BABBIKER GORISH - Academia.edu (original) (raw)

Papers by BABBIKER GORISH

F1000Research

Background: The complement system is made up of an abundance of unique plasma proteins that play ... more Background: The complement system is made up of an abundance of unique plasma proteins that play an important role in innate immunity and inflammation, aiding in the fight against pathogenic microbes and viral diseases. The purpose of this study was to evaluate the serum complement C4 concentration in COVID-19 patients in Khartoum and compare them to healthy controls. Methods: A total of 100 samples were collected, 50 samples from COVID-19 patients who presented as cases and 50 samples from people who were evidently healthy. Overall, 33 (66%) the patient populations in the case group were not in the hospital’s intensive care unit (ICU), compared to 17 (34%) who were. The concentrations of C4 in each serum sample were calculated in milligrams per deciliter. SPSS version (20) was used to analyze the data. Results: The means level of complement C4 (mg/dL) were 37.44±18.618, 23.90±10.229 in the case group and in the control group, respectively. There was a statistically significant diff...

Asian Journal of Research in Biochemistry

Background: Diabetes mellitus (DM) is a systemic metabolic disorder that can lead to diabetic nep... more Background: Diabetes mellitus (DM) is a systemic metabolic disorder that can lead to diabetic nephropathy (DN), a leading cause of end-stage renal disease around the world. Objectives: The purpose of this study was to compare microalbuminuria and glycosylated hemoglobin (HbA1c) levels in patients with diabetes mellitus to healthy normal controls in Shendi, Sudan. Materials and Methods: This hospital-based case-control study was carried out in Shendi, Sudan. Between June and October of 2021 This study included 50 participants, 30 of whom had clinically confirmed diabetes as cases and 20 who appeared to be healthy as controls. In this study, blood and random urine samples were collected from each case and control, and the levels of microalbuminuria and hemoglobin A1C (HbA1C) were estimated using an A25 chemistry analyzer and an Ichroma immuno-analyzer, respectively. The data was collected with a structured questionnaire and analyzed with SPSS version 20. Results: In our study, patient...

Asian Journal of Research in Biochemistry

Insulin resistance (IR) is an essential factor in the etiology of type2 diabetes and is likewise ... more Insulin resistance (IR) is an essential factor in the etiology of type2 diabetes and is likewise related to a big range of different pathophysiologic sequelae along with hypertension, hyperlipidemia. we carried out this cross-sectional examination to the evaluation of insulin resistance in obese people. A total of 60 obese people 20% males and 80% female were divided into 4 groups overweight (20.0%), obesity class1(36.6%), obesity class2(16.6%), and obesity class3(26.6%). Blood samples were obtained from each participant and analyzed for fasting blood glucose (FBG) as well as insulin levels. Data were analyzed by using SPSS version 20. The results showed a positive correlation between insulin resistance and insulin levels (mean 1.1+0.91, 7.1+10.1, r=0.490, P. value= 0.0001 respectively). There was no correlation between insulin resistance and FBG (mean 1.1+0.91, 75.7+10.9, r=0.52, P. value = 0.691 respectively). The analysis also showed a significant positive correlation between ins...

BMC Public Health

Background One of the four main elements of the worldwide polio eradication strategy is acute fla... more Background One of the four main elements of the worldwide polio eradication strategy is acute flaccid paralysis surveillance (AFP). This system is based on (acute flaccid paralysis (AFP) cases reported and tested at World Health Organization (WHO) accredited laboratories. To measure and monitor performance, indicators were created. The current study aims to evaluate the system components, performance, and efficiency in River Nile State, Northern Sudan, and their compliance with World Health Organization (WHO) requirements for it to be adopted as a good system; its results can be used to certify whether a country is polio-free or not. Material and methods A facility-based retrospective descriptive study was conducted in the River Nile State, Northern Sudan, from Jan 2017 to Dec 2020. This study included all reporting sites/units, workers who reported acute flaccid paralysis (AFP) cases, and officers at the locality level. A total of 50 health institutions were visited for surveillanc...

