Arash Pooladi - Academia.edu (original) (raw)

Papers by Arash Pooladi

In this investigation Murine Embryonic Stem (ES) cells were differentiated into endothelial cells... more In this investigation Murine Embryonic Stem (ES) cells were differentiated into endothelial cells. Materials and Methods: Murine ES cells (CCE cell line) exposed to Alpha-MEM medium containing 10% FBS for 4 days. Then obtained Flk-1 (Flk-1: Vascular Endothelial Growth Factor Receptor 2) Positive cells were cultuted in Endothelial Growth Medium-2 (EGM-2) until the last day of experiment. Differentiated cells were evaluated by immunocytochemistry, RT-PCR and Tube Formation Assays. Results: When the ES cells cultured in collagen coated dishes containing Alpha-MEM & FBS, Flk-1 positive cells were obtained. After transfering Flk-1 positive cells into fibronectin coated dishes containing EGM2, the cells were assumed a relatively uniform endothelial cell morphology and could be propagated and expanded. Immunocytochemical and RT-PCR analysis of differentiated cells showed that they take up acetylated low-density lipoprotein (LDL), express Flk-1, CD31 and bind the BS-l lectin. When placed in Matrigel, these Murine ES cell-derived endothelial cells formed capillary-like structures characteristic of endothelial cells Conclusion: ES cell-derived endothelial cells provide a novel means to examine the mechanisms of endothelial cell development, and may open up new therapeutic strategies.

Educational Research in Medical Sciences, 2013

Introduction: It is necessary to analyze the limitations and essentials of the medical students&#... more Introduction: It is necessary to analyze the limitations and essentials of the medical students' learning compatible with their future working environment in order to control the quality of the education. This study was designed to investigate the basics of learning and to prepare educational logbooks for all clinical departments at Kurdistan University of Medical Sciences in line with promotion of educational and students' assessment. Methods: This study was carried out in three phases. In the first phase, content was confirmed according to the expert views. In the second phase, materials were analyzed in terms of being core and non-core. In the third phase, prioritized subjects were summarized and locations selected for teaching each skill, rotation style, regulations and responsibilities of the students, assessment method and group's expectations from students were determined. The data were analyzed by analytic hierarchy process (AHP), comparison of the content of mat...

Sarem Journal of Reproductive Medicine, 2019

Endometriomas as a possible cause of reduced ovarian reserve in women with endometriosis [2] High... more Endometriomas as a possible cause of reduced ovarian reserve in women with endometriosis [2] High prevalence of endometriosis in infertile women with normal ovulation and normospermic partners [3] Laparoscopic colorectal resections with transvaginal specimen extraction for severe endometriosis [4] SEndometriosis and infertility: A committee opinion [5] Effect of endometriosis on IVF/ICSI outcome: Stage III/ IV endometriosis worsens cumulative pregnancy and live-born rates [6] Possible effects of endometriosis-related immune events on reproductive function [7] Endometriosis and infertility [8] Treatment of endometriosis as a priority before art [9] Efficacy analysis of an experience on development of the combination therapy protocol for endometriosis management [10] Endometriosis management; A survey on medical & laparoscopic treatment [11] The impact of endometrioma on IVF/ICSI outcomes: A systematic review and meta-analysis [12] Effects of ovarian endometrioma on embryo quality [13] Evidence linked treatment for endometriosis-associated infertility [14] Assessment of oocyte quality in polycystic ovarian syndrome and endometriosis by spindle imaging and reactive oxygen species levels in follicular fluid and its relationship with IVF-ET outcome Aims The women with infertility caused by endometriosis, need to use assisted reproductive technology after their treatment. The objective of this study was to evaluate fertility rate using ICSI in women with treated endometriosis compared to healthy women. Materials & Methods In this retrospective quasi-experimental study, endometriotic women (n=150) who were treated by endometriosis treatment protocol of Sarem Women's hospital were selected and their fertility rate was compared with that of normal women (n=150) who referred Sarem Women's hospital for ICSI due to obstructive azoospermia of their husbands. The data were analyzed by Spearman rank correlation coefficient, Mann-Whitney U, Fisher's exact and Chi-square tests using SPSS 22 software. Findings The mature oocytes number of case group was significantly lower than control group (p=0.036); however, the fertility rate did not show significant differences between groups (p=0.397). There was a reverse significant correlation between the grade of endometriosis with the total oocyte and mature oocyte counts in endometriotic patients after treatment (p=0.04 and p=0.016, respectively). Conclusion The fertility rate using ICSI method in women after endometriosis treatment is not different from healthy women.

Academic Medicine, 2009

Background and purpose: Problem-based learning (PBL) is a term used within education for a range ... more Background and purpose: Problem-based learning (PBL) is a term used within education for a range of teaching approaches that encourage students to learn through the structured exploration of a problem. Histology comes early in the curriculum and the medical students seem unable to see the value of the content, they don't appear to be motivated to learn the content. This project used PBL to help the students make the connection between the content and clinical aspects. Methods: Thirty six undergraduate medical students, 22 female and 14 male, enrolled in the histology course during the spring s emester of 2008. A survey which collected information relative to gender, course load, and workload and study time was used. The subjects were accessory glands of digestive system histology. The course is designed into four units: tree units of salivary glands, pancreas and gall bladder histology, were presented in a traditional lecture format; the fourth unit, liver was presented in a pro...

Sarem Journal of Reproductive Medicine, 2019

N. Evaluation the success rate of treatment failure in patients with unexplained repeated IVF tre... more N. Evaluation the success rate of treatment failure in patients with unexplained repeated IVF treatments immunization with lymphocytes (LIT) methods.

