Anthony Reginato - Academia.edu (original) (raw)

Papers by Anthony Reginato

Rheumatology, Aug 11, 2012

Journal of Cellular Physiology, 2019

Osteoarthritis and Cartilage, Jun 1, 1995

We examined in vitro collagen biosynthesis by organ cultures from human fetal epiphyseal growth p... more We examined in vitro collagen biosynthesis by organ cultures from human fetal epiphyseal growth plate cartilage. The biosynthetic products were characterized by NaCl fractional precipitation, limited proteolytic digestion, and sodium dodecyl sulfate,polyacrylamide slab gel electrophoresis. Organ cultures of human fetal epiphyseal growth plate cartilage synthesized large amounts of type X collagen in addition to type H, type IX, and type XI collagens. The individual polypeptide chains of human type X collagen migrated with an apparent M r of 45 kDa after proteolytic digestion with pepsin. The migration pattern of these molecules did not change when examined under reducing and nonreducing conditions, indicating that they did not contain intrahelical disulfide bonds. Comparison of the rates at type X collagen biosynthesis at weeks 20 and 24 of human fetal development showed a marked increase of 24 weeks. Northern hybridization analysis of total RNA from freshly isolated epiphyseal growth plate'chondrocytes with a cDNA corresponding to the carboxyl terminus of human type X collagen indicated that the developmental increase of type X collagen production is determined by pre-translational mechanisms.

Seminars in Arthritis and Rheumatism, Dec 1, 2011

We describe 4 patients who presented with palpable purpura, arthralgia or arthritis, leukopenia, ... more We describe 4 patients who presented with palpable purpura, arthralgia or arthritis, leukopenia, and antineutrophil cytoplasmic antigen (ANCA) positivity most likely as a result of a hypersensitivity reaction to cocaine-levamisole induced vasculopathy. Cases were seen and reviewed in both the inpatient consult service and the outpatient clinics at Rhode Island Hospital from August 2009 to August 2010. Clinical characteristics as well as pertinent laboratory parameters were also reviewed and corroborated with a review of the present literature. We describe 3 cases of cocaine-levamisole-related cutaneous vasculitis with or without associated neutropenia, and 1 case of severe neutropenia with oral mucosal ulceration. Further serologic studies revealed maximum titers of ANCA mostly in a perinuclear pattern. Antimyeloperoxidase tested negative or mildly elevated in our cohort. Three patients with neutropenia had positive antigranulocyte IgM antibody. Nonsteroidal anti-inflammatory drugs were effective as first-line treatment for joint pain. The use of colchicine and systemic corticosteroid was employed to manage severe and persistent skin lesions. Cocaine-levamisole-related cutaneous vasculitis with leukopenia is a diagnosis of exclusion, but this diagnosis should be strongly considered in patients with a history of cocaine abuse who present with a tetrad of cutaneous manifestations consisting of palpable purpura or bullae with ear involvement, arthralgias, leukopenia, and positive ANCA in high titers and negative Antimyeloperoxidase, when other infectious or idiopathic vasculitic entities have been excluded.

In vitro cellular & developmental biology, Apr 1, 1992

We have developed a serum-free system to culture postembryonic growth plate chondrocytes while ma... more We have developed a serum-free system to culture postembryonic growth plate chondrocytes while maintaining some important phenotypic characteristics of their tissue of origin. This serum-free medium was as effective as medium containing 10% newborn bovine serum (NBS) for recovering the cells from enzymatic isolation. Surface secretory activity of ehondrocytes cultured in monolayer, assessed through scanning electron microscopy, was also comparable to cells grown in medium containing serum. The effects of growth hormone (GH) and insulinlike growth factor-I (IGF-I) were also studied using the serum-free medium. GH had no effect on cell density and morphology of the cells compared to the control without the hormone. In contrast, ehondrocytes grown in medium containing IGF-I had a marked increase in cell density after 3 days and presented similar morphologic characteristics to cells grown in the presence of NBS. The growth factors required for proliferation of chondrocytes cultured in the serum-free medium are IGF-I and fibroblast growth factor (100 ng/ml, respectively). Addition of ascorbic acid to the serum-free medium (0 to 50 #g/ml) produced a dose-dependant decrease in cell proliferation. This medium should provide a useful tool for studying the effects of different growth factors/hormones in the regulation of longitudinal bone growth and their interactions.

