BEYHAN BUDAK - Academia.edu (original) (raw)

Papers by BEYHAN BUDAK

Diagnostic microbiology and infectious disease, Jul 1, 2024

Human Vaccines & Immunotherapeutics, Nov 14, 2023

Infectious diseases and clinical microbiology, Jun 13, 2022

We aimed to describe the antibody response against SARS-CoV-2 after inactivated COVID-19 vaccine ... more We aimed to describe the antibody response against SARS-CoV-2 after inactivated COVID-19 vaccine in elderly individuals. SARS-CoV-2 IgG levels were measured in the blood samples of 126 volunteers over the age of 60. The antibody positivity rate was 42.8% after the first dose and 96.8% after the second dose of the vaccine. The median antibody titers after two vaccine doses were 561.3AU/mL and 43AU/mL, respectively(p<0.001). After vaccination, 22.2% of the participants had antibodies equivalent to 1:80 dilutions in plaque reduction neutralization test (PNRT). We believe that the booster dose is needed to continue the protective immune response in especially elderly groups.

Vaccine, Apr 1, 2022

Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against... more Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against COVID-19 disease. This study aimed to assess the association between the vaccination with CoronaVac and the COVID-19 infections among the health care workers in a university hospital and to determine the vaccine effectiveness against COVID-19 in a period when alpha variant was dominant. Methods This retrospective cohort study was conducted in a university hospital in Istanbul, Turkey employs 4067 health care workers. The follow-up period was defined as starting 14 days after receiving the second dose for fully vaccinated group. Health care workers were censored when have a positive PCR test result or at the end of the study. Unvaccinated health care workers were censored if they receive any COVID-19 vaccine doses. The incidence rate ratio and Cox regression were used to estimate the unadjusted and adjusted effectiveness of the vaccine. Findings: Seventy-one percent of the health care workers were fully vaccinated whereas 29% percent did not receive any doses. The incidence rate of SARS-CoV-2 infection was 133.7 vs 70.7 per 100.000 person-days in the unvaccinated and fully vaccinated groups, respectively. The unadjusted effectiveness against COVID-19 infection was 47% (95% CI 31–59%) whereas adjusted effectiveness was 39% (95% CI 20–64%). Interpretation: This real life study conducted in health care workers demonstrated that the effectiveness of two doses of the CoronaVac vaccine (39%) was lower than that determined in clinical trials. Due to reduce in protection over time or against variants, booster doses may be needed.

Sağlık bilimleri ve meslekleri dergisi /journal of health sciences of professions, Oct 14, 2018

Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve... more Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve umutsuzluk düzeyine etkisini belirlemek amacıyla planlanmıştır. Gereç ve Yöntem: Çalışmanın yapıldığı üniversite hastanesinin gastroenteroloji polikliniğine IBS tanısı almış günde ortalama 3 hasta başvurmaktadır. Araştırmanın evrenini 01.03.2017-05.08.2017 tarihleri arasında polikliniğe başvuran 314 hasta, örneklemini ise 18 yaş ve üzeri, IBS tanısı almış, iletişim sorunu olmayan ve araştırmaya katılmayı kabul eden 209 birey oluşturmuştur. Veriler hastaların sosyodemografik özelliklerini içeren Hasta Tanıtım Formu, Gastrointestinal Semptom Değerlendirme Ölçeği(GSDÖ, Beck Depresyon Ölçeği(BDÖ) ve Beck Umutsuzluk Ölçeği(BUÖ) ile toplanmıştır. Verilerin değerlendirilmesinde yüzdelik, t testi, kikare testi, ANOVA ve pearson korelasyon testi kullanılmıştır. Bulgular: Yaş ortalaması 40.69±14.0 olan hastaların, ortalama hastalık süresi 6.0±1.3 yıldır. Hastaların GSDÖ'nden aldıkları toplam puan ortalaması 52.0±1.65 olup, orta düzeyde semptom şiddetine sahip oldukları görülmüştür.. BUÖ puan ortalaması 8.0±3.1, BDS puan ortalaması 10.0±6.9 olup, hafif düzeyde umutsuzluk ve depresyon belirtisine sahip olduğu görülmüştür. GSDÖ alt boyutlarından karın ağrısı, reflü, hazımsızlık, diyare ve GSDÖ toplam puanıyla BUÖ ve BDÖ arasında anlamlı bir ilişki saptanmazken, konstipasyon alt boyut puanıyla BUÖ arasında pozitif yönde orta düzeyde bir ilişki saptanmıştır. Benzer şekilde BDÖ ile konstipasyon alt boyut puanıyla pozitif yönde güçlü düzeyde bir ilişki saptanmıştır. Sonuç: Konstipasyon ile depresyon ve umutsuzluk arasında güçlü ve orta düzeyde bir ilişki saptanmıştır. Özellikle konstipasyonu olan hastaların depresyon ve umutsuzluk düzeyi açısından değerlendirilmesi gerekmektedir.

