Bita Vazir - Academia.edu (original) (raw)

Papers by Bita Vazir

Research paper thumbnail of Effects of Intracerebroventricular Injection of Spexin and Its Interaction with Nitric Oxide, Serotonin and Corticotropin Receptors on Central Food Intake Regulation in Chicken

IBRO Neuroscience Reports

Research paper thumbnail of Interactions of Cholecystokinin and Glutamatergic Systems in Feeding Behavior of Neonatal Chickens

PubMed, Apr 1, 2022

This study aimed to assess the possible feeding behavior alterations by central interactions of c... more This study aimed to assess the possible feeding behavior alterations by central interactions of cholecystokinin (CCK) and glutamatergic systems in neonatal chickens. In experiment 1, chickens received intracerebroventricular (ICV) administration of saline and CCK (CCK4; 0.25, 0.5, and 1 nmol). In experiment 2, birds were ICV injected with saline, CCK8s (0.25, 0.5, and 1 nmol). In experiment 3, chickens received the ICV injection of saline, CCK8s (1 nmol), MK-801 (15 nmol), and co-injection of the CCk8s+MK-801. Experiments 4-7 were performed similar to experiment 3, except for chickens that were injected with CNQX (390 nmol), AIDA (2 nmol), LY341495 (150 nmol), and UBP1112 (2 nmol) instead of MK-801. Subsequently, the total amount of the consumed food was determined. According to the results, the ICV administration of CCK4 (0.25, 0.5, and 1 nmol) could not affect the food intake in chickens (P>0.05). The ICV injection of the CCK8s (0.25, 0.5, and 1 nmol) led to a dose-dependent hypophagia (P<0.05). Moreover, hypophagia induced by CCK8s decreased by the co-injection of the CCK8s+MK-801 (P<0.05). These results showed that the hypophagic effects of the CCK on food intake can be mediated by NMDA glutamate receptors in layer-type chickens.

Research paper thumbnail of Evaluation of Endogenous Nitric Oxide Effect on Water Intake in Cholestatic Rats: An Experimental Study

Research paper thumbnail of GV26 Elektriksel Akupunktur Stimülasyonunun Mahmuzlu Kaplumbağanın (Testudo graeca) Anesteziden Uyanma Süresi Üzerine Değerlendirilmesi

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2023

Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing gener... more Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing general anesthesia, require prolonged monitoring period which increases the probability of postoperative complications. Therefore, prolonged recovery time following inhalant anesthesia is a common complication in chelonians. Ability to intracardiac shunting and bypassing blood from pulmonary circulation, may contribute to their unpredictable inhalant anesthetic recovery times. The acupuncture point Governing vessel (GV-26) has been demonstrated to reduce anesthetic recovery times from inhalant anesthesia in many species. In this study eight spur-thighed tortoises (Testudo gracea) were anesthetized by isoflurane administration for 60 min. The trial was performed in two parts with four weeks washout period. In the first time, once isoflurane administration was discontinued, the tortoises received no therapy in the recovery period and in the second part of the study, tortoises received GV-26 electroacupuncture stimulation. Physiologic variables, anesthetic parameters, time to first movement and time to extubation were recorded. Data were compared with the use of independent sample t tests. Tortoises receiving GV-26 electroacupuncture had a significantly reduced time to return of voluntary movement (P<0.001), and a significantly reduced time to extubation (P<0.001). Furthermore, time from first movement to extubation was significantly shorter (P<0.05). Hence, the use of GV-26 electroacupuncture results in significant reduction of anesthetic recovery time in spur-thighed tortoises which have received inhalant anesthetic.

Research paper thumbnail of Impact of the Central Histaminergic and Melanocortin Systems on Leptin-Induced Hypophagia in Neonatal Layer Chicken

PubMed, Dec 1, 2021

The present study aimed to assess the probable impact of the central histaminergic and melanocort... more The present study aimed to assess the probable impact of the central histaminergic and melanocortin systems on leptin-induced hypophagia in neonatal layer chickens. In experiment 1, the chickens received intracerebroventricular (ICV) injections of the control solution, 250 nmol of α-FMH, 10 µg of leptin, and α-FMH+leptin. Experimental groups 2-8 were injected the same as experiment 1. Nonetheless, the chickens in experiments 2-8 received ICV injections of 300 nmol of chlorpheniramine (H1 receptor antagonist), 82 nmol of famotidine (H2 receptor antagonist), 300 nmol of thioperamide (H3 receptor antagonist), 0.5 nmol of SHU9119 (M3/M4 receptors antagonist), 0.5 nmol of MCL0020 (M4 receptor antagonist), 30 µg of astressin-B (CRF1/ CRF2 receptors antagonist), and 30 µg of astressin2-B (CRF2 receptor antagonist), instead of α-FMH, respectively. Food was provided for the birds immediately following the injection, and 30, 60, and 120 min after the injection, cumulative food intake (g) was measured. The findings pointed out that the ICV injection of leptin diminished food intake in neonatal chickens (P<0.05). The co-administration of M3/M4 receptor antagonist+leptin significantly decreased the hypophagic effect of leptin (P<0.05). A significant decrease was also detected in the hypophagic effect of leptin following the co-administration of the M4 receptor antagonist and leptin (P<0.05). Moreover, the co-injection of the antagonists of CRF1/CRF2 receptors and leptin significantly mitigated the hypophagic effect of leptin (P<0.05). The co-injection of CRF2 receptor antagonist and leptin led to a decrease in the hypophagic effect of leptin. As evidenced by the results of the current study the hypophagic effect of leptin is mediated by the receptors of H1, H3, M3/M4, and CRF1/CRF2 in neonatal layer chicken.