Sarem Journal of Reproductive Medicine, 2017

The histological features of ... [22] Splenosis: The autotransplantation of splenic tissue follow... more The histological features of ... [22] Splenosis: The autotransplantation of splenic tissue following injury to the spleen; report of two cases and review of the ... [23] A cause of massive gastrointestinal ... [24] Splenosis presenting as occult gastrointestinal ... [25] Splenosis: Ultrasound and CT findings in a case complicated by an intraperitoneal implant traumatic ... [26] Traumatic rupture of splenic tissue 13 years after splenectomy. A case ... [27] Pelvic splenosis mimicking endometriosis, causing low ... [28] Scintigraphic detection of splenosis causing ureteral compression and ... [29] Idiopathic thrombocytopenic purpura occurring in a subject previously splenectomized for traumatic splenic ... [30] Splenic sequestration of 99mTc labeled, heat treated red blood ... [31] A case report of abdominal splenosis-a practical mini-review for a ... [32] Splenosis; Review and report of subcutaneous splenic ... [33] Splenogonadal ... [34] Right splenorenal fusion with ... [35] Female splenic-gonadal fusion of the discontinuous ... [36] Thoracic splenosis: A diagnosis by history ... [37] Fine needle aspiration of thoracic splenosis ... [38] Parenchymal thoracic splenosis: History and nuclear imaging without invasive procedures may provide ... [39] Intrathoracic splenosis: Evaluation by 99mTc-labelled heat-denatured erythrocyte ... [40] MR features of abdominal ... [41] Clinical studies with spleen-specific radiolabeled ... [42] Scintigraphic detection of splenosis: Superiority of tomographic selective spleen ... [43] The born-again spleen. Return of splenic function after splenectomy for ... [44] Minimally invasive surgery in the treatment of ... Patient Information Spontaneous splenic transplantation and implant in in abdominal and pelvic regions is splenosis, which occurs after splenectomy followed by trauma and surgery. The clinical significance of splenic implant and the requirement for its removal are debatable. The cases of splenic implants in the pelvic zone have rarely been reported. Generally, these patients have no clinical features and are usually discovered by chance. In this report, a 36-year-old woman with the history of splenectomy after an accident at the age of 4 was introduced with Dysmenorrhea and infertility; the primary diagnosis was endometriosis, but pathology results reported the splenosis. Whole tissues and implants of spleen around the ovary and choledosac were removed; following this, the patient's pain was reduced and the overall health improved. Conclusion Splenosis can emerge as a benign condition in the abdomen and pelvis, and can show itself as a spread of tissue implants in the pelvic cavity. It can, however, easily be mistaken for endometriosis in imaging.

Sarem Journal of Reproductive Medicine, 2016

Predisposition to cancer and ... [8] Chromosomal radiosensitivity: A study of the chromosomal G(2... more Predisposition to cancer and ... [8] Chromosomal radiosensitivity: A study of the chromosomal G(2) assay in human blood lymphocytes indicating significant ... [9] The G2 ... [10] Is chromosome radiosensitivity and apoptotic response to irradiation correlated with cancer ... [11] Lymphocyte radiosensitivity in BRCA1 and BRCA2 mutation carriers and implications for breast cancer ... [12] Chromosomal radiosensitivity in breast cancer patients with a known or putative genetic ... [13] Increased chromosomal radiosensitivity in breast cancer patients: A comparison of two ... [14] Relationship between in vitro chromosomal radiosensitivity of peripheral ... [15] Heritability of cellular radiosensitivity ... [16] Nucleotide Excision Repair Syndromes ... [17] Xeroderma pigmentosum: A human disease in ... [18] Cytogenetic status of ataxia ... [19] Enhanced chromatid damage ... [20] Radiationinduced micronucleus induction in lymphocytes identifies a ... [21] Genotoxic effects of radiotherapy and ... [22] Chromosomes, cancer and ... [23] Chromosomal radiosensitivity in G2-phase lymphocytes identifies breast cancer ... [24] Increased G2 chromosomal radiosensitivity in cancer patients ... [25] Deficient DNA repair capacity, apredisposing ... [26] Genetic predisposition in ... [27] Evaluating the effects of genetic variants ... [28] G2 checkpoint control and G2 chromosomal radiosensitivity ... [29] DNA repair capacity as a possible biomarker ... [30] Chromosomal radiosensitivity as a ... [31] Validation of the cell cycle G(2) ... [32] Chromosomal radiosensitivity in ... [33] G2-phase chromosomal radiosensitivity ... [34] Spontaneous and radiation-induced ... [35] Chromosomal radiosensitivity in head and neck cancer patients: Evidence for ... Aims The use of non-invasive laboratory tests based on detection of biomarkers in the blood samples is a good strategy for early diagnosis of breast cancer. The lymphocyte radiosensitivity assessment can be a valuable method to diagnose breast cancer. The objective of this study was to investigate the radiosensitivity indices in sporadic breast cancer among Iranian women and to evaluate the potential of those indices for clinical use in early diagnosis of breast cancer and assessing its susceptibility. Materials & Methods The present study is a case-control that was conducted on 32 patients with sporadic breast cancer (patient group) and 30 healthy individuals (control group). The obtained blood samples of both groups were exposed to gamma-irradiation (0.4 Gy) and the level of chromosome breakage was determined based on the G2 chromosome breakage assay protocol. In the metaphase lymphocytes, the percentage of the abnormal cells was calculated as the radiosensitivity index for comparing the two groups. Data were analyzed by SPSS 19 software using student t-test, paired t-test, chi-square and Fisher exact test. Findings There was a significant difference in the percentage of the index of the abnormal cells after irradiation between two groups (p=0.001). The area under the curve (AUC) of the percentage of the abnormal cells and odds ratio (OR) were found as 0.725 with 3.818, respectively. The frequency of increased radiosensitivity based on this index (at 61% cutoff point), was 65.6% in the patients and about 33% in the control group. Conclusion The increased level of chromosome breakage following irradiation with gamma rays can be used as an early diagnostic biomarker of breast cancer or a possible indicator for breast cancer susceptibility.

