Arianne Oriá - Academia.edu (original) (raw)
Papers by Arianne Oriá
PLOS ONE, 2019
An understanding of species' morphological and physiological parameters is crucial to developing ... more An understanding of species' morphological and physiological parameters is crucial to developing conservation strategies for wild animals kept in human care. Detailed information is lacking for crab-eating fox (Cerdocyon thous) eyes and adnexa. Therefore, the aim of this study was to describe anatomical, histological and computed tomography (CT) features of the eye and adnexa in crab-eating fox, compared to domestic dogs. CT of the eye and adnexa of one live animal and a frozen specimen was performed for anatomical identification. In addition, the heads of five animals of each species were fixed in 10% buffered formalin for gross anatomical description of the eye and adnexa using topographic dissection and exenteration techniques. All steps were photographed and features such as location, shape, and distances and relationships between structures were described. For histological evaluation, two eyes of each species were fixed in 10% buffered formalin, processed by routine paraffin inclusion technique and stained with hematoxylin and eosin. The CT scan was difficult to evaluate, mainly that of the frozen head, which did not provide good definition of the soft tissues; nevertheless, it demonstrated the potential for structure visualization and description. The gross anatomical and histological evaluations showed the presence of eyelashes on the upper eyelid and of upper and lower lacrimal points, an incomplete orbit with supraorbital ligament, slightly exposed sclera with discretely pigmented limbus and pigmentation throughout the conjunctiva, and a slit-shaped pupil. Hematoxylin and eosin staining demonstrated structural similarities between the crab-eating fox and domestic dog. Thus, the possibility of using the domestic dog as a study model for the preventive and therapeutic management of wild dogs kept in human care is demonstrated.
The aim of this study was to evaluate four protocols of loco regional anesthesia for ophthalmic p... more The aim of this study was to evaluate four protocols of loco regional anesthesia for ophthalmic procedures that could provide safety and life support, in addition to maintain intraocular pressure stable, with eye centralization and eyelid akinesia. 20 New Zealand rabbits were used to perform local anesthesia by retrobulbar block with four protocols: 2% lidocaine with epinephrine, 2% lidocaine without epinephrine associated with tramadol, 1% ropivacaine and bupivacaine 0.5 %. Each animal received an anesthetic volume of 1 mL. All anesthetic protocols used promoted eyelid akinesia and centralization of the eye during the assessment period. The retrobulbar block with the proposed anesthetic protocols proved to be feasible and safe for the maintenance of intraocular pressure, invasive blood pressure and pupillary diameter and can be used in intraocular surgeries, respecting the time of action of each anesthetic. All protocols showed an excellent blockage action but bupivacaine promoted ...
Acta Veterinaria Scandinavica
Background: The tear film is a trilaminar fluid composed mainly of lipids, electrolytes, proteins... more Background: The tear film is a trilaminar fluid composed mainly of lipids, electrolytes, proteins and water. It is responsible for lubrication, nutrition and protection against microbial and toxic agents. Disruption of any these components may weaken the ocular surface, making it more susceptible to disease. Increasing evidence suggests that qualitative tear film deficiencies are an important predisposing factor or cause of some of the most common and challenging ocular diseases in cats, including conjunctivitis, corneal ulcer, spontaneous chronic corneal epithelial defects (SCCED), pigmentary keratitis, corneal sequestrum and dry eye syndrome. The aim of this study was to describe the tear ferning test in healthy cats and to compare the results by using two grading scales for humans. Tear samples were collected using Schirmer tear test (STT) strips from 60 healthy cats, and, after centrifuging the strips to obtain the samples, the aliquot was placed on clean microscope glass until it dried and the tear ferning patterns were observed under a polarized light microscope and classified according to the Rolando and Masmali grading scales. Results: Ferning patterns in the lower grades showed full crystallization with high density, without gaps between the ferns and branches, forming several nuclei that were easily distinguished. According to the Rolando scale, 50% (60/120), 46.6% (56/120) and 3.4% (4/120) of eyes showed type I, II and III patterns, respectively. According to the Masmali scale, 15% (18/120), 56.6% (68/120 eyes) and 28.4% (34/120) of eyes showed grade 0, 1 and 2 patterns, respectively. No difference was observed between the right and left eyes for both Rolando (P = 0.225) and Masmali (P = 0.683) scales. Conclusions: The tear ferning test is a qualitative test that can be used in cats as a complementary evaluation of the ocular surface. While the Rolando scale showed an increased prevalence of types I and II, the Masmali scale showed an increased prevalence of grades 1 and 2. This can be attributed to the species-specific differences between human and feline tear film. So Masmali grade 2 can be considered a normal tear pattern for the species, because all the cats used in study were clinically healthy. For this reason, future complementary studies are necessary for comparing healthy eyes and eyes with different ocular surface disease in cats. Both scales can be feasible options for grading tear crystallization in cats, but as Rolando scale included 96.6% of the samples in the 2 types that are considered normal for humans, we think that this scale seemed to be more precise to classify crystallization pattern in cats. The crystallization patterns observed in this study can form the basis for standardizing ocular surface parameters in cats.
