en el Banco de ADN, mantenida en congelador a -80 o C. El Banco cuenta con 11.118 muestras de ADN de vacunos, equinos, mulas, búfalos, cabras y ovejas, así como aves y algunas especies productivas de tortugas, peces y roedores. El Banco contiene material para la caracterización molecular de las razas y la identificación de genes de interés. Su formación se debe al esfuerzo de investigadores y colaboradores, lo que refuerza la importancia de alianzas en la Conservación y Uso de los Recursos Genéticos Animales en Brasil. El futuro del banco será la utilización de herramientas estadísticas para comprobar y ajustar su alcance nacional y guía nuevas recogidas en hatos relacionados con programas de pre-mejora y mejora, además de dirigir la selección de donantes de Banco de Germoplasma.
As the aim of investigating the population structure of Brazilian bovine locally adapted breeds, ... more As the aim of investigating the population structure of Brazilian bovine locally adapted breeds, 237 individuals from Caracu (n = 50), Creole Lageana (n = 39), Curraleira (n = 50), Mocha Nacional (n = 50) and Pantaneira breeds (n = 48) were genotyped with 28 microsatellite loci chosen from lists of FAO / ISAG and BIOBOVIS project. Indices of genetic diversity were calculated by FSAT program and the genetic population structure was obtained based on Bayesian analysis implemented by the STRUCTURE program. There was a significant genetic differentiation (p <0.05) between breeds studied, the percentage of variation among populations was 4.64%. In general the expected heterozygosity was higher than observed heterozygosity (0.751 vs 0.696). The Creole Lageana breed showed the highest allelic richness (8.9) while the lowest was observed in the Caracu breed (7.07). The greatest FIS was observed in Curraleira (0.106) and the smallest in the Mocho National (0.035). By the Bayesian analysis...
Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. 23 p. – (Documentos / Embrapa Rec... more Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. 23 p. – (Documentos / Embrapa Recursos Genéticos e Biotecnologia, ISSN 0102-0110 ; 166)
Theriogenology, 2002
Brazil has various species of domestic animals, which developed from breeds brought by the Portug... more Brazil has various species of domestic animals, which developed from breeds brought by the Portuguese settlers soon after their discovery. For five centuries, these breeds have been subjected to natural selection in specific environments. Today, they present characteristics adapted to the specific Brazilian environmental conditions. These breeds developed in Brazil are known as "Crioulo," "local," or naturalized. From the beginning of the 20th century, some exotic breeds, selected in temperate regions, have begun to be imported. Although more productive, these breeds do not have adaptive traits, such as resistance to disease and parasites found in breeds considered to be "native." Even so, little by little, they replaced the native breeds, to such an extent that the latter are in danger of extinction. In 1983, to avoid the loss of this important genetic material, the National Research Center for Genetic Resources and Biotechnology (Cenargen) of the Brazilian Agricultural Research Corporation (Embrapa) decided to include conservation of animal genetic resources in its research program Conservation and Utilization of Genetic Resources. Until this time, they were only concerned with conservation of native plants. Conservation has been carried out by various research centers of Embrapa, universities, state research corporations, and private farmers, with a single coordinator at the national level, Cenargen. Specifically, conservation is being carried out by conservation nuclei, which are specific herds in which the animals are being conserved, situated in the habitats where the animals have been subjected to natural selection. This involves storage of semen and embryos from cattle, horses, buffaloes, donkeys, goats, sheep, and pigs. The Brazilian Animal Germplasm Bank is kept at Cenargen, which is responsible for the storage of semen and embryos of various breeds of domestic animals threatened with extinction, where almost 45,000 doses of semen and more than 200 embryos exist presently. An important challenge for this program is to make the different segments of society realize the importance of the conservation of animal genetic resources.
Corumbá: Embrapa Pantanal, 2003. 29 p.; 21 cm (Documentos / Embrapa Pantanal ISSN 1517-1973; 55).