Cancer Control

Background In vitro studies have produced conflicting results about the significance of the JC Po... more Background In vitro studies have produced conflicting results about the significance of the JC Polyoma Virus (JCV) in the human cancers. Objectives Our study aims to detect the presence of JCV Large T antigen (LTag) together with viral load quantitation in the prostate tumor samples to assess if JCV harbors risk factor for prostate cancer (PCa). Method This was a case control-based study. A total of 110 patients participated in this study, including 55 patients with PCa and another 55 patients with benign prostatic hyperplasia (BPH) as cases and controls, respectively. Tissue, blood and urine samples were collected from each participant. Tissues samples were analyzed for the presence of JCV Ltag using a direct immunofluorescence assay (IF). Only positive IF tested samples were subjected to viral quantitation assay. Data were collected and managed using SPSS version 20. Result The JCV LTag in the cases group was 23.63% (13/55) which was higher than that of the controls group 5.45% (3...

Microbiology Research Journal International

Background: The prevalence of nosocomial infections has continued to rise due to microbial contam... more Background: The prevalence of nosocomial infections has continued to rise due to microbial contamination of the hospital environment. Operating room contamination, on the other hand, is one of the most common and life-threatening sources of nosocomial infections. Objective: The goal of this research is to isolate and identify bacterial contaminants in operating rooms and equipment in EnNahud, West Kordofan. Methods: A total of 45 samples (from three hospitals) were collected from various operating theatre sites between September and December 2020. Using the accepted bacteriological methods (ISO/TC 147/SC 4 Microbiological techniques), all isolated bacteria were identified. S. Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 were employed as control bacterial strains to track the entire bacteriological process. Results: Five kinds of bacteria were recovered from the 45 (100%) positive specimens in three hospitals in this study. The number of polluted hospitals is on t...

International Journal of Pathogen Research

Background: Contact lenses have been widely used as an alternative to spectacles both in develope... more Background: Contact lenses have been widely used as an alternative to spectacles both in developed and non-developing countries. However, under certain circumstances, adverse responses can occur during contact lens wear and several microorganisms including bacteria, fungi, and free living amoebae—can cause several eye infections. This study was aimed to isolate contaminating bacteria from eye lenses solution. Methods: The samples were collected from solution bottles of eye lenses with the help of sterile cotton tipped swabs that were pre- moistened with sterile normal saline, then sample directly cultured on solid media. All samples were inoculated on to blood agar, MacConkey’s agar were incubated at 37oC for 24-48 h, Cultures were considered negative if no growth was detected within 48 hours of incubation. Bacterial culture obtained was identified using Gram’s staining, on the basis of culture diagnosis by growing on media and performing biochemical test. Results: A total of 150 sa...

International Journal of Pathogen Research

Background: Bacterial resistance is a more serious threat, and herbal medicines have been used as... more Background: Bacterial resistance is a more serious threat, and herbal medicines have been used as an alternative solution to this problem. Honey has been used to treat bacterial infections for decades. Methods: This is a prospective cross-sectional study conducted at the Microbiology Laboratory, Faculty of Laboratory Medicine, Shendi, Sudan between October 2021 and November 2021. In this study, 50 different clinical samples were collected from different microbiology laboratories in Shendi City, and different Gram-positive cocci bacteria were isolated and identified by Gram’s staining and biochemical tests. The susceptibility of Gram-positive bacteria to the bee honey was determined using agar well diffusion technique. Results: Out of a total of 50 clinical specimens cultured the most frequent isolated bacteria were Staphylococcus aureus (46%), Streptococcus epidermidis (36%), Streptococcus viridian's (10%), Staphylococcus saprophyticus (4%), while the least isolated bacteria was...

一般社団法人電子情報通信学会, Jan 16, 2013

South Asian Journal of Research in Microbiology, 2021

Objectives: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acq... more Objectives: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acquired resistance to many antibiotics, resulting in high morbidity and mortality. This study aimed to detect MDR Acinetobacter baumannii and its resistant genes (blaNDM, blaOXA48) from clinical isolates in Khartoum state. Methodology: A cross-sectional hospital-based study was conducted during the period fromApril to July 2019. A total of 50 clinical isolates were obtained from samples of patients in intensive care units (ICUs) for the purpose of molecular confirming of A. baumannii and detecting NDM and OXA-48 resistance genes by using conventional PCR. Results: Of the 50 isolates examined, 47 (94%) were confirmed to be A. baumannii, and 3 (6%) were other species. Moreover, the 47 A. baumannii isolates were furtherly examined for the presences of resistant genes and the result showed that NDM gene was detected in 2 isolates (4.3%) and OXA-48 gene was detected in only one isolate (2.1%). ...

