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Papers by Ana Aranha

Brazilian dental science, Dec 15, 2017

Brazilian Oral Research, 2013

This study evaluated the effects of the photoactivation source and restorative material on the de... more This study evaluated the effects of the photoactivation source and restorative material on the development of caries-like lesions on human enamel after an in vitro pH challenge. Enamel cavities were prepared in 36 blocks, which were assigned to two groups according to the restorative material: resin-modified glass ionomer (RMGI) and composite resin (CR). Samples were exposed to quartz-tungsten-halogen lamp, argon-ion laser, or light-emitting diode (n = 6). The Knoop microhardness (KHN) values of the top surface of all materials were evaluated. Restored enamel blocks were thermocycled and subjected to 10 demineralization-remineralization cycles at 37°C. KHN analysis of the superficial enamel was performed by four indentations located 100 µm from the restoration margin. The material KHN was not affected by the photoactivation source. No significant difference in KHN was noted between CR and RMGI. The enamel surface around RMGI exhibited a higher KHN (272.8 KHN) than the enamel around CR (93.3 KHN), regardless of the photoactivation source. Enamel demineralization around the dental restoration was not influenced by the photoactivation source. Less enamel demineralization was observed around the RMGI than around the CR restoration.

Lasers in Medical Science, 2010

The objective of this study was to evaluate the influence of Er:YAG laser (λ = 2.94 μm) on microt... more The objective of this study was to evaluate the influence of Er:YAG laser (λ = 2.94 μm) on microtensile bond strength (μTBS) and superficial morphology of bovine dentin bleached with 16% carbamide peroxide. Forty bovine teeth blocks (7 × 3 × 3 mm(3)) were randomly assigned to four groups: G1- bleaching and Er:YAG irradiation with energy density of 25.56 J/cm(2) (focused mode); G2 - bleaching; G3 - no-bleaching and Er:YAG irradiation (25.56 J/cm(2)); G4 - control, non-treated. G1 and G2 were bleached with 16% carbamide peroxide for 6 h during 21 days. Afterwards, all blocks were abraded with 320 to 600-grit abrasive papers to obtain flat standardized dentin surfaces. G1 and G3 were Er:YAG irradiated. Blocks were immediately restored with 4-mm-high composite resin (Adper Single Bond 2, Z-250-3 M/ESPE). After 24 h, the restored blocks (n = 9) were serially sectioned and trimmed to an hour-glass shape of approximately 1 mm(2) at the bonded interface area, and tested in tension in a universal testing machine (1 mm/ min). Failure mode was determined at a magnification of 100× using a stereomicroscope. One block of each group was selected for scanning electron microscope (SEM) analysis. μTBS data was analyzed by two-way ANOVA and Tukey test (α = 0.05). Mean bond strengths (SD) in MPa were: G1- 32.7 (5.9)(A); G2- 31.1 (6.3)(A); G3- 25.2 (8.3)(B); G4- 36.7 (9.9).(A) Groups with different uppercase letters were significantly different from each other (p < .05). Enamel bleaching procedure did not affect μTBS values for dentin adhesion. Er:YAG laser irradiation with 25.56 J/cm(2) prior to adhesive procedure of bleached teeth did not affect μTBS at dentin and promoted a dentin surface with no smear layer and opened dentin tubules observed under SEM. On the other hand, Er:YAG laser irradiation prior to adhesive procedure of non-bleached surface impaired μTBS compared to the control group.

International Journal of Adhesion and Adhesives, 2012

Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three... more Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three adhesive systems (AS) on dentin surfaces treated with Er:YAG laser using two irradiation parameters. Study Design: Occlusal flat dentin surfaces of 36 human third molars were assigned into nine groups (n ¼ 4) according to the following ASs: one bottle etch&rinse Single Bond Plus (3M ESPE), two-step Clearfil Protect Bond (Kuraray), and all-in-one S 3 Bond (Kuraray) self-etching, which were labeled with rhodamine B or fluorescein isothiocyanate-dextran and were applied to dentin surfaces that were irradiated with Er:YAG laser at either 120 (38.7 J/cm 2) or 200 mJ/pulse (64.5 J/cm 2), or were applied to untreated dentin surfaces (control group). The ASs were light-activated following MI and the bonded surfaces were restored with resin composite Z250 (3M ESPE). After 24 hours of storage in vegetable oil, the restored teeth were vertically, serially sectioned into 1-mm thick slabs, which had the adhesive interfaces analyzed with confocal laser microscope (CLSM-LSM 510 Meta). CLSM images were recorded in the fluorescent mode from three different regions along each bonded interface. Results: Non-uniform HL was created on laser-irradiated dentin surfaces regardless of laser irradiation protocol for all AS, while regular and uniform HL was observed in the control groups. ''Stretch mark''-like red lines were found within the HL as a result of resin infiltration into dentin microfissures, which were predominantly observed in 200 mJ/pulse groups regardless of AS. Poor resin infiltration into peritubular dentin was observed in most regions of adhesive interfaces created by all ASs on laser-irradiated dentin, resulting in thin resin tags with neither funnelshaped morphology nor lateral resin projections. Conclusion: Laser irradiation of dentin surfaces at 120 or 200 mJ/pulse resulted in morphological changes in HL and resin tags for all ASs evaluated in the study.

INTRODUCTION The purpose of a desensitizing agent is a permanent coating or filling of dentin sur... more INTRODUCTION The purpose of a desensitizing agent is a permanent coating or filling of dentin surface. Morphological analysis in vitro of this treated surface is essential to understand the interaction between desensitizing agent and hypersensitive dentin. The aim was to evaluate the morphology of four dentin surface treated with desensitizing agents. METHODS This was an in vitro laboratory study, where fifteen specimens from extracted human premolars were obtained. The enamel was removed to expose the dentin surface, polished with silicon carbide abrasive papers and etched with 6% citric acid for 2 min.The specimens were randomly divided into 5 groups: G1 - without treatment (control) (C), G2 - fluoride varnish (FV), G3 - potassium oxalate (PO), G4 - 2-step self-etching adhesive system (AS), G5 - diode laser (DL). The specimens were cleaved in the lingual buccaldirection, prepared for analysis by Scanning Electron Microscope and the surface and interior of the dentinal tubules were...

