Ayşe Yüce - Academia.edu (original) (raw)
Papers by Ayşe Yüce
Mikrobiyoloji bülteni, 2009
Staphylococci are one of the most common pathogens isolated from nosocomial and community acquire... more Staphylococci are one of the most common pathogens isolated from nosocomial and community acquired infections. Antibiotics used by oral route such as erythromycin, clindamycin, trimethoprim-sulphamethoxazole (TMP-SMX) and quinolones are of value especially in the treatment of community acquired infections and resistance to those antibiotics may lead to therapeutic failure. Therefore in this study, susceptibility of staphylococci to TMP-SMX, rifampin, tetracycline, gentamicin, ciprofloxacin and vancomycin and the presence of inducible clindamycin resistance were investigated in two distinct university hospitals in Turkey. A total of 286 staphylococcus strains [184 Staphylococcus aureus, 102 coagulase negative staphylococci (CoNS)] were studied. Of the 90 hospital-acquired S. aureus, 44.6% were methicillin-resistant while all of the community acquired strains were methicillin-susceptible. All of the CoNS strains were isolated from nosocomial infections and 71.6% of them were resistant...
Mikrobiyoloji bülteni, 2003
The aim of this study was to investigate the IgG and IgM antibody positivities against atypical p... more The aim of this study was to investigate the IgG and IgM antibody positivities against atypical pneumonia agents in patients with community-acquired pneumonia (CAP), and to compare the results with the controls. The serum samples which were collected from 53 adult patients and 20 healthy donors have been investigated by a commercial indirect immunofluorescent assay (IFA, Pneumo-slide, Vircell SL, Spain) in which nine different antigens were fixed onto a slide. In both of the study groups. IgG and IgM seropositivities were detected in different rates against one or more etiologic agents. In the patient group. IgG and IgM positivity rates for the agents were as follows, respectively; 22.6% and 28.3% for Legionella pneumophila, 9.4% and 5.6% for Mycoplasma pneumoniae, 30.1% and 7.5% for Coxiella burnetii, 33.9% and 3.7% for Chlamydia pneumoniae, 28.3% and 0 for adenovirus, 71.6% and 1.8% for respiratory syncytial virus, 30.1% and 24.5% for influenza A virus, 35.8% and 7.5% for influenz...
Methicillin-resistant Staphylococcus aureus (MRSA), known as a nosocomial pathogen, has been isol... more Methicillin-resistant Staphylococcus aureus (MRSA), known as a nosocomial pathogen, has been isolated from community-acquired infections since the 1980s. It has been reported that there are carriers of MRSA in the community although the rate of carriers is low and the most important risk factor of community-acquired carriage is hospitalization or referral to healthcare facilities. We attempted to investigate methicillin-resistant and methicillin-susceptible S. aureus colonization, respectively, in nasal and axillary swabs obtained from 500 patients without a history of hospitalization who were admitted to outpatient clinics and from 102 healthcare workers chosen as a control group. Of the patients, 9.4% had nasal S. aureus colonization without methicillin-resistant strains. Of the health care workers, 8.8% had S. aureus colonization without methicillin-resistant strains and only one worker had MRSA. The nasal carriage ratio of S. aureus in children was found to be 19.1% (22 of 115), and that in adults was 6.5% (25 of 385). The difference between the two age groups was determined as statistically significant (P = 0.006).
