Aneesa Rani - Academia.edu (original) (raw)

Papers by Aneesa Rani

Agricultural Science Digest - A Research Journal

Background: Mutagenesis is the process through which an organism’s genetic makeup is irreversibly... more Background: Mutagenesis is the process through which an organism’s genetic makeup is irreversibly altered. The objective of this study was to determine the optimal ethyl methanesulfonate (EMS) dosage to create desirable genetic variations in the papaya variety CO 7. Methods: The pre-soaked papaya seeds were exposed to five different concentrations of EMS (0.2%, 0.4%, 0.6%, 0.8% and 1.0%) for three hours. Non-treated seeds were used as a control. Data was recorded for germination per cent, seedling survival per cent, seedling length, girth, number of leaves, leaf length and width, petiole length and girth. Result: A declining trend in germination and growth of seedlings with the increase in EMS doses was observed. The probit curve analysis based on the seed germination percentage revealed that the LD50 value was found to be 0.55% EMS which was fixed as an optimal dose for large-scale mutagenesis experiments in papaya variety CO 7. The R2 value ranged from 0.73 to 0.99 and the growth ...

The Pharma Innovation Journal, 2021

Carica papaya Linn. Belongs to the family caricaceae and TNAU Papaya CO.8 is a dioecious, red fle... more Carica papaya Linn. Belongs to the family caricaceae and TNAU Papaya CO.8 is a dioecious, red fleshed variety with high yield and well suitable for tropical conditions and propagated through seeds. The main constraint in production is, it is dioecious in nature i.e. produces male (staminate) and female (pistillate) plants separately. Hence, grafting technology has been standardized to produce separate male and female plants for easy propagation. Though the success rate of grafts was reported to be more than 80 per cent, at times during unfavorable climatic conditions the failure in graft union occurs. Hence, in the present study GC-MS analysis was done to find out the secondary metabolites associated with the successful grafts compared to the unsuccessful grafts. The GC-MS analysis revealed that trifluoroacetoxy pentadecanal, benzoyl-2-hydroxyadamantane (Vildagliptin), styrene, Nonanal, dodecane, cyclopentylpropionic acid, tetradecane, hexdecane, hexdecanoic acid, octadecanedioic acid, pentatriacontene, tetradecynoic acid, vaccenic acid, squalene and isopropyl linoleate were the compounds present in successful grafts. Among the 15 compounds, six compounds viz., tetradecane, dodecane, styrene, hexadecane, hexadecanoic acid and nonanal were reported to possess plant growth promoting activity, which might have contributed for the successful graft union. Also organic compounds like lipid and lipid like compounds were higher in the successful grafts. Benzenoids indicated a negative impact on the graft union. This compound was observed to be present in higher amount in the unsuccessful grafts as compared to successful grafts that might have hindered graft union. A detailed study of the mode and mechanism of action of these compounds will give a clear understanding of their involvement in graft union.

The Pharma Innovation Journal, 2021

Banana (Musa spp) is one among the most important tropical fruits in terms of productivity and tr... more Banana (Musa spp) is one among the most important tropical fruits in terms of productivity and trading. Among the various banana cultivars, Ney poovan (AB) is being widely cultivated because of its sweet and sub acid flavour of the fruit. Cultivation and productivity of Ney poovan (AB) is seriously threatened by Fusarium wilt caused by destructive soil borne fungi Fusarium oxysporum f. sp. cubense (Foc). In order to assess the virulence of pathogen, in the present study, we characterized the fusarium wilt pathogen collected from four different banana growing regions of Tamil Nadu. PCR amplification of Fusarium genomic DNA with Internal Transcribed Spacer region 1 and 4 resulted that all four isolates were confirmed as Fusarium oxysporum f. sp. cubense (Foc). In order to test verify the antifungal efficacy of select bacterial endophytes viz., Brachybacterium paraconglomeratum YEBPT2 (MK263736), Brucella melitensis YEBPS3 (MN022548), Bacillus velezensis YEBBR6 (MT372157), Bacillus amyloliquefaciens YEBFL3 (MT326231) and Myroides odaratimimus YEBRT3 (MN082530) of banana against Foc, in vitro screening was performed by dual plate method. The bacterial endophyte, Brachybacterium paraconglomeratum YEBPT2 significantly suppress the mycelial growth of Foc up to 61.56 per cent and produce diverse number of antifungal bioactive metabolites like Fluorouracil

