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Papers by Anildo Teixeira MOntes Junior

Research paper thumbnail of Investigation of glutathione peroxidase activity in chicken meat under different experimental conditions

Food Science and Technology (Campinas), 2012

Due to the fact that previous studies on the enzymatic activity of Glutathione peroxidase (GSH-Px... more Due to the fact that previous studies on the enzymatic activity of Glutathione peroxidase (GSH-Px) diverge widely in their methodology and results, this study aimed to investigate the influence of different analytical conditions on GSH-Px activity in chicken thighs from broilers that were fed different diets with different sources and concentrations of selenium. GSH-Px activity was evaluated six hours after slaughter and 120 days after frozen storage at -18 ºC. The different analytical conditions included time of pre-incubation (0, 10 and 30 minutes), reaction medium, types of substrate (H2O2 (0.72 mM, 7.2 mM, and 72 mM) and Terc-butil hydroperoxide 15 mM), and different buffer concentrations (buffer 1, potassium phosphate 50 mM pH 7.0 + EDTA 1 mM + mercaptoethanol 1 mM, and buffer 2, tris-HCl 50 mM pH 7.6 + EDTA 1 mM + mercapthanol 5 mM). The results show that the highest GSH-Px activity was observed when enzyme and substrate were in contact at 22 ºC without any pre-incubation, and...

Research paper thumbnail of Phytogenic additive as an alternative to growth promoters in broiler chickens

Ciência Rural, 2009

This study evaluated a phytogenic feed additive for broiler chickens. A total of 1,632 broiler ch... more This study evaluated a phytogenic feed additive for broiler chickens. A total of 1,632 broiler chicks were distributed into four treatments: negative control (without growth promoter); positive control (avilamycine, 10ppm + colistin, 15ppm); and two alternative treatments with 150ppm of phytogenic additive, one with a reduced Ca and P levels diet (PA-R1) and the other with lower energy, and amino acids, besides Ca and P (PA-R2). The trial was conducted with 12 replicates, each consisted of a pen with 34 birds. The alternative diets showed body weight intermediate to the two controls at 42 days, with no significant (P>0.05) treatment effect on feed conversion ratio. No treatment differences (P>0.05) on carcass yield and composition was observed. There was a tendency of abdominal fat lipids saturation, when the phytogenic additive was used, as possible consequence of a decreased level of soybean oil in the diets. A difference (P<0.001) on ingredient consumption profile was ob...

Research paper thumbnail of Antinociceptive properties of coumarins, steroid and dihydrostyryl-2-pyrones from Polygala sabulosa (Polygalaceae) in mice

Journal of Pharmacy and Pharmacology, 2006

We have investigated the possible antinociceptive action of the extract, fractions and pure compo... more We have investigated the possible antinociceptive action of the extract, fractions and pure compounds obtained from the whole plant Polygala sabulosa A. W. Bennett (Polygalaceae) in acetic acid-induced visceral pain in mice. Intraperitoneal injection of animals with the hydroalcoholic extract and fractions (CH 2 Cl 2 , EtOAc, n-BuOH, aqueous fraction) (1-100 mg kg − 1) caused a doserelated and significant inhibition of the acetic acid-induced visceral nociceptive response. The CH 2 Cl 2 , EtOAc and n-BuOH fractions were more potent than the hydroalcoholic extract and aqueous fraction. The isolated compounds dihydrostyryl-2-pyrones (1, 2, 3), styryl-2-pyrone (7), ␣-spinasterol (9), scopoletin (10) and two esters of the coumarin (scopoletin) obtained semisynthetically, acetylscopoletin (10a) and benzoylscopoletin (10b) (0.001-10 mg kg − 1), exhibited significant and doserelated antinociceptive effects against acetic acid-induced visceral pain. The results distinguished, for the first time, the extract, fractions and pure compounds obtained from P. sabulosa that produced marked antinociception against the acetic acid-induced visceral nociceptive response, supporting the ethnomedical use of P. sabulosa. Polygala sabulosa A. W. Bennett (Polygalaceae), popularly known as 'timutu-pinheirinho', is a small herb growing in the Southern Meridional Highlands of Brazil (Wurdack & Smith 1971). The plants of the genus Polygala are used in folk medicine for the treatment of several pathologies, including disorders of the bowel and kidney, as a tonic remedy, and as a topical anaesthetic and expectorant (Wasicky 1945). Apart from these medicinal uses, there are reports showing antiviral (Cyong et al 2000), trypanocidal (Pizzolatti et al 2003), tumour inhibitor (Mak et al 2001), hypoglycaemic (Kako et al 1996) and neuroprotective (Kwon et al 2004) effects of some Polygala species. We have reported previously that the hydroalcoholic extract and xanthones obtained from P. cyparissias elicited pronounced antinociceptive effects in chemical and mechanical nociception models (De Campos et al 1997). In addition, several species of plants belonging to the genus Polygala, including P. cyparissias, P. linoides, P. campestris, P. paniculata and P. aspalata, are extensively distributed in Santa Catarina. These plants contain high concentrations of methyl salicylate, which is responsible for a characteristic odour of their roots (Wasicky 1945). Chemical studies carried out on some of the species belonging to the genus Polygala have demonstrated the presence of many classes of constituents, such as coumarins, saponins, lignans, flavonoids and mainly xanthones (Pinheiro et al 1998; Zhang et al 1998; Cristiano et al 2003). More recently, Pizzolatti et al (2000, 2004) have reported the isolation of 7-prenyloxy-6-methoxycoumarin, photohypericin, four styryl-2-pyrones and three dihydrostyryl-2-pyrones from P. sabulosa. However, to date no pharmacological study has been carried out on this species. Furthermore, whole plant from P. sabulosa is used in folk medicine; hence, this study has examined the possible antinociceptive action of the hydroalcoholic extract, fractions and isolated compounds in the acetic acid-induced visceral nociceptive response, a classical chemical model of nociception, in mice.

