Antonella Canini - Academia.edu (original) (raw)

Papers by Antonella Canini

Energies, 2021

Our world is facing an environmental crisis that is driving scientists to research green and smar... more Our world is facing an environmental crisis that is driving scientists to research green and smart solutions in terms of the use of renewable energy sources and low polluting technologies. In this framework, photovoltaic (PV) technology is one of the most worthy of interest. Dye-sensitized solar cells (DSSCs) are innovative PV devices known for their encouraging features of low cost and easy fabrication, good response to diffuse light and colour tunability. All these features make DSSCs technology suitable for being applied to the so-called agrovoltaic field, taking into account their dual role of filtering light and supporting energy needs. In this project, we used 40 DSSC Z-series connected modules with the aim of combining the devices’ high conversion efficiency, transparency and robustness in order to test them in a greenhouse. A maximum conversion efficiency of 3.9% on a 221 cm2 active area was achieved with a transparency in the module’s aperture (312.9 cm2) area of 35%. Moreo...

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011

This study was carried out to evaluate the antimicrobial and anti-inflammatory activity of some c... more This study was carried out to evaluate the antimicrobial and anti-inflammatory activity of some chemical compounds of the volatile oil extracted from Tropaeolum majus L. The chemical compounds extracted from the flowers and leaves of T. majus L. have been identified through color reactions and gas-chromatographic analysis combined with mass spectrometry. Quantitative testing and the ascertaining of the minimum inhibitory concentration (MIC) have been done through the binary micro dilution method for liquid environments against the following microbial types and species. The qualitative evaluation of the sensitivity of microbial stems against these compounds has been done through methods that have been adapted from the diffusimetric method. Of the qualitative methods used for the control of the antimicrobial activity, the method of diffusion on filter paper discs proved to be the most efficient, the results correlating well with the MIC. Our studies have demonstrated the efficiency of the natural compounds' of T. majus L. in anti-inflammatory treatments in animals. The antimicrobial activity proved to be selective, depending on the pathogen. These results are in agreement with those of other studies. Our results supported the selection and utilization of these compounds' as antimicrobial agents in the treatment of infections with microorganisms resistant to existent antibiotics.

Foods

Plant secondary metabolites possess chemopreventive and antineoplastic properties, but the lack o... more Plant secondary metabolites possess chemopreventive and antineoplastic properties, but the lack of information about their exact mechanism of action in mammalian cells hinders the translation of these compounds in suitable therapies. In light of this, firstly, Origanum vulgare L. hydroalcoholic extract was chemically characterized by spectrophotometric and chromatographic analyses; then, the molecular bases underlying its antitumor activity on B16-F10 and A375 melanoma cells were investigated. Oregano extract induced oxidative stress and inhibited melanogenesis and tumor cell proliferation, triggering programmed cell death pathways (both apoptosis and necroptosis) through mitochondria and DNA damage. By contrast, oregano extract was safe on healthy tissues, revealing no cytotoxicity and mutagenicity on C2C12 myoblasts, considered as non-tumor proliferating cell model system, and on Salmonella strains, by the Ames test. All these data provide scientific evidence about the potential a...

Plants

Plant records reveal remarkable evidence about past environments and human cultures. Exploiting d... more Plant records reveal remarkable evidence about past environments and human cultures. Exploiting dental calculus analysis and using a combined approach of microscopy and gas chromatography mass spectrometry, our research outlines dietary ecology and phytomedicinal practices of the ancient community of Motya (Sicily, eight to sixth century BC), one of the most important Phoenician settlements in the Mediterranean basin. Micro-remains suggest use or consumption of Triticeae cereals, and animal-derived sources (e.g., milk and aquatic birds). Markers of grape (or wine), herbs, and rhizomes, endemic of Mediterranean latitudes and the East, provide insight into the subsistence of this colony, in terms of foodstuffs and phytotherapeutic products. The application of resins and wood of Gymnosperms for social and cultural purposes is hypothesized through the identification of Pinaceae secondary metabolites and pollen grains. The information hidden in dental calculus discloses the strong human-...

