Ayodeji Olabiyi - Academia.edu (original) (raw)

Papers by Ayodeji Olabiyi

Basic & Clinical Pharmacology & Toxicology, 2007

The present study was carried out to evaluate the ameliorative effects of kolaviron (a biflavonoi... more The present study was carried out to evaluate the ameliorative effects of kolaviron (a biflavonoid from the seeds of Garcinia kola) and curcumin (from the rhizome, Curcuma longa L.) on the di-n-butylphthalate (DBP)-induced testicular damage in rats. Administration of DBP to rats at a dose of 2 g/kg for 9 days significantly decreased the relative testicular weights compared to the controls, while the weights of other organs remained unaffected. Curcumin or kolaviron did not affect all the organ weights of the animals. While only DBP treatment significantly increased the testicular malondialdehyde level and gamma-glutamyl transferase activity (gamma-GT), it markedly decreased glutathione level, the testicular catalase, glucose-6-phosphate dehydrogenase, superoxide dismutase, sperm gamma-GT activities and serum testosterone level compared to the control group. Data on cauda epididymal sperm count and live/dead ratio were not significantly affected in the DBP-treated rats. Alone, DBP treatment resulted in a 66% decrease in spermatozoa motility and a 77% increase in abnormal spermatozoa in comparison to control. DBP-treated rats showed marked degeneration of the seminiferous tubules with necrosis and defoliation of spermatocytes. The DBP-induced injuries in biochemical, spermatological parameters and histological structure of testis were recovered by treatment with kolaviron or curcumin. The pattern in the behaviour of these compounds might be correlated with their structural variations. Our results indicate that kolaviron and curcumin protect against testicular oxidative damage induced by DBP. The chemoprotective effects of these compounds may be due to their intrinsic antioxidant properties and as such may prove useful in combating phthalate-induced reproductive toxicity.

Redox Report, 2016

Objectives: Exposure to 4-vinylcyclohexene diepoxide (VCD) was reported to induce testicular germ... more Objectives: Exposure to 4-vinylcyclohexene diepoxide (VCD) was reported to induce testicular germ cell toxicity in rodents. However, there is paucity of information on the precise biochemical and molecular mechanisms of VCD-induced male reproductive toxicity. Methodology: This study investigated the influence of VCD on testicular and epidydimal functions following oral exposure of Wistar rats to VCD at 0, 100, 250 and 500 mg/kg for 28 consecutive days. Results: Administration of VCD significantly decreased the body weight gain and organosomatic indices of the testes and epididymis. When compared with the control, VCD significantly decreased superoxide dismutase and catalase activities in the testes whereas it significantly decreased superoxide dismutase activity but increased catalase activity in the epididymis. Moreover, while glutathione peroxidase activity and glutathione level remain unaffected, exposure of rats to VCD significantly increased glutathione S-transferase activity as well as hydrogen peroxide and malondialdehyde levels in testes and epididymis of the treated rats. The spermiogram of VCD-treated rats showed significant decrease in epididymal sperm count, sperm progressive motility, testicular sperm number and daily sperm production when compared with the control. Administration of VCD significantly decreased circulatory concentrations of follicle-stimulating hormone, luteinizing hormone and testosterone along with testicular and epididymal degeneration in the treated rats. Immunohistochemical analysis showed significantly increased cyclooxygenase-2, inducible nitric oxide synthase, caspase-9 and caspase-3 protein expressions in the testes of VCDtreated rats. Conclusion: Exposure to VCD induces testicular and epidydimal dysfunctions via endocrine suppression, disruption of antioxidant enzymes activities, increase in biomarkers of oxidative stress, inflammation and apoptosis in rats.

