Bernard Broussin - Academia.edu (original) (raw)

Uploads

Papers by Bernard Broussin

Research paper thumbnail of FIVATE : le transfert impossible

Auteur(s) : Bernard Broussin Aquitaine Sante Medecine de la Reproduction, Ifreares Bordeaux, Poly... more Auteur(s) : Bernard Broussin Aquitaine Sante Medecine de la Reproduction, Ifreares Bordeaux, Polyclinique Jean Villar, 33520 Bruges La situation clinique de transfert impossible est une situation peu frequente. Dans un travail publie en 1998 sur notre experience de huit ans [1] nous avions denombre pour 3 667 transferts 1 % d’echecs. Beaucoup de travaux ont ete publies en vue de l’amelioration de la qualite du transfert mais il est tres difficile de connaitre dans la litterature [...]

Research paper thumbnail of In utero seizures revealing dentato-olivary dysplasia caused by SCN2A mutation

Neuropathology and Applied Neurobiology, 2017

Research paper thumbnail of Biochemical analysis of ascites fluid as an aid to etiological diagnosis: a series of 100 cases of nonimmune fetal ascites

Prenatal diagnosis, 2015

The aim of this study is to analyze the contribution of biochemistry and cytology of fetal ascite... more The aim of this study is to analyze the contribution of biochemistry and cytology of fetal ascites fluid to the etiological diagnosis of ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. This is a retrospective study of 100 consecutive cases of nonimmune fetal ascites in which ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal ascites were studied by biochemistry (total protein, β2 -microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). The etiology of ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P < 0.001) low levels of total protein in ascites of urinary origin, high levels of digestive enzymes in ascites of digestive origin, and high β2 -microglobulin in infectious ascites. Vacuolated cells were...

Research paper thumbnail of Prenatal diagnosis of atypical cystic lesions of the fetal scalp

Ultrasound in Obstetrics and Gynecology, 1998

Two cases of cystic lesions of the fetal scalp are described. They were initially thought to be m... more Two cases of cystic lesions of the fetal scalp are described. They were initially thought to be meningoceles, but the brain was normal on ultrasound examination and there were no associated abnormalities. In the first case, by 26 weeks&#39; gestation, the original cystic lesion had regressed to a dense tissue mass; in the second case, the cyst remained as a small anechoic mass. Postnatal assessment in the first case revealed a plexiform skin lesion measuring 40 x 15 mm on the left parietal bone. This was mobile and not attached to the underlying structures. The skull showed no obvious defect and a cranial scan was normal. In the second case, an epidermal cyst was diagnosed.

Research paper thumbnail of Contribution of a New PCR assay to the prenatal diagnosis of congenital toxoplasmosis

Prenatal Diagnosis, 1992

A polymerase chain reaction (PCR) assay has been developed for the detection of Toxoplasma gondii... more A polymerase chain reaction (PCR) assay has been developed for the detection of Toxoplasma gondii. The target sequence (88 bp) is part of a rDNA repetitive gene. A signal can be observed with only one parasite. It is directly and rapidly detected by electrophoresis and ethidium bromide staining. We report a prospective study of 80 documented cases of toxoplasmic seroconversions during pregnancy. The PCR assay of the amniotic fluids was compared with the current standard methods for diagnosis of fetal infection. Seventy specimens gave no PCR signal, and were negative according to prenatal tests and postnatal examinations. The presence of T. gondii was detected in ten specimens by PCR analysis. Four were confirmed by isolation of the parasite from the amniotic fluid; four by biological study of the fetal blood. For the remaining two, infection was diagnosed after birth. Together with ultrasonographic and biological data, this technique permits prenatal diagnosis within 1 day.

Research paper thumbnail of Fraser syndrome: Prenatal ultrasonic detection

American Journal of Medical Genetics, 1989

The report by P.A. Boyd et al. (19881 of 11 cases of Fraser syndrome prompts us to relate the fol... more The report by P.A. Boyd et al. (19881 of 11 cases of Fraser syndrome prompts us to relate the following case which was discovered by abnormal scan at 18 weeks of gestation. A 33-year-old GzPo healthy French woman,

Research paper thumbnail of Cell-free fetal DNA versus maternal serum screening for trisomy 21 in pregnant women with and without assisted reproduction technology: a prospective interventional study

Genetics in Medicine, 2018

Cell-free DNA (cfDNA) as a primary screening test has been available for years but few studies ha... more Cell-free DNA (cfDNA) as a primary screening test has been available for years but few studies have addressed this option in a prospective manner. The question is of interest after reports that maternal serum screening (MSS) is less accurate for pregnancies resulting from assisted reproduction technologies (ART) than for spontaneous pregnancies (SP). Methods: A prospective interventional study was designed to address the performances of cfDNA compared with MSS in pregnancies with or without ART. Each patient was offered both MSS and cfDNA testing. The primary analysis cohort ultimately included 794 patients with a spontaneous pregnancy (SP) (n = 472) or pregnancy obtained after ART (n = 322). Results: Overall, the false-positive rate and positive predictive value were 6.6% and 8.8% for MSS but 0% and 100% for cfDNA. MSS false-positive rate and positive predictive values were clearly poorer in the ART group (11.7% and 2.6%) than in the SP group (3.2% and 21.1%). The global rates of invasive procedures were 1.9% (15/794) with cfDNA but 8.4% (65/794) if MSS alone was proposed. Conclusion: cfDNA achieved better performance than MSS in both spontaneous and ART pregnancies, thus decreasing the number of invasive procedures. Our findings suggest that cfDNA should be considered for primary screening, especially in pregnancies obtained after ART.

