Aroonsri Priprem - Academia.edu (original) (raw)

Papers by Aroonsri Priprem

International Journal of Advances in Agricultural and Environmental Engineering, 2016

Crops of waxy purple corn (Zea mays L. ceritina Kulesh., PC), a hybrid corn variety rich in antho... more Crops of waxy purple corn (Zea mays L. ceritina Kulesh., PC), a hybrid corn variety rich in anthocyanin contents, provide abundant waste byproducts from its cobs (PCC), silks (PCS), husks (PCH), and pollens (PCP), of which phytochemicals and antioxidative activities being determined. Each byproduct was be subjected to extracted by maceration and separation from various solvents. All PC byproducts contained carbohydrates, tannins, flavonoids, and anthocyanins. Thin layer chromatography reported cyanidin-3-glucoside (C3G) as the major anthocyanins of PCC and PCH. Hydro-ethanolic extracts (HE) of PCS gave the highest level of phenolic compound of 357 mg GAE/g, while that of PCH gave the highest anthocyanin contents of 0.11 mg C3G/ g. DPPH, ABTS, and FRAP assays were used to compare all extracts of PC byproducts. HE of PCH and PCS provided the best ABTS values of 328 and 328 mg TEAC/g, respectively, and also the strongest DPPH radical scavenging of 365 mg TEAC/g. Aqueous extracts of PCC gave the highest FRAP value with 800 mg Fe(II)/g. It is concluded that these byproducts of PC could provide high yield of valuable phytochemicals with antioxidative potentials for use in health products.

AAPS PharmSciTech

Mucositis is one of the most adverse effects of 5-fluorouracil (5-FU) and had no standard drug fo... more Mucositis is one of the most adverse effects of 5-fluorouracil (5-FU) and had no standard drug for treatment. Melatonin is a neurohormone, and can ameliorate radiotherapy-induced small intestinal mucositis. Melatonin encapsulated in niosomes improved its poor bioavailability. Succinyl melatonin, a melatonin derivative, showed prolonged release compared with melatonin. This study investigated the efficacy of melatonin niosome gel (MNG) and succinyl melatonin niosome gel (SNG) in 5-FU-induced small intestinal mucositis treatment in mice. MNG and SNG with particle sizes of 293 and 270 nm were shown to have mucoadhesive potentials. The effect of a daily oral application of MNG, SNG, or fluocinolone acetonide gel (FAG, positive control) was compared to that of the normal group. The body weight, food consumption, histology, Fourier transform infrared (FTIR) spectroscopy, inflammatory cytokines (tumor necrosis factor (TNF)-α and interleukin (IL)-1β), and malondialdehyde (MDA) in the small intestine were monitored. The results showed decreased %body weight and food consumption in all 5-FU-injected groups compared with the normal group. The MNG and SNG treatments maintained the food consumption and the normal integrity of the small intestines, as evidenced by villus length and crypt depth, similar to the observations in the normal groups. The FTIR spectra showed no change in lipids of the MNG and SNG groups compared with the normal group. Moreover, SNG could reduce IL-1β content to a level that was not different from the level in the normal groups. Therefore, the oral application of MNG and SNG could protect against 5-FU-induced small intestinal mucositis in mice.

Archives of Pharmacy Practice, 2012

Asia-Pacific Journal of Science and Technology, 2007

Certain trace metals in fish may lead to health problems through the food chain. This work aimed ... more Certain trace metals in fish may lead to health problems through the food chain. This work aimed to study the accumulation of some heavy metals in freshwater fish along the Pong and Chi rivers. The fish were randomly sampled during late 2002 until early 2003 from 8 monitoring sites, marked A, B, C, D, E, F, G, H, located downstream between Ubolratana reservoir and Mahasarakham weir covering a river length of about 180 km. Cd, Co, Cr, Cu, Ni, Pb and Zn, were determined from samples of the muscle and adipose tissue of the fish by using atomic absorption. In comparison, the levels of heavy metals in the adipose were higher than those found in the muscle of the fish. The mean concentrations in these tissues were listed from high to low levels as follows: Zn > Cu > Cr > Co > Ni > Pb> Cd. Sites B and H showed high potential bioaccumulation of heavy metals in these fish during the highly contaminated season. Pollutant stress in fish should thus be emphasized with an estab...