Photodiagnosis and photodynamic therapy, Jan 28, 2017

Chronic gingivostomatitis in dogs is an inflammatory syndrome of the oral cavity, which treatment... more Chronic gingivostomatitis in dogs is an inflammatory syndrome of the oral cavity, which treatment and control of concomitant periodontitis allow healing in most of the cases. In the presence of recurrent lesions, invasive methods are necessary to treat lesions and pain. As a conservative adjuvant method, photobiomodulation (PBM) with low power laser is able to promote reduction of tissue pain and tissue inflammation besides increasing vascularization and healing, restoring the normal function of the irradiated organ in a shorter time. In veterinary medicine, there is no standardization of technique for its use in oral tissue for treating gingivostomatitis in dogs. In the present case, a dog was submitted to aPDT (7.2J/point, 3min/point, 180J/cm(2)) and PBM (1.6J/point, 40sec/point, 25J/cm(2)), using a semiconductor diode laser, with wavelength of 660nm, spot size of ​​0.04cm(2) and output power of 40mW. The established protocol proved to be effective as coadjutant treatment for chro...

Lasers in medical science, 2017

This randomized and longitudinal in vivo study aimed to assess different protocols for the treatm... more This randomized and longitudinal in vivo study aimed to assess different protocols for the treatment of dentin hypersensitivity with low-power laser (with different doses), high-power laser, and a desensitizing agent, for a period of 12 and 18 months. The lesions from 32 patients (117 lesions), who were submitted to the inclusion and exclusion criteria, were divided into nine groups (n = 13): G1: Gluma Desensitizer (Heraeus Kulzer), G2: low-power laser with low dose (three points of irradiation in vestibular portion and an apical point 30 mW, 10 J/cm(2), 9 s per point with the wavelength of 810 nm, with three sessions with an interval of 72 h), G3: low-power laser with high dose (one point in the cervical area, and one apical point 100 mW, 40 J/cm(2), 11 s per point with the wavelength of 810 nm in three sessions with an interval of 72 h), G4: low-power laser with low dose + Gluma Desensitizer, G5: low-power laser with high dose + Gluma Desensitizer, G6: Nd:YAG laser (Power Laser™ S...

Journal of Biomedical Optics, 2015

The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary c... more The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. Thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (RMGIC)], LEDTB [photodynamic antimicrobial chemotherapy (PACT) with light-emitting diode associated with toluidine blue solution and RMGIC], and LMB [PACT with laser associated with methylene blue solution and RMGIC]. The participants were submitted to initial clinical and radiographic examinations. Demographic features and biofilm, gingival, and DMFT/DMFS indexes were evaluated, in addition to clinical and radiographic followups at 6 and 12 months after treatments. Carious dentin was collected before and after each treatment, and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopobium rimae, and total bacteria was established by quantitative polymerase chain reaction. No signs of pain or restoration failure were observed. All therapies were effective in reducing the number of microorganisms, except for S. sobrinus. No statistical differences were observed among the protocols used. All therapies may be considered as effective modern approaches to minimal intervention for the management of deep primary caries treatment.

Case Reports in Dentistry, 2015

Among the new technologies developed, low power lasers have enabled new approaches to provide con... more Among the new technologies developed, low power lasers have enabled new approaches to provide conservative treatment. Low power lasers act at cellular level, resulting in reduced pain, modulating inflammation, and improved tissue healing. Clinical application of the low power laser requires specific knowledge concerning laser interaction with biological tissue so that the correct irradiation protocol can be established. The present case report describes the clinical steps involved in an indirect composite resin restoration performed in a 31-year-old patient, in whom low power laser was used for soft tissue biomodulation. Laser therapy was applied with a semiconductor laser 660 nm, spot size of 0.028 cm2, energy density of 35.7 J/cm2, mean power of 100 mW, and energy per point as 1 J, in contact mode, on a total of 2 points (mesial and distal), totaling 2 J of energy. The therapy with low power laser can contribute positively to the success of an indirect restorative treatment.

Lasers in Medical Science, 2015

This study evaluated the progression of enamel erosion after treatment with gels containing sodiu... more This study evaluated the progression of enamel erosion after treatment with gels containing sodium fluoride (NaF; 9047 ppm F) and stannous chloride (SnCl 2 ; 3000 ppm Sn), associated or not with Nd:YAG laser irradiation. Sixty enamel specimens were prepared from bovine incisors and protected by a tape, leaving an exposed surface area of 4× 1 mm. The specimens were immersed in 1 % citric acid (pH= 2.3) for 10 min to create an initial erosion lesion. After, they were randomly divided into six groups: (C) control: gel without active ingredient; (F): NaF gel; (F+Sn): NaF+SnCl 2 gel; (laser): Nd:YAG laser irradiation (0.5 W; 50 mJ; ∼41.66 J/ cm 2 ; 10 Hz; 40 s); (F+laser): NaF gel+Nd:YAG; (F+Sn+ laser): NaF+SnCl 2 gel+Nd:YAG. All gels had pH=4.5 and were applied for 1 min. Laser irradiation was performed after gel application. The specimens were then submitted to a 5-day erosion-remineralization cycling model using 1 % citric acid (pH=2.3), six times per day. Enamel surface loss (SL) was analyzed by optical profilometry in the end of the cycling (in μm). Data were analyzed by one-way ANOVA and Holm-Sidak tests (alpha=0.05). The control and the laser groups presented the highest enamel loss (means±SD=53.52±3.65 and 53.30±2.73, respectively), followed by F+Sn (44.76± 2.83). The groups F (36.76±2.28), F+laser (36.25±3.59), and F+Sn+laser (39.83±4.62) showed the lowest enamel loss, with no significant difference among them (p>0.05). In conclusion, NaF by itself or associated with SnCl 2 and Nd:YAG laser was able to reduce enamel erosion progression. Nd:YAG laser alone did not show a protective effect.