Frontiers in Veterinary Science
Ciência Rural
ABSTRACT: This study aimed to describe strip meniscometry test values for Amazona aestiva (turquo... more ABSTRACT: This study aimed to describe strip meniscometry test values for Amazona aestiva (turquoise-fronted amazon), Ara ararauna (blue-and-yellow macaw) and Caiman latirostris (broad-snouted caiman). Twenty-one Amazona aestiva and 11 Ara ararauna, healthy, adults of unknown sex, and 37 healthy adult Caiman latirostris (27 males and 10 females), were used in this investigation. All animals were manually restrained and I-Tear® Test strip was inserted for 5 s at the edge of the lower tear meniscus, without touching the eyelid or ocular surface, immediately followed by measurement (millimeter per 5 seconds). No differences were reported between left and right eyes for Amazona aestiva (P = 0.824), Ara ararauna (P = 0.262) and Caiman latirostris (P = 0.679). Median and confidence interval were 2.0 (2.25-3.56) mm/5s for Amazona aestiva, 3.0 (2.93-4.15) mm/5s for Ara ararauna and 3.0 (2.55-4.90) mm/5 s for Caiman latirostris. These values for strip meniscometry tear test can be used as pa...
Pesquisa Veterinária Brasileira
ABSTRACT: The aim of this study was to describe the modified Schirmer tear test (mSTT), intraocul... more ABSTRACT: The aim of this study was to describe the modified Schirmer tear test (mSTT), intraocular pressure (IOP) by rebound tonometry and palpebral fissure length (PFL) in blue-fronted Amazon parrots (Amazona aestiva). Thirty-five healthy adult animals from a conservation breeding center in Brazil were used in this study. Modified Schirmer tear test, rebound tonometry and PFL measurements were performed in both eyes, with birds under physical restraint. Mean mSTT was 6.2±0.1mm/min and mean IOP was 6.4±0.1mmHg, while PFL was 10.1±0.1mm. A moderate correlation was seen between mSTT and PFL for OD (ρ=0.14) and OS (ρ=0.20). The results provide ophthalmic tests reference values for A. aestiva.
Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Este artigo aborda as principais afecções da córnea em pequenos animais, bem como sua terapia esp... more Este artigo aborda as principais afecções da córnea em pequenos animais, bem como sua terapia específica.
BMC Veterinary Research
The original article [1] contained an error whereby the respective legends of Figs. 2 and 3 were ... more The original article [1] contained an error whereby the respective legends of Figs. 2 and 3 were mistakenly interchanged. This error has now been amended.
Avian Biology Research
The aim of the present study was to determine reference values for selected ophthalmic diagnostic... more The aim of the present study was to determine reference values for selected ophthalmic diagnostic tests in the Caatinga Parakeet ( Eupsittula cactorum) and Yellow-chevroned Parakeet ( Brotogeris chiriri). Healthy adults of undetermined gender (15 Caatinga Parakeets and 13 Yellow-chevroned Parakeets), kept at the Triage Center of Wild Animals (CETAS, Salvador, Bahia, Brazil), were used in this investigation. Ophthalmic diagnostic tests were conducted in both eyes, including culture of the conjunctival bacterial flora with antimicrobial susceptibility test, conjunctival cytology, evaluation of tear production by phenol red thread test (PRTT) and endodontic absorbent paper point tear test (EAPPTT), measurements of palpebral fissure length (PFL) and corneal touch threshold (CTT). There was bacterial growth in 97.5% of the evaluated samples, with predominance of Grampositive microorganisms. The antimicrobial susceptibility test showed ciprofloxacin to be the most effective antibiotic. Th...