Mikrobiyoloji Bulteni, 2013
Dang ateşi, tropikal ve subtropikal ülkelerde tüm yaş gruplarını etkileyen akut viral bir hastalı... more Dang ateşi, tropikal ve subtropikal ülkelerde tüm yaş gruplarını etkileyen akut viral bir hastalıktır. Vektörü ağırlıklı olarak, Aedes aegypti ve A.albopictus cinsi sivrisineklerdir. Ülkemizde dang ateşi tanısı almış bir olgu bildirimine rastlanmamış olmakla birlikte, seroepidemiyolojik çalışmalar Türkiye'de Dengue virus (DENV) varlığına işaret etmektedir. Bu raporda, yurtdışından gelen bir dang ateşi olgusu sunulmuştur. Bilinen bir hastalığı olmayan İsviçre vatandaşı 40 yaşında bir erkek hasta 15 gün önce Dubai'den Hindistan'a göç etmiş, bir hafta sonra yüksek ateş nedeniyle Hindistan'da bir kliniğe başvurmuştur. Burada yapılan NS1 antijen testinde (Bio-Rad Laboratories, ABD) pozitifliğin saptanması üzerine hastaya dang ateşi tanısı konularak izleme alınmıştır. Hasta izlem altındayken ziyaret için Türkiye'ye geldiğinde kliniğimize kontrol amaçlı başvuruda bulunmuştur. Yapılan hemogram incelemesinde trombositopeni (PLT: 48.000/µl), lökopeni (beyaz küre: 2800 /µl) ve karaciğer enzimlerinde yükselme (AST: 76 U/L, ALT: 83 U/L) saptanması üzerine hasta kliniğimize yatırılmıştır. Hastanın izleminde ateş tespit edilmemiş; bilateral alt ekstremitelerde deride peteşiyal döküntü dışında fizik muayenesi doğal bulunmuştur. Beyaz küre sayısı 4100/µl'ye, trombosit değeri 93.000/µl'ye yükselen hastanın karaciğer fonksiyon testleri, yatışının üçüncü gününde gerilemiş (AST: 63 U/L, ALT: 78 U/L) ve trombosit değerinin 150.000/µl olduğu saptanmıştır. Üç günlük izleminde ateşi olmayan, laboratuvar bulguları normale dönen ve genel durumu iyi olan hasta önerilerle taburcu edilmiştir. Hastanın serum örneği dang ateşi ke-Geliş Tarihi (
Vaccine, 2007
A serological survey to determine the immunity to diphtheria in the adult population in Izmir-Tur... more A serological survey to determine the immunity to diphtheria in the adult population in Izmir-Turkey was conducted according to the recommendations of the World Health Organization. A total of 339 blood samples were collected from subjects aged between 20 and 81, and diphtheria antitoxin levels were measured by the enzyme linked immunosorbent assay method. Titers below 0.1 IU/ml were considered to show insufficient immunity. Of the studied population, 56.3% had showed insufficient immunity against diphtheria. Diphtheria protection rates showed a gradual age-related decrease. The lowest immunity rate was observed in 40-49-year age group (30.6%) (p < 0.05). There was no significant difference in the rate of immunity between subjects in terms of sex, levels of education, urban-rural region (p > 0.05). These results emphasize the need for booster immunization of adults. The present vaccination policy should include re-vaccinations of the adult population every 10 years in order to provide a complete protection of the population.
Mycoses, 2006
In recent years, a progressive increase in the frequency of nosocomial candidaemia has been obser... more In recent years, a progressive increase in the frequency of nosocomial candidaemia has been observed, especially among the critically ill or immunocompromised patients. The aim of this study was to evaluate the trend in incidence of candidaemia together with potential risk factors in an 850-bed Turkish Tertiary Care Hospital in a 4-year period. A total of 104 candidaemia episodes were identified in 104 patients. The overall incidence was 0.56 per 1000 hospital admissions and the increase in incidence of candidaemia from 2000 to 2003 was found to be statistically significant (P = 0.010). Candida albicans was the most common species (57.7%) and non-albicans species accounted for 42.3% of all episodes. The most common non-albicans Candida sp. isolated was C. tropicalis (20.2%) followed by C. parapsilosis (12.5%). The most frequent risk factors possibly associated with the candidaemia were previous antibiotic treatment (76.9%), presence of central venous catheter (71.2%) and total parenteral nutrition (55.8%). Our results show the fact that the incidence of candidaemia caused by non-albicans species is frequent and increasing significantly, although the most common isolated Candida species were C. albicans and further investigations are necessary to evaluate the mechanisms of increasing incidence of candidaemia caused by non-albicans species.