Experimental and Therapeutic Medicine

Moringa oleifera Lam. (MO) is one of the most well-known and widely distributed species of the Mo... more Moringa oleifera Lam. (MO) is one of the most well-known and widely distributed species of the Moringaceae family in African communities, and various preparations of M. oleifera are used for the treatment of several diseases. Due to the extensive worldwide use of MO products, and the use of MO aqueous extract in traditional African medicine, the aim of the present study was to investigate the anti-proliferative, cytotoxic and pro-apoptotic activities of different aqueous extracts from leaves and seeds of M. oleifera (MOE), which have been prepared using different protocols, in lymphoid and monocytoid cells. The results of the present study demonstrated the anti-proliferative and pro-apoptotic effects of the aqueous extracts obtained from M. oleifera leaves and seeds on tumour cells; however, not on peripheral blood mononuclear cells (PBMCs) from healthy donors. The pro-apoptotic effect of MO seed aqueous extract (MOE-S) was correlated with decreased B-cell lymphoma 2 (BCL2) and sirtuin-1 (SIRT1) protein expression, which are involved in apoptosis. Considering the effects of plant secondary metabolites on human cells and the role of plant microRNA in cross-kingdom interactions, the presence of secondary metabolites and microRNA in MOE was characterised. In conclusion, M. oleifera aqueous extracts appeared to be able to differentially regulate proliferation and apoptosis in healthy cells and cancer cells, and this ability could be associated with the microRNA present in the extracts. These results highlighted the possible use of MOE as an adjuvant in traditional cancer therapy. Recently, particular attention has been provided to study the effects on human health of natural substances present in Mediterranean diet and their bioactive compounds demonstrating anticancer, antioxidant, antimicrobial and antiviral properties of olive oil correlated with the activity of phenolic and polyphenolic compounds present in it .

Algological Studies/Archiv für Hydrobiologie, Supplement Volumes

Archaeological and Anthropological Sciences

Communications Biology

There is a growing interest in therapeutically targeting the inflammatory response that underlies... more There is a growing interest in therapeutically targeting the inflammatory response that underlies age-related chronic diseases including obesity and type 2 diabetes. Through integrative small RNA sequencing, we show the presence of conserved plant miR159a and miR156c in dried nuts having high complementarity with the mammalian TNF receptor superfamily member 1a (Tnfrsf1a) transcript. We detected both miR159a and miR156c in exosome-like nut nanovesicles (NVs) and demonstrated that such NVs reduce Tnfrsf1a protein and dampen TNF-α signaling pathway in adipocytes. Synthetic single-stranded microRNAs (ss-miRs) modified with 2′-O-methyl group function as miR mimics. In plants, this modification naturally occurs on nearly all small RNAs. 2′-O-methylated ss-miR mimics for miR156c and miR159a decreased Tnfrsf1a protein and inflammatory markers in hypertrophic as well as TNF-α-treated adipocytes and macrophages. miR156c and miR159a mimics effectively suppress inflammation in mice, highlighting a potential role of plant miR-based, single-stranded oligonucleotides in treating inflammatory-associated metabolic diseases.

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology

Molecules

Antibacterial activity is the most investigated biological property of honey. The goal of this st... more Antibacterial activity is the most investigated biological property of honey. The goal of this study was to evaluate the antibacterial activity of 57 Slovak blossom honeys against Staphylococcus aureus and Pseudomonas aeruginosa and investigate the role of several bioactive substances in antibacterial action of honeys. Inhibitory and bactericidal activities of honeys were studied to determine the minimum inhibitory and bactericidal concentrations. The contents of glucose oxidase (GOX) enzyme, hydrogen peroxide (H2O2), and total polyphenols (TP) were determined in honeys. We found that honey samples showed different antibacterial efficacy against the tested bacteria as follows: wildflower honeys > acacia honeys > rapeseed honeys. Overall antibacterial activity of the honeys was statistically-significantly correlated with the contents of H2O2 and TP in honeys. A strong correlation was found between the H2O2 and TP content. On the other hand, no correlation was found between the ...

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology

ABSTRACT

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology

Journal of Plant Research

Medicinal Chemistry Research

PLOS ONE

Baldoni, Marica, Scorrano, Gabriele, Gismondi, Angelo et al. (7 more authors) (2018) Who were the... more Baldoni, Marica, Scorrano, Gabriele, Gismondi, Angelo et al. (7 more authors) (2018) Who were the miners of Allumiere? A multidisciplinary approach to reconstruct the osteobiography of an Italian worker community. PLOS one. e0205362.