Journal of medical primatology, Jan 24, 2018
To evaluate the composition and characteristics of capuchin monkey (CM) tear film. Eleven CM (Sap... more To evaluate the composition and characteristics of capuchin monkey (CM) tear film. Eleven CM (Sapajus sp.) was evaluated. Strip meniscometry test (SMT), osmolarity, and tear ferning test (TFT) (by Rolando and Masmali scales) were assessed. Tear protein profile (SDS-PAGE), and total protein, albumin, urea, glucose, and cholesterol levels in tear film and blood serum were screened. Median ± semi-interquatil range for SMT and osmolarity values were 8.0 ± 1.625 and 303.0 ± 9.875, respectively. TFT for Rolando was 2.0 ± 0.5, and Masmali was 2.0 ± 0.0. Monkeys's tear obtained type II and III for Rolando, and 1 and 2 for Masmali. Tear components showed protein bands among 23-217 kDa, and presence of albumin, urea, glucose, and cholesterol. The results of SMT, osmolarity, TFT, SDS-PAGE, and tear biochemistry may serve as a reference baseline for CM, and the data may serve as a basis for future experimental model evaluations.
PloS one, 2017
The objective of this study was to describe the anatomic and histologic features of the Sapajus s... more The objective of this study was to describe the anatomic and histologic features of the Sapajus sp. eye, comparing similarities and differences of humans and other species of non-human primates for biomedical research purposes. Computed tomography (CT) of adnexa, eye and orbit live animal, as well as formolized pieces of the same structures of Sapajus sp. for anatomical and histological study were also performed. The anatomical description of the eye and adnexa was performed using the techniques of topographic dissection and exenteration. Histological fragments were fixated in buffered formalin 10%, processed by the routine paraffin inclusion technique, stained with hematoxylin-eosin and special stains. CT scan evaluation showed no differences between the live animal and the formolized head on identification of visual apparatus structures. Anatomic and histologic evaluation revealed rounded orbit, absence of the supraorbital foramen and frontal notch, little exposure of the sclera, ...
Veterinary ophthalmology, Jan 7, 2017
To evaluate and compare three tear sampling methods using two grading scales for administering th... more To evaluate and compare three tear sampling methods using two grading scales for administering the tear ferning test (TFT) to healthy dogs. In total, 90 dogs (180 eyes) were subjected to tear sampling using millimetered strips, reused after the Schirmer tear test (STT) (Schirmer group, SG). Then, the dogs were subdivided into three groups according to sampling approach: micropipette (MPG), microcapillary (MCG), and Schirmer sample 2 (S2G). The collected tears were dried on a clean microscope glass slide at room temperature and humidity. The ferning patterns were observed under a polarized light microscope and classified according to the Rolando and Masmali grading scales. Although all three methods were feasible, the STT was easier to perform in clinical settings. Type I and Grade 1 were the most commonly observed (64.17% and 61.7%, respectively) regardless of collection method. There was no significant difference between the STT median values and the TFT classifications. The TFT is...
Ciência Rural
ABSTRACT: This study aimed to determine and compare corneal sensitivity values in different regio... more ABSTRACT: This study aimed to determine and compare corneal sensitivity values in different regions of the cornea in five horse breeds. One hundred and forty five healthy horses, adults of both sexes, of the following breeds - Arabian horse (AH; n=20), Mangalarga Marchador (MM; n=50), Pure Blood Lusitano (PBL; n=35), Quarter Horse (QH; n=20), Brazilian Sport Horse (BSH; n=20)-were investigated. Corneal touch threshold (CTT) was measured with a Cochet-Bonnet esthesiometer in five different corneal regions. Measurements of the median central CTT were: 4.50±0.50cm (AH), 3.50±0.56cm (MM), 3.00±0.25cm (PBL), 2.50±0.44cm (QH) and 2.50±0.00cm (BSH). The central region was the most sensitive and the dorsal region the least sensitive corneal region for all breeds. CTT values differed for corneal regions and horse breeds. The CTT values were different among the corneal regions and the horse breeds. Arabian horses presented higher sensitivity values being the most sensitive in all of the corne...