Madras Agricultural Journal, 2022

Mango (Mangifera indica. L) is characterized by a high level of genetic variation in seedling pop... more Mango (Mangifera indica. L) is characterized by a high level of genetic variation in seedling population. Estimation of genetic advance is required for the expected genotypic progress of a particular character. Keeping this in view, genetic advances of year-round flowering mango (Mangifera indica. L) genotypes was carried out during 2019-2021 in farmer’s field at Annur, Coimbatore. High Genotypic Coefficient of Variation (GCV) was found in fruit yield per tree was 30.63 per cent. The low GCV values were found for the traits viz., panicle length (6.61 %), days taken for flowering to fruit set (1.75%), days taken for flowering to fruit maturation (1.76 %), percentage of hermaphrodite flowers (5.38 %), sex ratio (5.18%). Whereas high values of heritability estimates were obtained for the traits viz., fruit yield per tree (99.43 %), number of panicles/tree (98.91 %), number of fruits per tree (97.74 %), panicle per sq.m of canopy (97.40 %). The high heritability along with high genetic ...

I International Symposium on Cashew Nut, 2015

Cashew (Anacardium occidentale L.) is a native of eastern Brazil. Four centuries ago, the adventu... more Cashew (Anacardium occidentale L.) is a native of eastern Brazil. Four centuries ago, the adventurous Portuguese came sailing down the Indian coasts and brought with them the priceless tree nut “cashew”. Cashew came, conquered and took deep roots in the entire coastal region of India. India is the leading country in the world in cashew area (9.23 lakh ha) and production (6.13 lakh tons). Cultivation of cashew in India is confined mainly to the peninsular areas. It is grown in Kerala, Karnataka, Goa and Maharashtra along the west coast and Tamil Nadu, Andhra Pradesh, Orissa and West Bengal along the east coast. To a limited extent it is being cultivated in Chattisgarh, North Eastern States (Assam, Manipur, Tripura, Meghalaya and Nagaland) and Andaman and Nicobar Islands. In Tamil Nadu, cashew is being cultivated in Ariyaloor, Cuddalore, Pudukottai, Sivagangai and Theni Districts covering an area of 1.33 lakh ha with a production of 60000 metric tons. Early attempts for cashew germplasm collection were made during the early 1950s in the composite states of Madras, Travancore, Cochin and Bombay. The research work in cashew at Tamil nadu was initiated in 1963 with the establishment of the Cashew Research Station at Vridhachalam. Research work continued under The All India Coordinated Research Project on Cashew from 1970. At the Regional Research Station, Vridhachalam cashew germplasm collection has been made since 1963. The accessions were collected from forest plantations, farmers field and research stations of Tamil Nadu, Kerala, Maharashtra and few from other countries. As a result 265 germplasm accessions were being conserved in the Regional Cashew Field Gene Bank (RCFGB) of the Regional Research Station, Vridhachalam.

Current Science, 2021

An experiment was conducted using jamun to assess the physiology of seedless fruit formation. See... more An experiment was conducted using jamun to assess the physiology of seedless fruit formation. Seedless jamuns have higher content of gibberellic acid (GA 3) (0.876 mg g-1) than seeded fruits (0.461 mg g-1) produced in the seedless genotype. Consequently, GA 3 at 100 ppm was sprayed over the jamun trees that resulted in 99.5% seedless fruit production, which was higher than the unsprayed control (72.6%). Also, the application of GA 3 at 300 ppm resulted in 36.4% seedless fruit formation in the seeded genotype. In corroboration with embryo abortion by GA 3 , the fruits sprayed with the latter at 100 ppm were analysed for their GA 3 content. The results indicated that GA 3 content was highest (5.609 mg g-1) in these fruits when compared to control (0.683 mg g-1).

