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Papers by Anna Boero
Cell Biochem Biophys, 1987
A pigment made up of a protein able to bind retinal as well as retinol is described. The molecule... more A pigment made up of a protein able to bind retinal as well as retinol is described. The molecule consists of a dimer with a molecular weight of 50,000 which binds one molecule of retinal. The binding site for retinal is a Schiff base buried in the interior of the protein. Retinol is probably bound to the protein in the same site as for retinal, although not covalently, as suggested by the absorbance spectra. The protein, extracted from honeybee retina, is involved in visual pigment metabolism, and its structure may elucidate the mechanism of the stereospecific photoisomerization of all trans-retinal to 11-cis-retinal.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
The rod outer segments of toad retina contain a guanylate cyclase activity of about 3 + 1 nmol of... more The rod outer segments of toad retina contain a guanylate cyclase activity of about 3 + 1 nmol of cGMP formed/min per mg protein. In darkness this value is largely independent of the Ca 2+ concentration, although it is enhanced by light upon lowering the Ca 2+ concentration from 10-s to 10-8 M. The activating effect of light on cyclase at low Ca 2+ concentrations is enlarged upon increasing the light intensity. With a flash of light bleaching 7 • 10 -2 percent of rhodopsin, cyclase activity increased by a factor of 30 when Ca 2+ levels dropped from 10-s to 10-8 M. In view of recent observations that shortly after a flash of light the calcium activity inside the photoreceptor cell decreases, it seems likely that Ca 2 ÷ plays a regulatory role on cGMP metabolism in visual excitation.
Vaccine, 2005
The guinea pig model of recurrent genital herpes simplex virus type 2 (HSV-2) infection was used ... more The guinea pig model of recurrent genital herpes simplex virus type 2 (HSV-2) infection was used to test the immunotherapeutic activity of a glycoprotein subunit vaccine. Vaccine formulation consisted of three recombinant herpes simplex virus (HSV) glycoproteins, namely gB1s, gD2t and gE1t, plus aluminium hydroxide [Al(OH) 3 )] adjuvant. One month after viral challenge, infected animals were therapeutically immunised by seven subcutaneous injections of a low dose of antigens with a weekly interval for the first five and a fortnightly interval for the last two administrations. Results showed that the treatment was highly effective in ameliorating the recidivist pathology of animals, suggesting that this kind of vaccine formulation and administration may be helpful for therapeutic intervention in humans affected by recurrent herpes infections. 14 15 16 17 18 19 20 (R. Manservigi).
Cell Biochem Biophys, 1987
A pigment made up of a protein able to bind retinal as well as retinol is described. The molecule... more A pigment made up of a protein able to bind retinal as well as retinol is described. The molecule consists of a dimer with a molecular weight of 50,000 which binds one molecule of retinal. The binding site for retinal is a Schiff base buried in the interior of the protein. Retinol is probably bound to the protein in the same site as for retinal, although not covalently, as suggested by the absorbance spectra. The protein, extracted from honeybee retina, is involved in visual pigment metabolism, and its structure may elucidate the mechanism of the stereospecific photoisomerization of all trans-retinal to 11-cis-retinal.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
The rod outer segments of toad retina contain a guanylate cyclase activity of about 3 + 1 nmol of... more The rod outer segments of toad retina contain a guanylate cyclase activity of about 3 + 1 nmol of cGMP formed/min per mg protein. In darkness this value is largely independent of the Ca 2+ concentration, although it is enhanced by light upon lowering the Ca 2+ concentration from 10-s to 10-8 M. The activating effect of light on cyclase at low Ca 2+ concentrations is enlarged upon increasing the light intensity. With a flash of light bleaching 7 • 10 -2 percent of rhodopsin, cyclase activity increased by a factor of 30 when Ca 2+ levels dropped from 10-s to 10-8 M. In view of recent observations that shortly after a flash of light the calcium activity inside the photoreceptor cell decreases, it seems likely that Ca 2 ÷ plays a regulatory role on cGMP metabolism in visual excitation.
Vaccine, 2005
The guinea pig model of recurrent genital herpes simplex virus type 2 (HSV-2) infection was used ... more The guinea pig model of recurrent genital herpes simplex virus type 2 (HSV-2) infection was used to test the immunotherapeutic activity of a glycoprotein subunit vaccine. Vaccine formulation consisted of three recombinant herpes simplex virus (HSV) glycoproteins, namely gB1s, gD2t and gE1t, plus aluminium hydroxide [Al(OH) 3 )] adjuvant. One month after viral challenge, infected animals were therapeutically immunised by seven subcutaneous injections of a low dose of antigens with a weekly interval for the first five and a fortnightly interval for the last two administrations. Results showed that the treatment was highly effective in ameliorating the recidivist pathology of animals, suggesting that this kind of vaccine formulation and administration may be helpful for therapeutic intervention in humans affected by recurrent herpes infections. 14 15 16 17 18 19 20 (R. Manservigi).