Diagnostic Microbiology and Infectious Disease, Oct 1, 2022

The objective of our study was to evaluate the antibody responses of health care workers (HCWs) w... more The objective of our study was to evaluate the antibody responses of health care workers (HCWs) who were vaccinated with booster dose BNT162b2 6 months after 2 doses of the CoronoVac vaccine. The study included 318 HCWs vaccinated with inactive CoronaVac SARS-CoV-2 vaccine in 2 doses. Anti-spike/RBD IgG levels were measured immediately before and 1 month after the booster dose. In the sixth month after CoronaVac vaccination, the median of antibody levels of 1212.02 AU/ML, while it was 9283 AU/mL after BNT162b2 vaccination. IgG antibody titers of over 1050 AU/mL (which is equivalent to 1:80 dilution in the plaque reduction neutralization test) were detected in HCWs 15.09% and 97.8%, respectively. Our results showed that antibody titers increased 8-fold after the booster dose. We believe that the administration of the mRNA vaccine as a booster dose can provide more effective protection against COVID-19 infection, especially in individuals with risk factors.

Irish Journal of Medical Science, Mar 28, 2022

Aims Despite high vaccination rates, increasing case numbers continue to be reported with the ide... more Aims Despite high vaccination rates, increasing case numbers continue to be reported with the identification of new variants of concern, and the issue of durability of the vaccine-induced immune response remains hot topic. Real-life data regarding time-dependent immunogenicity of inactivated COVID-19 vaccines are scarce. We aimed to investigate the changes in the antibody at the different times after the second dose of the CoronaVac vaccine. Methods The study included 175 HCWs vaccinated with inactive CoronaVac (Sinovac Life Sciences, China) SARS-CoV-2 vaccine in two doses. Anti-spike/RBD IgG levels were measured first, third, and sixth months after the second dose. Chemiluminescent microparticle immunoassay (IgG II Quant test, Abbott, USA), which is 100% compatible with plaque reduction neutralization test, was used. Results Mean age of the participants was 38 ± 11.23 years (range between 22 and 66) of whom 119 (63.9%) were female, and 56 (32%) were male. Dramatic reductions were demonstrated in median antibody levels particularly in the infection-naïve group, comprising 138 HCWs compared to those with prior history of COVID-19 infection (n = 37) (p < 0.001). There was no difference between the two groups in terms of age, gender, blood groups, BMI, and comorbid diseases. Conclusions While antibody positivity remained above 90% in the 6th month after two doses of inactivated vaccine in HCWs, the median titers of neutralizing antibodies decreased rapidly. The decrease was more rapid and significant in those with no history of prior COVID-19 infection. In this critical phase of the pandemic, where we are facing the dominance of the Omicron variant after Delta, booster doses have become vital.

Cerrahpasa Medical Journal

Infectious Diseases and Clinical Microbiology

Vaccine, 2022

Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against... more Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against COVID-19 disease. This study aimed to assess the association between the vaccination with CoronaVac and the COVID-19 infections among the health care workers in a university hospital and to determine the vaccine effectiveness against COVID-19 in a period when alpha variant was dominant. Methods This retrospective cohort study was conducted in a university hospital in Istanbul, Turkey employs 4067 health care workers. The follow-up period was defined as starting 14 days after receiving the second dose for fully vaccinated group. Health care workers were censored when have a positive PCR test result or at the end of the study. Unvaccinated health care workers were censored if they receive any COVID-19 vaccine doses. The incidence rate ratio and Cox regression were used to estimate the unadjusted and adjusted effectiveness of the vaccine. Findings: Seventy-one percent of the health care workers were fully vaccinated whereas 29% percent did not receive any doses. The incidence rate of SARS-CoV-2 infection was 133.7 vs 70.7 per 100.000 person-days in the unvaccinated and fully vaccinated groups, respectively. The unadjusted effectiveness against COVID-19 infection was 47% (95% CI 31–59%) whereas adjusted effectiveness was 39% (95% CI 20–64%). Interpretation: This real life study conducted in health care workers demonstrated that the effectiveness of two doses of the CoronaVac vaccine (39%) was lower than that determined in clinical trials. Due to reduce in protection over time or against variants, booster doses may be needed.

