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Papers by BYEONG HEE HWANG
Journal of biotechnology, Jan 20, 2014
Hydrogenovibrio marinus, an obligate chemolithoautotroph isolated from oceanic surface water, is ... more Hydrogenovibrio marinus, an obligate chemolithoautotroph isolated from oceanic surface water, is a Knallgas bacterium that conserves energy by oxidizing H2 in the presence of O2. The strain possesses a periplasmic membrane-bound respiratory [NiFe]-hydrogenase with high O2 tolerance, hence is of great biotechnological importance in the development of H2-based technologies for a promising alternative energy. Here, we report the draft genome of H. marinus MH-110, providing genomic information on the biosynthesis of the hydrogenase, aerobic H2 metabolism, and autotrophic carbon assimilation.
ABSTRACT During implant surgeries, antibacterial agents are needed to prevent bacterial infection... more ABSTRACT During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria, and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.
Journal of Nanobiotechnology, 2021
Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseas... more Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to th...
Bulletin of the Korean Chemical Society
Journal of biotechnology, Jan 20, 2014
Hydrogenovibrio marinus, an obligate chemolithoautotroph isolated from oceanic surface water, is ... more Hydrogenovibrio marinus, an obligate chemolithoautotroph isolated from oceanic surface water, is a Knallgas bacterium that conserves energy by oxidizing H2 in the presence of O2. The strain possesses a periplasmic membrane-bound respiratory [NiFe]-hydrogenase with high O2 tolerance, hence is of great biotechnological importance in the development of H2-based technologies for a promising alternative energy. Here, we report the draft genome of H. marinus MH-110, providing genomic information on the biosynthesis of the hydrogenase, aerobic H2 metabolism, and autotrophic carbon assimilation.
ABSTRACT During implant surgeries, antibacterial agents are needed to prevent bacterial infection... more ABSTRACT During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria, and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.
Journal of Nanobiotechnology, 2021
Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseas... more Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to th...
Bulletin of the Korean Chemical Society