Anthony Ranchou-Peyruse - Academia.edu (original) (raw)

Papers by Anthony Ranchou-Peyruse

BSGF - Earth Sciences Bulletin

The Asal–Ghoubbet active rift in the Republic of Djibouti is a site of interest for geothermal en... more The Asal–Ghoubbet active rift in the Republic of Djibouti is a site of interest for geothermal energy and natural hydrogen, and previous studies have indicated that dihydrogen (H2) emanates from this rift. However, the well-known serpentinization reaction does not appear to be the main mechanism generating H2 at this site. Rather, the H2 is generated as follows: (1) by alteration of basaltic lava at depth via reaction with seawater flowing from Ghoubbet Bay towards Lake Asal; (2) by simple degassing of the volcanic chamber located a few kilometers below the Fiale Caldera in the rift axis; or (3) as a result of pyritization processes via the oxidation of H2S. Study of microorganisms did not indicate any production or consumption of H2, CO2, or CH4; therefore, it is unlikely that microorganisms affected H2 gas contents measured at the surface. However, air contamination at fumaroles is typically considerable and may limit interpretation of such processes. Drill cuttings from the Fiale...

HAL (Le Centre pour la Communication Scientifique Directe), May 24, 2017

Frontiers in Microbiology

To be effective, microbiological studies of deep aquifers must be free from surface microbial con... more To be effective, microbiological studies of deep aquifers must be free from surface microbial contaminants and from infrastructures allowing access to formation water (wellheads, well completions). Many microbiological studies are based on water samples obtained after rinsing a well without guaranteeing the absence of contaminants from the biofilm development in the pipes. The protocol described in this paper presents the adaptation, preparation, sterilization and deployment of a commercial downhole sampler (PDSshort, Leutert, Germany) for the microbiological studying of deep aquifers. The ATEX sampler (i.e., explosive atmospheres) can be deployed for geological gas storage (methane, hydrogen). To validate our procedure and confirm the need to use such a device, cell counting and bacterial taxonomic diversity based on high-throughput sequencing for different water samples taken at the wellhead or at depth using the downhole sampler were compared and discussed. The results show that ...

HAL (Le Centre pour la Communication Scientifique Directe), Sep 1, 2021

In this study, the challenge is to characterize biofilm growth in a natural aquifer, identified a... more In this study, the challenge is to characterize biofilm growth in a natural aquifer, identified as a potential future location for storing biogas. More precisely, we want to evaluate the impact of biofilm growth on the porosity by means of high-resolution X-ray computed tomography without using artificial contrast agents as these substances could affect the phenomenon of interest.

Les milieux cotiers sont regulierement exposes a des pollutions petrolieres ; une part importante... more Les milieux cotiers sont regulierement exposes a des pollutions petrolieres ; une part importante des composes de ce petrole se retrouve piegee dans les sediments et tend a persister en conditions anoxiques. Parmi les communautes bacteriennes colonisant ces milieux, certains microorganismes sont capables de degrader les hydrocarbures petroliers et participent ainsi a la bioremediation de ces ecosystemes. L'objectif de ce travail etait de comprendre le role et la cooperation des differents groupes metaboliques bacteriens anaerobies lors de la degradation de molecules d'hydrocarbures modeles (alcanes, HAPs). Pour cela, des communautes bacteriennes anaerobies degradant des hydrocarbures ont ete selectionnees en conditions anoxiques et de lumiere (cultures batch et continue) a partir de biofilms photosynthetiques se developpant a la surface de sediments lagunaires contaminees par des hydrocarbures petroliers (Etang de Berre, France). L'association de techniques moleculaires ...

Reaction Chemistry & Engineering, 2020

Micromodels experimentation for studying and understanding CO2 geological storage mechanisms at t... more Micromodels experimentation for studying and understanding CO2 geological storage mechanisms at the pore scale.

