Ayesha Nadeem - Academia.edu (original) (raw)

Papers by Ayesha Nadeem

Biomedical Journal of Scientific & Technical Research, 2021

Moringa oleifera Lam. (Sohanjna, Drumstick tree, Horseradish tree; family, Moringaceae) is a well... more Moringa oleifera Lam. (Sohanjna, Drumstick tree, Horseradish tree; family, Moringaceae) is a well-known plant, native to Pakistan, Bangladesh, India, Sri Lanka, Philippines and Africa. It is known as miracle tree as well as mother's best friend. Almost every part of plant (i.e., leaves, seeds, bark, root and wood) is a rich source of vitamins, minerals (calcium, magnesium and phosphorus), carotenes and folic acid. Various parts of M. oleifera such as pods, roots and bark have been reported for kidney stone disease; however, no such data is available to show the antiurolithiatic potential of seeds. The aim of the study was to validate the antiurolithiatic potential of Moringa oleifera Lam. seeds against ethylene glycol-induced urolithiasis. Aqueous alcoholic extract of Moringa oleifera Lam. seeds (MoS.Cr) was prepared and phytochemical screening was carried out to assess the medicinally important phytoconstituents. To evaluate the antiurolithic potential, Wistar albino male rats were divided into different groups. All the groups except normal control group received lithogenic treatment (1% ammonium chloride +0.75% EG) for 21 days. Normal control and intoxicated groups received D. W (5ml/kg; p.o.), treatment groups received MoS. Cr at the doses of 100, 300 and 500mg/kg; p.o. while the standard group received cystone (500mg/kg; p.o.) for the next 14 days. After 21 and 35 days, animals were placed individually in metabolic cages and urine samples were analyzed for different parameters, i.e., crystal count, urine pH, urine volume, uric acid, calcium, magnesium, phosphorus and total protein levels. After the completion of study (at 35th day), blood samples were collected via retro-orbital technique, serum was separated and analyzed to determine serum creatinine and blood urea nitrogen levels. Histological studies were performed to evaluate the protective effects of MoS.Cr against ethylene glycolinduced urolithiasis. MoS.Cr was found safe upto the dose of 10 g/kg when tested for toxicity. Phytochemical screening revealed the presence of alkaloids, phenols, flavonoids, tannins, saponins, glycosides and coumarins. MoS.Cr showed dose-dependent effects and restored the biochemical parameters towards normal. The results conclude that the seeds of M. oleifera possess stone inhibiting potential mediated possibly due to antioxidant potential, urine alkalinizing and diuretic effects, thus providing scientific background for its use against urolithiasis.

Biomedical Journal of Scientific & Technical Research, 2021

Moringa oleifera Lam. (Sohanjna, Drumstick tree, Horseradish tree; family, Moringaceae) is a well... more Moringa oleifera Lam. (Sohanjna, Drumstick tree, Horseradish tree; family, Moringaceae) is a well-known plant, native to Pakistan, Bangladesh, India, Sri Lanka, Philippines and Africa. It is known as miracle tree as well as mother's best friend. Almost every part of plant (i.e., leaves, seeds, bark, root and wood) is a rich source of vitamins, minerals (calcium, magnesium and phosphorus), carotenes and folic acid. Various parts of M. oleifera such as pods, roots and bark have been reported for kidney stone disease; however, no such data is available to show the antiurolithiatic potential of seeds. The aim of the study was to validate the antiurolithiatic potential of Moringa oleifera Lam. seeds against ethylene glycol-induced urolithiasis. Aqueous alcoholic extract of Moringa oleifera Lam. seeds (MoS.Cr) was prepared and phytochemical screening was carried out to assess the medicinally important phytoconstituents. To evaluate the antiurolithic potential, Wistar albino male rats were divided into different groups. All the groups except normal control group received lithogenic treatment (1% ammonium chloride +0.75% EG) for 21 days. Normal control and intoxicated groups received D. W (5ml/kg; p.o.), treatment groups received MoS. Cr at the doses of 100, 300 and 500mg/kg; p.o. while the standard group received cystone (500mg/kg; p.o.) for the next 14 days. After 21 and 35 days, animals were placed individually in metabolic cages and urine samples were analyzed for different parameters, i.e., crystal count, urine pH, urine volume, uric acid, calcium, magnesium, phosphorus and total protein levels. After the completion of study (at 35th day), blood samples were collected via retro-orbital technique, serum was separated and analyzed to determine serum creatinine and blood urea nitrogen levels. Histological studies were performed to evaluate the protective effects of MoS.Cr against ethylene glycolinduced urolithiasis. MoS.Cr was found safe upto the dose of 10 g/kg when tested for toxicity. Phytochemical screening revealed the presence of alkaloids, phenols, flavonoids, tannins, saponins, glycosides and coumarins. MoS.Cr showed dose-dependent effects and restored the biochemical parameters towards normal. The results conclude that the seeds of M. oleifera possess stone inhibiting potential mediated possibly due to antioxidant potential, urine alkalinizing and diuretic effects, thus providing scientific background for its use against urolithiasis.