Asmoro Lelono - Academia.edu (original) (raw)

Papers by Asmoro Lelono

AIP conference proceedings, 2024

Bio web of conferences/BIO web of conferences, 2024

Determining the sex of the embryo during incubation is crucial for the poultry industry. The prim... more Determining the sex of the embryo during incubation is crucial for the poultry industry. The primary problem to comprehend in this study is the manipulation of the sex ratio and also the egg production. The study will concentrate on how two distinct methods of pairing parents-one male with one female and another male with three females-affect egg production, the average egg mass, and the sex offspring ratio. As the animal model, we employ the layer strain of the Japanese Quail (Coturnix japonica) since they produce continuous eggs each day and easy to handle. The Japanese quail pairs were housed for seven days prior to the start of egg collection. These eggs were nurtured for up to three weeks after hatching until the sexing could be determined. When compared to a pair of three females, the egg production of a single pair was much higher when alternative pattern schemes were used. However, the mass of their typical eggs is remarkably similar. The male to female chick offspring sex ratio regularly showed to be 1:1 or equal. This finding could serve as the foundation for further research into the impact of partner pairing on another avian layer strain.

Life Science and Biotechnology, Nov 6, 2023

Salmonella is a gram-negative bacteria commonly found in poultry-based products such as eggs and ... more Salmonella is a gram-negative bacteria commonly found in poultry-based products such as eggs and meat, which can affect quality and human health. The presence of these bacteria in poultry management can be traced to feed, water, substrate, and interactions with the environment. This study aims to investigate the immune system of the avian through injection of Salmonella typhimurium colonies at the age of the immune system begins to be produced independently. Salmonella culture at a density of 10-8 and 10-12 was injected into the digestive tract, and the increase in body temperature was measured immediately after injection. Two weeks later, an analysis of leukocyte differentiation was carried out. We found that Salmonella injection affected body temperature and the rate of body mass increased in all treatments and sex. This indicates that the immune system was activated even though the leukocyte differentiation indicators did not show any significant results. Chicks at the age of one month can show an immune response to bacterial infections through self-defense mechanisms. Further research needs to be carried out to understand whether leukocyte differentiation stimulates leukocyte variation with age.

Jurnal Inovasi Sains dan Teknologi untuk Masyarakat

Community participation-based waste management has been carried out by DKLH Jember Regency throug... more Community participation-based waste management has been carried out by DKLH Jember Regency through the establishment of the Main Waste Bank its derivative organization, namely the Unit Waste Bank at a lower level. In this system, the effort being made is the integration of waste management, starting from the household as a producer, followed by sorting, then depositing it to BSU and ending with delivery to a temporary warehouse so that the waste is handled properly. This article aims to explore the potentials and problems encountered in the management of a Waste Bank which involves the participation of residents while still paying attention to aspects of economic feasibility. The research was carried out using the analitcal descriptive via interview and analysis of primary data owned by BSU ASRI BMP with due observance of scientific principles and maintaining the independence and interests of the authors. From the results of the research it is known that waste valuation efforts by p...