Applied and Environmental Microbiology, 2011

Knallgas bacteria are a physiologically defined group that is primarily studied using cultivation... more Knallgas bacteria are a physiologically defined group that is primarily studied using cultivation-dependent techniques. Given that current cultivation techniques fail to grow most bacteria, cultivation-independent techniques that selectively detect and identify knallgas bacteria will improve our ability to study their diversity and distribution. We used stable-isotope probing (SIP) to identify knallgas bacteria in rhizosphere soil of legumes and in a microbial mat from Obsidian Pool in Yellowstone National Park. When samples were incubated in the dark, incorporation of 13 CO 2 was H 2 dependent. SIP enabled the detection of knallgas bacteria that were not detected by cultivation, and the majority of bacteria identified in the rhizosphere soils were betaproteobacteria predominantly related to genera previously known to oxidize hydrogen. Bacteria in soil grew on hydrogen at concentrations as low as 100 ppm. A hydB homolog encoding a putative high-affinity NiFe hydrogenase was amplifie...

Goldschmidt2022 abstracts, 2022

Energy & Environmental Science

This first multidisciplinary study simulating the H2 arrival in deep aquifers used as geological ... more This first multidisciplinary study simulating the H2 arrival in deep aquifers used as geological storage shows the importance of microorganisms.

Goldschmidt2021 abstracts, 2021

Frontiers in Microbiology, 2021

Deep aquifers (up to 2km deep) contain massive volumes of water harboring large and diverse micro... more Deep aquifers (up to 2km deep) contain massive volumes of water harboring large and diverse microbial communities at high pressure. Aquifers are home to microbial ecosystems that participate in physicochemical balances. These microorganisms can positively or negatively interfere with subsurface (i) energy storage (CH4 and H2), (ii) CO2 sequestration; and (iii) resource (water, rare metals) exploitation. The aquifer studied here (720m deep, 37°C, 88bar) is naturally oligotrophic, with a total organic carbon content of <1mg.L−1 and a phosphate content of 0.02mg.L−1. The influence of natural gas storage locally generates different pressures and formation water displacements, but it also releases organic molecules such as monoaromatic hydrocarbons at the gas/water interface. The hydrocarbon biodegradation ability of the indigenous microbial community was evaluated in this work. The in situ microbial community was dominated by sulfate-reducing (e.g., Sva0485 lineage, Thermodesulfovibr...

Science of The Total Environment, 2021

The last few years have seen the proliferation of anaerobic digestion plants to produce biomethan... more The last few years have seen the proliferation of anaerobic digestion plants to produce biomethane. Oxygen (O2) traces added to biogas during the desulfurization process are co-injected in the gas network and can be stored in Underground Gas Storage (UGS). However, there are no data available for the undesirable effects of O2 on these anoxic environments, especially on deep aquifers. In addition to mineral alteration, O2 can have an impact on the anaerobic autochthonous microbial life. In our study, the storage conditions of an UGS aquifer were reproduced in a high-pressure reactor and bio-geo-chemical interactions between the aqueous, gas and solid phases were studied. Sulfate was depleted from the liquid phase for three consecutive times during the first 130 days of incubation reproducing the storage conditions (36 °C, 60 bar, methane with 1% CO2). Sulfate-reducers, such as Desulfovibrionaceae, were identified from the high-pressure system. Simulations with PHREEQC were used to determine the thermodynamic equilibrium to confirm any gas consumption. CO2 quantities decreased in the gas phase, suggesting its use as carbon source by microbial life. Benzene and toluene, hydrocarbons found in traces and known to be biodegradable in storages, were monitored and a decrease of toluene was revealed and associated to the Peptococcaceae family. Afterwards, O2 was added as 1% of the gas phase, corresponding to the maximum quantity found in biomethane after desulfurization process. Re-oxidation of sulfide to sulfate was observed along with the end of sulfate reducing activity and toluene biodegradation and the disappearance of most of the community. H2 surprisingly appeared and accumulated as soon as hydrogenotrophic sulfate-reducers decreased. H2 would be produced via the necromass fermentation accomplished by microorganisms able to resist the oxic conditions of 4.42·10-4 mol.Kgw-1 of O2. The solid phase composed essentially of quartz, presented no remarkable changes.

