Arndt Guentsch - Academia.edu (original) (raw)

Papers by Arndt Guentsch

Diagnostic microbiology and infectious disease, 2011

The impact of a semiquantitative commercially available test based on DNA-strip technology (micro... more The impact of a semiquantitative commercially available test based on DNA-strip technology (microIDent®, Hain Lifescience, Nehren, Germany) on diagnosis and treatment of severe chronic periodontitis of 25 periodontitis patients was evaluated in comparison with a quantitative in-house real-time PCR. Subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. After extracting DNA, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and several other periodontopathogens were determined by both methods. The results obtained by DNA-strip technology were analyzed semiquantitatively and additionally quantitatively by densitometry. The results for the 4 major periodontopathogenic bacterial species correlated significantly between the 2 methods. Samples detecting a high bacterial load by one method and negative by the other were always found in less than 2% of the total samples. Both technologies showe...

ZWR - Das Deutsche Zahnärzteblatt, 2010

Journal of endodontics, 2013

To ensure root canal treatment success, endodontic microbiota should be efficiently reduced. The ... more To ensure root canal treatment success, endodontic microbiota should be efficiently reduced. The in vitro bactericidal effects of a hydrodynamic system and a passive ultrasonic irrigation system were compared. Single-rooted extracted teeth (n = 250) were contaminated with suspensions of Enterococcus faecalis ATCC 29212, mixed aerobic cultures, or mixed anaerobic cultures. First, the antibacterial effects of the hydrodynamic system (RinsEndo), a passive ultrasonic irrigation system (Piezo smart), and manual rinsing with 0.9% NaCl (the control) were compared. Colony-forming units were counted. Second, the 2 systems were used with 1.5% sodium hypochlorite (NaOCl) alone or NaOCl + 0.2% chlorhexidine (CHX). The colony-forming units in the treated and untreated roots were determined during a period of 5 days. Both irrigation systems reduced bacterial numbers more effectively than manual rinsing (P < .001). With NaCl, ultrasonic activated irrigation reduced bacterial counts significantl...

Quintessence international (Berlin, Germany : 1985), 2012

Periodontitis is an infection that results from an imbalance between periodontopathic microorgani... more Periodontitis is an infection that results from an imbalance between periodontopathic microorganisms and the local and systemic host defense. This study analyzed saliva samples of patients with periodontitis for several biomarkers of host response. Saliva was collected from 13 patients with chronic periodontitis, seven patients with aggressive periodontitis, and 13 periodontally healthy control subjects. Diverse markers of host response representing innate and adaptive immune response as well as antioxidative variables were determined. Patients with aggressive periodontitis had significantly higher values of lipid peroxidation and cathepsin C activity in saliva. The highest activities of neutrophil elastase, proteinase 3, and superoxide dismutase were measured in chronic periodontitis patients. Levels of antimicrobial peptides HNPs 1-3 were significantly highest in chronic periodontitis patients than in aggressive periodontitis or control subjects. Immunoglobulin G levels directed a...

Oral Microbiology and Immunology, 2008

$Research paper thumbnail of The isolated orbital floor fracture from a transconjunctival or subciliary perspective-A standardized anthropometric evaluation$

Medicina oral, patología oral y cirugía bucal, 2016

The influence of orbital fractures and their repair on the rate of deformities of the lower eyeli... more The influence of orbital fractures and their repair on the rate of deformities of the lower eyelid is an ongoing source of discussion in the literature. Most of the present studies include isolated blow-out as well as combined orbital fractures. We present a retrospective evaluation of a series of 100 patients after isolated blow-out fracture repair using reference anthropometric data on standardized photographs. Analysis included eye fissure width and height, lid sulcus height, upper lid height, upper and lower iris coverage, position of cornea to palpebra inferior, canthal tilt, scleral show, ectropion and entropion. It was clearly distinguished between operated and contralateral eyelid, whether a transconjunctival or a subciliary approach was performed and amount of fracture. Our main interests were changes of the aforementioned parameters with regards to eyelid deformities. Surgery per se did not significantly influence eyelid deformities. However, the surgical approach selected...