Sarem Journal of Reproductive Medicine, 2017

Prospective randomized double-blind trial of 3 versus ... [19] Pre and post operative medical the... more Prospective randomized double-blind trial of 3 versus ... [19] Pre and post operative medical therapy ... [20] Revised American Society for Reproductive Medicine classification ... [21] Laparoscopy versus laparotomy in conservative ... [22] Laparoscopic surgery for pelvic pain associated with ... [23] Prospective, randomized, double-blind, controlled trial of laser laparoscopy in the ... [24] A prospective, randomized study comparing laparoscopic ... [25] Randomized clinical trial of two laparoscopic treatments of ... [26] Laparoscopic surgery for the management of ovarian ... [27] Hormone therapy for endometriosis and surgical ... [28] Laparoscopic excision of endometriosis: A randomized ... [29] The impact of treatment on the natural history ... [30] Subcutaneous depot medroxyprogesterone acetate versus leuprolide acetate in ... [31] Randomized trial of leuprolide versus continuous oral contraceptives in the treatment of endometriosis-associated ... [32] The impact of preoperative gonadotropin-releasing ... [33] Use of nafarelin versus placebo after reductive laparoscopic ... [34] ESHRE guideline: Management of women with ... [35] Long-term use of gonadotropin-releasing hormone analogs and hormone replacement therapy in the management of endometriosis ... Aims Laparoscopy is accepted as a golden standard method in treatment of endometriosis. The main objective of this study was to evaluate the effects of endometriosis management protocol using a combined drug-surgery treatment in various stages. Materials & Methods This non-experimental and interventional study was conducted on 47 patients with confirmed endometriosis in Sarem women's hospital (Tehran, Iran) in 2011-2012. The patients were divided into 4 groups according to the endometriosis stages of I to IV. Laparoscopy surgery (electro-coagulation and the adhesions removing) was performed for all patients, and then they treated for monthly injection of GnRH analogues. The second and third laparoscopy procedures were performed after 3 and 6 months for relevant groups, respectively. Findings In the stage-I endometriosis group 9 (90%) patients showed complete recovery after 3 months of treatment and 1 remained case showed complete recovery after 6 months. For stage-II and stage-III groups 16 (72.7%) and 5 (50%) cases showed complete recovery after 6 months of the treatment, respectively. The remained patients of stages II and III treated with GnRH for an additional 3 months and showed complete recovery (excluding one case) after 9 months. In Stage-IV, 2 (40%) cases showed complete recovery and 3 out of 5 patients were candidate to laparotomy. Conclusion The patients with high stages of endometriosis require more invasive and longer treatments. The success of combined drug-surgery treatment for patients in stage-I endometriosis is possible in the course of 3 months. It requires 6 months for patients in stage-II and between 6 to 9 months for patients in stage-III for complete recovery.

Behavior Modification, 2006

The Preferred Items and Experiences Questionnaire (PIEQ) is a new instrument to assess reinforcer... more The Preferred Items and Experiences Questionnaire (PIEQ) is a new instrument to assess reinforcer preferences in adolescents and adults. Research was conducted with college and high school students to develop the PIEQ, to examine its reliability with test-retest and internal consistency methods, and to test its validity. This research provides support for the PIEQ’s reliability and validity.

Sarem Journal of Reproductive Medicine, 2019

Estimating the causes of 4 million neonatal deaths in the year 2000 [2] The role of prenatal care... more Estimating the causes of 4 million neonatal deaths in the year 2000 [2] The role of prenatal care in recurrent preterm birth [3] The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity [4] Preterm delivery in women with pregestational diabetes mellitus or chronic hypertension relative to women with uncomplicated pregnancies [5] Investigating socioeconomic disparities in preterm birth: Evidence for selective study participation and selection bias [6] Socioeconomic disparities in preterm birth: causal pathways and mechanisms [7] Risk Factors for Preterm Birth and Small-for-gestational-age Births among Canadian Women [8] The preterm prediction study: a clinical risk assessment system [9] Paternal race is a risk factor for preterm birth [10] Risk factors for spontaneous preterm birth among aboriginal and non-aboriginal women in Manitoba [11] Prepregnancy body mass index is an independent risk factor for gestational hypertension, gestational diabetes, preterm labor, and small-and large-for-gestational-age infants [12] Urogenital Infections as a Risk Factor for Preterm Labor: A Hospital-Based Case-Control Study [13] Manual of high risk pregnancy and delivery [14] Prevalence of preterm delivery and its related factors in females referring to Shohada Tajrish Hospital [15] Effect of parity and advanced maternal age on obstetric outcome [16] Risk factors associated with preterm (< 37+ 0 weeks) and early preterm birth (< 32+ 0 weeks): Univariate and multivariate analysis of 106 345 singleton births from the 1994 statewide perinatal survey of Bavaria [17] Maternal factors associated to prematurity [18] Abortion and the risk of subsequent preterm birth: a systematic review with meta-analyses [19] Prevalence and maternal risk factors of preterm laboring in Qom, 2007 [20] Prevalence of preterm labor and it's complications in neonates of women with preeclampsia-eclampsia in Emam Reza Hospital [21] Survey of related factors in prematurity birth [22] Gestational diabetes mellitus and lesser degrees of pregnancy hyperglycemia: Association with increased risk of spontaneous preterm birth [23] The association between decreased amniotic fluid volume and treatment with nonsteroidal anti-inflammatory agents for preterm labor [24] Amniotic fluid abnormalities [25] Risks of preterm delivery and association with maternal age, birth order, and fetal gender Aims Preterm labor is the most common cause of mortality among neonates. The risk of spontaneous preterm labor is increased due to various maternal factors. Therefore, the objective of this study was to investigate the maternal factors involved in preterm labor. Materials & Methods This cross-sectional study as a descriptive-analytic and retrospective study was conducted on 2432 women referred to Sarem women's hospital in 2013. The patients' data including demographic information and risk factors for preterm labor were collected by a researcher-made-questionnaire. The data were analyzed by Mann-Whitney U, Fisher's exact and Chi square tests using SPSS 22 software. Findings The prevalence of preterm labor was found as 237 (9.75%). The maternal factors including body mass index (BMI), maternal age (more than 35 years), history of thyroid disease, intrauterine fetal death (IUFD), premature rupture of membranes (PROM), preeclampsia, parity, two or multiple pregnancies and abnormal volume of amniotic fluid showed significant correlation with the occurrence of spontaneous preterm labor (P <0.05). However, the other factors including hypertension history before pregnancy, history of abortion, history of diabetes and ectopic pregnancy did not show statistically significant correlation with preterm labor (P>0.05). Conclusion Maternal factors including BMI, maternal age, thyroid disease, IUFD, PROM, preeclampsia, parity, two or multiple pregnancies and abnormal volume of amniotic fluid can influence the occurrence of spontaneous preterm labor.

