Ann-Helene Jakobsson - Academia.edu (original) (raw)

Papers by Ann-Helene Jakobsson

Research paper thumbnail of The semistability of centric chromatin bodies during long-term passage of multidrug resistant mouse-Chinese hamster cell hybrids

PubMed, May 1, 1988

In a cell fusion experiment with multidrug resistant (MDR) mouse SEWA tumor cells and sensitive C... more In a cell fusion experiment with multidrug resistant (MDR) mouse SEWA tumor cells and sensitive Chinese Hamster CHO cells, the resistant hybrid cells were completely without recognizable mouse chromosomes. Instead, numerous chromatin bodies (CB) were found that contained copies of a high molecular weight P-glycoprotein gene (PGY1) associated with the MDR condition. The present paper reports on the CB under long-term selective and nonselective growth. The CB were of a stability intermediate between that of double minutes (DM) and homogeneously staining regions (HSR). The stability of the CB was different in the two hybrid lines studied.

Research paper thumbnail of Fluorescentin Situ hybridization analysis of chromosomally normal gametes and abnormal arrested embryos

Journal of Assisted Reproduction and Genetics, Aug 1, 1995

ObjectiveThe purpose of the present study was to investigate if the arrested embryos from a coupl... more ObjectiveThe purpose of the present study was to investigate if the arrested embryos from a couple with several previously failed IVF treatments were chromosomally normal. Probes for chromosomes X, Y and 18 were used.ResultsA couple had undergone 7in vitro fertilization treatments over a 2 1/2-year period without achieving a pregnancy. In each cycle, where fertilization was obtained, the development of the embryos was arrested. Fluorescentin situ hybridization probes for chromosomes X and Y (and 18) was carried out on gametes and on embryos in 2 separate cycles. Sperm and oocytes were normally haploid X0 or Y0. The nuclei of the blastomeres were fragmented and mosaic for X or Y, or monosomic X0, despite the fact that 2 pronuclei had been assessed on day 1 following intracytoplasmic sperm injection.ConclusionChromosomally normal gametes can result in abnormal embryos manifested by arrested development and unexplained infertility.

Research paper thumbnail of The outcome of intracytoplasmic sperm injection is unrelated to 'strict criteria' sperm morphology

Human Reproduction, May 1, 1996

Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertili... more Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertilization treatment in the semen samples used for 354 consecutive treatment cycles for intracytoplasmic sperm injection (ICSI). The semen samples were classified according to the three predictive categories of the Tygerberg strict criteria: excellent prognosis (>14% morphologically normal spermatozoa), good prognosis (4-14%) and poor prognosis (<4%). It was found that 37 (10.5%) of the ICSI cycles belonged to the excellent prognosis category, 197 (55.6%) to the good prognosis category, and 120 (33.9%) to the poor prognosis category. The outcomes of the ICSI treatments were evaluated and compared with the sperm morphology classification in order to determine whether the strict criteria could aid in predicting the outcome of ICSI. The fertilization rates in the three categories were 61.6, 66.8, and 61.9%, the pregnancy rates per oocyte retrieval 18.9, 24.9, and 283%, and the implantation rates 9.9, 13.0, and 14.9% respectively. No significant differences were found in fertilization, pregnancy, or implantation rates between the three prognosis categories, i.e. the poor prognosis category had an equal chance of obtaining pregnancy compared with the good prognosis category. The results indicate that strict sperm morphology is not related to the outcome of ICSI.

Research paper thumbnail of 5 Persistence of fragmented mouse HSR chromatin in mouse-chinese hamster somatic cell hybrids resistant to actinomycin D

Cancer genetics and cytogenetics, Sep 1, 1987

Research paper thumbnail of Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility

Fertility and Sterility, Apr 1, 1995

Objective: To establish an intracytoplasmic sperm injection treatment program for couples with ma... more Objective: To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. Design: A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. Setting: Infertility clinic in a private hospital associated with a university hospital. Patients: Couples with infertility in the male partner whose sperm parameters were unacceptable for conventional IVF or in whom fertilization by conventional IVF failed repeatedly. Interventions: One hundred seventy-one transvaginal oocyte retrievals were completed after superovulation with GnRH agonist and gonadotropins. Main Outcome Measures: The parameters evaluated included fertilization, cleavage, implantation, pregnancy, and spontaneous abortion in relation to patient indications and improved procedures. Results: After intracytoplasmic sperm injection, normal fertilization occurred in 45% of the oocytes (n = 1,499). Of 171 treatment cycles, 93% of the couples had fertilization and 86% had ET. Thirty-six pregnancies were achieved. During the period studied, the mean fertilization rate increased from 21.3% during the first 17 weeks to 67.8% during the last 13 weeks, and the pregnancy rate (PR) per started cycle increased from 12.8% to 31.3%. Conclusions: Technical factors critical for achieving high rates of fertilization and pregnancy were the use of standardized intracytoplasmic sperm injection pipettes, the immobilization of sperm before injection, and the aspiration of a minimal amount of ooplasm before reinjection with the sperm. Intracytoplasmic sperm injection appears to be superior to other micromanipulation methods for alleviating male infertility.

Research paper thumbnail of Cytogenetic and molecular analysis of drug resistance in interspecific cell hybrids

Research paper thumbnail of The concentration of lidocaine in follicular fluid when used for paracervical block in a human IVF-ET programme

Human Reproduction, 1990

Lidocaine is a well-documented local anaesthetic often used for paracervical block (PCB) in pregn... more Lidocaine is a well-documented local anaesthetic often used for paracervical block (PCB) in pregnant women. For this reason, the substance was used early on for PCB in connection with transvaginal follicle aspiration. However, the concentration reached in follicular fluid after PCB or local infiltration of the vaginal wall has not been previously determined. Furthermore, if lidocaine reaches the follicle, it seems important to determine if the concentration reached influences fertilization and early cleavage of the embryo and the pregnancy rate. The study included 46 women who had PCB with lidocaine (50 mg) and 46 women who had no PCB in connection with follicle aspiration. The mean concentration in follicular fluid was 0.36 +/- 1.1 micrograms/ml. There was no significant difference in lidocaine concentration between follicles containing oocytes that were fertilized and those that were not. Furthermore, the fertilization and cleavage rates did not differ significantly in women with and without PCB with lidocaine. The pregnancy rate did not differ between the two groups. It thus seems that the concentration of lidocaine found in the follicular fluid after PCB with 50 mg lidocaine does not negatively affect fertilization of the human oocyte or early cleavage of the human embryo.

Research paper thumbnail of Unrelated to &Apos;Strict Criteria &Apos; Sperm Morphology

Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertili... more Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertilization treatment in the semen samples used for 354 consecutive treatment cycles for intracytoplasmic sperm injection (ICSI). The semen samples were classified according to the three predictive categories of the Tygerberg strict criteria: excellent prognosis (>14% morphologically normal spermatozoa), good prognosis (4-14%) and poor prognosis (<4%). It was found that 37 (10.5%) of the ICSI cycles belonged to the excellent prognosis category, 197 (55.6%) to the good prognosis category, and 120 (33.9%) to the poor prognosis category. The outcomes of the ICSI treatments were evaluated and compared with the sperm morphology classification in order to determine whether the strict criteria could aid in predicting the outcome of ICSI. The fertilization rates in the three categories were 61.6, 66.8, and 61.9%, the pregnancy rates per oocyte retrieval 18.9, 24.9, and 283%, and the implantation rates 9.9, 13.0, and 14.9% respectively. No significant differences were found in fertilization, pregnancy, or implantation rates between the three prognosis categories, i.e. the poor prognosis category had an equal chance of obtaining pregnancy compared with the good prognosis category. The results indicate that strict sperm morphology is not related to the outcome of ICSI.

Research paper thumbnail of Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility

International Journal of Gynecology & Obstetrics, 1996

To establish an intracytoplasmic sperm injection treatment program for couples with male infertil... more To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. Infertility clinic in a private hospital associated with a university hospital. Couples with infertility in the male partner whose sperm parameters were unacceptable for conventional IVF or in whom fertilization by conventional IVF failed repeatedly. One hundred seventy-one transvaginal oocyte retrievals were completed after superovulation with GnRH agonist and gonadotropins. The parameters evaluated included fertilization, cleavage, implantation, pregnancy, and spontaneous abortion in relation to patient indications and improved procedures. After intracytoplasmic sperm injection, normal fertilization occurred in 45% of the oocytes (n = 1,499). Of 171 treatment cycles, 93% of the couples had fertilization and 86% had ET. Thirty-six pregnancies were achieved. During the period studied, the mean fertilization rate increased from 21.3% during the first 17 weeks to 67.8% during the last 13 weeks, and the pregnancy rate (PR) per started cycle increased from 12.8% to 31.3%. Technical factors critical for achieving high rates of fertilization and pregnancy were the use of standardized intracytoplasmic sperm injection pipettes, the immobilization of sperm before injection, and the aspiration of a minimal amount of ooplasm before reinjection with the sperm. Intracytoplasmic sperm injection appears to be superior to other micromanipulation methods for alleviating male infertility.

