Andreas Kistler - Academia.edu (original) (raw)
Papers by Andreas Kistler
PubMed, 1984
Mixed micelles, with their main constituents lecithin and glycocholic acid, form a new principle ... more Mixed micelles, with their main constituents lecithin and glycocholic acid, form a new principle for the parenteral administration of compounds which are poorly water-soluble. Their composition of mainly physiological substances as well as their comparatively good stability substantiate their attractivity in comparison to existing solvents. A decomposition due to physical influences such as heat or storage for several years will almost exclusively affect the lecithin component in the form of hydrolysis into free fatty acids and lysolecithin. Their toxicity was examined experimentally in various studies using both undecomposed and artificially decomposed mixed micelles. In these studies the mixed micelles were locally and systemically well tolerated and proved to be neither embryotoxic, teratogenic nor mutagenic. Only when comparatively high doses of the undecomposed mixed micelles were administered, corresponding to approximately 30 to 50 times the anticipated clinical injection volume (of e.g. diazepam mixed micelles), did some vomitus (dogs), slight liver enzyme elevation (rats and dogs), and slightly increased liver weights (dogs) occur. After repeated injections of the artificially decomposed formulation (approximately 25% of lecithin hydrolyzed to free fatty acids and lysolecithin) effects such as intravascular haemolysis, liver enzyme elevations and intrahepatic cholestasis (dogs only) were observed but only when doses exceeding a threshold of approximately 40 to 60 mg lysolecithin/kg body weight were administered. All alterations were reversible after cessation of treatment.
Experientia, Apr 1, 1995
Some physicians use the electrical conductance of the skin, particularly at the acupuncture point... more Some physicians use the electrical conductance of the skin, particularly at the acupuncture points, for diagnostic purposes. This paper deals with the quantification of the skin conductance at some acupuncture points under well defined conditions using the electrode materials gold, graphite, silver and brass. The observed current response appeared to be best described by two exponentials.
Birth Defects Research, Apr 1, 1981
Experimental Cell Research, Mar 1, 1975
Xenobiotica, 1985
A new apparatus for the mild and reproducible dissociation of rat limb buds into single cells is ... more A new apparatus for the mild and reproducible dissociation of rat limb buds into single cells is described. The growth and differentiation of the embryonic mesenchyme cells into chrondrocytes was markedly affected by the conditions of tissue dissociation and the serum used. The degree of chondrogenesis was assessed by alcian blue staining, specific for cartilage matrix. Retinoic acid, a known teratogen, inhibited chondrogenesis completely in a dose-dependent manner. The determination of the quantifiable end-point, IC50 in this cell-culture system, may be suitable to compare the activity of various retinoids and eventually to determine their teratogenic potential.
Complementary Medicine Research, 1999
SummaryThis study served to compare the effect of needling at true acupuncture points with that o... more SummaryThis study served to compare the effect of needling at true acupuncture points with that of needling at non-acupuncture points. Various parameters were used to quantify the results such as measurement of the peripheral blood circulation in finger tips and the nasal mucous membrane, measurement of the skin temperature at various sites of the body by means of infrared thermography or contact thermometry, testing of skin conductance, or recording of heart frequency and respiration. These are all indicators of the functional state of the autonomic nervous system. This model experiment was performed on healthy volunteers and showed identical, transient, quick reflex responses of the sympathetic nervous system after true acupuncture as well as after needling at non-acupuncture points. These reactions are related to pain and do not allow to draw any conclusions on the specificity of the points that were needled. The aim of this project was not to make a statement about the therapeutic efficacy of acupuncture. It was meant to be a model allowing the study of some basic regulatory mechanisms which are fundamental for most treatments in complementary medicine.
Complementary Medicine Research, 1997
Summary The goal of the present study was to investigate whether psychoneuroimmunological variabl... more Summary The goal of the present study was to investigate whether psychoneuroimmunological variables including salivary cortisol and immunoglobulin A (S-IgA) levels as well as autonomic parameters were influenced by olfactory stimulation. Fragrance exposure for 10 minutes to lavender or skatole at concentrations of about 7 times their perception thresholds did not affect saliva cortisol or S-IgA levels and total protein or autonomic variables (heart rate, respiration frequency, electrodermal activity and fingertip microcirculation measured by laser Doppler flowmetry, photoplethysmography and temperature). Considering our detailed analyses of these parameters, including the individual time courses of the autonomic variables, salivary cortisol and S-IgA, and taking into account the degree of pleasure and the type of fragrance, it is noteworthy that no effects were observed. We conclude that the stimulation elicited by the tested fragrances was too weak to affect psychoneuroimmunological variables.Copyright © 1997 S. Karger AG, Basel
PubMed, 1994
It has been postulated and there are indications that acupuncture affects the cardiovascular syst... more It has been postulated and there are indications that acupuncture affects the cardiovascular system via the autonomic nervous system. The blood vessels of the hand skin have sympathetic constrictor nerves only. Therefore, the possible effect of acupuncture on the ANS can be investigated by measurements of microvascular perfusion and thermoregulation of the hands. Knowing the physiological effects of acupuncture may help to improve acceptance of this unconventional medical approach.