O objetivo deste trabalho foi caracterizar uma população de animais da raça Nelore Variedade Verm... more O objetivo deste trabalho foi caracterizar uma população de animais da raça Nelore Variedade Vermelha visando estimar parâmetros populacionais e sua composição genética mediante comparação com raças localmente adaptadas e com a raça Nelore Padrão. Foram genotipados nove locos microssatélites em 192 indivíduos das seguintes raças: Caracu (40), Curraleiro (40), Pantaneiro (40), Nelore Padrão (40) e Vermelho (32). Todas as raças exibiram uma redução significativa na heterozigosidade observada (Ho), quando comparada com a heterozigosidade esperada (He) devido a acasalamentos que não ocorreram ao acaso dentro das populações. A raça Nelore Vermelha apresentou valores mais altos de heterozigosidade esperada e observada (0,756 e 0,707, respectivamente). Os resultados obtidos mostraram que existe variabilidade genética significativa em todos os grupos estudados (> 0,68). A relação entre os grupamentos genéticos foi analisada. As populações Nelore Padrão e Variedade Vermelha (Bos indicus) ficaram agrupadas distintamente das demais raças Bos taurus (Caracu, Curraleiro e Pantaneiro). As estimativas de diferenciação genética aos pares indicam que todas as populações estudadas podem ser consideradas entidades geneticamente independentes (p<0,01), embora não tenha sido possível distinguir, por método de agrupamento Bayesiano, as diferentes linhagens da raça Nelore. Palavras-chave: diversidade genética, genética de populações, STR, Structure
Objetivou-se analisar a frequência gênica de polimorfismos dos genes estearoil-CoA dessaturase (S... more Objetivou-se analisar a frequência gênica de polimorfismos dos genes estearoil-CoA dessaturase (SCD1) e ácido graxo sintase (FASN) em diferentes raças taurinas localmente adaptadas. Os animais foram genotipados para os polimorfismos g.16024 A>G e g.16039 T>C do gene FASN pela técnica de PCR-RFLP e os polimorfismos SCD1-702 G>A e SCD1-762 C>T do gene SCD1 por sequenciamento. As frequências do haplótipo favorável TW do gene FASN foi maior nas raças Crioula Lageana e Caracu em relação às demais. Em relação ao gene SCD1, para o polimorfismo SCD1-702 A>G, as raças Curraleira, Crioula Lageana, e Pantaneira apresentaram menor frequência do alelo favorável A (0,05, 0,01 e 0,11) e para o polimorfismo SCD1-762 C>T, apresentam frequências mais altas do alelo favorável T de 0,39, 0,45 e 0,68, respectivamente. Devido a essas divergências entre as freqüências, sugeriu-se a validação desses SNPs com dados fenotípicos. Houve uma alta variabilidade genética dos polimorfismos avaliados nas raças localmente adaptadas, evidenciando seu potencial de uso em programas de melhoramento genético. Palavras-chave: bovinos, FASN, perfil de ácidos graxos, qualidade de carne, SCD1, SNP
Animals, 2019
This study aimed to identify bovine breeds through trichological morphology and morphometry and t... more This study aimed to identify bovine breeds through trichological morphology and morphometry and to validate this technique by comparing it with genetic characterization. Animals from Caracu, Curraleiro Pé-Duro, Nelore, and Bovino Pantaneiro breeds were studied. Morphological and morphometric analyses of the guard hairs were performed. The cuticular pattern was observed on the shaft and the medulla pattern on the shield of the samples. The cattle genetic characterization was accomplished using microsatellite markers. Statistical analyses were performed using R version 3.2.4 software. Pearson’s correlation test showed a high positive and significant correlation between the matrices generated by trichological and genetic analyses (r = 0,996 and p < 0.001). Trichological analysis is a useful method for cattle breed identification. Its potential for identifying other species of interest for animal production should be studied since it is a simple, low-cost, and non-invasive method.