Brazilian Dental Science, 2014

Objective: Although the effects of Er:YAG (erbium:yttrium aluminium garnet) laser on cavity pre... more Objective: Although the effects of Er:YAG (erbium:yttrium aluminium garnet) laser on cavity preparation as well as on dentin bonding to composite have been described in the literature, the longevity of this bond is still unknown. So, this study evaluated the short-term microtensile bond strength to dentin samples after different protocols of surface treatment. Materials and Methods: 60 bovine incisors were cleaned, worn to expose a dentin area and subdivided into groups according to treatment conditions: surface treatment (no irradiation – control group; dentin irradiation with Er:YAG laser 250 mJ/4 Hz; 160 mJ/10 Hz), adhesive system (Clearfil SE Bond - Kuraray; Adper Single Bond 2 - 3M/ESPE), and storage time (24 h; 90 days). After adhesive procedures, a block of Z250 composite resin (3M/ESPE) was built-up on each tooth. The teeth were sectioned to obtain samples for the microtensile bond strength test. Half of the samples were tested 24 h after cutting, and the o...

Brazilian Dental Science, 2012

As pesquisas tem investigado do uso do laser na odontologia

Photodiagnosis and Photodynamic Therapy, 2015

Clinical and Laboratorial Research in Dentistry, 2014

Objective: To quantify, by means of profi lometry, the removal of dental enamel during the use of... more Objective: To quantify, by means of profi lometry, the removal of dental enamel during the use of a microabrasion technique involving the use of hydrochloric acid and manual abrasion with a plastic spatula. Method: Thirty six specimens obtained from human third molars were polished to obtain fl at surfaces and divided into 3 groups (n = 12) according to the different treatments received: A placebo treatment with deionized water as a negative control (CG); microabrasion with 6.6% hydrochloric acid, Opalustre TM (G1); and microabrasion with 6% hydrochloric acid, Whiteness RM TM (G2). The microabrasion was performed in a standardized manner by submitting the specimens to 4 cycles of 10 seconds each and manual abrasion using a plastic spatula (200 g load). The loss of enamel surface was measured after each cycle of treatment by contact profi lometry. Results: Enamel loss was already observed after the fi rst 10 seconds of abrasion with hydrochloric acid in both treated groups (G1 and G2). After 4 abrasions of 10 seconds each, the average fi nal enamel losses in the treated groups were 46.04 µm (G1) and 54.65 µm (G2). In the G1 and G2 groups, a signifi cant increase in enamel wear was detected in each cycle in comparison to the control group (p ≤ 0.05). A signifi cant difference in enamel loss between G1 and G2 was found after 30 and 40 seconds of microabrasion. Relevance: The results of this study provide objective data for safely performing the microabrasion technique on dental enamel using hydrochloric acid and manual abrasion using a plastic spatula. Dental Enamel; Hydrochloric Acid; Enamel Microabrasion. Análise quantitativa da remoção de esmalte dental durante a técnica de microabrasão • Objetivo: Quantifi car, por meio de perfi lometria, a profundidade de esmalte dental removido durante o emprego de uma técnica de microabrasão utilizando-se ácido clorídrico e abrasão manual com espátula plástica. Método: Trinta e seis espécimes obtidos de terceiros molares humanos foram polidos, para obtenção de superfícies planas, e divididos em 3 grupos (n = 12) de acordo com os diferentes tratamentos recebidos: tratamento placebo com água deionizada, como controle negativo (CG); microabrasão com ácido clorídrico a 6.6%, Opalustre TM (G1); e ácido clorídrico a 6%, Whiteness RM TM (G2). A microabrasão foi realizada, de forma padronizada, submetendo os espécimes a 4 ciclos de 10 segundos cada e abrasão manual utilizando-se uma espátula plástica com carga de 200 g. A perda da superfície de esmalte foi medida após cada um dos ciclos de tratamento por meio de perfi lômetro de contato. Resultados: Após os primeiros 10 segundos de abrasão, já foi encontrada perda de esmalte em ambos os grupos tratados (G1 e G2). Nos grupos G1 e G2, a cada ciclo de 10 segundos, foi observado um aumento signifi cativo na perda de esmalte (p ≤ 0.05). Após 4 abrasões de 10 segundos cada, as médias de perda de esmalte nos grupos tratados foram 46.04 µm (G1) e 54.65 µm (G2). Foi encontrada uma diferença signifi cativa entre G1 e G2 com relação à perda de esmalte após 30 e 40 segundos de microabrasão. Relevância: Os resultados deste estudo fornecem referências para a realização do procedimento de microabrasão em esmalte dental com segurança, utilizando-se ácido clorídrico e abrasão manual com espátula plástica. Esmalte Dentário; Ácido Clorídrico; Microabrasão do Esmalte.

Case Reports in Dentistry, 2014

The increasing concern and the search for conservative dental treatments have resulted in the dev... more The increasing concern and the search for conservative dental treatments have resulted in the development of several new technologies. Low and high power lasers can be cited as one of these new technologies. Low power lasers act at cellular level leading to pain reduction, modulation of inflammation, and improvement of tissue healing. High power lasers act by increasing temperature and have the potential to promote microbial reduction and ablation of hard and soft tissues. The clinical application of both low and high power lasers requires specific knowledge concerning laser interaction with biological tissues, so that the correct irradiation protocol can be established. The present case report describes the clinical steps of two metal-ceramic crowns development in a 60-year-old patient. Three different laser wavelengths were applied throughout the treatment with different purposes: Nd:YAG laser (1,064 nm) for dentin decontamination, diode (660 nm) for soft tissue biomodulation, and...