Complementary Medicine Research, 1998
Summary Saliva collection with salivettes ® for determination of salivary cortisol and other comp... more Summary Saliva collection with salivettes ® for determination of salivary cortisol and other components is a commonly used technique that is quick and hygienic. The goal of the present study was to determine whether chewing of the cotton swab of salivettes® is a sympathetic stimulus and, if yes, whether this stimulus is strong enough to affect salivary cortisol. We found
Complementary Medicine Research, 1999
The goal of the present study was to investigate whether suggestions for cold or warmth during hy... more The goal of the present study was to investigate whether suggestions for cold or warmth during hypnosis affect fingertip skin temperature. Hypnosis without specific suggestions for cold or warmth ('neutral hypnosis') caused a drop in respiration frequency, however, pulse rate, fingertip skin temperature, and electrodermal activity were not affected. The cold and warmth suggestions decreased and increased fingertip skin temperature, respectively. Compared with the neutral trance phase, the other three autonomic variables measured were also affected by suggestions for cold. However, there was no association between the changes in autonomic variables induced by suggestions and hypnotizability scores measured by the 'Stanford Hypnotic Clinical Scale for Adults'. Fingertip skin temperature was mostly affected when the images used for the cold and warmth suggestions during hypnosis included experiences of physical temperature and psychological stress or relaxation, indicating that the psychological content of the imagery amplified the autonomic response.
PubMed, Jun 1, 1996
A probe holder for long-term measurements of intranasal microcirculation by laser Doppler flowmet... more A probe holder for long-term measurements of intranasal microcirculation by laser Doppler flowmetry is described. It is adjustable to any physiognomy and allows precise intranasal probe insertion. It is based on a commercially-available shooting-spectacles frame and might also be useful for other measurements such as intranasal temperature, humidity and pO2.
Complementary Medicine Research, 1996
... needling but no differences between them. Because both acupuncture and placeboneedling caused... more ... needling but no differences between them. Because both acupuncture and placeboneedling caused pain, we investigated whether the observed responses werepain-related. Painless low-level laser stimulation, a treatment ...
International Journal of Biochemistry, 1981
ABSTRACT
In vitro cellular & developmental biology, 1991
Wilhelm Roux's archives of developmental biology, 1985
In cultured fetal rat bones retinoic acid induces the release of proteoglycan, followed by cartil... more In cultured fetal rat bones retinoic acid induces the release of proteoglycan, followed by cartilage tissue breakdown. This correlates with a shortening of the bone of up to 40%. The calmodulin antagonists trifluoperazine, chlorpromazine, diazepam and the naphthalenesulphonamide W-7 inhibited the retinoic acid-induced bone shortening. Inhibition by trifluoperazine and W-7 was noted at 40 and 75 µM, respectively, concentrations which
General and Comparative Endocrinology, Aug 1, 1976
Birth Defects Research, 1986
International Journal of Biochemistry, 1980
Abstract 1. 1. The changes in the free pool, in the uptake and incorporation of leucine and p -fl... more Abstract 1. 1. The changes in the free pool, in the uptake and incorporation of leucine and p -fluorophenylalanine and their relationship to the rate of protein synthesis have been studied in Rana catesbeiana tadpole liver during spontaneous metamorphosis. 2. 2. The amino acid concentrations in the liver during metamorphosis showed a transient increase of all detectable amino acids at stage XXIV. 3. 3. The total amino acid concentration was between 4.7 and 9.8 μmol per g liver and glutamic acid showed the highest concentration at all stages (20–41% of the total amino acids). The leucine content was between 2.0 and 4.9% with lower relative amounts at prometamorphic stages. 4. 4. The uptake into the free pool of leucine and p -fluorophenylalanine decreased during spontaneous metamorphosis. A similar decrease was observed with the model amino acid cycloleucine, whereas α-aminoisobutyric acid uptake at stage XXII was 56% above and, at stage XXV 31% below the stage XII value. 5. 5. The incorporation of leucine and p -fluorophenylalanine into liver protein decreased during spontaneous metamorphosis. 6. 6. However, corrected for the changes in specific activity of leucine the rate of protein synthesis was increased from 20 to 75% between stages XVIII and XXIV but was depressed by 41% at stage XXV compared with stage X to XII.