Research paper thumbnail of Effects of berberine on pentylenetetrazole-induced seizures during estrus cycle in rats

Comparative Clinical Pathology

Research paper thumbnail of Author response for "Effects of oral propranolol on the resistive and pulsatility indices of major abdominal vasculatures in domestic short‐haired cats

Research paper thumbnail of Effects of oral propranolol on the resistive and pulsatility indices of major abdominal vasculatures in domestic short‐haired cats

Veterinary Medicine and Science

BackgroundNo study has been performed regarding the effects of oral administration of propranolol... more BackgroundNo study has been performed regarding the effects of oral administration of propranolol on pulse‐wave spectral Doppler indices of major abdominal vessels in healthy adult cats.ObjectiveThe objective of this study was to assess the pulse‐wave spectral Doppler indices of abdominal aorta, caudal vena cava, and portal vein in clinically normal adult domestic short‐haired (DSH) cats, before and after propranolol ingestion.MethodsTwenty intact adult client‐owned DSH cats were evaluated (10 males and 10 females). A duplex Doppler ultrasonography machine with a 10‐MHz frequency linear transducer was used. Peak systolic velocity, end‐diastolic velocity (EDV), resistive index (RI), pulsatility index (PI), and pressure gradient were measured. All the cats received 1 mg/kg of propranolol tablet, and after 2 h, ultrasonography measurements were repeated.ResultsThe mean RI of the aorta and caudal vena cava significantly decreased in male cats following oral administration of propranolol...

Research paper thumbnail of Role of central adiponectin and its interactions with NPY and GABAergic systems on food intake in neonatal layer chicken

Research paper thumbnail of Maternal supplementation of L-carnosine improves reflexive motor behaviors in mice offspring

Research paper thumbnail of GV26 Elektriksel Akupunktur Stimülasyonunun Mahmuzlu Kaplumbağanın (Testudo graeca) Anesteziden Uyanma Süresi Üzerine Değerlendirilmesi

Kafkas Universitesi Veteriner Fakultesi Dergisi

Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing gener... more Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing general anesthesia, require prolonged monitoring period which increases the probability of postoperative complications. Therefore, prolonged recovery time following inhalant anesthesia is a common complication in chelonians. Ability to intracardiac shunting and bypassing blood from pulmonary circulation, may contribute to their unpredictable inhalant anesthetic recovery times. The acupuncture point Governing vessel (GV-26) has been demonstrated to reduce anesthetic recovery times from inhalant anesthesia in many species. In this study eight spur-thighed tortoises (Testudo gracea) were anesthetized by isoflurane administration for 60 min. The trial was performed in two parts with four weeks washout period. In the first time, once isoflurane administration was discontinued, the tortoises received no therapy in the recovery period and in the second part of the study, tortoises received GV-26 electroacupuncture stimulation. Physiologic variables, anesthetic parameters, time to first movement and time to extubation were recorded. Data were compared with the use of independent sample t tests. Tortoises receiving GV-26 electroacupuncture had a significantly reduced time to return of voluntary movement (P<0.001), and a significantly reduced time to extubation (P<0.001). Furthermore, time from first movement to extubation was significantly shorter (P<0.05). Hence, the use of GV-26 electroacupuncture results in significant reduction of anesthetic recovery time in spur-thighed tortoises which have received inhalant anesthetic.

Research paper thumbnail of Protective effect of Crocin on Cuprizone-induced model of Multiple Sclerosis in Mice

Crocin is the main bioactive components of the saffron with neuro generative and protective activ... more Crocin is the main bioactive components of the saffron with neuro generative and protective activity, however, its activity in neurodegenerative disease in not fully elicited. So, the aim of the current study was to determine effects of the crocin on reflexive motor behavior, depressive and serum antioxidant activities on cuprizone-induced (CPZ) model of multiple sclerosis (MS) mice. 40 male C57BL/6 mice were randomly assigned into 4 groups. Mice in the control group were treated with normal diet. In group 2, CPZ-induced demyelination was done by chew palate containing 0.2% (w/w) CPZ for 5 weeks. In group 3, normal diet was provided and mice orally received crocin (100 mg/kg) 3 times per week for 5 weeks. In group 4, mice feed CPZ containing diet and orally received crocin (100 mg/kg) three times per for 5 weeks. At the end of the study, reflexive motor behavior and depressive tests were done. Also, serum and brain tissue antioxidant activity was determined. According to the data, c...

Research paper thumbnail of Mucocutaneous Bowen’s disease with a cutaneous horn overgrowth in a German shepherd dog

Comparative Clinical Pathology

Research paper thumbnail of Possible effects of the central adrenergic and dopaminergic receptors on hypophagia induced by neuromedin S in neonatal layer-type chicks