Iranian Journal of Pediatric Hematology & Oncology

Background: In various cancers, Ganoderic Acid A (GAA), an active triterpenoid derived from Ganod... more Background: In various cancers, Ganoderic Acid A (GAA), an active triterpenoid derived from Ganoderma Background: Thalassemia refers to a category of inherited disorders resulting from defects in synthesizing one or several chains of hemoglobin (Hb). The present study aimed to determine the frequency of alpha and beta-thalassemia mutations in Kurdistan province, Iran. Materials and Methods: In this retrospective cross-sectional study, the laboratory data of 340 patients with thalassemia (170 females and 170 males), who were candidates for genetic testing in Kurdistan province, were examined over ten years (2006-2016). The participants were Kurd couples selected from the premarital health screening program. Results: In this demographic study, 20 beta mutations and nine alpha mutations were identified. Among the beta-thalassemia mutations, intervening sequence or intron No. 2- first nucleotide change as splice site mutation (IVS-II-1) (26.1%), codons 8/9 (14.8%), and intervening seque...

Clinical Case Reports, 2021

Abstract The report of LMNB2‐related progressive myoclonus epilepsy and ataxia due to missense ho... more Abstract The report of LMNB2‐related progressive myoclonus epilepsy and ataxia due to missense homozygous c.473G>T variant.

Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic and potentially fatal complication of a... more Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic and potentially fatal complication of assisted reproductive technologies (ART) that can occur following controlled ovarian hyperstimulation (COH) after the administration of human chorionic gonadotropin (hCG) [1]. OHSS may develop early or late after exposure to hCG. Early OHSS occurs immediately after oocyte retrieval and reflects the effect of exogenous hCG, which is administered to achieve final follicular maturation following the excessive ovarian response to follicle-stimulating hormone (FSH). Late OHSS occurs 10 days or more after the ovulatory dose of hCG and is caused by endogenous hCG produced by the primary pregnancy; it may be more severe and more dangerous than the early form, and it is difficult to predict [2]. Whereas mild OHSS is of no clinical significance and does not require intervention, its severe form is difficult to control and treat, and is a life-threatening complication [3,4]. OHSS presents with the e...

New Cellular and Molecular Biotechnology Journal, 2015

Genetics in the Third Millennium, 2016

Breast cancer is the most important and prevalent cancer in women that can be managed if detected... more Breast cancer is the most important and prevalent cancer in women that can be managed if detected in the early stages. Recently, there has been much focus on the use of epigenetic changes such as DNA methylation in early detection of cancer. As recent research has put forth DOK7 CpG Island methylation as an epimarker, in the present study, using a quantification method, a cohort of Iranian sporadic breast cancer patients are studied compared to healthy women. In this matched case-control study (by ethnicity andage), blood samples were collected and the extracted DNA was controlled and after sonication, the MeDIP process started. The enrichment estimation is the MeDIP control step and after that, all of the H19-ICR, GAPDH-promoter and DOK7-CpG were standardized with appropriate efficiency for Real-Time PCR relative quantification process. Comparison of the Relative Quantification (RQ) of the enriched methylated DNA in the DOK7 gene CpG island in the two groups showed a significant di...

Caspian Journal of Health Research, 2021

Background: Despite the recommendations to follow guidelines for the prevention of COVID-19, diff... more Background: Despite the recommendations to follow guidelines for the prevention of COVID-19, different communities do not completely adhere to these guidelines. The aim of this study was to explore the barriers and facilitators of the adherence of the people of Sanandaj to the COVID-19 prevention guidelines. Materials & Methods: The present study was a qualitative content analysis study conducted in 2020. A purposive sampling method among the residents of Sanandaj, Iran was applied and continued until data saturation was achieved. Twenty semi-structured interviews were conducted with the participants. A verbatim transcription of interviews was analyzed through qualitative conventional content analysis. Results: Participants included 12 men and 8 women. Data analysis yielded 8 categories and 26 sub-categories regarding the barriers and facilitators of the participants’ adherence to the COVID-19 prevention guidelines. The barriers included myths, being under pressure, and letting and ...

Academic Medicine, 2015

Background and purpose: Partial knowledge is one of the main factors to be considered when dealin... more Background and purpose: Partial knowledge is one of the main factors to be considered when dealing with the improvement of the administration of Multiple Choice Questions (MCQ) in testing. Various strategies have been proposed for this factor in the traditional testing environment. Therefore, this study proposed a Confidence Based Assessment (CBA) as a pertinent solution and aims at comparing the effect of the CBA Scoring system with that of the conventional scoring systems (with and without negative score estimation as penalty) on the students’ scores and estimating their partial knowledge on clinical studies. Methods: This comparative study was conducted using a standardized clinical knowledge exam for 117 clinical students. After two-step training, both the conventional MCQ and CBA examination was given in a single session simultaneously. The exam included 100 questions and the volunteers were requested to complete a questionnaire regarding their attitude and satisfaction on thei...