International Journal of Plant & Soil Science

Avocado (Persea americana Mill.) is one of the world's most important subtropical fruit crops... more Avocado (Persea americana Mill.) is one of the world's most important subtropical fruit crops. So far, it’s collection, systematic evaluation and utilization studies are limited and this would be perhaps the first systematic study to quantify the variability available. This investigation of genetic diversity would be a first in Tamil Nadu. Morphological diversity in qualitative and quantitative traits of avocado genotypes investigated in this study using the International Plant Genetic Resources Institute descriptors to evaluate twenty accessions. The traits evaluated were related to leaf, flowering, fruit, seed, and yield traits. The 20 genotypes were classified into ten groups using Mahalanobis statistics. PA-56 and PA-68, which differ in terms of characteristics and distance, might provide transgressive segregants upon curing to resistance desirable traits. Principal component analysis was done to assess the qualitative and quantitative characters combination. The total varia...

A field experiment was conducted during 2011-2012 at Regional Research Station, TNAU, Vriddhachal... more A field experiment was conducted during 2011-2012 at Regional Research Station, TNAU, Vriddhachalam. The experiment was laid out in split plot design with main plot consisted of tertiary shoot pruning (M 1 ) and control (M2). The sub plot consisted of foliar spray of NPK 19:19:19 (1%) was given at new flush stage uniformly in all the treatments except the control in M 1 S 9 and absolute control in M 2 S 9 (August). A second spray of NPK 19:19:19 (1%), boron (0.1%), sulphate of potash (SOP) (2 %), mono ammonium phosphate (MAP) (1%) alone or in different combinations were given at flowering stage (December). The results also revealed that the treatment (M 1 S 8 ) consisting of tertiary shoot pruning along with two foliar sprays viz., NPK 19:19:19 @ one per cent at new flush stage (August) followed by a second spray of boron 0.1% + SOP 2% + MAP 1% at flowering stage (December) was found to be the best in increasing number of current season’s shoot (3.25 Numbers), number of panicles (16...

Indian Journal of Horticulture, 2010

Gymnemic acid (GA) is the principle constituent in Gymnema sylvestre, which is used in control of... more Gymnemic acid (GA) is the principle constituent in Gymnema sylvestre, which is used in control of diabetics. Standardization of protocol for in-vitro culture and suspension culture to optimize the GA production was experimented. Gymnemic acid in yellowish-brown calli was 251.73 mg 100 g−1 dw when compared to 26.31 mg 100 g−1 dw in grey calli, which was mainly due to accumulation of gymnemic acid during culture. The cells were run in suspension culture at 120 rpm and sub-cultured every 20 days. The cell density, dry weight of cells, packed cell volume and viability of cells were the highest on day 15 irrespective of the treatments. The doubling time of cells was three days. The highest gymnemic acid was recorded in auxin-free medium (579.2 mg 100 ml−1) followed by sucrose 60 g l−1 (487.96 mg 100 ml −1) and mannitol (439.66 mg 100 ml−1). The fluorescent nature of gymnemic acid was identified and photo-micrographed.

Hybridization and selection experiment was carried out in cashew to evolve a compact or dwarf F1 ... more Hybridization and selection experiment was carried out in cashew to evolve a compact or dwarf F1 hybrid suitable for high density planting system, which is the utmost need to improve the productivity of cashew nuts in the country. Eight promising hybrids with tall, semi-tall or dwarf stature were selected from the crosses and evaluated for morphological parameters and stomatal density. The results revealed that the hybrids HC 6 was dwarf with 3.0 m height, reduced number of primary and secondary branches, number of leaves per current season's shoot (5.3), reduced internodal length (0.25 cm) and number of internodes (5.3). HC 6 also recorded a higher number of stomata (57.2 per mm2). The highest cumulative yield for three years was recorded in HC 1 (3.5 kg nuts per tree) followed by HC 6 (2.65 kg nuts per tree). HC 1 was semi-tall and compact. Correlation studies revealed that there is strong positive correlation between plant height and length of current season's shoot, numb...