Mitochondrial DNA Part A, 2016
The objective of this study was to identify, through the DNA barcode, fishable Siluriformes which... more The objective of this study was to identify, through the DNA barcode, fishable Siluriformes which were collected from the Paraguay River basin in Pantanal. It was analyzed for genetic distance calculation using the Kimura-two-model parameters and the dendrogram was builtusing the Neighbour-Joining algorithm. The average genetic distance within species, genus and families were 0.2%, 1.6% and 4.2%, respectively. These values were lower than those reported in studies from other continents, probably due to the recent radiation process undergone by Neotropical fish. The dendrogram revealed two possible cases of hybridization, one individual Pseudoplatystoma corruscans, it was not possible to identify whether it was a natural event or commercial production exhaust and other of Pimelodus cf. argenteus leading to the assumption that the aspects of reproductive isolation cannot be clearly defined. Besides, the populations of the species Hemisorubim platyrhynchos and e Platydora armatulus may be undergoing a substructuring process, with genetic differences 3% and 4%, respectively.
Raças bovinas nativas. Estrutura de populações. Conservação genética.
AFRICAN JOURNAL OF BIOTECHNOLOGY
The aim of this study was to assess genetic variation between a population of Pantaneira sheep in... more The aim of this study was to assess genetic variation between a population of Pantaneira sheep in the Brazilian state of Mato Grosso do Sul, and Creole sheep from the south of the country by molecular analysis of the ND5 gene in mitochondrial DNA. The analysis revealed the presence of 16 haplotypes with all Pantaneira sheep grouped together carrying a single haplotype, and there was no grouping with any of the Creole sheep. The F ST value was 0.44, indicating that there is a genetic difference between the two breeds, which may indicate that both breeds underwent differentiation.
ABSTRACT Ex situ conservation include the cryopreservation of genetic material: semen, oocytes, e... more ABSTRACT Ex situ conservation include the cryopreservation of genetic material: semen, oocytes, embryos, somatic cells and DNA (FAO, 1998). Although, it is not possible to regenerate whole animals from isolated DNA, it has been proved that DNA banking was useful in population genetic studies and epidemiological investigations. The Animal Genetics Laboratory (AGL) at Embrapa Genetic Resources and Biotechnology - Cenargen, Brasília - DF, Brazil, works with genetic characterization of animal populations that belong to the Brazilian conservation program. At the same time, a DNA Bank is being set up, which already has samples from several animals species. The DNA was extracted from cryopreserved leukocyte pellets or buffy coats. After extraction it was quantified and checked for its integrity and quality. Each specimen extracted was divided in at least two samples. One of them was used for characterization studies (-20°C) while the other was stored at -80°C at the DNA bank. All animals that were collected received a code number and were catalogued in a data bank where all the available information was registered. The refinement of molecular biology techniques increase the usefulness of banked material that will provide a ready reservoir of valuable scientific information. At this time, the AGL DNA bank has specimens of several breeds of six domestic species. Most of these breeds are in risk of extinction and are involved in the Conservation Program of Embrapa/Cenargen. DNA banking is proving to be useful for characterization of domestic animal populations that are in Brazilian conservation program. In near future, it might be the method of choice when many representatives of a breed are to be stored.
Livestock Science, 2009
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately. This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.
Pesquisa Agropecuária Brasileira
Resumo -O objetivo deste trabalho foi a caracterização genética da raça bovina Crioulo Lageano po... more Resumo -O objetivo deste trabalho foi a caracterização genética da raça bovina Crioulo Lageano por marcadores RAPD em comparação com as raças Holandesa e Nelore. Foram selecionados 43 primers, que geraram 77 bandas polimórficas. Os animais foram distribuídos em cinco subgrupos de Crioulo Lageano (I a V), e um subgrupo em cada uma das raças Holandesa (VI) e Nelore (VII). A maior parte da variância genética total (65,05%) foi causada pela diferença de indivíduos dentro dos grupos, e o restante pelas diferenças entre grupos. A análise conjunta dos grupos I a V apresentou variabilidade genética entre grupos de 25,28% e dentro dos grupos de 74,72%. A diversidade gênica vem se mantendo ao longo das gerações no núcleo de conservação do Crioulo Lageano. A raça Holandesa apresentou a menor diversidade gênica (0,1204), e a Crioulo Lageano a maior (0,3154). A maior distância genética (0,3747) foi entre as raças Nelore e Holandesa. Os grupos de Crioulo Lageano apresentaram diferenças entre si e apenas alguns indivíduos de cada grupo posicionaram-se junto a outros grupos. A técnica RAPD é capaz de estimar a distância genética entre raças ou populações e de auxiliar na escolha de indivíduos, visando aos trabalhos de conservação de recursos genéticos.