Ayodele Jacob Akinyemi Increasing amounts of evidence suggests tannic acid (TA) and its derivativ... more Ayodele Jacob Akinyemi Increasing amounts of evidence suggests tannic acid (TA) and its derivative, gallic acid (GA) has beneficial effects against nephrotoxicity. The aim of this study was to investigate the effect of TA and GA (two structurally related phenolic acids) against cisplatin-induced thiobarbituric acid reactive substances (TBARS) production in rat kidney in vitro. The effect of both phenolic acids on kidney TBARS level in the presence of cisplatin, an antineoplastic drug used in the treatment of many solid-tissue cancers which has its chief side effect in nephrotoxicity, was assessed. Thereafter, the antioxidant properties were also determined. Incubation of the kidney tissue homogenate in the presence of cisplatin (1 mM) caused a significant (P<0.05) increase in the TBARS level. However, both phenolic acids caused a significant (P<0.05) decrease in the TBARS content of the kidney in a dose dependent manner (0 – 12.5 μM). Nevertheless, TA had a significantly highe...

Anatomy journal of Africa, 2017

Neurotoxicity of 3, 4-Methylenedioxymethamphetamine (MDMA, ecstasy) is still controversially disc... more Neurotoxicity of 3, 4-Methylenedioxymethamphetamine (MDMA, ecstasy) is still controversially discussed. MDMA is an amphetamine derivative that has gained significant popularity in recent years and has become the recreational drug of choice for many young, adolescents and adults. This study sought to investigate that MDMA causes a behavioral, biochemical and neurocytoarchitechural change in male adolescent mice. Fifteen adolescent male mice were used for this study. They were randomly divided into 3 groups, of five mice each, control group received normal saline (Ctrl), 5 mice received 10 mg/kg of MDMA, s.c (LoD) and another 5 mice received 20 mg/kg of MDMA, s.c (HiD). The animals were subjected to behavioural tests to check their motor activity. Biochemical analysis to test for the level of catalase and monoamine oxidase activity was also done. The motor activity of animals treated with 10 mg/kg and 20 mg/kg of MDMA was significantly (p < 0.05) lower when compared with the contro...

Unripe pawpaw plant is known to be very rich in bioactive phytochemicals and its fruits have been... more Unripe pawpaw plant is known to be very rich in bioactive phytochemicals and its fruits have been an important ingredient in folklore medicine for the treatment of diabetes mellitus, but there is limited information on the possible mechanism of action by which it exerts this therapeutic effect.From this research,unripe pawpaw parts are rich sources of polyphenolics such as sinapinic acid, 2-phenyl-6I-O-beta-D-glucoside, O-coumaric acid, epicatechin, quercetin, luteolin, ferulic acid, kaempferol and caffeic acid; and alkaloid such as carpaine with inhibitory activities against key enzyme linked to type 2 diabetes (α-amylase and α-glucosidase).

Journal of food biochemistry, 2021

This study examined the behavioral responses, purinergic receptor densities, ectonucleotidases (E... more This study examined the behavioral responses, purinergic receptor densities, ectonucleotidases (E-NTPDase), adenosine deaminase (ADA) activity, and nitric oxide (NO) levels along with the parameters of oxidative stress-related to erectile function in the cerebral cortex (CC) of L-NAME-challenged rats pretreated with tigernut (TN) and walnut (WN) dietary supplementation. Wistar rats (male) of 70 total animals (250-300 g) were used in this research and hence separated into seven groups (n = 10): Group I: normal control-fed basal diet; Group II: positive control-fed basal diet/L-NAME/Sildenafil citrate (5 mg kg-1 day-1 ); Group III: ED-induced (placed on a basal diet/L-NAME); Group IV: diet supplemented with processed TN (20%)/L-NAME; Group V: diet supplemented with raw TN (20%)/L-NAME; Group VI: diet supplemented with processed WN (20%)/L-NAME; and Group VII placed on a diet supplemented with raw WN (20%)/L-NAME. The rats were pretreated for 2 weeks before the L-NAME (40 mg kg-1 day...

