Anjana Goel - Academia.edu (original) (raw)

Papers by Anjana Goel

Frontiers in Pharmacology, Dec 4, 2023

Journal of Young Pharmacists, Aug 24, 2023

Current Topics in Medicinal Chemistry

: Depression being a common comorbidity of rheumatoid arthritis (RA) is found to be responsible f... more : Depression being a common comorbidity of rheumatoid arthritis (RA) is found to be responsible for the reduction in the lifespan of the sufferer along with the compromised quality of life. The study quoted below highlights the pathogenic pathways, the frequency of RA along with its impact on patients, thus, raising awareness about the concerned topic. It is found that the chances and frequency of developing depression are 2-3 times higher in patients with RA in comparison to the general population. For such studies, self-reported questionnaires along with proper screening of inclusion and exclusion criteria have been employed which helped in a better comparative study of the topic. As per a report from a meta-analysis, 16.8% of patients with RA have been observed to develop severe depression. According to recent research in the related field, the hypothesis of the role of immune-mediated processes and their role in brain networks and inflammation has been found to be engaged in the progression and pathophysiology of depression in patients with RA. Autoimmune mechanisms and cytokines are found to play an essential role in coordination for initiating and sustaining the disorder. Involvement of IL-1, IL-6 and TNF-α has been studied and analysed widely. A number of studies have shown a connection between depression and RA-related physical impairment, fatigue, and increased pain. Higher mortality, reduced treatment compliance, and more comorbidities effects increased suicide risk. It is also found that depression along with RA leads to hospitalizations, which in turn increase the cost of care for the patient. Hence, it could be stated that the study of depression in RA can be an important marker for the progression of RA and its prognosis. The latest treatment strategies for RA include management of symptoms and early disorder treatment The current review aims to investigate and bring the links between RA and its symptoms into the limelight, including the psycho-social, physiological, and neurological aspects along with their molecular mechanism, for a better discernment of the topic for the readers.

Reproduction in Domestic Animals, 2016

During the last decades, physiological effects of oestrogens have been increasingly explored by s... more During the last decades, physiological effects of oestrogens have been increasingly explored by scientists and biotechnologists. Estrogens exert a wide range of effects on a large variety of cell types. Oestrogen and its receptors are essential for sexual development and reproduction. Estrogen receptor alpha is a nuclear receptor activated by the hormone oestrogen. In male, ERα is encoded by the gene estrogen receptor gene 1 (ESR1), responsible for better fertility. The ESR1 is involved in the reabsorption of luminal fluid during the transit of spermatozoa from the testis to the head of the epididymis which is important for their survival and maturation during epididymal storage. The absence of ESR1 leads to reduced epididymal sperm content, reduced sperm motility and fertilizing ability. Therefore, this is a good startby to study the expression pattern of estrogen receptor 1 gene in high-fertile (G1) and low-fertile (G2) bucks of Jamunapari and Barbari breeds identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the tissues by TRIzol method. The identification and expression pattern of caprine ESR1 gene was analysed by real-time PCR (Roche LC-480). Our work shows that the relative quantification by RT-PCR indicates more fold in head of epididymis as compared to spleen of caprine ESR1 gene. Furthermore, the RT-PCR indicated that fertile bucks of Jamunapari breed have more fold value as compared to Barbari breed in respect of reproductive organ.

Small Ruminant Research, 2006

The aim of the study was to evaluate the optimum concentration of the estrous goat serum for goat... more The aim of the study was to evaluate the optimum concentration of the estrous goat serum for goat in vitro oocyte maturation. Goat ovaries were collected from a local abattoir and transported within 3h to the laboratory in a warm saline solution (30–35°C), supplemented with 100IU penicillin G and 100μg streptomycin sulfate/ml. Cumulus-oocyte complexes (COC&amp;amp;amp;#39;s) were obtained by puncture of

Journal of Ecophysiology and Occupational Health, Dec 14, 2023

Indian Journal of Small Ruminants (The)