Research paper thumbnail of Subzonal insemination (SUZI) or in vitro fertilization (IVF) in microdroplets for the treatment of male-factor infertility

Journal of Assisted Reproduction and Genetics, 1994

The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet insemina... more The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet insemination used in couples with male-factor infertility are presented. The total fertilization rate was 17.4% for SUZI (n = 89) and 49.3% for microdroplet IVF (n = 100). The fertilization rate for standard IVF (n = 510), not including any male-factor infertility and performed during the same period, was 73.2%. The &amp;amp;amp;amp;quot;take-home baby rate&amp;amp;amp;amp;quot; per started cycle and per embryo transfer (ET), respectively, was 10 and 17.6% for SUZI and 20 and 24.7% for microdrop IVF. For standard IVF these figures were 27 and 31.7%. It was concluded that microdroplet IVF can be used with good results in cases of moderate male-factor infertility. The normal (2PN) fertilization rate with the SUZI technique was only 15.1%. However, despite the low fertilization rate, SUZI should be considered when dealing with severe male-factor infertility.

Research paper thumbnail of Novel cytogenetic expression of gene amplification in actinomycin D-resistant somatic cell hybrids: transfer of resistance by centric chromatin bodies

Chromosoma, 1987

SEWATC13 mouse cells, resistant to 0.1 microgram/ml of actinomycin D (AMD), were fused to AMD-sen... more SEWATC13 mouse cells, resistant to 0.1 microgram/ml of actinomycin D (AMD), were fused to AMD-sensitive cells of the Chinese hamster ovary cell line (CHO). Twenty-two hybrid clones were isolated and put into serial culture in the selective medium. Unexpectedly, identifiable mouse chromosomes were found only in one of the hybrids. All the others had only hamster chromosomes and, in addition, numerous chromatin bodies (CBs), mostly small and irregularly shaped, but also larger, more chromosome-like ones. The CBs were distinctly C-band positive and a mouse satellite probe hybridized strongly to them. The AMD resistance of the murine parental cells had previously been attributed to gene amplification in two large homogeneously staining regions (HSR-AMD1 and 2). They were not observed in the hybrid cells but had supposedly reappeared in the guise of the CBs. It was established by Southern DNA blot analysis that amplified DNA sequences, localized to the HSR-AMD1 and 2 of the SEWA parent were present in multiple copies in the hybrids. It was also established by in situ hybridization that they were located in the CBs. Unlike double minutes (DMs) the CBs were all centric.

Research paper thumbnail of Preimplantation genetic diagnosis (PGD): The Gothenburg experience

Acta Obstetricia et Gynecologica Scandinavica, 2001

A program for preimplantation genetic diagnosis of pre-embryos from patients with hereditary diso... more A program for preimplantation genetic diagnosis of pre-embryos from patients with hereditary disorders was set up in our unit at Sahlgrenska University Hospital in 1994. The majority of the patients were carriers of X-chromosome linked disorders; a few patients were translocation carriers. In this paper we describe our experiences of our first 36 cycles, 30 gender determinations and six analyses of embryos with possible translocations. Conventional hormone replacement treatment with intracytoplasmic sperm injection to fertilize the eggs followed by blastomere biopsy and fluorescent in situ hybridization at the eight cell stage was used for sexing as well as detection of translocations. Out of the 30 cycles in 13 patients for gender determination, blastomere biopsies could be carried out in 25 cycles. Transfer of normal female embryos (XX) was performed in 18 cycles, resulting in five pregnancies (pregnancy rate 27.8%) and an implantation rate of 20% per transfer. Three girls have been born. Hence the take home baby rate was 16.7% per transfer and 10% per started cycle. Six cycles (three patients) for detection of translocations in embryos were performed. Diagnosis was possible in four cycles. Transfer of normal embryos was carried out in one cycle. No pregnancy was achieved. Successful PGD in its clinical application demands close collaboration between a large group of specialists. Even so, the success rate is considerably lower than after conventional IVF or ICSI procedures. Taking into account the stress caused to the parents facing late interruption of pregnancy following conventional prenatal diagnosis we are convinced that this technique is well worthwhile continuing and refining.

