Anna Mietelska-porowska - Academia.edu (original) (raw)

Papers by Anna Mietelska-porowska

Research paper thumbnail of Modulation of LPS-Induced Neurodegeneration by Intestinal Helminth Infection in Ageing Mice

International Journal of Molecular Sciences, Sep 11, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Greater sensitivity of the hippocampus than the entorhinal cortex in response to western diet, manifested by accelerated neuroinflammation and neurodegeneration in the progression of Alzheimer’s disease

Alzheimer's & Dementia, Nov 30, 2023

Research paper thumbnail of P4-003: Treatment with Lithium Chloride Alleviates Morphological and Cognitive Impairment Provoked by Excessive Tau in Pharmacologically Induced Rat Model of Human Tauopathy

Alzheimers & Dementia, Jul 1, 2014

WEDNESDAY, JULY 16, 2014 POSTER PRESENTATIONS P4 P4-001 CHANGES IN FRONTOCORTICAL INTERSTITIAL FL... more WEDNESDAY, JULY 16, 2014 POSTER PRESENTATIONS P4 P4-001 CHANGES IN FRONTOCORTICAL INTERSTITIAL FLUID LEVELS OF BETA-AMYLOID AND TAU PROTEIN IN TRANSGENIC MICE MODELS OF ALZHEIMER’S DISEASE Alain Gobert, Gaelle Rollin-Jego, Fabrice Iop, Jean Michel Rivet, Dominique Favale, Laurence Danober, Sophie Dix, Christian Czech, Ozmen Laurence, Jill Richardson, Esther Schenker, Institut de Recherches Servier, Croissy sur Seine, France; Institut de Recherches Servier, Croissy sur Seine, France; Institut de Recherches Servier, Croissy sur Seine, France; Eli Lilly, Lilly Research Centre, Surrey, United Kingdom; CNS Research, Hoffmann-La Roche AG, Basel, Switzerland; F. Hoffmann-LaRoche AG, Basel, Switzerland; GlaxoSmithKline Research and Development, China Group, Stevenage, United Kingdom; Institut de Recherches Servier, Croissy sur Seine, France. Contact e-mail: alain.gobert@fr.netgrs.com

Research paper thumbnail of Brain insulin resistance and Alzheimer’s molecular changes induced by Western diet

Alzheimer's & Dementia

BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simp... more BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simple sugars and saturated fats. Long‐term consumption of WD may lead to disruption of insulin signaling and development of insulin resistance. It is supposed that insulin resistance is a probable risk factors for Alzheimer’s disease (AD). The aim of this study was to verify this hypothesis in wild type mice by checking the WD effect on initiation and propagation of main neuropathological AD features such as amyloid‐β (Aβ) plaques and neurofibrillary tangles, which start from the entorhinal cortex in the temporal area, and progresses to the hippocampus, resulting in the loss of memory and cognition.MethodMales of wild type C57BL/6J mice were fed WD diet or standard diet (SD; CTR). Mice experimental groups (WD and CTR) were divided into age subgroups 4‐, 8‐, 12‐ and 16‐month‐old. In the first step, the WD‐diet dependent insulin signaling was analyzed, and the levels of the insulin pathway co...

Research paper thumbnail of Brain insulin resistance and Alzheimer’s molecular changes induced by Western diet

Alzheimer's & Dementia

BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simp... more BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simple sugars and saturated fats. Long‐term consumption of WD may lead to disruption of insulin signaling and development of insulin resistance. It is supposed that insulin resistance is a probable risk factors for Alzheimer’s disease (AD). The aim of this study was to verify this hypothesis in wild type mice by checking the WD effect on initiation and propagation of main neuropathological AD features such as amyloid‐β (Aβ) plaques and neurofibrillary tangles, which start from the entorhinal cortex in the temporal area, and progresses to the hippocampus, resulting in the loss of memory and cognition.MethodMales of wild type C57BL/6J mice were fed WD diet or standard diet (SD; CTR). Mice experimental groups (WD and CTR) were divided into age subgroups 4‐, 8‐, 12‐ and 16‐month‐old. In the first step, the WD‐diet dependent insulin signaling was analyzed, and the levels of the insulin pathway co...

