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Papers by Arnulfo Castro

Research paper thumbnail of Cytochrome b for identification of animal species in processed food

International Congress Series, 2004

The objective of this work has been to develop a suitable methodology based on mitochondrial cyto... more The objective of this work has been to develop a suitable methodology based on mitochondrial cytochrome b gene (cytb) to allow the identification of animals species used as raw material in the manufacture of processed food. In some cases, more detailed information on the kind of food sample found in the stomach of a victim may be of particular importance. A number of specimens of processed food with bovine, pork and chicken origin were analyzed. DNA was extracted by two methods: (1) Cell lysis using proteinase K and SDS, and phenol-chloroform DNA purification; (2) NucleoSpin Food (Macherey-Nagelk). PCR amplification of 358 pb of the cytb was done. The species of the samples were identified by aligning to the cytb gene sequence entries using BLAST of the National Center for Biotechnology Information. Both the DNA extraction procedures yielded enough DNA to be analyzed by PCR. Sequences obtained were searched against nucleotide sequence database (GenBank). With this procedure, it is possible to classify each sample correctly according to its biological species. D

Research paper thumbnail of Molecular identification of arthropods by cytochrome b analysis

International Congress Series, 2004

This work has been developed to improve the analysis of cadaveric entomofauna. Taking into accoun... more This work has been developed to improve the analysis of cadaveric entomofauna. Taking into account that the cytochrome b is widely use to identify vertebrates, we have used it for the identification of some necrophagous species involved in the postmortem process. D 2004 Published by Elsevier B.V.

Research paper thumbnail of Cytochrome b and HVI sequences of mitochondrial DNA to identify domestic animal hair in forensic casework

International Congress Series, 2003

Research paper thumbnail of Seven SNPs and YAP demonstrate that the chromosome Y lineages of Basques are different from Georgians and Berbers

International Congress Series, 2003

Research paper thumbnail of Genetic structure of autochthonous Basques through analysis of the HVI and HVII regions of mitochondrial DNA

International Congress Series, 2003

Research paper thumbnail of Y chromosome STRs in the north of Portugal

International Congress Series, 2004

The interest in Y chromosome polymorphisms has continuously increased, not only in evolutionary/g... more The interest in Y chromosome polymorphisms has continuously increased, not only in evolutionary/genealogical studies, but also in forensic genetics. The objective of this study is to analyse the loci DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393 and DYS385I/II in a population sample from the North of Portugal. One hundred one unique haplotypes were found and the haplotypic diversity was 0.998 F 0.001 which reinforces the forensic utility of these markers. Phylogenetic analysis shows that our population can be included in the European Y chromosome lineages and distinct from the Asian ones. D

Research paper thumbnail of Analysis of the HVI and HVII regions of mitochondrial DNA in 100 individuals from North of Portugal

International Congress Series, 2004

The analysis of mitochondrial DNA (mtDNA) control region is of great importance in forensic casew... more The analysis of mitochondrial DNA (mtDNA) control region is of great importance in forensic casework and evolution studies. The aim of this study was to create a population database for the HVI and HVII regions of mtDNA in the population of North of Portugal. The HVI and HVII segments were studied in 100 unrelated and healthy individuals by direct sequencing. Nucleotide substitutions were found using Anderson's reference sequence. Length and position heteroplasmy were observed. The genetic structure of the population was analysed. The match probability and discrimination power values were calculated. The phylogenetic analysis for HVI segment was made following classification into haplogroups and genetic distances were calculated. These analyses place the North of Portugal population among the European Caucasoid populations. D

Research paper thumbnail of Switching from tipranavir (TPV) 500/ritonavir (RTV) 200 mg to TPV 500/RTV 100 mg in treatment-experienced patients (pts) with HIV RNA

Journal of The International Aids Society, 2010

Research paper thumbnail of Exposition and sequencing to antiretrovirals in HIV1 patients with triple-class antiretroviral failure, and harbouring protease resistance mutations

Journal of The International Aids Society, 2008

Research paper thumbnail of Cardiovascular risk estimation in Spanish HIV-infected patients: a multicenter cohort study