International Journal of Infectious Diseases, 2005
International Journal of Infectious Diseases, 2006
Infection Control and Hospital Epidemiology, 2007
To evaluate the specificity and sensitivity of the clinical criteria widely used to differentiate... more To evaluate the specificity and sensitivity of the clinical criteria widely used to differentiate true coagulase-negative staphylococcal (CoNS) bacteremia from contamination, using pulsed-field gel electrophoresis (PFGE) as the reference test. The study sample consisted of 79 CoNS isolates recovered from cultures of blood from 38 patients. Medical charts of the patients were reviewed for demographic and clinical information. The relatedness of CoNS strains recovered from 2 or more successive blood cultures was analyzed by PFGE. Patients from whom similar strains were recovered were assumed to have true bacteremia, whereas patients from whom different strains were recovered were considered to have contaminated blood cultures. The clinical criteria comprised Centers for Disease Control and Prevention (CDC) surveillance definitions for bloodstream infection (BSI), as well as an alternative criterion based on the presence of fever, the presence of leukocytosis, the absence of another recognized infection, and the recovery of CoNS from 2 or more successive blood cultures. Nineteen (50%) of the 38 patients had bacteremia due to similar strains; the remaining patients had bacteremia due to different strains. Criterion 2a of the CDC definition for BSI had a sensitivity of 100% and a specificity of 31.6% for distinguishing between true bacteremia and contamination. CDC criterion 2b had a sensitivity of 78.9% and a specificity of 52.6%. Molecular typing correlated poorly with the clinical criteria for true bacteremia. In view of the limited applicability of clinical criteria, more studies are needed to improve them. Periodic cross-sectional studies based on PFGE findings might be useful to estimate local contamination rates in an institution, which in turn can be used to improve the accuracy of the clinical diagnosis of bacteremia.
American Journal of Infection Control, 2008
The major concern after occupational exposures is the possible transmission of blood-borne pathog... more The major concern after occupational exposures is the possible transmission of blood-borne pathogens, especially hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). This study was undertaken to evaluate the risk of infection after exposure to blood or body fluids of an unknown or an HBV-, HCV-, and HIV-negative source and to determine the epidemiologic characteristics of these incidents in health care workers. The survey was conducted over a 6-year period at a university hospital in Turkey, using a questionnaire to elicit demographic and epidemiologic information. Serologic tests for HBV, HCV, and HIV were performed and repeated after 3 months. Of the 449 incidents, complete follow-up was achieved in 320 (71.3%), and no seroconversion was observed for HBV, HCV and HIV. Most of the incidents occurred in medical (34.7%) and surgical (25.4%) work areas. The most frequent type of exposure was percutaneous injury (94%), most commonly caused by handling of garbage bags (58.4%), needle recapping (16.5%), and invasive interventions (13.4%). Infection risk seems to be extremely low for HCV and HIV, because of low endemicity, and for HBV in groups immunized against HBV.
Mikrobiyoloji bülteni, 2009
Staphylococci are one of the most common pathogens isolated from nosocomial and community acquire... more Staphylococci are one of the most common pathogens isolated from nosocomial and community acquired infections. Antibiotics used by oral route such as erythromycin, clindamycin, trimethoprim-sulphamethoxazole (TMP-SMX) and quinolones are of value especially in the treatment of community acquired infections and resistance to those antibiotics may lead to therapeutic failure. Therefore in this study, susceptibility of staphylococci to TMP-SMX, rifampin, tetracycline, gentamicin, ciprofloxacin and vancomycin and the presence of inducible clindamycin resistance were investigated in two distinct university hospitals in Turkey. A total of 286 staphylococcus strains [184 Staphylococcus aureus, 102 coagulase negative staphylococci (CoNS)] were studied. Of the 90 hospital-acquired S. aureus, 44.6% were methicillin-resistant while all of the community acquired strains were methicillin-susceptible. All of the CoNS strains were isolated from nosocomial infections and 71.6% of them were resistant...
Mikrobiyoloji bülteni, 2003
The aim of this study was to investigate the IgG and IgM antibody positivities against atypical p... more The aim of this study was to investigate the IgG and IgM antibody positivities against atypical pneumonia agents in patients with community-acquired pneumonia (CAP), and to compare the results with the controls. The serum samples which were collected from 53 adult patients and 20 healthy donors have been investigated by a commercial indirect immunofluorescent assay (IFA, Pneumo-slide, Vircell SL, Spain) in which nine different antigens were fixed onto a slide. In both of the study groups. IgG and IgM seropositivities were detected in different rates against one or more etiologic agents. In the patient group. IgG and IgM positivity rates for the agents were as follows, respectively; 22.6% and 28.3% for Legionella pneumophila, 9.4% and 5.6% for Mycoplasma pneumoniae, 30.1% and 7.5% for Coxiella burnetii, 33.9% and 3.7% for Chlamydia pneumoniae, 28.3% and 0 for adenovirus, 71.6% and 1.8% for respiratory syncytial virus, 30.1% and 24.5% for influenza A virus, 35.8% and 7.5% for influenz...