Journal of the science of food and agriculture, Jan 10, 2018

Robinia pseudoacacia L. nectar and its derivative monofloral honey were systematically compared i... more Robinia pseudoacacia L. nectar and its derivative monofloral honey were systematically compared in this study, to understand how much the starting solution reflected the final product, after re-elaboration by Apis mellifera ligustica Spinola. Subjected to dehydration in the hive, nectar changed its water and sugar content when transformed in honey, as physicochemical and GC-MS analyses revealed. Spectrophotometrical measurements and HPLC-DAD characterization of 18 plant molecules demonstrated honey to be richer than nectar in secondary metabolites. For the first time, the hypothesis of the existence of a nectar redox cycle in R. pseudoacacia was reported, as previously described for Nicotiana sp., based on 1D-protein profiles, WB analysis and detection of H2 O2 and ascorbate. The bioactivity of both matrixes was also investigated. Antiradical in vitro tests showed that Acacia honey is more antioxidant than nectar, which was even able to induce oxidative stress directly on a eukaryot...

World Journal of Pharmaceutical Research

Sida acuta is a shrub indigenous to pantropical regions. The plant is widely used for its various... more Sida acuta is a shrub indigenous to pantropical regions. The plant is widely used for its various pharmacological properties. Among compounds of pharmacological interest occurring in the plant, are indoloquinoline alkaloids. The aim of the present study was to investigate the antimicrobial activity of alkaloids of S. acuta from Burkina Faso. The alkaloids had a good antimicrobial activity against the test microorganisms. In the agar-well diffusion assay, highest inhibition zone diameters were recorded with Gram-positive bacteria. The broth microdilution assay gave minimal inhibitory concentration values ranging from 16 to 400 µg/ml and minimal bactericidal concentration values ranging from 80 to up to 400 µg/ml. The gas chromatography-mass spectrometry analysis of the same alkaloids led to the identification of cryptolepine and quindoline as the major components.

Molecular Microbiology

Previous studies have suggested that P. aeruginosa possesses redundant zinc uptake systems. To id... more Previous studies have suggested that P. aeruginosa possesses redundant zinc uptake systems. To identify uncharacterized zinc transporters, we analyzed the genome-wide transcriptional responses of P. aeruginosa PA14 to zinc restriction. This approach led to the identification of an operon (zrmABCD) regulated by the zinc uptake regulator Zur, that encodes for a metallophore-mediated zinc import system. This operon includes the genes for an uncharacterized TonB-dependent Outer Membrane Protein (ZrmA) and for a putative nicotianamine synthase (ZrmB). The simultaneous inactivation of the ZnuABC transporter and of one of these two genes markedly decreases the ability of P. aeruginosa to grow in zinc-poor media and compromises intracellular zinc accumulation. Our data demonstrate that ZrmB is involved in the synthesis of a metallophore which is released outside the cell and mediates zinc uptake through the ZrmA receptor. We also show that alterations in zinc homeostasis severely affect the ability of P. aeruginosa to cause acute lung and systemic infections in C57BL/6 mice, likely due to the involvement of zinc in the expression of several virulence traits. These findings disclose a hitherto unappreciated role of zinc in P. aeruginosa pathogenicity and reveal that this microorganism can obtain zinc through a strategy resembling siderophoremediated iron uptake.

Oncology reports, Jan 24, 2017

Royal jelly (RJ) is one the most important bee product because it strongly influences the larval ... more Royal jelly (RJ) is one the most important bee product because it strongly influences the larval development in the hive, including the queen bee. In literature, RJ is known for its antioxidant, immunoregulatory, antifungal, antibiotical, erythropoietic, hypoglycemic, anticholesteremic, antithyroidic, anti-osteoporotic and estrogenic properties. However, it is surprising how rare the scientific evidence about RJ antineoplastic capacity are. That being said, we investigated, for the first time, the in vitro bioactivity of six different RJs on the growth of three different mammalian cell lines: immortalized murine myoblasts (C2C12), human prostate cancer (PC3) and human neuroblastoma (SH-SY5Y). These studies were performed treating the cells with the only lipophilic, or hydrophilic, fraction of the RJs, a scientific approach never performed before. Moreover, chemical and protein profiles of all RJs were finely characterized, in qualitative and quantitative terms, by GC-MS and 1D-SDS-P...