Clinical oral investigations, Jan 2, 2016

Postoperative pain management is of highest interest for patients undergoing maxillofacial surger... more Postoperative pain management is of highest interest for patients undergoing maxillofacial surgery including microvascular reconstructive surgery. Currently, there is a lack of information regarding process and outcome of postoperative pain management after microvascular reconstruction. In a prospective clinical study, 31 adults were evaluated on the first postoperative day following microvascular reconstruction with a radial forearm flap using the standardized questionnaire of the Germany-wide project Quality Improvement in Postoperative Pain Management (QUIPS). It enables a standardized assessment of patients' characteristics, pain parameters, outcome and pain therapy process parameters. Pain management consisted predominately of premedication with midazolam, sufentanil and metamizol intraoperatively, piritramid in the intensive care unit and metamizol, tramadol and fentanyl patches on ward. Nineteen patients (61.3 %) showed inadequate pain management with pain levels ≥4. Amon...

Clinical Oral Investigations, 2016

The duration and the frequency of follow-up after treatment of oral squamous cell carcinoma are n... more The duration and the frequency of follow-up after treatment of oral squamous cell carcinoma are not standardized in the current literature. The purpose of this study was to evaluate our local standard post-treatment and follow-up protocol. Overall, 228 patients treated curatively from 01/2006 to 07/2013 were reviewed. To evaluate the follow-up program, data on the secondary event were used. To determine risk groups, all patients with tumor recurrence were specifically analyzed. Relapse-free rate were estimated by the Kaplan-Meier product limit method. The chi-square test was used to identify independent risk factors for tumor relapse. In total, 29.8 % patients had a secondary event. The majority of the relapse cases (88.2 %) were detected within 2 years postoperatively, 61.8 % of them within the first year. Most events were local recurrences (34.7 %). UICC-stage IV was significantly associated with tumor recurrence (p = 0.001). Gender (p = 0.188), age (p = 0.195), localization (p = 0.739), T-stage (p = 0.35), N-stage (p = 0.55), histologic grade (p = 0.162), and tobacco and alcohol use (p = 0.248) were not significantly associated with tumor recurrence. Patients with positive neck nodes relapsed earlier (p = 0.011). The majority of relapses (86.3 %) were found in asymptomatic patients at routine follow-up. The results of this study suggest an intensified follow-up within the first 2 years after surgery. Given the higher relapse rate of patients exhibiting an UICC-stage IV and/or positive neck nodes, it seems to be from special interest to perform in this group a risk-adapted follow-up with monthly examinations also in the second year.

Objective: To compare the effects of oral prophylaxis on immunological and microbiological parame... more Objective: To compare the effects of oral prophylaxis on immunological and microbiological parameters in subjects without clinical signs of periodontitis and patients with chronic periodontitis. Methods: 30 subjects were included in this trial (each n = 15 patients with generalized chronic periodontitis and clinically periodontal healthy controls (no attachment loss)). Immediately before and 14 days after an oral prophylaxis session subgingival plaque as well as GCF were collected. The major periodontopathogens were determined by a PCR-based method. In addition, levels of IL-1beta, IL-8, IL-10 and MMP-8 were measured. Statistical analysis was performed using ANOVA. Results: At both time points, the ratio between the pro-inflammatory cytokine IL-1beta and the anti-inflammatory cytokine IL-10 was significantly higher in periodontitis patients (p < 0.002). At baseline, only the level of IL-10 was significantly different between both groups (p < 0.05). However, after oral prophyla...

Objective: Patients with periodontal diseases frequently suffer from pathological malodor caused ... more Objective: Patients with periodontal diseases frequently suffer from pathological malodor caused mainly by volatile sulfur compounds (VSC). A clinical trial was designed to evaluate the effects of professional teeth and tongue cleaning on halitosis-associated parameters. Method: In sum 30 subjects were included in this longitudinal cohort study (each n=15 patients with generalized chronic periodontitis and periodontal healthy controls). VSC-values (Halimeter) and organoleptic scores (grade 0 = no odor until 5 = extreme odor measured from distances of 10, 30 and 100 cm) as well as the tongue coating index were evaluated before and 14 days after of professional teeth cleaning with ultrasonic instruments and tongue cleaning with a tongue cleaner. The VSC were also measured immediately after treatment. Data were statistical analyzed with ANOVA and paired t-test. Result: Supra- and subgingival plaque and calculus removal with combined tongue cleaning was able to reduce the VSC-values in ...

Objectives: The objective of this in vitro study was to measure the trueness and precision of a n... more Objectives: The objective of this in vitro study was to measure the trueness and precision of a newly-developed intraoral scanner and compare its results to a common plaster cast digitizer. Methods: An acrylic model of a lower left first molar was prepared to receive a ceramic partial crown and was duplicated by a digital-impressioning-system (cara TRIOS, Heraeus Kulzer GmbH, Hanau, Germany - TRI) and conventional one-step dual-viscosity impressions (Identium, Kettenbach GmbH, Eschenburg, Germany - IDE) as well. TRI-data were exported directly to a common quality-inspection-software, while in case of IDE, plaster models were cast and laser-scanned (7 Series, Dental Wings, Montreal, Canada), before datasets were subjectable to further computer-aided analyses. The model tooth was digitized by a structure-light-scanner to serve as a reference for trueness measurements (tooth vs impression), while overall precision was determined by superimposing one and another of the impressions. Mean...