Goldschmidt Abstracts, 2020

Processes, 2020

Both molecular analyses and culture-dependent isolation were combined to investigate the diversit... more Both molecular analyses and culture-dependent isolation were combined to investigate the diversity of sulfate-reducing prokaryotes and explore their role in sulfides production in full-scale anaerobic digesters (Marrakech, Morocco). At global scale, using 16S rRNA gene sequencing, Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Synergistetes, and Euryarchaeota were the most dominant phyla. The abundance of Archaea (3.1–5.7%) was linked with temperature. The mcrA gene ranged from 2.18 × 105 to 1.47 × 107 gene copies.g−1 of sludge. The sulfate-reducing prokaryotes, representing 5% of total sequences, involved in sulfides production were Peptococcaceae, Syntrophaceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae, Desulfurellaceae, and Desulfobacteraceae. Furthermore, dsrB gene ranged from 2.18 × 105 to 1.92 × 107 gene copies.g−1 of sludge. The results revealed that exploration of diversity and function of sulfate-reducing bacteria may play a key role in decrea...

Water, 2020

BTEX (benzene, toluene, ethylbenzene, and the different xylene isomers), known for carcinogenic a... more BTEX (benzene, toluene, ethylbenzene, and the different xylene isomers), known for carcinogenic and neurotoxic effects, are common environmental contaminants. The first step for the development of the bioremediation technologies is the detection of intense microbial degradation in contaminated waters in the quest for the most active bacterial strains. This requires the multispecies analysis for BTEX metabolites which are considered as markers of microbial degradation. A direct (50 µL injection) HPLC–electrospray MS/MS analytical method was developed for the simultaneous analysis of 11 BTEX metabolites (o-, m-, p-toluic, salicylic, benzoate, benzyl, and phenyl succinic acids, 2-(1-phenylethyl)-, 2-(2-methylbenzyl), and 2-(3-methylbenzyl)-, 2-(4-methyl benzyl)-succinic acids) in bacterial cultures and ground waters down to 0.1 ng/mL. The optimization of the chromatographic conditions allowed for the resolution of position isomers of toluic and methylbenzyl-succinic acids. The stabilit...

Instrumentation Mesure Métrologie, 2020

Glass micro-capillaries are the simplest yet most versatile, robust, practical and cheap microflu... more Glass micro-capillaries are the simplest yet most versatile, robust, practical and cheap microfluidic devices. Their small size and high optical quality favour detailed investigation under the optical microscope. Here we first review some of their applications, such as determining contact angles and the observation of tenuous wetting films under harsh conditions of pressure and temperature. We further explore how an optical cusp formed by reflection off the inner wall of a glass capillary may be used to monitor the refractive index of its fluid content. Finally, we illustrate how the above advantages may be put to use in the study of extremophile microorganisms, for example in recreating under the microscope the conditions prevailing on the ocean floors. RÉSUMÉ : Les microcapillaires de verre sont les outils microfluidiques les plus simples, et né anmoins ils sont polyvalents, robustes et bon marché. Leur petite taille et grande qualité optique favorisent leur utilisation sous le microscope optique. Nous passons en revue quelques unes de leurs applications, comme la dé termination d'angles de contact et l'observation de films de mouillage en conditions sé vè res de pression et tempé rature. Nous explorons ensuite comment la caustique formé e par ré flexion sur la paroi interne du capillaire est relié e à l'indice de ré fraction du contenu fluide. Finalement, nous illustrons comment ces avantages peuvent servir à l'é tude d'organismes extré mophiles, par exemple en recré ant sous le microscope les conditions des fonds océ aniques.

Research in Microbiology, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

International Journal of Systematic and Evolutionary Microbiology, 2018

The strain BerOc1 T was isolated from brackish sediments contaminated with hydrocarbons and heavy... more The strain BerOc1 T was isolated from brackish sediments contaminated with hydrocarbons and heavy metals. This strain has been used as a model strain of sulfate-reducer to study the biomethylation of mercury. The cells are vibrio-shaped, motile and not sporulated. Phylogeny and physiological traits placed this strain within the genus Pseudodesulfovibrio. Optimal growth was obtained at 30 C, 1.5 % NaCl and pH 6.0-7.4. The estimated G+C content of the genomic DNA was 62.6 mol%. BerOc1 T used lactate, pyruvate, fumarate, ethanol and hydrogen. Terminal electron acceptors used were sulfate, sulfite, thiosulfate and DMSO. Only pyruvate could be used without a terminal electron acceptor. The major fatty acids were C 18 : 0 , anteiso-C 15 : 0 , C 16 : 0 and C 18 : 1 !7. The name Pseudodesulfovibrio hydrargyri sp. nov. is proposed for the type strain BerOc1 T (DSM 10384 T =JCM 31820 T).