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021), Dec 22, 2022

Berkala Sainstek (E-journal), Dec 6, 2021

The taste of sensitivity of phenylthiocarbamide (PTC), is autosomal dominant trait inherited whil... more The taste of sensitivity of phenylthiocarbamide (PTC), is autosomal dominant trait inherited while the colour blindness is a sex linked genetic trait on the X chromosome. The purpose of this study was to determine the distribution of taster and non taster phenotypes, prevalence of color blindness, frequency of taster and non taster alleles, and frequency of color blind alleles, as well as pedigree analysis in non taster and color blind families. The research was conducted on the Tengger tribe, in Ranupani village, Senduro, Lumajang. Determination of the sample is carryout randomly. Detection of the ability to taste PTC was respondents to taste the PTC solution from the lowest concentration of 0.32mg/L (P13) to the highest concentration of 1300 mg/L (P1). Color blindness detection by the Ishihara method. The results of the study showed that the distribution of the taster was 98.1% while the non-taster was 1.9%. The allele frequency of the dominant taster (T) was 0.86 and the recessive allele non taster (t) is 0.14. The prevalence of color blindness in the population of the Tengger tribe was 0.63% and the allele frequency for color blindness was 0.013. The pedigree analysis of non taster family showed that non taster individuals were born from taster couple (Tt) or from couple of non taster (tt) with tasters (Tt) heterozigot. While the pattern of inheritance of color blindness was criss-cross inheritance pattern, which is passed from mother to son. Kemampuan mengecap rasa pahit Phenylthiocarbamide (PTC) merupakan pewarisan autosomal sedangkan buta warna adalah kelainan genetik yang disebabkan oleh gen terangkai kromosom kelamin X. Tujuan dari penelitian ini adalah untuk mengetahui distribusi fenotip taster, non taster, prevalensi buta warna serta frekuensi alel baik taster, non taster dan buta warna. Selain itu juga melakukan analisis pedigree terhadap keluarga non taster dan buta warna. Pengambilan sampel dilakukan pada suku Tengger, di desa Ranupani, kecamatan Senduro, kabupaten Lumajang. Penentuan sampel dilakukan secara acak. Deteksi kemampuan mengecap PTC dilakukan dengan cara responden diminta merasakan larutan PTC. Larutan PTC yang digunakan adalah dari konsentrasi terendah 0,32mg/L (P13) hingga konsentrasi tertinggi 1300 mg/L (P1). Deteksi kelainan buta warna dilakukan dengan metode Ishihara. Hasil penelitian terhadap kemampuan mengecap PTC pada suku Tengger menunjukan distribusi fenotip taster sebesar 98,1 % sedangkan non taster sebesar 1,9%. Nilai frekuensi alel dominan taster (T) sebesar 0,86 dan alel resesif (t) non taster sebesar 0,14. Prevalensi buta warna pada populasi suku Tengger di Desa Ranupani adalah sebesar 0,63% dan frekuensi alel buta warna sebesar 0,013. Hasil analisis pedigree menunjukkan bahwa individu non taster yang ditemukan pada populasi suku Tengger dilahirkan dari pasangan taster heterozigot (Tt) atau dari hasil perkawinan non taster (tt) dengan taster heterozigotik (Tt). Sedangkan pola pewarisan buta warna menunjukkan pola pewarisan bersilang yaitu diwariskan dari ibu ke anak laki-laki.

The exposure of yolk androgens can positively stimulate chick growth and competitive ability but ... more The exposure of yolk androgens can positively stimulate chick growth and competitive ability but may negatively affect immunity. It has been hypothesized that only chicks from immunologically superior fathers can bear the cost of prenatal exposure to high androgen levels. To test this hypothesis we paired roosters from two selection lines, one up- and one down-selected for natural antibodies, with hens from a control line. We measured yolk testosterone and androstenedione levels, and we injected the treatment group of eggs of each female with testosterone suspended in sesame oil and the control group with sesame oil only. We then measured hatching success, growth, and characterised the humoral and cellular immune responses using three different challenges: a PHA, an LPS, and an SRBC challenge. We found that the hatching success, body mass, initial levels of natural antibodies, and the chicks immunological responses to the three different challenges development were affected neither ...

The research has been held in the aim to know the Pattern prediction of Aedes aegypti as main vec... more The research has been held in the aim to know the Pattern prediction of Aedes aegypti as main vector of DHF in Jember, East Java Indonesia. It covered of the number of incidence and cluster of case in the peak of DHF epidemic in rainy season. The data were collected from November 1st 2005 until February 28th 2006 and November 1st until February 28th 2007. The results show that between two peak season there were 433 cases of DHF in 2005-2006 and 66 case in 2006-2007. Based on the prediction of vector distribution there were six large and four small groups in the year of 2005-2006 compared with one large and one small group in the year of 2006-2007. Most of the vector habitat close related with human settlement and the climate perhaps plays the main role in outbreak of Ae. aegypti population. The lows incidence in the year of 2006-2007 perhaps because of the implementation of control methods drive by official government of Jember Residence call “jum’at bersih” or clean Friday with par...

Life-history traits vary largely across species and several physiological parameters have been pr... more Life-history traits vary largely across species and several physiological parameters have been proposed to be associated with life-history variation, such as metabolic rates, glucocorticoids, and oxidative stress. Interestingly, the association between thyroid hormones (THs) and life history variation has never been considered, despite a close interaction between THs and these physiological traits. Because of the crucial effects on embryonic development, THs can also induce transgenerational plasticity when transferred to developing offspring, for instance, via egg yolks in birds. In this study, we compiled a unique data set of maternal yolk THs in 34 bird species across 17 families and 6 orders, and tested for associations with various life-history traits. Our phylogenetic mixed models indicated that both concentrations and total amounts of the two most important forms of THs (T3 and T4) were higher in the eggs of migratory species than in those of resident species, and that there ...