Research paper thumbnail of Long-term effects of subdural injections of an antlerogenic cell homogenate (ACH) in a porcine model of spinal cord injury (SCI)

Acta Neurobiologiae Experimentalis, 2017

Research paper thumbnail of Induction of Brain Insulin Resistance and Alzheimer’s Molecular Changes by Western Diet

International Journal of Molecular Sciences

The term Western diet (WD) describes the consumption of large amounts of highly processed foods, ... more The term Western diet (WD) describes the consumption of large amounts of highly processed foods, rich in simple sugars and saturated fats. Long-term WD feeding leads to insulin resistance, postulated as a risk factor for Alzheimer’s disease (AD). AD is the main cause of progressive dementia characterized by the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles consisting of the hyperphosphorylated tau (p-Tau) protein in the brain, starting from the entorhinal cortex and the hippocampus. In this study, we report that WD-derived impairment in insulin signaling induces tau and Aβ brain pathology in wild-type C57BL/6 mice, and that the entorhinal cortex is more sensitive than the hippocampus to the impairment of brain insulin signaling. In the brain areas developing WD-induced insulin resistance, we observed changes in p-Tau(Thr231) localization in neuronal subcellular compartments, indicating progressive tauopathy, and a decrease in amyloid precursor protein levels corre...

Research paper thumbnail of Intraneuronal delivery of exogenous tau protein mediated by halitoxins may serve as a pharmacological animal model of human tauopathy in rats

Frontiers in Behavioral Neuroscience, 2009

The holotype of Amauroderma picipes, described by Torrend in 1920 from material collected in the ... more The holotype of Amauroderma picipes, described by Torrend in 1920 from material collected in the State of Bahia, Brazil, was recently located in herbarium URM, but it was in poor conditions. Due to the loss of the typical characteristics of the species, a neotype is designated here. The material chosen for A. picipes was collected in the State of Rio de Janeiro and deposited in herbarium SP (95472) as A. schomburgkii f. schomburgkii. A full modern description, pictures of the basidiomata and basidiospores in light microscopy and SEM are provided.

Research paper thumbnail of Review Tau Protein Modifications and Interactions: Their Role in Function and Dysfunction

Abstract: Tau protein is abundant in the central nervous system and involved in microtubule assem... more Abstract: Tau protein is abundant in the central nervous system and involved in microtubule assembly and stabilization. It is predominantly associated with axonal microtubules and present at lower level in dendrites where it is engaged in signaling functions. Post-translational modifications of tau and its interaction with several proteins play an important regulatory role in the physiology of tau. As a consequence of abnormal modifications and expression, tau is redistributed from neuronal processes to the soma and forms toxic oligomers or aggregated deposits. The accumulation of tau protein is increasingly recognized as the neuropathological hallmark of a number of dementia disorders known as tauopathies. Dysfunction of tau protein may contribute to collapse of cytoskeleton, thereby causing improper anterograde and retrograde movement of motor proteins and their cargos on microtubules. These disturbances in intraneuronal signaling may compromise synaptic transmission as well as tr...

Research paper thumbnail of Palm Oil-Rich Diet Affects Murine Liver Proteome and S-Palmitoylome

International Journal of Molecular Sciences

Palmitic acid (C16:0) is the most abundant saturated fatty acid in animals serving as a substrate... more Palmitic acid (C16:0) is the most abundant saturated fatty acid in animals serving as a substrate in synthesis and β-oxidation of other lipids, and in the modification of proteins called palmitoylation. The influence of dietary palmitic acid on protein S-palmitoylation remains largely unknown. In this study we performed high-throughput proteomic analyses of a membrane-enriched fraction of murine liver to examine the influence of a palm oil-rich diet (HPD) on S-palmitoylation of proteins. HPD feeding for 4 weeks led to an accumulation of C16:0 and C18:1 fatty acids in livers which disappeared after 12-week feeding, in contrast to an accumulation of C16:0 in peritoneal macrophages. Parallel proteomic studies revealed that HPD feeding induced a sequence of changes of the level and/or S-palmitoylation of diverse liver proteins involved in fatty acid, cholesterol and amino acid metabolism, hemostasis, and neutrophil degranulation. The HPD diet did not lead to liver damage, however, it ca...