Lasers in Dentistry XIV, 2008

Introduction: New technologies in dental practice, such as laser, have enabled new strategies to ... more Introduction: New technologies in dental practice, such as laser, have enabled new strategies to be established in dental education. The aim of this study was to analyze the difficulties that dental students encountered with performing surgical incisions using Er:YAG laser (LE), and the morphology of these incisions. Material and Methods: Sixteen undergraduate dental students and ten dental professionals (DP) enrolled

Special Care in Dentistry, 2009

This clinical case study reports on dry mouth symptoms in a patient with Sjögren's syndrome (SS) ... more This clinical case study reports on dry mouth symptoms in a patient with Sjögren's syndrome (SS) who was treated with laser phototherapy (LPT). A 60-year-old woman diagnosed with SS was referred to the laboratory for lasers in dentistry to treat her severe xerostomia. A diode laser (780 nm, 3.8 J/cm 2 , 15 mW) was used to irradiate the parotid, submandibular, and sublingual glands, three times per week, for a period of 8 months. The salivary flow rate and xerostomia symptoms were measured before, during, and after LPT. Dry mouth symptoms improved during LPT. After LPT, the parotid salivary gland pain and swelling were no longer present. Treatment with LPT was an effective method to improve the quality of life of this patient with SS.

Photomedicine and Laser Surgery, 2007

Objective: The purpose of this study was to evaluate the tensile bond strength of a self-etching ... more Objective: The purpose of this study was to evaluate the tensile bond strength of a self-etching primer system to enamel and dentin surfaces treated with Er:YAG and Er,Cr:YSGG lasers. Background Data: The recently introduced self-etching primer systems have been shown to adhere better to dental surfaces with thin or no smear layers. Moreover, there have been no previous reports on the bond strength of these adhesives to Er,Cr:YSGG laser-irradiated enamel and dentin, which have been shown to be free of a smear layer. Methods: Thirty samples of enamel and thirty of dentin were divided into three groups. The first group of each substrate served as a control with a standardized bur cut, and the other two groups were conditioned with Er:YAG (350 mJ, 10 Hz, 20 J/cm 2 for enamel; 300 mJ, 6 Hz, 17 J/cm 2 for dentin) and Er,Cr:YSGG laser (125 mJ, 20 Hz, 16 J/cm 2 for both substrates). After the bonding procedure, samples were restored with composite resin, and the tensile bond strength test was performed. Results: The ANOVA two-way analysis and the Tukey test at 5% significance level showed that for enamel and dentin, the bond strength values were statistically higher in Er:YAG-laser treated than in Er,Cr:YSGG-laser treated surfaces (p ‫؍‬ 0.0001). However, bond strength means for both laser-irradiated groups were statistically lower than for the bur cut group (Er:YAG: p ‫؍‬ 0.0281 and Er,Cr:YSGG: p Ͻ 0.0001). SEM observation of laser-irradiated surfaces revealed a roughened aspect and absence of smear layer. Conclusions: The self-etching system adhesion was influenced by the type of erbium laser used, and the bond strength was higher in the Er:YAG-laser irradiated than in the Er,Cr:YSGG-laser irradiated surfaces.

Photomedicine and Laser Surgery, 2009

Background Data and Objective: Herpes is a common infectious disease that is caused by human herp... more Background Data and Objective: Herpes is a common infectious disease that is caused by human herpesviruses. Several treatments have been proposed, but none of them prevent reactivation of the virus. This article describes the use of photodynamic therapy (PDT) as a treatment for herpes lesions, and reports on four cases. Materials and Methods: PDT was used as an adjuvant therapy for the treatment of herpes labialis in four patients. A special type of 0.01% (m/V) of methylene blue solution was applied to the vesicular stage of herpesviral disease and the lesions were irradiated with laser energy (wavelength 660 nm, energy density 120 J/cm 2 , output power of 40 mW, 2 min per point, 4.8 J of energy/point, at four points). After 24 h the patients returned and phototherapy was repeated with the same equipment, this time with 3.8 J/cm 2 and 15 mW, for a total dose of 0.6 J. The same procedure was repeated 72 h and 1 wk later. Results: Treatment with low-level laser therapy can be considered as an option in the treatment of herpes labialis, and decreases the frequency of vesicle recurrence and provides comfort for patients. No significant acute side effects were noted and the lesions healed rapidly. Conclusion: Treatment of herpes labialis with PDT was effective, had no side effects, and when associated with laser phototherapy, accelerated the healing process.

Photomedicine and Laser Surgery, 2005

Objective: The purpose of this study was to assess whether there were alterations in the thyroid ... more Objective: The purpose of this study was to assess whether there were alterations in the thyroid hormone plasma levels under infrared laser irradiation, in the thyroid gland region. Background Data: Studies have demonstrated that infrared laser can cause alterations in thyroid glands. Methods: Sixty-five albino male mice were used and assigned to five groups (n = 13), with differences in the times that they were sacrificed. Irradiation procedures consisted of an infrared diode laser emitting at 780 nm, at 4 J/cm 2 energy density, in contact mode, point manner. Blood was collected before irradiation (group 1), and then at 24 h (group 2), 48 h (group 3) and 72 h (group 4), and 1 week (group 5) after the third irradiation. The collected material was used for clinical analysis to evaluate the T 3 (triiodothyronine) and T 4 (thyroxin) hormones. Five animals were used for light microscopy analysis. Results: A statistically significant hormonal level alteration between the first day and 7 days after the last irradiation was found. Conclusions: It was concluded that low-level laser therapy (LLLT) of the thyroid gland may affect the level of thyroidal hormones. 568 Azevedo et al. were followed during the study. The animals were randomly assigned to five groups (n = 13):