Energies, 2021

Our world is facing an environmental crisis that is driving scientists to research green and smar... more Our world is facing an environmental crisis that is driving scientists to research green and smart solutions in terms of the use of renewable energy sources and low polluting technologies. In this framework, photovoltaic (PV) technology is one of the most worthy of interest. Dye-sensitized solar cells (DSSCs) are innovative PV devices known for their encouraging features of low cost and easy fabrication, good response to diffuse light and colour tunability. All these features make DSSCs technology suitable for being applied to the so-called agrovoltaic field, taking into account their dual role of filtering light and supporting energy needs. In this project, we used 40 DSSC Z-series connected modules with the aim of combining the devices’ high conversion efficiency, transparency and robustness in order to test them in a greenhouse. A maximum conversion efficiency of 3.9% on a 221 cm2 active area was achieved with a transparency in the module’s aperture (312.9 cm2) area of 35%. Moreo...

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011

This study was carried out to evaluate the antimicrobial and anti-inflammatory activity of some c... more This study was carried out to evaluate the antimicrobial and anti-inflammatory activity of some chemical compounds of the volatile oil extracted from Tropaeolum majus L. The chemical compounds extracted from the flowers and leaves of T. majus L. have been identified through color reactions and gas-chromatographic analysis combined with mass spectrometry. Quantitative testing and the ascertaining of the minimum inhibitory concentration (MIC) have been done through the binary micro dilution method for liquid environments against the following microbial types and species. The qualitative evaluation of the sensitivity of microbial stems against these compounds has been done through methods that have been adapted from the diffusimetric method. Of the qualitative methods used for the control of the antimicrobial activity, the method of diffusion on filter paper discs proved to be the most efficient, the results correlating well with the MIC. Our studies have demonstrated the efficiency of the natural compounds' of T. majus L. in anti-inflammatory treatments in animals. The antimicrobial activity proved to be selective, depending on the pathogen. These results are in agreement with those of other studies. Our results supported the selection and utilization of these compounds' as antimicrobial agents in the treatment of infections with microorganisms resistant to existent antibiotics.

Foods

Plant secondary metabolites possess chemopreventive and antineoplastic properties, but the lack o... more Plant secondary metabolites possess chemopreventive and antineoplastic properties, but the lack of information about their exact mechanism of action in mammalian cells hinders the translation of these compounds in suitable therapies. In light of this, firstly, Origanum vulgare L. hydroalcoholic extract was chemically characterized by spectrophotometric and chromatographic analyses; then, the molecular bases underlying its antitumor activity on B16-F10 and A375 melanoma cells were investigated. Oregano extract induced oxidative stress and inhibited melanogenesis and tumor cell proliferation, triggering programmed cell death pathways (both apoptosis and necroptosis) through mitochondria and DNA damage. By contrast, oregano extract was safe on healthy tissues, revealing no cytotoxicity and mutagenicity on C2C12 myoblasts, considered as non-tumor proliferating cell model system, and on Salmonella strains, by the Ames test. All these data provide scientific evidence about the potential a...

Plants

Plant records reveal remarkable evidence about past environments and human cultures. Exploiting d... more Plant records reveal remarkable evidence about past environments and human cultures. Exploiting dental calculus analysis and using a combined approach of microscopy and gas chromatography mass spectrometry, our research outlines dietary ecology and phytomedicinal practices of the ancient community of Motya (Sicily, eight to sixth century BC), one of the most important Phoenician settlements in the Mediterranean basin. Micro-remains suggest use or consumption of Triticeae cereals, and animal-derived sources (e.g., milk and aquatic birds). Markers of grape (or wine), herbs, and rhizomes, endemic of Mediterranean latitudes and the East, provide insight into the subsistence of this colony, in terms of foodstuffs and phytotherapeutic products. The application of resins and wood of Gymnosperms for social and cultural purposes is hypothesized through the identification of Pinaceae secondary metabolites and pollen grains. The information hidden in dental calculus discloses the strong human-...