Journal of Cancer Research Reviews & Reports, 2019

Patients with cancer are particularly under risk of many microbial infections and even septicaemi... more Patients with cancer are particularly under risk of many microbial infections and even septicaemia due to the weakened immune system induced by Chemotherapy. This study was done to identify and isolate aerobic bacterial septicaemic pathogens among cancer patients. This study was performed in Radiation and Isotopes national Centre of Khartoum Hospital (previously Alzarra) during the period from September to November 2019. One Hundred Fifty blood samples were collected from cancer patients suspected to have septicemia. All samples where inculated in blood culture media and incubated aerobically. Upon detection of growth signs the bacterial isolates were subcultured and identified according to the Standard known procedure. Antimicrobial sensitivity test was done following CLSI guidelines, The study showed that Twenty two (14%) of the investigated samples were showed growth signs, while One hundred Twenty eight (86%) were showed no growth. After subculture on Blood agar, MacConky agar a...

A dissertation submitted in partial fulfillment for the requirements of MSc in Medical Laboratory... more A dissertation submitted in partial fulfillment for the requirements of MSc in Medical Laboratory Science (Microbiology)

Journal of Vaccines & Vaccination, 2018

Infectious Agents and Cancer, 2019

Background Polyomavirus hominis1, also called BK virus (BKV) is a well-known etiological agent of... more Background Polyomavirus hominis1, also called BK virus (BKV) is a well-known etiological agent of renal transplant nephropathy and cystitis. Recently, it got great attention from the researcher as a principal predisposing factor for different kinds of cancers including prostate cancer (PCa). Thus, this study aims to determine the correlation between BKV infection and PCa through a descriptive case-control based study. Methods A total of 55 paraffin-embedded tissue blocks of patients with PCa and another 55 tissue blocks from BPH patients were obtained. In parallel, respective urine samples were collected from all the cases and controls. The existence of BKV large T antigen (LTAg) was analyzed by Direct Immunofluorescence assay. Only BKV LTAg positive specimens were further analyzed for the presence of viral DNA by using a conventional PCR then subjected to viral load quantitation by using Q-PCR. Result BKV LTAg was identified in 30% (17/55) of cases tissue specimens and only in 7% (...

Journal of Carcinogenesis & Mutagenesis, 2018

مجلة أداء المؤسسات الجزائرية, 2014

African Journal of Clinical and Experimental Microbiology, 2010

Журнал неорганической химии, 2014

Journal of Medical Virology, 2011

F1000Research

Background: The complement system is made up of an abundance of unique plasma proteins that play ... more Background: The complement system is made up of an abundance of unique plasma proteins that play an important role in innate immunity and inflammation, aiding in the fight against pathogenic microbes and viral diseases. The purpose of this study was to evaluate the serum complement C4 concentration in COVID-19 patients in Khartoum and compare them to healthy controls. Methods: A total of 100 samples were collected, 50 samples from COVID-19 patients who presented as cases and 50 samples from people who were evidently healthy. Overall, 33 (66%) the patient populations in the case group were not in the hospital’s intensive care unit (ICU), compared to 17 (34%) who were. The concentrations of C4 in each serum sample were calculated in milligrams per deciliter. SPSS version (20) was used to analyze the data. Results: The means level of complement C4 (mg/dL) were 37.44±18.618, 23.90±10.229 in the case group and in the control group, respectively. There was a statistically significant diff...

Asian Journal of Research in Biochemistry

Background: Diabetes mellitus (DM) is a systemic metabolic disorder that can lead to diabetic nep... more Background: Diabetes mellitus (DM) is a systemic metabolic disorder that can lead to diabetic nephropathy (DN), a leading cause of end-stage renal disease around the world. Objectives: The purpose of this study was to compare microalbuminuria and glycosylated hemoglobin (HbA1c) levels in patients with diabetes mellitus to healthy normal controls in Shendi, Sudan. Materials and Methods: This hospital-based case-control study was carried out in Shendi, Sudan. Between June and October of 2021 This study included 50 participants, 30 of whom had clinically confirmed diabetes as cases and 20 who appeared to be healthy as controls. In this study, blood and random urine samples were collected from each case and control, and the levels of microalbuminuria and hemoglobin A1C (HbA1C) were estimated using an A25 chemistry analyzer and an Ichroma immuno-analyzer, respectively. The data was collected with a structured questionnaire and analyzed with SPSS version 20. Results: In our study, patient...