PubMed, 1984
Mixed micelles, with their main constituents lecithin and glycocholic acid, form a new principle ... more Mixed micelles, with their main constituents lecithin and glycocholic acid, form a new principle for the parenteral administration of compounds which are poorly water-soluble. Their composition of mainly physiological substances as well as their comparatively good stability substantiate their attractivity in comparison to existing solvents. A decomposition due to physical influences such as heat or storage for several years will almost exclusively affect the lecithin component in the form of hydrolysis into free fatty acids and lysolecithin. Their toxicity was examined experimentally in various studies using both undecomposed and artificially decomposed mixed micelles. In these studies the mixed micelles were locally and systemically well tolerated and proved to be neither embryotoxic, teratogenic nor mutagenic. Only when comparatively high doses of the undecomposed mixed micelles were administered, corresponding to approximately 30 to 50 times the anticipated clinical injection volume (of e.g. diazepam mixed micelles), did some vomitus (dogs), slight liver enzyme elevation (rats and dogs), and slightly increased liver weights (dogs) occur. After repeated injections of the artificially decomposed formulation (approximately 25% of lecithin hydrolyzed to free fatty acids and lysolecithin) effects such as intravascular haemolysis, liver enzyme elevations and intrahepatic cholestasis (dogs only) were observed but only when doses exceeding a threshold of approximately 40 to 60 mg lysolecithin/kg body weight were administered. All alterations were reversible after cessation of treatment.
Experientia, Apr 1, 1995
Some physicians use the electrical conductance of the skin, particularly at the acupuncture point... more Some physicians use the electrical conductance of the skin, particularly at the acupuncture points, for diagnostic purposes. This paper deals with the quantification of the skin conductance at some acupuncture points under well defined conditions using the electrode materials gold, graphite, silver and brass. The observed current response appeared to be best described by two exponentials.
Birth Defects Research, Apr 1, 1981
Experimental Cell Research, Mar 1, 1975
Xenobiotica, 1985
A new apparatus for the mild and reproducible dissociation of rat limb buds into single cells is ... more A new apparatus for the mild and reproducible dissociation of rat limb buds into single cells is described. The growth and differentiation of the embryonic mesenchyme cells into chrondrocytes was markedly affected by the conditions of tissue dissociation and the serum used. The degree of chondrogenesis was assessed by alcian blue staining, specific for cartilage matrix. Retinoic acid, a known teratogen, inhibited chondrogenesis completely in a dose-dependent manner. The determination of the quantifiable end-point, IC50 in this cell-culture system, may be suitable to compare the activity of various retinoids and eventually to determine their teratogenic potential.
Complementary Medicine Research, 1999
SummaryThis study served to compare the effect of needling at true acupuncture points with that o... more SummaryThis study served to compare the effect of needling at true acupuncture points with that of needling at non-acupuncture points. Various parameters were used to quantify the results such as measurement of the peripheral blood circulation in finger tips and the nasal mucous membrane, measurement of the skin temperature at various sites of the body by means of infrared thermography or contact thermometry, testing of skin conductance, or recording of heart frequency and respiration. These are all indicators of the functional state of the autonomic nervous system. This model experiment was performed on healthy volunteers and showed identical, transient, quick reflex responses of the sympathetic nervous system after true acupuncture as well as after needling at non-acupuncture points. These reactions are related to pain and do not allow to draw any conclusions on the specificity of the points that were needled. The aim of this project was not to make a statement about the therapeutic efficacy of acupuncture. It was meant to be a model allowing the study of some basic regulatory mechanisms which are fundamental for most treatments in complementary medicine.
Complementary Medicine Research, 1997
Summary The goal of the present study was to investigate whether psychoneuroimmunological variabl... more Summary The goal of the present study was to investigate whether psychoneuroimmunological variables including salivary cortisol and immunoglobulin A (S-IgA) levels as well as autonomic parameters were influenced by olfactory stimulation. Fragrance exposure for 10 minutes to lavender or skatole at concentrations of about 7 times their perception thresholds did not affect saliva cortisol or S-IgA levels and total protein or autonomic variables (heart rate, respiration frequency, electrodermal activity and fingertip microcirculation measured by laser Doppler flowmetry, photoplethysmography and temperature). Considering our detailed analyses of these parameters, including the individual time courses of the autonomic variables, salivary cortisol and S-IgA, and taking into account the degree of pleasure and the type of fragrance, it is noteworthy that no effects were observed. We conclude that the stimulation elicited by the tested fragrances was too weak to affect psychoneuroimmunological variables.Copyright © 1997 S. Karger AG, Basel
PubMed, 1994
It has been postulated and there are indications that acupuncture affects the cardiovascular syst... more It has been postulated and there are indications that acupuncture affects the cardiovascular system via the autonomic nervous system. The blood vessels of the hand skin have sympathetic constrictor nerves only. Therefore, the possible effect of acupuncture on the ANS can be investigated by measurements of microvascular perfusion and thermoregulation of the hands. Knowing the physiological effects of acupuncture may help to improve acceptance of this unconventional medical approach.