General and Comparative Endocrinology, 2022

The current study was aimed to determine the possible effects of the central adrenergic and dopam... more The current study was aimed to determine the possible effects of the central adrenergic and dopaminergic receptors in neuromedin S (NMS)-induced hypophagia in neonatal layer-type chickens. In the first experiment, control solution, and NMS (0.25, 0.5, and 1 nmol), were injected (intracerebroventricular (ICV)) in chickens. In the second experiment, birds were injected with a control solution,SCH23390 (D1receptor antagonist, 5 nmol), NMS (1 nmol), and a combination of the SCH23390 + NMS. Experiments 3-11 were similar to experiment 2, except that chickens were injected withAMI-193 (D2receptor antagonist, 5 nmol), NGB2904(D3receptor antagonist, 6.4 nmol), L-741,742(D4receptor antagonist, 6 nmol), 6-OHDA(6-hydroxydopamine, 2.5 nmol),Prazosin(α1receptor antagonist, 10 nmol),Yohimbine(α2receptor antagonist, 13 nmol),Metoprolol(β1receptor antagonist receptor, 24 nmol),ICI 118,551 (β2receptor antagonist, 5 nmol),SR 59230R (β3 receptor antagonist, 20 nmol) instead ofSCH23390. Then, cumulative food intake was recorded at 30, 60, and 120 min following the injection. According to the results, food intake was significantly decreased after ICV injection of NMS in a dose -dependent manner (P<0.05). Also, the co-injection of the SCH23390 + NMS significantly attenuated NMS-induced hypophagia (P<0.05). The co-administration of AMI-193 + NMS significantly reduced NMS- induced hypophagia (P<0.05). In addition, the co-injection of ICI 118,551 + NMS and 6-OHDA + NMS considerably decreased NMS-induced food consumption (P<0.05). However, NGB2904, L-741742, Prazosin, Yohimbine, Metoprolol and SR 59230R had no effect on hypophagia induced by NMS (P>0.05). These results demonstrated thatNMS- induced hypophagia might be mediated by D1/D2 dopaminergic andβ2adrenergic receptors in neonatal layer-type chickens.

Research paper thumbnail of Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick

The Iranian Journal of Veterinary Science and Technology, 2021

The current study aimed to see how the central dopaminergic and cannabinoidergic mechanisms affec... more The current study aimed to see how the central dopaminergic and cannabinoidergic mechanisms affect melanocortin-induced food intake in the neonatal layer chickens. In this regard, 9 experiments were designed. In experiment 1, chicks injected with control solution, MTII (2.5, 5, and 10 ng). In experiment 2, control solution, L-DOPA (125 nmol), MTII (10 ng), and L-DOPA + MTII were applied to the birds. Experiments 3-9 were similar to experiment 2, except birds injected with 6-OHDA (150 nmol), SCH23390 (5 nmol), AMI-193 (5 nmol), NGB2904 (6.4 nmol), L-741,742 (6 nmol), SR141716A (6.25 µg), and AM630 (5 µg) instead of L-DOPA. Then, cumulative food intake was recorded at 30, 60, and 120 min following injection. According to the results, in comparison with the control group, dose-dependent hypophagia was observed in 3-h food-deprived neonatal layer chickens following ICV injection of MTII (2.5, 5, and 10 ng) (P 0.05). These results demonstrated that melanocortin-induced hypophagia in the ...

Research paper thumbnail of The effect of the central administration of the Neuropeptide VF on feed intake and its possible interactions with glutamate and opioid systems in broiler chicken

Arginine-phenylalanine-amide (RFamide)-related peptides (RFRP) known as neuropeptide VF (NPVF) ha... more Arginine-phenylalanine-amide (RFamide)-related peptides (RFRP) known as neuropeptide VF (NPVF) have a crucial role in feeding regulation. The present study purposed to determine the effect of intracerebroventricular (ICV) injection of the NPVF on dietary intake and possible interactions of NPVF with glutamate and opioid systems in broiler chicken. In experiment 1, chickens received ICV administration of the control solution, NPVF (16 nmol), 15 nmol of MK-801 (NMDA glutamate receptors antagonist), and injection of the NPVF with MK-801 together. In experiments, 2-8 chicken injected intracerebroventricularly with 390 nmol of CNQX (antagonist of AMPA glutamate receptors), 2 nmol of AIDA (an antagonist of mGLUR1 glutamate receptors), 150 nmol of LY341495 (an antagonist of mGLUR2 glutamate receptors), 2 nmol of UBP1112 (an antagonist for mGLUR3 glutamate receptors), 5 µg of β-FNA (an antagonist for mu (µ) receptors), 5 µg of NTI (an antagonist for delta (δ) receptors), nor-BNI (kappa (κ) ...

Research paper thumbnail of Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens

Archives of Razi Institute, 2021

This study purposed to discover the connection between the central glutamatergic and histaminergi... more This study purposed to discover the connection between the central glutamatergic and histaminergic systems on feeding behavior in layer chickens. In the first experiment, chicks obtained intracerebroventricular (ICV) injections of saline (control solution), α-FMH (250 nmol), glutamate (300 nmol), and α-FMH + glutamate. Experiments 2-6 were comparable to the first experiment, apart from the birds being injected with chlorpheniramine (histamine H1 receptor antagonist, 300 nmol), famotidine (histamine H2 receptor antagonist, 82 nmol), and thioperamide (histamine H3 receptor antagonist, 300 nmol) instead of α-FMH. In Experiment five, experimental groups were divided into (A) control solution, (B) MK-801 (N-methyl-D-aspartate receptor antagonist, 15 nmol), (C) histamine (300 nmol) and (D) MK-801 + histamine. Experiments 6-10 and Experiment five were similar apart from the ICV injections of CNQX (AMPA receptor antagonist, 360 nm), UBP-302 (Kainate receptor antagonist, 390 nm), AIDA (mGluR...