Journal of Pharmacognosy and Phytochemistry

International Journal of Current Microbiology and Applied Sciences

I International Symposium on Cashew Nut, 2015

I International Symposium on Cashew Nut, 2015

Agricultural Science Digest - A Research Journal

Background: Mutagenesis is the process through which an organism’s genetic makeup is irreversibly... more Background: Mutagenesis is the process through which an organism’s genetic makeup is irreversibly altered. The objective of this study was to determine the optimal ethyl methanesulfonate (EMS) dosage to create desirable genetic variations in the papaya variety CO 7. Methods: The pre-soaked papaya seeds were exposed to five different concentrations of EMS (0.2%, 0.4%, 0.6%, 0.8% and 1.0%) for three hours. Non-treated seeds were used as a control. Data was recorded for germination per cent, seedling survival per cent, seedling length, girth, number of leaves, leaf length and width, petiole length and girth. Result: A declining trend in germination and growth of seedlings with the increase in EMS doses was observed. The probit curve analysis based on the seed germination percentage revealed that the LD50 value was found to be 0.55% EMS which was fixed as an optimal dose for large-scale mutagenesis experiments in papaya variety CO 7. The R2 value ranged from 0.73 to 0.99 and the growth ...

The Pharma Innovation Journal, 2021

Carica papaya Linn. Belongs to the family caricaceae and TNAU Papaya CO.8 is a dioecious, red fle... more Carica papaya Linn. Belongs to the family caricaceae and TNAU Papaya CO.8 is a dioecious, red fleshed variety with high yield and well suitable for tropical conditions and propagated through seeds. The main constraint in production is, it is dioecious in nature i.e. produces male (staminate) and female (pistillate) plants separately. Hence, grafting technology has been standardized to produce separate male and female plants for easy propagation. Though the success rate of grafts was reported to be more than 80 per cent, at times during unfavorable climatic conditions the failure in graft union occurs. Hence, in the present study GC-MS analysis was done to find out the secondary metabolites associated with the successful grafts compared to the unsuccessful grafts. The GC-MS analysis revealed that trifluoroacetoxy pentadecanal, benzoyl-2-hydroxyadamantane (Vildagliptin), styrene, Nonanal, dodecane, cyclopentylpropionic acid, tetradecane, hexdecane, hexdecanoic acid, octadecanedioic acid, pentatriacontene, tetradecynoic acid, vaccenic acid, squalene and isopropyl linoleate were the compounds present in successful grafts. Among the 15 compounds, six compounds viz., tetradecane, dodecane, styrene, hexadecane, hexadecanoic acid and nonanal were reported to possess plant growth promoting activity, which might have contributed for the successful graft union. Also organic compounds like lipid and lipid like compounds were higher in the successful grafts. Benzenoids indicated a negative impact on the graft union. This compound was observed to be present in higher amount in the unsuccessful grafts as compared to successful grafts that might have hindered graft union. A detailed study of the mode and mechanism of action of these compounds will give a clear understanding of their involvement in graft union.

The Pharma Innovation Journal, 2021

Banana (Musa spp) is one among the most important tropical fruits in terms of productivity and tr... more Banana (Musa spp) is one among the most important tropical fruits in terms of productivity and trading. Among the various banana cultivars, Ney poovan (AB) is being widely cultivated because of its sweet and sub acid flavour of the fruit. Cultivation and productivity of Ney poovan (AB) is seriously threatened by Fusarium wilt caused by destructive soil borne fungi Fusarium oxysporum f. sp. cubense (Foc). In order to assess the virulence of pathogen, in the present study, we characterized the fusarium wilt pathogen collected from four different banana growing regions of Tamil Nadu. PCR amplification of Fusarium genomic DNA with Internal Transcribed Spacer region 1 and 4 resulted that all four isolates were confirmed as Fusarium oxysporum f. sp. cubense (Foc). In order to test verify the antifungal efficacy of select bacterial endophytes viz., Brachybacterium paraconglomeratum YEBPT2 (MK263736), Brucella melitensis YEBPS3 (MN022548), Bacillus velezensis YEBBR6 (MT372157), Bacillus amyloliquefaciens YEBFL3 (MT326231) and Myroides odaratimimus YEBRT3 (MN082530) of banana against Foc, in vitro screening was performed by dual plate method. The bacterial endophyte, Brachybacterium paraconglomeratum YEBPT2 significantly suppress the mycelial growth of Foc up to 61.56 per cent and produce diverse number of antifungal bioactive metabolites like Fluorouracil