Materials Today Communications, 2021

Abstract Curcumin-loaded nanocarriers clustered into hollow porous microspheres composed of arabi... more Abstract Curcumin-loaded nanocarriers clustered into hollow porous microspheres composed of arabic gum (AG) and xanthan gum (XG) form a solid dispersion, so-called nanoencapsulated curcumin (NEC), were investigated. In vitro study shows little or no release of curcumin from NEC into a buffer at pH 1.2, whereas a release rate of 1.4 %/h was obtained from that at pH 6.8 after 6 h, indicating intestinal absorption. In vivo evaluation in hamsters presented a peak plasma curcumin level of 400 ng/mL at 24 h after oral administration indicated an extended release of curcumin. Mucoadhesive NEC was rarely observed in stomach tissues but mostly in intestine after 72 h consumption, resulting in curcumin was detectable in plasma up to 3 days. NEC was not cytotoxic to normal fibroblasts but cytotoxic to cholangiocarcinoma cell line, suggesting anti-cholangiocarcinoma potentials. Under normal storage conditions for 3 years, up to 97 % of curcumin could be found in NEC, suggesting long-term stability. Therefore, NEC, a solid dispersion of curcumin-loaded nanocarriers in AG/XG gums, prolonged release with mucoadhesion providing enhanced intestinal absorption of curcumin and indicating cholangiocarcinoma potentials.

Polymers, 2021

Chitosan or polyvinyl pyrrolidone (PVP) were used in combination with hydroxypropyl methylcellulo... more Chitosan or polyvinyl pyrrolidone (PVP) were used in combination with hydroxypropyl methylcellulose (HPMC) and poloxamer 407 (P407) as gelling agents for oral drug delivery. The performance interaction with mucin of chitosan-composed gel (F1) and PVP-composed gel (F2) was compared using attenuated total reflectance–Fourier-transform infrared (ATR-FTIR) spectroscopy at controlled temperatures of 25 and 37 °C for 1 and 5 min. F1 containing niosome-entrapped melatonin or its derivatives was investigated for mucoadhesive interaction on mucosa by ATR-FTIR spectroscopy under the same conditions. The results showed that F1-treated mucin gave a significantly lower amide I/amide II ratio than untreated mucin and F2-treated mucin did within 1 min, suggesting improved rapid affinity between mucin and chitosan. The spectra of mucosa treated with F1 incorporating niosomes of melatonin or its derivatives showed peak shifts at C=O (amide I), N-H (amide II), and carbohydrate regions and an associat...

Molecules, 2021

This study focuses on the role of photosensitizers in photodynamic therapy. The photosensitizers ... more This study focuses on the role of photosensitizers in photodynamic therapy. The photosensitizers were prepared in combinations of 110/220 µM erythrosine and/or 10/20 µM demethoxy/bisdemethoxy curcumin with/without 10% (w/w) nano-titanium dioxide. Irradiation was performed with a dental blue light in the 395–480 nm wavelength range, with a power density of 3200 mW/cm2 and yield of 72 J/cm2. The production of ROS and hydroxyl radical was investigated using an electron paramagnetic resonance spectrometer for each individual photosensitizer or in photosensitizer combinations. Subsequently, a PrestoBlue® toxicity test of the gingival fibroblast cells was performed at 6 and 24 h on the eight highest ROS-generating photosensitizers containing curcumin derivatives and erythrosine 220 µM. Finally, the antifungal ability of 22 test photosensitizers, Candida albicans (ATCC 10231), were cultured in biofilm form at 37 °C for 48 h, then the colonies were counted in colony-forming units (CFU/mL) v...

