Angelovski Ljupco - Academia.edu (original) (raw)

Uploads

Papers by Angelovski Ljupco

The infl uence of three organic acid, (acetic, lactic and citric acid in diff erent concentration... more The infl uence of three organic acid, (acetic, lactic and citric acid in diff erent concentrations) were evaluated over two major foodborne pathogens, Listeria monocytogenes and Salmonella enteritidis. For evaluation we used two diff erent laboratory designed models such as, agar gel diff usion test and broth reduction test. The results from agar gel diff usion tests revealed variation of the inhibition zone of Listeria monocytogenes and Salmonella enteritidis to three engaged organic acids in concentration 10%, 8%, 6%, 4%, 2%. The two tested organisms were most sensitive to lactic acid and slightly less sensitive to citric and acetic acid respectively. Listeria monocytogenes was more sensitive to all three tested organic acids compare to Salmonella enteritidis. Infl uence of above mentioned organic acids in buff ered peptone water broths revealed Listeria monocytogenes reduction in cell number by 4,6 log cfu/mL for 0.04% acetic acid and 3,1 and 2.8 log cfu/mL for lactic acid and ac...

In the period from October to December 2011, 61 isolates of Salmonella were isolated from fl ocks... more In the period from October to December 2011, 61 isolates of Salmonella were isolated from fl ocks of laying hens providing eggs for human consumption. Most of the isolates belonged to the serovars Enteritidis (n=35; 57.38%), Typhimurium (n=17; 27.87%) and other (n=9; 14.75%). These isolates were tested for antimicrobial resistance to ampicillin, cefotaxime, chloramphenicol, ciprofl oxacin, nalidixic acid, sulfamethoxazole, tetracycline, gentamicin and streptomycin. All of the tested Salmonella were sensitive to cefotaxime, chloramphenicol and ciprofl oxacin. The highest resistance rate was shown to tetracycline with eight (13,11%) and streptomycin with nine (14,75%) isolates, three (4,92%) isolates were resistant to nalidixic acid, and one isolate (1,63%) exhibited resistance to ampicillin, sulfamethoxazole and gentamicin. Forty four isolates (72,13%) were sensitive to all antimicrobials. i.e. 17 (27,87%) isolates showed resistance to at least one antimicrobial of which 12 isolates showed resistance to one, four isolates showed resistance to two antimicrobials and one isolate showed resistance on three antimicrobials. When results of resistance levels of Salmonella spp. were compared with the offi cial data from the European Union reported by EFSA, it was concluded that the Salmonella spp. isolated from fl ocks of laying hens providing eggs for human consumption in R. Macedonia have low resistance to antimicrobial drugs.

Pathogenic bacteria in milk have been an issue of public health concern since the early days of t... more Pathogenic bacteria in milk have been an issue of public health concern since the early days of the dairy industry. Many diseases such as tuberculosis, brucellosis, diphtheria, Scarlet fever, Q-fever and gastroenteritis are transmissible via milk and

There is a potential risk of contamination with pathogen bacteria during the food processing, pac... more There is a potential risk of contamination with pathogen bacteria during the food processing, packaging, storage, and transportation but it is possible to minimize the contamination if sanitary measures, hygiene practices, proper cooling, careful handling and proper storage of the products is enforced. However, there are still cases of outbreaks UDC: 579.64:637.5(497.7)

The purpose of this research was focused on determination of the concentrations of activity of 22... more The purpose of this research was focused on determination of the concentrations of activity of 226 Ra, 232 Th, 40 К and 137 Cs in the soil samples collected from cultivated soil close to several larger cities in the Republic of Macedonia (Tetovo, Veles, Bitola) by using gamma spectrometry. Data show that the average value of activity of 226 Ra and 232 Th are within the range of (25.58-36.48) and (36.55-50.45) Bq kg -1, respectively. While, the concentrations of activity of 40 К in comparison to those obtained for 232 Th and 226 Ra are higher in all analyzed soils samples, showing values within the range of (611.59-781.23) Bq kg -1, the activities of 137 Cs are generally lower and are within the range of (9.33-18.90) Bq kg -1, with the highest activity at a depth of 5 cm, which is well in accordance with the data from the literature. However, 40 К is the only radionuclide which was determined with a significant quantity in the soil samples, and for which one can tell that in all loca...

Mastitis is probably the most common and costly disease in modern dairy cow husbandry. The aim of... more Mastitis is probably the most common and costly disease in modern dairy cow husbandry. The aim of the present paper was to report the results concerning udder health after implementation of a specific strategy using both field and laboratory methods. During the period June 2010-December 2011 a total of 674 dairy cows from four dairy farms were included in the investigation. Clinical mastitis was diagnosed by detection of signs of inflammation in the udder, while subclinical mastitis was diagnosed at the animal level by an increased Somatic Cell Count (SCC) using laboratory tests, and subsequently confirmed at quarter level by California Mastitis Test (CMT). Microbiological analysis of the milk samples was carried out by standard procedures using Gram staining, biochemical tests and automated identification system. The distribution of somatic cell counts on cow level (n=674) was:305 (45.3%) with SCC less than 100,000SCC/mL, 236 (35.0%) 100,001 - 350,000 SCC/mL, and 133 (18.7%) with m...