Journal of Food Biochemistry

In order to confirm the erectile potential of Quercetin (Q) in cyclosporine-induced hypertensive ... more In order to confirm the erectile potential of Quercetin (Q) in cyclosporine-induced hypertensive rats, this research assesses the influence of Q on the ectonucleotidases and adenosine deaminase (ADA) in the brains of rats. Male Wistar rats (200 g-250 g) were divided into five groups at random: Normal control (CTRL), cyclosporine-induced hypertensive rats (25 mg kg day-1 ) (HT) group, positive control (Sildenafil [SIL], 5 mg kg day-1 ), Quercetin 25 mg kg day-1 (25 Q), and Quercetin 50 mg kg day-1 (50 Q). Aside from standard diet-fed male rats; cyclosporine was given i.p for the period of 30 days as well as Q orally while the female rats were only given a standard diet. The animals were subjected to sexual activity (copulation) after which the male rat hypothalamus was dissected for biochemical examination (E-NTPDase activities, ecto-5'-nucleotidase as well as ADA and also levels of nitric oxide [NO]). We observed that Q enhanced copulatory behavior as evident in mounting, intromission, ejaculation numbers, and latencies. A substantial (p < .05) increase in the activity of E-NTPDase (ATP and ADP as substrate) without any notable difference in the action of ecto-5' nucleotidase was facilitated by cyclosporine-induction when compared to the CTRL. The 50 mg/kg, however, had the highest reversal effect in accordance with dose manner. Also, cyclosporine increased ADA activity with a concomitant reduction of NO level while both doses of Q down-regulated ADA activity and, increased NO levels. Enhanced sexual behavior, modulation of ectonucleotidases as well as ADA activity and increased NO levels suggest that Q-rich plant foods may be promising sources of dietary phytonutrients for erectile dysfunction (ED) management. PRACTICAL APPLICATIONS: Behavioral and biochemical assays evaluated showed that Q significantly enhanced sexual behavior as well as improved ATP bioavailability in cyclosporine-induced erectile dysfunctional rats. The modulatory effects of Q on ectonucleotidases, along with its ability to minimize adenosine deaminase activity and increase nitric oxide levels, indicate that Q-rich plants and/or plant foods may be promising sources of dietary phytonutrients for erectile dysfunction management.

Male sexual dysfunction (MSD) could be caused by various factors which include psychological diso... more Male sexual dysfunction (MSD) could be caused by various factors which include psychological disorders, androgen deficiencies, chronic medical conditions, vascular insufficiency, penile disease, pelvic surgery, neurological disorders, drugs, life style, aging and systemic diseases. This study sought to assess the antioxidant properties of the aqueous extracts of the leaves of Black plum (Vitex doniana) and bark of Kola nut (Cola nitida),evaluate their effect on pro-oxidant induced lipid peroxidation in rat’s testes; and investigate the effect on arginase which is one of the key enzymes linked to Erectile dysfunction. The results of the total phenol, total flavonoid of aqueous extracts of Cola nitida and Vitex doniana revealed that Cola nitida (10.64 mgGAE/g) had significantly (P

Nutrients

In hypertensive individuals, platelet morphology and function have been discovered to be altered,... more In hypertensive individuals, platelet morphology and function have been discovered to be altered, and this has been linked to the development of vascular disease, including erectile dysfunction (ED). The impact of nutritional supplementation with Cyperus esculentus (tiger nut, TN) and Tetracarpidium conophorum (walnut, WN) on androgen levels, ectonucleotidases, and adenosine deaminase (ADA) activities in platelets from L-NAME (Nω-nitro-L-arginine methyl ester hydrochloride) challenged rats were investigated. We hypothesized that these nuts may show a protective effect on platelets aggregation and possibly enhance the sex hormones, thereby reverting vasoconstriction. Wistar rats (male; 250–300 g; n = 10) were grouped into seven groups as follows: basal diet control group (I); basal diet/L-NAME/Viagra (5 mg/kg/day) as positive control group (II); ED-induced group (basal diet/L-NAME) (III); diet supplemented processed TN (20%)/L-NAME (IV); diet supplemented raw TN (20%)/L-NAME (V); die...