Research paper thumbnail of Transfer of methotrexate resistance by somatic cell hybridization

Hereditas, 2008

... ANN-HELENE JAKOBSSON, BJORN DAHLLOF, TOMMY MARTINSSON and GORAN LEVAN ... diluted 15-and 3-fo... more ... ANN-HELENE JAKOBSSON, BJORN DAHLLOF, TOMMY MARTINSSON and GORAN LEVAN ... diluted 15-and 3-fold with water and the amount of protein was determined by measuring the optical density at 235 and 280 nm according to the method of WHITAKER and GRANUM ...

Research paper thumbnail of Subzonal Insemination (IVF) in Microdroplets Male-Factor Infertility (SUZI) for the or in Vitro Fertilization Treatment of

Journal of Assisted Reproduction and Genetics, 1994

Purpose: The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet... more Purpose: The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet insemination used in couples with male-factor infertility are presented. Results: The total fertilization rate was 17.4% for SUZI (n =89) and 49.3% for microdroplet IVF (n = 100). The fertilization rate for standard IVF (n = 510), not including any male-factor infertility and performed during the same period, was 73.2%. The "take-home baby rate" per started cycle and per embryo transfer (ET), respectively, was 10 and 17.6%for SUZI and 20 and 24.7% for microdrop IVF. For standard IVF these figures were 27 and 31.7%. Conclusion: It was concluded that microdroplet IVF can be used with good results in cases of moderate malefactor infertility. The normal (2PN) fertilization rate with the SUZI technique was only 15.1%. However, despite the low fertilization rate, SUZ! should be considered when dealing with severe male-factor infertility.

Research paper thumbnail of P-078. Simplified recovery of spermatozoa from men with obstructive and non-obstructive azoospermia

Human Reproduction, Jun 1, 1997

Research paper thumbnail of The semistability of centric chromatin bodies during long-term passage of multidrug resistant mouse-Chinese hamster cell hybrids

PubMed, May 1, 1988

In a cell fusion experiment with multidrug resistant (MDR) mouse SEWA tumor cells and sensitive C... more In a cell fusion experiment with multidrug resistant (MDR) mouse SEWA tumor cells and sensitive Chinese Hamster CHO cells, the resistant hybrid cells were completely without recognizable mouse chromosomes. Instead, numerous chromatin bodies (CB) were found that contained copies of a high molecular weight P-glycoprotein gene (PGY1) associated with the MDR condition. The present paper reports on the CB under long-term selective and nonselective growth. The CB were of a stability intermediate between that of double minutes (DM) and homogeneously staining regions (HSR). The stability of the CB was different in the two hybrid lines studied.

Research paper thumbnail of Fluorescentin Situ hybridization analysis of chromosomally normal gametes and abnormal arrested embryos

Journal of Assisted Reproduction and Genetics, Aug 1, 1995

ObjectiveThe purpose of the present study was to investigate if the arrested embryos from a coupl... more ObjectiveThe purpose of the present study was to investigate if the arrested embryos from a couple with several previously failed IVF treatments were chromosomally normal. Probes for chromosomes X, Y and 18 were used.ResultsA couple had undergone 7in vitro fertilization treatments over a 2 1/2-year period without achieving a pregnancy. In each cycle, where fertilization was obtained, the development of the embryos was arrested. Fluorescentin situ hybridization probes for chromosomes X and Y (and 18) was carried out on gametes and on embryos in 2 separate cycles. Sperm and oocytes were normally haploid X0 or Y0. The nuclei of the blastomeres were fragmented and mosaic for X or Y, or monosomic X0, despite the fact that 2 pronuclei had been assessed on day 1 following intracytoplasmic sperm injection.ConclusionChromosomally normal gametes can result in abnormal embryos manifested by arrested development and unexplained infertility.