Pubvet
PUBVET, Publicações em Medicina Veterinária e Zootecnia. Viabilidade microbiológica do soro sangü... more PUBVET, Publicações em Medicina Veterinária e Zootecnia. Viabilidade microbiológica do soro sangüíneo autólogo como coadjuvante no tratamento de úlceras de córnea em cães (Canis familiaris, LINNAEUS, 1758)
Journal of Avian Medicine and Surgery
Safe and effective sedation protocols are important for chemical restraint of birds in clinical a... more Safe and effective sedation protocols are important for chemical restraint of birds in clinical and diagnostic procedures, such as clinical evaluations, radiographic positioning, and blood collection. These protocols may reduce stress and ease the management of wild-caught birds, which are susceptible to injury or death when exposed to stressful situations. We compare the sedative effect of intranasal midazolam in wild-caught blue-fronted (Amazona aestiva) and orange-winged (Amazona amazonica) Amazon parrots. Ten adult parrots of each species (n = 20), of unknown sex, weighing 0.337 ± 0.04 (blue-fronted) and 0.390 ± 0.03 kg (orange-winged), kg were used. Midazolam (2 mg/kg) was administered intranasally and the total volume of the drug was divided equally between the 2 nostrils. Onset time and total sedation time were assessed. Satisfactory sedation for clinical evaluation was induced in all birds. Onset time and total sedation times were similar in both species: 5.36 ± 1.16 and 25.40 ± 5.72 minutes, respectively, for blue-fronted Amazons and 5.09 ± 0.89 and 27.10 ± 3.73 minutes, respectively, for orange-winged Amazons. A total of 15 animals showed absence of vocalization, with moderate muscle relaxation and wing movement upon handling, and 2 animals presented with lateral recumbence, with intense muscle relaxation and no wing movement, requiring no restraint. Three blue-fronted Amazons had no effective sedation. Intranasally administered midazolam at a dose of 2 mg/kg effectively promoted sedative effects with a short latency time and fast recovery in wild-caught parrots.
Journal of Medical Primatology
Epidural anesthesia is indicated to decrease other anesthetic requirements, prolong analgesia, an... more Epidural anesthesia is indicated to decrease other anesthetic requirements, prolong analgesia, and reduce side effects. In primates, its use has been scarcely described. The aim was to evaluate the cardiorespiratory effects of epidural anesthesia with lidocaine and dexmedetomidine (DEX) or morphine (MOR). Ten female capuchin monkeys (Sapajus sp.) received epidural anesthesia with lidocaine and DEX or MOR under general anesthesia. There was a significant decrease in heart rate with DEX and in blood pressure in all groups, with no changes in SpO . There was a significant reduction in rectal temperature over time. A stable cardiac rhythm was observed; however, there was a prolonged QT interval with DEX. Epidural anesthesia with access to the lumbosacral space was safe and easy to perform. DEX decreased heart rate. All groups promoted a decrease in rectal temperature with respiratory and cardiac rhythm stability; however, hypotension should be considered.
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians, 2017
The collared anteater ( Tamandua tetradactyla ) is adapted to a variety of habitats. It is a soli... more The collared anteater ( Tamandua tetradactyla ) is adapted to a variety of habitats. It is a solitary species for which no reference values for ophthalmic tests have been established. Eight animals ranging from 1 to 4 yr of age, two males and six females, were manually restrained for assessment. Ophthalmic tests included evaluation of tear production by Schirmer tear test 1 (STT1), endodontic absorbent paper point tear test (EAPPTT), palpebral fissure length (PFL), culture of the conjunctival bacterial flora, and antimicrobial susceptibility test. Median ± semi-interquartile range (S-IQR) STT1, EAPPTT, and PFL were 8.50 ± 4.13 mm/min, 14.13 ± 3.24 mm/min, and 15.91 ± 2.51 mm, respectively. Bacterial growth was present in 100% of the samples, with predominance of Gram-positive bacteria (70.27%). Staphylococcus spp. was the most frequently isolated genus. Antimicrobial susceptibility test showed sensitivity of Staphylococcus epidermidis and Staphylococcus spp. to neomycin, tobramycin,...
