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Papers by Ashkan Alamdary

Majallah-i mīkrub/shināsī-i pizishkī-i Īrān, Mar 18, 2024

Jundishapur journal of microbiology, Jan 17, 2024

Virus Research, Nov 1, 2020

Iranian Journal of Medical Microbiology

Background Rabies is a neurotropic virus that causes about 59000 deaths worldwide annually. The m... more Background Rabies is a neurotropic virus that causes about 59000 deaths worldwide annually. The most effective means to control and prevent rabies is prevention through proper pre- and post-exposure vaccination. Glycoprotein (G) is one of five structural proteins of the rabies virus and has a pivotal role in host immunity against the virus. This research has evaluated the results of incorporating an additional copy of the glycoprotein gene in the rabies virus genome on the immunogenicity and propagation rate of the recombinant virus. Methods . A PCR amplified copy of the G gene was previously inserted into the genome of the rabies virus PV strain. The recombinant virus glycoprotein expression was compared with the PV strain. The propagation rate of the recombinant virus in cell culture and its immunogenicity in BALB/c mice were assessed. The rapid fluorescent focus inhibition test (RFFIT) was used to analyze the virus-neutralizing antibodies (VNAs) in the mice sera. Results The addi...

Future Virology

The HCV Core protein is a multifunctional protein that interacts with many viral and cellular pro... more The HCV Core protein is a multifunctional protein that interacts with many viral and cellular proteins. In addition to the encapsidation of the viral genome, it can disturb various cellular pathways and impede antiviral cellular responses such as interferon (IFN) production. The Core protein can also disrupt the functions of immune cells against HCV. The Core protein helps viral infection persistency by interfering with apoptosis. The Core+1 protein plays a significant role in inducing chronic HCV infection through diverse mechanisms. We review some of the mechanisms by which Core and Core+1 proteins facilitate HCV infection to chronic infection. These proteins could be considered for designing more sufficient treatments and effective vaccines against HCV.

European Proceedings of Social and Behavioural Sciences, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution-No... more This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Vaccine Research

Introduction: Rabies is almost always fatal but entirely preventable through proper vaccination. ... more Introduction: Rabies is almost always fatal but entirely preventable through proper vaccination. Inadequacy of costly high-quality cell culture vaccines is sometimes a bottleneck for expanded rabies control plans. Reverse genetics along with other molecular biology means are trying to improve the immunogenicity and yield of rabies vaccine products. Methods: An additional glycoprotein gene of the rabies virus PV strain was inserted between the glycoprotein and polymerase genes of the virus. The viral proteins were expressed at the T7BHK cell line to rescue the recombinant virus. Results: The recombinant virus containing two consecutive glycoprotein genes was rescued from T7BHK cells. The virus particles were functional and successfully infected the permissive BSR cell line. Conclusion: The new virus strain with an additive copy of the glycoprotein gene has a good potential to be utilized in different studies, including cell biology and immunological properties of the rabies virus. In this study, the recombinant rabies virus was successfully rescued from cell culture which would pave the way for further investigations on this virus.

Archives of Iranian medicine, 2018

BACKGROUND This study aimed to evaluate Rabies virus vaccine strains. The obtained results may be... more BACKGROUND This study aimed to evaluate Rabies virus vaccine strains. The obtained results may be helpful for vaccine producers and researchers to compare the strains with wild type and other vaccine strains and select the correct strain to challenge their products. METHODS Fourteen rabies virus vaccine strains were compared with each other. The full genomes of the selected strains were taken from the GenBank and the N, P and G genes were labeled. The major and minor antigenic sites of these sequences were identified and contrasted with each other. The identity matrix was designed for rabies virus full genome, N and G genes. In addition, the phylogenetic tree was drawn based on rabies virus N gene for deep analysis. RESULTS Although there were no significant differences between antigenic sites in N, P, and G genes, there were noticeable differences for full genome identity matrix and this significant difference can also be observed in N and G identity matrix. In the phylogenetic tre...

Journal of Medical Virology

Earlier observation suggests that hepatitis C virus (HCV) is a single‐stranded RNA virus which en... more Earlier observation suggests that hepatitis C virus (HCV) is a single‐stranded RNA virus which encodes at least 10 viral proteins. F protein is a novel protein which has been discovered recently. These studies suggest three mechanisms for the production of this protein concerning ribosomal frameshift at codon 10, initial translation at codons 26 and 85 or 87. In this study, the association between protein F and chronicity of hepatocellular carcinoma (HCC) has been reviewed. Evidence suggests that humoral immune system can recognize this protein and produce antibodies against it. By detecting antibodies in infected people, investigators found that F protein might have a role in HCV infection causing chronic cirrhosis and HCC as higher prevalence was found in patients with mentioned complications. The increment of CD4+, CD25+, and FoxP3+ T cells, along with CD8+ T cells with low expression of granzyme B, also leads to weaker responses of the immune system which helps the infection to become chronic. Moreover, it contributes to the survival of the virus in the body through affecting the production of interferon. F protein also might play roles in the disease development, resulting in HCC. The existence of F protein affects cellular pathways through upregulating p53, c‐myc, cyclin D1, and phosphorylating Rb. This review will summarize these effects on immune system and related mechanisms in cellular pathways.

Microbial Pathogenesis

Immunization of mice by the co-administration of codon-optimized HPV16 E7 and lL12 genes against ... more Immunization of mice by the co-administration of codon-optimized HPV16 E7 and lL12 genes against HPV16-associated cervical cancer

Rabies constantly kills 59,000 people annually, mostly in Asia and Africa. Rabies, which is respo... more Rabies constantly kills 59,000 people annually, mostly in Asia and Africa. Rabies, which is responsible for 99% of human rabies cases, is totally preventable by standard vaccinations. In 2015, a global call for action was made by the WHO, OIE, FAO, and GARC to join forces toward the elimination of dog-transmitted human rabies by the year 2030. All the tools and protocols to reach that target are readily available, and the feasibility of dog rabies elimination has been proven. Countries should drive the changes needed to engage into this global movement. Certainly, countries in the EMR require taking more critical steps to reach the rabies elimination target by 2030. The international awareness campaign of the WRD is an excellent occasion to assess challenges and opportunities toward rabies elimination.