A study was aimed to optimize the electrofusion parameters for generation of caprine tetraploid i... more A study was aimed to optimize the electrofusion parameters for generation of caprine tetraploid in vitro fertilized embryos. Freshly recovered 2-cell goat in vitro fertilized embryos were submitted to electrofusion with varying voltages (1.0, 1.2 and 1.4 kV/cm) and time duration (2, 4 and 6 μ sec) combinations to produce tetraploid goat embryos. Fused embryos were cultured in RVCL (Research vitro cleave) media further for 8 to 12 days for subsequent embryo development. Fusion, cleavage and blastocyst rate at voltage 1.2kV/cm for 4 μ sec duration was significantly higher (P<0.05) than other groups. It was also observed that less than one third of electrofused embryos bore deviation from the expected ploidy. It was concluded that optimum condition for electrofusion of goat fertilized 2-cell embryos to produce goat tetraploid embryos is 1.2 kV/cm for 4 μ sec so as to get maximum number of fused and cleaved goat embryos.

Indian Journal of Animal Sciences, Sep 10, 2020

Materials Today: Proceedings

IOP Conference Series: Materials Science and Engineering, 2021

Biomaterials can be stated as an amalgamation of science in research and development where the fi... more Biomaterials can be stated as an amalgamation of science in research and development where the field of cellular biology, molecular biology, chemical sciences, materials sciences and engineering plays important role. These are substances which have been modified to interact with biological systems for therapeutic purposes and even diagnosis. They induce a synergistic effect in the environment where implanted. Although as a science, it is years old but at present it is growing as a modern-day research platform that finds its application in many fields of medical science and cancer being the most extensively researched field in the same. Such studies undertaken in the field of biomaterials has been reviewed in the paper that includes the use of biomaterials as therapeutic agents including their use as vaccines and surface modulators to enhance the antigen specific T-cell activity in the immunotherapy for cancers. The application has been found reliable in case of recurrent cancers, po...

Journal of Infectious Diseases and Therapy, Sep 22, 2016

The enhanced availability of functional fibroblasts from precious tissue samples requires an idea... more The enhanced availability of functional fibroblasts from precious tissue samples requires an ideal cell-culture system. Therefore, this study was designed to investigate the performance of caprine adult fibroblast cells (cadFibroblast) when cultivated in different culture media. The cadFibroblast cell lines from adult Barbari bucks were established and the effect of different media viz. DMEM/F-12 [with low glucose (5.5 mM; DL) and high glucose (30 mM; DH)], α-MEM [with low glucose (5.5 mM; ML) and with high glucose (30 mM; MH)], and fibroblast growth medium (FGM) were evaluated and compared for growth characteristics and in-vitro dynamics through cellular morphology, proliferation, population doubling time, double-immunocytochemistry, colony forming units, wound healing, transwell migration and differential expression of fibroblast-specific markers [FSP-1 and vimentin]. The results of immunocytochemistry, transwell migration/invasion, and wound healing assays showed the superiority ...

Diagnosis of early leprosy is a major hurdle to disease control and has been compromised due to l... more Diagnosis of early leprosy is a major hurdle to disease control and has been compromised due to lack of specific markers or tests. As proteins are the functional moiety, circulating proteins in sera could be attractive diagnostic markers of disease. Our knowledge regarding the proteome of contacts, which contribute the highest risk group for leprosy development, is not comprehensive. Development of highly sensitive diagnostic methods to screen this population is need of the hour. Present study exploited the proteomics tools for differential expression of novel proteins in sera of contact and leprosy case as compared to healthy control. Highly abundant proteins were removed from the serum sample, followed by two-dimensional gel electrophoresis and liquid chromatography-mass spectrometry, bioinformatics tools for protein identification. On analyzing and comparing the two-dimensional patterns, we observed differential expression of five proteins, four proteins were over-expressed, and ...