PLOS ONE, 2019
An understanding of species' morphological and physiological parameters is crucial to developing ... more An understanding of species' morphological and physiological parameters is crucial to developing conservation strategies for wild animals kept in human care. Detailed information is lacking for crab-eating fox (Cerdocyon thous) eyes and adnexa. Therefore, the aim of this study was to describe anatomical, histological and computed tomography (CT) features of the eye and adnexa in crab-eating fox, compared to domestic dogs. CT of the eye and adnexa of one live animal and a frozen specimen was performed for anatomical identification. In addition, the heads of five animals of each species were fixed in 10% buffered formalin for gross anatomical description of the eye and adnexa using topographic dissection and exenteration techniques. All steps were photographed and features such as location, shape, and distances and relationships between structures were described. For histological evaluation, two eyes of each species were fixed in 10% buffered formalin, processed by routine paraffin inclusion technique and stained with hematoxylin and eosin. The CT scan was difficult to evaluate, mainly that of the frozen head, which did not provide good definition of the soft tissues; nevertheless, it demonstrated the potential for structure visualization and description. The gross anatomical and histological evaluations showed the presence of eyelashes on the upper eyelid and of upper and lower lacrimal points, an incomplete orbit with supraorbital ligament, slightly exposed sclera with discretely pigmented limbus and pigmentation throughout the conjunctiva, and a slit-shaped pupil. Hematoxylin and eosin staining demonstrated structural similarities between the crab-eating fox and domestic dog. Thus, the possibility of using the domestic dog as a study model for the preventive and therapeutic management of wild dogs kept in human care is demonstrated.
The aim of this study was to evaluate four protocols of loco regional anesthesia for ophthalmic p... more The aim of this study was to evaluate four protocols of loco regional anesthesia for ophthalmic procedures that could provide safety and life support, in addition to maintain intraocular pressure stable, with eye centralization and eyelid akinesia. 20 New Zealand rabbits were used to perform local anesthesia by retrobulbar block with four protocols: 2% lidocaine with epinephrine, 2% lidocaine without epinephrine associated with tramadol, 1% ropivacaine and bupivacaine 0.5 %. Each animal received an anesthetic volume of 1 mL. All anesthetic protocols used promoted eyelid akinesia and centralization of the eye during the assessment period. The retrobulbar block with the proposed anesthetic protocols proved to be feasible and safe for the maintenance of intraocular pressure, invasive blood pressure and pupillary diameter and can be used in intraocular surgeries, respecting the time of action of each anesthetic. All protocols showed an excellent blockage action but bupivacaine promoted ...
Acta Veterinaria Scandinavica
Background: The tear film is a trilaminar fluid composed mainly of lipids, electrolytes, proteins... more Background: The tear film is a trilaminar fluid composed mainly of lipids, electrolytes, proteins and water. It is responsible for lubrication, nutrition and protection against microbial and toxic agents. Disruption of any these components may weaken the ocular surface, making it more susceptible to disease. Increasing evidence suggests that qualitative tear film deficiencies are an important predisposing factor or cause of some of the most common and challenging ocular diseases in cats, including conjunctivitis, corneal ulcer, spontaneous chronic corneal epithelial defects (SCCED), pigmentary keratitis, corneal sequestrum and dry eye syndrome. The aim of this study was to describe the tear ferning test in healthy cats and to compare the results by using two grading scales for humans. Tear samples were collected using Schirmer tear test (STT) strips from 60 healthy cats, and, after centrifuging the strips to obtain the samples, the aliquot was placed on clean microscope glass until it dried and the tear ferning patterns were observed under a polarized light microscope and classified according to the Rolando and Masmali grading scales. Results: Ferning patterns in the lower grades showed full crystallization with high density, without gaps between the ferns and branches, forming several nuclei that were easily distinguished. According to the Rolando scale, 50% (60/120), 46.6% (56/120) and 3.4% (4/120) of eyes showed type I, II and III patterns, respectively. According to the Masmali scale, 15% (18/120), 56.6% (68/120 eyes) and 28.4% (34/120) of eyes showed grade 0, 1 and 2 patterns, respectively. No difference was observed between the right and left eyes for both Rolando (P = 0.225) and Masmali (P = 0.683) scales. Conclusions: The tear ferning test is a qualitative test that can be used in cats as a complementary evaluation of the ocular surface. While the Rolando scale showed an increased prevalence of types I and II, the Masmali scale showed an increased prevalence of grades 1 and 2. This can be attributed to the species-specific differences between human and feline tear film. So Masmali grade 2 can be considered a normal tear pattern for the species, because all the cats used in study were clinically healthy. For this reason, future complementary studies are necessary for comparing healthy eyes and eyes with different ocular surface disease in cats. Both scales can be feasible options for grading tear crystallization in cats, but as Rolando scale included 96.6% of the samples in the 2 types that are considered normal for humans, we think that this scale seemed to be more precise to classify crystallization pattern in cats. The crystallization patterns observed in this study can form the basis for standardizing ocular surface parameters in cats.