Research paper thumbnail of FIVATE : le transfert impossible

Auteur(s) : Bernard Broussin Aquitaine Sante Medecine de la Reproduction, Ifreares Bordeaux, Poly... more Auteur(s) : Bernard Broussin Aquitaine Sante Medecine de la Reproduction, Ifreares Bordeaux, Polyclinique Jean Villar, 33520 Bruges La situation clinique de transfert impossible est une situation peu frequente. Dans un travail publie en 1998 sur notre experience de huit ans [1] nous avions denombre pour 3 667 transferts 1 % d’echecs. Beaucoup de travaux ont ete publies en vue de l’amelioration de la qualite du transfert mais il est tres difficile de connaitre dans la litterature [...]

Research paper thumbnail of In utero seizures revealing dentato-olivary dysplasia caused by SCN2A mutation

Neuropathology and Applied Neurobiology, 2017

Research paper thumbnail of Biochemical analysis of ascites fluid as an aid to etiological diagnosis: a series of 100 cases of nonimmune fetal ascites

Prenatal diagnosis, 2015

The aim of this study is to analyze the contribution of biochemistry and cytology of fetal ascite... more The aim of this study is to analyze the contribution of biochemistry and cytology of fetal ascites fluid to the etiological diagnosis of ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. This is a retrospective study of 100 consecutive cases of nonimmune fetal ascites in which ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal ascites were studied by biochemistry (total protein, β2 -microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). The etiology of ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P < 0.001) low levels of total protein in ascites of urinary origin, high levels of digestive enzymes in ascites of digestive origin, and high β2 -microglobulin in infectious ascites. Vacuolated cells were...

Research paper thumbnail of Prenatal diagnosis of atypical cystic lesions of the fetal scalp

Ultrasound in Obstetrics and Gynecology, 1998

Two cases of cystic lesions of the fetal scalp are described. They were initially thought to be m... more Two cases of cystic lesions of the fetal scalp are described. They were initially thought to be meningoceles, but the brain was normal on ultrasound examination and there were no associated abnormalities. In the first case, by 26 weeks&#39; gestation, the original cystic lesion had regressed to a dense tissue mass; in the second case, the cyst remained as a small anechoic mass. Postnatal assessment in the first case revealed a plexiform skin lesion measuring 40 x 15 mm on the left parietal bone. This was mobile and not attached to the underlying structures. The skull showed no obvious defect and a cranial scan was normal. In the second case, an epidermal cyst was diagnosed.

Research paper thumbnail of Contribution of a New PCR assay to the prenatal diagnosis of congenital toxoplasmosis

Prenatal Diagnosis, 1992

A polymerase chain reaction (PCR) assay has been developed for the detection of Toxoplasma gondii... more A polymerase chain reaction (PCR) assay has been developed for the detection of Toxoplasma gondii. The target sequence (88 bp) is part of a rDNA repetitive gene. A signal can be observed with only one parasite. It is directly and rapidly detected by electrophoresis and ethidium bromide staining. We report a prospective study of 80 documented cases of toxoplasmic seroconversions during pregnancy. The PCR assay of the amniotic fluids was compared with the current standard methods for diagnosis of fetal infection. Seventy specimens gave no PCR signal, and were negative according to prenatal tests and postnatal examinations. The presence of T. gondii was detected in ten specimens by PCR analysis. Four were confirmed by isolation of the parasite from the amniotic fluid; four by biological study of the fetal blood. For the remaining two, infection was diagnosed after birth. Together with ultrasonographic and biological data, this technique permits prenatal diagnosis within 1 day.

Research paper thumbnail of Fraser syndrome: Prenatal ultrasonic detection

American Journal of Medical Genetics, 1989

The report by P.A. Boyd et al. (19881 of 11 cases of Fraser syndrome prompts us to relate the fol... more The report by P.A. Boyd et al. (19881 of 11 cases of Fraser syndrome prompts us to relate the following case which was discovered by abnormal scan at 18 weeks of gestation. A 33-year-old GzPo healthy French woman,

Research paper thumbnail of Cell-free fetal DNA versus maternal serum screening for trisomy 21 in pregnant women with and without assisted reproduction technology: a prospective interventional study

Genetics in Medicine, 2018

Cell-free DNA (cfDNA) as a primary screening test has been available for years but few studies ha... more Cell-free DNA (cfDNA) as a primary screening test has been available for years but few studies have addressed this option in a prospective manner. The question is of interest after reports that maternal serum screening (MSS) is less accurate for pregnancies resulting from assisted reproduction technologies (ART) than for spontaneous pregnancies (SP). Methods: A prospective interventional study was designed to address the performances of cfDNA compared with MSS in pregnancies with or without ART. Each patient was offered both MSS and cfDNA testing. The primary analysis cohort ultimately included 794 patients with a spontaneous pregnancy (SP) (n = 472) or pregnancy obtained after ART (n = 322). Results: Overall, the false-positive rate and positive predictive value were 6.6% and 8.8% for MSS but 0% and 100% for cfDNA. MSS false-positive rate and positive predictive values were clearly poorer in the ART group (11.7% and 2.6%) than in the SP group (3.2% and 21.1%). The global rates of invasive procedures were 1.9% (15/794) with cfDNA but 8.4% (65/794) if MSS alone was proposed. Conclusion: cfDNA achieved better performance than MSS in both spontaneous and ART pregnancies, thus decreasing the number of invasive procedures. Our findings suggest that cfDNA should be considered for primary screening, especially in pregnancies obtained after ART.