Experimental and Therapeutic Medicine

Moringa oleifera Lam. (MO) is one of the most well-known and widely distributed species of the Mo... more Moringa oleifera Lam. (MO) is one of the most well-known and widely distributed species of the Moringaceae family in African communities, and various preparations of M. oleifera are used for the treatment of several diseases. Due to the extensive worldwide use of MO products, and the use of MO aqueous extract in traditional African medicine, the aim of the present study was to investigate the anti-proliferative, cytotoxic and pro-apoptotic activities of different aqueous extracts from leaves and seeds of M. oleifera (MOE), which have been prepared using different protocols, in lymphoid and monocytoid cells. The results of the present study demonstrated the anti-proliferative and pro-apoptotic effects of the aqueous extracts obtained from M. oleifera leaves and seeds on tumour cells; however, not on peripheral blood mononuclear cells (PBMCs) from healthy donors. The pro-apoptotic effect of MO seed aqueous extract (MOE-S) was correlated with decreased B-cell lymphoma 2 (BCL2) and sirtuin-1 (SIRT1) protein expression, which are involved in apoptosis. Considering the effects of plant secondary metabolites on human cells and the role of plant microRNA in cross-kingdom interactions, the presence of secondary metabolites and microRNA in MOE was characterised. In conclusion, M. oleifera aqueous extracts appeared to be able to differentially regulate proliferation and apoptosis in healthy cells and cancer cells, and this ability could be associated with the microRNA present in the extracts. These results highlighted the possible use of MOE as an adjuvant in traditional cancer therapy. Recently, particular attention has been provided to study the effects on human health of natural substances present in Mediterranean diet and their bioactive compounds demonstrating anticancer, antioxidant, antimicrobial and antiviral properties of olive oil correlated with the activity of phenolic and polyphenolic compounds present in it .

Algological Studies/Archiv für Hydrobiologie, Supplement Volumes

Archaeological and Anthropological Sciences

Communications Biology

There is a growing interest in therapeutically targeting the inflammatory response that underlies... more There is a growing interest in therapeutically targeting the inflammatory response that underlies age-related chronic diseases including obesity and type 2 diabetes. Through integrative small RNA sequencing, we show the presence of conserved plant miR159a and miR156c in dried nuts having high complementarity with the mammalian TNF receptor superfamily member 1a (Tnfrsf1a) transcript. We detected both miR159a and miR156c in exosome-like nut nanovesicles (NVs) and demonstrated that such NVs reduce Tnfrsf1a protein and dampen TNF-α signaling pathway in adipocytes. Synthetic single-stranded microRNAs (ss-miRs) modified with 2′-O-methyl group function as miR mimics. In plants, this modification naturally occurs on nearly all small RNAs. 2′-O-methylated ss-miR mimics for miR156c and miR159a decreased Tnfrsf1a protein and inflammatory markers in hypertrophic as well as TNF-α-treated adipocytes and macrophages. miR156c and miR159a mimics effectively suppress inflammation in mice, highlighting a potential role of plant miR-based, single-stranded oligonucleotides in treating inflammatory-associated metabolic diseases.

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology

Molecules

Antibacterial activity is the most investigated biological property of honey. The goal of this st... more Antibacterial activity is the most investigated biological property of honey. The goal of this study was to evaluate the antibacterial activity of 57 Slovak blossom honeys against Staphylococcus aureus and Pseudomonas aeruginosa and investigate the role of several bioactive substances in antibacterial action of honeys. Inhibitory and bactericidal activities of honeys were studied to determine the minimum inhibitory and bactericidal concentrations. The contents of glucose oxidase (GOX) enzyme, hydrogen peroxide (H2O2), and total polyphenols (TP) were determined in honeys. We found that honey samples showed different antibacterial efficacy against the tested bacteria as follows: wildflower honeys > acacia honeys > rapeseed honeys. Overall antibacterial activity of the honeys was statistically-significantly correlated with the contents of H2O2 and TP in honeys. A strong correlation was found between the H2O2 and TP content. On the other hand, no correlation was found between the ...

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology

ABSTRACT

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology

Journal of Plant Research

Medicinal Chemistry Research

PLOS ONE

Baldoni, Marica, Scorrano, Gabriele, Gismondi, Angelo et al. (7 more authors) (2018) Who were the... more Baldoni, Marica, Scorrano, Gabriele, Gismondi, Angelo et al. (7 more authors) (2018) Who were the miners of Allumiere? A multidisciplinary approach to reconstruct the osteobiography of an Italian worker community. PLOS one. e0205362.