ABSTRACT Turkey hens show spontaneous parthenogenesis (embryo development without any male contri... more ABSTRACT Turkey hens show spontaneous parthenogenesis (embryo development without any male contribution) which depends upon the genetic and environmental factors. A study was conducted to quantify the expression of genes (PouV, Sox2 and GATA-4) regulating parthenogenetic development in Beltsville Small White turkey. The parthenogenetic eggs from virgin turkey hens (30wk) and normal fertile eggs (by pen mating) were collected daily fumigated and set in incubator at 37.50C and 60% relative humidity. The eggs were broken at periodic intervals (0, 12, 24, 36 and 48hrs of incubation) and those showing positive development, their blastoderms/embryos were subjected to total RNA extraction by TRIZOL method. The concentration of each RNA sample was adjusted to a uniform concentration (2500 ng/µl) for first strand cDNA synthesis by Revert AidTM kit. PCR amplification was carried out in real time PCR (Bio-Rad, CFX Manager) and relative expression of target gene was calculated using Rest-2009 software. Expression pattern of 28s gene (house keeping) was used as reference for normalization of data. Expression of PouV was higher when the eggs were laid, but significantly (P<0.05) up-regulated at 12 and 24 hrs of incubation in parthenogenetic embryos than those normal fertilized embryos. Thereafter, the expression was down-regulated till 48 hrs of incubation. Expression of Sox2 in parthenogenetic embryos was higher till 12 hrs of incubation, and thereafter it declined and at 36 and 48 hrs expression was significantly (P<0.05) down-regulated. Expression of GATA-4 was not different in parthenogenetic and normal fertilized embryo up to 48hrs of incubation, however, apparently higher expression was seen in parthenogenetic embryos during 12-24 hrs of incubation. PouV, homologue to mammalian Oct-4 is required to maintain the pluripotency and self-renewal of embryonic stem cell. Before differentiation in embryonic stem cells, expression of Sox2 remains higher. From the present study, it was evident that there was delay in the developmental process in parthenogenetic turkey embryos. Key Words: mRNA expression, PouV, Sox2, Gata-4, parthenogenetic embryo, turkey.

The Veterinary quarterly, Jan 29, 2016

Paratuberculosis (pTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. ... more Paratuberculosis (pTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP) in a wide variety of domestic and wild animals. Control of pTB is difficult due to the lack of sensitive, efficacious and cost-effective diagnostics and marker vaccines. Microscopy, culture, and PCR have been used for the screening of MAP infection in animals for quite a long time. Besides, giving variable sensitivity and specificity, these tests have not been considered ideal for large-scale screening of domestic livestock. Serological tests like ELISA easily detects anti-MAP antibodies. However, it cannot differentiate between the vaccinated and infected animals. Nanotechnology-based diagnostic tests are underway to improve the sensitivity and specificity. Newer generation diagnostic tests based on recombinant MAP secretory proteins would open new paradigm for the differentiation between infected and vaccinated animals and for early detection of the infection. Du...

Indian Journal of Experimental Biology, Apr 1, 2010

Ocimum sanctum, a well known herb in Indian medicine, possesses various therapeutic properties in... more Ocimum sanctum, a well known herb in Indian medicine, possesses various therapeutic properties including healing properties and cytokine induction. Wound healing activity of cold aqueous extract of O. sanctum leaves along with its effect on tumor necrosis factor-alpha (TNF-alpha) was assessed using excision model of wound repair in Wistar albino rats. After application of the O. sanctum extract, rate of epithelization with an increase in wound contraction was observed. In animals, treated with 10% O. sanctum extract in petroleum jelly, wound healing was faster as compared to control group which were treated with petroleum jelly alone but significant accelerated healing was noticed in animals which in addition to the topical application of 10% extract of O. sanctum, were prefed with 250 mg/kg body weight of aqueous O. sanctum extract daily for 20 consecutive days. During wound healing phase TNF-alpha level was found to be up regulated by O. sanctum treatment. Early wound healing may be pronounced due to O. sanctum extract, by elevating TNF-alpha production.

International Journal of Applied Sciences and Biotechnology, 2016

Plants play a major role on the earth and human beings depend on plants because of their medicina... more Plants play a major role on the earth and human beings depend on plants because of their medicinal properties. About 80% of the world population using plants as a medicinal drugs because plants have no side effects and show synergistic effect unlike modern medicine. In the present study, review and authentication of the various aspects of the plant Lawsonia inermis was carried out. This plant is mainly present in subtropical and tropical areas and is used in all over the world. The common name of L. inermis is Henna and Mehndi. It has been used for over 9000 years for their cosmetic values as a dye. Traditionally, in Asian countries like India and Pakistan, plant leaves are applied to hands, hairs and feet. Morphologically the plant is a small tree or shrub. L. inermis is cultivated for roots, flowers, stem bark and seeds for their medicinal uses. Proteins, carbohydrates and fatty acid are primary metabolites along with secondary metabolite such as tannins, quinines, terpenoids, coumarins etc which are present in L. inermis plant. Phytochemical constituents of L. inermis are responsible for its analgesic, antiinflammatory, hepatoprotective and hypoglycaemic properties. The plant has also been reported for antibacterial, antifungal, immunostimulatory, antioxidant and cytotoxic activity. Presence of these properties in L. inermis plant develops it as medicine against various pathogenic organism and diseases. This review gives a wide view on the phytochemistry, pharmacological properties and traditional uses of the plant.