Asian Journal of Research in Biochemistry

Insulin resistance (IR) is an essential factor in the etiology of type2 diabetes and is likewise ... more Insulin resistance (IR) is an essential factor in the etiology of type2 diabetes and is likewise related to a big range of different pathophysiologic sequelae along with hypertension, hyperlipidemia. we carried out this cross-sectional examination to the evaluation of insulin resistance in obese people. A total of 60 obese people 20% males and 80% female were divided into 4 groups overweight (20.0%), obesity class1(36.6%), obesity class2(16.6%), and obesity class3(26.6%). Blood samples were obtained from each participant and analyzed for fasting blood glucose (FBG) as well as insulin levels. Data were analyzed by using SPSS version 20. The results showed a positive correlation between insulin resistance and insulin levels (mean 1.1+0.91, 7.1+10.1, r=0.490, P. value= 0.0001 respectively). There was no correlation between insulin resistance and FBG (mean 1.1+0.91, 75.7+10.9, r=0.52, P. value = 0.691 respectively). The analysis also showed a significant positive correlation between ins...

BMC Public Health

Background One of the four main elements of the worldwide polio eradication strategy is acute fla... more Background One of the four main elements of the worldwide polio eradication strategy is acute flaccid paralysis surveillance (AFP). This system is based on (acute flaccid paralysis (AFP) cases reported and tested at World Health Organization (WHO) accredited laboratories. To measure and monitor performance, indicators were created. The current study aims to evaluate the system components, performance, and efficiency in River Nile State, Northern Sudan, and their compliance with World Health Organization (WHO) requirements for it to be adopted as a good system; its results can be used to certify whether a country is polio-free or not. Material and methods A facility-based retrospective descriptive study was conducted in the River Nile State, Northern Sudan, from Jan 2017 to Dec 2020. This study included all reporting sites/units, workers who reported acute flaccid paralysis (AFP) cases, and officers at the locality level. A total of 50 health institutions were visited for surveillanc...

Cancer Control

Background In vitro studies have produced conflicting results about the significance of the JC Po... more Background In vitro studies have produced conflicting results about the significance of the JC Polyoma Virus (JCV) in the human cancers. Objectives Our study aims to detect the presence of JCV Large T antigen (LTag) together with viral load quantitation in the prostate tumor samples to assess if JCV harbors risk factor for prostate cancer (PCa). Method This was a case control-based study. A total of 110 patients participated in this study, including 55 patients with PCa and another 55 patients with benign prostatic hyperplasia (BPH) as cases and controls, respectively. Tissue, blood and urine samples were collected from each participant. Tissues samples were analyzed for the presence of JCV Ltag using a direct immunofluorescence assay (IF). Only positive IF tested samples were subjected to viral quantitation assay. Data were collected and managed using SPSS version 20. Result The JCV LTag in the cases group was 23.63% (13/55) which was higher than that of the controls group 5.45% (3...

Microbiology Research Journal International

Background: The prevalence of nosocomial infections has continued to rise due to microbial contam... more Background: The prevalence of nosocomial infections has continued to rise due to microbial contamination of the hospital environment. Operating room contamination, on the other hand, is one of the most common and life-threatening sources of nosocomial infections. Objective: The goal of this research is to isolate and identify bacterial contaminants in operating rooms and equipment in EnNahud, West Kordofan. Methods: A total of 45 samples (from three hospitals) were collected from various operating theatre sites between September and December 2020. Using the accepted bacteriological methods (ISO/TC 147/SC 4 Microbiological techniques), all isolated bacteria were identified. S. Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 were employed as control bacterial strains to track the entire bacteriological process. Results: Five kinds of bacteria were recovered from the 45 (100%) positive specimens in three hospitals in this study. The number of polluted hospitals is on t...

International Journal of Pathogen Research

Background: Contact lenses have been widely used as an alternative to spectacles both in develope... more Background: Contact lenses have been widely used as an alternative to spectacles both in developed and non-developing countries. However, under certain circumstances, adverse responses can occur during contact lens wear and several microorganisms including bacteria, fungi, and free living amoebae—can cause several eye infections. This study was aimed to isolate contaminating bacteria from eye lenses solution. Methods: The samples were collected from solution bottles of eye lenses with the help of sterile cotton tipped swabs that were pre- moistened with sterile normal saline, then sample directly cultured on solid media. All samples were inoculated on to blood agar, MacConkey’s agar were incubated at 37oC for 24-48 h, Cultures were considered negative if no growth was detected within 48 hours of incubation. Bacterial culture obtained was identified using Gram’s staining, on the basis of culture diagnosis by growing on media and performing biochemical test. Results: A total of 150 sa...