Research paper thumbnail of Correlation of Histamine Receptors and Adrenergic Receptor in Broilers Appetite

archives of razi institute, 2021

The current study was conducted to investigate the interaction between the central adrenergic and... more The current study was conducted to investigate the interaction between the central adrenergic and histaminergic systems on the broiler chicks feed intake. In the first experiment, the control solution, 10 nmol of prazosin (an α1-receptor antagonist), 300 nmol of histamine, co-injection of prazosin and histamine, were intracerebroventricularly (ICV) injected into the chicks; experiments two to five were conducted similarly as the first experiment, in which chickens were ICV injected with 13 nmol of yohimbine (an α2-receptor antagonist), 24 nmol of metoprolol (a β1 adrenergic receptor antagonist), 5 nmol of ICI 118,551 (a β2 adrenergic receptor antagonist) and 20 nmol of SR 59230R (a β3 adrenergic receptor antagonist). In the sixth experiment, control solution, 300 nmol of noradrenaline, 250 nmol of α-FMH (an alpha fluoromethyl histidine), and noradrenaline and α-FMH were injected. Seventh to ninth experiments were similar to the sixth experiment, except the chicken ICV injected 300 n...

Research paper thumbnail of Effects of the prenatal exposure to crocin in the expression of withdrawal syndrome on reflexive motor behaviors in mice offspring’s

Neuroscience Letters, 2022

The aim of the current study was to determine effects of the prenatal exposure to crocin in the e... more The aim of the current study was to determine effects of the prenatal exposure to crocin in the expression of withdrawal syndrome on reflexive motor behaviors in mice offspring's. Fourteen male mice and 56 adult female mice were randomly divided into seven groups as: control group (morphine-abstinent male and female); group 2, drug-naïve female and morphine-abstinent male; group 3, drug-naïve male and morphine-abstinent females; group 4, drug-naïve male and female. Groups 5-7, were similar to groups 2-4, except crocin (5mg/kg) were injected to drug-naïve subjects. Following delivery, 20 pups from each litter were selected and behavior and reflexive motor behaviors were determined. Also, blood samples were taken to determine serum antioxidant activity. According to the results, immobility time significantly increased in offspring of the paternal + maternal exposed to morphine swimming test and tail suspension tests (P<0.05) and significantly decreased in offspring of paternal+maternal exposed to morphine+crocin group (P<0.05). Ambulation, surface righting, hind-limb suspension, grip strength and front limb suspension significantly decreased in offspring of the mice exposed to morphine (P<0.05) and significantly improved in offspring of paternal+maternal exposed to morphine+crocin group (P<0.05). Hind-limb foot angle and negative geotaxis significantly increased in mice with morphine-exposed offspring's (P<0.05) while improved in offspring of paternal+maternal exposed to morphine+crocin group (P<0.05). Prenatal exposure to morphine increased Malondialdehyde while decreased Superoxide dismutase, Glutathione peroxidase and total antioxidant status in mice offspring's (P<0.05) and these results reversed by prenatal exposure to crocin (P<0.05). In all studied factors, paternal+maternal exposed to morphine+crocin group had better results compared to the other crocin-received drug-naïve groups (P<0.05). These results suggested prenatal exposure to crocin decreased morphine-induced adverse effect which paternal and maternal exposed to morphine + crocin had the highest prevention against these effects in mice offspring's.

Research paper thumbnail of Interaction Between Oxytocin and Opioidergic System on Food Intake Regulation in Neonatal Layer Type Chicken

International Journal of Peptide Research and Therapeutics, 2019

The aim of the current study was to determine possible interaction of central oxytocin and opioid... more The aim of the current study was to determine possible interaction of central oxytocin and opioidergic system on food intake regulation in neonatal layer-type chicken. In experiment 1, FD 3 chicken ICV injected with control solution, oxytocin (10 µg), β-FNA (µ receptor antagonist, 5 µg) and oxytocin (10 µg) + β-FNA were injected. Experiments 2-6 were similar to experiments 1, except chicken injected with nor-BNI (κ receptor antagonist, 5 µg), NTI (δ receptor antagonist, 5 µg), DAMGO (µ receptor agonist, 62.25 pmol), U-50488H (κ receptor agonist, 10 nmol), DPDPE (δ receptor agonist, 20 pmol) instead of β-FNA. In experiment 7, control solution, DAMGO (125 pmol), d(CH2)5Tyr(Me)-[Orn8]-vasotocin (oxytocin antagonist, 5 µg) and DAMGO + d(CH2)5Tyr(Me)-[Orn8]-vasotocin were ICV injected to FD 3 chicken. Experiments 8 and 9 were similar to experiments 7, except chicken injected with U-50488H (30 nmol) and DPDPE (40 pmol) instead of DAMGO. Then, cumulative food intake was recorded at 30, 60 and 120 min after injection. According to the results, ICV injection of the oxytocin (10 µg) significantly decreased food intake compared to control group (P < 0.05). Co-injection of the oxytocin + β-FNA and oxytocin + U-50488H significantly decreased hypophagic effect of the oxytocin (P < 0.05). While, co-injection of the oxytocin + nor-BNI or oxytocin + DAMGO significantly amplified hypophagic effect of the oxytocin in chicken (P < 0.05). In addition, ICV injection of DAMGO (125 pmol) significantly decreased cumulative food intake compared to control group (P < 0.05). However, co-addministration of the DAMGO + (CH2)5Tyr(Me)-[Orn8]-vasotocin significantly decreased hypophagic effect of the DAMGO (P < 0.05) in chicken. These results suggested there are interconnection between oxytocin and opioidergic system on central food intake regulation, which mediates via µ and κ opioidergic receptors in neonatal layer-type chicken.

