Anupong Tankrathok - Academia.edu (original) (raw)

Papers by Anupong Tankrathok

Journal of King Saud University. Science/Maǧallaẗ ǧāmiʹaẗ al-malik Saʹūd. al-ʹUlūm, Apr 1, 2024

Veterinary World, Oct 31, 2023

Background and Aim: Antibiotic resistance is a major global health threat. The increasing prevale... more Background and Aim: Antibiotic resistance is a major global health threat. The increasing prevalence of drug-resistant bacteria poses a serious challenge to the effective treatment of infections in both humans and animals. Water is a major source of human and animal exposure to bacteria, and the presence of drug-resistant bacteria in water could present a severe threat to public health and animal production. This study investigated the presence of drug-resistant bacteria in Lam Pao Dam (LPD) water in Kalasin, Thailand. Materials and Methods: Ampicillin-resistant strains were obtained from LPD water and identified using 16s rDNA sequencing. Antibiotic resistance genes were detected by polymerase chain reaction using specific primers. The presence of antibiotic-resistant bacteria was evaluated using 16s amplicon analysis. The minimum inhibitory concentration (MIC) of Raoultella ornithinolytica strains against antibiotics was determined. Results: A total of 12 R. ornithinolytica, 4 Bacillus cereus, and 4 Enterococcus faecalis isolates were resistant to ampicillin. Almost all R. ornithinolytica strains harbored bla SHV and bla OXA genes, and two strains also harbored the bla NDM-1 gene. All four E. faecalis strains harbored the bla IMP gene. The most abundant species in the LPD sample was Exiguobacterium indicum, followed by E. faecalis and R. ornithinolytica. The MICs of 10 R. ornithinolytica strains against five antibiotics revealed that all strains were resistant to ampicillin but susceptible to meropenem, doripenem, ertapenem, and imipenem. Conclusion: These findings suggest a high prevalence of drug-resistant bacteria in LPD water. This is a cause for concern, as it could spread antibiotic-resistant infections in the community.

Process Biochemistry, Aug 1, 2023

Melioidosis is an emerging infectious disease in India and caused by gram-negative, soil saprophy... more Melioidosis is an emerging infectious disease in India and caused by gram-negative, soil saprophyte bacteria Burkholderia pseudomallei. This disease is endemic in Southeast Asia and northern Australia, and sporadic cases of melioidosis are also reported from southern states of India. The present study reports the cloning, expression, and purification of recombinant protein outer membrane protein A (OmpA) of B. pseudomallei and its evaluation in indirect enzyme-linked immunosorbent assay (ELISA) format with 87 serum samples collected from Manipal, Karnataka, India. Twenty-three samples from culture confirmed cases (n = 23) of melioidosis, 25 serum samples from patients of other febrile illness and pyrexia of unknown origin (n = 25), and 39 serum samples from healthy blood donors (n = 39) from Kasturba Medical College, Manipal, were tested in this assay format. The assay showed sensitivity of 82.6% and specificity of 93.75%. The recombinant OmpA based indirect ELISA will be a useful tool for serodiagnosis of melioidosis in large scale rapid screening of clinical samples.

Journal of Bioscience and Bioengineering, Sep 1, 2020

A simple toolbox was developed for the evaluation of tyrosinase inhibitory activity in Salak (Sal... more A simple toolbox was developed for the evaluation of tyrosinase inhibitory activity in Salak (Salacca zalacca) extracts by the colorimetric measurement based on a photograph taken by a digital camera or a smartphone camera. The reaction of 3,4-dihydroxyphenylalanine (l-DOPA) and tyrosinase was employed to form the dopaquinone dye, which decreases with the increase of the tyrosinase inhibitor. Under the optimum conditions, the Salak extracts were examined for the tyrosinase inhibitory activity. The captured picture of dopaquinone dye product was analyzed by reading blue color intensity using an Adobe Photoshop CS6 program. The tyrosinase inhibition of the extracts was calculated from the blue color intensity, and expressed as %inhibition and IC 50 values. The obtained results from the developed method correlated well with those obtained from the microplate reader instrument. The achievement of this research will be a guideline for creating any simple analytical instrument based on colorimetry. In addition, the information on tyrosinase inhibitory activity of the Salak extracts is useful for the application of this fruit to produce the supplement food and the cosmetic in the future.