Vaccine, 2021

Healthcare workers (HCWs) were among the first groups to be vaccinated in Turkey. The data to be ... more Healthcare workers (HCWs) were among the first groups to be vaccinated in Turkey. The data to be obtained by the vaccination of HCWs would guide wide spread vaccination programs. The study included 330 HCWs working at Istanbul University-Cerrahpaşa, Cerrahpaşa Medical Faculty Hospital and vaccinated with inactive CoronaVac (Sinovac Life Sciences, China) SARS-CoV-2 vaccine in two doses (28 days apart). Anti-Spike /RBD IgG levels were measured 14 days after the first dose and 28 days after the second dose. Chemiluminescent microparticle immunoassay (CMIA) (ARCHITECT IgG II Quant test, Abbott, USA), which is 100% compatible with plaque reduction neutralization test (PRNT), was used. Of the participants, 211 (63.9%) were female, 119 (36.1%) were male, and mean age was 39.6 ± 7.7 years. In those without prior COVID-19 history; (n = 255) antibody positivity was detected as 48.2% (95% CI: 42.1-54.3) 14 days after the first dose of vaccine, and 99.2% (95% CI: 98.1-100) at day 28 after the second dose. Antibody titers were significantly lower in patients with hypertension (p = 0.011). In those with prior history of COVID-19 (n = 75); both the antibody positivity rates after the first vaccine (48.2% vs 100%, p = 0.000) and the anti-spike/RBD antibody levels after the second vaccine (with a ≥ 1050 AU/mL titer equivalent to PRNT 1/80 dilution) was significant than infection-naive group (25.9% vs. 54.7%, p = 0.000). Antibody positivity after two doses of vaccination for all study group was 99.4% (95% CI: 98.6-100). Two doses CoronaVac produce effective humoral immunity in HCWs. Antibody response is significantly higher in those with prior history of COVID-19 than infection-naive group. Given no significant benefit of the second dose, a single shot of vaccination may be sufficient for those with prior history of COVID-19. Monitoring humoral and cellular immune responses, considering new variants, is required to validate this approach.

Vaccines, 2021

Healthcare workers are among risk groups in the COVID-19. Even if they are not infected with the ... more Healthcare workers are among risk groups in the COVID-19. Even if they are not infected with the disease, they witness the effects of the pandemic. The aim of the study is to determine the factors affecting COVID-19 vaccination status and reasons for vaccine hesitancy of healthcare personnel in our hospital. Firstly, the vaccination status and demographic characteristics of all healthcare personnel was evaluated. After that, a survey was applied to 408 vaccinated and 297 nonvaccinated personnel. Within the first month after the beginning of vaccination, 66% of 3937 healthcare personnel received a COVID-19 vaccine. The number of vaccinated personnel was higher among doctors, master graduates or higher educational levels and basic science-laboratory unit workers. In the surveyed group, being under the age of 50 (OR:1.85), being nondoctor healthcare personnel (nurse/midwife OR:1.78, administrative personnel OR:3.42, patient attendant/cleaning staff OR:4.11, security guard/other OR:2.96...

Sağlık Bilimleri ve Meslekleri Dergisi, 2018

Amac: Bu calisma irritable bagirsak sendromu olan hastalarda gastrointestinal semptomlarin depres... more Amac: Bu calisma irritable bagirsak sendromu olan hastalarda gastrointestinal semptomlarin depresyon ve umutsuzluk duzeyine etkisini belirlemek amaciyla planlanmistir. Gerec ve Yontem: Calismanin yapildigi universite hastanesinin gastroenteroloji poliklinigine IBS tanisi almis gunde ortalama 3 hasta basvurmaktadir. Arastirmanin evrenini 01.03.2017-05.08.2017 tarihleri arasinda poliklinige basvuran 314 hasta, orneklemini ise 18 yas ve uzeri, IBS tanisi almis, iletisim sorunu olmayan ve arastirmaya katilmayi kabul eden 209 birey olusturmustur. Veriler hastalarin sosyodemografik ozelliklerini iceren Hasta Tanitim Formu, Gastrointestinal Semptom Degerlendirme Olcegi(GSDO, Beck Depresyon Olcegi(BDO) ve Beck Umutsuzluk Olcegi(BUO) ile toplanmistir. Verilerin degerlendirilmesinde yuzdelik, t testi, kikare testi, ANOVA ve pearson korelasyon testi kullanilmistir. Bulgular: Y as ortalamasi 40.69±14.0 olan hastalarin, ortalama hastalik suresi 6.0±1.3 yildir. Hastalarin GSDO’nden aldiklari top...