Complementary Medicine Research, 1998
Summary Saliva collection with salivettes ® for determination of salivary cortisol and other comp... more Summary Saliva collection with salivettes ® for determination of salivary cortisol and other components is a commonly used technique that is quick and hygienic. The goal of the present study was to determine whether chewing of the cotton swab of salivettes® is a sympathetic stimulus and, if yes, whether this stimulus is strong enough to affect salivary cortisol. We found
Complementary Medicine Research, 1999
The goal of the present study was to investigate whether suggestions for cold or warmth during hy... more The goal of the present study was to investigate whether suggestions for cold or warmth during hypnosis affect fingertip skin temperature. Hypnosis without specific suggestions for cold or warmth ('neutral hypnosis') caused a drop in respiration frequency, however, pulse rate, fingertip skin temperature, and electrodermal activity were not affected. The cold and warmth suggestions decreased and increased fingertip skin temperature, respectively. Compared with the neutral trance phase, the other three autonomic variables measured were also affected by suggestions for cold. However, there was no association between the changes in autonomic variables induced by suggestions and hypnotizability scores measured by the 'Stanford Hypnotic Clinical Scale for Adults'. Fingertip skin temperature was mostly affected when the images used for the cold and warmth suggestions during hypnosis included experiences of physical temperature and psychological stress or relaxation, indicating that the psychological content of the imagery amplified the autonomic response.
PubMed, Jun 1, 1996
A probe holder for long-term measurements of intranasal microcirculation by laser Doppler flowmet... more A probe holder for long-term measurements of intranasal microcirculation by laser Doppler flowmetry is described. It is adjustable to any physiognomy and allows precise intranasal probe insertion. It is based on a commercially-available shooting-spectacles frame and might also be useful for other measurements such as intranasal temperature, humidity and pO2.
Complementary Medicine Research, 1996
... needling but no differences between them. Because both acupuncture and placeboneedling caused... more ... needling but no differences between them. Because both acupuncture and placeboneedling caused pain, we investigated whether the observed responses werepain-related. Painless low-level laser stimulation, a treatment ...
International Journal of Biochemistry, 1981
ABSTRACT
In vitro cellular & developmental biology, 1991
Wilhelm Roux's archives of developmental biology, 1985
In cultured fetal rat bones retinoic acid induces the release of proteoglycan, followed by cartil... more In cultured fetal rat bones retinoic acid induces the release of proteoglycan, followed by cartilage tissue breakdown. This correlates with a shortening of the bone of up to 40%. The calmodulin antagonists trifluoperazine, chlorpromazine, diazepam and the naphthalenesulphonamide W-7 inhibited the retinoic acid-induced bone shortening. Inhibition by trifluoperazine and W-7 was noted at 40 and 75 µM, respectively, concentrations which
General and Comparative Endocrinology, Aug 1, 1976
Birth Defects Research, 1986
International Journal of Biochemistry, 1980
Abstract 1. 1. The changes in the free pool, in the uptake and incorporation of leucine and p -fl... more Abstract 1. 1. The changes in the free pool, in the uptake and incorporation of leucine and p -fluorophenylalanine and their relationship to the rate of protein synthesis have been studied in Rana catesbeiana tadpole liver during spontaneous metamorphosis. 2. 2. The amino acid concentrations in the liver during metamorphosis showed a transient increase of all detectable amino acids at stage XXIV. 3. 3. The total amino acid concentration was between 4.7 and 9.8 μmol per g liver and glutamic acid showed the highest concentration at all stages (20–41% of the total amino acids). The leucine content was between 2.0 and 4.9% with lower relative amounts at prometamorphic stages. 4. 4. The uptake into the free pool of leucine and p -fluorophenylalanine decreased during spontaneous metamorphosis. A similar decrease was observed with the model amino acid cycloleucine, whereas α-aminoisobutyric acid uptake at stage XXII was 56% above and, at stage XXV 31% below the stage XII value. 5. 5. The incorporation of leucine and p -fluorophenylalanine into liver protein decreased during spontaneous metamorphosis. 6. 6. However, corrected for the changes in specific activity of leucine the rate of protein synthesis was increased from 20 to 75% between stages XVIII and XXIV but was depressed by 41% at stage XXV compared with stage X to XII.