Veterinary World, Aug 13, 2021

Background and Aim: Parasitic infections are one of the major problems to the production of cattl... more Background and Aim: Parasitic infections are one of the major problems to the production of cattle in Thailand. The study was conducted to determine the prevalence of gastrointestinal (GI) parasites of cattle in Kalasin Province, Thailand. Materials and Methods: A total of 333 fecal samples of cattle were collected directly from the rectum. The fecal samples were subjected to formalin-ethyl acetate concentration methods for examination. The eggs or oocysts were identified based on the morphology and size of the eggs or oocysts. Results: Out of 333 fecal samples examined, 320 were found positive for GI parasitic infections with a prevalence of 96.09%. Overall, among the prevalence of nematodes, trematodes, and protozoa, the most prevalent parasites were Strongyle-type 278 (84.24%), followed by Strongyloides spp. 54 (16.36%) and Trichuris spp. 75 (22.73%), while Protozoan oocyst recorded Eimeria spp. amounted to 131 (39.7%). Fasciola spp. and Paramphistomum spp. were 67 (20.30%) and 81 (24.55%), respectively. Most of the positive fecal samples were infected with the double infection which has the highest prevalence rate of about 40.24%, followed by single, three, and 4-5 types of parasites 30.63, 16.82, and 7.21%, respectively. Conclusion: This study suggests that Kalasin Province is highly endemic for GI parasites and this area may be an important source for an outbreak. Therefore, every household should deworm its cattle and eliminate and control snails as intermediate hosts. Findings from this study provide information that will assist in improving the cattle in Kalasin Province for better production and higher profitability.

Cathelicidins, a group of vertebrate multifunctional molecules, play a role in innate immunity. C... more Cathelicidins, a group of vertebrate multifunctional molecules, play a role in innate immunity. Cathelicidins are antimicrobial peptides (AMPs) that are involved in protection against microbial invasion. Presently, cathelicidin peptides have been identified from only 14 amphibian species. In the study, a novel cathelicidin was identified from the lungs of frogs, Hoplobatrachus rugulosus. A 474 base pairs (bp) complementary DNA (cDNA) sequence encoded a 157 amino acid residue prepropeptide of H. rugulosus cathelicidin (cathelicidin-HR), which consisting of a 20-residue signal peptide sequence, a 108-residue cathelin region, and a 29-residue cathelicidin peptide (PC29). Amino acid sequence alignment and cladogram analysis illustrated that cathelicidin-HR have a high degree of similarity to further amphibian cathelicidins. The PC29 peptide displays antimicrobial activity only against Bacillus subtilis and Enterococcus faecalis. However, the PC29 peptide performed dose-dependent antioxi...

Scientific Reports, Sep 25, 2023

Antimicrobial resistance is a growing health concern. Antimicrobial peptides are a potential solu... more Antimicrobial resistance is a growing health concern. Antimicrobial peptides are a potential solution because they bypass conventional drug resistance mechanisms. Previously, we isolated a peptide from Crocodylus siamensis hemoglobin hydrolysate, which has antimicrobial activity and identified the main peptide from this mixture (QL17). The objective of this work was to evaluate and rationally modify QL17 in order to: (1) control its mechanism of action through bacterial membrane disruption; (2) improve its antimicrobial activity; and (3) ensure it has low cytotoxicity against normal eukaryotic cells. QL17 was rationally designed using physicochemical and template-based methods. These new peptide variants were assessed for: (1) their in vitro inhibition of microbial growth, (2) their cytotoxicity against normal cells, (3) their selectivity for microbes, and (4) the mode of action against bacteria using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal microscopy. The results indicate that all designed peptides have more potent antimicrobial efficacy than QL17 and IL15 peptides. However, only the most rationally modified peptides showed strong antimicrobial activity and minimal toxicity against normal cells. In particular, IL15.3 (hydrophobicity of 47% and net charge of + 6) was a potent antimicrobial agent (MIC = 4-12 μg/mL; MBC = 6-25 μg/mL) and displayed excellent selectivity for microbes (cf. human cells) via FACS assays. Microscopy confirmed that IL15.3 acts against bacteria by disrupting the cell membrane integrity and penetrating into the membrane. This causes the release of intracellular content into the outer environment leading to the death of bacteria. Moreover, IL15.3 can also interact with DNA suggesting it could have dual mode of action. Overall, a novel variant of QL17 is described that increases antimicrobial activity by over 1000-fold (~ 5 μg/mL MIC) and has minimal cytotoxicity. It may have applications in clinical use to treat and safeguard against bacteria. Antimicrobial resistance is a global health concern; the poor adherence to drug courses, the misuse of drugs and improper disposal of drugs has resulted in microbes gradually developing resistance to existing drugs 1. It is therefore vital to identify new drugs, and especially those with novel mechanisms of action 2. To this end, antimicrobial peptides (AMPs) are promising as: (1) they typically act by disrupting bacterial membranes instead of inhibiting cellular growth (an unconventional mode of action); (2) they are short and are therefore easy to