Research paper thumbnail of Effects of Intracerebroventricular Injection of Spexin and Its Interaction with Nitric Oxide, Serotonin and Corticotropin Receptors on Central Food Intake Regulation in Chicken

IBRO Neuroscience Reports

Research paper thumbnail of Interactions of Cholecystokinin and Glutamatergic Systems in Feeding Behavior of Neonatal Chickens

PubMed, Apr 1, 2022

This study aimed to assess the possible feeding behavior alterations by central interactions of c... more This study aimed to assess the possible feeding behavior alterations by central interactions of cholecystokinin (CCK) and glutamatergic systems in neonatal chickens. In experiment 1, chickens received intracerebroventricular (ICV) administration of saline and CCK (CCK4; 0.25, 0.5, and 1 nmol). In experiment 2, birds were ICV injected with saline, CCK8s (0.25, 0.5, and 1 nmol). In experiment 3, chickens received the ICV injection of saline, CCK8s (1 nmol), MK-801 (15 nmol), and co-injection of the CCk8s+MK-801. Experiments 4-7 were performed similar to experiment 3, except for chickens that were injected with CNQX (390 nmol), AIDA (2 nmol), LY341495 (150 nmol), and UBP1112 (2 nmol) instead of MK-801. Subsequently, the total amount of the consumed food was determined. According to the results, the ICV administration of CCK4 (0.25, 0.5, and 1 nmol) could not affect the food intake in chickens (P>0.05). The ICV injection of the CCK8s (0.25, 0.5, and 1 nmol) led to a dose-dependent hypophagia (P<0.05). Moreover, hypophagia induced by CCK8s decreased by the co-injection of the CCK8s+MK-801 (P<0.05). These results showed that the hypophagic effects of the CCK on food intake can be mediated by NMDA glutamate receptors in layer-type chickens.

Research paper thumbnail of Evaluation of Endogenous Nitric Oxide Effect on Water Intake in Cholestatic Rats: An Experimental Study

Research paper thumbnail of GV26 Elektriksel Akupunktur Stimülasyonunun Mahmuzlu Kaplumbağanın (Testudo graeca) Anesteziden Uyanma Süresi Üzerine Değerlendirilmesi

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2023

Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing gener... more Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing general anesthesia, require prolonged monitoring period which increases the probability of postoperative complications. Therefore, prolonged recovery time following inhalant anesthesia is a common complication in chelonians. Ability to intracardiac shunting and bypassing blood from pulmonary circulation, may contribute to their unpredictable inhalant anesthetic recovery times. The acupuncture point Governing vessel (GV-26) has been demonstrated to reduce anesthetic recovery times from inhalant anesthesia in many species. In this study eight spur-thighed tortoises (Testudo gracea) were anesthetized by isoflurane administration for 60 min. The trial was performed in two parts with four weeks washout period. In the first time, once isoflurane administration was discontinued, the tortoises received no therapy in the recovery period and in the second part of the study, tortoises received GV-26 electroacupuncture stimulation. Physiologic variables, anesthetic parameters, time to first movement and time to extubation were recorded. Data were compared with the use of independent sample t tests. Tortoises receiving GV-26 electroacupuncture had a significantly reduced time to return of voluntary movement (P<0.001), and a significantly reduced time to extubation (P<0.001). Furthermore, time from first movement to extubation was significantly shorter (P<0.05). Hence, the use of GV-26 electroacupuncture results in significant reduction of anesthetic recovery time in spur-thighed tortoises which have received inhalant anesthetic.

Research paper thumbnail of Impact of the Central Histaminergic and Melanocortin Systems on Leptin-Induced Hypophagia in Neonatal Layer Chicken

PubMed, Dec 1, 2021

The present study aimed to assess the probable impact of the central histaminergic and melanocort... more The present study aimed to assess the probable impact of the central histaminergic and melanocortin systems on leptin-induced hypophagia in neonatal layer chickens. In experiment 1, the chickens received intracerebroventricular (ICV) injections of the control solution, 250 nmol of α-FMH, 10 µg of leptin, and α-FMH+leptin. Experimental groups 2-8 were injected the same as experiment 1. Nonetheless, the chickens in experiments 2-8 received ICV injections of 300 nmol of chlorpheniramine (H1 receptor antagonist), 82 nmol of famotidine (H2 receptor antagonist), 300 nmol of thioperamide (H3 receptor antagonist), 0.5 nmol of SHU9119 (M3/M4 receptors antagonist), 0.5 nmol of MCL0020 (M4 receptor antagonist), 30 µg of astressin-B (CRF1/ CRF2 receptors antagonist), and 30 µg of astressin2-B (CRF2 receptor antagonist), instead of α-FMH, respectively. Food was provided for the birds immediately following the injection, and 30, 60, and 120 min after the injection, cumulative food intake (g) was measured. The findings pointed out that the ICV injection of leptin diminished food intake in neonatal chickens (P<0.05). The co-administration of M3/M4 receptor antagonist+leptin significantly decreased the hypophagic effect of leptin (P<0.05). A significant decrease was also detected in the hypophagic effect of leptin following the co-administration of the M4 receptor antagonist and leptin (P<0.05). Moreover, the co-injection of the antagonists of CRF1/CRF2 receptors and leptin significantly mitigated the hypophagic effect of leptin (P<0.05). The co-injection of CRF2 receptor antagonist and leptin led to a decrease in the hypophagic effect of leptin. As evidenced by the results of the current study the hypophagic effect of leptin is mediated by the receptors of H1, H3, M3/M4, and CRF1/CRF2 in neonatal layer chicken.