Sarem Journal of Reproductive Medicine, 2019

Sarem Journal of Reproductive Medicine, 2017

Sarem Journal of Reproductive Medicine, 2017

In this investigation Murine Embryonic Stem (ES) cells were differentiated into endothelial cells... more In this investigation Murine Embryonic Stem (ES) cells were differentiated into endothelial cells. Materials and Methods: Murine ES cells (CCE cell line) exposed to Alpha-MEM medium containing 10% FBS for 4 days. Then obtained Flk-1 (Flk-1: Vascular Endothelial Growth Factor Receptor 2) Positive cells were cultuted in Endothelial Growth Medium-2 (EGM-2) until the last day of experiment. Differentiated cells were evaluated by immunocytochemistry, RT-PCR and Tube Formation Assays. Results: When the ES cells cultured in collagen coated dishes containing Alpha-MEM & FBS, Flk-1 positive cells were obtained. After transfering Flk-1 positive cells into fibronectin coated dishes containing EGM2, the cells were assumed a relatively uniform endothelial cell morphology and could be propagated and expanded. Immunocytochemical and RT-PCR analysis of differentiated cells showed that they take up acetylated low-density lipoprotein (LDL), express Flk-1, CD31 and bind the BS-l lectin. When placed in Matrigel, these Murine ES cell-derived endothelial cells formed capillary-like structures characteristic of endothelial cells Conclusion: ES cell-derived endothelial cells provide a novel means to examine the mechanisms of endothelial cell development, and may open up new therapeutic strategies.

Educational Research in Medical Sciences, 2013

Introduction: It is necessary to analyze the limitations and essentials of the medical students&#... more Introduction: It is necessary to analyze the limitations and essentials of the medical students' learning compatible with their future working environment in order to control the quality of the education. This study was designed to investigate the basics of learning and to prepare educational logbooks for all clinical departments at Kurdistan University of Medical Sciences in line with promotion of educational and students' assessment. Methods: This study was carried out in three phases. In the first phase, content was confirmed according to the expert views. In the second phase, materials were analyzed in terms of being core and non-core. In the third phase, prioritized subjects were summarized and locations selected for teaching each skill, rotation style, regulations and responsibilities of the students, assessment method and group's expectations from students were determined. The data were analyzed by analytic hierarchy process (AHP), comparison of the content of mat...

Sarem Journal of Reproductive Medicine, 2019

Endometriomas as a possible cause of reduced ovarian reserve in women with endometriosis [2] High... more Endometriomas as a possible cause of reduced ovarian reserve in women with endometriosis [2] High prevalence of endometriosis in infertile women with normal ovulation and normospermic partners [3] Laparoscopic colorectal resections with transvaginal specimen extraction for severe endometriosis [4] SEndometriosis and infertility: A committee opinion [5] Effect of endometriosis on IVF/ICSI outcome: Stage III/ IV endometriosis worsens cumulative pregnancy and live-born rates [6] Possible effects of endometriosis-related immune events on reproductive function [7] Endometriosis and infertility [8] Treatment of endometriosis as a priority before art [9] Efficacy analysis of an experience on development of the combination therapy protocol for endometriosis management [10] Endometriosis management; A survey on medical & laparoscopic treatment [11] The impact of endometrioma on IVF/ICSI outcomes: A systematic review and meta-analysis [12] Effects of ovarian endometrioma on embryo quality [13] Evidence linked treatment for endometriosis-associated infertility [14] Assessment of oocyte quality in polycystic ovarian syndrome and endometriosis by spindle imaging and reactive oxygen species levels in follicular fluid and its relationship with IVF-ET outcome Aims The women with infertility caused by endometriosis, need to use assisted reproductive technology after their treatment. The objective of this study was to evaluate fertility rate using ICSI in women with treated endometriosis compared to healthy women. Materials & Methods In this retrospective quasi-experimental study, endometriotic women (n=150) who were treated by endometriosis treatment protocol of Sarem Women's hospital were selected and their fertility rate was compared with that of normal women (n=150) who referred Sarem Women's hospital for ICSI due to obstructive azoospermia of their husbands. The data were analyzed by Spearman rank correlation coefficient, Mann-Whitney U, Fisher's exact and Chi-square tests using SPSS 22 software. Findings The mature oocytes number of case group was significantly lower than control group (p=0.036); however, the fertility rate did not show significant differences between groups (p=0.397). There was a reverse significant correlation between the grade of endometriosis with the total oocyte and mature oocyte counts in endometriotic patients after treatment (p=0.04 and p=0.016, respectively). Conclusion The fertility rate using ICSI method in women after endometriosis treatment is not different from healthy women.

Academic Medicine, 2009

Background and purpose: Problem-based learning (PBL) is a term used within education for a range ... more Background and purpose: Problem-based learning (PBL) is a term used within education for a range of teaching approaches that encourage students to learn through the structured exploration of a problem. Histology comes early in the curriculum and the medical students seem unable to see the value of the content, they don't appear to be motivated to learn the content. This project used PBL to help the students make the connection between the content and clinical aspects. Methods: Thirty six undergraduate medical students, 22 female and 14 male, enrolled in the histology course during the spring s emester of 2008. A survey which collected information relative to gender, course load, and workload and study time was used. The subjects were accessory glands of digestive system histology. The course is designed into four units: tree units of salivary glands, pancreas and gall bladder histology, were presented in a traditional lecture format; the fourth unit, liver was presented in a pro...

Sarem Journal of Reproductive Medicine, 2019

N. Evaluation the success rate of treatment failure in patients with unexplained repeated IVF tre... more N. Evaluation the success rate of treatment failure in patients with unexplained repeated IVF treatments immunization with lymphocytes (LIT) methods.