Journal of the science of food and agriculture, Jan 10, 2018

Robinia pseudoacacia L. nectar and its derivative monofloral honey were systematically compared i... more Robinia pseudoacacia L. nectar and its derivative monofloral honey were systematically compared in this study, to understand how much the starting solution reflected the final product, after re-elaboration by Apis mellifera ligustica Spinola. Subjected to dehydration in the hive, nectar changed its water and sugar content when transformed in honey, as physicochemical and GC-MS analyses revealed. Spectrophotometrical measurements and HPLC-DAD characterization of 18 plant molecules demonstrated honey to be richer than nectar in secondary metabolites. For the first time, the hypothesis of the existence of a nectar redox cycle in R. pseudoacacia was reported, as previously described for Nicotiana sp., based on 1D-protein profiles, WB analysis and detection of H2 O2 and ascorbate. The bioactivity of both matrixes was also investigated. Antiradical in vitro tests showed that Acacia honey is more antioxidant than nectar, which was even able to induce oxidative stress directly on a eukaryot...

World Journal of Pharmaceutical Research

Sida acuta is a shrub indigenous to pantropical regions. The plant is widely used for its various... more Sida acuta is a shrub indigenous to pantropical regions. The plant is widely used for its various pharmacological properties. Among compounds of pharmacological interest occurring in the plant, are indoloquinoline alkaloids. The aim of the present study was to investigate the antimicrobial activity of alkaloids of S. acuta from Burkina Faso. The alkaloids had a good antimicrobial activity against the test microorganisms. In the agar-well diffusion assay, highest inhibition zone diameters were recorded with Gram-positive bacteria. The broth microdilution assay gave minimal inhibitory concentration values ranging from 16 to 400 µg/ml and minimal bactericidal concentration values ranging from 80 to up to 400 µg/ml. The gas chromatography-mass spectrometry analysis of the same alkaloids led to the identification of cryptolepine and quindoline as the major components.

Molecular Microbiology

Previous studies have suggested that P. aeruginosa possesses redundant zinc uptake systems. To id... more Previous studies have suggested that P. aeruginosa possesses redundant zinc uptake systems. To identify uncharacterized zinc transporters, we analyzed the genome-wide transcriptional responses of P. aeruginosa PA14 to zinc restriction. This approach led to the identification of an operon (zrmABCD) regulated by the zinc uptake regulator Zur, that encodes for a metallophore-mediated zinc import system. This operon includes the genes for an uncharacterized TonB-dependent Outer Membrane Protein (ZrmA) and for a putative nicotianamine synthase (ZrmB). The simultaneous inactivation of the ZnuABC transporter and of one of these two genes markedly decreases the ability of P. aeruginosa to grow in zinc-poor media and compromises intracellular zinc accumulation. Our data demonstrate that ZrmB is involved in the synthesis of a metallophore which is released outside the cell and mediates zinc uptake through the ZrmA receptor. We also show that alterations in zinc homeostasis severely affect the ability of P. aeruginosa to cause acute lung and systemic infections in C57BL/6 mice, likely due to the involvement of zinc in the expression of several virulence traits. These findings disclose a hitherto unappreciated role of zinc in P. aeruginosa pathogenicity and reveal that this microorganism can obtain zinc through a strategy resembling siderophoremediated iron uptake.

Oncology reports, Jan 24, 2017

Royal jelly (RJ) is one the most important bee product because it strongly influences the larval ... more Royal jelly (RJ) is one the most important bee product because it strongly influences the larval development in the hive, including the queen bee. In literature, RJ is known for its antioxidant, immunoregulatory, antifungal, antibiotical, erythropoietic, hypoglycemic, anticholesteremic, antithyroidic, anti-osteoporotic and estrogenic properties. However, it is surprising how rare the scientific evidence about RJ antineoplastic capacity are. That being said, we investigated, for the first time, the in vitro bioactivity of six different RJs on the growth of three different mammalian cell lines: immortalized murine myoblasts (C2C12), human prostate cancer (PC3) and human neuroblastoma (SH-SY5Y). These studies were performed treating the cells with the only lipophilic, or hydrophilic, fraction of the RJs, a scientific approach never performed before. Moreover, chemical and protein profiles of all RJs were finely characterized, in qualitative and quantitative terms, by GC-MS and 1D-SDS-P...