Brazilian dental science, Dec 15, 2017

Brazilian Oral Research, 2013

This study evaluated the effects of the photoactivation source and restorative material on the de... more This study evaluated the effects of the photoactivation source and restorative material on the development of caries-like lesions on human enamel after an in vitro pH challenge. Enamel cavities were prepared in 36 blocks, which were assigned to two groups according to the restorative material: resin-modified glass ionomer (RMGI) and composite resin (CR). Samples were exposed to quartz-tungsten-halogen lamp, argon-ion laser, or light-emitting diode (n = 6). The Knoop microhardness (KHN) values of the top surface of all materials were evaluated. Restored enamel blocks were thermocycled and subjected to 10 demineralization-remineralization cycles at 37°C. KHN analysis of the superficial enamel was performed by four indentations located 100 µm from the restoration margin. The material KHN was not affected by the photoactivation source. No significant difference in KHN was noted between CR and RMGI. The enamel surface around RMGI exhibited a higher KHN (272.8 KHN) than the enamel around CR (93.3 KHN), regardless of the photoactivation source. Enamel demineralization around the dental restoration was not influenced by the photoactivation source. Less enamel demineralization was observed around the RMGI than around the CR restoration.

Lasers in Medical Science, 2010

The objective of this study was to evaluate the influence of Er:YAG laser (λ = 2.94 μm) on microt... more The objective of this study was to evaluate the influence of Er:YAG laser (λ = 2.94 μm) on microtensile bond strength (μTBS) and superficial morphology of bovine dentin bleached with 16% carbamide peroxide. Forty bovine teeth blocks (7 × 3 × 3 mm(3)) were randomly assigned to four groups: G1- bleaching and Er:YAG irradiation with energy density of 25.56 J/cm(2) (focused mode); G2 - bleaching; G3 - no-bleaching and Er:YAG irradiation (25.56 J/cm(2)); G4 - control, non-treated. G1 and G2 were bleached with 16% carbamide peroxide for 6 h during 21 days. Afterwards, all blocks were abraded with 320 to 600-grit abrasive papers to obtain flat standardized dentin surfaces. G1 and G3 were Er:YAG irradiated. Blocks were immediately restored with 4-mm-high composite resin (Adper Single Bond 2, Z-250-3 M/ESPE). After 24 h, the restored blocks (n = 9) were serially sectioned and trimmed to an hour-glass shape of approximately 1 mm(2) at the bonded interface area, and tested in tension in a universal testing machine (1 mm/ min). Failure mode was determined at a magnification of 100× using a stereomicroscope. One block of each group was selected for scanning electron microscope (SEM) analysis. μTBS data was analyzed by two-way ANOVA and Tukey test (α = 0.05). Mean bond strengths (SD) in MPa were: G1- 32.7 (5.9)(A); G2- 31.1 (6.3)(A); G3- 25.2 (8.3)(B); G4- 36.7 (9.9).(A) Groups with different uppercase letters were significantly different from each other (p < .05). Enamel bleaching procedure did not affect μTBS values for dentin adhesion. Er:YAG laser irradiation with 25.56 J/cm(2) prior to adhesive procedure of bleached teeth did not affect μTBS at dentin and promoted a dentin surface with no smear layer and opened dentin tubules observed under SEM. On the other hand, Er:YAG laser irradiation prior to adhesive procedure of non-bleached surface impaired μTBS compared to the control group.

International Journal of Adhesion and Adhesives, 2012

Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three... more Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three adhesive systems (AS) on dentin surfaces treated with Er:YAG laser using two irradiation parameters. Study Design: Occlusal flat dentin surfaces of 36 human third molars were assigned into nine groups (n ¼ 4) according to the following ASs: one bottle etch&rinse Single Bond Plus (3M ESPE), two-step Clearfil Protect Bond (Kuraray), and all-in-one S 3 Bond (Kuraray) self-etching, which were labeled with rhodamine B or fluorescein isothiocyanate-dextran and were applied to dentin surfaces that were irradiated with Er:YAG laser at either 120 (38.7 J/cm 2) or 200 mJ/pulse (64.5 J/cm 2), or were applied to untreated dentin surfaces (control group). The ASs were light-activated following MI and the bonded surfaces were restored with resin composite Z250 (3M ESPE). After 24 hours of storage in vegetable oil, the restored teeth were vertically, serially sectioned into 1-mm thick slabs, which had the adhesive interfaces analyzed with confocal laser microscope (CLSM-LSM 510 Meta). CLSM images were recorded in the fluorescent mode from three different regions along each bonded interface. Results: Non-uniform HL was created on laser-irradiated dentin surfaces regardless of laser irradiation protocol for all AS, while regular and uniform HL was observed in the control groups. ''Stretch mark''-like red lines were found within the HL as a result of resin infiltration into dentin microfissures, which were predominantly observed in 200 mJ/pulse groups regardless of AS. Poor resin infiltration into peritubular dentin was observed in most regions of adhesive interfaces created by all ASs on laser-irradiated dentin, resulting in thin resin tags with neither funnelshaped morphology nor lateral resin projections. Conclusion: Laser irradiation of dentin surfaces at 120 or 200 mJ/pulse resulted in morphological changes in HL and resin tags for all ASs evaluated in the study.

INTRODUCTION The purpose of a desensitizing agent is a permanent coating or filling of dentin sur... more INTRODUCTION The purpose of a desensitizing agent is a permanent coating or filling of dentin surface. Morphological analysis in vitro of this treated surface is essential to understand the interaction between desensitizing agent and hypersensitive dentin. The aim was to evaluate the morphology of four dentin surface treated with desensitizing agents. METHODS This was an in vitro laboratory study, where fifteen specimens from extracted human premolars were obtained. The enamel was removed to expose the dentin surface, polished with silicon carbide abrasive papers and etched with 6% citric acid for 2 min.The specimens were randomly divided into 5 groups: G1 - without treatment (control) (C), G2 - fluoride varnish (FV), G3 - potassium oxalate (PO), G4 - 2-step self-etching adhesive system (AS), G5 - diode laser (DL). The specimens were cleaved in the lingual buccaldirection, prepared for analysis by Scanning Electron Microscope and the surface and interior of the dentinal tubules were...

