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Papers by Arnaldo Videira

Research paper thumbnail of The internal alternative NADH dehydrogenase of Neurospora crassa mitochondria

Biochemical Journal, 2003

An open reading frame homologous with genes of non-proton-pumping NADH dehydrogenases was identif... more An open reading frame homologous with genes of non-proton-pumping NADH dehydrogenases was identified in the genome of Neurospora crassa. The 57 kDa NADH:ubiquinone oxidoreductase acts as internal (alternative) respiratory NADH dehydrogenase (NDI1) in the fungal ...

Research paper thumbnail of Composition of complex I from Neurospora crassa and disruption of two “accessory” subunits

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2005

Respiratory chain complex I of the fungus Neurospora crassa contains at least 39 polypeptide subu... more Respiratory chain complex I of the fungus Neurospora crassa contains at least 39 polypeptide subunits, of which 35 are conserved in mammals. The 11.5 kDa and 14 kDa proteins, homologues of bovine IP15 and B16.6, respectively, are conserved among eukaryotes and belong to the membrane domain of the fungal enzyme. The corresponding genes were separately inactivated by repeat-induced pointmutations, and null-mutant strains of the fungus were isolated. The lack of either subunit leads to the accumulation of distinct intermediates of the membrane arm of complex I. In addition, the peripheral arm of the enzyme seems to be formed in mutant nuo14 but, interestingly, not in mutant nuo11.5. These results and the analysis of enzymatic activities of mutant mitochondria indicate that both polypeptides are required for complex I assembly and function. D

Research paper thumbnail of The internal alternative NADH dehydrogenase of Neurospora crassa mitochondria

Biochemical Journal, 2003

An open reading frame homologous with genes of non-proton-pumping NADH dehydrogenases was identif... more An open reading frame homologous with genes of non-proton-pumping NADH dehydrogenases was identified in the genome of Neurospora crassa. The 57 kDa NADH:ubiquinone oxidoreductase acts as internal (alternative) respiratory NADH dehydrogenase (NDI1) in the fungal ...

Research paper thumbnail of Composition of complex I from Neurospora crassa and disruption of two “accessory” subunits

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2005

Respiratory chain complex I of the fungus Neurospora crassa contains at least 39 polypeptide subu... more Respiratory chain complex I of the fungus Neurospora crassa contains at least 39 polypeptide subunits, of which 35 are conserved in mammals. The 11.5 kDa and 14 kDa proteins, homologues of bovine IP15 and B16.6, respectively, are conserved among eukaryotes and belong to the membrane domain of the fungal enzyme. The corresponding genes were separately inactivated by repeat-induced pointmutations, and null-mutant strains of the fungus were isolated. The lack of either subunit leads to the accumulation of distinct intermediates of the membrane arm of complex I. In addition, the peripheral arm of the enzyme seems to be formed in mutant nuo14 but, interestingly, not in mutant nuo11.5. These results and the analysis of enzymatic activities of mutant mitochondria indicate that both polypeptides are required for complex I assembly and function. D

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