Research paper thumbnail of Effect of crude extract and fractions from Vitex megapotamica leaves on hyperglycemia in alloxan-diabetic rats

Journal of Ethnopharmacology, 2007

The effect of the crude extract, ethyl acetate and n-butanol fractions from Vitex megapotamica (S... more The effect of the crude extract, ethyl acetate and n-butanol fractions from Vitex megapotamica (Spreng) Moldenke on glycemia was investigated in diabetic rats. Oral administration of crude extract significantly reduced serum glucose levels in both normal and diabetic animals. In normal rats, serum glucose lowering was observed with 400 and 800 mg/kg at 2 and 2-3 h, respectively after oral crude extract treatment. Nevertheless, the hypoglycemic effect of Vitex megapotamica in diabetic rats was evident at 1 and 2 h and from 1 to 3 h after treatment with 400 and 800 mg/kg, respectively. The ethyl acetate as well as n-butanol fractions were able to diminish glycemia in diabetic animals. The ethyl acetate fraction (400 and 800 mg/kg) produced the maximum hypoglycemic effect (28 and 20%, respectively) in diabetic rats and the same dose of the n-butanol fraction reduced the hyperglycemia only by 11% at 1 h after treatment. Additionally, in hyperglycemic normal rats neither crude extract nor ethyl acetate fraction modified the glucose tolerance and the known tolbutamide effect on insulin release was clearly observed in this group. Thus, this study shows that Vitex megapotamica has an anti-hyperglycemic action, is able to ameliorate the diabetic state and, probably, is a source of hypoglycemic compounds.

Research paper thumbnail of Lactobacillus plantarum AJ2 isolated from naturally fermented sausage and its effects on the technological properties of Milano-type salami

Ciência e Tecnologia de Alimentos, 2008

Lactobacillus plantarum AJ2 isolado de salame naturalmente fermentado e seus efeitos nas propried... more Lactobacillus plantarum AJ2 isolado de salame naturalmente fermentado e seus efeitos nas propriedades tecnológicas do salame tipo Milano

Research paper thumbnail of Influence of a native strain of Staphylococcus xylosus on the microbiological, physicochemical and sensorial characteristics on milano salami type

Brazilian Archives of Biology and Technology, 2010

In this work, the influence of native starter cultures on the microbiological, physicochemical an... more In this work, the influence of native starter cultures on the microbiological, physicochemical and sensorial characteristics of Milano salami type was studied. Two batches of Milano salami type were produced: Batch A, with the addition of Staphylococcus xylosus U5 and Batch B (control) without the starter culture. The Milano salami type was characterized by an important microbial activity of coagulase-negative staphylococci (CNS) that resulted in substantial growth in Batch A during the ripening with an initial count of 7.60 log cfu.g-1 and reached 9.84 log CFU.g-1 after 14 days. Bacterial enzymes that showed efficient activity under the conditions found in Milano salami type were catalase, nitrite and nitrate reductase, contributing for sensory and physicochemical properties of the product. There were no significant differences in general free fatty acids composition among the batches, while the color parameters (L *, a * and b *) in the Batch A presented significantly higher value...