Pesquisa Agropecuaria Brasileira
Conservation and improvement strategies should be based on the association between genetic and ph... more Conservation and improvement strategies should be based on the association between genetic and phenotypic characteristics. The objective of this work was to characterize five native Brazilian cattle breeds (Caracu, Crioulo Lageano, Curraleiro, National Polled and Pantaneiro) and two commercial breeds (Holstein and Nellore) using RAPD technique to estimate genetic distances and variability between and within breeds. Genetic relationships were investigated using 22 primers which generated 122 polymorphic bands. Analysis of molecular variance indicated that most of the genetic variation lay among individuals within populations. The genetic variabilities between pairs of breeds were statistically significant. The smallest genetic divergence was between Crioulo Lageano and Curraleiro. The National Polled, although historically considered to be of Bos taurus aquitanicus origin, similar to the Caracu, was grouped together with the other breeds of Bos taurus ibericus origin. Generally, the individual breeds formed distinct clusters except the National Polled. The RAPD technique was capable to distinguish genetically between the breeds studied; the Caracu, Crioulo Lageano, Curraleiro and Pantaneiro may be considered distinct genetic entities thereby proving the uniqueness of the populations; the National Polled has not been able to re-establish itself after its decline in the 1950s, thereby losing its genetic identity.
Resumo -Com o intuito de testar a origem da raça Santa Inês, bem como avaliar as unicidades das p... more Resumo -Com o intuito de testar a origem da raça Santa Inês, bem como avaliar as unicidades das populações das mais importantes raças naturalizadas brasileiras de ovinos deslanados foi feito um estudo dos padrões de semelhança molecular obtidos a partir de marcadores RAPD-PCR. Foram utilizados 238 indivíduos provenientes dos Estados de Goiás, Sergipe, Bahia, Distrito Federal, e Ceará distribuídos entre as raças Santa Inês, Bergamácia, Rabo Largo, Morada Nova e Somalis. Ao final da triagem de 140 primers foram selecionados 54 loci a partir de 19 primers. As análises intra-raciais mostraram a presença de marcadores específicos entre as raças analisadas. Nas análises inter-raciais, todas as raças foram significativamente diferentes entre si (Teste Exato de Fisher p<0,01). Paralelamente, foi observado pela análise de variância molecular (AMOVA), que apenas 14,92% da variação total (p<0,01) foi em razão de diferenças inter-raciais. A análise de agrupamento mostrou que a Raça Santa Inês ficou mais próxima da Bergamácia (97% bootstrapping) e que a raça Rabo Largo teria maior similaridade a este grupo do que a raça Morada Nova e Somalis (81% bootstrapping). Os resultados sugerem que medidas para conservação e manejo já podem ser planejadas e que novos estudos abrangendo tanto loci de microssatélites e mitocondriais como a inclusão de novas raças lanadas poderão fornecer uma melhor base para confirmar a origem da raça Santa Inês.