Photodiagnosis and photodynamic therapy, Jan 28, 2017

Chronic gingivostomatitis in dogs is an inflammatory syndrome of the oral cavity, which treatment... more Chronic gingivostomatitis in dogs is an inflammatory syndrome of the oral cavity, which treatment and control of concomitant periodontitis allow healing in most of the cases. In the presence of recurrent lesions, invasive methods are necessary to treat lesions and pain. As a conservative adjuvant method, photobiomodulation (PBM) with low power laser is able to promote reduction of tissue pain and tissue inflammation besides increasing vascularization and healing, restoring the normal function of the irradiated organ in a shorter time. In veterinary medicine, there is no standardization of technique for its use in oral tissue for treating gingivostomatitis in dogs. In the present case, a dog was submitted to aPDT (7.2J/point, 3min/point, 180J/cm(2)) and PBM (1.6J/point, 40sec/point, 25J/cm(2)), using a semiconductor diode laser, with wavelength of 660nm, spot size of ​​0.04cm(2) and output power of 40mW. The established protocol proved to be effective as coadjutant treatment for chro...

Lasers in medical science, 2017

This randomized and longitudinal in vivo study aimed to assess different protocols for the treatm... more This randomized and longitudinal in vivo study aimed to assess different protocols for the treatment of dentin hypersensitivity with low-power laser (with different doses), high-power laser, and a desensitizing agent, for a period of 12 and 18 months. The lesions from 32 patients (117 lesions), who were submitted to the inclusion and exclusion criteria, were divided into nine groups (n = 13): G1: Gluma Desensitizer (Heraeus Kulzer), G2: low-power laser with low dose (three points of irradiation in vestibular portion and an apical point 30 mW, 10 J/cm(2), 9 s per point with the wavelength of 810 nm, with three sessions with an interval of 72 h), G3: low-power laser with high dose (one point in the cervical area, and one apical point 100 mW, 40 J/cm(2), 11 s per point with the wavelength of 810 nm in three sessions with an interval of 72 h), G4: low-power laser with low dose + Gluma Desensitizer, G5: low-power laser with high dose + Gluma Desensitizer, G6: Nd:YAG laser (Power Laser™ S...

Journal of Biomedical Optics, 2015

The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary c... more The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. Thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (RMGIC)], LEDTB [photodynamic antimicrobial chemotherapy (PACT) with light-emitting diode associated with toluidine blue solution and RMGIC], and LMB [PACT with laser associated with methylene blue solution and RMGIC]. The participants were submitted to initial clinical and radiographic examinations. Demographic features and biofilm, gingival, and DMFT/DMFS indexes were evaluated, in addition to clinical and radiographic followups at 6 and 12 months after treatments. Carious dentin was collected before and after each treatment, and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopobium rimae, and total bacteria was established by quantitative polymerase chain reaction. No signs of pain or restoration failure were observed. All therapies were effective in reducing the number of microorganisms, except for S. sobrinus. No statistical differences were observed among the protocols used. All therapies may be considered as effective modern approaches to minimal intervention for the management of deep primary caries treatment.

Case Reports in Dentistry, 2015

Among the new technologies developed, low power lasers have enabled new approaches to provide con... more Among the new technologies developed, low power lasers have enabled new approaches to provide conservative treatment. Low power lasers act at cellular level, resulting in reduced pain, modulating inflammation, and improved tissue healing. Clinical application of the low power laser requires specific knowledge concerning laser interaction with biological tissue so that the correct irradiation protocol can be established. The present case report describes the clinical steps involved in an indirect composite resin restoration performed in a 31-year-old patient, in whom low power laser was used for soft tissue biomodulation. Laser therapy was applied with a semiconductor laser 660 nm, spot size of 0.028 cm2, energy density of 35.7 J/cm2, mean power of 100 mW, and energy per point as 1 J, in contact mode, on a total of 2 points (mesial and distal), totaling 2 J of energy. The therapy with low power laser can contribute positively to the success of an indirect restorative treatment.

Lasers in Medical Science, 2015

This study evaluated the progression of enamel erosion after treatment with gels containing sodiu... more This study evaluated the progression of enamel erosion after treatment with gels containing sodium fluoride (NaF; 9047 ppm F) and stannous chloride (SnCl 2 ; 3000 ppm Sn), associated or not with Nd:YAG laser irradiation. Sixty enamel specimens were prepared from bovine incisors and protected by a tape, leaving an exposed surface area of 4× 1 mm. The specimens were immersed in 1 % citric acid (pH= 2.3) for 10 min to create an initial erosion lesion. After, they were randomly divided into six groups: (C) control: gel without active ingredient; (F): NaF gel; (F+Sn): NaF+SnCl 2 gel; (laser): Nd:YAG laser irradiation (0.5 W; 50 mJ; ∼41.66 J/ cm 2 ; 10 Hz; 40 s); (F+laser): NaF gel+Nd:YAG; (F+Sn+ laser): NaF+SnCl 2 gel+Nd:YAG. All gels had pH=4.5 and were applied for 1 min. Laser irradiation was performed after gel application. The specimens were then submitted to a 5-day erosion-remineralization cycling model using 1 % citric acid (pH=2.3), six times per day. Enamel surface loss (SL) was analyzed by optical profilometry in the end of the cycling (in μm). Data were analyzed by one-way ANOVA and Holm-Sidak tests (alpha=0.05). The control and the laser groups presented the highest enamel loss (means±SD=53.52±3.65 and 53.30±2.73, respectively), followed by F+Sn (44.76± 2.83). The groups F (36.76±2.28), F+laser (36.25±3.59), and F+Sn+laser (39.83±4.62) showed the lowest enamel loss, with no significant difference among them (p>0.05). In conclusion, NaF by itself or associated with SnCl 2 and Nd:YAG laser was able to reduce enamel erosion progression. Nd:YAG laser alone did not show a protective effect.

