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Papers by Andrzej B Krajewski

Research paper thumbnail of Remarks Concerning the Nomenclature of Electrical Injuries

Polish Journal of Surgery, 2010

Research paper thumbnail of History of Necrotic Burn Wound Debridement

Polish Journal of Surgery, 2010

Research paper thumbnail of Rekonstrukcja ubytku tkanek miękkich płatem z mięśnia najszerszego grzbietu po oparzeniu głębokim obręczy barkowej z odsłonięciem struktur kostnych u chorego niedożywionego z upośledzeniem odporności – opis przypadku

Chirurgia Plastyczna i Oparzenia / Plastic Surgery and Burns, 2014

Research paper thumbnail of Zespół cieśni śródbrzusznej jako powikłanie w leczeniu wstrząsu oparzeniowego – definicja, zapobieganie, leczenie

Chirurgia Plastyczna i Oparzenia / Plastic Surgery and Burns, 2014

Research paper thumbnail of Active Dynamic Infrared Thermal Imaging in Burn Depth Evaluation

Journal of Burn Care & Research, 2014

The aim of this study was to find the relationship between active dynamic thermography (ADT) with... more The aim of this study was to find the relationship between active dynamic thermography (ADT) with cold excitation and burn depth. This new modality of evaluation of burns seems to be an attractive proposal for quantitative classification, allowing proper choice of burn wound treatment: conservative or surgical, especially compared with static thermography. The work was an in vivo experiment on domestic pigs, and a small number of patients were also diagnosed as part of the study. Statistical analysis showed a high correlation between the ADT synthetic parameter--thermal time constant, τ--and the classification of burn wounds that were predicted to heal within 3 weeks and so were treated conservatively and those that were predicted to not heal within 3 weeks and so were surgically treated. The results of the study show an accuracy of 60.7% for clinical evaluation, 69.6% for static thermography, 83.0% for ADT, and 84.0% for histopathologic assessment. The authors have concluded that the ADT method with cold excitation is suitable for the qualitative and quantitative assessment of burn depth.

Research paper thumbnail of Stem Cells, Including a Population of Very Small Embryonic-Like Stem Cells, are Mobilized Into Peripheral Blood in Patients After Skin Burn Injury

Stem Cell Reviews and Reports, 2011

Background Developmentally early cells, including hematopoietic stem progenitor cells (HSPCs), as... more Background Developmentally early cells, including hematopoietic stem progenitor cells (HSPCs), as well as very small embryonic-like stem cells (VSELs), are mobilized into peripheral blood (PB) in response to tissue and organ injury (e.g., heart infarct or stroke). Objective We seek to determine whether these cells are also mobilized into PB in patients with skin burn injuries. Methods Forty-four (44) patients (33-57 years of age) with total body surface burn area of 30-60%, as well as 23 healthy control subjects, were recruited and PB samples were harvested during the first 24 hours, day +2, and day +5 after burn injury and compared to normal controls. The circulating human CD34 + CD133 + cells enriched for HSPCs, as well as small CXCR4 + CD34 + CD133 + subsets of Lin-CD45cells that correspond to the population of VSELs, were counted by FACS and evaluated by direct immunofluorescence staining for pluripotency markers (Oct-4, Nanog, and SSEA-4). In parallel, we also measured by ELISA the serum concentration of factors that regulate stem cell trafficking, such as SDF-1, VEGF, and HGF. Results Our data indicate that skin burn injury mobilizes cells expressing stem cell-associated markers, such as CD133, CD34, and CXCR4, into PB. More importantly, we found an increase in the number of circulating primitive, small Oct-4 + Nanog + SSEA-4 + CXCR4 + lin-CD45-VSELs. All these changes were accompanied by increased serum concentrations of SDF-1 and VEGF.

Research paper thumbnail of Remarks Concerning the Nomenclature of Electrical Injuries

Polish Journal of Surgery, 2010

Research paper thumbnail of History of Necrotic Burn Wound Debridement

Polish Journal of Surgery, 2010

Research paper thumbnail of Rekonstrukcja ubytku tkanek miękkich płatem z mięśnia najszerszego grzbietu po oparzeniu głębokim obręczy barkowej z odsłonięciem struktur kostnych u chorego niedożywionego z upośledzeniem odporności – opis przypadku

Chirurgia Plastyczna i Oparzenia / Plastic Surgery and Burns, 2014

Research paper thumbnail of Zespół cieśni śródbrzusznej jako powikłanie w leczeniu wstrząsu oparzeniowego – definicja, zapobieganie, leczenie

Chirurgia Plastyczna i Oparzenia / Plastic Surgery and Burns, 2014

Research paper thumbnail of Active Dynamic Infrared Thermal Imaging in Burn Depth Evaluation

Journal of Burn Care & Research, 2014

The aim of this study was to find the relationship between active dynamic thermography (ADT) with... more The aim of this study was to find the relationship between active dynamic thermography (ADT) with cold excitation and burn depth. This new modality of evaluation of burns seems to be an attractive proposal for quantitative classification, allowing proper choice of burn wound treatment: conservative or surgical, especially compared with static thermography. The work was an in vivo experiment on domestic pigs, and a small number of patients were also diagnosed as part of the study. Statistical analysis showed a high correlation between the ADT synthetic parameter--thermal time constant, τ--and the classification of burn wounds that were predicted to heal within 3 weeks and so were treated conservatively and those that were predicted to not heal within 3 weeks and so were surgically treated. The results of the study show an accuracy of 60.7% for clinical evaluation, 69.6% for static thermography, 83.0% for ADT, and 84.0% for histopathologic assessment. The authors have concluded that the ADT method with cold excitation is suitable for the qualitative and quantitative assessment of burn depth.

Research paper thumbnail of Stem Cells, Including a Population of Very Small Embryonic-Like Stem Cells, are Mobilized Into Peripheral Blood in Patients After Skin Burn Injury

Stem Cell Reviews and Reports, 2011

Background Developmentally early cells, including hematopoietic stem progenitor cells (HSPCs), as... more Background Developmentally early cells, including hematopoietic stem progenitor cells (HSPCs), as well as very small embryonic-like stem cells (VSELs), are mobilized into peripheral blood (PB) in response to tissue and organ injury (e.g., heart infarct or stroke). Objective We seek to determine whether these cells are also mobilized into PB in patients with skin burn injuries. Methods Forty-four (44) patients (33-57 years of age) with total body surface burn area of 30-60%, as well as 23 healthy control subjects, were recruited and PB samples were harvested during the first 24 hours, day +2, and day +5 after burn injury and compared to normal controls. The circulating human CD34 + CD133 + cells enriched for HSPCs, as well as small CXCR4 + CD34 + CD133 + subsets of Lin-CD45cells that correspond to the population of VSELs, were counted by FACS and evaluated by direct immunofluorescence staining for pluripotency markers (Oct-4, Nanog, and SSEA-4). In parallel, we also measured by ELISA the serum concentration of factors that regulate stem cell trafficking, such as SDF-1, VEGF, and HGF. Results Our data indicate that skin burn injury mobilizes cells expressing stem cell-associated markers, such as CD133, CD34, and CXCR4, into PB. More importantly, we found an increase in the number of circulating primitive, small Oct-4 + Nanog + SSEA-4 + CXCR4 + lin-CD45-VSELs. All these changes were accompanied by increased serum concentrations of SDF-1 and VEGF.