Research paper thumbnail of Effects of berberine on pentylenetetrazole-induced seizures during estrus cycle in rats

Comparative Clinical Pathology

Research paper thumbnail of Author response for "Effects of oral propranolol on the resistive and pulsatility indices of major abdominal vasculatures in domestic short‐haired cats

Research paper thumbnail of Effects of oral propranolol on the resistive and pulsatility indices of major abdominal vasculatures in domestic short‐haired cats

Veterinary Medicine and Science

BackgroundNo study has been performed regarding the effects of oral administration of propranolol... more BackgroundNo study has been performed regarding the effects of oral administration of propranolol on pulse‐wave spectral Doppler indices of major abdominal vessels in healthy adult cats.ObjectiveThe objective of this study was to assess the pulse‐wave spectral Doppler indices of abdominal aorta, caudal vena cava, and portal vein in clinically normal adult domestic short‐haired (DSH) cats, before and after propranolol ingestion.MethodsTwenty intact adult client‐owned DSH cats were evaluated (10 males and 10 females). A duplex Doppler ultrasonography machine with a 10‐MHz frequency linear transducer was used. Peak systolic velocity, end‐diastolic velocity (EDV), resistive index (RI), pulsatility index (PI), and pressure gradient were measured. All the cats received 1 mg/kg of propranolol tablet, and after 2 h, ultrasonography measurements were repeated.ResultsThe mean RI of the aorta and caudal vena cava significantly decreased in male cats following oral administration of propranolol...

Research paper thumbnail of Role of central adiponectin and its interactions with NPY and GABAergic systems on food intake in neonatal layer chicken

Research paper thumbnail of Maternal supplementation of L-carnosine improves reflexive motor behaviors in mice offspring

Research paper thumbnail of GV26 Elektriksel Akupunktur Stimülasyonunun Mahmuzlu Kaplumbağanın (Testudo graeca) Anesteziden Uyanma Süresi Üzerine Değerlendirilmesi

Kafkas Universitesi Veteriner Fakultesi Dergisi

Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing gener... more Recovery time from anesthesia can be extended in reptiles, consequently patients undergoing general anesthesia, require prolonged monitoring period which increases the probability of postoperative complications. Therefore, prolonged recovery time following inhalant anesthesia is a common complication in chelonians. Ability to intracardiac shunting and bypassing blood from pulmonary circulation, may contribute to their unpredictable inhalant anesthetic recovery times. The acupuncture point Governing vessel (GV-26) has been demonstrated to reduce anesthetic recovery times from inhalant anesthesia in many species. In this study eight spur-thighed tortoises (Testudo gracea) were anesthetized by isoflurane administration for 60 min. The trial was performed in two parts with four weeks washout period. In the first time, once isoflurane administration was discontinued, the tortoises received no therapy in the recovery period and in the second part of the study, tortoises received GV-26 electroacupuncture stimulation. Physiologic variables, anesthetic parameters, time to first movement and time to extubation were recorded. Data were compared with the use of independent sample t tests. Tortoises receiving GV-26 electroacupuncture had a significantly reduced time to return of voluntary movement (P<0.001), and a significantly reduced time to extubation (P<0.001). Furthermore, time from first movement to extubation was significantly shorter (P<0.05). Hence, the use of GV-26 electroacupuncture results in significant reduction of anesthetic recovery time in spur-thighed tortoises which have received inhalant anesthetic.

Research paper thumbnail of Protective effect of Crocin on Cuprizone-induced model of Multiple Sclerosis in Mice

Crocin is the main bioactive components of the saffron with neuro generative and protective activ... more Crocin is the main bioactive components of the saffron with neuro generative and protective activity, however, its activity in neurodegenerative disease in not fully elicited. So, the aim of the current study was to determine effects of the crocin on reflexive motor behavior, depressive and serum antioxidant activities on cuprizone-induced (CPZ) model of multiple sclerosis (MS) mice. 40 male C57BL/6 mice were randomly assigned into 4 groups. Mice in the control group were treated with normal diet. In group 2, CPZ-induced demyelination was done by chew palate containing 0.2% (w/w) CPZ for 5 weeks. In group 3, normal diet was provided and mice orally received crocin (100 mg/kg) 3 times per week for 5 weeks. In group 4, mice feed CPZ containing diet and orally received crocin (100 mg/kg) three times per for 5 weeks. At the end of the study, reflexive motor behavior and depressive tests were done. Also, serum and brain tissue antioxidant activity was determined. According to the data, c...

Research paper thumbnail of Mucocutaneous Bowen’s disease with a cutaneous horn overgrowth in a German shepherd dog

Comparative Clinical Pathology

Research paper thumbnail of Possible effects of the central adrenergic and dopaminergic receptors on hypophagia induced by neuromedin S in neonatal layer-type chicks

