Asmaa Metwally - Academia.edu (original) (raw)

Papers by Asmaa Metwally

Research paper thumbnail of Studies on Nematode Parasites Infecting Freshwater Fish in Qena Governorate

Kafrelsheikh Veterinary Medical Journal, 2015

The present study was carried out to make a spot light on nematode parasites infecting Freshwater... more The present study was carried out to make a spot light on nematode parasites infecting Freshwater fish at Qena Governorate; including prevalence, seasonal dynamics of infection; intensity and morphology of the recovered parasite species. It was found that the infection rate of different nematode among freshwater fish in Qena Governorate was (13%). Lates niloticus has highest infection rate (27.8%) followed by Carias gariepinus (8.3%) the Oreochromis niloticus (2.3%). Concerning the seasonal prevalence Winter has highest infection rate followed by Autumn then Summer and Spring. Four nematodes were recovered and identified as Paracamallanus cyathopharynx (2.8%) in Clarias lazera; Procamallanus laevionchus (2.8%) in Clarias lazera; Contracaecum sp. (2.8%) in Oreochromis niloticus and Clarias lazera and (26.4%) in Lates niloticus and Dichelyne niloticus (4.2%) in Lates niloticus. The mean intensity of infection of Contracaecum sp. among Oreochromis niloticus., Clarias gariepinus and Lates niloticus was 2%, 22% and 20% respectively.

Research paper thumbnail of Detection of the intranuclear microsporidian Enterospora nucleophila in gilthead sea bream by in situ hybridization

Journal of Fish Diseases, 2019

Enterospora nucleophila is an intranuclear microsporidian responsible for emaciative microsporidi... more Enterospora nucleophila is an intranuclear microsporidian responsible for emaciative microsporidiosis of gilthead sea bream (GSB). Its minute size and cryptic nature make it easily misdiagnosed. An in situ hybridization (ISH) technique based on antisense oligonucleotide probes specific for the parasite was developed and used in clinically infected GSB in combination with calcofluor white stain (CW) and other histopathological techniques. The ISH method was found to label very conspicuously the cells containing parasite stages, with the signal concentrating in merogonial and sporogonial plasmodia within the infected cell nuclei. Comparison with CW demonstrated limited ISH signal in cells containing mature spores, which was attributed mostly to the scarcity of probe targets present in these stages. Although spores were detected in other organs of the digestive system as well as in the peripheral blood, proliferative stages or parasite reservoirs were not found in this work outside the...

Research paper thumbnail of Molecular and Phylogenetic Status of Fasciola sp., of Cattle in Qena, Upper Egypt

Pakistan Journal of Biological Sciences, 2013

Research paper thumbnail of Microscopical and serological studies on Sarcocystis infection with first report of S. cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt

Journal of Parasitic Diseases, 2013

This study was performed for the purpose of investigating the prevalence and the species composit... more This study was performed for the purpose of investigating the prevalence and the species composition of Sarcocystis spp. in buffaloes in Assiut province, Egypt. Macroscopically we reported the infection of buffaloes with Sarcocystis fusiformis, while microscopically three Sarcocystis species (Sarcocystis cruzi, Sarcocystis levinei and Sarcocystis hominis) cysts were recognized, and were differentiated by their morphological features using both histopathological sections and electron microscope scanning. Regarding the prevalence of Sarcocystis species among buffaloes in Assiut province, we reported that, using gross examination of 90 buffaloes' esophagus, only 23 samples out of 90 (25.5 %) were found to be infected; on the other hand, by using microscopical examination, the prevalence was 27.7 % (25 samples out of 90 samples were found to be infected). Using ELISA, 85 samples out of 90 (94.4 %) were found positive, an overall prevalence of 94.4 %. In this work we concluded that customary meat inspection methods in abattoirs in Egypt are insufficient for detecting Sarcocystis infection. Due to the presence of hidden or microscopic cysts, we strongly recommend the use of combined microscopical examination and ELISA for Sarcocystis diagnosis, to avoid human infection of such zoonotic parasite and to control the consequent disease. In addition, this study introduced the first report of S. cruzi in buffaloes in Egypt, and proved the hypothesis that S. cruzi is able to use animals such as water buffalo as intermediate hosts.

Research paper thumbnail of A Survey on Ectoparasites of Some Freshwater Fish at Qena Governorate, Upper Egypt

Kafrelsheikh Veterinary Medical Journal, 2013

Three species of fish (Oreochromis niloticus, Clarias lazera and Lates niloticus) were collected ... more Three species of fish (Oreochromis niloticus, Clarias lazera and Lates niloticus) were collected from Qena, Upper Egypt during the period from April, 2012 to the end of March, 2013. Upon their inspection, of 6 of ectoparasites species were recovered 3 monogeneans (Cichlidogyrus arthracanthus, Euryhaliotrema lovejoyi and Dactylogyrus spp.), two crustaceans (Lamproglena monody and Ergasilus sarsi) and one protozoan (Henneguya assuiti). The overall prevalence of infection was 12.03% and the highest peak was observed in O. niloticus with 19.4% (14/72) and the lowest percent was recorded in L. niloticus with 5.6% (4/72). Also, seasonal dynamics among the examined fish were recorded. The study revealed the occurrence of one monogenean trematode; Euryhaliotrema lovejoyi for the first time in Upper Egypt.