Research paper thumbnail of Administration of clenbuterol improves morphology of cholinergic neurons and cognitive processes in aged rats

Acta Neurobiologiae Experimentalis, 2009

Research paper thumbnail of Lipopolysaccharide accelerates neuroinflammation in a mouse model of Alzheimer's disease

Acta Neurobiologiae Experimentalis, 2019

Research paper thumbnail of Influence of systemic inflammation and fluctuations of blood glucose level on amyloidopathy progression in mouse model of sporadic Alzheimer’s disease

Research paper thumbnail of Involvement of the CacyBP/SIP protein in tauopaty

Research paper thumbnail of Transmembrane intraneuronal delivery of Tau protein in rat may serve as an animal model of human tauopathy

Research paper thumbnail of Beyond amyloid:altered cell cycle regulation in Alzheimer's disease:comparison of P21 signaling in patients lymphocytes and brain neurons

Acta Neurobiologiae Experimentalis, 2015

Research paper thumbnail of Western diet as a trigger of Alzheimer’s disease: From metabolic syndrome and systemic inflammation to neuroinflammation and neurodegeneration

Research paper thumbnail of Western Diet Induces Impairment of Liver-Brain Axis Accelerating Neuroinflammation and Amyloid Pathology in Alzheimer's Disease

Frontiers in Aging Neuroscience

Alzheimer's disease (AD) is an aging-dependent, irreversible neurodegenerative disorder and t... more Alzheimer's disease (AD) is an aging-dependent, irreversible neurodegenerative disorder and the most common cause of dementia. The prevailing AD hypothesis points to the central role of altered cleavage of amyloid precursor protein (APP) and formation of toxic amyloid-β (Aβ) deposits in the brain. The lack of efficient AD treatments stems from incomplete knowledge on AD causes and environmental risk factors. The role of lifestyle factors, including diet, in neurological diseases is now beginning to attract considerable attention. One of them is western diet (WD), which can lead to many serious diseases that develop with age. The aim of the study was to investigate whether WD-derived systemic disturbances may accelerate the brain neuroinflammation and amyloidogenesis at the early stages of AD development. To verify this hypothesis, transgenic mice expressing human APP with AD-causing mutations (APPswe) were fed with WD from the 3rd month of age. These mice were compared to APPswe...

Research paper thumbnail of A New Inhibitor of Tubulin Polymerization Kills Multiple Cancer Cell Types and Reveals p21-Mediated Mechanism Determining Cell Death after Mitotic Catastrophe

Cancers

Induction of mitotic catastrophe through the disruption of microtubules is an established target ... more Induction of mitotic catastrophe through the disruption of microtubules is an established target in cancer therapy. However, the molecular mechanisms determining the mitotic catastrophe and the following apoptotic or non-apoptotic cell death remain poorly understood. Moreover, many existing drugs targeting tubulin, such as vincristine, have reduced efficacy, resulting from poor solubility in physiological conditions. Here, we introduce a novel small molecule 2-aminoimidazoline derivative—OAT-449, a synthetic water-soluble tubulin inhibitor. OAT-449 in a concentration range from 6 to 30 nM causes cell death of eight different cancer cell lines in vitro, and significantly inhibits tumor development in such xenograft models as HT-29 (colorectal adenocarcinoma) and SK-N-MC (neuroepithelioma) in vivo. Mechanistic studies showed that OAT-449, like vincristine, inhibited tubulin polymerization and induced profound multi-nucleation and mitotic catastrophe in cancer cells. HeLa and HT-29 cel...

Research paper thumbnail of Neuroplasticity and Neuroprotective Effect of Treadmill Training in the Chronic Mouse Model of Parkinson’s Disease

Neural Plasticity

Physical training confers protection to dopaminergic neurons in rodent models of parkinsonism pro... more Physical training confers protection to dopaminergic neurons in rodent models of parkinsonism produced by neurotoxins. The sparing effect of physical training on dopaminergic neurons can be tested with training applied during chronic MPTP treatment, while the neurorestorative effect when training is applied after completing such treatment. In this study, the effect of the onset of training respective to chronic MPTP treatment was specifically addressed. Three groups of mice were injected with 10 doses of MPTP (12.5 mg/kg/injection) over 5 weeks. The first group remained sedentary; the second one underwent early onset training, which started 1 week before commencing MPTP treatment, continued throughout 5 weeks of treatment and 4 weeks thereafter; the third group underwent late-onset training of the same length and intensity as the former group, except that it started immediately after the end of MPTP treatment. Two groups served as controls: a saline-injected group that remained sede...