Research paper thumbnail of The outcome of intracytoplasmic sperm injection is unrelated to 'strict criteria' sperm morphology

Human Reproduction, May 1, 1996

Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertili... more Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertilization treatment in the semen samples used for 354 consecutive treatment cycles for intracytoplasmic sperm injection (ICSI). The semen samples were classified according to the three predictive categories of the Tygerberg strict criteria: excellent prognosis (>14% morphologically normal spermatozoa), good prognosis (4-14%) and poor prognosis (<4%). It was found that 37 (10.5%) of the ICSI cycles belonged to the excellent prognosis category, 197 (55.6%) to the good prognosis category, and 120 (33.9%) to the poor prognosis category. The outcomes of the ICSI treatments were evaluated and compared with the sperm morphology classification in order to determine whether the strict criteria could aid in predicting the outcome of ICSI. The fertilization rates in the three categories were 61.6, 66.8, and 61.9%, the pregnancy rates per oocyte retrieval 18.9, 24.9, and 283%, and the implantation rates 9.9, 13.0, and 14.9% respectively. No significant differences were found in fertilization, pregnancy, or implantation rates between the three prognosis categories, i.e. the poor prognosis category had an equal chance of obtaining pregnancy compared with the good prognosis category. The results indicate that strict sperm morphology is not related to the outcome of ICSI.

Research paper thumbnail of 5 Persistence of fragmented mouse HSR chromatin in mouse-chinese hamster somatic cell hybrids resistant to actinomycin D

Cancer genetics and cytogenetics, Sep 1, 1987

Research paper thumbnail of Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility

Fertility and Sterility, Apr 1, 1995

Objective: To establish an intracytoplasmic sperm injection treatment program for couples with ma... more Objective: To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. Design: A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. Setting: Infertility clinic in a private hospital associated with a university hospital. Patients: Couples with infertility in the male partner whose sperm parameters were unacceptable for conventional IVF or in whom fertilization by conventional IVF failed repeatedly. Interventions: One hundred seventy-one transvaginal oocyte retrievals were completed after superovulation with GnRH agonist and gonadotropins. Main Outcome Measures: The parameters evaluated included fertilization, cleavage, implantation, pregnancy, and spontaneous abortion in relation to patient indications and improved procedures. Results: After intracytoplasmic sperm injection, normal fertilization occurred in 45% of the oocytes (n = 1,499). Of 171 treatment cycles, 93% of the couples had fertilization and 86% had ET. Thirty-six pregnancies were achieved. During the period studied, the mean fertilization rate increased from 21.3% during the first 17 weeks to 67.8% during the last 13 weeks, and the pregnancy rate (PR) per started cycle increased from 12.8% to 31.3%. Conclusions: Technical factors critical for achieving high rates of fertilization and pregnancy were the use of standardized intracytoplasmic sperm injection pipettes, the immobilization of sperm before injection, and the aspiration of a minimal amount of ooplasm before reinjection with the sperm. Intracytoplasmic sperm injection appears to be superior to other micromanipulation methods for alleviating male infertility.

Research paper thumbnail of Cytogenetic and molecular analysis of drug resistance in interspecific cell hybrids

Research paper thumbnail of The concentration of lidocaine in follicular fluid when used for paracervical block in a human IVF-ET programme

Human Reproduction, 1990

Lidocaine is a well-documented local anaesthetic often used for paracervical block (PCB) in pregn... more Lidocaine is a well-documented local anaesthetic often used for paracervical block (PCB) in pregnant women. For this reason, the substance was used early on for PCB in connection with transvaginal follicle aspiration. However, the concentration reached in follicular fluid after PCB or local infiltration of the vaginal wall has not been previously determined. Furthermore, if lidocaine reaches the follicle, it seems important to determine if the concentration reached influences fertilization and early cleavage of the embryo and the pregnancy rate. The study included 46 women who had PCB with lidocaine (50 mg) and 46 women who had no PCB in connection with follicle aspiration. The mean concentration in follicular fluid was 0.36 +/- 1.1 micrograms/ml. There was no significant difference in lidocaine concentration between follicles containing oocytes that were fertilized and those that were not. Furthermore, the fertilization and cleavage rates did not differ significantly in women with and without PCB with lidocaine. The pregnancy rate did not differ between the two groups. It thus seems that the concentration of lidocaine found in the follicular fluid after PCB with 50 mg lidocaine does not negatively affect fertilization of the human oocyte or early cleavage of the human embryo.

