Avinash Mishra - Academia.edu (original) (raw)

Uploads

Papers by Avinash Mishra

Research paper thumbnail of Isolation and characterization of extracellular polymeric substances from micro-algae Dunaliella salina under salt stress

Bioresource Technology - BIORESOURCE TECHNOL, 2009

Extracellular polymeric substances (EPSs), produced by Dunaliella salina strain, increase concomi... more Extracellular polymeric substances (EPSs), produced by Dunaliella salina strain, increase concomitantly with salt concentration and maximum (944mg/l) were obtained at 5M NaCl, whereas minimum (56mg/l) at 0.5M salinity. Emulsifying activity was measured in terms of strength to retain the emulsion and comparatively 85.76% retention was observed at 0.5M salinity thereafter it intends to decline. The FT-IR-spectra reveal characteristic functional groups NH stretching, asymmetrical CH stretching vibration of aliphatic CH2-group, CC stretching of aromatic, CN stretch of aliphatic amine, NH wag of primary amine and CX stretch of alkyl-halides with a stretching of COC, CO corresponding to the presence of carbohydrates. The FT-IR-spectra substantiated the presence of primary amine-group, aromatic-compound, halide-group, aliphatic alkyl-group and polysaccharides. Four monosaccharides (glucose, galactose, fructose and xylose) were also detected by HPLC analysis. Production of EPSs may allow fu...

Research paper thumbnail of Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum

Research paper thumbnail of Bioinformatics: New directions to know facts of life

Bioinformatics is a scientific discipline of discovering biological functions through analysis of... more Bioinformatics is a scientific discipline of discovering biological functions through analysis of molecular sequence information from DNA, RNA or proteins. It is the computer assisted data management discipline that enables one to understand life's processes precisely. It helps to discover better drug target after differentiating between healthy and diseased. Bioinformatics is being considered bedrock for the future of biotechnology and is being adopted worldwide by academicians, companies, national and international consortia. Statistical and computational analysis of the complex relationships between phenotypes and genotypes; animals and plants plays a crucial role in the future of biology.

Research paper thumbnail of Bioinformatics and Its Application in Agri-biotechnology

Research paper thumbnail of Rheological Properties of Grain Sorghum Dough As Determined By Mixography

Research paper thumbnail of Studies on extensigraphic properties and effect of salt addition in grain sorghum dough

Research paper thumbnail of Differential distribution and abundance of diazotrophic bacterial communities across different soil niches using a gene‐targeted clone library approach

Research paper thumbnail of Non-targeted metabolomics and scavenging activity of reactive oxygen species reveal the potential of Salicornia brachiata as a functional food

Research paper thumbnail of Purification and characterization of cellulase from a marine< i> Bacillus</i> sp. H1666: A potential agent for single step saccharification of seaweed biomass

Research paper thumbnail of Ectopic over-expression of peroxisomal ascorbate peroxidase (< i> SbpAPX</i>) gene confers salt stress tolerance in transgenic peanut (< i> Arachis hypogaea</i>)

Research paper thumbnail of Microbial Exopolysaccharides

Research paper thumbnail of Extracellular polymeric substances from two biofilm forming< i> Vibrio</i> species: Characterization and applications

Research paper thumbnail of Production and structural characterization of biosurfactant produced by an alkaliphilic bacterium,< i> Klebsiella</i> sp.: Evaluation of different carbon sources

Research paper thumbnail of PCR based molecular technique for identification and discrimination of quarantined and non-qurantined Tilletia sps

Research paper thumbnail of Unravelling the carbon and sulphur metabolism in coastal soil ecosystems using comparative cultivation-independent genome-level characterisation of microbial communities

PloS one, 2014

Bacterial autotrophy contributes significantly to the overall carbon balance, which stabilises at... more Bacterial autotrophy contributes significantly to the overall carbon balance, which stabilises atmospheric CO2 concentration and decelerates global warming. Little attention has been paid to different modes of carbon/sulphur metabolism mediated by autotrophic bacterial communities in terrestrial soil ecosystems. We studied these pathways by analysing the distribution and abundance of the diagnostic metabolic marker genes cbbM, apsA and soxB, which encode for ribulose-1,5-bisphosphate carboxylase/oxygenase, adenosine phosphosulphate reductase and sulphate thiohydrolase, respectively, among different contrasting soil types. Additionally, the abundance of community members was assessed by quantifying the gene copy numbers for 16S rRNA, cbbL, cbbM, apsA and soxB. Distinct compositional differences were observed among the clone libraries, which revealed a dominance of phylotypes associated with carbon and sulphur cycling, such as Gammaproteobacteria (Thiohalomonas, Allochromatium, Chroma...

