Andres Romero - Academia.edu (original) (raw)
Papers by Andres Romero
Vaccine, 2007
Twenty-nine classical swine fever virus (CSFv) strains were grown in the PK15 or SK6 cell lines. ... more Twenty-nine classical swine fever virus (CSFv) strains were grown in the PK15 or SK6 cell lines. Antigenic differentiation studies were performed using monoclonal antibodies (McAbs), produced at Lelystad (CDI-DLO), The Netherlands. The monoclonals which were classified numerically as monoclonals 2-13. Epitope map patterns that resulted from the reactivity with McAbs were found to be unrelated to the pathogenicity of the viruses studied. Antigenic determinants were recognized by McAbs 5 and 8, were not detected in some Mexican strains; however, sites for McAb 6 were absent in all strains. The PAV-250 vaccine strain was recognized by all MAbs, except by MAb 6. Furthermore, the Chinese C-S vaccine strain was found to be very similar to the GPE − vaccine. None of the studied Mexican vaccines or field strains was found to be similar to the PAV-250 vaccine strain.
Journal of Biomedical Materials Research, 2000
Cellular channels during development and after peripheral nerve injury are thought to provide gui... more Cellular channels during development and after peripheral nerve injury are thought to provide guidance cues to growing axons. In tissue culture where these cues are absent, neurites from dorsal root ganglion neurons grow with a radial distribution. To induce directional axonal growth and to enhance the rate of axonal growth after injury, we have designed microfilaments of poly(L-lactide). We demonstrate that dorsal root ganglia grown on these filaments in vitro extend longitudinally oriented neurites in a manner similar to native peripheral nerves. The extent of neurite growth was significantly higher on laminin-coated filaments compared with uncoated and poly-L-lysine-coated filaments. As high as 5.8 +/- 0.2 mm growth was observed on laminin-coated filaments compared with 2.0 +/- 0.2 mm on uncoated and 2.2 +/- 0.3 mm on poly-L-lysine-coated filaments within 8 days. Schwann cells were found to grow on all types of filaments. They were, however, absent in the leading edges of growth on laminin-coated filaments. Photolysis of Schwann cells caused a significant reduction in the neurite length on all types of filaments. Laminin-coated filaments, however, induced significantly longer neurites compared with uncoated and/or poly-L-lysine-coated filaments even in the absence of Schwann cells. Our results suggest that laminin-coated poly(L-lactide) filaments are suitable for inducing directional and enhanced axonal growth. Implants designed by arranging these microfilaments into bundles should aid regenerating axons by providing guidance cues and channels to organize matrix deposition, cell migration, axon growth, and improve functional recovery.
Tissue Engineering, 2003
A simple and repeatable method is described for wet-spinning poly(L-lactic acid) (PLLA) and poly(... more A simple and repeatable method is described for wet-spinning poly(L-lactic acid) (PLLA) and poly(DL-lactic-co-glycolic acid) (PLGA) monofilament fibers. These fibers are strong, elastic, and suitable for many applications, including use as tissue-engineering scaffolds. The PLLA wet-extruded fibers do not show additional strain-induced crystallization as a result of drawing the fibers during fabrication; however, there is an apparent increase in crystallinity late in the degradation process in saline at 37°C. We have measured the molecular weight degradation in saline at 37°C for fibers of both PLLA and PLGA. Changing solvent systems, polymer blends, and winding rates alters mechanical and morphological properties of these fibers for specific applications. The authors discuss a possible theoretical explanation for these observed changes due to changes in polymer concentration, solvent system, and coagulation bath properties. This wet-extrusion process is simple and inexpensive enough to be carried out in almost any laboratory interested in tissue engineering.
Ground Water, 2009
Hydraulic conductivity values computed using the steady-state discharge and drawdown attained whi... more Hydraulic conductivity values computed using the steady-state discharge and drawdown attained while low-flow sampling were evaluated to determine if they were equivalent to those determined from slug testing. Based on testing 12 wells, it was found that the results were statistically equivalent. Conductivity values computed using low-flow sampling parameters were also evaluated as to their reproducibility in actual practice by analyzing consultant data for three wells sampled over three quarterly monitoring periods by four field technicians. The results were found to be reproducible within about a factor of 2 or better. Since the method is based on only one pair of parameters, diligence is required in attaining steady state and in accurately measuring the flow rate and drawdown. Conductivity values computed using this approach can enhance the use of low-flow data gathered in water quality sampling, avoid the need for slug testing in a subsequent phase of investigation, and help reduce the cost of characterizing sites when multilevel samplers are used. Given the practical range of discharge in low-flow sampling, the method was found to be applicable at conductivity values somewhat greater than 10−6 cm/s. Given the typical accuracy of water level meters and pressure transducers and a maximum discharge of 1 L/min, as mandated by regulatory guidance, the method has a calculated upper conductivity limit in the range of 10−3 to 10−2 cm/s.