Sağlık Bilimleri ve Meslekleri Dergisi, 2018

Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve... more Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve umutsuzluk düzeyine etkisini belirlemek amacıyla planlanmıştır. Gereç ve Yöntem: Çalışmanın yapıldığı üniversite hastanesinin gastroenteroloji polikliniğine IBS tanısı almış günde ortalama 3 hasta başvurmaktadır. Araştırmanın evrenini 01.03.2017-05.08.2017 tarihleri arasında polikliniğe başvuran 314 hasta, örneklemini ise 18 yaş ve üzeri, IBS tanısı almış, iletişim sorunu olmayan ve araştırmaya katılmayı kabul eden 209 birey oluşturmuştur. Veriler hastaların sosyodemografik özelliklerini içeren Hasta Tanıtım Formu, Gastrointestinal Semptom Değerlendirme Ölçeği(GSDÖ, Beck Depresyon Ölçeği(BDÖ) ve Beck Umutsuzluk Ölçeği(BUÖ) ile toplanmıştır. Verilerin değerlendirilmesinde yüzdelik, t testi, kikare testi, ANOVA ve pearson korelasyon testi kullanılmıştır. Bulgular: Yaş ortalaması 40.69±14.0 olan hastaların, ortalama hastalık süresi 6.0±1.3 yıldır. Hastaların GSDÖ'nden aldıkları toplam puan ortalaması 52.0±1.65 olup, orta düzeyde semptom şiddetine sahip oldukları görülmüştür.. BUÖ puan ortalaması 8.0±3.1, BDS puan ortalaması 10.0±6.9 olup, hafif düzeyde umutsuzluk ve depresyon belirtisine sahip olduğu görülmüştür. GSDÖ alt boyutlarından karın ağrısı, reflü, hazımsızlık, diyare ve GSDÖ toplam puanıyla BUÖ ve BDÖ arasında anlamlı bir ilişki saptanmazken, konstipasyon alt boyut puanıyla BUÖ arasında pozitif yönde orta düzeyde bir ilişki saptanmıştır. Benzer şekilde BDÖ ile konstipasyon alt boyut puanıyla pozitif yönde güçlü düzeyde bir ilişki saptanmıştır. Sonuç: Konstipasyon ile depresyon ve umutsuzluk arasında güçlü ve orta düzeyde bir ilişki saptanmıştır. Özellikle konstipasyonu olan hastaların depresyon ve umutsuzluk düzeyi açısından değerlendirilmesi gerekmektedir.

Irish Journal of Medical Science (1971 -), 2022

Aims Despite high vaccination rates, increasing case numbers continue to be reported with the ide... more Aims Despite high vaccination rates, increasing case numbers continue to be reported with the identification of new variants of concern, and the issue of durability of the vaccine-induced immune response remains hot topic. Real-life data regarding time-dependent immunogenicity of inactivated COVID-19 vaccines are scarce. We aimed to investigate the changes in the antibody at the different times after the second dose of the CoronaVac vaccine. Methods The study included 175 HCWs vaccinated with inactive CoronaVac (Sinovac Life Sciences, China) SARS-CoV-2 vaccine in two doses. Anti-spike/RBD IgG levels were measured first, third, and sixth months after the second dose. Chemiluminescent microparticle immunoassay (IgG II Quant test, Abbott, USA), which is 100% compatible with plaque reduction neutralization test, was used. Results Mean age of the participants was 38 ± 11.23 years (range between 22 and 66) of whom 119 (63.9%) were female, and 56 (32%) were male. Dramatic reductions were demonstrated in median antibody levels particularly in the infection-naïve group, comprising 138 HCWs compared to those with prior history of COVID-19 infection (n = 37) (p < 0.001). There was no difference between the two groups in terms of age, gender, blood groups, BMI, and comorbid diseases. Conclusions While antibody positivity remained above 90% in the 6th month after two doses of inactivated vaccine in HCWs, the median titers of neutralizing antibodies decreased rapidly. The decrease was more rapid and significant in those with no history of prior COVID-19 infection. In this critical phase of the pandemic, where we are facing the dominance of the Omicron variant after Delta, booster doses have become vital.