International Journal of Pathogen Research

Background: Bacterial resistance is a more serious threat, and herbal medicines have been used as... more Background: Bacterial resistance is a more serious threat, and herbal medicines have been used as an alternative solution to this problem. Honey has been used to treat bacterial infections for decades. Methods: This is a prospective cross-sectional study conducted at the Microbiology Laboratory, Faculty of Laboratory Medicine, Shendi, Sudan between October 2021 and November 2021. In this study, 50 different clinical samples were collected from different microbiology laboratories in Shendi City, and different Gram-positive cocci bacteria were isolated and identified by Gram’s staining and biochemical tests. The susceptibility of Gram-positive bacteria to the bee honey was determined using agar well diffusion technique. Results: Out of a total of 50 clinical specimens cultured the most frequent isolated bacteria were Staphylococcus aureus (46%), Streptococcus epidermidis (36%), Streptococcus viridian's (10%), Staphylococcus saprophyticus (4%), while the least isolated bacteria was...

一般社団法人電子情報通信学会, Jan 16, 2013

South Asian Journal of Research in Microbiology, 2021

Objectives: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acq... more Objectives: Acinetobacter baumannii is an opportunistic bacterial pathogen with intrinsic and acquired resistance to many antibiotics, resulting in high morbidity and mortality. This study aimed to detect MDR Acinetobacter baumannii and its resistant genes (blaNDM, blaOXA48) from clinical isolates in Khartoum state. Methodology: A cross-sectional hospital-based study was conducted during the period fromApril to July 2019. A total of 50 clinical isolates were obtained from samples of patients in intensive care units (ICUs) for the purpose of molecular confirming of A. baumannii and detecting NDM and OXA-48 resistance genes by using conventional PCR. Results: Of the 50 isolates examined, 47 (94%) were confirmed to be A. baumannii, and 3 (6%) were other species. Moreover, the 47 A. baumannii isolates were furtherly examined for the presences of resistant genes and the result showed that NDM gene was detected in 2 isolates (4.3%) and OXA-48 gene was detected in only one isolate (2.1%). ...

Journal of Cancer Research Reviews & Reports, 2019

Patients with cancer are particularly under risk of many microbial infections and even septicaemi... more Patients with cancer are particularly under risk of many microbial infections and even septicaemia due to the weakened immune system induced by Chemotherapy. This study was done to identify and isolate aerobic bacterial septicaemic pathogens among cancer patients. This study was performed in Radiation and Isotopes national Centre of Khartoum Hospital (previously Alzarra) during the period from September to November 2019. One Hundred Fifty blood samples were collected from cancer patients suspected to have septicemia. All samples where inculated in blood culture media and incubated aerobically. Upon detection of growth signs the bacterial isolates were subcultured and identified according to the Standard known procedure. Antimicrobial sensitivity test was done following CLSI guidelines, The study showed that Twenty two (14%) of the investigated samples were showed growth signs, while One hundred Twenty eight (86%) were showed no growth. After subculture on Blood agar, MacConky agar a...

A dissertation submitted in partial fulfillment for the requirements of MSc in Medical Laboratory... more A dissertation submitted in partial fulfillment for the requirements of MSc in Medical Laboratory Science (Microbiology)

Journal of Vaccines & Vaccination, 2018

Infectious Agents and Cancer, 2019

Background Polyomavirus hominis1, also called BK virus (BKV) is a well-known etiological agent of... more Background Polyomavirus hominis1, also called BK virus (BKV) is a well-known etiological agent of renal transplant nephropathy and cystitis. Recently, it got great attention from the researcher as a principal predisposing factor for different kinds of cancers including prostate cancer (PCa). Thus, this study aims to determine the correlation between BKV infection and PCa through a descriptive case-control based study. Methods A total of 55 paraffin-embedded tissue blocks of patients with PCa and another 55 tissue blocks from BPH patients were obtained. In parallel, respective urine samples were collected from all the cases and controls. The existence of BKV large T antigen (LTAg) was analyzed by Direct Immunofluorescence assay. Only BKV LTAg positive specimens were further analyzed for the presence of viral DNA by using a conventional PCR then subjected to viral load quantitation by using Q-PCR. Result BKV LTAg was identified in 30% (17/55) of cases tissue specimens and only in 7% (...

Journal of Carcinogenesis & Mutagenesis, 2018

مجلة أداء المؤسسات الجزائرية, 2014

African Journal of Clinical and Experimental Microbiology, 2010

Журнал неорганической химии, 2014

Journal of Medical Virology, 2011