Vaccine, 2007
Twenty-nine classical swine fever virus (CSFv) strains were grown in the PK15 or SK6 cell lines. ... more Twenty-nine classical swine fever virus (CSFv) strains were grown in the PK15 or SK6 cell lines. Antigenic differentiation studies were performed using monoclonal antibodies (McAbs), produced at Lelystad (CDI-DLO), The Netherlands. The monoclonals which were classified numerically as monoclonals 2-13. Epitope map patterns that resulted from the reactivity with McAbs were found to be unrelated to the pathogenicity of the viruses studied. Antigenic determinants were recognized by McAbs 5 and 8, were not detected in some Mexican strains; however, sites for McAb 6 were absent in all strains. The PAV-250 vaccine strain was recognized by all MAbs, except by MAb 6. Furthermore, the Chinese C-S vaccine strain was found to be very similar to the GPE − vaccine. None of the studied Mexican vaccines or field strains was found to be similar to the PAV-250 vaccine strain.
Journal of Biomedical Materials Research, 2000
Cellular channels during development and after peripheral nerve injury are thought to provide gui... more Cellular channels during development and after peripheral nerve injury are thought to provide guidance cues to growing axons. In tissue culture where these cues are absent, neurites from dorsal root ganglion neurons grow with a radial distribution. To induce directional axonal growth and to enhance the rate of axonal growth after injury, we have designed microfilaments of poly(L-lactide). We demonstrate that dorsal root ganglia grown on these filaments in vitro extend longitudinally oriented neurites in a manner similar to native peripheral nerves. The extent of neurite growth was significantly higher on laminin-coated filaments compared with uncoated and poly-L-lysine-coated filaments. As high as 5.8 +/- 0.2 mm growth was observed on laminin-coated filaments compared with 2.0 +/- 0.2 mm on uncoated and 2.2 +/- 0.3 mm on poly-L-lysine-coated filaments within 8 days. Schwann cells were found to grow on all types of filaments. They were, however, absent in the leading edges of growth on laminin-coated filaments. Photolysis of Schwann cells caused a significant reduction in the neurite length on all types of filaments. Laminin-coated filaments, however, induced significantly longer neurites compared with uncoated and/or poly-L-lysine-coated filaments even in the absence of Schwann cells. Our results suggest that laminin-coated poly(L-lactide) filaments are suitable for inducing directional and enhanced axonal growth. Implants designed by arranging these microfilaments into bundles should aid regenerating axons by providing guidance cues and channels to organize matrix deposition, cell migration, axon growth, and improve functional recovery.
Tissue Engineering, 2003
A simple and repeatable method is described for wet-spinning poly(L-lactic acid) (PLLA) and poly(... more A simple and repeatable method is described for wet-spinning poly(L-lactic acid) (PLLA) and poly(DL-lactic-co-glycolic acid) (PLGA) monofilament fibers. These fibers are strong, elastic, and suitable for many applications, including use as tissue-engineering scaffolds. The PLLA wet-extruded fibers do not show additional strain-induced crystallization as a result of drawing the fibers during fabrication; however, there is an apparent increase in crystallinity late in the degradation process in saline at 37°C. We have measured the molecular weight degradation in saline at 37°C for fibers of both PLLA and PLGA. Changing solvent systems, polymer blends, and winding rates alters mechanical and morphological properties of these fibers for specific applications. The authors discuss a possible theoretical explanation for these observed changes due to changes in polymer concentration, solvent system, and coagulation bath properties. This wet-extrusion process is simple and inexpensive enough to be carried out in almost any laboratory interested in tissue engineering.
Ground Water, 2009
Hydraulic conductivity values computed using the steady-state discharge and drawdown attained whi... more Hydraulic conductivity values computed using the steady-state discharge and drawdown attained while low-flow sampling were evaluated to determine if they were equivalent to those determined from slug testing. Based on testing 12 wells, it was found that the results were statistically equivalent. Conductivity values computed using low-flow sampling parameters were also evaluated as to their reproducibility in actual practice by analyzing consultant data for three wells sampled over three quarterly monitoring periods by four field technicians. The results were found to be reproducible within about a factor of 2 or better. Since the method is based on only one pair of parameters, diligence is required in attaining steady state and in accurately measuring the flow rate and drawdown. Conductivity values computed using this approach can enhance the use of low-flow data gathered in water quality sampling, avoid the need for slug testing in a subsequent phase of investigation, and help reduce the cost of characterizing sites when multilevel samplers are used. Given the practical range of discharge in low-flow sampling, the method was found to be applicable at conductivity values somewhat greater than 10−6 cm/s. Given the typical accuracy of water level meters and pressure transducers and a maximum discharge of 1 L/min, as mandated by regulatory guidance, the method has a calculated upper conductivity limit in the range of 10−3 to 10−2 cm/s.