Austin Armstrong - Academia.edu (original) (raw)
Uploads
Papers by Austin Armstrong
Obstetrics and gynecology (New York. 1953. Online)/Obstetrics and gynecology, May 1, 2024
Journal of Psychiatric Practice, Mar 1, 2023
Journal of Clinical Psychopharmacology, Mar 1, 2023
Kansas journal of medicine, Mar 15, 2022
The primary care companion for CNS disorders, Sep 23, 2021
Reproduction, Jun 1, 2018
Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ... more Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ovarian follicles and accelerate reproductive aging. We characterized a mouse model of low-dose TBI to investigate how radiation affects the follicular and stromal compartments of the ovary. A single TBI dose of either 0.1 Gy or 1 Gy (Cesium-137 γ) was delivered to reproductively adult CD1 female mice, and sham-treated mice served as controls. Mice were euthanized either 2 weeks or 5 weeks post exposure, and ovarian tissue was harvested. To assess the ovarian reserve, we classified and counted the number of morphologically normal follicles in ovarian histologic sections for all experimental cohorts using an objective method based on immunohistochemistry for an oocytespecific protein (MSY2). 0.1 Gy did not affect that total number of ovarian follicles, whereas 1 Gy resulted in a dramatic loss. At two weeks, there was a significant reduction in all preantral follicles, but early antral and antral follicles were still present. By five weeks, there was complete depletion of all follicle classes. We examined stromal quality using histologic stains to visualize ovarian architecture and fibrosis and by immunohistochemistry and quantitative microscopy to assess cell proliferation, cell death and vasculature. There were no differences in the ovarian stroma across cohorts with respect to these markers, indicating that this compartment is more radio-resistant relative to the germ cells. These findings have implications for reproductive health and the field of fertility preservation because the radiation doses we examined mimic scatter doses experienced in typical therapeutic regimens.
Future Oncology, Feb 1, 2018
To characterize the clinical indications of females (<15 years old) undergoing ovarian tissue cry... more To characterize the clinical indications of females (<15 years old) undergoing ovarian tissue cryopreservation (OTC) through the Oncofertility Consortium's National Physicians Cooperative (OC-NPC). Patients & methods: The clinical indications of 114 females who underwent OTC were classified, and their incidence was compared with childhood cancer databases. Results: Leukemias/myeloproliferative diseases/myelodysplastic diseases and hemoglobinopathies were the most prevalent oncologic and nononcologic indications for OTC, respectively. The frequencies of malignant bone tumors and soft tissue and other extraosseous sarcomas were higher in the OC-NPC cohort relative to the general population, while CNS/intracranial/intraspinal neoplasms, retinoblastoma and hepatic tumors were lower. Conclusion: Those opting for OTC through the OC-NPC are at highest fertility risk, indicating that the appropriate patient populations are being identified.
Journal of Assisted Reproduction and Genetics, Jun 22, 2023
Journal of Assisted Reproduction and Genetics
Journal of Clinical Psychopharmacology
Journal of Psychiatric Practice
Kansas Journal of Medicine, 2022
The Primary Care Companion For CNS Disorders
Future Oncology
Aim: To characterize the clinical indications of females (<15 years old) undergoing ovarian ti... more Aim: To characterize the clinical indications of females (<15 years old) undergoing ovarian tissue cryopreservation (OTC) through the Oncofertility Consortium's National Physicians Cooperative (OC-NPC). Patients & methods: The clinical indications of 114 females who underwent OTC were classified, and their incidence was compared with childhood cancer databases. Results: Leukemias/myeloproliferative diseases/myelodysplastic diseases and hemoglobinopathies were the most prevalent oncologic and nononcologic indications for OTC, respectively. The frequencies of malignant bone tumors and soft tissue and other extraosseous sarcomas were higher in the OC-NPC cohort relative to the general population, while CNS/intracranial/intraspinal neoplasms, retinoblastoma and hepatic tumors were lower. Conclusion: Those opting for OTC through the OC-NPC are at highest fertility risk, indicating that the appropriate patient populations are being identified. [Formula: see text]
Reproduction (Cambridge, England), Jun 10, 2018
Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ... more Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ovarian follicles and accelerate reproductive aging. We characterized a mouse model of low dose TBI to investigate how radiation affects the follicular and stromal compartments of the ovary. A single TBI dose of either 0.1 Gy or 1 Gy (Cesium-137γ was delivered to reproductively adult CD1 female mice, and sham-treated mice served as controls. Mice were sacrificed either 2 weeks or 5 weeks post-exposure, and ovarian tissue was harvested. To assess the ovarian reserve, we classified and counted the number of morphologically normal follicles in ovarian histologic sections for all experimental cohorts using an objective method based on immunohistochemistry for an oocyte-specific protein (MSY2). 0.1 Gy did not affect that total number of ovarian follicles, whereas 1 Gy resulted in a dramatic loss. At two weeks, there was a significant reduction in all preantral follicles, but early antral and ...