Diagnostic microbiology and infectious disease, Jul 1, 2024

Human Vaccines & Immunotherapeutics, Nov 14, 2023

Infectious diseases and clinical microbiology, Jun 13, 2022

We aimed to describe the antibody response against SARS-CoV-2 after inactivated COVID-19 vaccine ... more We aimed to describe the antibody response against SARS-CoV-2 after inactivated COVID-19 vaccine in elderly individuals. SARS-CoV-2 IgG levels were measured in the blood samples of 126 volunteers over the age of 60. The antibody positivity rate was 42.8% after the first dose and 96.8% after the second dose of the vaccine. The median antibody titers after two vaccine doses were 561.3AU/mL and 43AU/mL, respectively(p<0.001). After vaccination, 22.2% of the participants had antibodies equivalent to 1:80 dilutions in plaque reduction neutralization test (PNRT). We believe that the booster dose is needed to continue the protective immune response in especially elderly groups.

Vaccine, Apr 1, 2022

Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against... more Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against COVID-19 disease. This study aimed to assess the association between the vaccination with CoronaVac and the COVID-19 infections among the health care workers in a university hospital and to determine the vaccine effectiveness against COVID-19 in a period when alpha variant was dominant. Methods This retrospective cohort study was conducted in a university hospital in Istanbul, Turkey employs 4067 health care workers. The follow-up period was defined as starting 14 days after receiving the second dose for fully vaccinated group. Health care workers were censored when have a positive PCR test result or at the end of the study. Unvaccinated health care workers were censored if they receive any COVID-19 vaccine doses. The incidence rate ratio and Cox regression were used to estimate the unadjusted and adjusted effectiveness of the vaccine. Findings: Seventy-one percent of the health care workers were fully vaccinated whereas 29% percent did not receive any doses. The incidence rate of SARS-CoV-2 infection was 133.7 vs 70.7 per 100.000 person-days in the unvaccinated and fully vaccinated groups, respectively. The unadjusted effectiveness against COVID-19 infection was 47% (95% CI 31–59%) whereas adjusted effectiveness was 39% (95% CI 20–64%). Interpretation: This real life study conducted in health care workers demonstrated that the effectiveness of two doses of the CoronaVac vaccine (39%) was lower than that determined in clinical trials. Due to reduce in protection over time or against variants, booster doses may be needed.

Sağlık bilimleri ve meslekleri dergisi /journal of health sciences of professions, Oct 14, 2018

Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve... more Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve umutsuzluk düzeyine etkisini belirlemek amacıyla planlanmıştır. Gereç ve Yöntem: Çalışmanın yapıldığı üniversite hastanesinin gastroenteroloji polikliniğine IBS tanısı almış günde ortalama 3 hasta başvurmaktadır. Araştırmanın evrenini 01.03.2017-05.08.2017 tarihleri arasında polikliniğe başvuran 314 hasta, örneklemini ise 18 yaş ve üzeri, IBS tanısı almış, iletişim sorunu olmayan ve araştırmaya katılmayı kabul eden 209 birey oluşturmuştur. Veriler hastaların sosyodemografik özelliklerini içeren Hasta Tanıtım Formu, Gastrointestinal Semptom Değerlendirme Ölçeği(GSDÖ, Beck Depresyon Ölçeği(BDÖ) ve Beck Umutsuzluk Ölçeği(BUÖ) ile toplanmıştır. Verilerin değerlendirilmesinde yüzdelik, t testi, kikare testi, ANOVA ve pearson korelasyon testi kullanılmıştır. Bulgular: Yaş ortalaması 40.69±14.0 olan hastaların, ortalama hastalık süresi 6.0±1.3 yıldır. Hastaların GSDÖ'nden aldıkları toplam puan ortalaması 52.0±1.65 olup, orta düzeyde semptom şiddetine sahip oldukları görülmüştür.. BUÖ puan ortalaması 8.0±3.1, BDS puan ortalaması 10.0±6.9 olup, hafif düzeyde umutsuzluk ve depresyon belirtisine sahip olduğu görülmüştür. GSDÖ alt boyutlarından karın ağrısı, reflü, hazımsızlık, diyare ve GSDÖ toplam puanıyla BUÖ ve BDÖ arasında anlamlı bir ilişki saptanmazken, konstipasyon alt boyut puanıyla BUÖ arasında pozitif yönde orta düzeyde bir ilişki saptanmıştır. Benzer şekilde BDÖ ile konstipasyon alt boyut puanıyla pozitif yönde güçlü düzeyde bir ilişki saptanmıştır. Sonuç: Konstipasyon ile depresyon ve umutsuzluk arasında güçlü ve orta düzeyde bir ilişki saptanmıştır. Özellikle konstipasyonu olan hastaların depresyon ve umutsuzluk düzeyi açısından değerlendirilmesi gerekmektedir.

