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ABSTRACTLymph nodes are the primary site of replication for measles virus (MeV). Here, we modeled... more ABSTRACTLymph nodes are the primary site of replication for measles virus (MeV). Here, we modeled MeV infection in human tonsil explants, utilizing a clinical strain of MeV that expresses GFP. We show that MeV replicates efficiently in this tissue as measured by increasing infectious virus production and GFP+ cells (>10% of tonsillar cells by day 8). Using scRNA-Seq, we identified 29 cell populations, all of which were susceptible to MeV. While T cells were the most abundant cell type in the lymphoid explants, B cells were the dominant infected population. Flow cytometry analysis revealed that the preferential infection of B cells was associated with higher CD150 expression. We found that while germinal center B cells were the largest population of infected B cells, there were no differences in susceptibility to MeV among individual B cell subsets. Among CD3+ T cells, infection in both the CD4+ and CD8+ compartments displayed bias towards antigen experienced subsets and away from...
Journal of Luminescence, 2019
Ce:YAG/YAG nanophosphors are synthesized using a two-step seed mediated glycothermal process. The... more Ce:YAG/YAG nanophosphors are synthesized using a two-step seed mediated glycothermal process. The need for the proposed process arises from the high sensitivity of Ce 3+ to its local environment, the highly defective surfaces of nanoparticles, and increased proportion of Ce atoms near the surface due to the high ratio of surface area to volume of nanoparticles. Shell thickness was controlled by varying the molar concentration of material added during the second step of synthesis relative to the starting amount, denoted as the shell-to-seed ratio (SSR). It was found that an SSR of 1:1 yielded an average particle size of 186 ± 59 nm with a marked enhancement in photoluminescence and photostability compared to particles with no shell. EELS linescans confirm localization of the dopant to the core of the crystal.
ABSTRACTLymph nodes are the primary site of replication for measles virus (MeV). Here, we modeled... more ABSTRACTLymph nodes are the primary site of replication for measles virus (MeV). Here, we modeled MeV infection in human tonsil explants, utilizing a clinical strain of MeV that expresses GFP. We show that MeV replicates efficiently in this tissue as measured by increasing infectious virus production and GFP+ cells (>10% of tonsillar cells by day 8). Using scRNA-Seq, we identified 29 cell populations, all of which were susceptible to MeV. While T cells were the most abundant cell type in the lymphoid explants, B cells were the dominant infected population. Flow cytometry analysis revealed that the preferential infection of B cells was associated with higher CD150 expression. We found that while germinal center B cells were the largest population of infected B cells, there were no differences in susceptibility to MeV among individual B cell subsets. Among CD3+ T cells, infection in both the CD4+ and CD8+ compartments displayed bias towards antigen experienced subsets and away from...
Journal of Luminescence, 2019
Ce:YAG/YAG nanophosphors are synthesized using a two-step seed mediated glycothermal process. The... more Ce:YAG/YAG nanophosphors are synthesized using a two-step seed mediated glycothermal process. The need for the proposed process arises from the high sensitivity of Ce 3+ to its local environment, the highly defective surfaces of nanoparticles, and increased proportion of Ce atoms near the surface due to the high ratio of surface area to volume of nanoparticles. Shell thickness was controlled by varying the molar concentration of material added during the second step of synthesis relative to the starting amount, denoted as the shell-to-seed ratio (SSR). It was found that an SSR of 1:1 yielded an average particle size of 186 ± 59 nm with a marked enhancement in photoluminescence and photostability compared to particles with no shell. EELS linescans confirm localization of the dopant to the core of the crystal.