Methicillin-resistant Staphylococcus aureus (MRSA), known as a nosocomial pathogen, has been isol... more Methicillin-resistant Staphylococcus aureus (MRSA), known as a nosocomial pathogen, has been isolated from community-acquired infections since the 1980s. It has been reported that there are carriers of MRSA in the community although the rate of carriers is low and the most important risk factor of community-acquired carriage is hospitalization or referral to healthcare facilities. We attempted to investigate methicillin-resistant and methicillin-susceptible S. aureus colonization, respectively, in nasal and axillary swabs obtained from 500 patients without a history of hospitalization who were admitted to outpatient clinics and from 102 healthcare workers chosen as a control group. Of the patients, 9.4% had nasal S. aureus colonization without methicillin-resistant strains. Of the health care workers, 8.8% had S. aureus colonization without methicillin-resistant strains and only one worker had MRSA. The nasal carriage ratio of S. aureus in children was found to be 19.1% (22 of 115), and that in adults was 6.5% (25 of 385). The difference between the two age groups was determined as statistically significant (P = 0.006).
Mikrobiyoloji Bulteni, 2013
Dang ateşi, tropikal ve subtropikal ülkelerde tüm yaş gruplarını etkileyen akut viral bir hastalı... more Dang ateşi, tropikal ve subtropikal ülkelerde tüm yaş gruplarını etkileyen akut viral bir hastalıktır. Vektörü ağırlıklı olarak, Aedes aegypti ve A.albopictus cinsi sivrisineklerdir. Ülkemizde dang ateşi tanısı almış bir olgu bildirimine rastlanmamış olmakla birlikte, seroepidemiyolojik çalışmalar Türkiye'de Dengue virus (DENV) varlığına işaret etmektedir. Bu raporda, yurtdışından gelen bir dang ateşi olgusu sunulmuştur. Bilinen bir hastalığı olmayan İsviçre vatandaşı 40 yaşında bir erkek hasta 15 gün önce Dubai'den Hindistan'a göç etmiş, bir hafta sonra yüksek ateş nedeniyle Hindistan'da bir kliniğe başvurmuştur. Burada yapılan NS1 antijen testinde (Bio-Rad Laboratories, ABD) pozitifliğin saptanması üzerine hastaya dang ateşi tanısı konularak izleme alınmıştır. Hasta izlem altındayken ziyaret için Türkiye'ye geldiğinde kliniğimize kontrol amaçlı başvuruda bulunmuştur. Yapılan hemogram incelemesinde trombositopeni (PLT: 48.000/µl), lökopeni (beyaz küre: 2800 /µl) ve karaciğer enzimlerinde yükselme (AST: 76 U/L, ALT: 83 U/L) saptanması üzerine hasta kliniğimize yatırılmıştır. Hastanın izleminde ateş tespit edilmemiş; bilateral alt ekstremitelerde deride peteşiyal döküntü dışında fizik muayenesi doğal bulunmuştur. Beyaz küre sayısı 4100/µl'ye, trombosit değeri 93.000/µl'ye yükselen hastanın karaciğer fonksiyon testleri, yatışının üçüncü gününde gerilemiş (AST: 63 U/L, ALT: 78 U/L) ve trombosit değerinin 150.000/µl olduğu saptanmıştır. Üç günlük izleminde ateşi olmayan, laboratuvar bulguları normale dönen ve genel durumu iyi olan hasta önerilerle taburcu edilmiştir. Hastanın serum örneği dang ateşi ke-Geliş Tarihi (
Vaccine, 2007
A serological survey to determine the immunity to diphtheria in the adult population in Izmir-Tur... more A serological survey to determine the immunity to diphtheria in the adult population in Izmir-Turkey was conducted according to the recommendations of the World Health Organization. A total of 339 blood samples were collected from subjects aged between 20 and 81, and diphtheria antitoxin levels were measured by the enzyme linked immunosorbent assay method. Titers below 0.1 IU/ml were considered to show insufficient immunity. Of the studied population, 56.3% had showed insufficient immunity against diphtheria. Diphtheria protection rates showed a gradual age-related decrease. The lowest immunity rate was observed in 40-49-year age group (30.6%) (p < 0.05). There was no significant difference in the rate of immunity between subjects in terms of sex, levels of education, urban-rural region (p > 0.05). These results emphasize the need for booster immunization of adults. The present vaccination policy should include re-vaccinations of the adult population every 10 years in order to provide a complete protection of the population.