Diagnostic Microbiology and Infectious Disease, Oct 1, 2022

The objective of our study was to evaluate the antibody responses of health care workers (HCWs) w... more The objective of our study was to evaluate the antibody responses of health care workers (HCWs) who were vaccinated with booster dose BNT162b2 6 months after 2 doses of the CoronoVac vaccine. The study included 318 HCWs vaccinated with inactive CoronaVac SARS-CoV-2 vaccine in 2 doses. Anti-spike/RBD IgG levels were measured immediately before and 1 month after the booster dose. In the sixth month after CoronaVac vaccination, the median of antibody levels of 1212.02 AU/ML, while it was 9283 AU/mL after BNT162b2 vaccination. IgG antibody titers of over 1050 AU/mL (which is equivalent to 1:80 dilution in the plaque reduction neutralization test) were detected in HCWs 15.09% and 97.8%, respectively. Our results showed that antibody titers increased 8-fold after the booster dose. We believe that the administration of the mRNA vaccine as a booster dose can provide more effective protection against COVID-19 infection, especially in individuals with risk factors.

Irish Journal of Medical Science, Mar 28, 2022

Aims Despite high vaccination rates, increasing case numbers continue to be reported with the ide... more Aims Despite high vaccination rates, increasing case numbers continue to be reported with the identification of new variants of concern, and the issue of durability of the vaccine-induced immune response remains hot topic. Real-life data regarding time-dependent immunogenicity of inactivated COVID-19 vaccines are scarce. We aimed to investigate the changes in the antibody at the different times after the second dose of the CoronaVac vaccine. Methods The study included 175 HCWs vaccinated with inactive CoronaVac (Sinovac Life Sciences, China) SARS-CoV-2 vaccine in two doses. Anti-spike/RBD IgG levels were measured first, third, and sixth months after the second dose. Chemiluminescent microparticle immunoassay (IgG II Quant test, Abbott, USA), which is 100% compatible with plaque reduction neutralization test, was used. Results Mean age of the participants was 38 ± 11.23 years (range between 22 and 66) of whom 119 (63.9%) were female, and 56 (32%) were male. Dramatic reductions were demonstrated in median antibody levels particularly in the infection-naïve group, comprising 138 HCWs compared to those with prior history of COVID-19 infection (n = 37) (p < 0.001). There was no difference between the two groups in terms of age, gender, blood groups, BMI, and comorbid diseases. Conclusions While antibody positivity remained above 90% in the 6th month after two doses of inactivated vaccine in HCWs, the median titers of neutralizing antibodies decreased rapidly. The decrease was more rapid and significant in those with no history of prior COVID-19 infection. In this critical phase of the pandemic, where we are facing the dominance of the Omicron variant after Delta, booster doses have become vital.

Cerrahpasa Medical Journal

Infectious Diseases and Clinical Microbiology

Vaccine, 2022

Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against... more Background Real-world studies showed varying levels of effectiveness of CoronaVac vaccine against COVID-19 disease. This study aimed to assess the association between the vaccination with CoronaVac and the COVID-19 infections among the health care workers in a university hospital and to determine the vaccine effectiveness against COVID-19 in a period when alpha variant was dominant. Methods This retrospective cohort study was conducted in a university hospital in Istanbul, Turkey employs 4067 health care workers. The follow-up period was defined as starting 14 days after receiving the second dose for fully vaccinated group. Health care workers were censored when have a positive PCR test result or at the end of the study. Unvaccinated health care workers were censored if they receive any COVID-19 vaccine doses. The incidence rate ratio and Cox regression were used to estimate the unadjusted and adjusted effectiveness of the vaccine. Findings: Seventy-one percent of the health care workers were fully vaccinated whereas 29% percent did not receive any doses. The incidence rate of SARS-CoV-2 infection was 133.7 vs 70.7 per 100.000 person-days in the unvaccinated and fully vaccinated groups, respectively. The unadjusted effectiveness against COVID-19 infection was 47% (95% CI 31–59%) whereas adjusted effectiveness was 39% (95% CI 20–64%). Interpretation: This real life study conducted in health care workers demonstrated that the effectiveness of two doses of the CoronaVac vaccine (39%) was lower than that determined in clinical trials. Due to reduce in protection over time or against variants, booster doses may be needed.