Applied Biochemistry and Biotechnology

Journal of applied biology and biotechnology, Jun 1, 2022

Cathelicidins, a group of vertebrate multifunctional molecules, play a role in innate immunity. I... more Cathelicidins, a group of vertebrate multifunctional molecules, play a role in innate immunity. In this study, a cathelicidin was identified from the lungs of frogs, Hoplobatrachus rugulosus. A 474 base pairs complementary DNA sequence encoded a 157 amino acid residue prepropeptide of H. rugulosus cathelicidin (cathelicidin-HR), which consisting of a 20-residue signal peptide sequence, a 108-residue cathelin region, and a 29-residue cathelicidin-HR peptide. Amino acid sequence alignment and cladogram analysis illustrated that cathelicidin-HR have a high degree of similarity to other amphibian cathelicidins. The cathelicidin-HR peptide displays very low antimicrobial activity but exhibits dose-dependent antioxidant activity. Moreover, this peptide expresses DNA damage inhibition against UV/H 2 O 2-induction. The molecular docking indicated that DNA damage protection of cathelicidin-HR might occur via DNA-peptide complex formation. This is the first amphibian cathelicidin peptide that possesses DNA damage inhibitory activity which might play a crucial role in oxidative stress.

In a previous study, we reported an antibacterial peptide derived from Crocodylus siamensis hemog... more In a previous study, we reported an antibacterial peptide derived from Crocodylus siamensis hemoglobin hydrolysate, named QL17 (QAIIHNEKVQAHGKKVL); however t h i s peptide has a narrow spectrum of activity. To improve the antimicrobial activity of the peptide, it was used as the template to design novel effective peptides. The helical wheel diagram was used to monitor and evaluate hydrophobicity and hydrophilicity after the positional change and substitution of certain amino acids. Lysine (K) and arginine (R) were appropriately selected to extend the hydrophilicity, whereas hydrophobic residues such as leucine (L), isoleucine (I) or tryptophan (W) were used to increase the hydrophobicity. As appropriate, two novel peptides were synthesized and named as IL-K (IKHWKKVWKHWKKKL) and IL-R (IRHWRRVWRHWRRRL), which had the same hydrophobicity and net charge at 40% and +7, respectively. Evaluation of the antimicrobial activity by broth microdilution assay revealed that IL-K had a slightly h...

The purpose of this study was to assess the genetic diversity of 12 banana cultivars using inter ... more The purpose of this study was to assess the genetic diversity of 12 banana cultivars using inter simple sequence repeat (ISSR) markers. Twenty ISSR primers were used for screening, seven primers produced 54 total bands and 53 polymorphic bands with an average percentage of polymorphism of 96.43%. The average polymorphism information content (PIC), the average effective multiplex ratio (EMR), the average marker index (MI) and the average resolving power (Rp) were 0.26, 7.46, 2.08 and 4.10 respectively.Genetic similarity matrix from Pearson coefficient between the cultivars ranged from 0.010 to 0.657, with an average of 0.454. The lowest was between Kluai Sao Kratueb Ho and Kluai Hin while the highest was between Kluai Hak Muk Som and Kluai Nam Wa Kom. Cluster analysis using the unweighted pair group method with arithmetic mean analysis (UPGMA) divided 12 cultivars into two major groups. The first group included the main cultivar in this study while the second group comprised of only ...