Indian Journal of Experimental Biology, Apr 1, 2010

Advances in Pharmaceutical and Ethnomedicines, 2015

Frontiers in Pharmacology, Dec 4, 2023

Journal of Young Pharmacists, Aug 24, 2023

Current Topics in Medicinal Chemistry

Reproduction in Domestic Animals, 2016

Small Ruminant Research, 2006

Journal of Ecophysiology and Occupational Health, Dec 14, 2023

Indian Journal of Small Ruminants (The)

Indian Journal of Animal Sciences, Sep 10, 2020

Materials Today: Proceedings

IOP Conference Series: Materials Science and Engineering, 2021

Journal of Infectious Diseases and Therapy, Sep 22, 2016

The Veterinary quarterly, Jan 29, 2016

Indian Journal of Experimental Biology, Apr 1, 2010

International Journal of Applied Sciences and Biotechnology, 2016

Indian Journal of Experimental Biology, Apr 1, 2010

Advances in Pharmaceutical and Ethnomedicines, 2015

Algae like some other higher plants, produces stored lipids in the form of triacylglycerol (TAG).... more Algae like some other higher plants, produces stored lipids in the form of triacylglycerol (TAG). Comparatively algae produce more oil than any other biofuel technology which is currently being used. Micro-algal species can accumulate substantial quantity of lipid, sometimes even 50% of their biomass. Algae can inhabitant a wide variety of natural habitats, from terrestrial habitats to aquatic ones. As 70% of our Earth is covered with water, so even if we can utilize even 1% of this water then also it will be sufficient to meet our energy need. Biofuels from algae may be produced from micro-algae or macro-algae i.e., Seaweeds, or Cyanobacteria. Cultivation of microalgae, cyanobacteria can be done through photoautotrophic or heterotrophic methods. Photoautotrophic method is the one in which the algae needs the light to grow and generate biomass while in the heterotrophic method, the algae are grown without light directly fed on carbon source. As algae possess higher photosynthetic efficiency than any other biomass so the CO2 sequestration can be performed much more effectively and easily. In addition to production of biofuel, algae can be used as a fertilizer, pollution controller, as well as food source, etc. For obtaining biofuel economically mass-cultivation of the algae should be carried out in either open pond system or in the case of enclosed photobioreactors. The open pond system is the oldest, simplest and easiest method to cultivate microalgae in large amount. These consist of a shallow pond which is about one feet deep. The greatest disadvantage of such type of system includes water loss due to evaporation, contamination with unwanted algal species as well as organisms that feed on algae. Enclosed photobioreactors system consist of a transparent material in which the algal cells are made to grow and are generally placed outdoor for illumination my natural light moreover the optimization of photobioreactors is a still difficult process to carry out. Algal Biofuels seems to be the best candidate which can replace the petroleum biodiesel in near future. Although, currently biodiesel production is still a costly affair due to high cost associated with oil extraction and biodiesel production. With the recent advances and breakthrough in biotechnology the new algal energy revolution is on the verge of creating potent energy source for future generation.

The evolution of life on Earth depends upon the organism’s ability to harness new and effective e... more The evolution of life on Earth depends upon the organism’s ability to harness new and effective energy sources to fuel metabolic processes. Since ancient times humans have explored nature to find new energy sources which led to the discovery of fossil fuels. These fossil fuels have served as a double edged weapon although quenching our thrust for energy but simultaneously adding large amount of greenhouse gases into our atmosphere, thus necessitating the search for alternate fuels.

Biofuels include any fuel obtained from living or dead organisms. Scientists & research workers have always searched for a low input high output, non palatable biofuel crop which should have the ability to grow rapidly in aquatic environment without competing with agricultural crops for space. The only one class of living microorganisms which comes close to possess all the above characteristic features is Algae. Thus the project is aimed to screen local water algal species of Mathura and adjoining region and predict their biofuel productivity under optimum conditions of growth and lipid productivity.