Frontiers in Veterinary Science
Ciência Rural
ABSTRACT: This study aimed to describe strip meniscometry test values for Amazona aestiva (turquo... more ABSTRACT: This study aimed to describe strip meniscometry test values for Amazona aestiva (turquoise-fronted amazon), Ara ararauna (blue-and-yellow macaw) and Caiman latirostris (broad-snouted caiman). Twenty-one Amazona aestiva and 11 Ara ararauna, healthy, adults of unknown sex, and 37 healthy adult Caiman latirostris (27 males and 10 females), were used in this investigation. All animals were manually restrained and I-Tear® Test strip was inserted for 5 s at the edge of the lower tear meniscus, without touching the eyelid or ocular surface, immediately followed by measurement (millimeter per 5 seconds). No differences were reported between left and right eyes for Amazona aestiva (P = 0.824), Ara ararauna (P = 0.262) and Caiman latirostris (P = 0.679). Median and confidence interval were 2.0 (2.25-3.56) mm/5s for Amazona aestiva, 3.0 (2.93-4.15) mm/5s for Ara ararauna and 3.0 (2.55-4.90) mm/5 s for Caiman latirostris. These values for strip meniscometry tear test can be used as pa...
Pesquisa Veterinária Brasileira
ABSTRACT: The aim of this study was to describe the modified Schirmer tear test (mSTT), intraocul... more ABSTRACT: The aim of this study was to describe the modified Schirmer tear test (mSTT), intraocular pressure (IOP) by rebound tonometry and palpebral fissure length (PFL) in blue-fronted Amazon parrots (Amazona aestiva). Thirty-five healthy adult animals from a conservation breeding center in Brazil were used in this study. Modified Schirmer tear test, rebound tonometry and PFL measurements were performed in both eyes, with birds under physical restraint. Mean mSTT was 6.2±0.1mm/min and mean IOP was 6.4±0.1mmHg, while PFL was 10.1±0.1mm. A moderate correlation was seen between mSTT and PFL for OD (ρ=0.14) and OS (ρ=0.20). The results provide ophthalmic tests reference values for A. aestiva.
Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Este artigo aborda as principais afecções da córnea em pequenos animais, bem como sua terapia esp... more Este artigo aborda as principais afecções da córnea em pequenos animais, bem como sua terapia específica.
BMC Veterinary Research
The original article [1] contained an error whereby the respective legends of Figs. 2 and 3 were ... more The original article [1] contained an error whereby the respective legends of Figs. 2 and 3 were mistakenly interchanged. This error has now been amended.
Avian Biology Research
The aim of the present study was to determine reference values for selected ophthalmic diagnostic... more The aim of the present study was to determine reference values for selected ophthalmic diagnostic tests in the Caatinga Parakeet ( Eupsittula cactorum) and Yellow-chevroned Parakeet ( Brotogeris chiriri). Healthy adults of undetermined gender (15 Caatinga Parakeets and 13 Yellow-chevroned Parakeets), kept at the Triage Center of Wild Animals (CETAS, Salvador, Bahia, Brazil), were used in this investigation. Ophthalmic diagnostic tests were conducted in both eyes, including culture of the conjunctival bacterial flora with antimicrobial susceptibility test, conjunctival cytology, evaluation of tear production by phenol red thread test (PRTT) and endodontic absorbent paper point tear test (EAPPTT), measurements of palpebral fissure length (PFL) and corneal touch threshold (CTT). There was bacterial growth in 97.5% of the evaluated samples, with predominance of Grampositive microorganisms. The antimicrobial susceptibility test showed ciprofloxacin to be the most effective antibiotic. Th...