Madras Agricultural Journal, 2022

Mango (Mangifera indica. L) is characterized by a high level of genetic variation in seedling pop... more Mango (Mangifera indica. L) is characterized by a high level of genetic variation in seedling population. Estimation of genetic advance is required for the expected genotypic progress of a particular character. Keeping this in view, genetic advances of year-round flowering mango (Mangifera indica. L) genotypes was carried out during 2019-2021 in farmer’s field at Annur, Coimbatore. High Genotypic Coefficient of Variation (GCV) was found in fruit yield per tree was 30.63 per cent. The low GCV values were found for the traits viz., panicle length (6.61 %), days taken for flowering to fruit set (1.75%), days taken for flowering to fruit maturation (1.76 %), percentage of hermaphrodite flowers (5.38 %), sex ratio (5.18%). Whereas high values of heritability estimates were obtained for the traits viz., fruit yield per tree (99.43 %), number of panicles/tree (98.91 %), number of fruits per tree (97.74 %), panicle per sq.m of canopy (97.40 %). The high heritability along with high genetic ...

I International Symposium on Cashew Nut, 2015

Cashew (Anacardium occidentale L.) is a native of eastern Brazil. Four centuries ago, the adventu... more Cashew (Anacardium occidentale L.) is a native of eastern Brazil. Four centuries ago, the adventurous Portuguese came sailing down the Indian coasts and brought with them the priceless tree nut “cashew”. Cashew came, conquered and took deep roots in the entire coastal region of India. India is the leading country in the world in cashew area (9.23 lakh ha) and production (6.13 lakh tons). Cultivation of cashew in India is confined mainly to the peninsular areas. It is grown in Kerala, Karnataka, Goa and Maharashtra along the west coast and Tamil Nadu, Andhra Pradesh, Orissa and West Bengal along the east coast. To a limited extent it is being cultivated in Chattisgarh, North Eastern States (Assam, Manipur, Tripura, Meghalaya and Nagaland) and Andaman and Nicobar Islands. In Tamil Nadu, cashew is being cultivated in Ariyaloor, Cuddalore, Pudukottai, Sivagangai and Theni Districts covering an area of 1.33 lakh ha with a production of 60000 metric tons. Early attempts for cashew germplasm collection were made during the early 1950s in the composite states of Madras, Travancore, Cochin and Bombay. The research work in cashew at Tamil nadu was initiated in 1963 with the establishment of the Cashew Research Station at Vridhachalam. Research work continued under The All India Coordinated Research Project on Cashew from 1970. At the Regional Research Station, Vridhachalam cashew germplasm collection has been made since 1963. The accessions were collected from forest plantations, farmers field and research stations of Tamil Nadu, Kerala, Maharashtra and few from other countries. As a result 265 germplasm accessions were being conserved in the Regional Cashew Field Gene Bank (RCFGB) of the Regional Research Station, Vridhachalam.

Current Science, 2021

An experiment was conducted using jamun to assess the physiology of seedless fruit formation. See... more An experiment was conducted using jamun to assess the physiology of seedless fruit formation. Seedless jamuns have higher content of gibberellic acid (GA 3) (0.876 mg g-1) than seeded fruits (0.461 mg g-1) produced in the seedless genotype. Consequently, GA 3 at 100 ppm was sprayed over the jamun trees that resulted in 99.5% seedless fruit production, which was higher than the unsprayed control (72.6%). Also, the application of GA 3 at 300 ppm resulted in 36.4% seedless fruit formation in the seeded genotype. In corroboration with embryo abortion by GA 3 , the fruits sprayed with the latter at 100 ppm were analysed for their GA 3 content. The results indicated that GA 3 content was highest (5.609 mg g-1) in these fruits when compared to control (0.683 mg g-1).