Sarem Journal of Reproductive Medicine, 2017

The histological features of ... [22] Splenosis: The autotransplantation of splenic tissue follow... more The histological features of ... [22] Splenosis: The autotransplantation of splenic tissue following injury to the spleen; report of two cases and review of the ... [23] A cause of massive gastrointestinal ... [24] Splenosis presenting as occult gastrointestinal ... [25] Splenosis: Ultrasound and CT findings in a case complicated by an intraperitoneal implant traumatic ... [26] Traumatic rupture of splenic tissue 13 years after splenectomy. A case ... [27] Pelvic splenosis mimicking endometriosis, causing low ... [28] Scintigraphic detection of splenosis causing ureteral compression and ... [29] Idiopathic thrombocytopenic purpura occurring in a subject previously splenectomized for traumatic splenic ... [30] Splenic sequestration of 99mTc labeled, heat treated red blood ... [31] A case report of abdominal splenosis-a practical mini-review for a ... [32] Splenosis; Review and report of subcutaneous splenic ... [33] Splenogonadal ... [34] Right splenorenal fusion with ... [35] Female splenic-gonadal fusion of the discontinuous ... [36] Thoracic splenosis: A diagnosis by history ... [37] Fine needle aspiration of thoracic splenosis ... [38] Parenchymal thoracic splenosis: History and nuclear imaging without invasive procedures may provide ... [39] Intrathoracic splenosis: Evaluation by 99mTc-labelled heat-denatured erythrocyte ... [40] MR features of abdominal ... [41] Clinical studies with spleen-specific radiolabeled ... [42] Scintigraphic detection of splenosis: Superiority of tomographic selective spleen ... [43] The born-again spleen. Return of splenic function after splenectomy for ... [44] Minimally invasive surgery in the treatment of ... Patient Information Spontaneous splenic transplantation and implant in in abdominal and pelvic regions is splenosis, which occurs after splenectomy followed by trauma and surgery. The clinical significance of splenic implant and the requirement for its removal are debatable. The cases of splenic implants in the pelvic zone have rarely been reported. Generally, these patients have no clinical features and are usually discovered by chance. In this report, a 36-year-old woman with the history of splenectomy after an accident at the age of 4 was introduced with Dysmenorrhea and infertility; the primary diagnosis was endometriosis, but pathology results reported the splenosis. Whole tissues and implants of spleen around the ovary and choledosac were removed; following this, the patient's pain was reduced and the overall health improved. Conclusion Splenosis can emerge as a benign condition in the abdomen and pelvis, and can show itself as a spread of tissue implants in the pelvic cavity. It can, however, easily be mistaken for endometriosis in imaging.

Sarem Journal of Reproductive Medicine, 2016

Predisposition to cancer and ... [8] Chromosomal radiosensitivity: A study of the chromosomal G(2... more Predisposition to cancer and ... [8] Chromosomal radiosensitivity: A study of the chromosomal G(2) assay in human blood lymphocytes indicating significant ... [9] The G2 ... [10] Is chromosome radiosensitivity and apoptotic response to irradiation correlated with cancer ... [11] Lymphocyte radiosensitivity in BRCA1 and BRCA2 mutation carriers and implications for breast cancer ... [12] Chromosomal radiosensitivity in breast cancer patients with a known or putative genetic ... [13] Increased chromosomal radiosensitivity in breast cancer patients: A comparison of two ... [14] Relationship between in vitro chromosomal radiosensitivity of peripheral ... [15] Heritability of cellular radiosensitivity ... [16] Nucleotide Excision Repair Syndromes ... [17] Xeroderma pigmentosum: A human disease in ... [18] Cytogenetic status of ataxia ... [19] Enhanced chromatid damage ... [20] Radiationinduced micronucleus induction in lymphocytes identifies a ... [21] Genotoxic effects of radiotherapy and ... [22] Chromosomes, cancer and ... [23] Chromosomal radiosensitivity in G2-phase lymphocytes identifies breast cancer ... [24] Increased G2 chromosomal radiosensitivity in cancer patients ... [25] Deficient DNA repair capacity, apredisposing ... [26] Genetic predisposition in ... [27] Evaluating the effects of genetic variants ... [28] G2 checkpoint control and G2 chromosomal radiosensitivity ... [29] DNA repair capacity as a possible biomarker ... [30] Chromosomal radiosensitivity as a ... [31] Validation of the cell cycle G(2) ... [32] Chromosomal radiosensitivity in ... [33] G2-phase chromosomal radiosensitivity ... [34] Spontaneous and radiation-induced ... [35] Chromosomal radiosensitivity in head and neck cancer patients: Evidence for ... Aims The use of non-invasive laboratory tests based on detection of biomarkers in the blood samples is a good strategy for early diagnosis of breast cancer. The lymphocyte radiosensitivity assessment can be a valuable method to diagnose breast cancer. The objective of this study was to investigate the radiosensitivity indices in sporadic breast cancer among Iranian women and to evaluate the potential of those indices for clinical use in early diagnosis of breast cancer and assessing its susceptibility. Materials & Methods The present study is a case-control that was conducted on 32 patients with sporadic breast cancer (patient group) and 30 healthy individuals (control group). The obtained blood samples of both groups were exposed to gamma-irradiation (0.4 Gy) and the level of chromosome breakage was determined based on the G2 chromosome breakage assay protocol. In the metaphase lymphocytes, the percentage of the abnormal cells was calculated as the radiosensitivity index for comparing the two groups. Data were analyzed by SPSS 19 software using student t-test, paired t-test, chi-square and Fisher exact test. Findings There was a significant difference in the percentage of the index of the abnormal cells after irradiation between two groups (p=0.001). The area under the curve (AUC) of the percentage of the abnormal cells and odds ratio (OR) were found as 0.725 with 3.818, respectively. The frequency of increased radiosensitivity based on this index (at 61% cutoff point), was 65.6% in the patients and about 33% in the control group. Conclusion The increased level of chromosome breakage following irradiation with gamma rays can be used as an early diagnostic biomarker of breast cancer or a possible indicator for breast cancer susceptibility.