Research paper thumbnail of Isolation and structural characterization of biosurfactant produced by an alkaliphilic bacterium Cronobacter sakazakii isolated from oil contaminated wastewater

Carbohydrate Polymers, 2000

Production of biosurfactant from an alkaliphilic bacterium Cronobacter sakazakii (Accession No.JN... more Production of biosurfactant from an alkaliphilic bacterium Cronobacter sakazakii (Accession No.JN398668) was screened by haemolytic assay, emulsifying activity and surface tension measurement. Biosurfactant, comprised of total sugars (73.3%), reducing sugars (1.464%), protein (11.9%), uronic acid (15.98%) and sulfate (6.015%), showed low viscosity with pseudoplastic rheological behavior and exhibited significant emulsification activity with oils and hydrocarbons. A series of low and

Research paper thumbnail of Isolation and temporal endospermal expression of γ-kafirin gene of grain sorghum (Sorghum bicolor L. moench) var. M 35-1 for introgression analysis of transgene

Journal of Cereal Science, 2008

Research paper thumbnail of Heterologous Expression of Legumin Gene in E-coli Isolated from cDNA Clones of Immature Seeds of Pigeonpea (Cajanus cajan L.)

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009

Research paper thumbnail of Cloning differentially expressed salt induced cDNAs from Dunaliella salina under super saturated salt stress using subtractive hybridization

Research paper thumbnail of Characterization of extracellular polymeric substances produced by micro-algae Dunaliella salina

Carbohydrate Polymers, 2011

Research paper thumbnail of Isolation and characterization of extracellular polymeric substances from micro-algae Dunaliella salina under salt stress

Bioresource Technology - BIORESOURCE TECHNOL, 2009

Extracellular polymeric substances (EPSs), produced by Dunaliella salina strain, increase concomi... more Extracellular polymeric substances (EPSs), produced by Dunaliella salina strain, increase concomitantly with salt concentration and maximum (944mg/l) were obtained at 5M NaCl, whereas minimum (56mg/l) at 0.5M salinity. Emulsifying activity was measured in terms of strength to retain the emulsion and comparatively 85.76% retention was observed at 0.5M salinity thereafter it intends to decline. The FT-IR-spectra reveal characteristic functional groups NH stretching, asymmetrical CH stretching vibration of aliphatic CH2-group, CC stretching of aromatic, CN stretch of aliphatic amine, NH wag of primary amine and CX stretch of alkyl-halides with a stretching of COC, CO corresponding to the presence of carbohydrates. The FT-IR-spectra substantiated the presence of primary amine-group, aromatic-compound, halide-group, aliphatic alkyl-group and polysaccharides. Four monosaccharides (glucose, galactose, fructose and xylose) were also detected by HPLC analysis. Production of EPSs may allow fu...

Research paper thumbnail of Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum

Research paper thumbnail of Bioinformatics: New directions to know facts of life

Bioinformatics is a scientific discipline of discovering biological functions through analysis of... more Bioinformatics is a scientific discipline of discovering biological functions through analysis of molecular sequence information from DNA, RNA or proteins. It is the computer assisted data management discipline that enables one to understand life's processes precisely. It helps to discover better drug target after differentiating between healthy and diseased. Bioinformatics is being considered bedrock for the future of biotechnology and is being adopted worldwide by academicians, companies, national and international consortia. Statistical and computational analysis of the complex relationships between phenotypes and genotypes; animals and plants plays a crucial role in the future of biology.

