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The present study was conducted to determine the earliest stage of gestation at which pregnancy c... more The present study was conducted to determine the earliest stage of gestation at which pregnancy could be detected through transabdominal ultrasonography, to describe development characteristics of pregnancy from day 26 to day 60 of gestation, and to assess the accuracy of flock pregnancy diagnosis on day 75 after ram introduction in "Balkhi sheep" of Pakistan. A real time B-mode ultrasound scanner equipped with a 3.5 MHz probe was used for this purpose. A 100% accuracy of the pregnancy diagnosis was achieved at day 42 of gestation. Placentomes and leg buds were visible in 100% cases between 45 and 50 days of gestation, and vertebral column was apparent in 100% ewes between 51 and 55 days of gestation. Accuracy of flock pregnancy diagnosis was assessed by once only ultrasono-graphy at 75 th day after introduction of ram in 71 ewes over two breeding seasons. Flock pregnancy test performed at this day was 97% accurate.
British Medical Journal, 2004
European Journal of Histochemistry, 2010
The objective of this study was to determine the distribution of somatotrophs and lactotrophs and... more The objective of this study was to determine the distribution of somatotrophs and lactotrophs and conduct a morphometrical analysis of immunoreactive somatotrophs and lactotrophs in the pituitary glands of White Leghorn Hens (Gallus domesticus) during the period of induced moult. We divided the periods of induced moulting into three phases viz. 7, 14 and 21 days. The labeled alkalinephsphatase method with anti-GH (growth hormone) and anti-PRL (prolactin) as a primary antibody was used to detect somatotrophs and lactotrophs, in the midsagital sections of chicken adenohypophysis. Immunohistochemistry showed that somatotrophs are not only confined to the cephalo-caudal axis but can also be found in the caudal lobe; while lactotrophs were distributed in both lobes of the anterior pituitary gland at all stages of moulting (7, 14 and 21 days). Lactotrophs were of different shapes but somatotrophs were oval to round in morphology. At the given stages of induced moulting, some hypertrophied lactotrophs were also present after 7 days of induced moult in the anterior pituitary gland. However, there were moulting-related changes: from 7 to 21 days of induced moulting the immunoreactive-PRL cell population decreased, while the mean lactotroph size was more than that of somatotrophs. Basic quantitative and morphological information relating to somatotrophs and lactotrophs during the period of induced moult in laying hens is reported here and the changes brought about by induced moulting are restricted to PRL positive cells rather than GH positive cells. This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0).
The present study was conducted to determine the earliest stage of gestation at which pregnancy c... more The present study was conducted to determine the earliest stage of gestation at which pregnancy could be detected through transabdominal ultrasonography, to describe development characteristics of pregnancy from day 26 to day 60 of gestation, and to assess the accuracy of flock pregnancy diagnosis on day 75 after ram introduction in "Balkhi sheep" of Pakistan. A real time B-mode ultrasound scanner equipped with a 3.5 MHz probe was used for this purpose. A 100% accuracy of the pregnancy diagnosis was achieved at day 42 of gestation. Placentomes and leg buds were visible in 100% cases between 45 and 50 days of gestation, and vertebral column was apparent in 100% ewes between 51 and 55 days of gestation. Accuracy of flock pregnancy diagnosis was assessed by once only ultrasono-graphy at 75 th day after introduction of ram in 71 ewes over two breeding seasons. Flock pregnancy test performed at this day was 97% accurate.
British Medical Journal, 2004
European Journal of Histochemistry, 2010
The objective of this study was to determine the distribution of somatotrophs and lactotrophs and... more The objective of this study was to determine the distribution of somatotrophs and lactotrophs and conduct a morphometrical analysis of immunoreactive somatotrophs and lactotrophs in the pituitary glands of White Leghorn Hens (Gallus domesticus) during the period of induced moult. We divided the periods of induced moulting into three phases viz. 7, 14 and 21 days. The labeled alkalinephsphatase method with anti-GH (growth hormone) and anti-PRL (prolactin) as a primary antibody was used to detect somatotrophs and lactotrophs, in the midsagital sections of chicken adenohypophysis. Immunohistochemistry showed that somatotrophs are not only confined to the cephalo-caudal axis but can also be found in the caudal lobe; while lactotrophs were distributed in both lobes of the anterior pituitary gland at all stages of moulting (7, 14 and 21 days). Lactotrophs were of different shapes but somatotrophs were oval to round in morphology. At the given stages of induced moulting, some hypertrophied lactotrophs were also present after 7 days of induced moult in the anterior pituitary gland. However, there were moulting-related changes: from 7 to 21 days of induced moulting the immunoreactive-PRL cell population decreased, while the mean lactotroph size was more than that of somatotrophs. Basic quantitative and morphological information relating to somatotrophs and lactotrophs during the period of induced moult in laying hens is reported here and the changes brought about by induced moulting are restricted to PRL positive cells rather than GH positive cells. This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0).