Veterinary World, 2021

Background and Aim: Fasciola spp. are important foodborne trematodes and waterborne zoonotic para... more Background and Aim: Fasciola spp. are important foodborne trematodes and waterborne zoonotic parasites that cause health problems and economic losses worldwide, including in Thailand. Fasciola spp. are usually detected by sedimentation or the formalin-ethyl acetate concentration technique (FECT) under microscopy, which is less specific and sensitive. Accurate detection is important to detect real incidence for protection against and elimination of fasciolosis in the area. This study aimed to determine the distribution of Fasciola spp. and compare the specificity and sensitivity of FECT under microscopy to that of polymerase chain reaction (PCR) in cattle feces. Materials and Methods: The study was conducted in Kalasin Province, Thailand. Feces of 46 cattle were investigated for infection with Fasciola spp. To detect infection, FECT under microscopy and PCR amplification of the 28S rRNA gene of Fasciola spp. were used to identify egg parasites. Results: Feces of 16 of 46 (34.78%) cat...

Chemical Papers, 2018

Biotechnology and Applied Biochemistry, 2019

Cathelicidins are a class of antimicrobial peptides that play a critical role in vertebrate immun... more Cathelicidins are a class of antimicrobial peptides that play a critical role in vertebrate immunity against the pathogen. The work presents a novel cathelicidin from the blood of the Siamese crocodile. This work uses bioinformatics tools to predict a candidate antimicrobial peptide derived from Crocodylus siamensis cathelicidin amino acid sequence. The RN15 peptide located in the conserved cathelin region performs the antibacterial activity with low hemolytic activity and low cytotoxicity. This is the first report of cathelicidin from C. siamensis, and it is highlighted that its derived peptide from cathelin domain promises an excellent candidate for new peptide therapeutics templates.

Biotechnology and Applied Biochemistry, 2018

Cathelicidins are effector molecules of vertebrate immunity that play vital roles against microbi... more Cathelicidins are effector molecules of vertebrate immunity that play vital roles against microbial invasion. They are widely identified in the mammal, but few have been reported in Crocodilians with considered as powerful immune system species. In the present study, we 2 This article is protected by copyright. All rights reserved. identified and characterized a novel cathelicidin from the blood of Siamese crocodile, Crocodylus siamensis. A cDNA sequence (501 base pair (bp)) encodes a predicted 166residue prepropeptide of C. siamensis cathelicidin (Cs-CATH), which comprised of a 21residue signal peptide, a 109-residue cathelin domain, and a 36-residue mature cathelicidin peptide. The multiple sequence alignment and phylogenetic analysis demonstrated that Cs-CATH shared a high degree of similarity with other crocodylian cathelicidins. Joint consideration of elastase cleavage site, physicochemical properties and predicted secondary structure demonstrated RN15 peptide represents a candidate antimicrobial peptide derived from Cs-CATH. The synthetic RN15 peptide possesses antimicrobial activity against Grampositive and Gram-negative bacteria. Scanning electron microscopy (SEM) illustrated RN15 peptide induced bacteria cells exhibited morphological change. Besides, RN15 peptide performs low hemolytic activity against human erythrocytes and low cytotoxic activity against normal human dermal fibroblasts (NHDF). This is the first cathelicidin identified from C. siamensis and highlighted that its derived peptide from cathelin domain promising potent novel peptide antibiotics templates.

ACS chemical biology, Jul 26, 2016

Human glucosylcerebrosidase 2 (GBA2) of CAZy family GH116 is responsible for breakdown of glycosp... more Human glucosylcerebrosidase 2 (GBA2) of CAZy family GH116 is responsible for breakdown of glycosphingolipids on the cytoplasmic face of the endoplasmic reticulum and Golgi apparatus. Genetic defects in GBA2 result in spastic paraplegia and cerebellar ataxia, while cross-talk between GBA2 and GBA1 glucosylceramidases may affect Gaucher disease. Here we report the first three-dimensional structure for any GH116 enzyme, Thermoanaerobacterium xylanolyticum TxGH116 β-glucosidase, alone and in complex with diverse ligands. These structures allow identification of the glucoside binding and active site residues, which are shown to be conserved with GBA2. Mutagenic analysis of TxGH116 and structural modeling of GBA2 provides a detailed structural and functional rationale for pathogenic missense mutations of GBA2.