Pesquisa Agropecuaria Brasileira
The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep... more The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep breeds and to determine the origin of the Santa Inês breed. Molecular similarity was determined using Randomly Amplified Polymorphic DNA -Polymerase Chain Reaction markers in 238 individuals from five naturalized sheep breeds: Santa Inês (48 animals), Rabo Largo , Somali (48), Morada Nova and Bergamasca , collected in Goiás, Sergipe, Bahia, and Ceará States as well as in the Federal District. Fifty-four loci were selected from 19 primers, after a pilot test using 140 primers. Qualitative analyses indicate diagnostic markers for all breeds. All breeds were significantly different from each other. Interbreed differences were explained by 14.92% of the total variation. Santa Inês clustered with Bergamasca (97% bootstrap) and with Rabo Largo, composing the third member of the group (81% bootstrap) while Morada Nova and Somali breeds clustered separately. Each breed should be considered as a separate management and conservation unit, and special care should be taken with Rabo Largo, Morada Nova and Somali breeds, represented by small herds in Brazil.
Pesquisa Agropecuária Brasileira
The objective of this study was to verify the genetic diversity between and within seven populati... more The objective of this study was to verify the genetic diversity between and within seven populations of Moxoto goat (n = 264) from the States of Pernambuco, Paraiba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxoto, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, white 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Flor...
investigación y otra almacenada en el Banco de ADN, mantenida en congelador a -80 o C. El Banco c... more investigación y otra almacenada en el Banco de ADN, mantenida en congelador a -80 o C. El Banco cuenta con 11.118 muestras de ADN de vacunos, equinos, mulas, búfalos, cabras y ovejas, así como aves y algunas especies productivas de tortugas, peces y roedores. El Banco contiene material para la caracterización molecular de las razas y la identificación de genes de interés. Su formación se debe al esfuerzo de investigadores y colaboradores, lo que refuerza la importancia de alianzas en la Conservación y Uso de los Recursos Genéticos Animales en Brasil. El futuro del banco será la utilización de herramientas estadísticas para comprobar y ajustar su alcance nacional y guía nuevas recogidas en hatos relacionados con programas de pre-mejora y mejora, además de dirigir la selección de donantes de Banco de Germoplasma.
As the aim of investigating the population structure of Brazilian bovine locally adapted breeds, ... more As the aim of investigating the population structure of Brazilian bovine locally adapted breeds, 237 individuals from Caracu (n = 50), Creole Lageana (n = 39), Curraleira (n = 50), Mocha Nacional (n = 50) and Pantaneira breeds (n = 48) were genotyped with 28 microsatellite loci chosen from lists of FAO / ISAG and BIOBOVIS project. Indices of genetic diversity were calculated by FSAT program and the genetic population structure was obtained based on Bayesian analysis implemented by the STRUCTURE program. There was a significant genetic differentiation (p <0.05) between breeds studied, the percentage of variation among populations was 4.64%. In general the expected heterozygosity was higher than observed heterozygosity (0.751 vs 0.696). The Creole Lageana breed showed the highest allelic richness (8.9) while the lowest was observed in the Caracu breed (7.07). The greatest FIS was observed in Curraleira (0.106) and the smallest in the Mocho National (0.035). By the Bayesian analysis...
Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. 23 p. – (Documentos / Embrapa Rec... more Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. 23 p. – (Documentos / Embrapa Recursos Genéticos e Biotecnologia, ISSN 0102-0110 ; 166)
Theriogenology, 2002
Brazil has various species of domestic animals, which developed from breeds brought by the Portug... more Brazil has various species of domestic animals, which developed from breeds brought by the Portuguese settlers soon after their discovery. For five centuries, these breeds have been subjected to natural selection in specific environments. Today, they present characteristics adapted to the specific Brazilian environmental conditions. These breeds developed in Brazil are known as "Crioulo," "local," or naturalized. From the beginning of the 20th century, some exotic breeds, selected in temperate regions, have begun to be imported. Although more productive, these breeds do not have adaptive traits, such as resistance to disease and parasites found in breeds considered to be "native." Even so, little by little, they replaced the native breeds, to such an extent that the latter are in danger of extinction. In 1983, to avoid the loss of this important genetic material, the National Research Center for Genetic Resources and Biotechnology (Cenargen) of the Brazilian Agricultural Research Corporation (Embrapa) decided to include conservation of animal genetic resources in its research program Conservation and Utilization of Genetic Resources. Until this time, they were only concerned with conservation of native plants. Conservation has been carried out by various research centers of Embrapa, universities, state research corporations, and private farmers, with a single coordinator at the national level, Cenargen. Specifically, conservation is being carried out by conservation nuclei, which are specific herds in which the animals are being conserved, situated in the habitats where the animals have been subjected to natural selection. This involves storage of semen and embryos from cattle, horses, buffaloes, donkeys, goats, sheep, and pigs. The Brazilian Animal Germplasm Bank is kept at Cenargen, which is responsible for the storage of semen and embryos of various breeds of domestic animals threatened with extinction, where almost 45,000 doses of semen and more than 200 embryos exist presently. An important challenge for this program is to make the different segments of society realize the importance of the conservation of animal genetic resources.