Obstetrics and gynecology (New York. 1953. Online)/Obstetrics and gynecology, May 1, 2024
Journal of Psychiatric Practice, Mar 1, 2023
Journal of Clinical Psychopharmacology, Mar 1, 2023
Kansas journal of medicine, Mar 15, 2022
The primary care companion for CNS disorders, Sep 23, 2021
Reproduction, Jun 1, 2018
Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ... more Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ovarian follicles and accelerate reproductive aging. We characterized a mouse model of low-dose TBI to investigate how radiation affects the follicular and stromal compartments of the ovary. A single TBI dose of either 0.1 Gy or 1 Gy (Cesium-137 γ) was delivered to reproductively adult CD1 female mice, and sham-treated mice served as controls. Mice were euthanized either 2 weeks or 5 weeks post exposure, and ovarian tissue was harvested. To assess the ovarian reserve, we classified and counted the number of morphologically normal follicles in ovarian histologic sections for all experimental cohorts using an objective method based on immunohistochemistry for an oocytespecific protein (MSY2). 0.1 Gy did not affect that total number of ovarian follicles, whereas 1 Gy resulted in a dramatic loss. At two weeks, there was a significant reduction in all preantral follicles, but early antral and antral follicles were still present. By five weeks, there was complete depletion of all follicle classes. We examined stromal quality using histologic stains to visualize ovarian architecture and fibrosis and by immunohistochemistry and quantitative microscopy to assess cell proliferation, cell death and vasculature. There were no differences in the ovarian stroma across cohorts with respect to these markers, indicating that this compartment is more radio-resistant relative to the germ cells. These findings have implications for reproductive health and the field of fertility preservation because the radiation doses we examined mimic scatter doses experienced in typical therapeutic regimens.
Future Oncology, Feb 1, 2018
To characterize the clinical indications of females (<15 years old) undergoing ovarian tissue cry... more To characterize the clinical indications of females (<15 years old) undergoing ovarian tissue cryopreservation (OTC) through the Oncofertility Consortium's National Physicians Cooperative (OC-NPC). Patients & methods: The clinical indications of 114 females who underwent OTC were classified, and their incidence was compared with childhood cancer databases. Results: Leukemias/myeloproliferative diseases/myelodysplastic diseases and hemoglobinopathies were the most prevalent oncologic and nononcologic indications for OTC, respectively. The frequencies of malignant bone tumors and soft tissue and other extraosseous sarcomas were higher in the OC-NPC cohort relative to the general population, while CNS/intracranial/intraspinal neoplasms, retinoblastoma and hepatic tumors were lower. Conclusion: Those opting for OTC through the OC-NPC are at highest fertility risk, indicating that the appropriate patient populations are being identified.
Journal of Assisted Reproduction and Genetics, Jun 22, 2023
Journal of Assisted Reproduction and Genetics
Journal of Clinical Psychopharmacology
Journal of Psychiatric Practice
Kansas Journal of Medicine, 2022
The Primary Care Companion For CNS Disorders
Future Oncology
Aim: To characterize the clinical indications of females (<15 years old) undergoing ovarian ti... more Aim: To characterize the clinical indications of females (<15 years old) undergoing ovarian tissue cryopreservation (OTC) through the Oncofertility Consortium's National Physicians Cooperative (OC-NPC). Patients & methods: The clinical indications of 114 females who underwent OTC were classified, and their incidence was compared with childhood cancer databases. Results: Leukemias/myeloproliferative diseases/myelodysplastic diseases and hemoglobinopathies were the most prevalent oncologic and nononcologic indications for OTC, respectively. The frequencies of malignant bone tumors and soft tissue and other extraosseous sarcomas were higher in the OC-NPC cohort relative to the general population, while CNS/intracranial/intraspinal neoplasms, retinoblastoma and hepatic tumors were lower. Conclusion: Those opting for OTC through the OC-NPC are at highest fertility risk, indicating that the appropriate patient populations are being identified. [Formula: see text]
Reproduction (Cambridge, England), Jun 10, 2018
Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ... more Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ovarian follicles and accelerate reproductive aging. We characterized a mouse model of low dose TBI to investigate how radiation affects the follicular and stromal compartments of the ovary. A single TBI dose of either 0.1 Gy or 1 Gy (Cesium-137γ was delivered to reproductively adult CD1 female mice, and sham-treated mice served as controls. Mice were sacrificed either 2 weeks or 5 weeks post-exposure, and ovarian tissue was harvested. To assess the ovarian reserve, we classified and counted the number of morphologically normal follicles in ovarian histologic sections for all experimental cohorts using an objective method based on immunohistochemistry for an oocyte-specific protein (MSY2). 0.1 Gy did not affect that total number of ovarian follicles, whereas 1 Gy resulted in a dramatic loss. At two weeks, there was a significant reduction in all preantral follicles, but early antral and ...