General and Comparative Endocrinology, 2022

The current study was aimed to determine the possible effects of the central adrenergic and dopam... more The current study was aimed to determine the possible effects of the central adrenergic and dopaminergic receptors in neuromedin S (NMS)-induced hypophagia in neonatal layer-type chickens. In the first experiment, control solution, and NMS (0.25, 0.5, and 1 nmol), were injected (intracerebroventricular (ICV)) in chickens. In the second experiment, birds were injected with a control solution,SCH23390 (D1receptor antagonist, 5 nmol), NMS (1 nmol), and a combination of the SCH23390 + NMS. Experiments 3-11 were similar to experiment 2, except that chickens were injected withAMI-193 (D2receptor antagonist, 5 nmol), NGB2904(D3receptor antagonist, 6.4 nmol), L-741,742(D4receptor antagonist, 6 nmol), 6-OHDA(6-hydroxydopamine, 2.5 nmol),Prazosin(α1receptor antagonist, 10 nmol),Yohimbine(α2receptor antagonist, 13 nmol),Metoprolol(β1receptor antagonist receptor, 24 nmol),ICI 118,551 (β2receptor antagonist, 5 nmol),SR 59230R (β3 receptor antagonist, 20 nmol) instead ofSCH23390. Then, cumulative food intake was recorded at 30, 60, and 120 min following the injection. According to the results, food intake was significantly decreased after ICV injection of NMS in a dose -dependent manner (P<0.05). Also, the co-injection of the SCH23390 + NMS significantly attenuated NMS-induced hypophagia (P<0.05). The co-administration of AMI-193 + NMS significantly reduced NMS- induced hypophagia (P<0.05). In addition, the co-injection of ICI 118,551 + NMS and 6-OHDA + NMS considerably decreased NMS-induced food consumption (P<0.05). However, NGB2904, L-741742, Prazosin, Yohimbine, Metoprolol and SR 59230R had no effect on hypophagia induced by NMS (P>0.05). These results demonstrated thatNMS- induced hypophagia might be mediated by D1/D2 dopaminergic andβ2adrenergic receptors in neonatal layer-type chickens.

Research paper thumbnail of Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick

The Iranian Journal of Veterinary Science and Technology, 2021

The current study aimed to see how the central dopaminergic and cannabinoidergic mechanisms affec... more The current study aimed to see how the central dopaminergic and cannabinoidergic mechanisms affect melanocortin-induced food intake in the neonatal layer chickens. In this regard, 9 experiments were designed. In experiment 1, chicks injected with control solution, MTII (2.5, 5, and 10 ng). In experiment 2, control solution, L-DOPA (125 nmol), MTII (10 ng), and L-DOPA + MTII were applied to the birds. Experiments 3-9 were similar to experiment 2, except birds injected with 6-OHDA (150 nmol), SCH23390 (5 nmol), AMI-193 (5 nmol), NGB2904 (6.4 nmol), L-741,742 (6 nmol), SR141716A (6.25 µg), and AM630 (5 µg) instead of L-DOPA. Then, cumulative food intake was recorded at 30, 60, and 120 min following injection. According to the results, in comparison with the control group, dose-dependent hypophagia was observed in 3-h food-deprived neonatal layer chickens following ICV injection of MTII (2.5, 5, and 10 ng) (P 0.05). These results demonstrated that melanocortin-induced hypophagia in the ...

Research paper thumbnail of The effect of the central administration of the Neuropeptide VF on feed intake and its possible interactions with glutamate and opioid systems in broiler chicken

Arginine-phenylalanine-amide (RFamide)-related peptides (RFRP) known as neuropeptide VF (NPVF) ha... more Arginine-phenylalanine-amide (RFamide)-related peptides (RFRP) known as neuropeptide VF (NPVF) have a crucial role in feeding regulation. The present study purposed to determine the effect of intracerebroventricular (ICV) injection of the NPVF on dietary intake and possible interactions of NPVF with glutamate and opioid systems in broiler chicken. In experiment 1, chickens received ICV administration of the control solution, NPVF (16 nmol), 15 nmol of MK-801 (NMDA glutamate receptors antagonist), and injection of the NPVF with MK-801 together. In experiments, 2-8 chicken injected intracerebroventricularly with 390 nmol of CNQX (antagonist of AMPA glutamate receptors), 2 nmol of AIDA (an antagonist of mGLUR1 glutamate receptors), 150 nmol of LY341495 (an antagonist of mGLUR2 glutamate receptors), 2 nmol of UBP1112 (an antagonist for mGLUR3 glutamate receptors), 5 µg of β-FNA (an antagonist for mu (µ) receptors), 5 µg of NTI (an antagonist for delta (δ) receptors), nor-BNI (kappa (κ) ...

Research paper thumbnail of Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens

Archives of Razi Institute, 2021

This study purposed to discover the connection between the central glutamatergic and histaminergi... more This study purposed to discover the connection between the central glutamatergic and histaminergic systems on feeding behavior in layer chickens. In the first experiment, chicks obtained intracerebroventricular (ICV) injections of saline (control solution), α-FMH (250 nmol), glutamate (300 nmol), and α-FMH + glutamate. Experiments 2-6 were comparable to the first experiment, apart from the birds being injected with chlorpheniramine (histamine H1 receptor antagonist, 300 nmol), famotidine (histamine H2 receptor antagonist, 82 nmol), and thioperamide (histamine H3 receptor antagonist, 300 nmol) instead of α-FMH. In Experiment five, experimental groups were divided into (A) control solution, (B) MK-801 (N-methyl-D-aspartate receptor antagonist, 15 nmol), (C) histamine (300 nmol) and (D) MK-801 + histamine. Experiments 6-10 and Experiment five were similar apart from the ICV injections of CNQX (AMPA receptor antagonist, 360 nm), UBP-302 (Kainate receptor antagonist, 390 nm), AIDA (mGluR...