Vaccine, 2021

Healthcare workers (HCWs) were among the first groups to be vaccinated in Turkey. The data to be ... more Healthcare workers (HCWs) were among the first groups to be vaccinated in Turkey. The data to be obtained by the vaccination of HCWs would guide wide spread vaccination programs. The study included 330 HCWs working at Istanbul University-Cerrahpaşa, Cerrahpaşa Medical Faculty Hospital and vaccinated with inactive CoronaVac (Sinovac Life Sciences, China) SARS-CoV-2 vaccine in two doses (28 days apart). Anti-Spike /RBD IgG levels were measured 14 days after the first dose and 28 days after the second dose. Chemiluminescent microparticle immunoassay (CMIA) (ARCHITECT IgG II Quant test, Abbott, USA), which is 100% compatible with plaque reduction neutralization test (PRNT), was used. Of the participants, 211 (63.9%) were female, 119 (36.1%) were male, and mean age was 39.6 ± 7.7 years. In those without prior COVID-19 history; (n = 255) antibody positivity was detected as 48.2% (95% CI: 42.1-54.3) 14 days after the first dose of vaccine, and 99.2% (95% CI: 98.1-100) at day 28 after the second dose. Antibody titers were significantly lower in patients with hypertension (p = 0.011). In those with prior history of COVID-19 (n = 75); both the antibody positivity rates after the first vaccine (48.2% vs 100%, p = 0.000) and the anti-spike/RBD antibody levels after the second vaccine (with a ≥ 1050 AU/mL titer equivalent to PRNT 1/80 dilution) was significant than infection-naive group (25.9% vs. 54.7%, p = 0.000). Antibody positivity after two doses of vaccination for all study group was 99.4% (95% CI: 98.6-100). Two doses CoronaVac produce effective humoral immunity in HCWs. Antibody response is significantly higher in those with prior history of COVID-19 than infection-naive group. Given no significant benefit of the second dose, a single shot of vaccination may be sufficient for those with prior history of COVID-19. Monitoring humoral and cellular immune responses, considering new variants, is required to validate this approach.

Vaccines, 2021

Healthcare workers are among risk groups in the COVID-19. Even if they are not infected with the ... more Healthcare workers are among risk groups in the COVID-19. Even if they are not infected with the disease, they witness the effects of the pandemic. The aim of the study is to determine the factors affecting COVID-19 vaccination status and reasons for vaccine hesitancy of healthcare personnel in our hospital. Firstly, the vaccination status and demographic characteristics of all healthcare personnel was evaluated. After that, a survey was applied to 408 vaccinated and 297 nonvaccinated personnel. Within the first month after the beginning of vaccination, 66% of 3937 healthcare personnel received a COVID-19 vaccine. The number of vaccinated personnel was higher among doctors, master graduates or higher educational levels and basic science-laboratory unit workers. In the surveyed group, being under the age of 50 (OR:1.85), being nondoctor healthcare personnel (nurse/midwife OR:1.78, administrative personnel OR:3.42, patient attendant/cleaning staff OR:4.11, security guard/other OR:2.96...

Sağlık Bilimleri ve Meslekleri Dergisi, 2018

Amac: Bu calisma irritable bagirsak sendromu olan hastalarda gastrointestinal semptomlarin depres... more Amac: Bu calisma irritable bagirsak sendromu olan hastalarda gastrointestinal semptomlarin depresyon ve umutsuzluk duzeyine etkisini belirlemek amaciyla planlanmistir. Gerec ve Yontem: Calismanin yapildigi universite hastanesinin gastroenteroloji poliklinigine IBS tanisi almis gunde ortalama 3 hasta basvurmaktadir. Arastirmanin evrenini 01.03.2017-05.08.2017 tarihleri arasinda poliklinige basvuran 314 hasta, orneklemini ise 18 yas ve uzeri, IBS tanisi almis, iletisim sorunu olmayan ve arastirmaya katilmayi kabul eden 209 birey olusturmustur. Veriler hastalarin sosyodemografik ozelliklerini iceren Hasta Tanitim Formu, Gastrointestinal Semptom Degerlendirme Olcegi(GSDO, Beck Depresyon Olcegi(BDO) ve Beck Umutsuzluk Olcegi(BUO) ile toplanmistir. Verilerin degerlendirilmesinde yuzdelik, t testi, kikare testi, ANOVA ve pearson korelasyon testi kullanilmistir. Bulgular: Y as ortalamasi 40.69±14.0 olan hastalarin, ortalama hastalik suresi 6.0±1.3 yildir. Hastalarin GSDO’nden aldiklari top...