Journal of Biological Chemistry, Sep 1, 1989

A series of cDNA clones for the human core protein of the large cartilage-specific proteoglycan w... more A series of cDNA clones for the human core protein of the large cartilage-specific proteoglycan was isolated. Nucleotide sequencing of the clones provided over 2 kilobases of new coding sequences for the human protein. Comparison with published data for cDNA clones covering the same region in rat and chick indicated that domain 8, the lectin-like domain, is highly conserved among species. In contrast, domain 7 is poorly conserved among species. Some of the cDNA clones also contained an additional structural domain between domains 7 and 8 which was not described in the rat or chick sequences. The additional domain of 38 amino acids was highly homologous to epidermal growth factor (EGF)-like sequences seen in other proteins. Because some cDNA clones contained codons for the EGF-like domain and some did not, the results suggested that the EGF-like domain underwent alternative RNA splicing. To confirm alternative splicing of the EGF-like domain, RNA from cartilage cells was used as a template for the polymerase chain reaction. Products of two sizes were obtained. One had the size predicted for mRNA containing the domain and the other had the size predicted for mRNA not containing the domain. Alternative splicing of an EGF-like domain may provide a mechanism of feedback regulation for both the biosynthetic activity and the proliferation of cartilage cells.

Development, Apr 15, 2002

Best Practice & Research: Clinical Rheumatology, Dec 1, 2020

Current Rheumatology Reports, Jul 1, 2006

Cleveland Clinic Journal of Medicine, Apr 1, 2018

A 50-year-old woman with hypertension presents with a history of polyarticular small-joint pain f... more A 50-year-old woman with hypertension presents with a history of polyarticular small-joint pain for the last 3 months. Her pain is worse in the morning, and it affects her metacarpal, proximal, and distal phalangeal joints. She describes intermittent swelling of her hands and morning stiffness lasting 15 to 30 minutes.

Current Rheumatology Reports, Jun 1, 2008

Clinical Rheumatology, Nov 4, 2016

Primary hyperparathyroidism (PHPT) can be associated with a variety of musculoskeletal complaints... more Primary hyperparathyroidism (PHPT) can be associated with a variety of musculoskeletal complaints, which occasionally can be the leading or presenting manifestation. In this paper, we describe the musculoskeletal manifestations observed in patients with primary hyperparathyroidism. Medical record reviews of a select population of 74 patients with primary hyperparathyroidism are seen in a rheumatology practice. Bone manifestations included back pain in 11 patients (15.2 %), generalized bone pain in 7 patients (9.7 %), rib cage/chest pain in 6 (8.3 %), pseudoclubbing in 3, and a giant cell tumor of the mandible in 2 (2.3 %) patients. Articular manifestations such as chondrocalcinosis with or without apatite deposition disease were seen in 13 (17.7 %), arthralgias in 11 (15.2 %), and non-specific synovitis in 7 (9.7 %). Muscle weakness was observed in six patients (8.3 %) and myalgias in three (4.6 %). Less common manifestations such as Achilles tendon rupture, Jaccoud-like arthropathy, sacral insufficiency fracture, arthritis associated with fever of unknown origin (FUO), meningitis, cervical cord compression, and persistent headache were observed in single patients. Musculoskeletal findings are still a frequent and important presentation in patients with primary hyperparathyroidism seen in rheumatology practice. Some of these manifestations can be quite unusual and may represent diagnostic dilemmas to the practicing rheumatologist and/or endocrinologist.

Annual Review of Cell and Developmental Biology, Nov 1, 2000

▪ Early development of the vertebrate skeleton depends on genes that pattern the distribution a... more ▪ Early development of the vertebrate skeleton depends on genes that pattern the distribution and proliferation of cells from cranial neural crest, sclerotomes, and lateral plate mesoderm into mesenchymal condensations at sites of future skeletal elements. Within these condensations, cells differentiate to chondrocytes or osteoblasts and form cartilages and bones under the control of various transcription factors. In most of the skeleton, organogenesis results in cartilage models of future bones; in these models cartilage is replaced by bone by the process of endochondral ossification. Lastly, through a controlled process of bone growth and remodeling the final skeleton is shaped and molded. Significant and exciting insights into all aspects of vertebrate skeletal development have been obtained through molecular and genetic studies of animal models and humans with inherited disorders of skeletal morphogenesis, organogenesis, and growth.

$Research paper thumbnail of POS0267 Mortality Outcome of Acute Myocardial Infraction and Acute Heart Failure Hospitalization in Rheumatic Diseases Patients PRE-COVID-19 vs. COVID-19 Pandemic from 2016-2020 National Inpatient Sample (Nis) Database$

