Ashraf Shoeib - Academia.edu (original) (raw)
Papers by Ashraf Shoeib
Archives of Medical Science, 2011
Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests tha... more Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests that the microbial community in the human intestine may play an important role in the pathogenesis of obesity. The aim of this study was to assess the differences in the composition of the intestinal microbiota between obese and normal weight Egyptian children and adults. The study included 79 subjects among whom 51 were obese (23 children and 28 adults), and 28 were subjects of normal weight (17 children and 11 adults). Faecal samples were collected from all subjects. Total DNA was extracted from collected stool samples and submitted to conventional PCR for detection of Firmicutes and Bacteroidetes. All the studied group was subjected to clinical and anthropometric evaluation. Laboratory assessment of fasting glucose, high-sensitivity C-reactive protein (hsCRP) and lipid profile was performed. The proportions of the phyla Firmicutes and Bacteroidetes were statistically significantly increased in the obese group compared to the normal weight group (p < 0.001, p = 0.003 respectively). The study also found a statistically significant positive trend for higher hsCRP in subjects with positive Firmicutes (p = 0.004). However, no associations were found between positive Bacteroidetes and hsCRP. The results of this study indicate that obesity in Egyptian children and adults is associated with compositional changes in faecal microbiota with increase in the phyla Firmicutes and Bacteroidetes. This could be considered when developing strategies to control obesity and its associated diseases by modifying the gut microbiota.
The Journal of the Egyptian Public Health Association, 2009
Enteric viruses are important causative agents of human diseases. Among the enteric viruses, reov... more Enteric viruses are important causative agents of human diseases. Among the enteric viruses, reoviruses and enteroviruses are prevalent in various aquatic environments. This study was carried out to detect and compare the presence of reoviruses and enteroviruses by ICC-PCR in one wastewater and three drinking water treatment plants, studying the possibility of using reoviruses as indicator of viral water pollution and genotyping of the isolated strains. One hundred and forty four drinking water and 76 wastewater samples were collected for two years. Reoviruses and enteroviruses were detected in 12.5% (18/144) and 8.3% (12/144) of total collected drinking water samples. In the studied wastewater treatment plant (WWTP), reoviruses were detected in 26% (20/76) of total collected samples while enteroviruses were detected in 21% (16/76) of the total collected samples. Phylogenic analysis revealed that our sequences were closely related to reovirus type 1, Lang strain and Human poliovirus...
The Journal of the Egyptian Public Health Association, 2015
The WHO estimated the annual rotavirus-related mortality among children below 5 years old in Egyp... more The WHO estimated the annual rotavirus-related mortality among children below 5 years old in Egypt in 2004 to be 30 deaths per 100 000, or out of an estimated 2616 deaths, 3.9% were because of rotavirus infection. The aim of this article was to study the epidemiology and circulating genotypes of rotaviruses in Cairo from 2011 to 2012. A total of 197 stool samples were collected from 130 inpatient children at the Cairo University Children Hospital and 67 outpatient children at the Al-Saff Children Clinic. The collected stool samples were then screened for rotavirus using enzyme immunoassay, followed by a screening for G-type and P-type using RT-PCR, and confirmation by sequence analysis. Rotavirus was detected in 39.1% (77/197) of the children, with a higher rate in inpatients (43.9%, 57/130) than in outpatients (29.9%, 20/67). There was an increase of rotavirus infection in the winter season. The majority of rotavirus cases (85.7%) occurred during the first year of life. The predomi...
Objective. The deficiency of clotting factor IX leads to sever bleeding disorder known as haemoph... more Objective. The deficiency of clotting factor IX leads to sever bleeding disorder known as haemophilia B or chrismas disease. It is X-linked recessive gene. Patients are treated with repeated injections of human plasma FIX concentrate or recombinant FIX protein; in which put them in risk of virulent viral infections. Therefore, the Egyptian murine species could be used as expression hosts for human FIX cDNA. Method. FIX cDNAs were studied in human and some Egyptian strains of mice and rats. RNA was extracted from liver samples. Specific primers were designed and FIX cDNAs synthesized by RT-PCR. Results. Revealed that two fragments from human FIX cDNA (sizes: 476 bp and 423 bp); three fragments from rat FIX cDNA (sizes:476 bp, 408 bp and 384 bp); and two fragments from mouse FIX cDNA (sizes: 476 bp and 384 bp) have been amplified. PCR product of the largest fragment 476 bp of all species has been purified and sequenced from liver rat samples. The nucleotide sequences of prepared fragment 476 bp from human and mouse FIX cDNAs were similar to that found in Gene Bank under accession numbers of M35672 and M23109, respectively. While, there were no similarities found in the nucleotide sequences of rat's fragment size 476 bp with those reported of Gene Bank database. Neighbor-joining phylogenetic tree showed no similarities between nucleotide sequences (fragment size 476 bp) of human FIX cDNA and those found in same position of the studied Egyptian murine species FIX cDNAs. Conclusion. Due to these differences in nucleotide sequences of FIX cDNAs, the gene expression of human FIX cDNA differs than those of Egyptian murine species. We suggest that the Egyptian murine species could be used as suitable models for gene therapy approaches which probably used in the treatment of haemophila B in human patients.