Research paper thumbnail of Modulation of LPS-Induced Neurodegeneration by Intestinal Helminth Infection in Ageing Mice

International Journal of Molecular Sciences, Sep 11, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Greater sensitivity of the hippocampus than the entorhinal cortex in response to western diet, manifested by accelerated neuroinflammation and neurodegeneration in the progression of Alzheimer’s disease

Alzheimer's & Dementia, Nov 30, 2023

Research paper thumbnail of P4-003: Treatment with Lithium Chloride Alleviates Morphological and Cognitive Impairment Provoked by Excessive Tau in Pharmacologically Induced Rat Model of Human Tauopathy

Alzheimers & Dementia, Jul 1, 2014

WEDNESDAY, JULY 16, 2014 POSTER PRESENTATIONS P4 P4-001 CHANGES IN FRONTOCORTICAL INTERSTITIAL FL... more WEDNESDAY, JULY 16, 2014 POSTER PRESENTATIONS P4 P4-001 CHANGES IN FRONTOCORTICAL INTERSTITIAL FLUID LEVELS OF BETA-AMYLOID AND TAU PROTEIN IN TRANSGENIC MICE MODELS OF ALZHEIMER’S DISEASE Alain Gobert, Gaelle Rollin-Jego, Fabrice Iop, Jean Michel Rivet, Dominique Favale, Laurence Danober, Sophie Dix, Christian Czech, Ozmen Laurence, Jill Richardson, Esther Schenker, Institut de Recherches Servier, Croissy sur Seine, France; Institut de Recherches Servier, Croissy sur Seine, France; Institut de Recherches Servier, Croissy sur Seine, France; Eli Lilly, Lilly Research Centre, Surrey, United Kingdom; CNS Research, Hoffmann-La Roche AG, Basel, Switzerland; F. Hoffmann-LaRoche AG, Basel, Switzerland; GlaxoSmithKline Research and Development, China Group, Stevenage, United Kingdom; Institut de Recherches Servier, Croissy sur Seine, France. Contact e-mail: alain.gobert@fr.netgrs.com

Research paper thumbnail of Brain insulin resistance and Alzheimer’s molecular changes induced by Western diet

Alzheimer's & Dementia

BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simp... more BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simple sugars and saturated fats. Long‐term consumption of WD may lead to disruption of insulin signaling and development of insulin resistance. It is supposed that insulin resistance is a probable risk factors for Alzheimer’s disease (AD). The aim of this study was to verify this hypothesis in wild type mice by checking the WD effect on initiation and propagation of main neuropathological AD features such as amyloid‐β (Aβ) plaques and neurofibrillary tangles, which start from the entorhinal cortex in the temporal area, and progresses to the hippocampus, resulting in the loss of memory and cognition.MethodMales of wild type C57BL/6J mice were fed WD diet or standard diet (SD; CTR). Mice experimental groups (WD and CTR) were divided into age subgroups 4‐, 8‐, 12‐ and 16‐month‐old. In the first step, the WD‐diet dependent insulin signaling was analyzed, and the levels of the insulin pathway co...

Research paper thumbnail of Brain insulin resistance and Alzheimer’s molecular changes induced by Western diet

Alzheimer's & Dementia

BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simp... more BackgroundWestern diet (WD) is a type of nourishment based on ultra‐processed foods, rich in simple sugars and saturated fats. Long‐term consumption of WD may lead to disruption of insulin signaling and development of insulin resistance. It is supposed that insulin resistance is a probable risk factors for Alzheimer’s disease (AD). The aim of this study was to verify this hypothesis in wild type mice by checking the WD effect on initiation and propagation of main neuropathological AD features such as amyloid‐β (Aβ) plaques and neurofibrillary tangles, which start from the entorhinal cortex in the temporal area, and progresses to the hippocampus, resulting in the loss of memory and cognition.MethodMales of wild type C57BL/6J mice were fed WD diet or standard diet (SD; CTR). Mice experimental groups (WD and CTR) were divided into age subgroups 4‐, 8‐, 12‐ and 16‐month‐old. In the first step, the WD‐diet dependent insulin signaling was analyzed, and the levels of the insulin pathway co...