Mycoses, 2006
In recent years, a progressive increase in the frequency of nosocomial candidaemia has been obser... more In recent years, a progressive increase in the frequency of nosocomial candidaemia has been observed, especially among the critically ill or immunocompromised patients. The aim of this study was to evaluate the trend in incidence of candidaemia together with potential risk factors in an 850-bed Turkish Tertiary Care Hospital in a 4-year period. A total of 104 candidaemia episodes were identified in 104 patients. The overall incidence was 0.56 per 1000 hospital admissions and the increase in incidence of candidaemia from 2000 to 2003 was found to be statistically significant (P = 0.010). Candida albicans was the most common species (57.7%) and non-albicans species accounted for 42.3% of all episodes. The most common non-albicans Candida sp. isolated was C. tropicalis (20.2%) followed by C. parapsilosis (12.5%). The most frequent risk factors possibly associated with the candidaemia were previous antibiotic treatment (76.9%), presence of central venous catheter (71.2%) and total parenteral nutrition (55.8%). Our results show the fact that the incidence of candidaemia caused by non-albicans species is frequent and increasing significantly, although the most common isolated Candida species were C. albicans and further investigations are necessary to evaluate the mechanisms of increasing incidence of candidaemia caused by non-albicans species.
International Journal of Infectious Diseases, 2005
International Journal of Infectious Diseases, 2006
Infection Control and Hospital Epidemiology, 2007
To evaluate the specificity and sensitivity of the clinical criteria widely used to differentiate... more To evaluate the specificity and sensitivity of the clinical criteria widely used to differentiate true coagulase-negative staphylococcal (CoNS) bacteremia from contamination, using pulsed-field gel electrophoresis (PFGE) as the reference test. The study sample consisted of 79 CoNS isolates recovered from cultures of blood from 38 patients. Medical charts of the patients were reviewed for demographic and clinical information. The relatedness of CoNS strains recovered from 2 or more successive blood cultures was analyzed by PFGE. Patients from whom similar strains were recovered were assumed to have true bacteremia, whereas patients from whom different strains were recovered were considered to have contaminated blood cultures. The clinical criteria comprised Centers for Disease Control and Prevention (CDC) surveillance definitions for bloodstream infection (BSI), as well as an alternative criterion based on the presence of fever, the presence of leukocytosis, the absence of another recognized infection, and the recovery of CoNS from 2 or more successive blood cultures. Nineteen (50%) of the 38 patients had bacteremia due to similar strains; the remaining patients had bacteremia due to different strains. Criterion 2a of the CDC definition for BSI had a sensitivity of 100% and a specificity of 31.6% for distinguishing between true bacteremia and contamination. CDC criterion 2b had a sensitivity of 78.9% and a specificity of 52.6%. Molecular typing correlated poorly with the clinical criteria for true bacteremia. In view of the limited applicability of clinical criteria, more studies are needed to improve them. Periodic cross-sectional studies based on PFGE findings might be useful to estimate local contamination rates in an institution, which in turn can be used to improve the accuracy of the clinical diagnosis of bacteremia.
American Journal of Infection Control, 2008
The major concern after occupational exposures is the possible transmission of blood-borne pathog... more The major concern after occupational exposures is the possible transmission of blood-borne pathogens, especially hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). This study was undertaken to evaluate the risk of infection after exposure to blood or body fluids of an unknown or an HBV-, HCV-, and HIV-negative source and to determine the epidemiologic characteristics of these incidents in health care workers. The survey was conducted over a 6-year period at a university hospital in Turkey, using a questionnaire to elicit demographic and epidemiologic information. Serologic tests for HBV, HCV, and HIV were performed and repeated after 3 months. Of the 449 incidents, complete follow-up was achieved in 320 (71.3%), and no seroconversion was observed for HBV, HCV and HIV. Most of the incidents occurred in medical (34.7%) and surgical (25.4%) work areas. The most frequent type of exposure was percutaneous injury (94%), most commonly caused by handling of garbage bags (58.4%), needle recapping (16.5%), and invasive interventions (13.4%). Infection risk seems to be extremely low for HCV and HIV, because of low endemicity, and for HBV in groups immunized against HBV.