Logistics Information Management, 2003
The paper examined the nature of information technology (IT) outsourcing decision making and deve... more The paper examined the nature of information technology (IT) outsourcing decision making and developed a theoretical framework consisting of five phases of decision making. The phases augmented those of Simon and consisted of intelligence, analysis and planning, strategy ...
Archives of Medical Science, 2011
Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests tha... more Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests that the microbial community in the human intestine may play an important role in the pathogenesis of obesity. The aim of this study was to assess the differences in the composition of the intestinal microbiota between obese and normal weight Egyptian children and adults. The study included 79 subjects among whom 51 were obese (23 children and 28 adults), and 28 were subjects of normal weight (17 children and 11 adults). Faecal samples were collected from all subjects. Total DNA was extracted from collected stool samples and submitted to conventional PCR for detection of Firmicutes and Bacteroidetes. All the studied group was subjected to clinical and anthropometric evaluation. Laboratory assessment of fasting glucose, high-sensitivity C-reactive protein (hsCRP) and lipid profile was performed. The proportions of the phyla Firmicutes and Bacteroidetes were statistically significantly increased in the obese group compared to the normal weight group (p < 0.001, p = 0.003 respectively). The study also found a statistically significant positive trend for higher hsCRP in subjects with positive Firmicutes (p = 0.004). However, no associations were found between positive Bacteroidetes and hsCRP. The results of this study indicate that obesity in Egyptian children and adults is associated with compositional changes in faecal microbiota with increase in the phyla Firmicutes and Bacteroidetes. This could be considered when developing strategies to control obesity and its associated diseases by modifying the gut microbiota.
Archives of Medical Science, 2011
Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests tha... more Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests that the microbial community in the human intestine may play an important role in the pathogenesis of obesity. The aim of this study was to assess the differences in the composition of the intestinal microbiota between obese and normal weight Egyptian children and adults. The study included 79 subjects among whom 51 were obese (23 children and 28 adults), and 28 were subjects of normal weight (17 children and 11 adults). Faecal samples were collected from all subjects. Total DNA was extracted from collected stool samples and submitted to conventional PCR for detection of Firmicutes and Bacteroidetes. All the studied group was subjected to clinical and anthropometric evaluation. Laboratory assessment of fasting glucose, high-sensitivity C-reactive protein (hsCRP) and lipid profile was performed. The proportions of the phyla Firmicutes and Bacteroidetes were statistically significantly increased in the obese group compared to the normal weight group (p < 0.001, p = 0.003 respectively). The study also found a statistically significant positive trend for higher hsCRP in subjects with positive Firmicutes (p = 0.004). However, no associations were found between positive Bacteroidetes and hsCRP. The results of this study indicate that obesity in Egyptian children and adults is associated with compositional changes in faecal microbiota with increase in the phyla Firmicutes and Bacteroidetes. This could be considered when developing strategies to control obesity and its associated diseases by modifying the gut microbiota.
The Journal of the Egyptian Public Health Association, 2009
Enteric viruses are important causative agents of human diseases. Among the enteric viruses, reov... more Enteric viruses are important causative agents of human diseases. Among the enteric viruses, reoviruses and enteroviruses are prevalent in various aquatic environments. This study was carried out to detect and compare the presence of reoviruses and enteroviruses by ICC-PCR in one wastewater and three drinking water treatment plants, studying the possibility of using reoviruses as indicator of viral water pollution and genotyping of the isolated strains. One hundred and forty four drinking water and 76 wastewater samples were collected for two years. Reoviruses and enteroviruses were detected in 12.5% (18/144) and 8.3% (12/144) of total collected drinking water samples. In the studied wastewater treatment plant (WWTP), reoviruses were detected in 26% (20/76) of total collected samples while enteroviruses were detected in 21% (16/76) of the total collected samples. Phylogenic analysis revealed that our sequences were closely related to reovirus type 1, Lang strain and Human poliovirus...
