Andres Daniel Zambelli - Academia.edu (original) (raw)

Papers by Andres Daniel Zambelli

Journal of Basic and Applied Genetics, 2019

Even when conventional breeding was effective in achieving a continuous improvement in yield, Mol... more Even when conventional breeding was effective in achieving a continuous improvement in yield, Molecular Genetics tools applied in plant breeding contributed to maximize genetic gain. Thus, the use of DNA technology applied in agronomic improvement gave rise to Molecular Breeding, discipline which groups the different breeding strategies where genotypic selection, based on DNA markers, are used in combination with or in replacement of phenotypic selection. These strategies can be listed as: marker-assisted selection; marker-assisted backcrossing; marker assisted recurrent selection; and genomic selection. Strong arguments have been made about the potential advantages that Molecular Breeding brings, although little has been devoted to discussing its feasibility in practical applications. The consequence of the lack of a deep analysis when implementing a strategy of Molecular Breeding is its failure, leading to many undesirable outcomes and discouraging breeders from using the technolo...

Journal of the science of food and agriculture, 2018

The effect of genetic background on the stability of fatty acid composition in sunflower near iso... more The effect of genetic background on the stability of fatty acid composition in sunflower near isogenic lines (NILs) carrying high oleic Pervenets (P) or high oleic NM1 mutations was studied. The materials were field-tested in different locations and sowing dates to evaluate a wide range of environmental conditions. Relationships between the fatty acids and the minimum night temperature (MNT) were established and the response was characterized. Genetic background effect for the fatty acid composition was found in both groups of NILs. NM1-NILs showed an oleic level higher than 910 g kg-1 and they were more stable across environments with a null or low dependence from the genetic background; contrarily, high oleic materials bearing the P mutation showed lower levels of oleic acid, with a higher variation in fatty acid composition and a highly significant dependence with the genetic background. NM1 mutation is the best option to develop ultra-high oleic sunflower oil stable across envir...

Journal of the American Oil Chemists' Society

Genetica, Jun 4, 2010

South American camelids comprise the guanaco (Lama guanicoe) and the vicuña (Vicugna vicugna), wh... more South American camelids comprise the guanaco (Lama guanicoe) and the vicuña (Vicugna vicugna), which are wild species, and the domestic llama (Lama glama) and alpaca (Lama pacos). This paper presents the first complete mitochondrial (mt) genome of the guanaco and the mt coding sequence of the vicuña. The guanaco mtDNA is 16,649 nt long and its composition and organization are similar to the mitochondrial genome of other mammals. Excluding the control region, comparison of the complete guanaco and vicuña mtDNA showed 4.4% sequence divergence. Nucleotide differences in peptide coding genes varied from 1.9% in ATP6 to 6.4% in Cyt b. These values are compatible with the close relatedness of both species identified by other authors. Based on the differences between the control region sequence here reported and that previously described, we also discuss the occurrence of NUMTs in the genome of South American camelids.

J Bioenerg Biomembrane, 2009

ATP synthase is an enzyme involved in oxidative phosphorylation from prokaryotic to eukaryotic ce... more ATP synthase is an enzyme involved in oxidative phosphorylation from prokaryotic to eukaryotic cells. In mammals it comprises at least 16 subunits from which the mitochondrial encoded ATP6 and ATP8 are essential. Mitochondrial genes variations have been suggested to allow rapid human and animal adaptation to new climates and dietary conditions (Mishmar et al. 2003). Camelidae taxa are uniquely adapted to extremely hot and dry climates of African-Asian territories and to cold and hypoxic environments of the South American Andean region. We sequenced and analyzed ATP6 and ATP8 genes in all camelid species. Based on the available structural data and evolutionary conservation of the deduced proteins we identified features proper of the group. In Old World camels the ATP8, important in the assembly of the F0 complex, showed a number of positively charged residues higher than in the other aligned species. In ATP6 we found the camelid specific substitutions Q47H and I106V that occur in sites highly conserved in other species. We speculate that these changes may have functional importance.

ABSTRACT Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide res... more ABSTRACT Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide resistant and wild type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

ABSTRACT Isolated nucleotide sequences mutated by insertion encoding a truncated sunflower oleate... more ABSTRACT Isolated nucleotide sequences mutated by insertion encoding a truncated sunflower oleate desaturase protein, truncated protein, methods, procedures and uses. The isolated nucleotide sequences comprise an insertion that includes a stop codon, and wherein the sequences encode a truncated sunflower oleate desaturase protein. The truncated sunflower oleate desaturase protein may be for example the sequence shown in SEQ ID No: 1 or SEQ ID No: 2.