Research paper thumbnail of Hope for insulin mimetic oral antidiabetic drugs Eur J Endocrinol 141 (6) 561-562, doi: 10.1530/eje. 0.1410561

Research paper thumbnail of Effect of crude extract and fractions from< i> Vitex megapotamica</i> leaves on hyperglycemia in alloxan-diabetic rats

Research paper thumbnail of Investigation of glutathione peroxidase activity in chicken meat under different experimental conditions

Food Science and Technology (Campinas), 2012

Due to the fact that previous studies on the enzymatic activity of Glutathione peroxidase (GSH-Px... more Due to the fact that previous studies on the enzymatic activity of Glutathione peroxidase (GSH-Px) diverge widely in their methodology and results, this study aimed to investigate the influence of different analytical conditions on GSH-Px activity in chicken thighs from broilers that were fed different diets with different sources and concentrations of selenium. GSH-Px activity was evaluated six hours after slaughter and 120 days after frozen storage at -18 ºC. The different analytical conditions included time of pre-incubation (0, 10 and 30 minutes), reaction medium, types of substrate (H2O2 (0.72 mM, 7.2 mM, and 72 mM) and Terc-butil hydroperoxide 15 mM), and different buffer concentrations (buffer 1, potassium phosphate 50 mM pH 7.0 + EDTA 1 mM + mercaptoethanol 1 mM, and buffer 2, tris-HCl 50 mM pH 7.6 + EDTA 1 mM + mercapthanol 5 mM). The results show that the highest GSH-Px activity was observed when enzyme and substrate were in contact at 22 ºC without any pre-incubation, and...

Research paper thumbnail of Phytogenic additive as an alternative to growth promoters in broiler chickens

Ciência Rural, 2009

This study evaluated a phytogenic feed additive for broiler chickens. A total of 1,632 broiler ch... more This study evaluated a phytogenic feed additive for broiler chickens. A total of 1,632 broiler chicks were distributed into four treatments: negative control (without growth promoter); positive control (avilamycine, 10ppm + colistin, 15ppm); and two alternative treatments with 150ppm of phytogenic additive, one with a reduced Ca and P levels diet (PA-R1) and the other with lower energy, and amino acids, besides Ca and P (PA-R2). The trial was conducted with 12 replicates, each consisted of a pen with 34 birds. The alternative diets showed body weight intermediate to the two controls at 42 days, with no significant (P>0.05) treatment effect on feed conversion ratio. No treatment differences (P>0.05) on carcass yield and composition was observed. There was a tendency of abdominal fat lipids saturation, when the phytogenic additive was used, as possible consequence of a decreased level of soybean oil in the diets. A difference (P<0.001) on ingredient consumption profile was ob...

Research paper thumbnail of Antinociceptive properties of coumarins, steroid and dihydrostyryl-2-pyrones from Polygala sabulosa (Polygalaceae) in mice

Journal of Pharmacy and Pharmacology, 2006

We have investigated the possible antinociceptive action of the extract, fractions and pure compo... more We have investigated the possible antinociceptive action of the extract, fractions and pure compounds obtained from the whole plant Polygala sabulosa A. W. Bennett (Polygalaceae) in acetic acid-induced visceral pain in mice. Intraperitoneal injection of animals with the hydroalcoholic extract and fractions (CH 2 Cl 2 , EtOAc, n-BuOH, aqueous fraction) (1-100 mg kg − 1) caused a doserelated and significant inhibition of the acetic acid-induced visceral nociceptive response. The CH 2 Cl 2 , EtOAc and n-BuOH fractions were more potent than the hydroalcoholic extract and aqueous fraction. The isolated compounds dihydrostyryl-2-pyrones (1, 2, 3), styryl-2-pyrone (7), ␣-spinasterol (9), scopoletin (10) and two esters of the coumarin (scopoletin) obtained semisynthetically, acetylscopoletin (10a) and benzoylscopoletin (10b) (0.001-10 mg kg − 1), exhibited significant and doserelated antinociceptive effects against acetic acid-induced visceral pain. The results distinguished, for the first time, the extract, fractions and pure compounds obtained from P. sabulosa that produced marked antinociception against the acetic acid-induced visceral nociceptive response, supporting the ethnomedical use of P. sabulosa. Polygala sabulosa A. W. Bennett (Polygalaceae), popularly known as 'timutu-pinheirinho', is a small herb growing in the Southern Meridional Highlands of Brazil (Wurdack & Smith 1971). The plants of the genus Polygala are used in folk medicine for the treatment of several pathologies, including disorders of the bowel and kidney, as a tonic remedy, and as a topical anaesthetic and expectorant (Wasicky 1945). Apart from these medicinal uses, there are reports showing antiviral (Cyong et al 2000), trypanocidal (Pizzolatti et al 2003), tumour inhibitor (Mak et al 2001), hypoglycaemic (Kako et al 1996) and neuroprotective (Kwon et al 2004) effects of some Polygala species. We have reported previously that the hydroalcoholic extract and xanthones obtained from P. cyparissias elicited pronounced antinociceptive effects in chemical and mechanical nociception models (De Campos et al 1997). In addition, several species of plants belonging to the genus Polygala, including P. cyparissias, P. linoides, P. campestris, P. paniculata and P. aspalata, are extensively distributed in Santa Catarina. These plants contain high concentrations of methyl salicylate, which is responsible for a characteristic odour of their roots (Wasicky 1945). Chemical studies carried out on some of the species belonging to the genus Polygala have demonstrated the presence of many classes of constituents, such as coumarins, saponins, lignans, flavonoids and mainly xanthones (Pinheiro et al 1998; Zhang et al 1998; Cristiano et al 2003). More recently, Pizzolatti et al (2000, 2004) have reported the isolation of 7-prenyloxy-6-methoxycoumarin, photohypericin, four styryl-2-pyrones and three dihydrostyryl-2-pyrones from P. sabulosa. However, to date no pharmacological study has been carried out on this species. Furthermore, whole plant from P. sabulosa is used in folk medicine; hence, this study has examined the possible antinociceptive action of the hydroalcoholic extract, fractions and isolated compounds in the acetic acid-induced visceral nociceptive response, a classical chemical model of nociception, in mice.