Journal of The International Aids Society, 2008

Research paper thumbnail of Cytochrome b for identification of animal species in processed food

International Congress Series, 2004

The objective of this work has been to develop a suitable methodology based on mitochondrial cyto... more The objective of this work has been to develop a suitable methodology based on mitochondrial cytochrome b gene (cytb) to allow the identification of animals species used as raw material in the manufacture of processed food. In some cases, more detailed information on the kind of food sample found in the stomach of a victim may be of particular importance. A number of specimens of processed food with bovine, pork and chicken origin were analyzed. DNA was extracted by two methods: (1) Cell lysis using proteinase K and SDS, and phenol-chloroform DNA purification; (2) NucleoSpin Food (Macherey-Nagelk). PCR amplification of 358 pb of the cytb was done. The species of the samples were identified by aligning to the cytb gene sequence entries using BLAST of the National Center for Biotechnology Information. Both the DNA extraction procedures yielded enough DNA to be analyzed by PCR. Sequences obtained were searched against nucleotide sequence database (GenBank). With this procedure, it is possible to classify each sample correctly according to its biological species. D

Research paper thumbnail of Molecular identification of arthropods by cytochrome b analysis

International Congress Series, 2004

This work has been developed to improve the analysis of cadaveric entomofauna. Taking into accoun... more This work has been developed to improve the analysis of cadaveric entomofauna. Taking into account that the cytochrome b is widely use to identify vertebrates, we have used it for the identification of some necrophagous species involved in the postmortem process. D 2004 Published by Elsevier B.V.

Research paper thumbnail of Cytochrome b and HVI sequences of mitochondrial DNA to identify domestic animal hair in forensic casework

International Congress Series, 2003

Research paper thumbnail of Seven SNPs and YAP demonstrate that the chromosome Y lineages of Basques are different from Georgians and Berbers

International Congress Series, 2003

Research paper thumbnail of Genetic structure of autochthonous Basques through analysis of the HVI and HVII regions of mitochondrial DNA

International Congress Series, 2003

Research paper thumbnail of Y chromosome STRs in the north of Portugal

International Congress Series, 2004

The interest in Y chromosome polymorphisms has continuously increased, not only in evolutionary/g... more The interest in Y chromosome polymorphisms has continuously increased, not only in evolutionary/genealogical studies, but also in forensic genetics. The objective of this study is to analyse the loci DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393 and DYS385I/II in a population sample from the North of Portugal. One hundred one unique haplotypes were found and the haplotypic diversity was 0.998 F 0.001 which reinforces the forensic utility of these markers. Phylogenetic analysis shows that our population can be included in the European Y chromosome lineages and distinct from the Asian ones. D

Research paper thumbnail of Analysis of the HVI and HVII regions of mitochondrial DNA in 100 individuals from North of Portugal

International Congress Series, 2004

The analysis of mitochondrial DNA (mtDNA) control region is of great importance in forensic casew... more The analysis of mitochondrial DNA (mtDNA) control region is of great importance in forensic casework and evolution studies. The aim of this study was to create a population database for the HVI and HVII regions of mtDNA in the population of North of Portugal. The HVI and HVII segments were studied in 100 unrelated and healthy individuals by direct sequencing. Nucleotide substitutions were found using Anderson's reference sequence. Length and position heteroplasmy were observed. The genetic structure of the population was analysed. The match probability and discrimination power values were calculated. The phylogenetic analysis for HVI segment was made following classification into haplogroups and genetic distances were calculated. These analyses place the North of Portugal population among the European Caucasoid populations. D

Research paper thumbnail of Switching from tipranavir (TPV) 500/ritonavir (RTV) 200 mg to TPV 500/RTV 100 mg in treatment-experienced patients (pts) with HIV RNA

Journal of The International Aids Society, 2010

Research paper thumbnail of Exposition and sequencing to antiretrovirals in HIV1 patients with triple-class antiretroviral failure, and harbouring protease resistance mutations

Journal of The International Aids Society, 2008

Research paper thumbnail of Cardiovascular risk estimation in Spanish HIV-infected patients: a multicenter cohort study

Journal of The International Aids Society, 2008

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