Drug Metabolism and Personalized Therapy

Objectives Hyperinsulinemia increases the risk factor of diabetes and infertility at a manifold. ... more Objectives Hyperinsulinemia increases the risk factor of diabetes and infertility at a manifold. Lactobacillus plantarum has several medical significances with limited reports. Hence, this study assessed the effect of L. plantarum on sexual-reproductive functions and distribution of insulin receptors in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice. Methods Forty male adult mice were divided into five groups as follows: control, high-fat diet (HFD) + streptozotocin (STZ), therapeutic, co-administration group type 1 (CO-AD) and probiotics. They were either simultaneously exposed to an HFD and L. plantarum treatment for 28 days with a dose of STZ injection to induce hyperinsulinemia on day 28 or treated with L. plantarum for 14 days, and following induction of hyperinsulinemia. Mice were subjected to a sexual behavioural test and thereafter sacrificed under euthanasia condition. Blood, brain and testes were collected for biochemical and immunohistochemical assays...

NIgerian Journal of Neuroscience

This study investigated the inhibitory effect of Cyperus esculentus L. (raw and processed) tubers... more This study investigated the inhibitory effect of Cyperus esculentus L. (raw and processed) tubers on Iron (Fe2+) induced lipid peroxidation and key enzymes linked with neurodegeneration in vitro. Ten grams of tiger nut tubers was weighed and added to 100 mL of distilled water (1:10 w/v), left for 24 h, filtered, centrifuged to obtain a clear supernatant and lyophilized. Activities of acetyl cholinesterase (AChE), butyryl cholinesterase (BChE), Fe2+-induced lipid peroxidation as well as antioxidants status as indicated by 2,2-azino-bis (3-ethylbenthiazoline-6-sulphonic acid radical scavenging ability and Fe chelation were evaluated. Raw tiger nut extracts inhibited activities of AChE and BChE in the brain tissue of rats (0 – 11.54 mg /mL) with EC50 = 3.599 mg /mL and 2.71 ± 0.01 mg /mL respectively, and increased antioxidant activity when compared with the processed tuber. Inhibition on these enzymes as well as prevention of Fe2+ induced lipid peroxidation may be the possible mechani...

Food and Chemical Toxicology

Redox report : communications in free radical research, 2017

This study investigated the potential anti-inflammatory effect of hesperidin against carrageenan ... more This study investigated the potential anti-inflammatory effect of hesperidin against carrageenan induced pleurisy in rat model. Twenty-four adult female Wistar rats (350 - 450g) were grouped as follows: Group I: rats were administered saline solution only (Normal control group); Group II: rats were administered saline solution (NaCl 0.9%) orally and injected with carrageenan (Inflammation control group); Group III: rats were administered hesperidin only (Hesperidin group); Group IV: rats were administered hesperidin orally and intrapleurally injected with 2% carrageenan (Inflammation treated with hesperidin group). The exudate volume, total leukocyte count, reactive oxygen species (ROS), myeloperoxidase (MPO),δ-aminolevulinate dehydratase (δ-ALA-D), catalase (CAT), superoxide dismutase (SOD), activities as well as non-protein thiol group (NPSH) and thiobarbituric acid reactive substances (TBARS) levels were determined. Pretreatment with hesperidin at a dose of 80 mg/kg orally per da...

Food research international (Ottawa, Ont.), 2018

Tiger nut tubers have been reportedly used for the treatment of erectile dysfunction (ED) in folk... more Tiger nut tubers have been reportedly used for the treatment of erectile dysfunction (ED) in folk medicine without scientific basis. Hence, this study evaluated the effect of tiger nut on erectile dysfunction by assessing biochemical parameters relevant to ED in male rats by nitric oxide synthase (NOS) inhibitor, Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME) treatment. Rats were divided into five groups (n = 10) each: Control group; l-NAME plus basal diet; l-NAME plus Sildenafil citrate; diet supplemented processed tiger nut (20%) plus l-NAME;diet supplemented raw tiger nut (20%) plus l-NAME. l-NAME pre-treatment (40 mg/kg/day) lasted for 14 days. Arginase, acetycholinesterase (AChE) and adenosine deaminase (ADA) activities as well as nitric oxide levels (NO) in serum, brain and penile tissue were measured. l-NAME increased the activity of arginase, AChE and ADA and reduced NO levels. However, dietary supplementation with tiger nut caused a reduction on the activities of t...