Research paper thumbnail of Correlation of Histamine Receptors and Adrenergic Receptor in Broilers Appetite

archives of razi institute, 2021

The current study was conducted to investigate the interaction between the central adrenergic and... more The current study was conducted to investigate the interaction between the central adrenergic and histaminergic systems on the broiler chicks feed intake. In the first experiment, the control solution, 10 nmol of prazosin (an α1-receptor antagonist), 300 nmol of histamine, co-injection of prazosin and histamine, were intracerebroventricularly (ICV) injected into the chicks; experiments two to five were conducted similarly as the first experiment, in which chickens were ICV injected with 13 nmol of yohimbine (an α2-receptor antagonist), 24 nmol of metoprolol (a β1 adrenergic receptor antagonist), 5 nmol of ICI 118,551 (a β2 adrenergic receptor antagonist) and 20 nmol of SR 59230R (a β3 adrenergic receptor antagonist). In the sixth experiment, control solution, 300 nmol of noradrenaline, 250 nmol of α-FMH (an alpha fluoromethyl histidine), and noradrenaline and α-FMH were injected. Seventh to ninth experiments were similar to the sixth experiment, except the chicken ICV injected 300 n...

Research paper thumbnail of Effects of the prenatal exposure to crocin in the expression of withdrawal syndrome on reflexive motor behaviors in mice offspring’s

Neuroscience Letters, 2022

The aim of the current study was to determine effects of the prenatal exposure to crocin in the e... more The aim of the current study was to determine effects of the prenatal exposure to crocin in the expression of withdrawal syndrome on reflexive motor behaviors in mice offspring's. Fourteen male mice and 56 adult female mice were randomly divided into seven groups as: control group (morphine-abstinent male and female); group 2, drug-naïve female and morphine-abstinent male; group 3, drug-naïve male and morphine-abstinent females; group 4, drug-naïve male and female. Groups 5-7, were similar to groups 2-4, except crocin (5mg/kg) were injected to drug-naïve subjects. Following delivery, 20 pups from each litter were selected and behavior and reflexive motor behaviors were determined. Also, blood samples were taken to determine serum antioxidant activity. According to the results, immobility time significantly increased in offspring of the paternal + maternal exposed to morphine swimming test and tail suspension tests (P<0.05) and significantly decreased in offspring of paternal+maternal exposed to morphine+crocin group (P<0.05). Ambulation, surface righting, hind-limb suspension, grip strength and front limb suspension significantly decreased in offspring of the mice exposed to morphine (P<0.05) and significantly improved in offspring of paternal+maternal exposed to morphine+crocin group (P<0.05). Hind-limb foot angle and negative geotaxis significantly increased in mice with morphine-exposed offspring's (P<0.05) while improved in offspring of paternal+maternal exposed to morphine+crocin group (P<0.05). Prenatal exposure to morphine increased Malondialdehyde while decreased Superoxide dismutase, Glutathione peroxidase and total antioxidant status in mice offspring's (P<0.05) and these results reversed by prenatal exposure to crocin (P<0.05). In all studied factors, paternal+maternal exposed to morphine+crocin group had better results compared to the other crocin-received drug-naïve groups (P<0.05). These results suggested prenatal exposure to crocin decreased morphine-induced adverse effect which paternal and maternal exposed to morphine + crocin had the highest prevention against these effects in mice offspring's.

Research paper thumbnail of Interaction Between Oxytocin and Opioidergic System on Food Intake Regulation in Neonatal Layer Type Chicken

International Journal of Peptide Research and Therapeutics, 2019

The aim of the current study was to determine possible interaction of central oxytocin and opioid... more The aim of the current study was to determine possible interaction of central oxytocin and opioidergic system on food intake regulation in neonatal layer-type chicken. In experiment 1, FD 3 chicken ICV injected with control solution, oxytocin (10 µg), β-FNA (µ receptor antagonist, 5 µg) and oxytocin (10 µg) + β-FNA were injected. Experiments 2-6 were similar to experiments 1, except chicken injected with nor-BNI (κ receptor antagonist, 5 µg), NTI (δ receptor antagonist, 5 µg), DAMGO (µ receptor agonist, 62.25 pmol), U-50488H (κ receptor agonist, 10 nmol), DPDPE (δ receptor agonist, 20 pmol) instead of β-FNA. In experiment 7, control solution, DAMGO (125 pmol), d(CH2)5Tyr(Me)-[Orn8]-vasotocin (oxytocin antagonist, 5 µg) and DAMGO + d(CH2)5Tyr(Me)-[Orn8]-vasotocin were ICV injected to FD 3 chicken. Experiments 8 and 9 were similar to experiments 7, except chicken injected with U-50488H (30 nmol) and DPDPE (40 pmol) instead of DAMGO. Then, cumulative food intake was recorded at 30, 60 and 120 min after injection. According to the results, ICV injection of the oxytocin (10 µg) significantly decreased food intake compared to control group (P < 0.05). Co-injection of the oxytocin + β-FNA and oxytocin + U-50488H significantly decreased hypophagic effect of the oxytocin (P < 0.05). While, co-injection of the oxytocin + nor-BNI or oxytocin + DAMGO significantly amplified hypophagic effect of the oxytocin in chicken (P < 0.05). In addition, ICV injection of DAMGO (125 pmol) significantly decreased cumulative food intake compared to control group (P < 0.05). However, co-addministration of the DAMGO + (CH2)5Tyr(Me)-[Orn8]-vasotocin significantly decreased hypophagic effect of the DAMGO (P < 0.05) in chicken. These results suggested there are interconnection between oxytocin and opioidergic system on central food intake regulation, which mediates via µ and κ opioidergic receptors in neonatal layer-type chicken.