Brazilian Dental Science, 2014

Objective: Although the effects of Er:YAG (erbium:yttrium aluminium garnet) laser on cavity pre... more Objective: Although the effects of Er:YAG (erbium:yttrium aluminium garnet) laser on cavity preparation as well as on dentin bonding to composite have been described in the literature, the longevity of this bond is still unknown. So, this study evaluated the short-term microtensile bond strength to dentin samples after different protocols of surface treatment. Materials and Methods: 60 bovine incisors were cleaned, worn to expose a dentin area and subdivided into groups according to treatment conditions: surface treatment (no irradiation – control group; dentin irradiation with Er:YAG laser 250 mJ/4 Hz; 160 mJ/10 Hz), adhesive system (Clearfil SE Bond - Kuraray; Adper Single Bond 2 - 3M/ESPE), and storage time (24 h; 90 days). After adhesive procedures, a block of Z250 composite resin (3M/ESPE) was built-up on each tooth. The teeth were sectioned to obtain samples for the microtensile bond strength test. Half of the samples were tested 24 h after cutting, and the o...

Brazilian Dental Science, 2012

As pesquisas tem investigado do uso do laser na odontologia

Photodiagnosis and Photodynamic Therapy, 2015

Clinical and Laboratorial Research in Dentistry, 2014

Objective: To quantify, by means of profi lometry, the removal of dental enamel during the use of... more Objective: To quantify, by means of profi lometry, the removal of dental enamel during the use of a microabrasion technique involving the use of hydrochloric acid and manual abrasion with a plastic spatula. Method: Thirty six specimens obtained from human third molars were polished to obtain fl at surfaces and divided into 3 groups (n = 12) according to the different treatments received: A placebo treatment with deionized water as a negative control (CG); microabrasion with 6.6% hydrochloric acid, Opalustre TM (G1); and microabrasion with 6% hydrochloric acid, Whiteness RM TM (G2). The microabrasion was performed in a standardized manner by submitting the specimens to 4 cycles of 10 seconds each and manual abrasion using a plastic spatula (200 g load). The loss of enamel surface was measured after each cycle of treatment by contact profi lometry. Results: Enamel loss was already observed after the fi rst 10 seconds of abrasion with hydrochloric acid in both treated groups (G1 and G2). After 4 abrasions of 10 seconds each, the average fi nal enamel losses in the treated groups were 46.04 µm (G1) and 54.65 µm (G2). In the G1 and G2 groups, a signifi cant increase in enamel wear was detected in each cycle in comparison to the control group (p ≤ 0.05). A signifi cant difference in enamel loss between G1 and G2 was found after 30 and 40 seconds of microabrasion. Relevance: The results of this study provide objective data for safely performing the microabrasion technique on dental enamel using hydrochloric acid and manual abrasion using a plastic spatula. Dental Enamel; Hydrochloric Acid; Enamel Microabrasion. Análise quantitativa da remoção de esmalte dental durante a técnica de microabrasão • Objetivo: Quantifi car, por meio de perfi lometria, a profundidade de esmalte dental removido durante o emprego de uma técnica de microabrasão utilizando-se ácido clorídrico e abrasão manual com espátula plástica. Método: Trinta e seis espécimes obtidos de terceiros molares humanos foram polidos, para obtenção de superfícies planas, e divididos em 3 grupos (n = 12) de acordo com os diferentes tratamentos recebidos: tratamento placebo com água deionizada, como controle negativo (CG); microabrasão com ácido clorídrico a 6.6%, Opalustre TM (G1); e ácido clorídrico a 6%, Whiteness RM TM (G2). A microabrasão foi realizada, de forma padronizada, submetendo os espécimes a 4 ciclos de 10 segundos cada e abrasão manual utilizando-se uma espátula plástica com carga de 200 g. A perda da superfície de esmalte foi medida após cada um dos ciclos de tratamento por meio de perfi lômetro de contato. Resultados: Após os primeiros 10 segundos de abrasão, já foi encontrada perda de esmalte em ambos os grupos tratados (G1 e G2). Nos grupos G1 e G2, a cada ciclo de 10 segundos, foi observado um aumento signifi cativo na perda de esmalte (p ≤ 0.05). Após 4 abrasões de 10 segundos cada, as médias de perda de esmalte nos grupos tratados foram 46.04 µm (G1) e 54.65 µm (G2). Foi encontrada uma diferença signifi cativa entre G1 e G2 com relação à perda de esmalte após 30 e 40 segundos de microabrasão. Relevância: Os resultados deste estudo fornecem referências para a realização do procedimento de microabrasão em esmalte dental com segurança, utilizando-se ácido clorídrico e abrasão manual com espátula plástica. Esmalte Dentário; Ácido Clorídrico; Microabrasão do Esmalte.

Case Reports in Dentistry, 2014

The increasing concern and the search for conservative dental treatments have resulted in the dev... more The increasing concern and the search for conservative dental treatments have resulted in the development of several new technologies. Low and high power lasers can be cited as one of these new technologies. Low power lasers act at cellular level leading to pain reduction, modulation of inflammation, and improvement of tissue healing. High power lasers act by increasing temperature and have the potential to promote microbial reduction and ablation of hard and soft tissues. The clinical application of both low and high power lasers requires specific knowledge concerning laser interaction with biological tissues, so that the correct irradiation protocol can be established. The present case report describes the clinical steps of two metal-ceramic crowns development in a 60-year-old patient. Three different laser wavelengths were applied throughout the treatment with different purposes: Nd:YAG laser (1,064 nm) for dentin decontamination, diode (660 nm) for soft tissue biomodulation, and...