Annals of the Rheumatic Diseases, May 30, 2023

cytotoxic T cells are considered as essential components of the antiviral defense arsenal, and si... more cytotoxic T cells are considered as essential components of the antiviral defense arsenal, and since it is not clear when to do booster shots, analysis of thespecific T response over the long term could be a useful decision-making tool. Objectives: This study aims to compare the spike-specific T lymphocytes response in a cohort of Rheumatoid Arthritis (RA) patients under RTX or other therapies (csDMARDs, anti-TNF). Our secondary goal was to assess the retention of this response up to 18 months after the last COVID boost (vaccine or infection). Methods: Patients. Our study cohort included 75 consecutive adult patients with ACPA positive RA, followed at the rheumatology department of Sainte Marguerite Hospital (Marseille, France), prospectively enrolled from April 2022 to October 2022. RA patients fulfilled the 2010 ACR/EULAR criteria for RA and had received at least two doses of SARS-COV-2 mRNA vaccine, whether they had or not a history of COVID. Samples. Heparin-anticoagulated single blood sample was collected for each patient to assess CD19+ cell count, IgG level, and SARS-CoV-2 serology. Cellular immune response was assessed by flow cytometry with a previously described procedure [4]. Briefly, 250 µL of whole blood were incubated per condition, including a negative control, Spike peptides from JPT* collection and CEFX peptides (mix of viral peptides) as a positive control. Marker expression was measured with a three-laser 13-color CytoFLEX flow cytometer. T lymphocytes were divided into T4 lymphocytes (LT4) or T8 lymphocytes (LT8) depending on CD8 expression. Finally, CD69, CD154, CD137 and CD107a expressions were monitored to characterize LT4 or LT8 activation. Ethics. All patients gave informed written consent for this study in accordance with Helsinki declaration. Patient data were pseudo-anonymized. Sample collection was approved by the national ethics committee under the number DC-2008-327. Results: Patient characteristics: 51 RA patients were treated with RTX, 24 were treated with csDMARDs or other bDMARDs. Humoral response against SARS CoV 2: 30/51 (59%) RA RTX patients versus 13/14 (93%) non RTX patients had a humoral immune response (p = 0.024) with a median titer of 130 BAU/mL versus 688 BAU/mL. T cell specific response: LT4 and LT8 Spike-specific responses were defined by the difference of response between Spike peptides from SARS CoV 2 stimulation and no peptides stimulation. The response was divided into quartiles, patients in the upper 3 quartiles were considered to have a specific response. In RA RTX patients, specific LT8 response was shown in 90% of patients versus 42% in non RTX patients (p < 0.0001), and specific LT4 response was shown in 76 % of patients versus 75% in non RTX patients (p = 0.42). Long term T cell specific response (Figure 1). RA patients treated with RTX maintained a specific LT4 and LT8 response against Spike peptides with no decrease up to 18 months after the last SARS-COV-2 boost (vaccine dose or COVID 19 infection). Conclusion: Specific LT4 response against Spike peptides was similar in the RTX treated and non-treated RA patients. This was even stronger for the specific LT8 response. The Spike specific T-cell response was maintained in both groups up to 18 months after the last vaccine dose or COVID infection independently to the specific humoral response. This method of analyzing the specific T response against the Spike protein could be used in personalized medicine to decide when revaccination is necessary in a given patient.

Oxford University Press eBooks, Jul 1, 2014

Acute monoarticular arthritis represents one of the few rheumatologic emergencies that internists... more Acute monoarticular arthritis represents one of the few rheumatologic emergencies that internists, emergency room physicians, and rheumatologists will encounter in their clinical practice. The possibility of joint infection leading to the loss of joint function is a serious potential consequence in delayed diagnosis of a septic joint. Despite advances in diagnosis and treatment, the morbidity and mortality from septic arthritis remains high; therefore, timely recognition of an infected joint is imperative for a favorable outcome. The differential diagnosis of acute monoarticular arthritis is broad and includes septic arthritis, crystalline disorders, inflammatory disorders, and mechanical problems with the joint (table 24.1).

As the population ages and persons live longer with more medical comorbidities, the incidences of... more As the population ages and persons live longer with more medical comorbidities, the incidences of both gout and pseudogout will continue to increase. Uric acid metabolism is crucial to the pathogenesis of gout. Patients who have the arthritis associated with crystal-deposition disease typically present clinically with acute attacks of joint pain, swelling, and erythema and have asymptomatic periods between acute attacks.

Elsevier eBooks, 2001

Publisher Summary Recent advances in developmental biology, skeletal cell biology, and molecular ... more Publisher Summary Recent advances in developmental biology, skeletal cell biology, and molecular genetics have increased the understanding of bone development significantly. The development of the vertebrate skeleton depends on the regulated differentiation, function, and interactions of its cellular components. Current understanding of the molecular pathways that control patterning, growth, and differentiation of the various skeletal elements has been derived from a combination of genetics, experimental developmental biology, and in vitro cellular and biochemical studies. Knowledge of embryonic skeletal development provides a better basis for generating strategies to repair cartilage and bone in patients with skeletal diseases such as osteoporosis. Fibroblast growth factors (FGFs) are essential for several aspects of bone development, not only in the ossification of cranial sutures and limb bud outgrowth, but also in growth plate function, that is, longitudinal bone growth. FGFs have been shown to support the proliferation of a variety of mesenchymal and epithelial cells; regulate cell migration, differentiation, and chemotaxis; and be involved in a variety of non-skeletal and skeletal developmental processes. The roles of extracellular matrix proteins, not only as structural components, but also as modulators of signal transduction have come into sharper focus recently. However, much needs to be accomplished in skeletal biology, including a better understanding of endochondral ossification and the function of cartilage in bone remodeling and the factors that control cortical and trabecular bone thickness.

Current treatment options in rheumatology, May 27, 2017