Research paper thumbnail of Studies on Nematode Parasites Infecting Freshwater Fish in Qena Governorate

Kafrelsheikh Veterinary Medical Journal, 2015

The present study was carried out to make a spot light on nematode parasites infecting Freshwater... more The present study was carried out to make a spot light on nematode parasites infecting Freshwater fish at Qena Governorate; including prevalence, seasonal dynamics of infection; intensity and morphology of the recovered parasite species. It was found that the infection rate of different nematode among freshwater fish in Qena Governorate was (13%). Lates niloticus has highest infection rate (27.8%) followed by Carias gariepinus (8.3%) the Oreochromis niloticus (2.3%). Concerning the seasonal prevalence Winter has highest infection rate followed by Autumn then Summer and Spring. Four nematodes were recovered and identified as Paracamallanus cyathopharynx (2.8%) in Clarias lazera; Procamallanus laevionchus (2.8%) in Clarias lazera; Contracaecum sp. (2.8%) in Oreochromis niloticus and Clarias lazera and (26.4%) in Lates niloticus and Dichelyne niloticus (4.2%) in Lates niloticus. The mean intensity of infection of Contracaecum sp. among Oreochromis niloticus., Clarias gariepinus and Lates niloticus was 2%, 22% and 20% respectively.

Research paper thumbnail of Detection of the intranuclear microsporidian Enterospora nucleophila in gilthead sea bream by in situ hybridization

Journal of Fish Diseases, 2019

Enterospora nucleophila is an intranuclear microsporidian responsible for emaciative microsporidi... more Enterospora nucleophila is an intranuclear microsporidian responsible for emaciative microsporidiosis of gilthead sea bream (GSB). Its minute size and cryptic nature make it easily misdiagnosed. An in situ hybridization (ISH) technique based on antisense oligonucleotide probes specific for the parasite was developed and used in clinically infected GSB in combination with calcofluor white stain (CW) and other histopathological techniques. The ISH method was found to label very conspicuously the cells containing parasite stages, with the signal concentrating in merogonial and sporogonial plasmodia within the infected cell nuclei. Comparison with CW demonstrated limited ISH signal in cells containing mature spores, which was attributed mostly to the scarcity of probe targets present in these stages. Although spores were detected in other organs of the digestive system as well as in the peripheral blood, proliferative stages or parasite reservoirs were not found in this work outside the...

Research paper thumbnail of Molecular and Phylogenetic Status of Fasciola sp., of Cattle in Qena, Upper Egypt

Pakistan Journal of Biological Sciences, 2013

Research paper thumbnail of Microscopical and serological studies on Sarcocystis infection with first report of S. cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt

Journal of Parasitic Diseases, 2013

This study was performed for the purpose of investigating the prevalence and the species composit... more This study was performed for the purpose of investigating the prevalence and the species composition of Sarcocystis spp. in buffaloes in Assiut province, Egypt. Macroscopically we reported the infection of buffaloes with Sarcocystis fusiformis, while microscopically three Sarcocystis species (Sarcocystis cruzi, Sarcocystis levinei and Sarcocystis hominis) cysts were recognized, and were differentiated by their morphological features using both histopathological sections and electron microscope scanning. Regarding the prevalence of Sarcocystis species among buffaloes in Assiut province, we reported that, using gross examination of 90 buffaloes' esophagus, only 23 samples out of 90 (25.5 %) were found to be infected; on the other hand, by using microscopical examination, the prevalence was 27.7 % (25 samples out of 90 samples were found to be infected). Using ELISA, 85 samples out of 90 (94.4 %) were found positive, an overall prevalence of 94.4 %. In this work we concluded that customary meat inspection methods in abattoirs in Egypt are insufficient for detecting Sarcocystis infection. Due to the presence of hidden or microscopic cysts, we strongly recommend the use of combined microscopical examination and ELISA for Sarcocystis diagnosis, to avoid human infection of such zoonotic parasite and to control the consequent disease. In addition, this study introduced the first report of S. cruzi in buffaloes in Egypt, and proved the hypothesis that S. cruzi is able to use animals such as water buffalo as intermediate hosts.

Research paper thumbnail of A Survey on Ectoparasites of Some Freshwater Fish at Qena Governorate, Upper Egypt

Kafrelsheikh Veterinary Medical Journal, 2013

Three species of fish (Oreochromis niloticus, Clarias lazera and Lates niloticus) were collected ... more Three species of fish (Oreochromis niloticus, Clarias lazera and Lates niloticus) were collected from Qena, Upper Egypt during the period from April, 2012 to the end of March, 2013. Upon their inspection, of 6 of ectoparasites species were recovered 3 monogeneans (Cichlidogyrus arthracanthus, Euryhaliotrema lovejoyi and Dactylogyrus spp.), two crustaceans (Lamproglena monody and Ergasilus sarsi) and one protozoan (Henneguya assuiti). The overall prevalence of infection was 12.03% and the highest peak was observed in O. niloticus with 19.4% (14/72) and the lowest percent was recorded in L. niloticus with 5.6% (4/72). Also, seasonal dynamics among the examined fish were recorded. The study revealed the occurrence of one monogenean trematode; Euryhaliotrema lovejoyi for the first time in Upper Egypt.