Lasers in Dentistry XIV, 2008

Introduction: New technologies in dental practice, such as laser, have enabled new strategies to ... more Introduction: New technologies in dental practice, such as laser, have enabled new strategies to be established in dental education. The aim of this study was to analyze the difficulties that dental students encountered with performing surgical incisions using Er:YAG laser (LE), and the morphology of these incisions. Material and Methods: Sixteen undergraduate dental students and ten dental professionals (DP) enrolled

Special Care in Dentistry, 2009

This clinical case study reports on dry mouth symptoms in a patient with Sjögren's syndrome (SS) ... more This clinical case study reports on dry mouth symptoms in a patient with Sjögren's syndrome (SS) who was treated with laser phototherapy (LPT). A 60-year-old woman diagnosed with SS was referred to the laboratory for lasers in dentistry to treat her severe xerostomia. A diode laser (780 nm, 3.8 J/cm 2 , 15 mW) was used to irradiate the parotid, submandibular, and sublingual glands, three times per week, for a period of 8 months. The salivary flow rate and xerostomia symptoms were measured before, during, and after LPT. Dry mouth symptoms improved during LPT. After LPT, the parotid salivary gland pain and swelling were no longer present. Treatment with LPT was an effective method to improve the quality of life of this patient with SS.

Photomedicine and Laser Surgery, 2007

Objective: The purpose of this study was to evaluate the tensile bond strength of a self-etching ... more Objective: The purpose of this study was to evaluate the tensile bond strength of a self-etching primer system to enamel and dentin surfaces treated with Er:YAG and Er,Cr:YSGG lasers. Background Data: The recently introduced self-etching primer systems have been shown to adhere better to dental surfaces with thin or no smear layers. Moreover, there have been no previous reports on the bond strength of these adhesives to Er,Cr:YSGG laser-irradiated enamel and dentin, which have been shown to be free of a smear layer. Methods: Thirty samples of enamel and thirty of dentin were divided into three groups. The first group of each substrate served as a control with a standardized bur cut, and the other two groups were conditioned with Er:YAG (350 mJ, 10 Hz, 20 J/cm 2 for enamel; 300 mJ, 6 Hz, 17 J/cm 2 for dentin) and Er,Cr:YSGG laser (125 mJ, 20 Hz, 16 J/cm 2 for both substrates). After the bonding procedure, samples were restored with composite resin, and the tensile bond strength test was performed. Results: The ANOVA two-way analysis and the Tukey test at 5% significance level showed that for enamel and dentin, the bond strength values were statistically higher in Er:YAG-laser treated than in Er,Cr:YSGG-laser treated surfaces (p ‫؍‬ 0.0001). However, bond strength means for both laser-irradiated groups were statistically lower than for the bur cut group (Er:YAG: p ‫؍‬ 0.0281 and Er,Cr:YSGG: p Ͻ 0.0001). SEM observation of laser-irradiated surfaces revealed a roughened aspect and absence of smear layer. Conclusions: The self-etching system adhesion was influenced by the type of erbium laser used, and the bond strength was higher in the Er:YAG-laser irradiated than in the Er,Cr:YSGG-laser irradiated surfaces.

Photomedicine and Laser Surgery, 2009

Background Data and Objective: Herpes is a common infectious disease that is caused by human herp... more Background Data and Objective: Herpes is a common infectious disease that is caused by human herpesviruses. Several treatments have been proposed, but none of them prevent reactivation of the virus. This article describes the use of photodynamic therapy (PDT) as a treatment for herpes lesions, and reports on four cases. Materials and Methods: PDT was used as an adjuvant therapy for the treatment of herpes labialis in four patients. A special type of 0.01% (m/V) of methylene blue solution was applied to the vesicular stage of herpesviral disease and the lesions were irradiated with laser energy (wavelength 660 nm, energy density 120 J/cm 2 , output power of 40 mW, 2 min per point, 4.8 J of energy/point, at four points). After 24 h the patients returned and phototherapy was repeated with the same equipment, this time with 3.8 J/cm 2 and 15 mW, for a total dose of 0.6 J. The same procedure was repeated 72 h and 1 wk later. Results: Treatment with low-level laser therapy can be considered as an option in the treatment of herpes labialis, and decreases the frequency of vesicle recurrence and provides comfort for patients. No significant acute side effects were noted and the lesions healed rapidly. Conclusion: Treatment of herpes labialis with PDT was effective, had no side effects, and when associated with laser phototherapy, accelerated the healing process.

Photomedicine and Laser Surgery, 2005

Objective: The purpose of this study was to assess whether there were alterations in the thyroid ... more Objective: The purpose of this study was to assess whether there were alterations in the thyroid hormone plasma levels under infrared laser irradiation, in the thyroid gland region. Background Data: Studies have demonstrated that infrared laser can cause alterations in thyroid glands. Methods: Sixty-five albino male mice were used and assigned to five groups (n = 13), with differences in the times that they were sacrificed. Irradiation procedures consisted of an infrared diode laser emitting at 780 nm, at 4 J/cm 2 energy density, in contact mode, point manner. Blood was collected before irradiation (group 1), and then at 24 h (group 2), 48 h (group 3) and 72 h (group 4), and 1 week (group 5) after the third irradiation. The collected material was used for clinical analysis to evaluate the T 3 (triiodothyronine) and T 4 (thyroxin) hormones. Five animals were used for light microscopy analysis. Results: A statistically significant hormonal level alteration between the first day and 7 days after the last irradiation was found. Conclusions: It was concluded that low-level laser therapy (LLLT) of the thyroid gland may affect the level of thyroidal hormones. 568 Azevedo et al. were followed during the study. The animals were randomly assigned to five groups (n = 13):