Research paper thumbnail of Effect of crude extract and fractions from Vitex megapotamica leaves on hyperglycemia in alloxan-diabetic rats

Journal of Ethnopharmacology, 2007

The effect of the crude extract, ethyl acetate and n-butanol fractions from Vitex megapotamica (S... more The effect of the crude extract, ethyl acetate and n-butanol fractions from Vitex megapotamica (Spreng) Moldenke on glycemia was investigated in diabetic rats. Oral administration of crude extract significantly reduced serum glucose levels in both normal and diabetic animals. In normal rats, serum glucose lowering was observed with 400 and 800 mg/kg at 2 and 2-3 h, respectively after oral crude extract treatment. Nevertheless, the hypoglycemic effect of Vitex megapotamica in diabetic rats was evident at 1 and 2 h and from 1 to 3 h after treatment with 400 and 800 mg/kg, respectively. The ethyl acetate as well as n-butanol fractions were able to diminish glycemia in diabetic animals. The ethyl acetate fraction (400 and 800 mg/kg) produced the maximum hypoglycemic effect (28 and 20%, respectively) in diabetic rats and the same dose of the n-butanol fraction reduced the hyperglycemia only by 11% at 1 h after treatment. Additionally, in hyperglycemic normal rats neither crude extract nor ethyl acetate fraction modified the glucose tolerance and the known tolbutamide effect on insulin release was clearly observed in this group. Thus, this study shows that Vitex megapotamica has an anti-hyperglycemic action, is able to ameliorate the diabetic state and, probably, is a source of hypoglycemic compounds.

Research paper thumbnail of Lactobacillus plantarum AJ2 isolated from naturally fermented sausage and its effects on the technological properties of Milano-type salami

Ciência e Tecnologia de Alimentos, 2008

Lactobacillus plantarum AJ2 isolado de salame naturalmente fermentado e seus efeitos nas propried... more Lactobacillus plantarum AJ2 isolado de salame naturalmente fermentado e seus efeitos nas propriedades tecnológicas do salame tipo Milano

Research paper thumbnail of Influence of a native strain of Staphylococcus xylosus on the microbiological, physicochemical and sensorial characteristics on milano salami type

Brazilian Archives of Biology and Technology, 2010

In this work, the influence of native starter cultures on the microbiological, physicochemical an... more In this work, the influence of native starter cultures on the microbiological, physicochemical and sensorial characteristics of Milano salami type was studied. Two batches of Milano salami type were produced: Batch A, with the addition of Staphylococcus xylosus U5 and Batch B (control) without the starter culture. The Milano salami type was characterized by an important microbial activity of coagulase-negative staphylococci (CNS) that resulted in substantial growth in Batch A during the ripening with an initial count of 7.60 log cfu.g-1 and reached 9.84 log CFU.g-1 after 14 days. Bacterial enzymes that showed efficient activity under the conditions found in Milano salami type were catalase, nitrite and nitrate reductase, contributing for sensory and physicochemical properties of the product. There were no significant differences in general free fatty acids composition among the batches, while the color parameters (L *, a * and b *) in the Batch A presented significantly higher value...

Research paper thumbnail of Hope for insulin mimetic oral antidiabetic drugs Eur J Endocrinol 141 (6) 561-562, doi: 10.1530/eje. 0.1410561

Research paper thumbnail of Effect of crude extract and fractions from< i> Vitex megapotamica</i> leaves on hyperglycemia in alloxan-diabetic rats