Journal of Functional Foods

The International Journal of Biotechnology, 2016

Male sexual dysfunction (MSD) might be produced by multifactorial determinants which include psyc... more Male sexual dysfunction (MSD) might be produced by multifactorial determinants which include psychological disorders, androgen deficiencies, chronic medical conditions, vascular insufficiency, penile disease, pelvic surgery, neurological disorders, drugs, life style, aging and systemic diseases. This study sought to assess the antioxidant properties of the water-extractable component of the leaves of Black plum (Vitex doniana) and bark of Kola nut (Cola nitida),evaluate their effect on pro-oxidant generated lipid peroxidation in rat's testes; and examine the effect on arginase which is among the major enzymes associated with Erectile dysfunction. The results of the total phenol, total flavonoid of aqueous extracts of Cola nitida and Vitex doniana revealed that Cola nitida (10.64 mgGAE/g) had significantly (P<0.05) more total phenol content than Vitex doniana (4.68 mgGAE/g). The result also revealed that Vitex doniana (2.1 mgQE/g) had significantly (P<0.05) higher total flavonoid content than Cola nitida (1.3 mgQE/g). Also, Vitex doniana (20.24 mgAEE/g) had significantly (p<0.05) higher reducing property than Cola nitida (17.43 mgAEE/g). The results of the 2, 2'-azino-bis (3-ethylbenthiazoline-6-sulphonic acid (ABTS *) radical mopping up capacity of the water extractable component of Vitex doniana and Cola nitida also showed that the extracts are able to scavenge ABTS* radicals, however, Vitex doniana (1.8 Mmol TEAC/100g) had significantly (P<0.05) higher ABTS* mopping up capability than Cola nitida (1.2 Mmol TEAC/100g). Furthermore, the DPPH* result revealed that Cola nitida and Vitex doniana extracts scavenged DPPH* radicals in a concentration-dependent pattern. However, Vitex doniana (IC50 = 1.28mg/ml) had a significantly (P<0.05) higher DPPH* mopping up capability than Cola nitida (IC50 = 0.83 mg/ml). Both extracts were able to inhibit FeSO4-generated lipid peroxidation at a dose-dependent manner; however, Vitex doniana (IC50 = 1.07 mg/ml) had a higher inhibitory action of Fe 2+ induced lipid peroxidation than Cola nitida (IC50 = 1.01 mg/ml). Vitex doniana (IC50 = 0.38mg/ml) has the higher arginase inhibitory activity than Cola nitida (IC50 = 0.34mg/ml). High phenolic content and strong antioxidant properties could be part of the mechanisms through which the water extractable phytochemicals of Cola nitida (bark) and Vitex doniana (leaves) exhibits its preventive measure of erectile function. However, Vitex doniana displayed a stronger effect on Male reproductive function than Cola nitida.

Journal of Food Biochemistry, 2016

Phytomedicine : international journal of phytotherapy and phytopharmacology, 2018

Tiger nut (Cyperus esculentus L.) and walnut (Tetracarpidium conophorum Müll. Arg.) have been rep... more Tiger nut (Cyperus esculentus L.) and walnut (Tetracarpidium conophorum Müll. Arg.) have been reportedly used in the treatment of inflammatory diseases such as atherosclerosis, prevent heart attack and improve blood circulation, reduce serum cholesterol level as well as inhibit oxidation reactions. This study investigated the effect of tiger nut and walnut hydro-alcoholic extracts on extracellular metabolism of ATP through the NOS/cGMP/PKG signaling pathway induced by Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) in kidney slices. The plants were extracted for 24 h in 10 ml of 70% ethanol and 30% distilled water per gram milled material on a mechanical shaker and filtered using Whatman filter paper. The effect of the extracts on ecto-nucleotidases (NTPDase and 5' nucleotidase) and adenosine deaminase activities, nitrites and nitrates levels (NO, markers of NO production) as well as lipid and protein oxidation reactions in kidney slices were evaluated. Also, the phenoli...