Research paper thumbnail of Long-term effects of subdural injections of an antlerogenic cell homogenate (ACH) in a porcine model of spinal cord injury (SCI)

Acta Neurobiologiae Experimentalis, 2017

Research paper thumbnail of Induction of Brain Insulin Resistance and Alzheimer’s Molecular Changes by Western Diet

International Journal of Molecular Sciences

The term Western diet (WD) describes the consumption of large amounts of highly processed foods, ... more The term Western diet (WD) describes the consumption of large amounts of highly processed foods, rich in simple sugars and saturated fats. Long-term WD feeding leads to insulin resistance, postulated as a risk factor for Alzheimer’s disease (AD). AD is the main cause of progressive dementia characterized by the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles consisting of the hyperphosphorylated tau (p-Tau) protein in the brain, starting from the entorhinal cortex and the hippocampus. In this study, we report that WD-derived impairment in insulin signaling induces tau and Aβ brain pathology in wild-type C57BL/6 mice, and that the entorhinal cortex is more sensitive than the hippocampus to the impairment of brain insulin signaling. In the brain areas developing WD-induced insulin resistance, we observed changes in p-Tau(Thr231) localization in neuronal subcellular compartments, indicating progressive tauopathy, and a decrease in amyloid precursor protein levels corre...

Research paper thumbnail of Intraneuronal delivery of exogenous tau protein mediated by halitoxins may serve as a pharmacological animal model of human tauopathy in rats

Frontiers in Behavioral Neuroscience, 2009

The holotype of Amauroderma picipes, described by Torrend in 1920 from material collected in the ... more The holotype of Amauroderma picipes, described by Torrend in 1920 from material collected in the State of Bahia, Brazil, was recently located in herbarium URM, but it was in poor conditions. Due to the loss of the typical characteristics of the species, a neotype is designated here. The material chosen for A. picipes was collected in the State of Rio de Janeiro and deposited in herbarium SP (95472) as A. schomburgkii f. schomburgkii. A full modern description, pictures of the basidiomata and basidiospores in light microscopy and SEM are provided.

Research paper thumbnail of Review Tau Protein Modifications and Interactions: Their Role in Function and Dysfunction

Abstract: Tau protein is abundant in the central nervous system and involved in microtubule assem... more Abstract: Tau protein is abundant in the central nervous system and involved in microtubule assembly and stabilization. It is predominantly associated with axonal microtubules and present at lower level in dendrites where it is engaged in signaling functions. Post-translational modifications of tau and its interaction with several proteins play an important regulatory role in the physiology of tau. As a consequence of abnormal modifications and expression, tau is redistributed from neuronal processes to the soma and forms toxic oligomers or aggregated deposits. The accumulation of tau protein is increasingly recognized as the neuropathological hallmark of a number of dementia disorders known as tauopathies. Dysfunction of tau protein may contribute to collapse of cytoskeleton, thereby causing improper anterograde and retrograde movement of motor proteins and their cargos on microtubules. These disturbances in intraneuronal signaling may compromise synaptic transmission as well as tr...

Research paper thumbnail of Palm Oil-Rich Diet Affects Murine Liver Proteome and S-Palmitoylome

International Journal of Molecular Sciences

Palmitic acid (C16:0) is the most abundant saturated fatty acid in animals serving as a substrate... more Palmitic acid (C16:0) is the most abundant saturated fatty acid in animals serving as a substrate in synthesis and β-oxidation of other lipids, and in the modification of proteins called palmitoylation. The influence of dietary palmitic acid on protein S-palmitoylation remains largely unknown. In this study we performed high-throughput proteomic analyses of a membrane-enriched fraction of murine liver to examine the influence of a palm oil-rich diet (HPD) on S-palmitoylation of proteins. HPD feeding for 4 weeks led to an accumulation of C16:0 and C18:1 fatty acids in livers which disappeared after 12-week feeding, in contrast to an accumulation of C16:0 in peritoneal macrophages. Parallel proteomic studies revealed that HPD feeding induced a sequence of changes of the level and/or S-palmitoylation of diverse liver proteins involved in fatty acid, cholesterol and amino acid metabolism, hemostasis, and neutrophil degranulation. The HPD diet did not lead to liver damage, however, it ca...