Research paper thumbnail of Unrelated to &Apos;Strict Criteria &Apos; Sperm Morphology

Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertili... more Sperm morphology was assessed according to the 'strict criteria' established for in-vitro fertilization treatment in the semen samples used for 354 consecutive treatment cycles for intracytoplasmic sperm injection (ICSI). The semen samples were classified according to the three predictive categories of the Tygerberg strict criteria: excellent prognosis (>14% morphologically normal spermatozoa), good prognosis (4-14%) and poor prognosis (<4%). It was found that 37 (10.5%) of the ICSI cycles belonged to the excellent prognosis category, 197 (55.6%) to the good prognosis category, and 120 (33.9%) to the poor prognosis category. The outcomes of the ICSI treatments were evaluated and compared with the sperm morphology classification in order to determine whether the strict criteria could aid in predicting the outcome of ICSI. The fertilization rates in the three categories were 61.6, 66.8, and 61.9%, the pregnancy rates per oocyte retrieval 18.9, 24.9, and 283%, and the implantation rates 9.9, 13.0, and 14.9% respectively. No significant differences were found in fertilization, pregnancy, or implantation rates between the three prognosis categories, i.e. the poor prognosis category had an equal chance of obtaining pregnancy compared with the good prognosis category. The results indicate that strict sperm morphology is not related to the outcome of ICSI.

Research paper thumbnail of Factors of importance for the establishment of a successful program of intracytoplasmic sperm injection treatment for male infertility

International Journal of Gynecology & Obstetrics, 1996

To establish an intracytoplasmic sperm injection treatment program for couples with male infertil... more To establish an intracytoplasmic sperm injection treatment program for couples with male infertility and to determine those factors important for success. A retrospective analysis of 171 consecutive cycles of intracytoplasmic sperm injection concerning 145 infertile couples. Infertility clinic in a private hospital associated with a university hospital. Couples with infertility in the male partner whose sperm parameters were unacceptable for conventional IVF or in whom fertilization by conventional IVF failed repeatedly. One hundred seventy-one transvaginal oocyte retrievals were completed after superovulation with GnRH agonist and gonadotropins. The parameters evaluated included fertilization, cleavage, implantation, pregnancy, and spontaneous abortion in relation to patient indications and improved procedures. After intracytoplasmic sperm injection, normal fertilization occurred in 45% of the oocytes (n = 1,499). Of 171 treatment cycles, 93% of the couples had fertilization and 86% had ET. Thirty-six pregnancies were achieved. During the period studied, the mean fertilization rate increased from 21.3% during the first 17 weeks to 67.8% during the last 13 weeks, and the pregnancy rate (PR) per started cycle increased from 12.8% to 31.3%. Technical factors critical for achieving high rates of fertilization and pregnancy were the use of standardized intracytoplasmic sperm injection pipettes, the immobilization of sperm before injection, and the aspiration of a minimal amount of ooplasm before reinjection with the sperm. Intracytoplasmic sperm injection appears to be superior to other micromanipulation methods for alleviating male infertility.

Research paper thumbnail of Subzonal insemination (SUZI) or in vitro fertilization (IVF) in microdroplets for the treatment of male-factor infertility

Journal of Assisted Reproduction and Genetics, 1994

The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet insemina... more The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet insemination used in couples with male-factor infertility are presented. The total fertilization rate was 17.4% for SUZI (n = 89) and 49.3% for microdroplet IVF (n = 100). The fertilization rate for standard IVF (n = 510), not including any male-factor infertility and performed during the same period, was 73.2%. The &amp;amp;amp;amp;quot;take-home baby rate&amp;amp;amp;amp;quot; per started cycle and per embryo transfer (ET), respectively, was 10 and 17.6% for SUZI and 20 and 24.7% for microdrop IVF. For standard IVF these figures were 27 and 31.7%. It was concluded that microdroplet IVF can be used with good results in cases of moderate male-factor infertility. The normal (2PN) fertilization rate with the SUZI technique was only 15.1%. However, despite the low fertilization rate, SUZI should be considered when dealing with severe male-factor infertility.