International Journal of Plant & Soil Science

Avocado (Persea americana Mill.) is one of the world's most important subtropical fruit crops... more Avocado (Persea americana Mill.) is one of the world's most important subtropical fruit crops. So far, it’s collection, systematic evaluation and utilization studies are limited and this would be perhaps the first systematic study to quantify the variability available. This investigation of genetic diversity would be a first in Tamil Nadu. Morphological diversity in qualitative and quantitative traits of avocado genotypes investigated in this study using the International Plant Genetic Resources Institute descriptors to evaluate twenty accessions. The traits evaluated were related to leaf, flowering, fruit, seed, and yield traits. The 20 genotypes were classified into ten groups using Mahalanobis statistics. PA-56 and PA-68, which differ in terms of characteristics and distance, might provide transgressive segregants upon curing to resistance desirable traits. Principal component analysis was done to assess the qualitative and quantitative characters combination. The total varia...

A field experiment was conducted during 2011-2012 at Regional Research Station, TNAU, Vriddhachal... more A field experiment was conducted during 2011-2012 at Regional Research Station, TNAU, Vriddhachalam. The experiment was laid out in split plot design with main plot consisted of tertiary shoot pruning (M 1 ) and control (M2). The sub plot consisted of foliar spray of NPK 19:19:19 (1%) was given at new flush stage uniformly in all the treatments except the control in M 1 S 9 and absolute control in M 2 S 9 (August). A second spray of NPK 19:19:19 (1%), boron (0.1%), sulphate of potash (SOP) (2 %), mono ammonium phosphate (MAP) (1%) alone or in different combinations were given at flowering stage (December). The results also revealed that the treatment (M 1 S 8 ) consisting of tertiary shoot pruning along with two foliar sprays viz., NPK 19:19:19 @ one per cent at new flush stage (August) followed by a second spray of boron 0.1% + SOP 2% + MAP 1% at flowering stage (December) was found to be the best in increasing number of current season’s shoot (3.25 Numbers), number of panicles (16...

Indian Journal of Horticulture, 2010

Gymnemic acid (GA) is the principle constituent in Gymnema sylvestre, which is used in control of... more Gymnemic acid (GA) is the principle constituent in Gymnema sylvestre, which is used in control of diabetics. Standardization of protocol for in-vitro culture and suspension culture to optimize the GA production was experimented. Gymnemic acid in yellowish-brown calli was 251.73 mg 100 g−1 dw when compared to 26.31 mg 100 g−1 dw in grey calli, which was mainly due to accumulation of gymnemic acid during culture. The cells were run in suspension culture at 120 rpm and sub-cultured every 20 days. The cell density, dry weight of cells, packed cell volume and viability of cells were the highest on day 15 irrespective of the treatments. The doubling time of cells was three days. The highest gymnemic acid was recorded in auxin-free medium (579.2 mg 100 ml−1) followed by sucrose 60 g l−1 (487.96 mg 100 ml −1) and mannitol (439.66 mg 100 ml−1). The fluorescent nature of gymnemic acid was identified and photo-micrographed.

Hybridization and selection experiment was carried out in cashew to evolve a compact or dwarf F1 ... more Hybridization and selection experiment was carried out in cashew to evolve a compact or dwarf F1 hybrid suitable for high density planting system, which is the utmost need to improve the productivity of cashew nuts in the country. Eight promising hybrids with tall, semi-tall or dwarf stature were selected from the crosses and evaluated for morphological parameters and stomatal density. The results revealed that the hybrids HC 6 was dwarf with 3.0 m height, reduced number of primary and secondary branches, number of leaves per current season's shoot (5.3), reduced internodal length (0.25 cm) and number of internodes (5.3). HC 6 also recorded a higher number of stomata (57.2 per mm2). The highest cumulative yield for three years was recorded in HC 1 (3.5 kg nuts per tree) followed by HC 6 (2.65 kg nuts per tree). HC 1 was semi-tall and compact. Correlation studies revealed that there is strong positive correlation between plant height and length of current season's shoot, numb...

Journal of Pharmacognosy and Phytochemistry

International Journal of Current Microbiology and Applied Sciences

I International Symposium on Cashew Nut, 2015

I International Symposium on Cashew Nut, 2015