Sarem Journal of Reproductive Medicine, 2017

Prospective randomized double-blind trial of 3 versus ... [19] Pre and post operative medical the... more Prospective randomized double-blind trial of 3 versus ... [19] Pre and post operative medical therapy ... [20] Revised American Society for Reproductive Medicine classification ... [21] Laparoscopy versus laparotomy in conservative ... [22] Laparoscopic surgery for pelvic pain associated with ... [23] Prospective, randomized, double-blind, controlled trial of laser laparoscopy in the ... [24] A prospective, randomized study comparing laparoscopic ... [25] Randomized clinical trial of two laparoscopic treatments of ... [26] Laparoscopic surgery for the management of ovarian ... [27] Hormone therapy for endometriosis and surgical ... [28] Laparoscopic excision of endometriosis: A randomized ... [29] The impact of treatment on the natural history ... [30] Subcutaneous depot medroxyprogesterone acetate versus leuprolide acetate in ... [31] Randomized trial of leuprolide versus continuous oral contraceptives in the treatment of endometriosis-associated ... [32] The impact of preoperative gonadotropin-releasing ... [33] Use of nafarelin versus placebo after reductive laparoscopic ... [34] ESHRE guideline: Management of women with ... [35] Long-term use of gonadotropin-releasing hormone analogs and hormone replacement therapy in the management of endometriosis ... Aims Laparoscopy is accepted as a golden standard method in treatment of endometriosis. The main objective of this study was to evaluate the effects of endometriosis management protocol using a combined drug-surgery treatment in various stages. Materials & Methods This non-experimental and interventional study was conducted on 47 patients with confirmed endometriosis in Sarem women's hospital (Tehran, Iran) in 2011-2012. The patients were divided into 4 groups according to the endometriosis stages of I to IV. Laparoscopy surgery (electro-coagulation and the adhesions removing) was performed for all patients, and then they treated for monthly injection of GnRH analogues. The second and third laparoscopy procedures were performed after 3 and 6 months for relevant groups, respectively. Findings In the stage-I endometriosis group 9 (90%) patients showed complete recovery after 3 months of treatment and 1 remained case showed complete recovery after 6 months. For stage-II and stage-III groups 16 (72.7%) and 5 (50%) cases showed complete recovery after 6 months of the treatment, respectively. The remained patients of stages II and III treated with GnRH for an additional 3 months and showed complete recovery (excluding one case) after 9 months. In Stage-IV, 2 (40%) cases showed complete recovery and 3 out of 5 patients were candidate to laparotomy. Conclusion The patients with high stages of endometriosis require more invasive and longer treatments. The success of combined drug-surgery treatment for patients in stage-I endometriosis is possible in the course of 3 months. It requires 6 months for patients in stage-II and between 6 to 9 months for patients in stage-III for complete recovery.

Behavior Modification, 2006

The Preferred Items and Experiences Questionnaire (PIEQ) is a new instrument to assess reinforcer... more The Preferred Items and Experiences Questionnaire (PIEQ) is a new instrument to assess reinforcer preferences in adolescents and adults. Research was conducted with college and high school students to develop the PIEQ, to examine its reliability with test-retest and internal consistency methods, and to test its validity. This research provides support for the PIEQ’s reliability and validity.

Sarem Journal of Reproductive Medicine, 2019

Estimating the causes of 4 million neonatal deaths in the year 2000 [2] The role of prenatal care... more Estimating the causes of 4 million neonatal deaths in the year 2000 [2] The role of prenatal care in recurrent preterm birth [3] The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity [4] Preterm delivery in women with pregestational diabetes mellitus or chronic hypertension relative to women with uncomplicated pregnancies [5] Investigating socioeconomic disparities in preterm birth: Evidence for selective study participation and selection bias [6] Socioeconomic disparities in preterm birth: causal pathways and mechanisms [7] Risk Factors for Preterm Birth and Small-for-gestational-age Births among Canadian Women [8] The preterm prediction study: a clinical risk assessment system [9] Paternal race is a risk factor for preterm birth [10] Risk factors for spontaneous preterm birth among aboriginal and non-aboriginal women in Manitoba [11] Prepregnancy body mass index is an independent risk factor for gestational hypertension, gestational diabetes, preterm labor, and small-and large-for-gestational-age infants [12] Urogenital Infections as a Risk Factor for Preterm Labor: A Hospital-Based Case-Control Study [13] Manual of high risk pregnancy and delivery [14] Prevalence of preterm delivery and its related factors in females referring to Shohada Tajrish Hospital [15] Effect of parity and advanced maternal age on obstetric outcome [16] Risk factors associated with preterm (< 37+ 0 weeks) and early preterm birth (< 32+ 0 weeks): Univariate and multivariate analysis of 106 345 singleton births from the 1994 statewide perinatal survey of Bavaria [17] Maternal factors associated to prematurity [18] Abortion and the risk of subsequent preterm birth: a systematic review with meta-analyses [19] Prevalence and maternal risk factors of preterm laboring in Qom, 2007 [20] Prevalence of preterm labor and it's complications in neonates of women with preeclampsia-eclampsia in Emam Reza Hospital [21] Survey of related factors in prematurity birth [22] Gestational diabetes mellitus and lesser degrees of pregnancy hyperglycemia: Association with increased risk of spontaneous preterm birth [23] The association between decreased amniotic fluid volume and treatment with nonsteroidal anti-inflammatory agents for preterm labor [24] Amniotic fluid abnormalities [25] Risks of preterm delivery and association with maternal age, birth order, and fetal gender Aims Preterm labor is the most common cause of mortality among neonates. The risk of spontaneous preterm labor is increased due to various maternal factors. Therefore, the objective of this study was to investigate the maternal factors involved in preterm labor. Materials & Methods This cross-sectional study as a descriptive-analytic and retrospective study was conducted on 2432 women referred to Sarem women's hospital in 2013. The patients' data including demographic information and risk factors for preterm labor were collected by a researcher-made-questionnaire. The data were analyzed by Mann-Whitney U, Fisher's exact and Chi square tests using SPSS 22 software. Findings The prevalence of preterm labor was found as 237 (9.75%). The maternal factors including body mass index (BMI), maternal age (more than 35 years), history of thyroid disease, intrauterine fetal death (IUFD), premature rupture of membranes (PROM), preeclampsia, parity, two or multiple pregnancies and abnormal volume of amniotic fluid showed significant correlation with the occurrence of spontaneous preterm labor (P <0.05). However, the other factors including hypertension history before pregnancy, history of abortion, history of diabetes and ectopic pregnancy did not show statistically significant correlation with preterm labor (P>0.05). Conclusion Maternal factors including BMI, maternal age, thyroid disease, IUFD, PROM, preeclampsia, parity, two or multiple pregnancies and abnormal volume of amniotic fluid can influence the occurrence of spontaneous preterm labor.