ABSTRACT A sorghum seed comprising in its genome at least one polynucleotide encoding a polypepti... more ABSTRACT A sorghum seed comprising in its genome at least one polynucleotide encoding a polypeptide having an alanine to threonine substitution at position 93 of the sorghum AHAS protein large subunit. The plant has increased resistance to one or more herbicides, for example from the imidazolinone group, as compared to wild-type sorghum plants. The sorghum plant may comprise in its genome, one, two, three or more copies of a polynucleotide encoding a mutated large subunit of sorghum AHAS or a sorghum AHAS polypeptide of the invention. In this context, the sorghum plant may be tolerant to any herbicide capable of inhibiting AHAS enzyme activity. For example, the sorghum plant may be tolerant to herbicides of the imidazolinones type, such as imazethapyr, imazapir, and imazapic or to herbicides of the sulfonylurea group.

Journal of the Science of Food and Agriculture, 2016

Temperature modified the triacylglycerol species in high stearic sunflower lines.-Triacylglycerol... more Temperature modified the triacylglycerol species in high stearic sunflower lines.-Triacylglycerol symmetry was higher in lines with high linoleic than high oleic background.-Increasing temperature produced a more symmetrical distribution of saturated fatty acids.-This effect was additional to the effect of temperature on fatty acid composition.

ABSTRACT Novel herbicide-resistant sunflower plants designated as MUT31 and herbicide-resistant d... more ABSTRACT Novel herbicide-resistant sunflower plants designated as MUT31 and herbicide-resistant descendents thereof are provided. The MUT31 sunflower plants and the herbicide-resistant descendents thereof comprise increased resistance to at least one imidazolinone herbicide, when compared to wild-type sunflower plants. Methods for controlling weeds in the vicinity of these herbicide-resistant sunflower plants and methods for increasing the herbicide-resistance of a sunflower plant are also provided.

Comprehensive Reviews in Food Science and Food Safety, 2008

The invention relates to isolated nucleotide sequences, comprising a point mutation and wherein t... more The invention relates to isolated nucleotide sequences, comprising a point mutation and wherein the sequences encode a modified oleate desaturase sunflower protein. The modified oleate desaturase sunflower protein comprises at least one of the following amino acid substitutions: a) Gly by Asp at position 103, b) Ser by Leu at position 131, c) Ser by Asn at position 135, d) Gly by Glu at position 144,e) Gly by Glu at position 226, f) Pro by Ser at position 253, g) Leu by Phe at position 267, h) Pro by Ser at position 275, or it is a truncated oleate desaturase.

Caryologia, 1988

Content should not be relied upon and should be independently verified with primary sources of in... more Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden.

Mammalian Biology - Zeitschrift fur Saugetierkunde

Studied bone-marrow banded and Ag-NORs karyotypes of the South American phyllotine Eligmodontia i... more Studied bone-marrow banded and Ag-NORs karyotypes of the South American phyllotine Eligmodontia in three populations of northern Patagonia (Argentina): south of Nahuel Huapi Lake and Los Menucos (Rio Negro Province), and Junin de los Andes (Neuquén Province). In the first locality, two polymorphic variants (2n=34 and 2n=32) were found; in the other two localities the 2n=44 karyomorph previously reported as belonging to Eligmodontia typus, was dominant, but it was found in sympatry with two polymorphic variants (2n=33-34) corresponding to the Nahuel Huapi karyotype, thus confirming that these karyomorphs belong to different, probably synmorphic species

Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide resistant an... more Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide resistant and wild type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

The present invention relates to a method for increasing the SUS content in an oil or in an olein... more The present invention relates to a method for increasing the SUS content in an oil or in an olein fraction, comprising performing 1, 3-selective enzymatic intraesterification on a natural starting oil or olein fraction prepared therefrom wherein the ratio between SUS and SUU is at least 1:1.5 and the SSS content is low, in particular close to 0%. Further, the present invention relates to 1, 3-Selective intraesterified oil or olein, obtainable by performing the present method.

Novel herbicide-resistant sunflower plants designated as MUT31 and herbicide-resistant descendent... more Novel herbicide-resistant sunflower plants designated as MUT31 and herbicide-resistant descendents thereof are provided. The MUT31 sunflower plants and the herbicide-resistant descendents thereof comprise increased resistance to at least one imidazolinone herbicide, when compared to wild-type sunflower plants. Methods for controlling weeds in the vicinity of these herbicide-resistant sunflower plants and methods for increasing the herbicide-resistance of a sunflower plant are also provided.