Corumbá: Embrapa Pantanal, 2003. 29 p.; 21 cm (Documentos / Embrapa Pantanal ISSN 1517-1973; 55).
O objetivo deste trabalho foi caracterizar uma população de animais da raça Nelore Variedade Verm... more O objetivo deste trabalho foi caracterizar uma população de animais da raça Nelore Variedade Vermelha visando estimar parâmetros populacionais e sua composição genética mediante comparação com raças localmente adaptadas e com a raça Nelore Padrão. Foram genotipados nove locos microssatélites em 192 indivíduos das seguintes raças: Caracu (40), Curraleiro (40), Pantaneiro (40), Nelore Padrão (40) e Vermelho (32). Todas as raças exibiram uma redução significativa na heterozigosidade observada (Ho), quando comparada com a heterozigosidade esperada (He) devido a acasalamentos que não ocorreram ao acaso dentro das populações. A raça Nelore Vermelha apresentou valores mais altos de heterozigosidade esperada e observada (0,756 e 0,707, respectivamente). Os resultados obtidos mostraram que existe variabilidade genética significativa em todos os grupos estudados (> 0,68). A relação entre os grupamentos genéticos foi analisada. As populações Nelore Padrão e Variedade Vermelha (Bos indicus) ficaram agrupadas distintamente das demais raças Bos taurus (Caracu, Curraleiro e Pantaneiro). As estimativas de diferenciação genética aos pares indicam que todas as populações estudadas podem ser consideradas entidades geneticamente independentes (p<0,01), embora não tenha sido possível distinguir, por método de agrupamento Bayesiano, as diferentes linhagens da raça Nelore. Palavras-chave: diversidade genética, genética de populações, STR, Structure
Objetivou-se analisar a frequência gênica de polimorfismos dos genes estearoil-CoA dessaturase (S... more Objetivou-se analisar a frequência gênica de polimorfismos dos genes estearoil-CoA dessaturase (SCD1) e ácido graxo sintase (FASN) em diferentes raças taurinas localmente adaptadas. Os animais foram genotipados para os polimorfismos g.16024 A>G e g.16039 T>C do gene FASN pela técnica de PCR-RFLP e os polimorfismos SCD1-702 G>A e SCD1-762 C>T do gene SCD1 por sequenciamento. As frequências do haplótipo favorável TW do gene FASN foi maior nas raças Crioula Lageana e Caracu em relação às demais. Em relação ao gene SCD1, para o polimorfismo SCD1-702 A>G, as raças Curraleira, Crioula Lageana, e Pantaneira apresentaram menor frequência do alelo favorável A (0,05, 0,01 e 0,11) e para o polimorfismo SCD1-762 C>T, apresentam frequências mais altas do alelo favorável T de 0,39, 0,45 e 0,68, respectivamente. Devido a essas divergências entre as freqüências, sugeriu-se a validação desses SNPs com dados fenotípicos. Houve uma alta variabilidade genética dos polimorfismos avaliados nas raças localmente adaptadas, evidenciando seu potencial de uso em programas de melhoramento genético. Palavras-chave: bovinos, FASN, perfil de ácidos graxos, qualidade de carne, SCD1, SNP