Objectives: CNC-milled restorations have been investigated for their preciseness before, while de... more Objectives: CNC-milled restorations have been investigated for their preciseness before, while detailed information on the milling-step itself are lacking. The authors conducted a study to investigate the influence of CNC-milling on spatial adaption of ceramic restorations. Methods: An acrylic model of a lower left first molar was prepared to receive a ceramic partial crown and was duplicated by one step dual viscosity impressions. Gypsum casts were formed and laser-scanned to realize virtual datasets, before restorations were designed, exported(pre-milling-data=PRE) and CNC-machined from lithiumdisilicate-blanks. Crowns were digitized by a structure-light-scanner to obtain post-milling-data(=POST). PRE and POST were virtually superimposed on the reference tooth and subjected to computer-aided-inspection. Visual fit-discrepancies were displayed with colors, while, mean quadratic deviations(RMS) were computed and analyzed by a Student t-test(n=5, α=0.05). Results: Mean(SD) RMS-values...

Objectives: The marginal and internal fit of ceramic restorations have been described in 1 or 2 d... more Objectives: The marginal and internal fit of ceramic restorations have been described in 1 or 2 dimensions, whereas it is most desirable to evaluate adaption spatially, in 3 dimensions. The authors conducted a study to investigate the influence of digital and conventional impressions on the spatial fit of ceramic partial crowns (CPCs). Methods: An acrylic model of a lower left first molar was prepared to receive a CPC and was duplicated by 2 digital(LAV, ITE) and 1 conventional(IDE) impression systems. LAV- and ITE-impressions were exported directly to a common CAD-software, while in case of IDE, plaster models were cast and laser-scanned to realize virtual datasets. CPCs were designed and machined from prefabricated lithiumdisilicate-blanks. The tooth model and the machined ceramics were digitized by a structure-light-scanner, virtually superimposed one on the other and subjected to computer-aided-inspection. Mean quadratic deviations(RMS) were computed separately for marginal and ...

Objective: In periodontitis, an effective host-response is primarily related to PMNs, which inclu... more Objective: In periodontitis, an effective host-response is primarily related to PMNs, which includes the release of active neutrophil elastase (NE) and proteinase 3 (PR3). It was shown that proteases (Arg-gingipains) of one of the major periodontopathogens, Porphyromonas gingivalis (P.g.) degrade Elafin (Ela), an inhibitor of NE in vitro. The purpose of this pilot-study was to determine Ela in relation to P.g. in gingival crevicular fluid (GCF). Methods: GCF of 31 subjects (9 healthy controls (CON), 7 gingivitis (GIN), 5 aggressive (AP) and 10 chronic periodontitis (CP)) was analyzed by Western Blot for Ela. Furthermore, activities of NE, PR3 and loads of P.g., incl. its Arg-specific proteolytic activity (BApNA), Aggregatibacter actinomycetemcomitans (A.a.), Tannerella forsythia (T.f.) and Treponema denticola (T.d.) were determined. Results: The amount of Ela was different between all subject-groups (Kruskal-Wallis-Test, p=0.001). The highest values were found in AP and lowest value...

ZWR - Das Deutsche Zahnärzteblatt, 2012

International journal of dental hygiene, 2014

A controlled clinical trial was conducted to evaluate the effects of oral prophylaxis on halitosi... more A controlled clinical trial was conducted to evaluate the effects of oral prophylaxis on halitosis-associated, immunological and microbiological parameters. Thirty subjects were included in this controlled clinical trial (patients with generalized chronic periodontitis and controls without clinical attachment loss; each n = 15). Before oral prophylaxis and 14 days after (including tongue cleaning) volatile sulphur compounds (VSC), organoleptic scores and a tongue coating index were evaluated. The levels of IL-1β, IL-8, IL-10 and MMP-8 were measured in GCF, and also major periodontal pathogens were detected. Data were statistically analysed using anova and paired t-test. Supragingival plaque and calculus removal with combined tongue cleaning was able to reduce significantly (P < 0.05) the VSC values in both groups (no significant differences between both groups). Two weeks after periodontal debridement, the VSC values were observed in the periodontitis group, but not in the contro...