BIOEDUKASI

Salak seed are used as natural diuretics, it's contain secondary metabolites i.e alkaloids, f... more Salak seed are used as natural diuretics, it's contain secondary metabolites i.e alkaloids, flavonoids and tannins. Diuretics are compounds that can increase the rate of excretion of urine volume and increase the excretion of mineral salts in the urine. The study was to determine the diuretic effect of salak seed extract on the histopathological structure of the kidney. Salak seed extract was made by maceration method using 70% ethanol and then evaporated. Rats were divided into 4 treatment groups, negative control and 3 treatment groups with the administration of salak seed extract for 7 days orally at a dose of 0.28g/kg body weight, 0.56g/kg body weight and 1.12g/kg body weight. The results showed that the treatment of salak seed extract orally for 7 days could increase urine volume. The treatment of dose 1.12g/kg body weight had highest diuretic effect as indicated by the volume of urine produced 5.00 ml. The treatment salak seed extract had no effect on creatinine and u...

Scientific Reports

hatched. Both the percentage showing development as well as the percentage hatched did not differ... more hatched. Both the percentage showing development as well as the percentage hatched did not differ between the groups (proportion test, egg development: Z = 1.453, p = 0.147; hatching: Z = 0.852, p = 0.397).

The relative effects of a diffuse disturbance (alteration of land use from forest to plantation) ... more The relative effects of a diffuse disturbance (alteration of land use from forest to plantation) and a point-source disturbance (a village and its associated coffee-processing plant, within the plantation) on longitudinal variation in water chemistry and macroinvertebrate community composition were assessed in Kali Dinoyo, a small upland stream in East Java, Indonesia. Four sites were sampled. The catchments of the two most-upstream sites were covered primarily by rainforest, while the two lower sites fell within a coffee plantation. The lowest site was downstream of a small village and its associated coffee-processing plant. Most of the variance in all water quality variables and in several community composition measures was explained by the difference between plantation and forest sites. Comparatively small differences in total suspended solids and macroinvertebrate community composition were observed downstream of the village. Diffuse disturbances associated with land clearance and plantation agriculture therefore appear to have a larger impact on the ecology of Kali Dinoyo than the point source impacts associated with the village. More robust and powerful study designs to formally test these findings are discussed.

Animals' life-history traits vary largely along many continuums across species and several ph... more Animals' life-history traits vary largely along many continuums across species and several physiological parameters have been proposed as possible mediators that drive the life-history variation, such as metabolic rates, glucocorticoids, and oxidative stress. Interestingly, thyroid hormones (THs), despite closely interacting with these physiological parameters, have never been considered as such mediators. Because of the crucial effects on embryonic development, THs can also induce transgenerational plasticity when transferred into developing offspring, for instance, via egg yolks in birds. In this study, we compiled a unique data set of maternal yolk THs in 34 bird species across 17 families and 6 orders and analysed them with data of life-history traits along several life-history continuums. Our phylogenetic mixed models estimated higher deposition of the two most important forms of THs - T3 and T4 - in both concentrations and total amounts in migratory species than resident s...

Biology Open

Exposure of yolk androgens can positively stimulate chick growth and competitive ability, but may... more Exposure of yolk androgens can positively stimulate chick growth and competitive ability, but may negatively affect immunity. It has been hypothesized that only chicks from immunologically superior fathers can bear the cost of prenatal exposure to high androgen levels. To test this hypothesis, we paired roosters from two selection lines, one up-and one down-selected for natural antibodies (NAbs), with hens from a control line. We measured yolk testosterone and androstenedione levels, and we injected the treatment group of eggs of each female with testosterone suspended in sesame oil and the control group with sesame oil only. We then measured hatching success and growth, and characterized the humoral and cellular immune responses using three different challenges: a phyto-hemagglutinin, a lipopolysaccharide and a sheep red blood cell challenge. We found that the hatching success, body mass, initial levels of natural antibodies and the chicks' immunological responses to the three different challenges and development were affected neither by paternal immunocompetence nor by treatment. These results do not support the hypothesis that chicks from low-NAb line fathers are more sensitive to testosterone exposure during embryonic development than chicks from high-NAb line fathers.