Research paper thumbnail of Bioinformatics and Its Application in Agri-biotechnology

Research paper thumbnail of Rheological Properties of Grain Sorghum Dough As Determined By Mixography

Research paper thumbnail of Studies on extensigraphic properties and effect of salt addition in grain sorghum dough

Research paper thumbnail of Differential distribution and abundance of diazotrophic bacterial communities across different soil niches using a gene‐targeted clone library approach

Research paper thumbnail of Non-targeted metabolomics and scavenging activity of reactive oxygen species reveal the potential of Salicornia brachiata as a functional food

Research paper thumbnail of Purification and characterization of cellulase from a marine< i> Bacillus</i> sp. H1666: A potential agent for single step saccharification of seaweed biomass

Research paper thumbnail of Ectopic over-expression of peroxisomal ascorbate peroxidase (< i> SbpAPX</i>) gene confers salt stress tolerance in transgenic peanut (< i> Arachis hypogaea</i>)

Research paper thumbnail of Microbial Exopolysaccharides

Research paper thumbnail of Extracellular polymeric substances from two biofilm forming< i> Vibrio</i> species: Characterization and applications

Research paper thumbnail of Production and structural characterization of biosurfactant produced by an alkaliphilic bacterium,< i> Klebsiella</i> sp.: Evaluation of different carbon sources

Research paper thumbnail of PCR based molecular technique for identification and discrimination of quarantined and non-qurantined Tilletia sps

Research paper thumbnail of Unravelling the carbon and sulphur metabolism in coastal soil ecosystems using comparative cultivation-independent genome-level characterisation of microbial communities

PloS one, 2014

Bacterial autotrophy contributes significantly to the overall carbon balance, which stabilises at... more Bacterial autotrophy contributes significantly to the overall carbon balance, which stabilises atmospheric CO2 concentration and decelerates global warming. Little attention has been paid to different modes of carbon/sulphur metabolism mediated by autotrophic bacterial communities in terrestrial soil ecosystems. We studied these pathways by analysing the distribution and abundance of the diagnostic metabolic marker genes cbbM, apsA and soxB, which encode for ribulose-1,5-bisphosphate carboxylase/oxygenase, adenosine phosphosulphate reductase and sulphate thiohydrolase, respectively, among different contrasting soil types. Additionally, the abundance of community members was assessed by quantifying the gene copy numbers for 16S rRNA, cbbL, cbbM, apsA and soxB. Distinct compositional differences were observed among the clone libraries, which revealed a dominance of phylotypes associated with carbon and sulphur cycling, such as Gammaproteobacteria (Thiohalomonas, Allochromatium, Chroma...

Research paper thumbnail of Isolation and structural characterization of biosurfactant produced by an alkaliphilic bacterium Cronobacter sakazakii isolated from oil contaminated wastewater

Carbohydrate Polymers, 2000

Production of biosurfactant from an alkaliphilic bacterium Cronobacter sakazakii (Accession No.JN... more Production of biosurfactant from an alkaliphilic bacterium Cronobacter sakazakii (Accession No.JN398668) was screened by haemolytic assay, emulsifying activity and surface tension measurement. Biosurfactant, comprised of total sugars (73.3%), reducing sugars (1.464%), protein (11.9%), uronic acid (15.98%) and sulfate (6.015%), showed low viscosity with pseudoplastic rheological behavior and exhibited significant emulsification activity with oils and hydrocarbons. A series of low and

Research paper thumbnail of Isolation and temporal endospermal expression of γ-kafirin gene of grain sorghum (Sorghum bicolor L. moench) var. M 35-1 for introgression analysis of transgene

Journal of Cereal Science, 2008

Research paper thumbnail of Heterologous Expression of Legumin Gene in E-coli Isolated from cDNA Clones of Immature Seeds of Pigeonpea (Cajanus cajan L.)

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009

Research paper thumbnail of Cloning differentially expressed salt induced cDNAs from Dunaliella salina under super saturated salt stress using subtractive hybridization

Research paper thumbnail of Characterization of extracellular polymeric substances produced by micro-algae Dunaliella salina

Carbohydrate Polymers, 2011

Log In