Journal of medical primatology, Jan 24, 2018
To evaluate the composition and characteristics of capuchin monkey (CM) tear film. Eleven CM (Sap... more To evaluate the composition and characteristics of capuchin monkey (CM) tear film. Eleven CM (Sapajus sp.) was evaluated. Strip meniscometry test (SMT), osmolarity, and tear ferning test (TFT) (by Rolando and Masmali scales) were assessed. Tear protein profile (SDS-PAGE), and total protein, albumin, urea, glucose, and cholesterol levels in tear film and blood serum were screened. Median ± semi-interquatil range for SMT and osmolarity values were 8.0 ± 1.625 and 303.0 ± 9.875, respectively. TFT for Rolando was 2.0 ± 0.5, and Masmali was 2.0 ± 0.0. Monkeys's tear obtained type II and III for Rolando, and 1 and 2 for Masmali. Tear components showed protein bands among 23-217 kDa, and presence of albumin, urea, glucose, and cholesterol. The results of SMT, osmolarity, TFT, SDS-PAGE, and tear biochemistry may serve as a reference baseline for CM, and the data may serve as a basis for future experimental model evaluations.
PloS one, 2017
The objective of this study was to describe the anatomic and histologic features of the Sapajus s... more The objective of this study was to describe the anatomic and histologic features of the Sapajus sp. eye, comparing similarities and differences of humans and other species of non-human primates for biomedical research purposes. Computed tomography (CT) of adnexa, eye and orbit live animal, as well as formolized pieces of the same structures of Sapajus sp. for anatomical and histological study were also performed. The anatomical description of the eye and adnexa was performed using the techniques of topographic dissection and exenteration. Histological fragments were fixated in buffered formalin 10%, processed by the routine paraffin inclusion technique, stained with hematoxylin-eosin and special stains. CT scan evaluation showed no differences between the live animal and the formolized head on identification of visual apparatus structures. Anatomic and histologic evaluation revealed rounded orbit, absence of the supraorbital foramen and frontal notch, little exposure of the sclera, ...
Veterinary ophthalmology, Jan 7, 2017
To evaluate and compare three tear sampling methods using two grading scales for administering th... more To evaluate and compare three tear sampling methods using two grading scales for administering the tear ferning test (TFT) to healthy dogs. In total, 90 dogs (180 eyes) were subjected to tear sampling using millimetered strips, reused after the Schirmer tear test (STT) (Schirmer group, SG). Then, the dogs were subdivided into three groups according to sampling approach: micropipette (MPG), microcapillary (MCG), and Schirmer sample 2 (S2G). The collected tears were dried on a clean microscope glass slide at room temperature and humidity. The ferning patterns were observed under a polarized light microscope and classified according to the Rolando and Masmali grading scales. Although all three methods were feasible, the STT was easier to perform in clinical settings. Type I and Grade 1 were the most commonly observed (64.17% and 61.7%, respectively) regardless of collection method. There was no significant difference between the STT median values and the TFT classifications. The TFT is...
Ciência Rural
ABSTRACT: This study aimed to determine and compare corneal sensitivity values in different regio... more ABSTRACT: This study aimed to determine and compare corneal sensitivity values in different regions of the cornea in five horse breeds. One hundred and forty five healthy horses, adults of both sexes, of the following breeds - Arabian horse (AH; n=20), Mangalarga Marchador (MM; n=50), Pure Blood Lusitano (PBL; n=35), Quarter Horse (QH; n=20), Brazilian Sport Horse (BSH; n=20)-were investigated. Corneal touch threshold (CTT) was measured with a Cochet-Bonnet esthesiometer in five different corneal regions. Measurements of the median central CTT were: 4.50±0.50cm (AH), 3.50±0.56cm (MM), 3.00±0.25cm (PBL), 2.50±0.44cm (QH) and 2.50±0.00cm (BSH). The central region was the most sensitive and the dorsal region the least sensitive corneal region for all breeds. CTT values differed for corneal regions and horse breeds. The CTT values were different among the corneal regions and the horse breeds. Arabian horses presented higher sensitivity values being the most sensitive in all of the corne...