The Journal of the Egyptian Public Health Association, 2015
The WHO estimated the annual rotavirus-related mortality among children below 5 years old in Egyp... more The WHO estimated the annual rotavirus-related mortality among children below 5 years old in Egypt in 2004 to be 30 deaths per 100 000, or out of an estimated 2616 deaths, 3.9% were because of rotavirus infection. The aim of this article was to study the epidemiology and circulating genotypes of rotaviruses in Cairo from 2011 to 2012. A total of 197 stool samples were collected from 130 inpatient children at the Cairo University Children Hospital and 67 outpatient children at the Al-Saff Children Clinic. The collected stool samples were then screened for rotavirus using enzyme immunoassay, followed by a screening for G-type and P-type using RT-PCR, and confirmation by sequence analysis. Rotavirus was detected in 39.1% (77/197) of the children, with a higher rate in inpatients (43.9%, 57/130) than in outpatients (29.9%, 20/67). There was an increase of rotavirus infection in the winter season. The majority of rotavirus cases (85.7%) occurred during the first year of life. The predomi...
Objective. The deficiency of clotting factor IX leads to sever bleeding disorder known as haemoph... more Objective. The deficiency of clotting factor IX leads to sever bleeding disorder known as haemophilia B or chrismas disease. It is X-linked recessive gene. Patients are treated with repeated injections of human plasma FIX concentrate or recombinant FIX protein; in which put them in risk of virulent viral infections. Therefore, the Egyptian murine species could be used as expression hosts for human FIX cDNA. Method. FIX cDNAs were studied in human and some Egyptian strains of mice and rats. RNA was extracted from liver samples. Specific primers were designed and FIX cDNAs synthesized by RT-PCR. Results. Revealed that two fragments from human FIX cDNA (sizes: 476 bp and 423 bp); three fragments from rat FIX cDNA (sizes:476 bp, 408 bp and 384 bp); and two fragments from mouse FIX cDNA (sizes: 476 bp and 384 bp) have been amplified. PCR product of the largest fragment 476 bp of all species has been purified and sequenced from liver rat samples. The nucleotide sequences of prepared fragment 476 bp from human and mouse FIX cDNAs were similar to that found in Gene Bank under accession numbers of M35672 and M23109, respectively. While, there were no similarities found in the nucleotide sequences of rat's fragment size 476 bp with those reported of Gene Bank database. Neighbor-joining phylogenetic tree showed no similarities between nucleotide sequences (fragment size 476 bp) of human FIX cDNA and those found in same position of the studied Egyptian murine species FIX cDNAs. Conclusion. Due to these differences in nucleotide sequences of FIX cDNAs, the gene expression of human FIX cDNA differs than those of Egyptian murine species. We suggest that the Egyptian murine species could be used as suitable models for gene therapy approaches which probably used in the treatment of haemophila B in human patients.
Logistics Information Management, 2003
The paper examined the nature of information technology (IT) outsourcing decision making and deve... more The paper examined the nature of information technology (IT) outsourcing decision making and developed a theoretical framework consisting of five phases of decision making. The phases augmented those of Simon and consisted of intelligence, analysis and planning, strategy ...
Archives of Medical Science, 2011
Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests tha... more Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests that the microbial community in the human intestine may play an important role in the pathogenesis of obesity. The aim of this study was to assess the differences in the composition of the intestinal microbiota between obese and normal weight Egyptian children and adults. The study included 79 subjects among whom 51 were obese (23 children and 28 adults), and 28 were subjects of normal weight (17 children and 11 adults). Faecal samples were collected from all subjects. Total DNA was extracted from collected stool samples and submitted to conventional PCR for detection of Firmicutes and Bacteroidetes. All the studied group was subjected to clinical and anthropometric evaluation. Laboratory assessment of fasting glucose, high-sensitivity C-reactive protein (hsCRP) and lipid profile was performed. The proportions of the phyla Firmicutes and Bacteroidetes were statistically significantly increased in the obese group compared to the normal weight group (p < 0.001, p = 0.003 respectively). The study also found a statistically significant positive trend for higher hsCRP in subjects with positive Firmicutes (p = 0.004). However, no associations were found between positive Bacteroidetes and hsCRP. The results of this study indicate that obesity in Egyptian children and adults is associated with compositional changes in faecal microbiota with increase in the phyla Firmicutes and Bacteroidetes. This could be considered when developing strategies to control obesity and its associated diseases by modifying the gut microbiota.