Research paper thumbnail of Novel cytogenetic expression of gene amplification in actinomycin D-resistant somatic cell hybrids: transfer of resistance by centric chromatin bodies

Chromosoma, 1987

SEWATC13 mouse cells, resistant to 0.1 microgram/ml of actinomycin D (AMD), were fused to AMD-sen... more SEWATC13 mouse cells, resistant to 0.1 microgram/ml of actinomycin D (AMD), were fused to AMD-sensitive cells of the Chinese hamster ovary cell line (CHO). Twenty-two hybrid clones were isolated and put into serial culture in the selective medium. Unexpectedly, identifiable mouse chromosomes were found only in one of the hybrids. All the others had only hamster chromosomes and, in addition, numerous chromatin bodies (CBs), mostly small and irregularly shaped, but also larger, more chromosome-like ones. The CBs were distinctly C-band positive and a mouse satellite probe hybridized strongly to them. The AMD resistance of the murine parental cells had previously been attributed to gene amplification in two large homogeneously staining regions (HSR-AMD1 and 2). They were not observed in the hybrid cells but had supposedly reappeared in the guise of the CBs. It was established by Southern DNA blot analysis that amplified DNA sequences, localized to the HSR-AMD1 and 2 of the SEWA parent were present in multiple copies in the hybrids. It was also established by in situ hybridization that they were located in the CBs. Unlike double minutes (DMs) the CBs were all centric.

Research paper thumbnail of Preimplantation genetic diagnosis (PGD): The Gothenburg experience

Acta Obstetricia et Gynecologica Scandinavica, 2001

A program for preimplantation genetic diagnosis of pre-embryos from patients with hereditary diso... more A program for preimplantation genetic diagnosis of pre-embryos from patients with hereditary disorders was set up in our unit at Sahlgrenska University Hospital in 1994. The majority of the patients were carriers of X-chromosome linked disorders; a few patients were translocation carriers. In this paper we describe our experiences of our first 36 cycles, 30 gender determinations and six analyses of embryos with possible translocations. Conventional hormone replacement treatment with intracytoplasmic sperm injection to fertilize the eggs followed by blastomere biopsy and fluorescent in situ hybridization at the eight cell stage was used for sexing as well as detection of translocations. Out of the 30 cycles in 13 patients for gender determination, blastomere biopsies could be carried out in 25 cycles. Transfer of normal female embryos (XX) was performed in 18 cycles, resulting in five pregnancies (pregnancy rate 27.8%) and an implantation rate of 20% per transfer. Three girls have been born. Hence the take home baby rate was 16.7% per transfer and 10% per started cycle. Six cycles (three patients) for detection of translocations in embryos were performed. Diagnosis was possible in four cycles. Transfer of normal embryos was carried out in one cycle. No pregnancy was achieved. Successful PGD in its clinical application demands close collaboration between a large group of specialists. Even so, the success rate is considerably lower than after conventional IVF or ICSI procedures. Taking into account the stress caused to the parents facing late interruption of pregnancy following conventional prenatal diagnosis we are convinced that this technique is well worthwhile continuing and refining.

Research paper thumbnail of Transfer of methotrexate resistance by somatic cell hybridization

Hereditas, 2008

... ANN-HELENE JAKOBSSON, BJORN DAHLLOF, TOMMY MARTINSSON and GORAN LEVAN ... diluted 15-and 3-fo... more ... ANN-HELENE JAKOBSSON, BJORN DAHLLOF, TOMMY MARTINSSON and GORAN LEVAN ... diluted 15-and 3-fold with water and the amount of protein was determined by measuring the optical density at 235 and 280 nm according to the method of WHITAKER and GRANUM ...

Research paper thumbnail of Subzonal Insemination (IVF) in Microdroplets Male-Factor Infertility (SUZI) for the or in Vitro Fertilization Treatment of

Journal of Assisted Reproduction and Genetics, 1994

Purpose: The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet... more Purpose: The results of subzonal insemination (SUZI) and in vitro fertilization with microdroplet insemination used in couples with male-factor infertility are presented. Results: The total fertilization rate was 17.4% for SUZI (n =89) and 49.3% for microdroplet IVF (n = 100). The fertilization rate for standard IVF (n = 510), not including any male-factor infertility and performed during the same period, was 73.2%. The "take-home baby rate" per started cycle and per embryo transfer (ET), respectively, was 10 and 17.6%for SUZI and 20 and 24.7% for microdrop IVF. For standard IVF these figures were 27 and 31.7%. Conclusion: It was concluded that microdroplet IVF can be used with good results in cases of moderate malefactor infertility. The normal (2PN) fertilization rate with the SUZI technique was only 15.1%. However, despite the low fertilization rate, SUZ! should be considered when dealing with severe male-factor infertility.

Research paper thumbnail of P-078. Simplified recovery of spermatozoa from men with obstructive and non-obstructive azoospermia

Human Reproduction, Jun 1, 1997