Pubvet
PUBVET, Publicações em Medicina Veterinária e Zootecnia. Viabilidade microbiológica do soro sangü... more PUBVET, Publicações em Medicina Veterinária e Zootecnia. Viabilidade microbiológica do soro sangüíneo autólogo como coadjuvante no tratamento de úlceras de córnea em cães (Canis familiaris, LINNAEUS, 1758)
Journal of Avian Medicine and Surgery
Safe and effective sedation protocols are important for chemical restraint of birds in clinical a... more Safe and effective sedation protocols are important for chemical restraint of birds in clinical and diagnostic procedures, such as clinical evaluations, radiographic positioning, and blood collection. These protocols may reduce stress and ease the management of wild-caught birds, which are susceptible to injury or death when exposed to stressful situations. We compare the sedative effect of intranasal midazolam in wild-caught blue-fronted (Amazona aestiva) and orange-winged (Amazona amazonica) Amazon parrots. Ten adult parrots of each species (n = 20), of unknown sex, weighing 0.337 ± 0.04 (blue-fronted) and 0.390 ± 0.03 kg (orange-winged), kg were used. Midazolam (2 mg/kg) was administered intranasally and the total volume of the drug was divided equally between the 2 nostrils. Onset time and total sedation time were assessed. Satisfactory sedation for clinical evaluation was induced in all birds. Onset time and total sedation times were similar in both species: 5.36 ± 1.16 and 25.40 ± 5.72 minutes, respectively, for blue-fronted Amazons and 5.09 ± 0.89 and 27.10 ± 3.73 minutes, respectively, for orange-winged Amazons. A total of 15 animals showed absence of vocalization, with moderate muscle relaxation and wing movement upon handling, and 2 animals presented with lateral recumbence, with intense muscle relaxation and no wing movement, requiring no restraint. Three blue-fronted Amazons had no effective sedation. Intranasally administered midazolam at a dose of 2 mg/kg effectively promoted sedative effects with a short latency time and fast recovery in wild-caught parrots.
Journal of Medical Primatology
Epidural anesthesia is indicated to decrease other anesthetic requirements, prolong analgesia, an... more Epidural anesthesia is indicated to decrease other anesthetic requirements, prolong analgesia, and reduce side effects. In primates, its use has been scarcely described. The aim was to evaluate the cardiorespiratory effects of epidural anesthesia with lidocaine and dexmedetomidine (DEX) or morphine (MOR). Ten female capuchin monkeys (Sapajus sp.) received epidural anesthesia with lidocaine and DEX or MOR under general anesthesia. There was a significant decrease in heart rate with DEX and in blood pressure in all groups, with no changes in SpO . There was a significant reduction in rectal temperature over time. A stable cardiac rhythm was observed; however, there was a prolonged QT interval with DEX. Epidural anesthesia with access to the lumbosacral space was safe and easy to perform. DEX decreased heart rate. All groups promoted a decrease in rectal temperature with respiratory and cardiac rhythm stability; however, hypotension should be considered.
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians, 2017
The collared anteater ( Tamandua tetradactyla ) is adapted to a variety of habitats. It is a soli... more The collared anteater ( Tamandua tetradactyla ) is adapted to a variety of habitats. It is a solitary species for which no reference values for ophthalmic tests have been established. Eight animals ranging from 1 to 4 yr of age, two males and six females, were manually restrained for assessment. Ophthalmic tests included evaluation of tear production by Schirmer tear test 1 (STT1), endodontic absorbent paper point tear test (EAPPTT), palpebral fissure length (PFL), culture of the conjunctival bacterial flora, and antimicrobial susceptibility test. Median ± semi-interquartile range (S-IQR) STT1, EAPPTT, and PFL were 8.50 ± 4.13 mm/min, 14.13 ± 3.24 mm/min, and 15.91 ± 2.51 mm, respectively. Bacterial growth was present in 100% of the samples, with predominance of Gram-positive bacteria (70.27%). Staphylococcus spp. was the most frequently isolated genus. Antimicrobial susceptibility test showed sensitivity of Staphylococcus epidermidis and Staphylococcus spp. to neomycin, tobramycin,...