Iranian Journal of Pediatric Hematology & Oncology

Background: In various cancers, Ganoderic Acid A (GAA), an active triterpenoid derived from Ganod... more Background: In various cancers, Ganoderic Acid A (GAA), an active triterpenoid derived from Ganoderma Background: Thalassemia refers to a category of inherited disorders resulting from defects in synthesizing one or several chains of hemoglobin (Hb). The present study aimed to determine the frequency of alpha and beta-thalassemia mutations in Kurdistan province, Iran. Materials and Methods: In this retrospective cross-sectional study, the laboratory data of 340 patients with thalassemia (170 females and 170 males), who were candidates for genetic testing in Kurdistan province, were examined over ten years (2006-2016). The participants were Kurd couples selected from the premarital health screening program. Results: In this demographic study, 20 beta mutations and nine alpha mutations were identified. Among the beta-thalassemia mutations, intervening sequence or intron No. 2- first nucleotide change as splice site mutation (IVS-II-1) (26.1%), codons 8/9 (14.8%), and intervening seque...

Clinical Case Reports, 2021

Abstract The report of LMNB2‐related progressive myoclonus epilepsy and ataxia due to missense ho... more Abstract The report of LMNB2‐related progressive myoclonus epilepsy and ataxia due to missense homozygous c.473G>T variant.

Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic and potentially fatal complication of a... more Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic and potentially fatal complication of assisted reproductive technologies (ART) that can occur following controlled ovarian hyperstimulation (COH) after the administration of human chorionic gonadotropin (hCG) [1]. OHSS may develop early or late after exposure to hCG. Early OHSS occurs immediately after oocyte retrieval and reflects the effect of exogenous hCG, which is administered to achieve final follicular maturation following the excessive ovarian response to follicle-stimulating hormone (FSH). Late OHSS occurs 10 days or more after the ovulatory dose of hCG and is caused by endogenous hCG produced by the primary pregnancy; it may be more severe and more dangerous than the early form, and it is difficult to predict [2]. Whereas mild OHSS is of no clinical significance and does not require intervention, its severe form is difficult to control and treat, and is a life-threatening complication [3,4]. OHSS presents with the e...

New Cellular and Molecular Biotechnology Journal, 2015

Genetics in the Third Millennium, 2016

Breast cancer is the most important and prevalent cancer in women that can be managed if detected... more Breast cancer is the most important and prevalent cancer in women that can be managed if detected in the early stages. Recently, there has been much focus on the use of epigenetic changes such as DNA methylation in early detection of cancer. As recent research has put forth DOK7 CpG Island methylation as an epimarker, in the present study, using a quantification method, a cohort of Iranian sporadic breast cancer patients are studied compared to healthy women. In this matched case-control study (by ethnicity andage), blood samples were collected and the extracted DNA was controlled and after sonication, the MeDIP process started. The enrichment estimation is the MeDIP control step and after that, all of the H19-ICR, GAPDH-promoter and DOK7-CpG were standardized with appropriate efficiency for Real-Time PCR relative quantification process. Comparison of the Relative Quantification (RQ) of the enriched methylated DNA in the DOK7 gene CpG island in the two groups showed a significant di...

Caspian Journal of Health Research, 2021

Background: Despite the recommendations to follow guidelines for the prevention of COVID-19, diff... more Background: Despite the recommendations to follow guidelines for the prevention of COVID-19, different communities do not completely adhere to these guidelines. The aim of this study was to explore the barriers and facilitators of the adherence of the people of Sanandaj to the COVID-19 prevention guidelines. Materials & Methods: The present study was a qualitative content analysis study conducted in 2020. A purposive sampling method among the residents of Sanandaj, Iran was applied and continued until data saturation was achieved. Twenty semi-structured interviews were conducted with the participants. A verbatim transcription of interviews was analyzed through qualitative conventional content analysis. Results: Participants included 12 men and 8 women. Data analysis yielded 8 categories and 26 sub-categories regarding the barriers and facilitators of the participants’ adherence to the COVID-19 prevention guidelines. The barriers included myths, being under pressure, and letting and ...

Academic Medicine, 2015

Background and purpose: Partial knowledge is one of the main factors to be considered when dealin... more Background and purpose: Partial knowledge is one of the main factors to be considered when dealing with the improvement of the administration of Multiple Choice Questions (MCQ) in testing. Various strategies have been proposed for this factor in the traditional testing environment. Therefore, this study proposed a Confidence Based Assessment (CBA) as a pertinent solution and aims at comparing the effect of the CBA Scoring system with that of the conventional scoring systems (with and without negative score estimation as penalty) on the students’ scores and estimating their partial knowledge on clinical studies. Methods: This comparative study was conducted using a standardized clinical knowledge exam for 117 clinical students. After two-step training, both the conventional MCQ and CBA examination was given in a single session simultaneously. The exam included 100 questions and the volunteers were requested to complete a questionnaire regarding their attitude and satisfaction on thei...

Sarem Journal of Reproductive Medicine, 2019

Sarem Journal of Reproductive Medicine, 2017

Sarem Journal of Reproductive Medicine, 2017