Sağlık Bilimleri ve Meslekleri Dergisi, 2018

Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve... more Bu çalışma irritable bağırsak sendromu olan hastalarda gastrointestinal semptomların depresyon ve umutsuzluk düzeyine etkisini belirlemek amacıyla planlanmıştır. Gereç ve Yöntem: Çalışmanın yapıldığı üniversite hastanesinin gastroenteroloji polikliniğine IBS tanısı almış günde ortalama 3 hasta başvurmaktadır. Araştırmanın evrenini 01.03.2017-05.08.2017 tarihleri arasında polikliniğe başvuran 314 hasta, örneklemini ise 18 yaş ve üzeri, IBS tanısı almış, iletişim sorunu olmayan ve araştırmaya katılmayı kabul eden 209 birey oluşturmuştur. Veriler hastaların sosyodemografik özelliklerini içeren Hasta Tanıtım Formu, Gastrointestinal Semptom Değerlendirme Ölçeği(GSDÖ, Beck Depresyon Ölçeği(BDÖ) ve Beck Umutsuzluk Ölçeği(BUÖ) ile toplanmıştır. Verilerin değerlendirilmesinde yüzdelik, t testi, kikare testi, ANOVA ve pearson korelasyon testi kullanılmıştır. Bulgular: Yaş ortalaması 40.69±14.0 olan hastaların, ortalama hastalık süresi 6.0±1.3 yıldır. Hastaların GSDÖ'nden aldıkları toplam puan ortalaması 52.0±1.65 olup, orta düzeyde semptom şiddetine sahip oldukları görülmüştür.. BUÖ puan ortalaması 8.0±3.1, BDS puan ortalaması 10.0±6.9 olup, hafif düzeyde umutsuzluk ve depresyon belirtisine sahip olduğu görülmüştür. GSDÖ alt boyutlarından karın ağrısı, reflü, hazımsızlık, diyare ve GSDÖ toplam puanıyla BUÖ ve BDÖ arasında anlamlı bir ilişki saptanmazken, konstipasyon alt boyut puanıyla BUÖ arasında pozitif yönde orta düzeyde bir ilişki saptanmıştır. Benzer şekilde BDÖ ile konstipasyon alt boyut puanıyla pozitif yönde güçlü düzeyde bir ilişki saptanmıştır. Sonuç: Konstipasyon ile depresyon ve umutsuzluk arasında güçlü ve orta düzeyde bir ilişki saptanmıştır. Özellikle konstipasyonu olan hastaların depresyon ve umutsuzluk düzeyi açısından değerlendirilmesi gerekmektedir.

Irish Journal of Medical Science (1971 -), 2022

Aims Despite high vaccination rates, increasing case numbers continue to be reported with the ide... more Aims Despite high vaccination rates, increasing case numbers continue to be reported with the identification of new variants of concern, and the issue of durability of the vaccine-induced immune response remains hot topic. Real-life data regarding time-dependent immunogenicity of inactivated COVID-19 vaccines are scarce. We aimed to investigate the changes in the antibody at the different times after the second dose of the CoronaVac vaccine. Methods The study included 175 HCWs vaccinated with inactive CoronaVac (Sinovac Life Sciences, China) SARS-CoV-2 vaccine in two doses. Anti-spike/RBD IgG levels were measured first, third, and sixth months after the second dose. Chemiluminescent microparticle immunoassay (IgG II Quant test, Abbott, USA), which is 100% compatible with plaque reduction neutralization test, was used. Results Mean age of the participants was 38 ± 11.23 years (range between 22 and 66) of whom 119 (63.9%) were female, and 56 (32%) were male. Dramatic reductions were demonstrated in median antibody levels particularly in the infection-naïve group, comprising 138 HCWs compared to those with prior history of COVID-19 infection (n = 37) (p < 0.001). There was no difference between the two groups in terms of age, gender, blood groups, BMI, and comorbid diseases. Conclusions While antibody positivity remained above 90% in the 6th month after two doses of inactivated vaccine in HCWs, the median titers of neutralizing antibodies decreased rapidly. The decrease was more rapid and significant in those with no history of prior COVID-19 infection. In this critical phase of the pandemic, where we are facing the dominance of the Omicron variant after Delta, booster doses have become vital.