Research paper thumbnail of Administration of clenbuterol improves morphology of cholinergic neurons and cognitive processes in aged rats

Acta Neurobiologiae Experimentalis, 2009

Research paper thumbnail of Lipopolysaccharide accelerates neuroinflammation in a mouse model of Alzheimer's disease

Acta Neurobiologiae Experimentalis, 2019

Research paper thumbnail of Influence of systemic inflammation and fluctuations of blood glucose level on amyloidopathy progression in mouse model of sporadic Alzheimer’s disease

Research paper thumbnail of Involvement of the CacyBP/SIP protein in tauopaty

Research paper thumbnail of Transmembrane intraneuronal delivery of Tau protein in rat may serve as an animal model of human tauopathy

Research paper thumbnail of Beyond amyloid:altered cell cycle regulation in Alzheimer's disease:comparison of P21 signaling in patients lymphocytes and brain neurons

Acta Neurobiologiae Experimentalis, 2015

Research paper thumbnail of Western diet as a trigger of Alzheimer’s disease: From metabolic syndrome and systemic inflammation to neuroinflammation and neurodegeneration

Research paper thumbnail of Western Diet Induces Impairment of Liver-Brain Axis Accelerating Neuroinflammation and Amyloid Pathology in Alzheimer's Disease

Frontiers in Aging Neuroscience

Alzheimer's disease (AD) is an aging-dependent, irreversible neurodegenerative disorder and t... more Alzheimer's disease (AD) is an aging-dependent, irreversible neurodegenerative disorder and the most common cause of dementia. The prevailing AD hypothesis points to the central role of altered cleavage of amyloid precursor protein (APP) and formation of toxic amyloid-β (Aβ) deposits in the brain. The lack of efficient AD treatments stems from incomplete knowledge on AD causes and environmental risk factors. The role of lifestyle factors, including diet, in neurological diseases is now beginning to attract considerable attention. One of them is western diet (WD), which can lead to many serious diseases that develop with age. The aim of the study was to investigate whether WD-derived systemic disturbances may accelerate the brain neuroinflammation and amyloidogenesis at the early stages of AD development. To verify this hypothesis, transgenic mice expressing human APP with AD-causing mutations (APPswe) were fed with WD from the 3rd month of age. These mice were compared to APPswe...

Research paper thumbnail of A New Inhibitor of Tubulin Polymerization Kills Multiple Cancer Cell Types and Reveals p21-Mediated Mechanism Determining Cell Death after Mitotic Catastrophe

Cancers

Induction of mitotic catastrophe through the disruption of microtubules is an established target ... more Induction of mitotic catastrophe through the disruption of microtubules is an established target in cancer therapy. However, the molecular mechanisms determining the mitotic catastrophe and the following apoptotic or non-apoptotic cell death remain poorly understood. Moreover, many existing drugs targeting tubulin, such as vincristine, have reduced efficacy, resulting from poor solubility in physiological conditions. Here, we introduce a novel small molecule 2-aminoimidazoline derivative—OAT-449, a synthetic water-soluble tubulin inhibitor. OAT-449 in a concentration range from 6 to 30 nM causes cell death of eight different cancer cell lines in vitro, and significantly inhibits tumor development in such xenograft models as HT-29 (colorectal adenocarcinoma) and SK-N-MC (neuroepithelioma) in vivo. Mechanistic studies showed that OAT-449, like vincristine, inhibited tubulin polymerization and induced profound multi-nucleation and mitotic catastrophe in cancer cells. HeLa and HT-29 cel...

Research paper thumbnail of Neuroplasticity and Neuroprotective Effect of Treadmill Training in the Chronic Mouse Model of Parkinson’s Disease

Neural Plasticity

Physical training confers protection to dopaminergic neurons in rodent models of parkinsonism pro... more Physical training confers protection to dopaminergic neurons in rodent models of parkinsonism produced by neurotoxins. The sparing effect of physical training on dopaminergic neurons can be tested with training applied during chronic MPTP treatment, while the neurorestorative effect when training is applied after completing such treatment. In this study, the effect of the onset of training respective to chronic MPTP treatment was specifically addressed. Three groups of mice were injected with 10 doses of MPTP (12.5 mg/kg/injection) over 5 weeks. The first group remained sedentary; the second one underwent early onset training, which started 1 week before commencing MPTP treatment, continued throughout 5 weeks of treatment and 4 weeks thereafter; the third group underwent late-onset training of the same length and intensity as the former group, except that it started immediately after the end of MPTP treatment. Two groups served as controls: a saline-injected group that remained sede...