Key Engineering Materials, 2020

Complexation of anthocyanin has been introduced to help improved anthocyanin stability. Slightly ... more Complexation of anthocyanin has been introduced to help improved anthocyanin stability. Slightly water solubility, after forming complex of anthocyanins, represents obstacle against formulation preparation. The objective of this study is to formulate topical gel containing anthocyanin for treating oral wound. Niosomes were introduced to help delivery anthocyanins and increasing permeability. Development and evaluation of anthocyanin complex (AC) gel and anthocyanin complex niosomes (ACN) gel have been compared in physicochemical properties such as particle size, zeta potential, homogeneity, release and permeation of the gel including stability of the gel formulations. The average size of AC of ~5 nm was increased to ~100 nm after encapsulated in niosomal vesicle. Similar to the particle size, zeta potential of ACN showed greater negatively charge than the AC. The cell viability of human gingival fibroblast (HGF) exposed to AC and ACN in a concentration of 2-2000 µg/ml for 24 h showe...

Photodiagnosis and Photodynamic Therapy, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

日本環境変異原学会大会プログラム要旨集, Nov 10, 2007

Polymers

This study used polymeric micelles to improve quality by increasing drug solubility, extending mu... more This study used polymeric micelles to improve quality by increasing drug solubility, extending mucosal drug retention time, enhancing mucoadhesiveness, and promoting drug permeation and deposition. Fluocinolone acetonide (FA) was loaded into polymeric micelles (FPM), which were composed of poloxamer 407 (P407), sodium polyacrylate (SPA), and polyethylene glycol 400, and their physicochemical properties were examined. Small-angle X-ray scattering (SAXS) revealed a hexagonal micellar structure at all temperatures, and the concentrations of P407 and SPA were shown to significantly affect the solubility, mucoadhesion, release, and permeation of FPMs. The proportion of P407 to PEG at a ratio of 7.5:15 with or without 0.1% w/v of SPA provided suitable FPM formulations. Moreover, the characteristics of FPM7 and FPM8 revealed crystalline states inside the micelles, which was consistent with the morphology and nano-hexagonal structure. The results of ex vivo experiments using focal plane arr...

Pharmaceutics, 2022

Sericin-alginate hydrogel formulations with purple waxy corn (Zea mays L.) cob extract (PWCC) for... more Sericin-alginate hydrogel formulations with purple waxy corn (Zea mays L.) cob extract (PWCC) for topical anti-inflammatory application are developed and evaluated. The physical properties such as viscosity, pH, and anthocyanin release are examined and in vitro anti-inflammatory activities, such as NO inhibition and IL-6, IL-1β, TNF-α, iNOS, and COX-2 expression, are evaluated in LPS-stimulated RAW 264.7 murine macrophages. The sericin-alginate hydrogel is prepared by physical crosslinking through the ionic interaction of the polymers combined with anthocyanin from PWCC at pH 6.5. The hydrogel formulation with 2.00% w/v sericin, 0.20% w/v alginate, and 0.15% w/v PWCC (SN6) shows a suitable viscosity for topical treatment, the highest nitric oxide inhibition (79.43%), no cytotoxicity, and reduced expression of IL-6, IL-1β, and TNF-α mediators. Moreover, the SN6 formulation displays a sustained anthocyanin release over 8–12 h, which correlates with the Korsmeyer–Peppas model. The FT-I...

Complexation of anthocyanins to mimic natural copigmentation process was investigated. ... more Complexation of anthocyanins to mimic natural copigmentation process was investigated. Cyanidin-rich extracts from Zea mays L. ceritina Kulesh. and delphinidin-rich extracts from Clitoria ternatea L. were used to form 4 anthocyanin complexes, AC1, AC2, AC3 and AC4, in the presence of several polyphenols and a trace metal. Characterizations of the ACs were conducted by UV, FTIR, DSC/TGA and morphological observations. Bathochromic shifts of the UV spectra of 4 formulas of ACs were observed at peak wavelengths of about 510-620 nm by 10 nm suggesting complex formation. FTIR spectra of the ACs indicate shifts of peaks from 1,733 cm-1 to 1,696 cm-1 indicating interactions and a decrease in the peak areas within the wavenumber of 3,400-3,500 cm-1 indicating changes in hydrogen bonding. Thermal analysis of all of the ACs suggests increases in melting temperature...

Anthocyanins are natural pigments with effective UV protection but their topical use co... more Anthocyanins are natural pigments with effective UV protection but their topical use could be limited due to their physicochemical characteristics. An attempt to overcome such limitations by complexation of 2 major anthocyanin-rich sources, C. ternatea and Z. mays, has potentiated its use as topical antiinflammatory. Cell studies indicate no cytotoxicity of the anthocyanin complex (AC) up to 1 mg/ml tested in HaCaT and human fore head fibroblasts by MTT. Croton oil-induced ear edema in Wistar rats suggests an effective dose of 5 mg/cm2 of AC as a topical anti-inflammatory in comparison to 0.5 mg/cm2 of fluocinolone acetonide. Niosomal encapsulation of the AC significantly prolonged the anti-inflammatory activity particularly at 8 h after topical application (p = 0.0001). The AC was not cytotoxic and its anti-inflammatory and activity was dose-dependent an...

Supplemental material, Supplemental data for Effects of Anthocyanins and Melatonin From Purple Wa... more Supplemental material, Supplemental data for Effects of Anthocyanins and Melatonin From Purple Waxy Corn By-Products on Collagen Production by Cultured Human Fibroblasts by Thithima Rimdusit, Suthasinee Thapphasaraphong, Ploenthip Puthongking, and Aroonsri Priprem in Natural Product Communications

Background and objective: Leaves of Clinacanthus nutans (CN) is one of the most well-known herbal... more Background and objective: Leaves of Clinacanthus nutans (CN) is one of the most well-known herbal plants listed in Thai Herbal Pharmacopoeia, exerting anti-inflammatory and antiviral activities. One of its use which is currently stated in the Thai national list of essential medicine (NLEM) is topical oral applications for mouth sore from aphthous ulcer, chemotherapy and radiotherapy-induced oral ulcer or herpetic gingivostomatitis. Unacceptable appearance, odor and taste of the current hospital formula of the CN topical oral solution, listed in NLEM, indicate a need to revise the formulation. Mucoadhesive polymers are shown to improve the quality and functions of topical oral preparations. This study intended to improve the CN topical oral solution using a mucoadhesive polymer and compare with the current hospital formula. The modified formula would be registered as the hospital formula. This study aimed to modify oral bases intended to improve CN topical oral solution for use as...

Background: This study aims to investigate the stability of Zanthoxylum limonella Alston. seed (Z... more Background: This study aims to investigate the stability of Zanthoxylum limonella Alston. seed (ZL) at various storage temperatures and anti-inflammatory activity of the topical oral gel (ZG) in a preclinical study. Methods: The seed was stored in 2 different conditions: accelerated condition (50 °C, 70 °C and 90 °C) over 96 h and long-term condition (4 °C, 25 °C and 50 °C) over 6 months. Anti-inflammatory activity of the topical oral in rats was observed by using croton oil ear edema and oral punch wound healing. Results: High storage temperature decreased the total phenolic content of ZL extract which the rate of degradation followed zero-order kinetic. In the long term condition, ZL stored at 50°C generated free radical while at 4 °C and 25 °C found the non-significant difference from the initial. The rate of release and permeation of phenolic content detected in receptor medium of the ZL into a mucoadhesive gel was 0.11 and 0.47 µg/cm2/h with no lag time indicated the fast relea...

Fruits of Zanthozylum limonella Alston, one of the spices traditionally used for toothache relief... more Fruits of Zanthozylum limonella Alston, one of the spices traditionally used for toothache relief, were hydro-distilled to obtain about 4.3% yield of volatile oil (ZL). 30% of ZL in a bioadhesive gel was prepared for in vitro permeation and in vivo tests. In vitro permeation of total phenolic contents of the ZL gel through porcine esophagus, as the model mucous membrane, using Franz diffusion cells indicates an average steady-state rate of 0.1 ± 0.02 mgGAE/cm2/h at 37 C (n = 5) without lag time. In vivo study in buccal pressure-wounds of rats indicates that licking behaviour was obvious and could be inhibited by gels containing xylocaine or ZL but not fluocinolone. Histological analysis of the inflammed cells in excised oral wounds showed that it took 1 day for fluocinolone gel to significantly reduce the number of inflammed cells and 2 days for ZL gel (p<0.05 both). Comparative wound assessments (edema and erythema) indicate mild anti-inflammatory activity of ZL gel. Thus, pheno...